MD12G1212500.v1.1

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0019131
Physical Location & Seq
Reverse (-)
29146837 .. 29150465
3629 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1212500.v1.1.491

Sequence Viewer

Length: 1272 bp
ATGGCGAACGTCATGGTAGAAGACACGACCTTTGAAGATGATCAGCTCGCCGCGATGACCATCGAAGACATCGTCAGAGCCACTCGTCTTCTTGACAACAAGATCCGAATACTCAAGGAAGAAATGTCAAGAACGAATTTGGAGCTGGACTCGTACAAGGACAAGATTAAGGAGAATCAGGAAAAGATTAAGCTCAATAAGCAGTTGCCTTACTTGGTGGGCAACATTGTTGAGATTCTTGAAATGAACCCAGAAGATGAGGCCGAGGAGGATGGTGCAAATATTGATCTTGACTCCCAAAGAAAGGGAAAATGTGTTGTGTTAAAGACATCTACTCGCCAGACAATCTTTCTGCCAGTTGTTGGGCTTGTTGATCCTGATAACTTGAAACCTGGGGATCTTGTTGGTGTGAACAAAGATAGTTACCTGATCTTGGATACTCTACCGTCTGAGTATGATTCTCGGGTGAAGGCTATGGAGGTTGATGAAAAGCCAACTGAAGACTACAATGACATTGGAGGGCTGGAGAAGCAGATTCAAGAACTAGTTGAGGCGATTGTTTTGCCCATGACCCACAAGGAGCGTTTTCAGAAATTGGGGGTTCGTCCACCAAAGGGAGTGCTACTGTATGGACCTCCGGGAACTGGTAAAACTTTGATGGCCCGGGCTTGTGCTGCGCAGACAAATGCCACTTTTCTGAAACTAGCTGGTCCACAACTGGTTCAGATGTTCATTGGGGATGGAGCAAAACTTGTTCGTGATGCCTTTCAGCTCGCAAAAGAGAAATCCCCCTGCATCATTTTCATAGATGAAATTGATGCAATCGGCACAAAGCGGTTTGATAGTGAAGTGAGTGGAGATAGGGAGGTGCAGCGTACAATGCTTGAGTTGCTTAATCAGCTTGATGGCTTTAGTAGCGATGATCGAATTAAGGTGATAGCAGCAACCAATCGAGCTGACATCCTCGACCCTGCTCTTATGCGTTCTGGTAGGTTGGATCGAAAAATTGAGTTTCCACATCCCACTGAAGAAGCAAGAGCTCGAATTTTGCAGATTCACTCAAGAAAGATGAATGTTCATCCGGATGTCAATTTCGAAGAATTAGCTCGCTCAACCGATGACTTCAACGGAGCACAGCTAAAAGCTGTATGTGTGGAGGCAGGCATGCTTGCCCTTCGTCGTGATGCCACAGAGGTGAACCACGAGGACTTTAACGAGGGCATTATCCAAGTTCAGGCAAAGAAAAAAGCCAGCCTCAATTATTATGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005838 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008134 GO:0008150 GO:0008152 GO:0008540 GO:0009056 GO:0009057 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0022624 GO:0030162 GO:0030163 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033554 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

424

Amino Acids

47.49

Weight (kDa)

5.01

Isoelectric Point (pI)

39.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_ATP_ID_OB_C PF16450 73 - 149 1.2e-09 Proteasomal ATPase OB C-terminal domain
AAA_5 PF07728 206 - 326 1.9e-06 AAA domain (dynein-related subfamily)
AAA PF00004 207 - 339 4.9e-44 ATPase family associated with various cellular activities (AAA)
nSTAND3 PF20720 207 - 354 6.4e-07 Novel STAND NTPase 3
AAA_lid_3 PF17862 362 - 406 5.2e-15 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019131)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09100
malus_domestica MD04G1199700.v1.1 MD04G1199800.v1.1 MD12G1212500.v1.1
pyrus_communis pycom04g17640
rosa_samantha Rh2CG288900 Rh5BG388700 Rh6BG234600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 678
AccB7I CCANNNNNTGG 1 cut(s) 362
AccII CGCG 1 cut(s) 53
AccIII TCCGGA 1 cut(s) 1081
AciI CCGC 2 cut(s) 51, 835
AclWI GGATC 4 cut(s) 97, 368, 405, 1005
AcsI RAATTY 2 cut(s) 136, 1044
AcuI CTGAAG 2 cut(s) 519, 1047
AfaI GTAC 2 cut(s) 155, 877
AfiI CCNNNNNNNGG 6 cut(s) 304, 362, 433, 614, 644, 1234
AgsI TTSAA 5 cut(s) 35, 242, 388, 539, 1126
AhlI ACTAGT 1 cut(s) 544
AjnI CCWGG 1 cut(s) 391
AleI CACNNNNGTG 1 cut(s) 1193
Alw21I GWGCWC 2 cut(s) 1042, 1135
AlwI GGATC 4 cut(s) 97, 368, 405, 1005
Ama87I CYCGRG 2 cut(s) 462, 663
Aor13HI TCCGGA 1 cut(s) 1081
AoxI GGCC 2 cut(s) 261, 660
ApeKI GCWGC 3 cut(s) 674, 871, 941
ApoI RAATTY 2 cut(s) 136, 1044
ArsI GACNNNNNNTTYG 4 cut(s) 14, 46, 57, 89
AspLEI GCGC 1 cut(s) 679
AspS9I GGNCC 3 cut(s) 632, 661, 710
AsuC2I CCSGG 3 cut(s) 639, 664, 665
AsuHPI GGTGA 3 cut(s) 478, 946, 1207
AsuII TTCGAA 1 cut(s) 1095
AvaI CYCGRG 2 cut(s) 462, 663
AvaII GGWCC 2 cut(s) 632, 710
BanII GRGCYC 1 cut(s) 1042
BauI CACGAG 1 cut(s) 1202
BbsI GAAGAC 4 cut(s) 27, 72, 80, 507
Bbv12I GWGCWC 2 cut(s) 1042, 1135
BbvI GCAGC 3 cut(s) 661, 883, 953
BccI CCATC 5 cut(s) 68, 266, 652, 734, 899
BcgI CGANNNNNNTGC 2 cut(s) 544, 578
BciT130I CCWGG 1 cut(s) 393
BciVI GTATCC 1 cut(s) 430
BclI TGATCA 1 cut(s) 40
BcnI CCSGG 3 cut(s) 639, 664, 665
BcuI ACTAGT 1 cut(s) 544
BfaI CTAG 3 cut(s) 545, 704, 1270
BfuI GTATCC 1 cut(s) 430
BisI GCNGC 4 cut(s) 51, 675, 872, 942
BlsI GCNGC 4 cut(s) 52, 676, 873, 943
Bme1390I CCNGG 4 cut(s) 393, 639, 664, 665
Bme18I GGWCC 2 cut(s) 632, 710
BmeT110I CYCGRG 2 cut(s) 462, 663
BmgT120I GGNCC 3 cut(s) 632, 661, 710
BmrFI CCNGG 4 cut(s) 393, 639, 664, 665
BmsI GCATC 4 cut(s) 751, 804, 808, 1174
BpiI GAAGAC 4 cut(s) 27, 72, 80, 507
BplI GAGNNNNNCTC 2 cut(s) 134, 166
BpmI CTGGAG 1 cut(s) 545
Bpu14I TTCGAA 1 cut(s) 1095
BpuEI CTTGAG 3 cut(s) 98, 905, 1045
BpuMI CCSGG 3 cut(s) 639, 664, 665
BsaBI GATNNNNATC 1 cut(s) 59
BsaJI CCNNGG 3 cut(s) 264, 392, 663
BsaWI WCCGGW 1 cut(s) 1081
Bsc4I CCNNNNNNNGG 6 cut(s) 304, 362, 433, 614, 644, 1234
Bse1I ACTGG 3 cut(s) 356, 649, 723
Bse8I GATNNNNATC 1 cut(s) 59
BseAI TCCGGA 1 cut(s) 1081
BseBI CCWGG 1 cut(s) 393
BseDI CCNNGG 3 cut(s) 264, 392, 663
BseGI GGATG 6 cut(s) 277, 745, 960, 1018, 1078, 1090
BseJI GATNNNNATC 1 cut(s) 59
BseLI CCNNNNNNNGG 6 cut(s) 304, 362, 433, 614, 644, 1234
BseMII CTCAG 1 cut(s) 441
BseNI ACTGG 3 cut(s) 356, 649, 723
BseRI GAGGAG 1 cut(s) 281
BseXI GCAGC 3 cut(s) 661, 883, 953
BsgI GTGCAG 1 cut(s) 890
Bsh1236I CGCG 1 cut(s) 53
BshFI GGCC 2 cut(s) 263, 662
BsiHKAI GWGCWC 2 cut(s) 1042, 1135
BsiHKCI CYCGRG 2 cut(s) 462, 663
BsiSI CCGG 3 cut(s) 638, 664, 1082
BslI CCNNNNNNNGG 6 cut(s) 304, 362, 433, 614, 644, 1234
BsnI GGCC 2 cut(s) 263, 662
BsoBI CYCGRG 2 cut(s) 462, 663
Bsp119I TTCGAA 1 cut(s) 1095
Bsp1286I GDGCHC 2 cut(s) 1042, 1135
Bsp13I TCCGGA 1 cut(s) 1081
Bsp143I GATC 8 cut(s) 40, 102, 286, 373, 397, 429, 922, 997
BspACI CCGC 2 cut(s) 51, 835
BspANI GGCC 2 cut(s) 263, 662
BspCNI CTCAG 1 cut(s) 442
BspEI TCCGGA 1 cut(s) 1081
BspFNI CGCG 1 cut(s) 53
BspPI GGATC 4 cut(s) 97, 368, 405, 1005
BspT104I TTCGAA 1 cut(s) 1095
BsrI ACTGG 3 cut(s) 356, 649, 723
BssECI CCNNGG 3 cut(s) 264, 392, 663
BssMI GATC 8 cut(s) 40, 102, 286, 373, 397, 429, 922, 997
BssSI CACGAG 1 cut(s) 1202
Bst2BI CACGAG 1 cut(s) 1202
Bst2UI CCWGG 1 cut(s) 393
Bst4CI ACNGT 2 cut(s) 447, 627
BstBI TTCGAA 1 cut(s) 1095
BstC8I GCNNGC 7 cut(s) 48, 774, 1108, 1162, 1166, 1170, 1252
BstDEI CTNAG 1 cut(s) 450
BstF5I GGATG 6 cut(s) 277, 745, 960, 1018, 1078, 1090
BstFNI CGCG 1 cut(s) 53
BstHHI GCGC 1 cut(s) 679
BstKTI GATC 8 cut(s) 43, 105, 289, 376, 400, 432, 925, 1000
BstMBI GATC 8 cut(s) 40, 102, 286, 373, 397, 429, 922, 997
BstMWI GCNNNNNNNGC 7 cut(s) 199, 529, 674, 880, 889, 898, 915
BstNI CCWGG 1 cut(s) 393
BstNSI RCATGY 1 cut(s) 1168
BstSCI CCNGG 4 cut(s) 391, 637, 662, 663
BstUI CGCG 1 cut(s) 53
BstV1I GCAGC 3 cut(s) 661, 883, 953
BstV2I GAAGAC 4 cut(s) 27, 72, 80, 507
BstX2I RGATCY 2 cut(s) 102, 397
BstYI RGATCY 2 cut(s) 102, 397
BsuI GTATCC 1 cut(s) 430
BsuRI GGCC 2 cut(s) 263, 662
BtgZI GCGATG 2 cut(s) 68, 933
BtsCI GGATG 6 cut(s) 277, 745, 960, 1018, 1078, 1090
BtsIMutI CAGTG 1 cut(s) 1023
Cac8I GCNNGC 7 cut(s) 48, 774, 1108, 1162, 1166, 1170, 1252
CfoI GCGC 1 cut(s) 679
Cfr13I GGNCC 3 cut(s) 632, 661, 710
Cfr9I CCCGGG 1 cut(s) 663
Csp6I GTAC 2 cut(s) 154, 876
CviAII CATG 3 cut(s) 13, 568, 1165
CviQI GTAC 2 cut(s) 154, 876
DdeI CTNAG 1 cut(s) 450
DpnI GATC 8 cut(s) 42, 104, 288, 375, 399, 431, 924, 999
DpnII GATC 8 cut(s) 40, 102, 286, 373, 397, 429, 922, 997
Ecl136II GAGCTC 1 cut(s) 1040
Eco24I GRGCYC 1 cut(s) 1042
Eco47I GGWCC 2 cut(s) 632, 710
Eco53kI GAGCTC 1 cut(s) 1040
Eco57I CTGAAG 2 cut(s) 519, 1047
Eco88I CYCGRG 2 cut(s) 462, 663
EcoICRI GAGCTC 1 cut(s) 1040
EcoRII CCWGG 1 cut(s) 391
EcoT38I GRGCYC 1 cut(s) 1042
FaeI CATG 3 cut(s) 16, 571, 1168
FatI CATG 3 cut(s) 12, 567, 1164
FbaI TGATCA 1 cut(s) 40
Fnu4HI GCNGC 4 cut(s) 51, 675, 872, 942
FokI GGATG 6 cut(s) 284, 752, 947, 1005, 1065, 1097
FriOI GRGCYC 1 cut(s) 1042
Fsp4HI GCNGC 4 cut(s) 51, 675, 872, 942
FspBI CTAG 3 cut(s) 545, 704, 1270
FspI TGCGCA 1 cut(s) 678
GlaI GCGC 1 cut(s) 678
GluI GCNGC 4 cut(s) 51, 675, 872, 942
GsuI CTGGAG 1 cut(s) 545
HaeIII GGCC 2 cut(s) 263, 662
HapII CCGG 3 cut(s) 638, 664, 1082
HhaI GCGC 1 cut(s) 679
Hin1II CATG 3 cut(s) 16, 571, 1168
Hin6I GCGC 1 cut(s) 677
HinP1I GCGC 1 cut(s) 677
HinfI GANTC 7 cut(s) 149, 175, 235, 293, 458, 535, 1054
HpaII CCGG 3 cut(s) 638, 664, 1082
HphI GGTGA 3 cut(s) 478, 946, 1207
Hpy166II GTNNAC 4 cut(s) 412, 608, 713, 1198
Hpy188I TCNGA 6 cut(s) 77, 107, 451, 591, 699, 726
Hpy8I GTNNAC 4 cut(s) 412, 608, 713, 1198
Hpy99I CGWCG 1 cut(s) 1182
HpyAV CCTTC 2 cut(s) 463, 1184
HpyCH4III ACNGT 2 cut(s) 447, 627
HpyCH4IV ACGT 1 cut(s) 9
HpyCH4V TGCA 5 cut(s) 278, 795, 821, 871, 1051
HpyF10VI GCNNNNNNNGC 7 cut(s) 199, 529, 674, 880, 889, 898, 915
HpyF3I CTNAG 1 cut(s) 450
HpySE526I ACGT 1 cut(s) 9
Hsp92II CATG 3 cut(s) 16, 571, 1168
HspAI GCGC 1 cut(s) 677
Kpn2I TCCGGA 1 cut(s) 1081
Ksp22I TGATCA 1 cut(s) 40
Kzo9I GATC 8 cut(s) 40, 102, 286, 373, 397, 429, 922, 997
LmnI GCTCC 4 cut(s) 142, 580, 743, 1130
Lsp1109I GCAGC 3 cut(s) 661, 883, 953
LweI GCATC 4 cut(s) 751, 804, 808, 1174
MaeI CTAG 3 cut(s) 545, 704, 1270
MaeII ACGT 1 cut(s) 9
MaeIII GTNAC 1 cut(s) 422
MalI GATC 8 cut(s) 42, 104, 288, 375, 399, 431, 924, 999
MboI GATC 8 cut(s) 40, 102, 286, 373, 397, 429, 922, 997
MboII GAAGA 9 cut(s) 32, 47, 77, 80, 131, 266, 512, 1040, 1109
MflI RGATCY 2 cut(s) 102, 397
MhlI GDGCHC 2 cut(s) 1042, 1135
MluCI AATT 9 cut(s) 136, 593, 813, 927, 1005, 1044, 1090, 1100, 1258
MlyI GAGTC 2 cut(s) 143, 287
MmeI TCCRAC 1 cut(s) 975
MroI TCCGGA 1 cut(s) 1081
MseI TTAA 6 cut(s) 168, 189, 323, 894, 930, 1212
MslI CAYNNNNRTG 2 cut(s) 1083, 1193
MspI CCGG 3 cut(s) 638, 664, 1082
MspR9I CCNGG 4 cut(s) 393, 639, 664, 665
MvaI CCWGG 1 cut(s) 393
MvnI CGCG 1 cut(s) 53
MwoI GCNNNNNNNGC 7 cut(s) 199, 529, 674, 880, 889, 898, 915
NciI CCSGG 3 cut(s) 639, 664, 665
NdeII GATC 8 cut(s) 40, 102, 286, 373, 397, 429, 922, 997
NlaIII CATG 3 cut(s) 16, 571, 1168
NmeAIII GCCGAG 1 cut(s) 289
NsbI TGCGCA 1 cut(s) 678
NspI RCATGY 1 cut(s) 1168
NspV TTCGAA 1 cut(s) 1095
OliI CACNNNNGTG 1 cut(s) 1193
PaeI GCATGC 1 cut(s) 1168
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PfeI GAWTC 5 cut(s) 175, 235, 458, 535, 1054
PflFI GACNNNGTC 1 cut(s) 71
PflMI CCANNNNNTGG 1 cut(s) 362
PfoI TCCNGGA 1 cut(s) 637
PkrI GCNGC 4 cut(s) 52, 676, 873, 943
PleI GAGTC 2 cut(s) 143, 287
PpsI GAGTC 2 cut(s) 143, 287
Psp124BI GAGCTC 1 cut(s) 1042
Psp6I CCWGG 1 cut(s) 391
PspGI CCWGG 1 cut(s) 391
PspPI GGNCC 3 cut(s) 632, 661, 710
PsuI RGATCY 2 cut(s) 102, 397
PsyI GACNNNGTC 1 cut(s) 71
RsaI GTAC 2 cut(s) 155, 877
RsaNI GTAC 2 cut(s) 154, 876
RseI CAYNNNNRTG 2 cut(s) 1083, 1193
SacI GAGCTC 1 cut(s) 1042
SaqAI TTAA 6 cut(s) 168, 189, 323, 894, 930, 1212
SatI GCNGC 4 cut(s) 51, 675, 872, 942
Sau3AI GATC 8 cut(s) 40, 102, 286, 373, 397, 429, 922, 997
Sau96I GGNCC 3 cut(s) 632, 661, 710
SchI GAGTC 2 cut(s) 143, 287
ScrFI CCNGG 4 cut(s) 393, 639, 664, 665
SduI GDGCHC 2 cut(s) 1042, 1135
SfaNI GCATC 4 cut(s) 751, 804, 808, 1174
SfuI TTCGAA 1 cut(s) 1095
SinI GGWCC 2 cut(s) 632, 710
SmaI CCCGGG 1 cut(s) 665
SmiMI CAYNNNNRTG 2 cut(s) 1083, 1193
SmlI CTYRAG 3 cut(s) 113, 884, 1060
SmoI CTYRAG 3 cut(s) 113, 884, 1060
SpeI ACTAGT 1 cut(s) 544
SphI GCATGC 1 cut(s) 1168
SrfI GCCCGGGC 1 cut(s) 665
Sse9I AATT 9 cut(s) 136, 593, 813, 927, 1005, 1044, 1090, 1100, 1258
SsiI CCGC 2 cut(s) 51, 835
SspI AATATT 1 cut(s) 283
SspMI CTAG 3 cut(s) 545, 704, 1270
SstI GAGCTC 1 cut(s) 1042
StyD4I CCNGG 4 cut(s) 391, 637, 662, 663
TaaI ACNGT 2 cut(s) 447, 627
TaiI ACGT 1 cut(s) 12
TaqI TCGA 7 cut(s) 63, 925, 952, 966, 1000, 1042, 1095
TasI AATT 9 cut(s) 136, 593, 813, 927, 1005, 1044, 1090, 1100, 1258
TauI GCSGC 1 cut(s) 53
TfiI GAWTC 5 cut(s) 175, 235, 458, 535, 1054
Tru1I TTAA 6 cut(s) 168, 189, 323, 894, 930, 1212
Tru9I TTAA 6 cut(s) 168, 189, 323, 894, 930, 1212
TscAI CASTG 1 cut(s) 1030
TseI GCWGC 3 cut(s) 674, 871, 941
TspDTI ATGAA 7 cut(s) 260, 501, 721, 793, 825, 1067, 1085
TspGWI ACGGA 1 cut(s) 1143
TspMI CCCGGG 1 cut(s) 663
TspRI CASTG 1 cut(s) 1030
Tth111I GACNNNGTC 1 cut(s) 71
Van91I CCANNNNNTGG 1 cut(s) 362
VpaK11BI GGWCC 2 cut(s) 632, 710
XapI RAATTY 2 cut(s) 136, 1044
XceI RCATGY 1 cut(s) 1168
XmaI CCCGGG 1 cut(s) 663
XspI CTAG 3 cut(s) 545, 704, 1270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.