AT1G03980

glutathione gamma-glutamylcysteinyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006080
Physical Location & Seq
Reverse (-)
1019311 .. 1022108
2798 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G03980.2

Sequence Viewer

Length: 1104 bp
ATGCTGGAATGTTGCGAGCCGCTTGAAATAGTGAAGGATAAAGGCATTTCATTTGGAAAAGTGGTCTGTTTAGCTCATTCTTCAGGAGCAAAAGTCGAAGCTTTCCGCACAAATCAGAGCACCATTGATGATTTCCGCAAATATGTGGTCAAATGTTCAACTTCTGATAATTGTCATATGATCTCAACATATCATAGGCAAGTACTCAAGCAGACTGGAACTGGCCACTTTTCACCTATTGGTGGTTATAATGCTGAAAGAGATATGGCTTTGATTCTTGATGTCGCTCGTTTCAAGTATCCTCCTCACTGGGTTCCTCTTAAACTTCTTTGGGATGCCATGGATAGTATTGATCAGTCAACAGGGAGACGTAGAGGGTTCATGCTTATATCAAGACCCCACAGAGAACCAGGATTGCTCTATACATTAAGTTGTAAGGATGAGAGCTGGATCAGCATTGCAAAGTATTTGAAGGAAGATGTTCCTCGTCTTGTAAGCTCACAACATGTTGATACTATTGAAAGAATCTTATATGTTGTATTCAAGTCACTTCCAGCAAATTTCAACCAATTTATCAAATGGATGGCTGAGATTCGAAGAACAGAGGATGTAAATCAAAATCTTAGCTCAGAAGAGAAATCAAGGCTCAAATTAAAGCAAGAGTTACTGAAACAAGTGCAAGAAACTAAACTGTTCAAGCATGTGGATAAGTTTCTCTCCTCTGTGTACGAAGACAATCTGCCATATGTTGCTGCTAAGGTTTATTGTGACGGAGATGAAATCTTATCGGGATATGAATCAGATGAATCCTGTTGTAAGGAAACTTGTGTCAAATGTATCAAAGGTCTTGGTGAGGAGAAAGTGACAGTGGTAGCTTACCCATCCGGGAACGATGTGTTCACTGCTCTTCTGTTGGCTTTACCTCCACAGACGTGGTCAGGTATCAAAGACCAGTCACTTTTGCAAGAAATGAAACAGCTCATTTCCATGGTTAGCCACCCGACTTTGCTTCAACAAGAGGTTTTGCATCTACGACGCCAACTTGAGATGCTAAAACGATGCCAGGAGAATAAAGAAGACGAAGAACTCTCTGCTCCTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006518 GO:0006725 GO:0006790 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006820 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008324 GO:0008490 GO:0008509 GO:0009056 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010035 GO:0010038 GO:0015075 GO:0015103 GO:0015105 GO:0015291 GO:0015318 GO:0015399 GO:0015405 GO:0015446 GO:0015698 GO:0015700 GO:0016043 GO:0016143 GO:0016145 GO:0016462 GO:0016740 GO:0016746 GO:0016755 GO:0016756 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019439 GO:0019748 GO:0019757 GO:0019759 GO:0019760 GO:0019762 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0033036 GO:0033037 GO:0033554 GO:0034220 GO:0034641 GO:0042221 GO:0042343 GO:0042344 GO:0042430 GO:0042436 GO:0042545 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043043 GO:0043167 GO:0043169 GO:0043207 GO:0043225 GO:0043436 GO:0043492 GO:0043603 GO:0043604 GO:0044237 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044273 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0045229 GO:0046483 GO:0046685 GO:0046686 GO:0046700 GO:0046870 GO:0046872 GO:0046914 GO:0046937 GO:0046938 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0055085 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090662 GO:0098542 GO:0098655 GO:0098656 GO:0098660 GO:0099131 GO:0099132 GO:0099133 GO:0140096 GO:1901135 GO:1901136 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901657 GO:1901658 GO:1901683 GO:1901684
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Phytochelatin Phytochelatin_C

Protein Analysis

367

Amino Acids

41.98

Weight (kDa)

6.57

Isoelectric Point (pI)

47.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phytochelatin PF05023 7 - 79 1.2e-24 Phytochelatin synthase
Phytochelatin PF05023 99 - 234 1.5e-56 Phytochelatin synthase
Phytochelatin_C PF09328 252 - 399 8.1e-53 Domain of unknown function (DUF1984)
Phytochelatin_C PF09328 398 - 455 7.4e-21 Domain of unknown function (DUF1984)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 249
AciI CCGC 3 cut(s) 20, 106, 136
AclWI GGATC 1 cut(s) 458
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 1 cut(s) 559
AcuI CTGAAG 1 cut(s) 66
AcyI GRCGYC 1 cut(s) 1036
AfaI GTAC 2 cut(s) 204, 728
AfiI CCNNNNNNNGG 1 cut(s) 242
AflIII ACRYGT 1 cut(s) 505
AgsI TTSAA 9 cut(s) 26, 159, 295, 472, 521, 544, 565, 697, 1013
AjiI CACGTC 1 cut(s) 933
AjnI CCWGG 2 cut(s) 409, 1062
AleI CACNNNNGTG 1 cut(s) 931
AluBI AGCT 7 cut(s) 74, 101, 447, 498, 627, 875, 979
AluI AGCT 7 cut(s) 74, 101, 447, 498, 627, 875, 979
Alw21I GWGCWC 1 cut(s) 122
Alw26I GTCTC 1 cut(s) 361
AlwI GGATC 1 cut(s) 458
AoxI GGCC 1 cut(s) 223
ApeKI GCWGC 1 cut(s) 752
ApoI RAATTY 1 cut(s) 559
ArsI GACNNNNNNTTYG 2 cut(s) 132, 164
Asp700I GAANNNNTTC 1 cut(s) 480
AsuC2I CCSGG 1 cut(s) 886
AsuHPI GGTGA 2 cut(s) 225, 863
AsuII TTCGAA 1 cut(s) 595
BalI TGGCCA 1 cut(s) 225
BbsI GAAGAC 2 cut(s) 738, 1083
Bbv12I GWGCWC 1 cut(s) 122
BbvI GCAGC 1 cut(s) 739
BccI CCATC 2 cut(s) 577, 889
BciT130I CCWGG 2 cut(s) 411, 1064
BciVI GTATCC 1 cut(s) 309
BclI TGATCA 1 cut(s) 352
BcnI CCSGG 1 cut(s) 886
BcoDI GTCTC 1 cut(s) 361
BfuI GTATCC 1 cut(s) 309
BisI GCNGC 2 cut(s) 20, 753
BlsI GCNGC 2 cut(s) 21, 754
BmcAI AGTACT 1 cut(s) 204
Bme1390I CCNGG 3 cut(s) 411, 886, 1064
BmgBI CACGTC 1 cut(s) 933
BmiI GGNNCC 1 cut(s) 315
BmrFI CCNGG 3 cut(s) 411, 886, 1064
BmrI ACTGGG 1 cut(s) 319
BmsI GCATC 4 cut(s) 325, 1036, 1038, 1049
BmuI ACTGGG 1 cut(s) 319
BpiI GAAGAC 2 cut(s) 738, 1083
Bpu10I CCTNAGC 1 cut(s) 756
Bpu14I TTCGAA 1 cut(s) 595
BpuEI CTTGAG 2 cut(s) 191, 1064
BpuMI CCSGG 1 cut(s) 886
BsaBI GATNNNNATC 2 cut(s) 612, 796
BsaHI GRCGYC 1 cut(s) 1036
BsaJI CCNNGG 2 cut(s) 339, 987
BsaXI ACNNNNNCTCC 2 cut(s) 78, 108
Bsc4I CCNNNNNNNGG 1 cut(s) 242
Bse1I ACTGG 4 cut(s) 220, 226, 314, 952
Bse3DI GCAATG 1 cut(s) 456
Bse8I GATNNNNATC 2 cut(s) 612, 796
BseBI CCWGG 2 cut(s) 411, 1064
BseDI CCNNGG 2 cut(s) 339, 987
BseGI GGATG 5 cut(s) 340, 445, 588, 613, 881
BseJI GATNNNNATC 2 cut(s) 612, 796
BseLI CCNNNNNNNGG 1 cut(s) 242
BseMI GCAATG 1 cut(s) 456
BseMII CTCAG 2 cut(s) 579, 642
BseNI ACTGG 4 cut(s) 220, 226, 314, 952
BseRI GAGGAG 3 cut(s) 294, 709, 869
BseXI GCAGC 1 cut(s) 739
BshFI GGCC 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 122
BsiSI CCGG 1 cut(s) 885
BslI CCNNNNNNNGG 1 cut(s) 242
BsmAI GTCTC 1 cut(s) 361
BsmBI CGTCTC 1 cut(s) 361
BsnI GGCC 1 cut(s) 225
Bsp119I TTCGAA 1 cut(s) 595
Bsp1286I GDGCHC 1 cut(s) 122
Bsp143I GATC 3 cut(s) 180, 352, 450
Bsp19I CCATGG 2 cut(s) 339, 987
BspACI CCGC 3 cut(s) 20, 106, 136
BspANI GGCC 1 cut(s) 225
BspCNI CTCAG 2 cut(s) 580, 641
BspLI GGNNCC 1 cut(s) 315
BspPI GGATC 1 cut(s) 458
BspQI GCTCTTC 1 cut(s) 912
BspT104I TTCGAA 1 cut(s) 595
BsrDI GCAATG 1 cut(s) 456
BsrI ACTGG 4 cut(s) 220, 226, 314, 952
BssECI CCNNGG 2 cut(s) 339, 987
BssMI GATC 3 cut(s) 180, 352, 450
BssNI GRCGYC 1 cut(s) 1036
BssT1I CCWWGG 2 cut(s) 339, 987
Bst2UI CCWGG 2 cut(s) 411, 1064
Bst4CI ACNGT 2 cut(s) 693, 868
Bst6I CTCTTC 2 cut(s) 627, 912
BstACI GRCGYC 1 cut(s) 1036
BstBI TTCGAA 1 cut(s) 595
BstC8I GCNNGC 1 cut(s) 17
BstDEI CTNAG 4 cut(s) 588, 623, 628, 756
BstDSI CCRYGG 2 cut(s) 339, 987
BstF5I GGATG 5 cut(s) 340, 445, 588, 613, 881
BstKTI GATC 3 cut(s) 183, 355, 453
BstMAI GTCTC 1 cut(s) 361
BstMBI GATC 3 cut(s) 180, 352, 450
BstMWI GCNNNNNNNGC 1 cut(s) 453
BstNI CCWGG 2 cut(s) 411, 1064
BstNSI RCATGY 2 cut(s) 509, 704
BstSCI CCNGG 3 cut(s) 409, 884, 1062
BstV1I GCAGC 1 cut(s) 739
BstV2I GAAGAC 2 cut(s) 738, 1083
BstXI CCANNNNNNTGG 1 cut(s) 933
BsuI GTATCC 1 cut(s) 309
BsuRI GGCC 1 cut(s) 225
BtgI CCRYGG 2 cut(s) 339, 987
BtrI CACGTC 1 cut(s) 933
BtsCI GGATG 5 cut(s) 340, 445, 588, 613, 881
BtsI GCAGTG 1 cut(s) 900
BtsIMutI CAGTG 3 cut(s) 307, 873, 900
Cac8I GCNNGC 1 cut(s) 17
CseI GACGC 1 cut(s) 1044
Csp6I GTAC 2 cut(s) 203, 727
CviAII CATG 5 cut(s) 340, 382, 506, 701, 988
CviQI GTAC 2 cut(s) 203, 727
DdeI CTNAG 4 cut(s) 588, 623, 628, 756
DpnI GATC 3 cut(s) 182, 354, 452
DpnII GATC 3 cut(s) 180, 352, 450
EaeI YGGCCR 1 cut(s) 223
Eam1104I CTCTTC 2 cut(s) 627, 912
EarI CTCTTC 2 cut(s) 627, 912
Eco130I CCWWGG 2 cut(s) 339, 987
Eco57I CTGAAG 1 cut(s) 66
EcoRII CCWGG 2 cut(s) 409, 1062
EcoT14I CCWWGG 2 cut(s) 339, 987
ErhI CCWWGG 2 cut(s) 339, 987
Esp3I CGTCTC 1 cut(s) 361
FaeI CATG 5 cut(s) 343, 385, 509, 704, 991
FatI CATG 5 cut(s) 339, 381, 505, 700, 987
FauNDI CATATG 2 cut(s) 177, 745
FbaI TGATCA 1 cut(s) 352
Fnu4HI GCNGC 2 cut(s) 20, 753
FokI GGATG 5 cut(s) 347, 452, 595, 620, 868
Fsp4HI GCNGC 2 cut(s) 20, 753
GluI GCNGC 2 cut(s) 20, 753
HaeIII GGCC 1 cut(s) 225
HapII CCGG 1 cut(s) 885
HgaI GACGC 1 cut(s) 1044
Hin1I GRCGYC 1 cut(s) 1036
Hin1II CATG 5 cut(s) 343, 385, 509, 704, 991
HincII GTYRAC 1 cut(s) 360
HindII GTYRAC 1 cut(s) 360
HindIII AAGCTT 1 cut(s) 99
HinfI GANTC 5 cut(s) 274, 525, 592, 797, 806
HpaII CCGG 1 cut(s) 885
HphI GGTGA 2 cut(s) 225, 863
Hpy166II GTNNAC 3 cut(s) 360, 727, 900
Hpy188I TCNGA 4 cut(s) 117, 166, 631, 802
Hpy188III TCNNGA 4 cut(s) 84, 278, 393, 789
Hpy8I GTNNAC 3 cut(s) 360, 727, 900
Hpy99I CGWCG 1 cut(s) 1038
HpyAV CCTTC 2 cut(s) 28, 466
HpyCH4III ACNGT 2 cut(s) 693, 868
HpyCH4IV ACGT 2 cut(s) 370, 932
HpyCH4V TGCA 4 cut(s) 461, 679, 964, 1027
HpyF10VI GCNNNNNNNGC 1 cut(s) 453
HpyF3I CTNAG 4 cut(s) 588, 623, 628, 756
HpySE526I ACGT 2 cut(s) 370, 932
Hsp92I GRCGYC 1 cut(s) 1036
Hsp92II CATG 5 cut(s) 343, 385, 509, 704, 991
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 3 cut(s) 180, 352, 450
LguI GCTCTTC 1 cut(s) 912
LmnI GCTCC 2 cut(s) 86, 1099
Lsp1109I GCAGC 1 cut(s) 739
LweI GCATC 4 cut(s) 325, 1036, 1038, 1049
MaeII ACGT 2 cut(s) 370, 932
MaeIII GTNAC 5 cut(s) 546, 663, 767, 862, 954
MalI GATC 3 cut(s) 182, 354, 452
MboI GATC 3 cut(s) 180, 352, 450
MboII GAAGA 8 cut(s) 72, 488, 609, 644, 743, 899, 1088, 1094
MhlI GDGCHC 1 cut(s) 122
MlsI TGGCCA 1 cut(s) 225
MluCI AATT 4 cut(s) 169, 559, 569, 650
MluNI TGGCCA 1 cut(s) 225
Mox20I TGGCCA 1 cut(s) 225
MroXI GAANNNNTTC 1 cut(s) 480
MscI TGGCCA 1 cut(s) 225
MseI TTAA 3 cut(s) 321, 428, 653
MslI CAYNNNNRTG 2 cut(s) 931, 986
Msp20I TGGCCA 1 cut(s) 225
MspI CCGG 1 cut(s) 885
MspR9I CCNGG 3 cut(s) 411, 886, 1064
MvaI CCWGG 2 cut(s) 411, 1064
MwoI GCNNNNNNNGC 1 cut(s) 453
NciI CCSGG 1 cut(s) 886
NcoI CCATGG 2 cut(s) 339, 987
NdeI CATATG 2 cut(s) 177, 745
NdeII GATC 3 cut(s) 180, 352, 450
NlaIII CATG 5 cut(s) 343, 385, 509, 704, 991
NlaIV GGNNCC 1 cut(s) 315
NmuCI GTSAC 4 cut(s) 546, 767, 862, 954
NspI RCATGY 2 cut(s) 509, 704
NspV TTCGAA 1 cut(s) 595
OliI CACNNNNGTG 1 cut(s) 931
PciI ACATGT 1 cut(s) 505
PciSI GCTCTTC 1 cut(s) 912
PdmI GAANNNNTTC 1 cut(s) 480
PfeI GAWTC 5 cut(s) 274, 525, 592, 797, 806
PflFI GACNNNGTC 1 cut(s) 934
PfoI TCCNGGA 1 cut(s) 884
PkrI GCNGC 2 cut(s) 21, 754
PscI ACATGT 1 cut(s) 505
PsiI TTATAA 1 cut(s) 249
Psp6I CCWGG 2 cut(s) 409, 1062
PspGI CCWGG 2 cut(s) 409, 1062
PspN4I GGNNCC 1 cut(s) 315
PsyI GACNNNGTC 1 cut(s) 934
RsaI GTAC 2 cut(s) 204, 728
RsaNI GTAC 2 cut(s) 203, 727
RseI CAYNNNNRTG 2 cut(s) 931, 986
SapI GCTCTTC 1 cut(s) 912
SaqAI TTAA 3 cut(s) 321, 428, 653
SatI GCNGC 2 cut(s) 20, 753
Sau3AI GATC 3 cut(s) 180, 352, 450
ScaI AGTACT 1 cut(s) 204
ScrFI CCNGG 3 cut(s) 411, 886, 1064
SduI GDGCHC 1 cut(s) 122
SfaNI GCATC 4 cut(s) 325, 1036, 1038, 1049
SfuI TTCGAA 1 cut(s) 595
SmiMI CAYNNNNRTG 2 cut(s) 931, 986
SmlI CTYRAG 2 cut(s) 206, 1043
SmoI CTYRAG 2 cut(s) 206, 1043
Sse9I AATT 4 cut(s) 169, 559, 569, 650
SsiI CCGC 3 cut(s) 20, 106, 136
StyD4I CCNGG 3 cut(s) 409, 884, 1062
StyI CCWWGG 2 cut(s) 339, 987
TaaI ACNGT 2 cut(s) 693, 868
TaiI ACGT 2 cut(s) 373, 935
TaqI TCGA 2 cut(s) 96, 595
TasI AATT 4 cut(s) 169, 559, 569, 650
TatI WGTACW 1 cut(s) 202
TauI GCSGC 1 cut(s) 22
TfiI GAWTC 5 cut(s) 274, 525, 592, 797, 806
Tru1I TTAA 3 cut(s) 321, 428, 653
Tru9I TTAA 3 cut(s) 321, 428, 653
TscAI CASTG 3 cut(s) 314, 873, 907
TseFI GTSAC 4 cut(s) 546, 767, 862, 954
TseI GCWGC 1 cut(s) 752
Tsp45I GTSAC 4 cut(s) 546, 767, 862, 954
TspDTI ATGAA 6 cut(s) 39, 370, 792, 810, 819, 986
TspGWI ACGGA 1 cut(s) 786
TspRI CASTG 3 cut(s) 314, 873, 907
Tth111I GACNNNGTC 1 cut(s) 934
XapI RAATTY 1 cut(s) 559
XceI RCATGY 2 cut(s) 509, 704
XmnI GAANNNNTTC 1 cut(s) 480
ZrmI AGTACT 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.