MD05G1193400.v1.1

Glutathione gamma-glutamylcysteinyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006080
Physical Location & Seq
Reverse (-)
32114367 .. 32124009
9643 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1193400.v1.1.491

Sequence Viewer

Length: 1494 bp
ATGGCTATGGCGGGGCTGTACAGACGAACCCTTCCTTCTCCTCCGGCAGTCGAATTTGCTTCCTTACAGGGAAAGGAGCTTTTTCTTGAAGCCATTCAGAGGGGAACTATGGAGAACTTCTATAGGTTGATTTCTTATTTCCAGACACAATCCGAGCCCGCTTATTGCGGATTGGCGAGCCTGTCCATGGTTTTGAATGCCCTTGCCATTGATCCCGGAAGGAAATGGAAAGGACCCTGGAGATGGTTTGATGAATCTATGTTAGACTGCTGCGAGCCTTTGGAGACTGTCAAGGTAAAAGGCATCTCATTTGGGAAGCTAGTCTGCTTGGCTCACTGTGCTGGAGCAAAAGTTGAAGCCTTTCGCACAAATCATAGCACAATTGATGAGTTTCGTAAATACGTATTGAGATGTTCTACTTCTGATGATTGTCATGTGATCTCCTCATACGATAGATCAGTTCTCAAACAGACGGGTACTGGTCATTTTTCACCAATTGGTGGCTATCATGCTGAAAGAGACATGGCGCTTATTTTGGATGTTGCACGTTTTAAGTATCCTCCCCATTGGGTACCCCTCAAAATTCTTTGGGACGCCATGAATAATGTTGATTCATCTACTGGACAACGCAGAGGGTTCATGCTTATATCCAGGCCTCACAGCGAGCCAGGACAACTTTATACCCTGAGCTGCAAGCATGAGAGTTGGGTTGGTGTTGCGAAATACTTAATGGATGATGTTCCTCTTCTTCTAAAATCGGAGGAGGTGAAAGACATTCAGGAAGTTTTCTCTGTTGTTTTTATGTCGCTCCCATCCTATTTTGGGCAGTTCATCAAGTGGGTTGCAGAAGTTCGGAGACGAGAGGATGGTGGCCAAAGCTTAAGCCTAGAGGAAAAAGCTAGGCTTGCTGTCAAGGAGGAGGTACTGAGACAAGTCCATGACACTGGCCTTTTTAAACACGTGGCAGAATTATTATCTTCAGCACATTCATGCTGCGGAAACTTATATTCCGGTCATGAAGAAAACTTGCCCAACATTGCTGCGAGCGTTTGCTGCCAAGGCGCACAAATTTTGTCGGGGAACTCTGCCTTCTCGGGGGTGTATTGCTGTCAGGAAACATGTGTGAAATGCTTCAAGGCAAATGGTGACAAGCCAGTCACGGTTGTGAGTGGGACAGTGGTAAATGGTGGAACCGAGGAGAGGATGGACGTGCTTGTTCCTTCATCAAAAACAAATTCAGGATGTTCTTGTGCAATTGGGATGCATCCAGCTAGCAATGACGTTCTAACAGCACTTCTACTGGCCTTGCCACCAGGGACATGGTCTGGGATCAAAGACGAGAAGCTTTCAAAAGAAATATCTAACCTTGTTTCCACTGTAAATCTTCCTACCTTGCTTCAAGAAGAGGTTTTGCACTTGCGACGCCAGCTCCACCTCCTCAAGAAATGCCAAGAGGATAGGGTAGACGAAGATCTTGGCTCTCCTCTCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006518 GO:0006725 GO:0006790 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006820 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008324 GO:0008490 GO:0008509 GO:0009056 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010035 GO:0010038 GO:0015075 GO:0015103 GO:0015105 GO:0015291 GO:0015318 GO:0015399 GO:0015405 GO:0015446 GO:0015698 GO:0015700 GO:0016043 GO:0016143 GO:0016145 GO:0016462 GO:0016740 GO:0016746 GO:0016755 GO:0016756 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019439 GO:0019748 GO:0019757 GO:0019759 GO:0019760 GO:0019762 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0033036 GO:0033037 GO:0033554 GO:0034220 GO:0034641 GO:0042221 GO:0042343 GO:0042344 GO:0042430 GO:0042436 GO:0042545 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043043 GO:0043167 GO:0043169 GO:0043207 GO:0043225 GO:0043436 GO:0043492 GO:0043603 GO:0043604 GO:0044237 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044273 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0045229 GO:0046483 GO:0046685 GO:0046686 GO:0046700 GO:0046870 GO:0046872 GO:0046914 GO:0046937 GO:0046938 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0055085 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090662 GO:0098542 GO:0098655 GO:0098656 GO:0098660 GO:0099131 GO:0099132 GO:0099133 GO:0140096 GO:1901135 GO:1901136 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901657 GO:1901658 GO:1901683 GO:1901684
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Phytochelatin Phytochelatin_C

Protein Analysis

498

Amino Acids

55.06

Weight (kDa)

6.33

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phytochelatin PF05023 7 - 213 1.3e-89 Phytochelatin synthase
Phytochelatin_C PF09328 231 - 477 5e-121 Domain of unknown function (DUF1984)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1154
Acc65I GGTACC 1 cut(s) 571
AccB1I GGYRCC 1 cut(s) 571
AccB7I CCANNNNNTGG 1 cut(s) 500
AccI GTMKAC 1 cut(s) 1464
AciI CCGC 4 cut(s) 11, 159, 168, 996
AclWI GGATC 2 cut(s) 206, 1337
AcoI YGGCCR 1 cut(s) 871
AcsI RAATTY 4 cut(s) 53, 582, 1068, 1234
AcuI CTGAAG 1 cut(s) 963
AcvI CACGTG 1 cut(s) 961
AcyI GRCGYC 2 cut(s) 594, 1423
AfaI GTAC 4 cut(s) 20, 478, 573, 924
AfiI CCNNNNNNNGG 7 cut(s) 99, 187, 243, 500, 822, 1095, 1200
AflII CTTAAG 1 cut(s) 880
AflIII ACRYGT 2 cut(s) 958, 1118
AgsI TTSAA 6 cut(s) 89, 196, 356, 1135, 1350, 1400
AjiI CACGTC 1 cut(s) 1210
AjnI CCWGG 4 cut(s) 236, 650, 667, 1312
AleI CACNNNNGTG 1 cut(s) 1163
AluBI AGCT 8 cut(s) 79, 319, 690, 879, 899, 1271, 1345, 1429
AluI AGCT 8 cut(s) 79, 319, 690, 879, 899, 1271, 1345, 1429
Alw26I GTCTC 4 cut(s) 278, 513, 850, 922
AlwI GGATC 2 cut(s) 206, 1337
Ama87I CYCGRG 1 cut(s) 1093
AoxI GGCC 4 cut(s) 653, 871, 946, 1302
ApeKI GCWGC 5 cut(s) 270, 690, 993, 1040, 1053
ApoI RAATTY 4 cut(s) 53, 582, 1068, 1234
Asp700I GAANNNNTTC 3 cut(s) 93, 360, 1130
Asp718I GGTACC 1 cut(s) 571
AspLEI GCGC 2 cut(s) 529, 1064
AspS9I GGNCC 1 cut(s) 233
AsuC2I CCSGG 1 cut(s) 216
AsuHPI GGTGA 3 cut(s) 483, 778, 1157
AsuNHI GCTAGC 1 cut(s) 1271
AvaI CYCGRG 1 cut(s) 1093
AvaII GGWCC 1 cut(s) 233
BalI TGGCCA 1 cut(s) 873
BanI GGYRCC 1 cut(s) 571
BanII GRGCYC 1 cut(s) 159
BbrPI CACGTG 1 cut(s) 961
BbvI GCAGC 5 cut(s) 257, 677, 980, 1027, 1040
BccI CCATC 4 cut(s) 237, 820, 860, 1198
BciT130I CCWGG 4 cut(s) 238, 652, 669, 1314
BciVI GTATCC 1 cut(s) 567
BcnI CCSGG 1 cut(s) 216
BcoDI GTCTC 4 cut(s) 278, 513, 850, 922
BfaI CTAG 4 cut(s) 320, 887, 900, 1272
BfmI CTRYAG 1 cut(s) 121
BfoI RGCGCY 1 cut(s) 530
BfrI CTTAAG 1 cut(s) 880
BfuI GTATCC 1 cut(s) 567
BglII AGATCT 1 cut(s) 1471
BisI GCNGC 5 cut(s) 271, 691, 994, 1041, 1054
BlsI GCNGC 5 cut(s) 272, 692, 995, 1042, 1055
Bme1390I CCNGG 5 cut(s) 216, 238, 652, 669, 1314
Bme18I GGWCC 1 cut(s) 233
BmeT110I CYCGRG 1 cut(s) 1093
BmgBI CACGTC 1 cut(s) 1210
BmgT120I GGNCC 1 cut(s) 233
BmiI GGNNCC 3 cut(s) 235, 573, 1192
BmrFI CCNGG 5 cut(s) 216, 238, 652, 669, 1314
BmsI GCATC 3 cut(s) 312, 1251, 1273
BmtI GCTAGC 1 cut(s) 1275
BpmI CTGGAG 2 cut(s) 259, 363
Bpu10I CCTNAGC 1 cut(s) 686
BpuEI CTTGAG 1 cut(s) 1424
BpuMI CCSGG 1 cut(s) 216
BsaAI YACGTR 2 cut(s) 403, 961
BsaHI GRCGYC 2 cut(s) 594, 1423
BsaJI CCNNGG 5 cut(s) 186, 236, 1057, 1194, 1313
BsaWI WCCGGW 1 cut(s) 1010
BsaXI ACNNNNNCTCC 4 cut(s) 336, 366, 1413, 1443
Bsc4I CCNNNNNNNGG 7 cut(s) 99, 187, 243, 500, 822, 1095, 1200
Bse1I ACTGG 5 cut(s) 484, 625, 949, 1154, 1305
Bse3DI GCAATG 2 cut(s) 1035, 1282
BseBI CCWGG 4 cut(s) 238, 652, 669, 1314
BseDI CCNNGG 5 cut(s) 186, 236, 1057, 1194, 1313
BseGI GGATG 8 cut(s) 544, 739, 812, 871, 1209, 1247, 1264, 1266
BseLI CCNNNNNNNGG 7 cut(s) 99, 187, 243, 500, 822, 1095, 1200
BseMI GCAATG 2 cut(s) 1035, 1282
BseMII CTCAG 2 cut(s) 677, 917
BseNI ACTGG 5 cut(s) 484, 625, 949, 1154, 1305
BseRI GAGGAG 7 cut(s) 30, 433, 776, 932, 1211, 1427, 1473
BseXI GCAGC 5 cut(s) 257, 677, 980, 1027, 1040
BshFI GGCC 4 cut(s) 655, 873, 948, 1304
BshNI GGYRCC 1 cut(s) 571
BsiHKCI CYCGRG 1 cut(s) 1093
BsiSI CCGG 3 cut(s) 44, 216, 1011
BslFI GGGAC 3 cut(s) 605, 1186, 1330
BslI CCNNNNNNNGG 7 cut(s) 99, 187, 243, 500, 822, 1095, 1200
BsmAI GTCTC 4 cut(s) 278, 513, 850, 922
BsmBI CGTCTC 1 cut(s) 850
BsmFI GGGAC 3 cut(s) 605, 1186, 1330
BsmI GAATGC 1 cut(s) 202
BsnI GGCC 4 cut(s) 655, 873, 948, 1304
BsoBI CYCGRG 1 cut(s) 1093
Bsp1286I GDGCHC 1 cut(s) 159
Bsp1407I TGTACA 1 cut(s) 18
Bsp143I GATC 5 cut(s) 211, 438, 455, 1329, 1471
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 4 cut(s) 11, 159, 168, 996
BspANI GGCC 4 cut(s) 655, 873, 948, 1304
BspCNI CTCAG 2 cut(s) 678, 918
BspHI TCATGA 1 cut(s) 1015
BspLI GGNNCC 3 cut(s) 235, 573, 1192
BspOI GCTAGC 1 cut(s) 1275
BspPI GGATC 2 cut(s) 206, 1337
BspT107I GGYRCC 1 cut(s) 571
BspTI CTTAAG 1 cut(s) 880
BsrDI GCAATG 2 cut(s) 1035, 1282
BsrGI TGTACA 1 cut(s) 18
BsrI ACTGG 5 cut(s) 484, 625, 949, 1154, 1305
BssECI CCNNGG 5 cut(s) 186, 236, 1057, 1194, 1313
BssMI GATC 5 cut(s) 211, 438, 455, 1329, 1471
BssNI GRCGYC 2 cut(s) 594, 1423
BssT1I CCWWGG 2 cut(s) 186, 1057
Bst2UI CCWGG 4 cut(s) 238, 652, 669, 1314
Bst4CI ACNGT 5 cut(s) 289, 338, 1162, 1177, 1378
Bst6I CTCTTC 2 cut(s) 750, 1398
BstACI GRCGYC 2 cut(s) 594, 1423
BstAFI CTTAAG 1 cut(s) 880
BstAUI TGTACA 1 cut(s) 18
BstBAI YACGTR 2 cut(s) 403, 961
BstC8I GCNNGC 9 cut(s) 159, 178, 275, 665, 695, 906, 1045, 1273, 1427
BstDEI CTNAG 3 cut(s) 686, 926, 1491
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 8 cut(s) 544, 739, 812, 871, 1209, 1247, 1264, 1266
BstH2I RGCGCY 1 cut(s) 530
BstHHI GCGC 2 cut(s) 529, 1064
BstKTI GATC 5 cut(s) 214, 441, 458, 1332, 1474
BstMAI GTCTC 4 cut(s) 278, 513, 850, 922
BstMBI GATC 5 cut(s) 211, 438, 455, 1329, 1471
BstMWI GCNNNNNNNGC 5 cut(s) 338, 905, 1053, 1059, 1426
BstNI CCWGG 4 cut(s) 238, 652, 669, 1314
BstNSI RCATGY 1 cut(s) 1122
BstSCI CCNGG 5 cut(s) 214, 236, 650, 667, 1312
BstSFI CTRYAG 1 cut(s) 121
BstSNI TACGTA 1 cut(s) 403
BstV1I GCAGC 5 cut(s) 257, 677, 980, 1027, 1040
BstX2I RGATCY 1 cut(s) 1471
BstXI CCANNNNNNTGG 2 cut(s) 944, 1320
BstYI RGATCY 1 cut(s) 1471
BsuI GTATCC 1 cut(s) 567
BsuRI GGCC 4 cut(s) 655, 873, 948, 1304
BtgI CCRYGG 1 cut(s) 186
BtrI CACGTC 1 cut(s) 1210
BtsCI GGATG 8 cut(s) 544, 739, 812, 871, 1209, 1247, 1264, 1266
BtsIMutI CAGTG 4 cut(s) 334, 942, 1182, 1374
Cac8I GCNNGC 9 cut(s) 159, 178, 275, 665, 695, 906, 1045, 1273, 1427
CciI TCATGA 1 cut(s) 1015
CfoI GCGC 2 cut(s) 529, 1064
Cfr13I GGNCC 1 cut(s) 233
CseI GACGC 2 cut(s) 602, 1431
Csp6I GTAC 4 cut(s) 19, 477, 572, 923
CviQI GTAC 4 cut(s) 19, 477, 572, 923
DdeI CTNAG 3 cut(s) 686, 926, 1491
DpnI GATC 5 cut(s) 213, 440, 457, 1331, 1473
DpnII GATC 5 cut(s) 211, 438, 455, 1329, 1471
DraI TTTAAA 1 cut(s) 955
DrdI GACNNNNNNGTC 1 cut(s) 1154
DseDI GACNNNNNNGTC 1 cut(s) 1154
EaeI YGGCCR 1 cut(s) 871
Eam1104I CTCTTC 2 cut(s) 750, 1398
EarI CTCTTC 2 cut(s) 750, 1398
Eco105I TACGTA 1 cut(s) 403
Eco130I CCWWGG 2 cut(s) 186, 1057
Eco147I AGGCCT 1 cut(s) 655
Eco24I GRGCYC 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 233
Eco57I CTGAAG 1 cut(s) 963
Eco72I CACGTG 1 cut(s) 961
Eco88I CYCGRG 1 cut(s) 1093
EcoO109I RGGNCCY 1 cut(s) 233
EcoRII CCWGG 4 cut(s) 236, 650, 667, 1312
EcoT14I CCWWGG 2 cut(s) 186, 1057
EcoT22I ATGCAT 1 cut(s) 1266
EcoT38I GRGCYC 1 cut(s) 159
ErhI CCWWGG 2 cut(s) 186, 1057
Esp3I CGTCTC 1 cut(s) 850
FalI AAGNNNNNCTT 2 cut(s) 888, 920
FaqI GGGAC 3 cut(s) 605, 1186, 1330
FauI CCCGC 2 cut(s) 4, 166
FblI GTMKAC 1 cut(s) 1464
Fnu4HI GCNGC 5 cut(s) 271, 691, 994, 1041, 1054
FokI GGATG 8 cut(s) 551, 746, 799, 878, 1216, 1251, 1254, 1273
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 5 cut(s) 271, 691, 994, 1041, 1054
FspBI CTAG 4 cut(s) 320, 887, 900, 1272
GlaI GCGC 2 cut(s) 528, 1063
GluI GCNGC 5 cut(s) 271, 691, 994, 1041, 1054
GsuI CTGGAG 2 cut(s) 259, 363
HaeII RGCGCY 1 cut(s) 530
HaeIII GGCC 4 cut(s) 655, 873, 948, 1304
HapII CCGG 3 cut(s) 44, 216, 1011
HgaI GACGC 2 cut(s) 602, 1431
HhaI GCGC 2 cut(s) 529, 1064
Hin1I GRCGYC 2 cut(s) 594, 1423
Hin6I GCGC 2 cut(s) 527, 1062
HinP1I GCGC 2 cut(s) 527, 1062
HindIII AAGCTT 2 cut(s) 877, 1343
HinfI GANTC 2 cut(s) 254, 611
HpaII CCGG 3 cut(s) 44, 216, 1011
HphI GGTGA 3 cut(s) 483, 778, 1157
Hpy166II GTNNAC 1 cut(s) 1465
Hpy188I TCNGA 5 cut(s) 99, 154, 424, 760, 855
Hpy188III TCNNGA 8 cut(s) 86, 142, 779, 1016, 1112, 1239, 1400, 1441
Hpy8I GTNNAC 1 cut(s) 1465
Hpy99I CGWCG 1 cut(s) 1425
HpyAV CCTTC 5 cut(s) 41, 45, 213, 1099, 1230
HpyCH4III ACNGT 5 cut(s) 289, 338, 1162, 1177, 1378
HpyCH4IV ACGT 5 cut(s) 402, 547, 960, 1209, 1281
HpyCH4V TGCA 6 cut(s) 545, 693, 845, 1253, 1264, 1414
HpyF10VI GCNNNNNNNGC 5 cut(s) 338, 905, 1053, 1059, 1426
HpyF3I CTNAG 3 cut(s) 686, 926, 1491
HpySE526I ACGT 5 cut(s) 402, 547, 960, 1209, 1281
Hsp92I GRCGYC 2 cut(s) 594, 1423
HspAI GCGC 2 cut(s) 527, 1062
KpnI GGTACC 1 cut(s) 575
Kzo9I GATC 5 cut(s) 211, 438, 455, 1329, 1471
LmnI GCTCC 4 cut(s) 76, 344, 813, 1434
Lsp1109I GCAGC 5 cut(s) 257, 677, 980, 1027, 1040
LweI GCATC 3 cut(s) 312, 1251, 1273
MaeI CTAG 4 cut(s) 320, 887, 900, 1272
MaeII ACGT 5 cut(s) 402, 547, 960, 1209, 1281
MaeIII GTNAC 2 cut(s) 1145, 1156
MalI GATC 5 cut(s) 213, 440, 457, 1331, 1473
MboI GATC 5 cut(s) 211, 438, 455, 1329, 1471
MboII GAAGA 7 cut(s) 737, 740, 969, 1031, 1376, 1415, 1481
MfeI CAATTG 3 cut(s) 381, 495, 1254
MflI RGATCY 1 cut(s) 1471
MhlI GDGCHC 1 cut(s) 159
MlsI TGGCCA 1 cut(s) 873
MluCI AATT 8 cut(s) 53, 381, 495, 582, 968, 1068, 1234, 1254
MluNI TGGCCA 1 cut(s) 873
Mox20I TGGCCA 1 cut(s) 873
Mph1103I ATGCAT 1 cut(s) 1266
MroXI GAANNNNTTC 3 cut(s) 93, 360, 1130
MscI TGGCCA 1 cut(s) 873
MseI TTAA 4 cut(s) 552, 728, 881, 954
MslI CAYNNNNRTG 2 cut(s) 988, 1163
Msp20I TGGCCA 1 cut(s) 873
MspCI CTTAAG 1 cut(s) 880
MspI CCGG 3 cut(s) 44, 216, 1011
MspR9I CCNGG 5 cut(s) 216, 238, 652, 669, 1314
MunI CAATTG 3 cut(s) 381, 495, 1254
Mva1269I GAATGC 1 cut(s) 202
MvaI CCWGG 4 cut(s) 238, 652, 669, 1314
MwoI GCNNNNNNNGC 5 cut(s) 338, 905, 1053, 1059, 1426
NciI CCSGG 1 cut(s) 216
NcoI CCATGG 1 cut(s) 186
NdeII GATC 5 cut(s) 211, 438, 455, 1329, 1471
NheI GCTAGC 1 cut(s) 1271
NlaIV GGNNCC 3 cut(s) 235, 573, 1192
NmuCI GTSAC 2 cut(s) 1145, 1156
NsiI ATGCAT 1 cut(s) 1266
NspI RCATGY 1 cut(s) 1122
OliI CACNNNNGTG 1 cut(s) 1163
PagI TCATGA 1 cut(s) 1015
PceI AGGCCT 1 cut(s) 655
PciI ACATGT 1 cut(s) 1118
PctI GAATGC 1 cut(s) 202
PdmI GAANNNNTTC 3 cut(s) 93, 360, 1130
PfeI GAWTC 2 cut(s) 254, 611
PflFI GACNNNGTC 1 cut(s) 1321
PflMI CCANNNNNTGG 1 cut(s) 500
PfoI TCCNGGA 1 cut(s) 214
PkrI GCNGC 5 cut(s) 272, 692, 995, 1042, 1055
PmaCI CACGTG 1 cut(s) 961
PmlI CACGTG 1 cut(s) 961
Ppu21I YACGTR 2 cut(s) 403, 961
PpuMI RGGWCCY 1 cut(s) 233
PscI ACATGT 1 cut(s) 1118
Psp5II RGGWCCY 1 cut(s) 233
Psp6I CCWGG 4 cut(s) 236, 650, 667, 1312
PspCI CACGTG 1 cut(s) 961
PspGI CCWGG 4 cut(s) 236, 650, 667, 1312
PspN4I GGNNCC 3 cut(s) 235, 573, 1192
PspPI GGNCC 1 cut(s) 233
PspPPI RGGWCCY 1 cut(s) 233
PsuI RGATCY 1 cut(s) 1471
PsyI GACNNNGTC 1 cut(s) 1321
RsaI GTAC 4 cut(s) 20, 478, 573, 924
RsaNI GTAC 4 cut(s) 19, 477, 572, 923
RseI CAYNNNNRTG 2 cut(s) 988, 1163
SaqAI TTAA 4 cut(s) 552, 728, 881, 954
SatI GCNGC 5 cut(s) 271, 691, 994, 1041, 1054
Sau3AI GATC 5 cut(s) 211, 438, 455, 1329, 1471
Sau96I GGNCC 1 cut(s) 233
ScrFI CCNGG 5 cut(s) 216, 238, 652, 669, 1314
SduI GDGCHC 1 cut(s) 159
SfaNI GCATC 3 cut(s) 312, 1251, 1273
SfcI CTRYAG 1 cut(s) 121
SinI GGWCC 1 cut(s) 233
SmiMI CAYNNNNRTG 2 cut(s) 988, 1163
SmlI CTYRAG 2 cut(s) 880, 1439
SmoI CTYRAG 2 cut(s) 880, 1439
SnaBI TACGTA 1 cut(s) 403
Sse9I AATT 8 cut(s) 53, 381, 495, 582, 968, 1068, 1234, 1254
SseBI AGGCCT 1 cut(s) 655
SsiI CCGC 4 cut(s) 11, 159, 168, 996
SspMI CTAG 4 cut(s) 320, 887, 900, 1272
StuI AGGCCT 1 cut(s) 655
StyD4I CCNGG 5 cut(s) 214, 236, 650, 667, 1312
StyI CCWWGG 2 cut(s) 186, 1057
TaaI ACNGT 5 cut(s) 289, 338, 1162, 1177, 1378
TaiI ACGT 5 cut(s) 405, 550, 963, 1212, 1284
TaqI TCGA 1 cut(s) 51
TasI AATT 8 cut(s) 53, 381, 495, 582, 968, 1068, 1234, 1254
TatI WGTACW 1 cut(s) 18
TfiI GAWTC 2 cut(s) 254, 611
Tru1I TTAA 4 cut(s) 552, 728, 881, 954
Tru9I TTAA 4 cut(s) 552, 728, 881, 954
TscAI CASTG 4 cut(s) 341, 949, 1182, 1381
TseFI GTSAC 2 cut(s) 1145, 1156
TseI GCWGC 5 cut(s) 270, 690, 993, 1040, 1053
Tsp45I GTSAC 2 cut(s) 1145, 1156
TspDTI ATGAA 8 cut(s) 267, 603, 614, 628, 820, 978, 1032, 1212
TspRI CASTG 4 cut(s) 341, 949, 1182, 1381
Tth111I GACNNNGTC 1 cut(s) 1321
Van91I CCANNNNNTGG 1 cut(s) 500
Vha464I CTTAAG 1 cut(s) 880
VpaK11BI GGWCC 1 cut(s) 233
XapI RAATTY 4 cut(s) 53, 582, 1068, 1234
XceI RCATGY 1 cut(s) 1122
XcmI CCANNNNNNNNNTGG 1 cut(s) 1317
XmiI GTMKAC 1 cut(s) 1464
XmnI GAANNNNTTC 3 cut(s) 93, 360, 1130
XspI CTAG 4 cut(s) 320, 887, 900, 1272
Zsp2I ATGCAT 1 cut(s) 1266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.