AT1G09100

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0019131
Physical Location & Seq
Reverse (-)
2936528 .. 2939363
2836 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G09100.1

Sequence Viewer

Length: 1272 bp
ATGGCAACGGCGATGGCGGAGGATACGAGCTTCGAGGGAGACCAACTAGCTTCCATGACTACTGATGACATCGGTAGAGCTTCTCGTCTCTTAGCCAACGAGATTCGCATCCTCAAGGAAGAATCGCAGAGGACAAACCTTGATTTGGAATCAGTGAAGGAGAAAATAAAGGAGAACCAGGAGAAGATTAAGCTTAACAAACAGCTTCCTTACTTAGTTGGCAATATCGTTGAGATTCTTGAGATGAGTCCAGAGGATGATGCAGAGGAAGATGGAGCGAATATCGATCTGGACTCTCAGAGGAAGGGAAAGTGTGTCGTTCTAAAAACATCAACTCGTCAGACCATCTTCCTCCCTGTTGTTGGACTTGTTGATCCAGATACATTGAAGCCTGGGGACTTAGTTGGAGTTAACAAAGATAGTTACTTGATTCTGGATACTTTACCTTCTGAGTATGATTCAAGAGTAAAAGCAATGGAGGTCGACGAAAAACCTACTGAAGACTACAATGATATAGGGGGACTAGAAAAACAGATACAAGAACTTGTTGAAGCCATTGTGTTGCCAATGACTCATAAAGAGCAGTTCGAAAAGTTGGGTATCCGTCCACCAAAAGGTGTACTCTTGTATGGTCCTCCTGGAACCGGAAAGACGTTGATGGCCCGAGCTTGTGCAGCACAGACAAATGCAACTTTCCTCAAACTAGCAGGGCCACAACTTGTTCAGATGTTCATCGGAGATGGAGCAAAACTTGTCCGTGATGCTTTCCTGCTTGCAAAAGAGAAATCCCCTTGTATTATATTCATAGATGAGATTGACGCAATAGGGACAAAGCGTTTTGATAGCGAAGTTAGTGGAGACCGTGAGGTACAAAGGACAATGTTGGAGCTGCTCAACCAGCTTGATGGATTCAGCAGCGATGATCGCATTAAGGTTATTGCAGCGACAAATCGTGCTGATATACTAGATCCTGCCCTTATGCGTTCTGGTCGTTTGGATCGTAAAATCGAGTTTCCGCACCCCACTGAAGAAGCTAGAGGCAGGATCTTACAGATACATTCAAGGAAAATGAATGTGAATGCAGACGTCAATTTTGAAGAGCTTGCTCGGTCAACGGATGATTTCAATGGAGCTCAACTCAAAGCAGTGTGTGTTGAAGCGGGTATGCTTGCCCTGCGCCGGGATGCAACTGAGGTGAACCATGAGGATTTCAATGAAGGTATAATTCAAGTACAAGCCAAGAAGAAAGCAAGCTTGAATTACTACGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0001678 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005838 GO:0005886 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008540 GO:0009056 GO:0009057 GO:0009553 GO:0009555 GO:0009743 GO:0009746 GO:0009749 GO:0009756 GO:0009757 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010182 GO:0010243 GO:0010255 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019725 GO:0019941 GO:0022624 GO:0023052 GO:0030162 GO:0030163 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032991 GO:0033500 GO:0033554 GO:0034284 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042592 GO:0042593 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048229 GO:0048518 GO:0048522 GO:0048856 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0055082 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0065008 GO:0070013 GO:0070887 GO:0071310 GO:0071322 GO:0071326 GO:0071331 GO:0071333 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901700 GO:1901701 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

423

Amino Acids

47.04

Weight (kDa)

4.9

Isoelectric Point (pI)

39.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_ATP_ID_OB_C PF16450 73 - 149 7.8e-10 Proteasomal ATPase OB C-terminal domain
AAA_5 PF07728 206 - 326 2.1e-07 AAA domain (dynein-related subfamily)
AAA PF00004 207 - 339 3.8e-43 ATPase family associated with various cellular activities (AAA)
nSTAND3 PF20720 207 - 353 7.6e-06 Novel STAND NTPase 3
AAA_lid_3 PF17862 362 - 406 5.2e-15 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019131)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09100
malus_domestica MD04G1199700.v1.1 MD04G1199800.v1.1 MD12G1212500.v1.1
pyrus_communis pycom04g17640
rosa_samantha Rh2CG288900 Rh5BG388700 Rh6BG234600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1089
AccI GTMKAC 1 cut(s) 483
AciI CCGC 3 cut(s) 17, 1016, 1160
AclWI GGATC 4 cut(s) 368, 962, 1005, 1052
AcuI CTGAAG 2 cut(s) 519, 1047
AcyI GRCGYC 1 cut(s) 1086
AfaI GTAC 3 cut(s) 621, 870, 1233
AfiI CCNNNNNNNGG 6 cut(s) 145, 362, 614, 644, 1179, 1180
AjnI CCWGG 3 cut(s) 177, 391, 637
Alw21I GWGCWC 1 cut(s) 1135
Alw26I GTCTC 3 cut(s) 33, 92, 852
AlwI GGATC 4 cut(s) 368, 962, 1005, 1052
Ama87I CYCGRG 1 cut(s) 663
AoxI GGCC 2 cut(s) 660, 710
ApeKI GCWGC 4 cut(s) 674, 889, 915, 941
ArsI GACNNNNNNTTYG 2 cut(s) 1076, 1108
AspLEI GCGC 1 cut(s) 1179
AspS9I GGNCC 3 cut(s) 632, 661, 710
AsuC2I CCSGG 1 cut(s) 1181
AsuHPI GGTGA 1 cut(s) 1207
AsuII TTCGAA 1 cut(s) 588
AvaI CYCGRG 1 cut(s) 663
AvaII GGWCC 1 cut(s) 632
BanII GRGCYC 1 cut(s) 1135
BbsI GAAGAC 1 cut(s) 507
Bbv12I GWGCWC 1 cut(s) 1135
BbvI GCAGC 4 cut(s) 686, 876, 927, 953
BccI CCATC 6 cut(s) 7, 266, 353, 652, 734, 899
BceAI ACGGC 1 cut(s) 24
BciT130I CCWGG 3 cut(s) 179, 393, 639
BciVI GTATCC 3 cut(s) 16, 430, 611
BcnI CCSGG 1 cut(s) 1181
BcoDI GTCTC 3 cut(s) 33, 92, 852
BfaI CTAG 5 cut(s) 47, 524, 704, 965, 1035
BfuI GTATCC 3 cut(s) 16, 430, 611
BisI GCNGC 4 cut(s) 675, 890, 916, 942
BlsI GCNGC 4 cut(s) 676, 891, 917, 943
Bme1390I CCNGG 4 cut(s) 179, 393, 639, 1181
Bme18I GGWCC 1 cut(s) 632
BmeT110I CYCGRG 1 cut(s) 663
BmgT120I GGNCC 3 cut(s) 632, 661, 710
BmiI GGNNCC 1 cut(s) 643
BmrFI CCNGG 4 cut(s) 179, 393, 639, 1181
BmsI GCATC 4 cut(s) 117, 250, 751, 1174
BpiI GAAGAC 1 cut(s) 507
BplI GAGNNNNNCTC 2 cut(s) 1122, 1154
Bpu14I TTCGAA 1 cut(s) 588
BpuEI CTTGAG 2 cut(s) 98, 260
BpuMI CCSGG 1 cut(s) 1181
Bsa29I ATCGAT 1 cut(s) 285
BsaBI GATNNNNATC 2 cut(s) 107, 731
BsaHI GRCGYC 1 cut(s) 1086
BsaI GGTCTC 2 cut(s) 33, 852
BsaJI CCNNGG 1 cut(s) 392
BsaWI WCCGGW 1 cut(s) 644
Bsc4I CCNNNNNNNGG 6 cut(s) 145, 362, 614, 644, 1179, 1180
Bse3DI GCAATG 1 cut(s) 480
Bse8I GATNNNNATC 2 cut(s) 107, 731
BseBI CCWGG 3 cut(s) 179, 393, 639
BseCI ATCGAT 1 cut(s) 285
BseDI CCNNGG 1 cut(s) 392
BseGI GGATG 4 cut(s) 108, 262, 1123, 1189
BseJI GATNNNNATC 2 cut(s) 107, 731
BseLI CCNNNNNNNGG 6 cut(s) 145, 362, 614, 644, 1179, 1180
BseMI GCAATG 1 cut(s) 480
BseMII CTCAG 3 cut(s) 311, 441, 1182
BseXI GCAGC 4 cut(s) 686, 876, 927, 953
BsgI GTGCAG 1 cut(s) 693
BshFI GGCC 2 cut(s) 662, 712
BshVI ATCGAT 1 cut(s) 285
BsiHKAI GWGCWC 1 cut(s) 1135
BsiHKCI CYCGRG 1 cut(s) 663
BsiSI CCGG 2 cut(s) 645, 1180
BslFI GGGAC 3 cut(s) 410, 534, 841
BslI CCNNNNNNNGG 6 cut(s) 145, 362, 614, 644, 1179, 1180
BsmAI GTCTC 3 cut(s) 33, 92, 852
BsmBI CGTCTC 1 cut(s) 92
BsmFI GGGAC 3 cut(s) 410, 534, 841
BsmI GAATGC 1 cut(s) 1084
BsnI GGCC 2 cut(s) 662, 712
Bso31I GGTCTC 2 cut(s) 33, 852
BsoBI CYCGRG 1 cut(s) 663
Bsp119I TTCGAA 1 cut(s) 588
Bsp1286I GDGCHC 1 cut(s) 1135
Bsp143I GATC 6 cut(s) 286, 373, 922, 967, 997, 1044
BspACI CCGC 3 cut(s) 17, 1016, 1160
BspANI GGCC 2 cut(s) 662, 712
BspCNI CTCAG 3 cut(s) 310, 442, 1183
BspDI ATCGAT 1 cut(s) 285
BspLI GGNNCC 1 cut(s) 643
BspPI GGATC 4 cut(s) 368, 962, 1005, 1052
BspQI GCTCTTC 1 cut(s) 1092
BspT104I TTCGAA 1 cut(s) 588
BspTNI GGTCTC 2 cut(s) 33, 852
BsrDI GCAATG 1 cut(s) 480
BssECI CCNNGG 1 cut(s) 392
BssMI GATC 6 cut(s) 286, 373, 922, 967, 997, 1044
BssNI GRCGYC 1 cut(s) 1086
Bst2UI CCWGG 3 cut(s) 179, 393, 639
Bst4CI ACNGT 1 cut(s) 863
Bst6I CTCTTC 1 cut(s) 1092
BstACI GRCGYC 1 cut(s) 1086
BstBI TTCGAA 1 cut(s) 588
BstC8I GCNNGC 4 cut(s) 774, 1104, 1170, 1252
BstDEI CTNAG 6 cut(s) 91, 214, 297, 400, 450, 1191
BstF5I GGATG 4 cut(s) 108, 262, 1123, 1189
BstHHI GCGC 1 cut(s) 1179
BstKTI GATC 6 cut(s) 289, 376, 925, 970, 1000, 1047
BstMAI GTCTC 3 cut(s) 33, 92, 852
BstMBI GATC 6 cut(s) 286, 373, 922, 967, 997, 1044
BstMWI GCNNNNNNNGC 4 cut(s) 674, 898, 924, 1174
BstNI CCWGG 3 cut(s) 179, 393, 639
BstSCI CCNGG 4 cut(s) 177, 391, 637, 1179
BstV1I GCAGC 4 cut(s) 686, 876, 927, 953
BstV2I GAAGAC 1 cut(s) 507
BstX2I RGATCY 2 cut(s) 967, 1044
BstXI CCANNNNNNTGG 1 cut(s) 905
BstYI RGATCY 2 cut(s) 967, 1044
Bsu15I ATCGAT 1 cut(s) 285
BsuI GTATCC 3 cut(s) 16, 430, 611
BsuRI GGCC 2 cut(s) 662, 712
BsuTUI ATCGAT 1 cut(s) 285
BtgZI GCGATG 2 cut(s) 26, 933
BtsCI GGATG 4 cut(s) 108, 262, 1123, 1189
BtsI GCAGTG 1 cut(s) 1152
BtsIMutI CAGTG 3 cut(s) 159, 1023, 1152
Cac8I GCNNGC 4 cut(s) 774, 1104, 1170, 1252
CfoI GCGC 1 cut(s) 1179
Cfr13I GGNCC 3 cut(s) 632, 661, 710
ClaI ATCGAT 1 cut(s) 285
CseI GACGC 1 cut(s) 827
Csp6I GTAC 3 cut(s) 620, 869, 1232
CviAII CATG 2 cut(s) 55, 1202
CviQI GTAC 3 cut(s) 620, 869, 1232
DdeI CTNAG 6 cut(s) 91, 214, 297, 400, 450, 1191
DpnI GATC 6 cut(s) 288, 375, 924, 969, 999, 1046
DpnII GATC 6 cut(s) 286, 373, 922, 967, 997, 1044
Eam1104I CTCTTC 1 cut(s) 1092
EarI CTCTTC 1 cut(s) 1092
EciI GGCGGA 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 1133
Eco24I GRGCYC 1 cut(s) 1135
Eco31I GGTCTC 2 cut(s) 33, 852
Eco47I GGWCC 1 cut(s) 632
Eco53kI GAGCTC 1 cut(s) 1133
Eco57I CTGAAG 2 cut(s) 519, 1047
Eco88I CYCGRG 1 cut(s) 663
EcoICRI GAGCTC 1 cut(s) 1133
EcoRII CCWGG 3 cut(s) 177, 391, 637
EcoT38I GRGCYC 1 cut(s) 1135
Esp3I CGTCTC 1 cut(s) 92
FaeI CATG 2 cut(s) 58, 1205
FaqI GGGAC 3 cut(s) 410, 534, 841
FatI CATG 2 cut(s) 54, 1201
FauI CCCGC 1 cut(s) 1153
FblI GTMKAC 1 cut(s) 483
Fnu4HI GCNGC 4 cut(s) 675, 890, 916, 942
FokI GGATG 4 cut(s) 95, 269, 1130, 1196
FriOI GRGCYC 1 cut(s) 1135
Fsp4HI GCNGC 4 cut(s) 675, 890, 916, 942
FspBI CTAG 5 cut(s) 47, 524, 704, 965, 1035
GlaI GCGC 1 cut(s) 1178
GluI GCNGC 4 cut(s) 675, 890, 916, 942
HaeIII GGCC 2 cut(s) 662, 712
HapII CCGG 2 cut(s) 645, 1180
HgaI GACGC 1 cut(s) 827
HhaI GCGC 1 cut(s) 1179
Hin1I GRCGYC 1 cut(s) 1086
Hin1II CATG 2 cut(s) 58, 1205
Hin6I GCGC 1 cut(s) 1177
HinP1I GCGC 1 cut(s) 1177
HincII GTYRAC 3 cut(s) 412, 484, 1113
HindII GTYRAC 3 cut(s) 412, 484, 1113
HindIII AAGCTT 2 cut(s) 191, 1252
HpaI GTTAAC 1 cut(s) 412
HpaII CCGG 2 cut(s) 645, 1180
HphI GGTGA 1 cut(s) 1207
Hpy166II GTNNAC 6 cut(s) 412, 484, 608, 620, 1113, 1198
Hpy188I TCNGA 5 cut(s) 300, 342, 451, 726, 737
Hpy188III TCNNGA 6 cut(s) 239, 251, 290, 377, 434, 462
Hpy8I GTNNAC 6 cut(s) 412, 484, 608, 620, 1113, 1198
Hpy99I CGWCG 1 cut(s) 488
HpyAV CCTTC 4 cut(s) 151, 298, 456, 1211
HpyCH4III ACNGT 1 cut(s) 863
HpyCH4IV ACGT 2 cut(s) 653, 1086
HpyCH4V TGCA 7 cut(s) 263, 674, 689, 776, 941, 1082, 1187
HpyF10VI GCNNNNNNNGC 4 cut(s) 674, 898, 924, 1174
HpyF3I CTNAG 6 cut(s) 91, 214, 297, 400, 450, 1191
HpySE526I ACGT 2 cut(s) 653, 1086
Hsp92I GRCGYC 1 cut(s) 1086
Hsp92II CATG 2 cut(s) 58, 1205
HspAI GCGC 1 cut(s) 1177
KspAI GTTAAC 1 cut(s) 412
Kzo9I GATC 6 cut(s) 286, 373, 922, 967, 997, 1044
LguI GCTCTTC 1 cut(s) 1092
LmnI GCTCC 4 cut(s) 275, 743, 886, 1130
Lsp1109I GCAGC 4 cut(s) 686, 876, 927, 953
LweI GCATC 4 cut(s) 117, 250, 751, 1174
MaeI CTAG 5 cut(s) 47, 524, 704, 965, 1035
MaeII ACGT 2 cut(s) 653, 1086
MaeIII GTNAC 1 cut(s) 422
MalI GATC 6 cut(s) 288, 375, 924, 969, 999, 1046
MboI GATC 6 cut(s) 286, 373, 922, 967, 997, 1044
MboII GAAGA 8 cut(s) 131, 196, 281, 340, 512, 1040, 1109, 1255
MflI RGATCY 2 cut(s) 967, 1044
MhlI GDGCHC 1 cut(s) 1135
MluCI AATT 3 cut(s) 1090, 1224, 1258
MlyI GAGTC 3 cut(s) 256, 287, 565
MmeI TCCRAC 3 cut(s) 343, 385, 864
MseI TTAA 4 cut(s) 189, 195, 411, 930
MspI CCGG 2 cut(s) 645, 1180
MspR9I CCNGG 4 cut(s) 179, 393, 639, 1181
Mva1269I GAATGC 1 cut(s) 1084
MvaI CCWGG 3 cut(s) 179, 393, 639
MwoI GCNNNNNNNGC 4 cut(s) 674, 898, 924, 1174
NciI CCSGG 1 cut(s) 1181
NdeII GATC 6 cut(s) 286, 373, 922, 967, 997, 1044
NlaIII CATG 2 cut(s) 58, 1205
NlaIV GGNNCC 1 cut(s) 643
NspV TTCGAA 1 cut(s) 588
PciSI GCTCTTC 1 cut(s) 1092
PcsI WCGNNNNNNNCGW 1 cut(s) 997
PctI GAATGC 1 cut(s) 1084
PfeI GAWTC 7 cut(s) 103, 122, 149, 235, 430, 458, 909
PfoI TCCNGGA 1 cut(s) 637
PkrI GCNGC 4 cut(s) 676, 891, 917, 943
PleI GAGTC 3 cut(s) 255, 287, 565
PpsI GAGTC 3 cut(s) 255, 287, 565
Psp124BI GAGCTC 1 cut(s) 1135
Psp6I CCWGG 3 cut(s) 177, 391, 637
PspGI CCWGG 3 cut(s) 177, 391, 637
PspN4I GGNNCC 1 cut(s) 643
PspPI GGNCC 3 cut(s) 632, 661, 710
PsuI RGATCY 2 cut(s) 967, 1044
RsaI GTAC 3 cut(s) 621, 870, 1233
RsaNI GTAC 3 cut(s) 620, 869, 1232
SacI GAGCTC 1 cut(s) 1135
SalI GTCGAC 1 cut(s) 482
SapI GCTCTTC 1 cut(s) 1092
SaqAI TTAA 4 cut(s) 189, 195, 411, 930
SatI GCNGC 4 cut(s) 675, 890, 916, 942
Sau3AI GATC 6 cut(s) 286, 373, 922, 967, 997, 1044
Sau96I GGNCC 3 cut(s) 632, 661, 710
SchI GAGTC 3 cut(s) 256, 287, 565
ScrFI CCNGG 4 cut(s) 179, 393, 639, 1181
SduI GDGCHC 1 cut(s) 1135
SfaNI GCATC 4 cut(s) 117, 250, 751, 1174
SfuI TTCGAA 1 cut(s) 588
SinI GGWCC 1 cut(s) 632
SmlI CTYRAG 2 cut(s) 113, 239
SmoI CTYRAG 2 cut(s) 113, 239
Sse9I AATT 3 cut(s) 1090, 1224, 1258
SsiI CCGC 3 cut(s) 17, 1016, 1160
SspMI CTAG 5 cut(s) 47, 524, 704, 965, 1035
SstI GAGCTC 1 cut(s) 1135
StyD4I CCNGG 4 cut(s) 177, 391, 637, 1179
TaaI ACNGT 1 cut(s) 863
TaiI ACGT 2 cut(s) 656, 1089
TaqI TCGA 5 cut(s) 33, 285, 483, 588, 1008
TaqII GACCGA 1 cut(s) 1098
TasI AATT 3 cut(s) 1090, 1224, 1258
TatI WGTACW 2 cut(s) 619, 1231
TfiI GAWTC 7 cut(s) 103, 122, 149, 235, 430, 458, 909
Tru1I TTAA 4 cut(s) 189, 195, 411, 930
Tru9I TTAA 4 cut(s) 189, 195, 411, 930
TscAI CASTG 3 cut(s) 159, 1030, 1152
TseI GCWGC 4 cut(s) 674, 889, 915, 941
TspDTI ATGAA 4 cut(s) 721, 793, 1085, 1230
TspGWI ACGGA 3 cut(s) 593, 746, 1130
TspRI CASTG 3 cut(s) 159, 1030, 1152
VpaK11BI GGWCC 1 cut(s) 632
XmiI GTMKAC 1 cut(s) 483
XspI CTAG 5 cut(s) 47, 524, 704, 965, 1035
ZraI GACGTC 1 cut(s) 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.