Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0006080

Physical Location & Seq

Genomic Coordinates Reverse (-)

27347376 .. 27350973

Gene Structure 3598 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1180000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1488 bp

ATGGCGGGGCTGTACAGACGAATCCTTCCTTCTCCTCCTGCAGTCGAATTTGCTTCCTTACAGGGAAAGGAGCTTTTTCTTGAAGCCATTCAGAGTGGGACTATGGAAAACTTCTATAGGTTGATTTCTTCTTTTCAGACGCAATCAGAGCCCGCTTATTGTGGATTGGCGAGCCTGTCCATGGTTTTGAATGCCCTTGCTATTGATCCCGGAAGGAAATGGAAAGGACCCTGGAGATGGTTTGATGAATCTATGTTAGACTGCTGCGAGCCTTTGGAGACAGTTAAAGTAAGAGGGATCTCATTTGGGAAGCTTGTCTGCTTGGCTCACTGTGCTGGAGCAAAAGTTGAAGCCTTCCGCACAAATCATAGTACAATTGATGAGTTTCGTAAATATGTGTTGAGATGTTCTACATCTGATGATTCTCATGTGATCTCATCATATGATAGATCAGCTCTCAAACAGACAGGTACCGGTCATTTTTCACCAATTGGTGGATATCATGCTGGAAGAGACATGGCGCTTATTCTGGACGTTGCACGTTTTAAATATCCTCCTCATTGGGTGCCCCTCAAAATTCTTTGGGATGCCATGAATAATGTTGATCCCTCTACTGGACAACGCAGAGGGTTCATGCTTATATCTAGGCCGCACAGGGAACCAGGTCAACTTTATACCCTGAGCTGCAAACATGAGAGTTGGGCCGGTGTTGCGAAATACTTAATGGATGATGTTCCTCTTCTTCTAAAATCAGAGGAGGTGAAAGACATTCAAGAAGTTCTCTCTGTTGTTTTCATGTCGCTCCCATCCTATTTTGGGCAATTCATCAAGTGGGTTGCAGAAGTTCGGAGACGAGAAGATGGTTGCCAAAGCTTAAGCCCTGAGGAAAAAGCTAGGCTTGCTGTCAAGGGGGAGGTACTGAGACAAGTCCAGGACACTGGCCTTTTTAAACATGTGGCAGAATTATTATCTTCAGCACATTCATGCTGCGGAAACTTATATTCTGGTCACGAAGAAAACTTGCGCAACATTGCTGCGAGTGTTTGCTGCCAAGGTGCACAAATTTTGTCGGGGAAGTCTGCCTTCTCGGGGGTGTATTGTTGTCAGGAAACATGTGTGAAATGCTTGAAGGCTAATGGTGACAAGCCAGTCATGGTTGTGAGTGGGATGGTGGTAAATGGTGGAGCCGAGGAGAGGATGGACATGCTTGTTCCTTCGTCAAAAACAAATTCAGGATGTTCTTGTGCAATTGGGATGCATCCAGCTAGCAATGACATTGTAACAGCACTTCTACTGGCCTTGCCCCCAGGTACATGGTCTGGGATCAAAGACGAGAAGCTTTCGAAAGAAATATCTAACCTTGTTTCCACTGTAAATCTTCCTACTTTGCTTCAAGAAGAGGTTTTGCACTTGCGACGCCAGCTCCACCTTCTTAAGAAATGCCAAGAGGATAGGGTAGACAAAGATCTTTGCTCCCCTCTCGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006518 GO:0006725 GO:0006790 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006820 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008324 GO:0008490 GO:0008509 GO:0009056 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010035 GO:0010038 GO:0015075 GO:0015103 GO:0015105 GO:0015291 GO:0015318 GO:0015399 GO:0015405 GO:0015446 GO:0015698 GO:0015700 GO:0016043 GO:0016143 GO:0016145 GO:0016462 GO:0016740 GO:0016746 GO:0016755 GO:0016756 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019439 GO:0019748 GO:0019757 GO:0019759 GO:0019760 GO:0019762 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0033036 GO:0033037 GO:0033554 GO:0034220 GO:0034641 GO:0042221 GO:0042343 GO:0042344 GO:0042430 GO:0042436 GO:0042545 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043043 GO:0043167 GO:0043169 GO:0043207 GO:0043225 GO:0043436 GO:0043492 GO:0043603 GO:0043604 GO:0044237 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044273 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0045229 GO:0046483 GO:0046685 GO:0046686 GO:0046700 GO:0046870 GO:0046872 GO:0046914 GO:0046937 GO:0046938 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0055085 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090662 GO:0098542 GO:0098655 GO:0098656 GO:0098660 GO:0099131 GO:0099132 GO:0099133 GO:0140096 GO:1901135 GO:1901136 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901657 GO:1901658 GO:1901683 GO:1901684

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Phytochelatin Phytochelatin_C

Protein Analysis

496

Amino Acids

54.75

Weight (kDa)

7.48

Isoelectric Point (pI)

44.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Phytochelatin	PF05023	5 - 211	2.4e-88	Phytochelatin synthase
Phytochelatin_C	PF09328	229 - 475	4.9e-117	Domain of unknown function (DUF1984)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006080)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03980 AT1G03980 AT1G03980 AT1G03980 AT5G44070 AT5G44070
fragaria_vesca	FvH4_2g21060 FvH4_2g21060 FvH4_2g21060
malus_domestica	MD05G1193400.v1.1 MD10G1180000.v1.1
prunus_persica	Prupe.8G219900_v2.0.a1 Prupe.8G219900_v2.0.a1 Prupe.8G219900_v2.0.a1
pyrus_communis	pycom05g18050 pycom10g15580
rosa_chinensis	RchiOBHm_Chr6g0287511
rosa_laevigata	RLG00000012488
rosa_multiflora	Rmu_sc0007029.1_g000012
rosa_roxburghii	Rroxscaffold_7G00181410
rosa_rugosa	Rorug06G0188600
rosa_samantha	Rh6AG299900 Rh6BG305300 Rh6CG304200 Rh6CG313800 Rh6DG297200
rosa_wichuraiana	Rw6G025870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1148
Acc16I	TGCGCA	1 cut(s)	1025
Acc65I	GGTACC	1 cut(s)	470
AccB1I	GGYRCC	2 cut(s)	470, 565
AccB7I	CCANNNNNTGG	1 cut(s)	494
AccI	GTMKAC	1 cut(s)	1458
AciI	CCGC	5 cut(s)	5, 153, 358, 650, 990
AclWI	GGATC	4 cut(s)	200, 305, 599, 1331
AcsI	RAATTY	4 cut(s)	47, 576, 1062, 1228
AcuI	CTGAAG	1 cut(s)	957
AcyI	GRCGYC	1 cut(s)	1417
AfaI	GTAC	5 cut(s)	14, 373, 472, 918, 1312
AfiI	CCNNNNNNNGG	7 cut(s)	181, 237, 494, 614, 816, 1089, 1194
AflII	CTTAAG	2 cut(s)	874, 1433
AflIII	ACRYGT	2 cut(s)	952, 1112
AgeI	ACCGGT	1 cut(s)	473
AgsI	TTSAA	6 cut(s)	83, 190, 350, 773, 1129, 1394
AjnI	CCWGG	4 cut(s)	230, 661, 930, 1306
AluBI	AGCT	9 cut(s)	73, 313, 455, 684, 873, 893, 1265, 1339, 1423
AluI	AGCT	9 cut(s)	73, 313, 455, 684, 873, 893, 1265, 1339, 1423
Alw21I	GWGCWC	1 cut(s)	1060
Alw26I	GTCTC	4 cut(s)	272, 507, 844, 916
Alw44I	GTGCAC	1 cut(s)	1056
AlwI	GGATC	4 cut(s)	200, 305, 599, 1331
Ama87I	CYCGRG	1 cut(s)	1087
AoxI	GGCC	4 cut(s)	647, 702, 940, 1296
ApaLI	GTGCAC	1 cut(s)	1056
ApeKI	GCWGC	5 cut(s)	264, 684, 987, 1034, 1047
ApoI	RAATTY	4 cut(s)	47, 576, 1062, 1228
AsiGI	ACCGGT	1 cut(s)	473
Asp700I	GAANNNNTTC	1 cut(s)	87
Asp718I	GGTACC	1 cut(s)	470
AspLEI	GCGC	2 cut(s)	523, 1026
AspS9I	GGNCC	2 cut(s)	227, 702
AsuC2I	CCSGG	1 cut(s)	210
AsuHPI	GGTGA	3 cut(s)	477, 772, 1151
AsuII	TTCGAA	1 cut(s)	1343
AsuNHI	GCTAGC	1 cut(s)	1265
AvaI	CYCGRG	1 cut(s)	1087
AvaII	GGWCC	1 cut(s)	227
AxyI	CCTNAGG	1 cut(s)	882
BaeGI	GKGCMC	2 cut(s)	570, 1060
BanI	GGYRCC	2 cut(s)	470, 565
BanII	GRGCYC	1 cut(s)	153
BarI	GAAGNNNNNNTAC	2 cut(s)	1272, 1304
Bbv12I	GWGCWC	1 cut(s)	1060
BbvI	GCAGC	5 cut(s)	251, 671, 974, 1021, 1034
BccI	CCATC	5 cut(s)	231, 814, 854, 1162, 1192
BciT130I	CCWGG	4 cut(s)	232, 663, 932, 1308
BcnI	CCSGG	1 cut(s)	210
BcoDI	GTCTC	4 cut(s)	272, 507, 844, 916
BfaI	CTAG	3 cut(s)	645, 894, 1266
BfmI	CTRYAG	2 cut(s)	39, 115
BfoI	RGCGCY	1 cut(s)	524
BfrI	CTTAAG	2 cut(s)	874, 1433
BglII	AGATCT	1 cut(s)	1465
BisI	GCNGC	6 cut(s)	265, 650, 685, 988, 1035, 1048
BlsI	GCNGC	6 cut(s)	266, 651, 686, 989, 1036, 1049
Bme1390I	CCNGG	5 cut(s)	210, 232, 663, 932, 1308
Bme18I	GGWCC	1 cut(s)	227
BmeT110I	CYCGRG	1 cut(s)	1087
BmgT120I	GGNCC	2 cut(s)	227, 702
BmiI	GGNNCC	5 cut(s)	229, 472, 567, 660, 1186
BmrFI	CCNGG	5 cut(s)	210, 232, 663, 932, 1308
BmsI	GCATC	3 cut(s)	577, 1245, 1267
BmtI	GCTAGC	1 cut(s)	1269
BpmI	CTGGAG	2 cut(s)	253, 357
Bpu10I	CCTNAGC	1 cut(s)	680
Bpu14I	TTCGAA	1 cut(s)	1343
BpuMI	CCSGG	1 cut(s)	210
BsaHI	GRCGYC	1 cut(s)	1417
BsaJI	CCNNGG	5 cut(s)	180, 230, 1051, 1188, 1306
BsaWI	WCCGGW	1 cut(s)	473
BsaXI	ACNNNNNCTCC	4 cut(s)	330, 360, 1407, 1437
Bsc4I	CCNNNNNNNGG	7 cut(s)	181, 237, 494, 614, 816, 1089, 1194
Bse118I	RCCGGY	2 cut(s)	473, 704
Bse1I	ACTGG	4 cut(s)	619, 943, 1148, 1299
Bse21I	CCTNAGG	1 cut(s)	882
Bse3DI	GCAATG	2 cut(s)	1029, 1276
BseBI	CCWGG	4 cut(s)	232, 663, 932, 1308
BseDI	CCNNGG	5 cut(s)	180, 230, 1051, 1188, 1306
BseGI	GGATG	8 cut(s)	592, 733, 806, 1173, 1203, 1241, 1258, 1260
BseLI	CCNNNNNNNGG	7 cut(s)	181, 237, 494, 614, 816, 1089, 1194
BseMI	GCAATG	2 cut(s)	1029, 1276
BseMII	CTCAG	3 cut(s)	671, 873, 911
BseNI	ACTGG	4 cut(s)	619, 943, 1148, 1299
BseRI	GAGGAG	4 cut(s)	24, 546, 770, 1205
BseSI	GKGCMC	2 cut(s)	570, 1060
BseXI	GCAGC	5 cut(s)	251, 671, 974, 1021, 1034
BshFI	GGCC	4 cut(s)	649, 704, 942, 1298
BshNI	GGYRCC	2 cut(s)	470, 565
BshTI	ACCGGT	1 cut(s)	473
BsiHKAI	GWGCWC	1 cut(s)	1060
BsiHKCI	CYCGRG	1 cut(s)	1087
BsiSI	CCGG	3 cut(s)	210, 474, 705
BslFI	GGGAC	1 cut(s)	112
BslI	CCNNNNNNNGG	7 cut(s)	181, 237, 494, 614, 816, 1089, 1194
BsmAI	GTCTC	4 cut(s)	272, 507, 844, 916
BsmBI	CGTCTC	1 cut(s)	844
BsmFI	GGGAC	1 cut(s)	112
BsmI	GAATGC	1 cut(s)	196
BsnI	GGCC	4 cut(s)	649, 704, 942, 1298
BsoBI	CYCGRG	1 cut(s)	1087
Bsp119I	TTCGAA	1 cut(s)	1343
Bsp1286I	GDGCHC	3 cut(s)	153, 570, 1060
Bsp1407I	TGTACA	1 cut(s)	12
Bsp143I	GATC	7 cut(s)	205, 297, 432, 449, 604, 1323, 1465
Bsp19I	CCATGG	1 cut(s)	180
BspACI	CCGC	5 cut(s)	5, 153, 358, 650, 990
BspANI	GGCC	4 cut(s)	649, 704, 942, 1298
BspCNI	CTCAG	3 cut(s)	672, 874, 912
BspLI	GGNNCC	5 cut(s)	229, 472, 567, 660, 1186
BspMAI	CTGCAG	1 cut(s)	43
BspOI	GCTAGC	1 cut(s)	1269
BspPI	GGATC	4 cut(s)	200, 305, 599, 1331
BspT104I	TTCGAA	1 cut(s)	1343
BspT107I	GGYRCC	2 cut(s)	470, 565
BspTI	CTTAAG	2 cut(s)	874, 1433
BsrDI	GCAATG	2 cut(s)	1029, 1276
BsrFI	RCCGGY	2 cut(s)	473, 704
BsrGI	TGTACA	1 cut(s)	12
BsrI	ACTGG	4 cut(s)	619, 943, 1148, 1299
BssAI	RCCGGY	2 cut(s)	473, 704
BssECI	CCNNGG	5 cut(s)	180, 230, 1051, 1188, 1306
BssMI	GATC	7 cut(s)	205, 297, 432, 449, 604, 1323, 1465
BssNI	GRCGYC	1 cut(s)	1417
BssT1I	CCWWGG	2 cut(s)	180, 1051
Bst2UI	CCWGG	4 cut(s)	232, 663, 932, 1308
Bst4CI	ACNGT	3 cut(s)	283, 332, 1372
Bst6I	CTCTTC	3 cut(s)	505, 744, 1392
BstACI	GRCGYC	1 cut(s)	1417
BstAFI	CTTAAG	2 cut(s)	874, 1433
BstAUI	TGTACA	1 cut(s)	12
BstBI	TTCGAA	1 cut(s)	1343
BstC8I	GCNNGC	6 cut(s)	153, 172, 269, 900, 1267, 1421
BstDEI	CTNAG	4 cut(s)	680, 882, 920, 1485
BstDSI	CCRYGG	1 cut(s)	180
BstF5I	GGATG	8 cut(s)	592, 733, 806, 1173, 1203, 1241, 1258, 1260
BstH2I	RGCGCY	1 cut(s)	524
BstHHI	GCGC	2 cut(s)	523, 1026
BstKTI	GATC	7 cut(s)	208, 300, 435, 452, 607, 1326, 1468
BstMAI	GTCTC	4 cut(s)	272, 507, 844, 916
BstMBI	GATC	7 cut(s)	205, 297, 432, 449, 604, 1323, 1465
BstMWI	GCNNNNNNNGC	5 cut(s)	148, 332, 710, 899, 1420
BstNI	CCWGG	4 cut(s)	232, 663, 932, 1308
BstNSI	RCATGY	3 cut(s)	956, 1116, 1207
BstSCI	CCNGG	5 cut(s)	208, 230, 661, 930, 1306
BstSFI	CTRYAG	2 cut(s)	39, 115
BstSLI	GKGCMC	2 cut(s)	570, 1060
BstV1I	GCAGC	5 cut(s)	251, 671, 974, 1021, 1034
BstX2I	RGATCY	2 cut(s)	297, 1465
BstXI	CCANNNNNNTGG	2 cut(s)	938, 1314
BstYI	RGATCY	2 cut(s)	297, 1465
Bsu36I	CCTNAGG	1 cut(s)	882
BsuRI	GGCC	4 cut(s)	649, 704, 942, 1298
BtgI	CCRYGG	1 cut(s)	180
BtsCI	GGATG	8 cut(s)	592, 733, 806, 1173, 1203, 1241, 1258, 1260
BtsIMutI	CAGTG	3 cut(s)	328, 936, 1368
Cac8I	GCNNGC	6 cut(s)	153, 172, 269, 900, 1267, 1421
CfoI	GCGC	2 cut(s)	523, 1026
Cfr10I	RCCGGY	2 cut(s)	473, 704
Cfr13I	GGNCC	2 cut(s)	227, 702
CseI	GACGC	2 cut(s)	148, 1425
CsiI	ACCWGGT	1 cut(s)	661
Csp6I	GTAC	5 cut(s)	13, 372, 471, 917, 1311
CspAI	ACCGGT	1 cut(s)	473
CviQI	GTAC	5 cut(s)	13, 372, 471, 917, 1311
DdeI	CTNAG	4 cut(s)	680, 882, 920, 1485
DpnI	GATC	7 cut(s)	207, 299, 434, 451, 606, 1325, 1467
DpnII	GATC	7 cut(s)	205, 297, 432, 449, 604, 1323, 1465
DraI	TTTAAA	2 cut(s)	547, 949
DrdI	GACNNNNNNGTC	1 cut(s)	1148
DseDI	GACNNNNNNGTC	1 cut(s)	1148
Eam1104I	CTCTTC	3 cut(s)	505, 744, 1392
EarI	CTCTTC	3 cut(s)	505, 744, 1392
Eco130I	CCWWGG	2 cut(s)	180, 1051
Eco24I	GRGCYC	1 cut(s)	153
Eco32I	GATATC	1 cut(s)	500
Eco47I	GGWCC	1 cut(s)	227
Eco57I	CTGAAG	1 cut(s)	957
Eco81I	CCTNAGG	1 cut(s)	882
Eco88I	CYCGRG	1 cut(s)	1087
EcoO109I	RGGNCCY	1 cut(s)	227
EcoRII	CCWGG	4 cut(s)	230, 661, 930, 1306
EcoRV	GATATC	1 cut(s)	500
EcoT14I	CCWWGG	2 cut(s)	180, 1051
EcoT22I	ATGCAT	1 cut(s)	1260
EcoT38I	GRGCYC	1 cut(s)	153
ErhI	CCWWGG	2 cut(s)	180, 1051
Esp3I	CGTCTC	1 cut(s)	844
FalI	AAGNNNNNCTT	4 cut(s)	882, 914, 1067, 1099
FaqI	GGGAC	1 cut(s)	112
FauI	CCCGC	1 cut(s)	160
FauNDI	CATATG	1 cut(s)	442
FblI	GTMKAC	1 cut(s)	1458
Fnu4HI	GCNGC	6 cut(s)	265, 650, 685, 988, 1035, 1048
FokI	GGATG	8 cut(s)	599, 740, 793, 1180, 1210, 1245, 1248, 1267
FriOI	GRGCYC	1 cut(s)	153
Fsp4HI	GCNGC	6 cut(s)	265, 650, 685, 988, 1035, 1048
FspBI	CTAG	3 cut(s)	645, 894, 1266
FspI	TGCGCA	1 cut(s)	1025
GlaI	GCGC	2 cut(s)	522, 1025
GluI	GCNGC	6 cut(s)	265, 650, 685, 988, 1035, 1048
GsuI	CTGGAG	2 cut(s)	253, 357
HaeII	RGCGCY	1 cut(s)	524
HaeIII	GGCC	4 cut(s)	649, 704, 942, 1298
HapII	CCGG	3 cut(s)	210, 474, 705
HgaI	GACGC	2 cut(s)	148, 1425
HhaI	GCGC	2 cut(s)	523, 1026
Hin1I	GRCGYC	1 cut(s)	1417
Hin6I	GCGC	2 cut(s)	521, 1024
HinP1I	GCGC	2 cut(s)	521, 1024
HincII	GTYRAC	1 cut(s)	668
HindII	GTYRAC	1 cut(s)	668
HindIII	AAGCTT	3 cut(s)	311, 871, 1337
HinfI	GANTC	3 cut(s)	21, 248, 422
HpaII	CCGG	3 cut(s)	210, 474, 705
HphI	GGTGA	3 cut(s)	477, 772, 1151
Hpy166II	GTNNAC	3 cut(s)	668, 1058, 1459
Hpy188I	TCNGA	6 cut(s)	93, 138, 148, 418, 754, 849
Hpy188III	TCNNGA	7 cut(s)	80, 530, 773, 1010, 1106, 1233, 1394
Hpy8I	GTNNAC	3 cut(s)	668, 1058, 1459
Hpy99I	CGWCG	1 cut(s)	1419
HpyAV	CCTTC	8 cut(s)	35, 39, 207, 364, 1093, 1123, 1224, 1439
HpyCH4III	ACNGT	3 cut(s)	283, 332, 1372
HpyCH4IV	ACGT	2 cut(s)	534, 541
HpyCH4V	TGCA	8 cut(s)	41, 539, 687, 839, 1058, 1247, 1258, 1408
HpyF10VI	GCNNNNNNNGC	5 cut(s)	148, 332, 710, 899, 1420
HpyF3I	CTNAG	4 cut(s)	680, 882, 920, 1485
HpySE526I	ACGT	2 cut(s)	534, 541
Hsp92I	GRCGYC	1 cut(s)	1417
HspAI	GCGC	2 cut(s)	521, 1024
KpnI	GGTACC	1 cut(s)	474
Kzo9I	GATC	7 cut(s)	205, 297, 432, 449, 604, 1323, 1465
LmnI	GCTCC	6 cut(s)	70, 338, 807, 1184, 1428, 1478
Lsp1109I	GCAGC	5 cut(s)	251, 671, 974, 1021, 1034
LweI	GCATC	3 cut(s)	577, 1245, 1267
MabI	ACCWGGT	1 cut(s)	661
MaeI	CTAG	3 cut(s)	645, 894, 1266
MaeII	ACGT	2 cut(s)	534, 541
MaeIII	GTNAC	3 cut(s)	1007, 1139, 1279
MalI	GATC	7 cut(s)	207, 299, 434, 451, 606, 1325, 1467
MboI	GATC	7 cut(s)	205, 297, 432, 449, 604, 1323, 1465
MboII	GAAGA	9 cut(s)	120, 522, 731, 734, 869, 963, 1025, 1370, 1409
MfeI	CAATTG	3 cut(s)	375, 489, 1248
MflI	RGATCY	2 cut(s)	297, 1465
MhlI	GDGCHC	3 cut(s)	153, 570, 1060
MluCI	AATT	9 cut(s)	47, 375, 489, 576, 821, 962, 1062, 1228, 1248
Mph1103I	ATGCAT	1 cut(s)	1260
MroXI	GAANNNNTTC	1 cut(s)	87
MseI	TTAA	6 cut(s)	285, 546, 722, 875, 948, 1434
MslI	CAYNNNNRTG	2 cut(s)	982, 1157
MspCI	CTTAAG	2 cut(s)	874, 1433
MspI	CCGG	3 cut(s)	210, 474, 705
MspR9I	CCNGG	5 cut(s)	210, 232, 663, 932, 1308
MunI	CAATTG	3 cut(s)	375, 489, 1248
Mva1269I	GAATGC	1 cut(s)	196
MvaI	CCWGG	4 cut(s)	232, 663, 932, 1308
MwoI	GCNNNNNNNGC	5 cut(s)	148, 332, 710, 899, 1420
NciI	CCSGG	1 cut(s)	210
NcoI	CCATGG	1 cut(s)	180
NdeI	CATATG	1 cut(s)	442
NdeII	GATC	7 cut(s)	205, 297, 432, 449, 604, 1323, 1465
NheI	GCTAGC	1 cut(s)	1265
NlaIV	GGNNCC	5 cut(s)	229, 472, 567, 660, 1186
NmeAIII	GCCGAG	1 cut(s)	1213
NmuCI	GTSAC	2 cut(s)	1007, 1139
NsbI	TGCGCA	1 cut(s)	1025
NsiI	ATGCAT	1 cut(s)	1260
NspI	RCATGY	3 cut(s)	956, 1116, 1207
NspV	TTCGAA	1 cut(s)	1343
PciI	ACATGT	2 cut(s)	952, 1112
PctI	GAATGC	1 cut(s)	196
PdmI	GAANNNNTTC	1 cut(s)	87
PfeI	GAWTC	3 cut(s)	21, 248, 422
PflMI	CCANNNNNTGG	1 cut(s)	494
PfoI	TCCNGGA	2 cut(s)	208, 930
PinAI	ACCGGT	1 cut(s)	473
PkrI	GCNGC	6 cut(s)	266, 651, 686, 989, 1036, 1049
PpuMI	RGGWCCY	1 cut(s)	227
PscI	ACATGT	2 cut(s)	952, 1112
Psp5II	RGGWCCY	1 cut(s)	227
Psp6I	CCWGG	4 cut(s)	230, 661, 930, 1306
PspGI	CCWGG	4 cut(s)	230, 661, 930, 1306
PspN4I	GGNNCC	5 cut(s)	229, 472, 567, 660, 1186
PspPI	GGNCC	2 cut(s)	227, 702
PspPPI	RGGWCCY	1 cut(s)	227
PstI	CTGCAG	1 cut(s)	43
PsuI	RGATCY	2 cut(s)	297, 1465
RsaI	GTAC	5 cut(s)	14, 373, 472, 918, 1312
RsaNI	GTAC	5 cut(s)	13, 372, 471, 917, 1311
RseI	CAYNNNNRTG	2 cut(s)	982, 1157
SaqAI	TTAA	6 cut(s)	285, 546, 722, 875, 948, 1434
SatI	GCNGC	6 cut(s)	265, 650, 685, 988, 1035, 1048
Sau3AI	GATC	7 cut(s)	205, 297, 432, 449, 604, 1323, 1465
Sau96I	GGNCC	2 cut(s)	227, 702
ScrFI	CCNGG	5 cut(s)	210, 232, 663, 932, 1308
SduI	GDGCHC	3 cut(s)	153, 570, 1060
SexAI	ACCWGGT	1 cut(s)	661
SfaNI	GCATC	3 cut(s)	577, 1245, 1267
SfcI	CTRYAG	2 cut(s)	39, 115
SfuI	TTCGAA	1 cut(s)	1343
SinI	GGWCC	1 cut(s)	227
SmiMI	CAYNNNNRTG	2 cut(s)	982, 1157
SmlI	CTYRAG	2 cut(s)	874, 1433
SmoI	CTYRAG	2 cut(s)	874, 1433
Sse9I	AATT	9 cut(s)	47, 375, 489, 576, 821, 962, 1062, 1228, 1248
SsiI	CCGC	5 cut(s)	5, 153, 358, 650, 990
SspMI	CTAG	3 cut(s)	645, 894, 1266
StyD4I	CCNGG	5 cut(s)	208, 230, 661, 930, 1306
StyI	CCWWGG	2 cut(s)	180, 1051
TaaI	ACNGT	3 cut(s)	283, 332, 1372
TaiI	ACGT	2 cut(s)	537, 544
TaqI	TCGA	2 cut(s)	45, 1343
TasI	AATT	9 cut(s)	47, 375, 489, 576, 821, 962, 1062, 1228, 1248
TatI	WGTACW	2 cut(s)	12, 371
TauI	GCSGC	1 cut(s)	652
TfiI	GAWTC	3 cut(s)	21, 248, 422
Tru1I	TTAA	6 cut(s)	285, 546, 722, 875, 948, 1434
Tru9I	TTAA	6 cut(s)	285, 546, 722, 875, 948, 1434
TscAI	CASTG	3 cut(s)	335, 943, 1375
TseFI	GTSAC	2 cut(s)	1007, 1139
TseI	GCWGC	5 cut(s)	264, 684, 987, 1034, 1047
Tsp45I	GTSAC	2 cut(s)	1007, 1139
TspDTI	ATGAA	6 cut(s)	261, 608, 622, 784, 814, 972
TspRI	CASTG	3 cut(s)	335, 943, 1375
Van91I	CCANNNNNTGG	1 cut(s)	494
Vha464I	CTTAAG	2 cut(s)	874, 1433
VneI	GTGCAC	1 cut(s)	1056
VpaK11BI	GGWCC	1 cut(s)	227
XapI	RAATTY	4 cut(s)	47, 576, 1062, 1228
XceI	RCATGY	3 cut(s)	956, 1116, 1207
XmiI	GTMKAC	1 cut(s)	1458
XmnI	GAANNNNTTC	1 cut(s)	87
XspI	CTAG	3 cut(s)	645, 894, 1266
Zsp2I	ATGCAT	1 cut(s)	1260

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.