AT1G14330

kelch repeat-containing F-box family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006468
Physical Location & Seq
Reverse (-)
4890069 .. 4892332
2264 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G14330.1

Sequence Viewer

Length: 1326 bp
ATGGTGGAAGATCGGACATATTTGATGTCAAGGATTTTCTCAAGTTCTCGTCTTTCAGAATCAAAGTGGCCATACATGTATCCACAACCAGAAGATTCCTCTGAATCTAATCTCATCAATGGCAAAAGAGCATTGGAGAATGATGTTGATGAGCTCAGACAGAGCAAGTCTCCCAGATTGATGGGATTTTCAATTCATGGGAATGAAGCCATTGAAGAAGATGAACAAGAACAAGACCAATCTGATTCTAACAACAATGGTAATAGCGATGGCGATTCACTTATCAATGATATTGGCAGAGACAATTCTATAAGCTGTTTGATTCGTTGCTCGAGGTCTGGTTACGGTTCGATTGCTTCTTTAAACCGGAGTTTTCGGTCTTTGGTGAAGACCGGAGAGATTTATAGACTCAGGAGGCAAAACCAGATTGTTGAACATTGGGTTTACTTCTCTTGTCAGCTTTTGGAATGGGTTGCTTTCAATCCTTTTGAAAGAAGATGGATGAATTTGCCTACAATGCCTTCAGGTGTTACATTCATGTGTGCCGATAAAGAATCTTTAGCTGTTGGTACGGATTTGCTTGTGTTAGGCAAAGATGATTACTCTTCTCATGTTATATATAGATATAGTCTTTTGACTAACTCTTGGTCTTCCGGTATGAGGATGAATTCTCCGAGGTGTTTGTTTGGCTCAGCGAGTCTAGGAGAGATTGCGATCTTTGCTGGTGGTTTTGATTCTTTTGGCAAAATCAGTGATTCCGCTGAGATGTATAACTCCGAGCTTCAGACTTGGACTACACTTCCGAAGATGAATAAACCAAGGAAGATGTGTTCAGGTGTTTTCATGGATGGGAAGTTCTATGTGATTGGAGGAATTGGTGGAAATGATTCGAAAGTGCTTACTTGTGGTGAGGAGTTTGATTTAGAGACGAAGAAATGGACTGAGATACCGGAAATGTCGCCTCCAAGGAGCCGGGAAATGCCGGCAGCAGCAGAGGCGCCGCCTCTTGTTGCGGTTGTGAATAACGAATTGTATGCTGCTGATCATGCTGATATGGAAGTGAGGAAGTATGATAAGGAGAGTAAGAAATGGTTTACTTTAGGGAGATTGCCCGAAAGAGCTGACTCGGTTAACGGTTGGGGATTAGCGTTTAGAGCTTGCGGTGAGAGGTTGATTGTGATTGGTGGTCCTAGGAGTTCAGGTGGAGGGTACATTGAACTGAATTCATGGATACCATCTAGTGATCGAAGTCCGCCTCTATGGACATTGCTCGGTAGGAAACATTCATCGAATTTTGTTTACAATTGCGCGGTGATGGGTTGCTGA

Protein Analysis

441

Amino Acids

49.52

Weight (kDa)

5.42

Isoelectric Point (pI)

59.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ATRN-LZTR1 PF24981 144 - 321 6.3e-10 Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 160 - 322 9e-09 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_1 PF01344 225 - 271 1e-07 Kelch motif
Beta-prop_Calicin PF13964 237 - 377 6.2e-10 Calicin, beta-propeller domain
Kelch_FKB95 PF25210 248 - 320 4.9e-10 FKB95, Kelch-repeats domain
Kelch_1 PF01344 273 - 320 1.4e-11 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 997
AccII CGCG 1 cut(s) 1310
AciI CCGC 6 cut(s) 759, 1001, 1013, 1161, 1253, 1310
AcoI YGGCCR 1 cut(s) 68
AcsI RAATTY 4 cut(s) 505, 667, 1222, 1291
AcuI CTGAAG 2 cut(s) 507, 767
AcyI GRCGYC 1 cut(s) 998
AfaI GTAC 2 cut(s) 571, 1211
AfiI CCNNNNNNNGG 1 cut(s) 660
AflIII ACRYGT 1 cut(s) 75
AgsI TTSAA 6 cut(s) 192, 215, 434, 481, 491, 1217
AloI GAACNNNNNNTCC 4 cut(s) 814, 839, 846, 871
AluBI AGCT 7 cut(s) 154, 315, 460, 563, 781, 1121, 1157
AluI AGCT 7 cut(s) 154, 315, 460, 563, 781, 1121, 1157
Alw21I GWGCWC 1 cut(s) 156
Alw26I GTCTC 3 cut(s) 174, 294, 920
Ama87I CYCGRG 1 cut(s) 331
AoxI GGCC 1 cut(s) 68
ApeKI GCWGC 3 cut(s) 986, 989, 1037
ApoI RAATTY 4 cut(s) 505, 667, 1222, 1291
Asp700I GAANNNNTTC 1 cut(s) 886
AspA2I CCTAGG 1 cut(s) 1190
AspLEI GCGC 2 cut(s) 1000, 1310
AspS9I GGNCC 1 cut(s) 1187
AsuC2I CCSGG 1 cut(s) 974
AsuHPI GGTGA 4 cut(s) 397, 920, 1175, 1324
AsuII TTCGAA 1 cut(s) 890
AvaI CYCGRG 1 cut(s) 331
AvaII GGWCC 1 cut(s) 1187
AvrII CCTAGG 1 cut(s) 1190
BalI TGGCCA 1 cut(s) 70
BanI GGYRCC 1 cut(s) 997
BanII GRGCYC 1 cut(s) 156
BbsI GAAGAC 2 cut(s) 395, 642
Bbv12I GWGCWC 1 cut(s) 156
BbvI GCAGC 3 cut(s) 998, 1001, 1024
BccI CCATC 6 cut(s) 175, 263, 492, 842, 1243, 1309
BcgI CGANNNNNNTGC 2 cut(s) 1016, 1050
BciVI GTATCC 2 cut(s) 90, 1224
BclI TGATCA 1 cut(s) 1042
BcnI CCSGG 1 cut(s) 974
BcoDI GTCTC 3 cut(s) 174, 294, 920
BfaI CTAG 3 cut(s) 701, 1191, 1239
BfoI RGCGCY 1 cut(s) 1001
BfuI GTATCC 2 cut(s) 90, 1224
BisI GCNGC 4 cut(s) 987, 990, 1001, 1038
BlnI CCTAGG 1 cut(s) 1190
BlpI GCTNAGC 1 cut(s) 691
BlsI GCNGC 4 cut(s) 988, 991, 1002, 1039
Bme1390I CCNGG 1 cut(s) 974
Bme18I GGWCC 1 cut(s) 1187
BmeT110I CYCGRG 1 cut(s) 331
BmgT120I GGNCC 1 cut(s) 1187
BmiI GGNNCC 2 cut(s) 971, 999
BmrFI CCNGG 1 cut(s) 974
BpiI GAAGAC 2 cut(s) 395, 642
BplI GAGNNNNNCTC 2 cut(s) 154, 186
Bpu1102I GCTNAGC 1 cut(s) 691
Bpu14I TTCGAA 1 cut(s) 890
BpuEI CTTGAG 1 cut(s) 25
BpuMI CCSGG 1 cut(s) 974
BsaBI GATNNNNATC 1 cut(s) 713
BsaHI GRCGYC 1 cut(s) 998
BsaJI CCNNGG 4 cut(s) 674, 818, 965, 1190
BsaWI WCCGGW 4 cut(s) 366, 392, 653, 949
BsaXI ACNNNNNCTCC 2 cut(s) 1186, 1216
Bsc4I CCNNNNNNNGG 1 cut(s) 660
Bse118I RCCGGY 1 cut(s) 982
Bse3DI GCAATG 1 cut(s) 1265
Bse8I GATNNNNATC 1 cut(s) 713
BseDI CCNNGG 4 cut(s) 674, 818, 965, 1190
BseGI GGATG 3 cut(s) 507, 669, 853
BseJI GATNNNNATC 1 cut(s) 713
BseLI CCNNNNNNNGG 1 cut(s) 660
BseMI GCAATG 1 cut(s) 1265
BseMII CTCAG 5 cut(s) 169, 424, 705, 753, 933
BseRI GAGGAG 1 cut(s) 926
BseXI GCAGC 3 cut(s) 998, 1001, 1024
Bsh1236I CGCG 1 cut(s) 1310
BshFI GGCC 1 cut(s) 70
BshNI GGYRCC 1 cut(s) 997
BsiHKAI GWGCWC 1 cut(s) 156
BsiHKCI CYCGRG 1 cut(s) 331
BsiSI CCGG 6 cut(s) 367, 393, 654, 950, 973, 983
BslI CCNNNNNNNGG 1 cut(s) 660
BsmAI GTCTC 3 cut(s) 174, 294, 920
BsmBI CGTCTC 1 cut(s) 920
BsnI GGCC 1 cut(s) 70
BsoBI CYCGRG 1 cut(s) 331
Bsp119I TTCGAA 1 cut(s) 890
Bsp1286I GDGCHC 1 cut(s) 156
Bsp143I GATC 4 cut(s) 10, 714, 1042, 1243
Bsp1720I GCTNAGC 1 cut(s) 691
BspACI CCGC 6 cut(s) 759, 1001, 1013, 1161, 1253, 1310
BspANI GGCC 1 cut(s) 70
BspCNI CTCAG 5 cut(s) 168, 423, 704, 754, 934
BspFNI CGCG 1 cut(s) 1310
BspLI GGNNCC 2 cut(s) 971, 999
BspT104I TTCGAA 1 cut(s) 890
BspT107I GGYRCC 1 cut(s) 997
BsrDI GCAATG 1 cut(s) 1265
BsrFI RCCGGY 1 cut(s) 982
BssAI RCCGGY 1 cut(s) 982
BssECI CCNNGG 4 cut(s) 674, 818, 965, 1190
BssMI GATC 4 cut(s) 10, 714, 1042, 1243
BssNI GRCGYC 1 cut(s) 998
BssT1I CCWWGG 3 cut(s) 818, 965, 1190
Bst4CI ACNGT 2 cut(s) 347, 1136
Bst6I CTCTTC 1 cut(s) 610
BstACI GRCGYC 1 cut(s) 998
BstBI TTCGAA 1 cut(s) 890
BstC8I GCNNGC 2 cut(s) 984, 1159
BstDEI CTNAG 5 cut(s) 155, 410, 691, 762, 942
BstF5I GGATG 3 cut(s) 507, 669, 853
BstFNI CGCG 1 cut(s) 1310
BstH2I RGCGCY 1 cut(s) 1001
BstHHI GCGC 2 cut(s) 1000, 1310
BstKTI GATC 4 cut(s) 13, 717, 1045, 1246
BstMAI GTCTC 3 cut(s) 174, 294, 920
BstMBI GATC 4 cut(s) 10, 714, 1042, 1243
BstMWI GCNNNNNNNGC 5 cut(s) 517, 719, 995, 1046, 1154
BstNSI RCATGY 1 cut(s) 79
BstSCI CCNGG 1 cut(s) 972
BstUI CGCG 1 cut(s) 1310
BstV1I GCAGC 3 cut(s) 998, 1001, 1024
BstV2I GAAGAC 2 cut(s) 395, 642
BstXI CCANNNNNNTGG 1 cut(s) 181
BsuI GTATCC 2 cut(s) 90, 1224
BsuRI GGCC 1 cut(s) 70
BtgZI GCGATG 1 cut(s) 282
BtsCI GGATG 3 cut(s) 507, 669, 853
BtsIMutI CAGTG 1 cut(s) 757
Cac8I GCNNGC 2 cut(s) 984, 1159
CfoI GCGC 2 cut(s) 1000, 1310
Cfr10I RCCGGY 1 cut(s) 982
Cfr13I GGNCC 1 cut(s) 1187
Csp6I GTAC 2 cut(s) 570, 1210
CviAII CATG 7 cut(s) 76, 197, 538, 611, 844, 1046, 1227
CviQI GTAC 2 cut(s) 570, 1210
DdeI CTNAG 5 cut(s) 155, 410, 691, 762, 942
DinI GGCGCC 1 cut(s) 999
DpnI GATC 4 cut(s) 12, 716, 1044, 1245
DpnII GATC 4 cut(s) 10, 714, 1042, 1243
DraI TTTAAA 1 cut(s) 363
EaeI YGGCCR 1 cut(s) 68
Eam1104I CTCTTC 1 cut(s) 610
EarI CTCTTC 1 cut(s) 610
EciI GGCGGA 1 cut(s) 1242
Ecl136II GAGCTC 1 cut(s) 154
Eco130I CCWWGG 3 cut(s) 818, 965, 1190
Eco24I GRGCYC 1 cut(s) 156
Eco47I GGWCC 1 cut(s) 1187
Eco53kI GAGCTC 1 cut(s) 154
Eco57I CTGAAG 2 cut(s) 507, 767
Eco88I CYCGRG 1 cut(s) 331
EcoICRI GAGCTC 1 cut(s) 154
EcoRI GAATTC 2 cut(s) 667, 1222
EcoT14I CCWWGG 3 cut(s) 818, 965, 1190
EcoT38I GRGCYC 1 cut(s) 156
EgeI GGCGCC 1 cut(s) 999
EheI GGCGCC 1 cut(s) 999
ErhI CCWWGG 3 cut(s) 818, 965, 1190
Esp3I CGTCTC 1 cut(s) 920
FaeI CATG 7 cut(s) 79, 200, 541, 614, 847, 1049, 1230
FatI CATG 7 cut(s) 75, 196, 537, 610, 843, 1045, 1226
FbaI TGATCA 1 cut(s) 1042
Fnu4HI GCNGC 4 cut(s) 987, 990, 1001, 1038
FokI GGATG 3 cut(s) 514, 676, 860
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 4 cut(s) 987, 990, 1001, 1038
FspBI CTAG 3 cut(s) 701, 1191, 1239
GlaI GCGC 2 cut(s) 999, 1309
GluI GCNGC 4 cut(s) 987, 990, 1001, 1038
HaeII RGCGCY 1 cut(s) 1001
HaeIII GGCC 1 cut(s) 70
HapII CCGG 6 cut(s) 367, 393, 654, 950, 973, 983
HhaI GCGC 2 cut(s) 1000, 1310
Hin1I GRCGYC 1 cut(s) 998
Hin1II CATG 7 cut(s) 79, 200, 541, 614, 847, 1049, 1230
Hin6I GCGC 2 cut(s) 998, 1308
HinP1I GCGC 2 cut(s) 998, 1308
HincII GTYRAC 1 cut(s) 1132
HindII GTYRAC 1 cut(s) 1132
HpaI GTTAAC 1 cut(s) 1132
HpaII CCGG 6 cut(s) 367, 393, 654, 950, 973, 983
HphI GGTGA 4 cut(s) 397, 920, 1175, 1324
Hpy166II GTNNAC 4 cut(s) 445, 1095, 1132, 1300
Hpy188I TCNGA 9 cut(s) 15, 58, 103, 158, 244, 675, 778, 786, 804
Hpy188III TCNNGA 1 cut(s) 412
Hpy8I GTNNAC 4 cut(s) 445, 1095, 1132, 1300
HpyAV CCTTC 1 cut(s) 531
HpyCH4III ACNGT 2 cut(s) 347, 1136
HpyF10VI GCNNNNNNNGC 5 cut(s) 517, 719, 995, 1046, 1154
HpyF3I CTNAG 5 cut(s) 155, 410, 691, 762, 942
Hsp92I GRCGYC 1 cut(s) 998
Hsp92II CATG 7 cut(s) 79, 200, 541, 614, 847, 1049, 1230
HspAI GCGC 2 cut(s) 998, 1308
KasI GGCGCC 1 cut(s) 997
KroI GCCGGC 1 cut(s) 982
KroNI GCCGGC 1 cut(s) 984
Ksp22I TGATCA 1 cut(s) 1042
KspAI GTTAAC 1 cut(s) 1132
Kzo9I GATC 4 cut(s) 10, 714, 1042, 1243
LmnI GCTCC 1 cut(s) 969
Lsp1109I GCAGC 3 cut(s) 998, 1001, 1024
MaeI CTAG 3 cut(s) 701, 1191, 1239
MaeIII GTNAC 2 cut(s) 341, 529
MalI GATC 4 cut(s) 12, 716, 1044, 1245
MboI GATC 4 cut(s) 10, 714, 1042, 1243
MfeI CAATTG 1 cut(s) 1303
MhlI GDGCHC 1 cut(s) 156
MlsI TGGCCA 1 cut(s) 70
MluCI AATT 9 cut(s) 192, 304, 505, 667, 873, 1028, 1222, 1291, 1303
MluNI TGGCCA 1 cut(s) 70
Mly113I GGCGCC 1 cut(s) 998
MlyI GAGTC 3 cut(s) 402, 706, 1118
Mox20I TGGCCA 1 cut(s) 70
MroNI GCCGGC 1 cut(s) 982
MroXI GAANNNNTTC 1 cut(s) 886
MscI TGGCCA 1 cut(s) 70
MseI TTAA 2 cut(s) 362, 1131
MslI CAYNNNNRTG 2 cut(s) 201, 538
Msp20I TGGCCA 1 cut(s) 70
MspA1I CMGCKG 1 cut(s) 761
MspI CCGG 6 cut(s) 367, 393, 654, 950, 973, 983
MspR9I CCNGG 1 cut(s) 974
MunI CAATTG 1 cut(s) 1303
MvnI CGCG 1 cut(s) 1310
MwoI GCNNNNNNNGC 5 cut(s) 517, 719, 995, 1046, 1154
NaeI GCCGGC 1 cut(s) 984
NarI GGCGCC 1 cut(s) 998
NciI CCSGG 1 cut(s) 974
NdeII GATC 4 cut(s) 10, 714, 1042, 1243
NgoMIV GCCGGC 1 cut(s) 982
NlaIII CATG 7 cut(s) 79, 200, 541, 614, 847, 1049, 1230
NlaIV GGNNCC 2 cut(s) 971, 999
NspI RCATGY 1 cut(s) 79
NspV TTCGAA 1 cut(s) 890
PaeR7I CTCGAG 1 cut(s) 331
PciI ACATGT 1 cut(s) 75
PdiI GCCGGC 1 cut(s) 984
PdmI GAANNNNTTC 1 cut(s) 886
PkrI GCNGC 4 cut(s) 988, 991, 1002, 1039
PleI GAGTC 3 cut(s) 402, 705, 1118
PluTI GGCGCC 1 cut(s) 1001
PpsI GAGTC 3 cut(s) 402, 705, 1118
PscI ACATGT 1 cut(s) 75
Psp124BI GAGCTC 1 cut(s) 156
PspN4I GGNNCC 2 cut(s) 971, 999
PspPI GGNCC 1 cut(s) 1187
PspXI VCTCGAGB 1 cut(s) 331
RsaI GTAC 2 cut(s) 571, 1211
RsaNI GTAC 2 cut(s) 570, 1210
RseI CAYNNNNRTG 2 cut(s) 201, 538
SacI GAGCTC 1 cut(s) 156
SaqAI TTAA 2 cut(s) 362, 1131
SatI GCNGC 4 cut(s) 987, 990, 1001, 1038
Sau3AI GATC 4 cut(s) 10, 714, 1042, 1243
Sau96I GGNCC 1 cut(s) 1187
SchI GAGTC 3 cut(s) 402, 706, 1118
ScrFI CCNGG 1 cut(s) 974
SduI GDGCHC 1 cut(s) 156
SfoI GGCGCC 1 cut(s) 999
Sfr274I CTCGAG 1 cut(s) 331
SfuI TTCGAA 1 cut(s) 890
SinI GGWCC 1 cut(s) 1187
SlaI CTCGAG 1 cut(s) 331
SmiMI CAYNNNNRTG 2 cut(s) 201, 538
SmlI CTYRAG 2 cut(s) 40, 331
SmoI CTYRAG 2 cut(s) 40, 331
Sse9I AATT 9 cut(s) 192, 304, 505, 667, 873, 1028, 1222, 1291, 1303
SsiI CCGC 6 cut(s) 759, 1001, 1013, 1161, 1253, 1310
SspDI GGCGCC 1 cut(s) 997
SspMI CTAG 3 cut(s) 701, 1191, 1239
SstI GAGCTC 1 cut(s) 156
StyD4I CCNGG 1 cut(s) 972
StyI CCWWGG 3 cut(s) 818, 965, 1190
TaaI ACNGT 2 cut(s) 347, 1136
TaqI TCGA 5 cut(s) 332, 350, 890, 1246, 1289
TaqII GACCGA 1 cut(s) 366
TasI AATT 9 cut(s) 192, 304, 505, 667, 873, 1028, 1222, 1291, 1303
TauI GCSGC 1 cut(s) 1003
Tru1I TTAA 2 cut(s) 362, 1131
Tru9I TTAA 2 cut(s) 362, 1131
TscAI CASTG 1 cut(s) 757
TseI GCWGC 3 cut(s) 986, 989, 1037
TspGWI ACGGA 1 cut(s) 587
TspRI CASTG 1 cut(s) 757
VpaK11BI GGWCC 1 cut(s) 1187
XapI RAATTY 4 cut(s) 505, 667, 1222, 1291
XceI RCATGY 1 cut(s) 79
XhoI CTCGAG 1 cut(s) 331
XmaJI CCTAGG 1 cut(s) 1190
XmnI GAANNNNTTC 1 cut(s) 886
XspI CTAG 3 cut(s) 701, 1191, 1239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.