Rorug06G0188600

Glutathione gamma-glutamylcysteinyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0006080
Physical Location & Seq
Forward (+)
30127817 .. 30132701
4885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0188600.1

Sequence Viewer

Length: 1782 bp
ATGGGGTGCTTCAAACCTATCGGCTTCTTCTGCTCTGTTTTCTGCTTCGCCGTACTTGTCTGCATTTTGTTGTTGCAGTCTGAGGCTGAAGCTAGTGAGCTCTACTATGATTTTGTTCTCAAGGAAACCAATTTCACGAAGTTATGCAGCACAAAGAGTGCACTGGTTGTAAACGGAAGTTTTCCGGGGCCGGTGATTACTGCTCGCAAGGGGGATACTGTCTATGTTAATGTGCACAATGAAGGAACATATGGTGTCACCATTCACTGGCATGGGATAAAGCAACCAAGAAATCCCTGGTTTGATGGACCCGAATATATTACACAATGCCCTATTCAACCTGGAACCAATTTCACGTATACAGTGCTTCTCTCTTCGGAAGAAGGCACATTGTTTTGGCATGCCCATAGCGATTGGACCCGAGCCACCGTTCATGGAGCCTTCGTCATTTTGCCCGCAGAAAATACAACCTATCCATTTCCAGAGCCAGACCACGAAGAAGTTCTCGTTTTTGCATCATGGTATAAAGAAGATGTGGCAATATTGATGGACGAGGCTCTCAAATATGGTGGATTAACAACATTGTCGGATTCTTATGCCATCAATGGCCAGCCAGGAGATTTTTACGAATGTTCCAACGAAACAACATTTCGATTGGCGGTTGATCATGGGAGTACCTATCTTCTCCGTTTAGTGAATTCGGTACAAAACACAGACATGTTCTTCGCCATAGCAGATCATAATCTCACACTTGTGGGCGTGGATGGTAACTACGTCAAACCTGTAGTTACCAACTACCTAATGATAACCCCAGGAGAAACAATGGACATTTTGGTCACAGCAACCCAACCTCCTGGCCATTACTACATGCTTATAAGCCCTTACTTCGATGGACAAGCTGATGACTTCGACAAAAGTGTCACAAGCGCAATATTTCAGTACAACGGCAACTACACTCCTACGTCATCACCCATCTACCCAGACCACATTCCTGGCTTCTATGACATTGACGCTGCACGCAACTTTGACAGACAGTTGAAGAGTTTGGCATCTCCGGAGCACCCTGCAGATGTTCCTCAACATATCACGACCAGGATGTTTATTGCCCTTTCCATTAGCATGCTTCGTTGTCCCAACGACACATGTGATGGACCTGATGGAAATCGGCTTGCATCTGCAATGAACAACATCAGCTTTGCCAACCCTTCCATTGATGTGCTGCAAGCATACTACAGGAACATAAGTGGATATTTCGCAGCAGATTTCCCAGACAAGCCGCCTTCTCTATTCAACTTCACCTCAGATGATTTGATGACTGACAATGTTACGTTAAGTGACCAAGGAACCAGGGTGAAGATATTGAATTACAACGAGACCGTGGAAATAGTATTCCAAGGGACAAATGTCATGAACTCAGGAGAGAATCATCCCGTGCATTTGCATGGATTCAAGTTTTATGTGGTTGGAGCTGGTGTTGGGAATTTCGACAATGTCACTGATCCTTTAACTTACAATTTGATTGATCCACCAGAACTCAACACCTTCCCAGTTCCTAAGGATGGATGGGCCACCATCAGATTCGTAGCCGACAACCCAGGGGTATGGTTCATGCACTGCCATTACGATCGGCATATGAGTTGGGGCATGGATACCGCTTTCATAGTGCGGAATGGTGACACAGAGGAGACGAGTGTTCGCCCTCCACCTGATTACATGCCCTCTTGTGGCGAAAATTCCATATACGTTGGTGCTGAGCAGTCAATGTTTCAGTTGGAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006518 GO:0006725 GO:0006790 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006820 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008324 GO:0008490 GO:0008509 GO:0009056 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010035 GO:0010038 GO:0015075 GO:0015103 GO:0015105 GO:0015291 GO:0015318 GO:0015399 GO:0015405 GO:0015446 GO:0015698 GO:0015700 GO:0016043 GO:0016143 GO:0016145 GO:0016462 GO:0016740 GO:0016746 GO:0016755 GO:0016756 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019439 GO:0019748 GO:0019757 GO:0019759 GO:0019760 GO:0019762 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0033036 GO:0033037 GO:0033554 GO:0034220 GO:0034641 GO:0042221 GO:0042343 GO:0042344 GO:0042430 GO:0042436 GO:0042545 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043043 GO:0043167 GO:0043169 GO:0043207 GO:0043225 GO:0043436 GO:0043492 GO:0043603 GO:0043604 GO:0044237 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044273 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0045229 GO:0046483 GO:0046685 GO:0046686 GO:0046700 GO:0046870 GO:0046872 GO:0046914 GO:0046937 GO:0046938 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0055085 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090662 GO:0098542 GO:0098655 GO:0098656 GO:0098660 GO:0099131 GO:0099132 GO:0099133 GO:0140096 GO:1901135 GO:1901136 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901657 GO:1901658 GO:1901683 GO:1901684
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Phytochelatin Phytochelatin_C

Protein Analysis

593

Amino Acids

66.29

Weight (kDa)

4.62

Isoelectric Point (pI)

36.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 41 - 153 4.4e-39 Multicopper oxidase
Cu-oxidase PF00394 168 - 317 4.2e-37 Multicopper oxidase
Cu-oxidase_2 PF07731 421 - 558 8.9e-41 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 875
AasI GACNNNNNNGTC 2 cut(s) 833, 917
AccB7I CCANNNNNTGG 1 cut(s) 267
AccI GTMKAC 1 cut(s) 359
AccIII TCCGGA 1 cut(s) 1054
AciI CCGC 5 cut(s) 456, 659, 1277, 1653, 1666
AclWI GGATC 2 cut(s) 1493, 1517
AcoI YGGCCR 2 cut(s) 607, 856
AcsI RAATTY 3 cut(s) 697, 1480, 1732
AcuI CTGAAG 1 cut(s) 108
AfaI GTAC 4 cut(s) 54, 676, 705, 941
AfiI CCNNNNNNNGG 3 cut(s) 267, 1559, 1724
AflIII ACRYGT 2 cut(s) 717, 1142
AgsI TTSAA 6 cut(s) 13, 338, 1039, 1291, 1363, 1450
AjnI CCWGG 9 cut(s) 296, 340, 613, 811, 853, 991, 1091, 1346, 1594
AleI CACNNNNGTG 1 cut(s) 752
AluBI AGCT 5 cut(s) 92, 100, 899, 1194, 1469
AluI AGCT 5 cut(s) 92, 100, 899, 1194, 1469
Alw21I GWGCWC 4 cut(s) 102, 163, 237, 1062
Alw26I GTCTC 2 cut(s) 1367, 1679
Alw44I GTGCAC 2 cut(s) 159, 233
AlwI GGATC 2 cut(s) 1493, 1517
Ama87I CYCGRG 1 cut(s) 420
Aor13HI TCCGGA 1 cut(s) 1054
AoxI GGCC 4 cut(s) 188, 607, 856, 1566
ApaLI GTGCAC 2 cut(s) 159, 233
ApeKI GCWGC 4 cut(s) 147, 1013, 1219, 1256
ApoI RAATTY 3 cut(s) 697, 1480, 1732
ArsI GACNNNNNNTTYG 2 cut(s) 707, 739
Asp700I GAANNNNTTC 1 cut(s) 501
AspLEI GCGC 1 cut(s) 929
AspS9I GGNCC 5 cut(s) 188, 308, 417, 1151, 1566
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 6 cut(s) 205, 250, 960, 1288, 1363, 1685
AvaI CYCGRG 1 cut(s) 420
AvaII GGWCC 3 cut(s) 308, 417, 1151
AxyI CCTNAGG 1 cut(s) 1554
BaeGI GKGCMC 2 cut(s) 163, 237
BalI TGGCCA 2 cut(s) 609, 858
BanII GRGCYC 1 cut(s) 102
Bbv12I GWGCWC 4 cut(s) 102, 163, 237, 1062
BbvI GCAGC 4 cut(s) 159, 1000, 1206, 1268
BceAI ACGGC 2 cut(s) 35, 961
BciT130I CCWGG 9 cut(s) 298, 342, 615, 813, 855, 993, 1093, 1348, 1596
BciVI GTATCC 2 cut(s) 208, 1642
BclI TGATCA 1 cut(s) 664
BcnI CCSGG 1 cut(s) 186
BcoDI GTCTC 2 cut(s) 1367, 1679
BfaI CTAG 1 cut(s) 93
BfmI CTRYAG 3 cut(s) 783, 1065, 1231
BfuI GTATCC 2 cut(s) 208, 1642
BisI GCNGC 5 cut(s) 148, 1014, 1220, 1257, 1277
BlpI GCTNAGC 1 cut(s) 1752
BlsI GCNGC 5 cut(s) 149, 1015, 1221, 1258, 1278
Bme18I GGWCC 3 cut(s) 308, 417, 1151
BmeT110I CYCGRG 1 cut(s) 420
BmgT120I GGNCC 5 cut(s) 188, 308, 417, 1151, 1566
BmiI GGNNCC 6 cut(s) 189, 310, 346, 419, 439, 1345
BmrI ACTGGG 1 cut(s) 1541
BmsI GCATC 3 cut(s) 524, 1058, 1181
BmuI ACTGGG 1 cut(s) 1541
BoxI GACNNNNGTC 1 cut(s) 1403
Bpu1102I GCTNAGC 1 cut(s) 1752
BpuEI CTTGAG 1 cut(s) 104
BpuMI CCSGG 1 cut(s) 186
BsaAI YACGTR 1 cut(s) 357
BsaBI GATNNNNATC 2 cut(s) 741, 1161
BsaI GGTCTC 1 cut(s) 1367
BsaJI CCNNGG 9 cut(s) 185, 296, 811, 1339, 1347, 1377, 1393, 1594, 1595
BsaWI WCCGGW 1 cut(s) 1054
BsaXI ACNNNNNCTCC 6 cut(s) 429, 459, 835, 865, 1458, 1488
Bsc4I CCNNNNNNNGG 3 cut(s) 267, 1559, 1724
Bse118I RCCGGY 1 cut(s) 190
Bse1I ACTGG 3 cut(s) 168, 272, 1547
Bse21I CCTNAGG 1 cut(s) 1554
Bse3DI GCAATG 1 cut(s) 1185
Bse8I GATNNNNATC 2 cut(s) 741, 1161
BseAI TCCGGA 1 cut(s) 1054
BseBI CCWGG 9 cut(s) 298, 342, 615, 813, 855, 993, 1093, 1348, 1596
BseDI CCNNGG 9 cut(s) 185, 296, 811, 1339, 1347, 1377, 1393, 1594, 1595
BseGI GGATG 5 cut(s) 769, 1101, 1426, 1564, 1568
BseJI GATNNNNATC 2 cut(s) 741, 1161
BseLI CCNNNNNNNGG 3 cut(s) 267, 1559, 1724
BseMI GCAATG 1 cut(s) 1185
BseMII CTCAG 4 cut(s) 72, 1314, 1428, 1743
BseNI ACTGG 3 cut(s) 168, 272, 1547
BseRI GAGGAG 1 cut(s) 1697
BseSI GKGCMC 2 cut(s) 163, 237
BseXI GCAGC 4 cut(s) 159, 1000, 1206, 1268
BsgI GTGCAG 1 cut(s) 999
Bsh1285I CGRYCG 1 cut(s) 1627
BshFI GGCC 4 cut(s) 190, 609, 858, 1568
BsiEI CGRYCG 1 cut(s) 1627
BsiHKAI GWGCWC 4 cut(s) 102, 163, 237, 1062
BsiHKCI CYCGRG 1 cut(s) 420
BsiSI CCGG 3 cut(s) 185, 191, 1055
BslFI GGGAC 2 cut(s) 1116, 1411
BslI CCNNNNNNNGG 3 cut(s) 267, 1559, 1724
BsmAI GTCTC 2 cut(s) 1367, 1679
BsmBI CGTCTC 1 cut(s) 1679
BsmFI GGGAC 2 cut(s) 1116, 1411
BsnI GGCC 4 cut(s) 190, 609, 858, 1568
Bso31I GGTCTC 1 cut(s) 1367
BsoBI CYCGRG 1 cut(s) 420
Bsp1286I GDGCHC 4 cut(s) 102, 163, 237, 1062
Bsp13I TCCGGA 1 cut(s) 1054
Bsp143I GATC 5 cut(s) 664, 736, 1498, 1522, 1624
Bsp1720I GCTNAGC 1 cut(s) 1752
BspACI CCGC 5 cut(s) 456, 659, 1277, 1653, 1666
BspANI GGCC 4 cut(s) 190, 609, 858, 1568
BspCNI CTCAG 4 cut(s) 73, 1313, 1427, 1744
BspEI TCCGGA 1 cut(s) 1054
BspHI TCATGA 1 cut(s) 1407
BspLI GGNNCC 6 cut(s) 189, 310, 346, 419, 439, 1345
BspMAI CTGCAG 1 cut(s) 1069
BspPI GGATC 2 cut(s) 1493, 1517
BspTNI GGTCTC 1 cut(s) 1367
BsrDI GCAATG 1 cut(s) 1185
BsrFI RCCGGY 1 cut(s) 190
BsrI ACTGG 3 cut(s) 168, 272, 1547
BssAI RCCGGY 1 cut(s) 190
BssECI CCNNGG 9 cut(s) 185, 296, 811, 1339, 1347, 1377, 1393, 1594, 1595
BssMI GATC 5 cut(s) 664, 736, 1498, 1522, 1624
BssNAI GTATAC 1 cut(s) 360
BssT1I CCWWGG 2 cut(s) 1339, 1393
Bst1107I GTATAC 1 cut(s) 360
Bst2UI CCWGG 9 cut(s) 298, 342, 615, 813, 855, 993, 1093, 1348, 1596
Bst4CI ACNGT 5 cut(s) 220, 364, 430, 1035, 1378
Bst6I CTCTTC 2 cut(s) 379, 1034
BstBAI YACGTR 1 cut(s) 357
BstC8I GCNNGC 8 cut(s) 205, 402, 456, 611, 1018, 1121, 1170, 1224
BstDEI CTNAG 5 cut(s) 81, 1300, 1414, 1554, 1752
BstDSI CCRYGG 1 cut(s) 1377
BstF5I GGATG 5 cut(s) 769, 1101, 1426, 1564, 1568
BstHHI GCGC 1 cut(s) 929
BstKTI GATC 5 cut(s) 667, 739, 1501, 1525, 1627
BstMAI GTCTC 2 cut(s) 1367, 1679
BstMBI GATC 5 cut(s) 664, 736, 1498, 1522, 1624
BstMCI CGRYCG 1 cut(s) 1627
BstMWI GCNNNNNNNGC 1 cut(s) 30
BstNI CCWGG 9 cut(s) 298, 342, 615, 813, 855, 993, 1093, 1348, 1596
BstNSI RCATGY 6 cut(s) 404, 721, 871, 1123, 1146, 1717
BstPAI GACNNNNGTC 1 cut(s) 1403
BstSFI CTRYAG 3 cut(s) 783, 1065, 1231
BstSLI GKGCMC 2 cut(s) 163, 237
BstV1I GCAGC 4 cut(s) 159, 1000, 1206, 1268
BstXI CCANNNNNNTGG 3 cut(s) 854, 992, 1602
BstZ17I GTATAC 1 cut(s) 360
Bsu36I CCTNAGG 1 cut(s) 1554
BsuI GTATCC 2 cut(s) 208, 1642
BsuRI GGCC 4 cut(s) 190, 609, 858, 1568
BtgI CCRYGG 1 cut(s) 1377
BtsCI GGATG 5 cut(s) 769, 1101, 1426, 1564, 1568
BtsI GCAGTG 1 cut(s) 1612
BtsIMutI CAGTG 5 cut(s) 161, 265, 369, 1494, 1612
Cac8I GCNNGC 8 cut(s) 205, 402, 456, 611, 1018, 1121, 1170, 1224
CciI TCATGA 1 cut(s) 1407
CfoI GCGC 1 cut(s) 929
Cfr10I RCCGGY 1 cut(s) 190
Cfr13I GGNCC 5 cut(s) 188, 308, 417, 1151, 1566
CseI GACGC 1 cut(s) 1019
Csp6I GTAC 4 cut(s) 53, 675, 704, 940
CspCI CAANNNNNGTGG 2 cut(s) 550, 585
CviQI GTAC 4 cut(s) 53, 675, 704, 940
DdeI CTNAG 5 cut(s) 81, 1300, 1414, 1554, 1752
DpnI GATC 5 cut(s) 666, 738, 1500, 1524, 1626
DpnII GATC 5 cut(s) 664, 736, 1498, 1522, 1624
DrdI GACNNNNNNGTC 2 cut(s) 833, 917
DseDI GACNNNNNNGTC 2 cut(s) 833, 917
EaeI YGGCCR 2 cut(s) 607, 856
Eam1104I CTCTTC 2 cut(s) 379, 1034
EarI CTCTTC 2 cut(s) 379, 1034
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 2 cut(s) 1339, 1393
Eco24I GRGCYC 1 cut(s) 102
Eco31I GGTCTC 1 cut(s) 1367
Eco47I GGWCC 3 cut(s) 308, 417, 1151
Eco53kI GAGCTC 1 cut(s) 100
Eco57I CTGAAG 1 cut(s) 108
Eco81I CCTNAGG 1 cut(s) 1554
Eco88I CYCGRG 1 cut(s) 420
EcoICRI GAGCTC 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 697
EcoRII CCWGG 9 cut(s) 296, 340, 613, 811, 853, 991, 1091, 1346, 1594
EcoT14I CCWWGG 2 cut(s) 1339, 1393
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 2 cut(s) 1339, 1393
Esp3I CGTCTC 1 cut(s) 1679
FaqI GGGAC 2 cut(s) 1116, 1411
FauI CCCGC 1 cut(s) 463
FauNDI CATATG 2 cut(s) 250, 1632
FbaI TGATCA 1 cut(s) 664
FblI GTMKAC 1 cut(s) 359
Fnu4HI GCNGC 5 cut(s) 148, 1014, 1220, 1257, 1277
FokI GGATG 5 cut(s) 776, 1108, 1413, 1571, 1575
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 5 cut(s) 148, 1014, 1220, 1257, 1277
FspBI CTAG 1 cut(s) 93
GlaI GCGC 1 cut(s) 928
GluI GCNGC 5 cut(s) 148, 1014, 1220, 1257, 1277
HaeIII GGCC 4 cut(s) 190, 609, 858, 1568
HapII CCGG 3 cut(s) 185, 191, 1055
HgaI GACGC 1 cut(s) 1019
HhaI GCGC 1 cut(s) 929
Hin6I GCGC 1 cut(s) 927
HinP1I GCGC 1 cut(s) 927
HinfI GANTC 4 cut(s) 590, 1423, 1446, 1578
HpaII CCGG 3 cut(s) 185, 191, 1055
HphI GGTGA 6 cut(s) 205, 250, 960, 1288, 1363, 1685
Hpy166II GTNNAC 4 cut(s) 161, 172, 235, 360
Hpy188I TCNGA 5 cut(s) 82, 379, 589, 1303, 1577
Hpy188III TCNNGA 6 cut(s) 136, 482, 1055, 1087, 1408, 1416
Hpy8I GTNNAC 4 cut(s) 161, 172, 235, 360
HpyAV CCTTC 6 cut(s) 236, 377, 451, 1215, 1290, 1552
HpyCH4III ACNGT 5 cut(s) 220, 364, 430, 1035, 1378
HpyCH4IV ACGT 5 cut(s) 356, 774, 962, 1328, 1743
HpyF10VI GCNNNNNNNGC 1 cut(s) 30
HpyF3I CTNAG 5 cut(s) 81, 1300, 1414, 1554, 1752
HpySE526I ACGT 5 cut(s) 356, 774, 962, 1328, 1743
HspAI GCGC 1 cut(s) 927
Kpn2I TCCGGA 1 cut(s) 1054
Ksp22I TGATCA 1 cut(s) 664
Kzo9I GATC 5 cut(s) 664, 736, 1498, 1522, 1624
LmnI GCTCC 3 cut(s) 437, 1057, 1466
Lsp1109I GCAGC 4 cut(s) 159, 1000, 1206, 1268
LweI GCATC 3 cut(s) 524, 1058, 1181
MaeI CTAG 1 cut(s) 93
MaeII ACGT 5 cut(s) 356, 774, 962, 1328, 1743
MaeIII GTNAC 9 cut(s) 256, 767, 787, 835, 919, 1324, 1334, 1492, 1673
MalI GATC 5 cut(s) 666, 738, 1500, 1524, 1626
MboI GATC 5 cut(s) 664, 736, 1498, 1522, 1624
MboII GAAGA 9 cut(s) 19, 366, 392, 509, 542, 674, 715, 1051, 1366
MhlI GDGCHC 4 cut(s) 102, 163, 237, 1062
MlsI TGGCCA 2 cut(s) 609, 858
MluCI AATT 7 cut(s) 130, 349, 697, 1363, 1480, 1513, 1732
MluNI TGGCCA 2 cut(s) 609, 858
MmeI TCCRAC 4 cut(s) 567, 660, 1444, 1752
MnlI CCTC 9 cut(s) 76, 547, 861, 1086, 1309, 1675, 1710, 1729, 1768
Mox20I TGGCCA 2 cut(s) 609, 858
MroI TCCGGA 1 cut(s) 1054
MroXI GAANNNNTTC 1 cut(s) 501
MscI TGGCCA 2 cut(s) 609, 858
MseI TTAA 4 cut(s) 228, 575, 1331, 1505
MslI CAYNNNNRTG 6 cut(s) 270, 716, 752, 1118, 1214, 1440
Msp20I TGGCCA 2 cut(s) 609, 858
MspI CCGG 3 cut(s) 185, 191, 1055
MvaI CCWGG 9 cut(s) 298, 342, 615, 813, 855, 993, 1093, 1348, 1596
MwoI GCNNNNNNNGC 1 cut(s) 30
NciI CCSGG 1 cut(s) 186
NdeI CATATG 2 cut(s) 250, 1632
NdeII GATC 5 cut(s) 664, 736, 1498, 1522, 1624
NlaIV GGNNCC 6 cut(s) 189, 310, 346, 419, 439, 1345
NmuCI GTSAC 6 cut(s) 256, 835, 919, 1334, 1492, 1673
NspI RCATGY 6 cut(s) 404, 721, 871, 1123, 1146, 1717
OliI CACNNNNGTG 1 cut(s) 752
PaeI GCATGC 2 cut(s) 404, 1123
PagI TCATGA 1 cut(s) 1407
PasI CCCWGGG 1 cut(s) 1595
PciI ACATGT 2 cut(s) 717, 1142
PdmI GAANNNNTTC 1 cut(s) 501
PfeI GAWTC 4 cut(s) 590, 1423, 1446, 1578
PflFI GACNNNGTC 1 cut(s) 1490
PflMI CCANNNNNTGG 1 cut(s) 267
PkrI GCNGC 5 cut(s) 149, 1015, 1221, 1258, 1278
Ple19I CGATCG 1 cut(s) 1627
Ppu21I YACGTR 1 cut(s) 357
PscI ACATGT 2 cut(s) 717, 1142
PshAI GACNNNNGTC 1 cut(s) 1403
PsiI TTATAA 1 cut(s) 875
Psp124BI GAGCTC 1 cut(s) 102
Psp6I CCWGG 9 cut(s) 296, 340, 613, 811, 853, 991, 1091, 1346, 1594
PspGI CCWGG 9 cut(s) 296, 340, 613, 811, 853, 991, 1091, 1346, 1594
PspN4I GGNNCC 6 cut(s) 189, 310, 346, 419, 439, 1345
PspPI GGNCC 5 cut(s) 188, 308, 417, 1151, 1566
PstI CTGCAG 1 cut(s) 1069
PsyI GACNNNGTC 1 cut(s) 1490
PvuI CGATCG 1 cut(s) 1627
RsaI GTAC 4 cut(s) 54, 676, 705, 941
RsaNI GTAC 4 cut(s) 53, 675, 704, 940
RseI CAYNNNNRTG 6 cut(s) 270, 716, 752, 1118, 1214, 1440
SacI GAGCTC 1 cut(s) 102
SaqAI TTAA 4 cut(s) 228, 575, 1331, 1505
SatI GCNGC 5 cut(s) 148, 1014, 1220, 1257, 1277
Sau3AI GATC 5 cut(s) 664, 736, 1498, 1522, 1624
Sau96I GGNCC 5 cut(s) 188, 308, 417, 1151, 1566
SduI GDGCHC 4 cut(s) 102, 163, 237, 1062
SfaNI GCATC 3 cut(s) 524, 1058, 1181
SfcI CTRYAG 3 cut(s) 783, 1065, 1231
SinI GGWCC 3 cut(s) 308, 417, 1151
SmiMI CAYNNNNRTG 6 cut(s) 270, 716, 752, 1118, 1214, 1440
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
SphI GCATGC 2 cut(s) 404, 1123
Sse9I AATT 7 cut(s) 130, 349, 697, 1363, 1480, 1513, 1732
SsiI CCGC 5 cut(s) 456, 659, 1277, 1653, 1666
SspI AATATT 2 cut(s) 543, 933
SspMI CTAG 1 cut(s) 93
SstI GAGCTC 1 cut(s) 102
StyI CCWWGG 2 cut(s) 1339, 1393
TaaI ACNGT 5 cut(s) 220, 364, 430, 1035, 1378
TaiI ACGT 5 cut(s) 359, 777, 965, 1331, 1746
TaqI TCGA 4 cut(s) 652, 888, 909, 1485
TasI AATT 7 cut(s) 130, 349, 697, 1363, 1480, 1513, 1732
TatI WGTACW 1 cut(s) 939
TauI GCSGC 1 cut(s) 1279
TfiI GAWTC 4 cut(s) 590, 1423, 1446, 1578
Tru1I TTAA 4 cut(s) 228, 575, 1331, 1505
Tru9I TTAA 4 cut(s) 228, 575, 1331, 1505
TscAI CASTG 5 cut(s) 168, 272, 369, 1501, 1619
TseFI GTSAC 6 cut(s) 256, 835, 919, 1334, 1492, 1673
TseI GCWGC 4 cut(s) 147, 1013, 1219, 1256
Tsp45I GTSAC 6 cut(s) 256, 835, 919, 1334, 1492, 1673
TspDTI ATGAA 6 cut(s) 255, 422, 1196, 1424, 1597, 1648
TspGWI ACGGA 2 cut(s) 189, 677
TspRI CASTG 5 cut(s) 168, 272, 369, 1501, 1619
Tth111I GACNNNGTC 1 cut(s) 1490
Van91I CCANNNNNTGG 1 cut(s) 267
VneI GTGCAC 2 cut(s) 159, 233
VpaK11BI GGWCC 3 cut(s) 308, 417, 1151
XapI RAATTY 3 cut(s) 697, 1480, 1732
XceI RCATGY 6 cut(s) 404, 721, 871, 1123, 1146, 1717
XcmI CCANNNNNNNNNTGG 1 cut(s) 294
XmiI GTMKAC 1 cut(s) 359
XmnI GAANNNNTTC 1 cut(s) 501
XspI CTAG 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.