AT1G64660

methionine gamma-lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012000
Physical Location & Seq
Forward (+)
24028830 .. 24030780
1951 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G64660.1

Sequence Viewer

Length: 1326 bp
ATGGCTCATTTCCTCGAGACACAGGAGCCCCTCGTATTCTCCGGGAAGAAACGAAACGATCGCGATGACGAAGACGGTGACGCCTTGGTTGCGAAGAAATCAGCACTGGCGGTGTGTGATGCCGACCCTGCAGCGGCCATAGCGAATATCCGACATGAGTTTGGGGAGCACGGGGGAGTAAACATGTCCATCGAAGCCTCCGCCACCTTCACCGTCATGGAACCGGATACCATGAGGCGGATGTTCACCGGAGAACTGGGACCGGACAACGACTTCTACGTATACAGCCGACACTTCAACCCCACCGTGCTCAACCTCAGCCGCCAGATGGCTGCTCTCGAAGGCACCCAAGCTGCCTACTGTACCTCTAGCGGTATGTCGGCGATATCGTCAGTGATGCTGCAACTGTGCAGCAGCGGAGGACACGTGGTTGCGGCAAGCACGCTATACGGAGGAACACACGCTTTGCTCTCTCATTTCTTGCCACGGACATGTAACATAACCACCTCCTTCGTTGACATAACGGACCATGGCGCCGTGGCAAACGCGATCGTTGAGGGTAGGACACAGGTTCTCTACTTTGAGTCGGTGGCAAACCCGACGCTGACTGTGGCTGACATACCTGAGCTGAGCCGTATGGCACACGAGAAGGGCGTGACGGTGGTGGTGGACAACACATTCGCCCCCATGGTGCTGTCTCCGGCCAAGCTTGGAGCAGATGTGGTGGTTCACAGTATCTCCAAGTTCATCAGTGGTGGGGCTGACATCATCGCAGGGGCCGTGTGTGGGAGTGAGAATTTGGTGAAAGAGATGATGGATCTTCGTGGCGGATCTCTGATGCTTCTAGGTCCCACCATGAACGCCAAGGTGGCTTTCGAGCTCTCCGAGCGAATCCCTCACTTGGGCCTACGCATGAGAGAGCACAGCCACAGAGCCCAAGTGTATGCGGAGAGAATGAGGGATTTGGGCATGAAAGTCATATATCCAGGGCTCGAGACCCACCCGCAGCACAAGCTATTCAAAGGGATGGTGAATAGAGACTATGGATACGGAGGATTGCTGTCGATAGACATGGAGACAGAGGAGAAAGCCAACAAGCTCATGGCATATCTCCAGAACGCCACTCAGTTTGGCTTCATGGCCGTCAGTTTGGGTTACTACGAGACACTCATGTCTTGCTCCGGGAGCAGCACTAGCAGCGAGCTTGACCCCTCACAGAAGGAAGCTGCAGGCATCTCTCCTGGTCTTGTACGAATGTCGGTGGGGTATGTGGGTACATTGGAGCAGAAGTGGACACAATTCGAGAAAGCATTCCTCAGAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000096 GO:0000097 GO:0000098 GO:0001101 GO:0003674 GO:0003824 GO:0003962 GO:0004123 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006520 GO:0006534 GO:0006555 GO:0006790 GO:0006807 GO:0006950 GO:0007154 GO:0008144 GO:0008150 GO:0008152 GO:0008652 GO:0009056 GO:0009058 GO:0009063 GO:0009066 GO:0009067 GO:0009068 GO:0009069 GO:0009070 GO:0009086 GO:0009087 GO:0009092 GO:0009267 GO:0009414 GO:0009415 GO:0009605 GO:0009628 GO:0009970 GO:0009987 GO:0009991 GO:0010035 GO:0016043 GO:0016053 GO:0016054 GO:0016740 GO:0016765 GO:0016829 GO:0016846 GO:0017144 GO:0018826 GO:0019343 GO:0019344 GO:0019346 GO:0019458 GO:0019752 GO:0019842 GO:0022607 GO:0030170 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0036094 GO:0042221 GO:0042594 GO:0042631 GO:0043167 GO:0043168 GO:0043436 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044272 GO:0044273 GO:0044281 GO:0044282 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046395 GO:0048037 GO:0050662 GO:0050667 GO:0050896 GO:0051259 GO:0051260 GO:0051262 GO:0051289 GO:0051716 GO:0065003 GO:0070279 GO:0070887 GO:0071214 GO:0071229 GO:0071265 GO:0071266 GO:0071462 GO:0071496 GO:0071704 GO:0071840 GO:0097159 GO:0104004 GO:1901363 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901605 GO:1901606 GO:1901607 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
ko00270 ko00450 map00270 map00450
Pfam Domains
Protein Families
Cys_Met_Meta_PP

Protein Analysis

441

Amino Acids

47.82

Weight (kDa)

5.68

Isoelectric Point (pI)

31.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cys_Met_Meta_PP PF01053 55 - 422 4.4e-85 Cys/Met metabolism PLP-dependent enzyme
Aminotran_1_2 PF00155 113 - 324 1.8e-08 Aminotransferase class I and II
Aminotran_5 PF00266 122 - 290 2.2e-08 Aminotransferase class-V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012000)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64660
fragaria_vesca FvH4_5g10890 FvH4_5g10890 FvH4_5g10890
malus_domestica MD06G1118700.v1.1 MD14G1126800.v1.1
prunus_persica Prupe.5G129800_v2.0.a1
pyrus_communis pycom06g11020 pycom14g11140
rosa_chinensis RchiOBHm_Chr7g0186971
rosa_laevigata RLG00000004805
rosa_multiflora Rmu_sc0007656.1_g000001
rosa_roxburghii Rroxscaffold_3G00267720
rosa_rugosa Rorug06G0478300
rosa_samantha Rh7AG083900 Rh7BG079000 Rh7CG084900 Rh7DG085800
rosa_wichuraiana Rw7G007380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1171
AccB1I GGYRCC 2 cut(s) 344, 533
AccI GTMKAC 1 cut(s) 282
AccII CGCG 2 cut(s) 63, 548
AclWI GGATC 2 cut(s) 825, 838
AcoI YGGCCR 3 cut(s) 135, 702, 1140
AcsI RAATTY 1 cut(s) 796
AcvI CACGTG 1 cut(s) 427
AcyI GRCGYC 2 cut(s) 81, 534
AfaI GTAC 3 cut(s) 364, 1251, 1276
AfiI CCNNNNNNNGG 6 cut(s) 133, 237, 328, 786, 901, 902
AflIII ACRYGT 3 cut(s) 183, 424, 491
AgsI TTSAA 2 cut(s) 298, 1021
AjnI CCWGG 2 cut(s) 985, 1240
AjuI GAANNNNNNNTTGG 2 cut(s) 857, 889
AluBI AGCT 8 cut(s) 353, 628, 709, 880, 1015, 1099, 1204, 1226
AluI AGCT 8 cut(s) 353, 628, 709, 880, 1015, 1099, 1204, 1226
Alw21I GWGCWC 4 cut(s) 171, 312, 882, 924
Alw26I GTCTC 6 cut(s) 11, 702, 989, 1032, 1070, 1157
AlwI GGATC 2 cut(s) 825, 838
Ama87I CYCGRG 2 cut(s) 14, 992
AoxI GGCC 5 cut(s) 135, 702, 777, 904, 1140
ApoI RAATTY 1 cut(s) 796
ArsI GACNNNNNNTTYG 2 cut(s) 662, 694
Asp700I GAANNNNTTC 1 cut(s) 1310
AspLEI GCGC 1 cut(s) 536
AspS9I GGNCC 5 cut(s) 260, 526, 777, 848, 904
AsuC2I CCSGG 2 cut(s) 43, 1183
AsuHPI GGTGA 5 cut(s) 89, 202, 238, 814, 1042
AvaI CYCGRG 2 cut(s) 14, 992
AvaII GGWCC 3 cut(s) 260, 526, 848
BanI GGYRCC 2 cut(s) 344, 533
BanII GRGCYC 4 cut(s) 30, 882, 937, 993
BauI CACGAG 1 cut(s) 644
BbrPI CACGTG 1 cut(s) 427
BbsI GAAGAC 1 cut(s) 78
Bbv12I GWGCWC 4 cut(s) 171, 312, 882, 924
BbvCI CCTCAGC 1 cut(s) 317
BccI CCATC 4 cut(s) 197, 322, 808, 1021
BceAI ACGGC 4 cut(s) 521, 618, 764, 1127
BciT130I CCWGG 2 cut(s) 987, 1242
BciVI GTATCC 2 cut(s) 220, 1040
BcnI CCSGG 2 cut(s) 43, 1183
BcoDI GTCTC 6 cut(s) 11, 702, 989, 1032, 1070, 1157
BfaI CTAG 3 cut(s) 369, 845, 1194
BfmI CTRYAG 2 cut(s) 129, 1227
BfoI RGCGCY 1 cut(s) 537
BfuI GTATCC 2 cut(s) 220, 1040
BglI GCCNNNNNGGC 1 cut(s) 869
BlpI GCTNAGC 1 cut(s) 629
Bme1390I CCNGG 4 cut(s) 43, 987, 1183, 1242
Bme18I GGWCC 3 cut(s) 260, 526, 848
BmeT110I CYCGRG 2 cut(s) 14, 992
BmgT120I GGNCC 5 cut(s) 260, 526, 777, 848, 904
BmiI GGNNCC 7 cut(s) 27, 222, 261, 346, 535, 778, 850
BmrFI CCNGG 4 cut(s) 43, 987, 1183, 1242
BmrI ACTGGG 1 cut(s) 266
BmsI GCATC 4 cut(s) 109, 387, 828, 1242
BmuI ACTGGG 1 cut(s) 266
BpiI GAAGAC 1 cut(s) 78
BpmI CTGGAG 1 cut(s) 1097
Bpu10I CCTNAGC 2 cut(s) 317, 624
Bpu1102I GCTNAGC 1 cut(s) 629
BpuMI CCSGG 2 cut(s) 43, 1183
BsaAI YACGTR 2 cut(s) 280, 427
BsaHI GRCGYC 2 cut(s) 81, 534
BsaI GGTCTC 1 cut(s) 989
BsaJI CCNNGG 7 cut(s) 84, 485, 529, 537, 687, 864, 986
BsaWI WCCGGW 3 cut(s) 223, 248, 262
BsaXI ACNNNNNCTCC 8 cut(s) 411, 441, 705, 722, 735, 752, 1274, 1304
Bsc4I CCNNNNNNNGG 6 cut(s) 133, 237, 328, 786, 901, 902
Bse1I ACTGG 2 cut(s) 111, 261
BseBI CCWGG 2 cut(s) 987, 1242
BseDI CCNNGG 7 cut(s) 84, 485, 529, 537, 687, 864, 986
BseGI GGATG 2 cut(s) 246, 1032
BseLI CCNNNNNNNGG 6 cut(s) 133, 237, 328, 786, 901, 902
BseMII CTCAG 4 cut(s) 331, 615, 620, 1139
BseNI ACTGG 2 cut(s) 111, 261
BseRI GAGGAG 1 cut(s) 1097
BsgI GTGCAG 1 cut(s) 430
Bsh1236I CGCG 2 cut(s) 63, 548
Bsh1285I CGRYCG 2 cut(s) 61, 552
BshFI GGCC 5 cut(s) 137, 704, 779, 906, 1142
BshNI GGYRCC 2 cut(s) 344, 533
BsiEI CGRYCG 2 cut(s) 61, 552
BsiHKAI GWGCWC 4 cut(s) 171, 312, 882, 924
BsiHKCI CYCGRG 2 cut(s) 14, 992
BsiSI CCGG 6 cut(s) 42, 224, 249, 263, 701, 1182
BslFI GGGAC 2 cut(s) 273, 834
BslI CCNNNNNNNGG 6 cut(s) 133, 237, 328, 786, 901, 902
BsmAI GTCTC 6 cut(s) 11, 702, 989, 1032, 1070, 1157
BsmFI GGGAC 2 cut(s) 273, 834
BsmI GAATGC 1 cut(s) 1310
BsnI GGCC 5 cut(s) 137, 704, 779, 906, 1142
Bso31I GGTCTC 1 cut(s) 989
BsoBI CYCGRG 2 cut(s) 14, 992
Bsp1286I GDGCHC 7 cut(s) 30, 171, 312, 882, 924, 937, 993
Bsp143I GATC 4 cut(s) 58, 549, 817, 830
Bsp1720I GCTNAGC 1 cut(s) 629
Bsp19I CCATGG 2 cut(s) 529, 687
Bsp68I TCGCGA 1 cut(s) 63
BspANI GGCC 5 cut(s) 137, 704, 779, 906, 1142
BspCNI CTCAG 4 cut(s) 330, 616, 621, 1138
BspFNI CGCG 2 cut(s) 63, 548
BspLI GGNNCC 7 cut(s) 27, 222, 261, 346, 535, 778, 850
BspMAI CTGCAG 2 cut(s) 133, 1231
BspPI GGATC 2 cut(s) 825, 838
BspT107I GGYRCC 2 cut(s) 344, 533
BspTNI GGTCTC 1 cut(s) 989
BsrI ACTGG 2 cut(s) 111, 261
BssECI CCNNGG 7 cut(s) 84, 485, 529, 537, 687, 864, 986
BssMI GATC 4 cut(s) 58, 549, 817, 830
BssNAI GTATAC 1 cut(s) 283
BssNI GRCGYC 2 cut(s) 81, 534
BssSI CACGAG 1 cut(s) 644
BssT1I CCWWGG 4 cut(s) 84, 529, 687, 864
Bst1107I GTATAC 1 cut(s) 283
Bst2BI CACGAG 1 cut(s) 644
Bst2UI CCWGG 2 cut(s) 987, 1242
Bst4CI ACNGT 8 cut(s) 77, 214, 307, 362, 408, 610, 661, 734
BstACI GRCGYC 2 cut(s) 81, 534
BstBAI YACGTR 2 cut(s) 280, 427
BstC8I GCNNGC 4 cut(s) 439, 443, 1202, 1231
BstDEI CTNAG 5 cut(s) 317, 624, 629, 1125, 1316
BstDSI CCRYGG 4 cut(s) 485, 529, 537, 687
BstF5I GGATG 2 cut(s) 246, 1032
BstFNI CGCG 2 cut(s) 63, 548
BstH2I RGCGCY 1 cut(s) 537
BstHHI GCGC 1 cut(s) 536
BstKTI GATC 4 cut(s) 61, 552, 820, 833
BstMAI GTCTC 6 cut(s) 11, 702, 989, 1032, 1070, 1157
BstMBI GATC 4 cut(s) 58, 549, 817, 830
BstMCI CGRYCG 2 cut(s) 61, 552
BstMWI GCNNNNNNNGC 9 cut(s) 89, 128, 140, 869, 886, 1012, 1185, 1194, 1197
BstNI CCWGG 2 cut(s) 987, 1242
BstNSI RCATGY 2 cut(s) 187, 495
BstSCI CCNGG 4 cut(s) 41, 985, 1181, 1240
BstSFI CTRYAG 2 cut(s) 129, 1227
BstSNI TACGTA 1 cut(s) 280
BstUI CGCG 2 cut(s) 63, 548
BstV2I GAAGAC 1 cut(s) 78
BstX2I RGATCY 2 cut(s) 817, 830
BstYI RGATCY 2 cut(s) 817, 830
BstZ17I GTATAC 1 cut(s) 283
BsuI GTATCC 2 cut(s) 220, 1040
BsuRI GGCC 5 cut(s) 137, 704, 779, 906, 1142
BtgI CCRYGG 4 cut(s) 485, 529, 537, 687
BtgZI GCGATG 2 cut(s) 78, 754
BtsCI GGATG 2 cut(s) 246, 1032
BtsIMutI CAGTG 3 cut(s) 104, 399, 757
BtuMI TCGCGA 1 cut(s) 63
Cac8I GCNNGC 4 cut(s) 439, 443, 1202, 1231
CfoI GCGC 1 cut(s) 536
Cfr13I GGNCC 5 cut(s) 260, 526, 777, 848, 904
CseI GACGC 2 cut(s) 89, 610
Csp6I GTAC 3 cut(s) 363, 1250, 1275
CspCI CAANNNNNGTGG 2 cut(s) 1111, 1146
CviQI GTAC 3 cut(s) 363, 1250, 1275
DdeI CTNAG 5 cut(s) 317, 624, 629, 1125, 1316
DinI GGCGCC 1 cut(s) 535
DpnI GATC 4 cut(s) 60, 551, 819, 832
DpnII GATC 4 cut(s) 58, 549, 817, 830
DrdI GACNNNNNNGTC 1 cut(s) 1171
DseDI GACNNNNNNGTC 1 cut(s) 1171
EaeI YGGCCR 3 cut(s) 135, 702, 1140
EciI GGCGGA 3 cut(s) 190, 253, 843
Ecl136II GAGCTC 1 cut(s) 880
Eco105I TACGTA 1 cut(s) 280
Eco130I CCWWGG 4 cut(s) 84, 529, 687, 864
Eco24I GRGCYC 4 cut(s) 30, 882, 937, 993
Eco31I GGTCTC 1 cut(s) 989
Eco32I GATATC 1 cut(s) 387
Eco47I GGWCC 3 cut(s) 260, 526, 848
Eco53kI GAGCTC 1 cut(s) 880
Eco72I CACGTG 1 cut(s) 427
Eco88I CYCGRG 2 cut(s) 14, 992
EcoICRI GAGCTC 1 cut(s) 880
EcoO109I RGGNCCY 1 cut(s) 848
EcoRII CCWGG 2 cut(s) 985, 1240
EcoRV GATATC 1 cut(s) 387
EcoT14I CCWWGG 4 cut(s) 84, 529, 687, 864
EcoT38I GRGCYC 4 cut(s) 30, 882, 937, 993
EgeI GGCGCC 1 cut(s) 535
EheI GGCGCC 1 cut(s) 535
ErhI CCWWGG 4 cut(s) 84, 529, 687, 864
FaqI GGGAC 2 cut(s) 273, 834
FauI CCCGC 1 cut(s) 1011
FblI GTMKAC 1 cut(s) 282
FokI GGATG 2 cut(s) 253, 1039
FriOI GRGCYC 4 cut(s) 30, 882, 937, 993
FspBI CTAG 3 cut(s) 369, 845, 1194
GlaI GCGC 1 cut(s) 535
GsuI CTGGAG 1 cut(s) 1097
HaeII RGCGCY 1 cut(s) 537
HaeIII GGCC 5 cut(s) 137, 704, 779, 906, 1142
HapII CCGG 6 cut(s) 42, 224, 249, 263, 701, 1182
HgaI GACGC 2 cut(s) 89, 610
HhaI GCGC 1 cut(s) 536
Hin1I GRCGYC 2 cut(s) 81, 534
Hin6I GCGC 1 cut(s) 534
HinP1I GCGC 1 cut(s) 534
HincII GTYRAC 1 cut(s) 517
HindII GTYRAC 1 cut(s) 517
HindIII AAGCTT 1 cut(s) 707
HinfI GANTC 2 cut(s) 584, 891
HpaII CCGG 6 cut(s) 42, 224, 249, 263, 701, 1182
HphI GGTGA 5 cut(s) 89, 202, 238, 814, 1042
Hpy166II GTNNAC 7 cut(s) 181, 246, 283, 517, 670, 730, 1293
Hpy188I TCNGA 4 cut(s) 152, 837, 886, 1319
Hpy188III TCNNGA 6 cut(s) 16, 62, 338, 994, 1114, 1303
Hpy8I GTNNAC 7 cut(s) 181, 246, 283, 517, 670, 730, 1293
Hpy99I CGWCG 1 cut(s) 604
HpyAV CCTTC 5 cut(s) 217, 335, 520, 643, 1213
HpyCH4III ACNGT 8 cut(s) 77, 214, 307, 362, 408, 610, 661, 734
HpyCH4IV ACGT 2 cut(s) 279, 426
HpyCH4V TGCA 4 cut(s) 131, 403, 411, 1229
HpyF10VI GCNNNNNNNGC 9 cut(s) 89, 128, 140, 869, 886, 1012, 1185, 1194, 1197
HpyF3I CTNAG 5 cut(s) 317, 624, 629, 1125, 1316
HpySE526I ACGT 2 cut(s) 279, 426
Hsp92I GRCGYC 2 cut(s) 81, 534
HspAI GCGC 1 cut(s) 534
KasI GGCGCC 1 cut(s) 533
Kzo9I GATC 4 cut(s) 58, 549, 817, 830
LmnI GCTCC 6 cut(s) 25, 166, 713, 1184, 1185, 1282
LweI GCATC 4 cut(s) 109, 387, 828, 1242
MaeI CTAG 3 cut(s) 369, 845, 1194
MaeII ACGT 2 cut(s) 279, 426
MaeIII GTNAC 4 cut(s) 77, 494, 655, 1154
MalI GATC 4 cut(s) 60, 551, 819, 832
MboI GATC 4 cut(s) 58, 549, 817, 830
MboII GAAGA 4 cut(s) 58, 83, 106, 812
MflI RGATCY 2 cut(s) 817, 830
MhlI GDGCHC 7 cut(s) 30, 171, 312, 882, 924, 937, 993
MluCI AATT 2 cut(s) 796, 1298
Mly113I GGCGCC 1 cut(s) 534
MlyI GAGTC 1 cut(s) 593
MmeI TCCRAC 1 cut(s) 175
MroXI GAANNNNTTC 1 cut(s) 1310
MslI CAYNNNNRTG 2 cut(s) 215, 490
MspA1I CMGCKG 2 cut(s) 134, 417
MspI CCGG 6 cut(s) 42, 224, 249, 263, 701, 1182
MspR9I CCNGG 4 cut(s) 43, 987, 1183, 1242
Mva1269I GAATGC 1 cut(s) 1310
MvaI CCWGG 2 cut(s) 987, 1242
MvnI CGCG 2 cut(s) 63, 548
MwoI GCNNNNNNNGC 9 cut(s) 89, 128, 140, 869, 886, 1012, 1185, 1194, 1197
NarI GGCGCC 1 cut(s) 534
NciI CCSGG 2 cut(s) 43, 1183
NcoI CCATGG 2 cut(s) 529, 687
NdeII GATC 4 cut(s) 58, 549, 817, 830
NlaIV GGNNCC 7 cut(s) 27, 222, 261, 346, 535, 778, 850
NmuCI GTSAC 2 cut(s) 77, 655
NruI TCGCGA 1 cut(s) 63
NspI RCATGY 2 cut(s) 187, 495
PaeR7I CTCGAG 2 cut(s) 14, 992
PciI ACATGT 2 cut(s) 183, 491
PcsI WCGNNNNNNNCGW 5 cut(s) 276, 386, 651, 777, 882
PctI GAATGC 1 cut(s) 1310
PdmI GAANNNNTTC 1 cut(s) 1310
PfeI GAWTC 1 cut(s) 891
PfoI TCCNGGA 2 cut(s) 41, 1181
Ple19I CGATCG 2 cut(s) 61, 552
PleI GAGTC 1 cut(s) 592
PluTI GGCGCC 1 cut(s) 537
PmaCI CACGTG 1 cut(s) 427
PmlI CACGTG 1 cut(s) 427
PpsI GAGTC 1 cut(s) 592
Ppu21I YACGTR 2 cut(s) 280, 427
PpuMI RGGWCCY 1 cut(s) 848
PscI ACATGT 2 cut(s) 183, 491
Psp124BI GAGCTC 1 cut(s) 882
Psp5II RGGWCCY 1 cut(s) 848
Psp6I CCWGG 2 cut(s) 985, 1240
PspCI CACGTG 1 cut(s) 427
PspGI CCWGG 2 cut(s) 985, 1240
PspN4I GGNNCC 7 cut(s) 27, 222, 261, 346, 535, 778, 850
PspPI GGNCC 5 cut(s) 260, 526, 777, 848, 904
PspPPI RGGWCCY 1 cut(s) 848
PstI CTGCAG 2 cut(s) 133, 1231
PsuI RGATCY 2 cut(s) 817, 830
PvuI CGATCG 2 cut(s) 61, 552
RruI TCGCGA 1 cut(s) 63
RsaI GTAC 3 cut(s) 364, 1251, 1276
RsaNI GTAC 3 cut(s) 363, 1250, 1275
RseI CAYNNNNRTG 2 cut(s) 215, 490
SacI GAGCTC 1 cut(s) 882
Sau3AI GATC 4 cut(s) 58, 549, 817, 830
Sau96I GGNCC 5 cut(s) 260, 526, 777, 848, 904
SchI GAGTC 1 cut(s) 593
ScrFI CCNGG 4 cut(s) 43, 987, 1183, 1242
SduI GDGCHC 7 cut(s) 30, 171, 312, 882, 924, 937, 993
SfaNI GCATC 4 cut(s) 109, 387, 828, 1242
SfcI CTRYAG 2 cut(s) 129, 1227
SfoI GGCGCC 1 cut(s) 535
Sfr274I CTCGAG 2 cut(s) 14, 992
SinI GGWCC 3 cut(s) 260, 526, 848
SlaI CTCGAG 2 cut(s) 14, 992
SmiMI CAYNNNNRTG 2 cut(s) 215, 490
SmlI CTYRAG 2 cut(s) 14, 992
SmoI CTYRAG 2 cut(s) 14, 992
SnaBI TACGTA 1 cut(s) 280
Sse9I AATT 2 cut(s) 796, 1298
SspDI GGCGCC 1 cut(s) 533
SspMI CTAG 3 cut(s) 369, 845, 1194
SstI GAGCTC 1 cut(s) 882
StyD4I CCNGG 4 cut(s) 41, 985, 1181, 1240
StyI CCWWGG 4 cut(s) 84, 529, 687, 864
TaaI ACNGT 8 cut(s) 77, 214, 307, 362, 408, 610, 661, 734
TaiI ACGT 2 cut(s) 282, 429
TaqI TCGA 7 cut(s) 15, 192, 339, 876, 993, 1064, 1302
TasI AATT 2 cut(s) 796, 1298
TauI GCSGC 3 cut(s) 137, 324, 437
TfiI GAWTC 1 cut(s) 891
TscAI CASTG 3 cut(s) 111, 399, 757
TseFI GTSAC 2 cut(s) 77, 655
Tsp45I GTSAC 2 cut(s) 77, 655
TspDTI ATGAA 4 cut(s) 736, 872, 986, 1126
TspGWI ACGGA 4 cut(s) 465, 502, 539, 1065
TspRI CASTG 3 cut(s) 111, 399, 757
VpaK11BI GGWCC 3 cut(s) 260, 526, 848
XapI RAATTY 1 cut(s) 796
XceI RCATGY 2 cut(s) 187, 495
XcmI CCANNNNNNNNNTGG 1 cut(s) 1099
XhoI CTCGAG 2 cut(s) 14, 992
XmiI GTMKAC 1 cut(s) 282
XmnI GAANNNNTTC 1 cut(s) 1310
XspI CTAG 3 cut(s) 369, 845, 1194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.