MD14G1126800.v1.1

Methionine gamma-lyase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012000
Physical Location & Seq
Forward (+)
20152604 .. 20155833
3230 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1126800.v1.1.491

Sequence Viewer

Length: 1374 bp
ATGGCTGACACCAAATCCCAGGGATTAGTTTTCCCCACCATCAACAGCAAGAAGAGAGCAGAGCACGACGACATAGACAACGAAGACTATGTTGCCGCCAAAAAGTCCATGTTGTTGTCCTCGGCTGCAGCTGCAGCCTGGGAAGACCCAGCCGCGGCATTAGCCAGTGCTCGCCATGAGTTTGGTGAGCACGGTGGTGTCAACATGTCGATTGAGGCCTCCGCCACGTTCACCGTGATGGAGCCCGAGACTCTTCGCAAAATGTTCTCCGGCGAGCTGGGTGCCGACCGCGACTTCTTCATCTACAGCCGCCACTTTAACCCTACCGTGCTCAATCTCAGTCGCCAGATGGCAGCCCTCGAGGGCACAGAGGCCGCCTACTGCACGTCCTCTGGGATGTCAGCCATATCCTCCGTCCTGCTCCAGCTCGTCAGCAGCGGGGAGCACATCGTAGCCTCAAGAACCGTCTACGGAGGGACCCACGCCCTCATGTCCCACTTCTTCCCCAGGGCATGCAACATAACGACGACGTTTGTGGACATCAACGACATGGACATGGTGAGGAATGCTATTGAGGTCGGGAAGACCAAGGTACTCTATTTCGAAGGCATGTCGAACCCAACTCTCACCGTCGCCAACATACCGGAGCTCAGCCGTATCGGCCACGAGAAGGGGGTCACGGTTGTGGTGGACAACACGTTTTCTCCCATGGTTCTCTCTCCGGTGAGGCTTGGTGCTGACGTTGTTGTTCACAGCATTTCCAAGTTTATTAGCGGCGGTGCTGATATTATTGCAGGTGTTGTGTGCGGACCTGCAAGCCTGGTGAACTCAATGATGGATCTACACCAAGGCTCCCTAATGCTCTTGGGTCCCACAATGAATGCCAAGGTGGCATTTGAGCTCTCTGAGAGGATTCCCCACCTAGGGTTGAGAATGAAGGAGCACTGCCATAGGGCAATGGTTTATGCCCAAAGGATGAAGAAGATGGGCTTAAAAGTAATTTACCCGGGGCTCGACGACCACCCGCAGCACGAGCTTCTCAAGGCCATAAGAAACAAAGATTACGGGTACGGAGGGCTTCTGTGTTTGGACATGGGGACTGAGGAAAGAGCCAATAGGCTCATGAATCTCTTGCAGAACTGCACACAGTTTGGGTTCATGGCTGTGAGCCTAGGGTACTATGAAACTCTAATGTCCTGCTCTGGAAGCAGCACAAGCAGTGAGATGAATGATGAGGAGAAGGCTCTGGCTGGGATCTCACCAGGGCTCGTGAGGATGTCGATCGGGTACATTGGGACGTTGGAGCAGAGGTGGAGCCAATTTGAGAAGGCAATTTCTAGAATGCAGGAGTCTGGTTTGTTGAATAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00450 map00270 map00450
Pfam Domains
Protein Families
Cys_Met_Meta_PP

Protein Analysis

458

Amino Acids

49.85

Weight (kDa)

6.12

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cys_Met_Meta_PP PF01053 62 - 429 9.6e-83 Cys/Met metabolism PLP-dependent enzyme
Aminotran_1_2 PF00155 116 - 333 9.8e-10 Aminotransferase class I and II
Aminotran_5 PF00266 132 - 297 3.7e-06 Aminotransferase class-V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012000)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64660
fragaria_vesca FvH4_5g10890 FvH4_5g10890 FvH4_5g10890
malus_domestica MD06G1118700.v1.1 MD14G1126800.v1.1
prunus_persica Prupe.5G129800_v2.0.a1
pyrus_communis pycom06g11020 pycom14g11140
rosa_chinensis RchiOBHm_Chr7g0186971
rosa_laevigata RLG00000004805
rosa_multiflora Rmu_sc0007656.1_g000001
rosa_roxburghii Rroxscaffold_3G00267720
rosa_rugosa Rorug06G0478300
rosa_samantha Rh7AG083900 Rh7BG079000 Rh7CG084900 Rh7DG085800
rosa_wichuraiana Rw7G007380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 785
AbsI CCTCGAGG 1 cut(s) 359
Acc36I ACCTGC 2 cut(s) 785, 820
AccB1I GGYRCC 1 cut(s) 281
AccI GTMKAC 1 cut(s) 468
AccII CGCG 2 cut(s) 155, 291
AclWI GGATC 2 cut(s) 846, 1262
AcoI YGGCCR 1 cut(s) 661
AfaI GTAC 4 cut(s) 594, 1070, 1178, 1289
AfiI CCNNNNNNNGG 4 cut(s) 154, 670, 923, 924
AflIII ACRYGT 2 cut(s) 204, 696
AgsI TTSAA 1 cut(s) 1363
AjiI CACGTC 1 cut(s) 387
AjnI CCWGG 5 cut(s) 18, 137, 506, 819, 1261
AleI CACNNNNGTG 2 cut(s) 195, 683
AluBI AGCT 6 cut(s) 131, 277, 427, 649, 901, 1036
AluI AGCT 6 cut(s) 131, 277, 427, 649, 901, 1036
Alw21I GWGCWC 8 cut(s) 66, 172, 192, 333, 447, 651, 903, 945
Alw26I GTCTC 1 cut(s) 242
AlwI GGATC 2 cut(s) 846, 1262
Ama87I CYCGRG 3 cut(s) 245, 359, 1006
AoxI GGCC 4 cut(s) 216, 372, 661, 1044
ApeKI GCWGC 8 cut(s) 125, 128, 131, 134, 353, 435, 1027, 1209
AspA2I CCTAGG 2 cut(s) 922, 1171
AspS9I GGNCC 3 cut(s) 477, 809, 869
AsuC2I CCSGG 2 cut(s) 1007, 1008
AsuHPI GGTGA 7 cut(s) 197, 223, 571, 619, 736, 835, 1251
AsuII TTCGAA 1 cut(s) 603
AvaI CYCGRG 3 cut(s) 245, 359, 1006
AvaII GGWCC 3 cut(s) 477, 809, 869
AvrII CCTAGG 2 cut(s) 922, 1171
BaeGI GKGCMC 1 cut(s) 368
BanI GGYRCC 1 cut(s) 281
BanII GRGCYC 5 cut(s) 246, 651, 903, 1014, 1269
BauI CACGAG 3 cut(s) 665, 1031, 1268
BbsI GAAGAC 3 cut(s) 90, 150, 590
Bbv12I GWGCWC 8 cut(s) 66, 172, 192, 333, 447, 651, 903, 945
BbvI GCAGC 8 cut(s) 112, 118, 140, 146, 365, 447, 1039, 1221
BccI CCATC 5 cut(s) 47, 232, 343, 829, 979
BceAI ACGGC 1 cut(s) 639
BcgI CGANNNNNNTGC 2 cut(s) 263, 297
BciT130I CCWGG 5 cut(s) 20, 139, 508, 821, 1263
BcnI CCSGG 2 cut(s) 1007, 1008
BcoDI GTCTC 1 cut(s) 242
BfaI CTAG 3 cut(s) 923, 1172, 1338
BfmI CTRYAG 3 cut(s) 126, 132, 304
BfuAI ACCTGC 2 cut(s) 785, 820
BglI GCCNNNNNGGC 2 cut(s) 660, 890
BlnI CCTAGG 2 cut(s) 922, 1171
BlpI GCTNAGC 1 cut(s) 650
Bme1390I CCNGG 7 cut(s) 20, 139, 508, 821, 1007, 1008, 1263
Bme18I GGWCC 3 cut(s) 477, 809, 869
BmeT110I CYCGRG 3 cut(s) 245, 359, 1006
BmgBI CACGTC 1 cut(s) 387
BmgT120I GGNCC 3 cut(s) 477, 809, 869
BmiI GGNNCC 8 cut(s) 243, 283, 478, 479, 853, 870, 871, 1316
BmrFI CCNGG 7 cut(s) 20, 139, 508, 821, 1007, 1008, 1263
BpiI GAAGAC 3 cut(s) 90, 150, 590
BpmI CTGGAG 1 cut(s) 407
Bpu1102I GCTNAGC 1 cut(s) 650
Bpu14I TTCGAA 1 cut(s) 603
BpuEI CTTGAG 2 cut(s) 442, 1025
BpuMI CCSGG 2 cut(s) 1007, 1008
BsaBI GATNNNNATC 1 cut(s) 1280
BsaWI WCCGGW 2 cut(s) 643, 721
BsaXI ACNNNNNCTCC 6 cut(s) 688, 718, 836, 866, 1295, 1325
Bsc4I CCNNNNNNNGG 4 cut(s) 154, 670, 923, 924
Bse1I ACTGG 1 cut(s) 165
Bse3DI GCAATG 1 cut(s) 963
Bse8I GATNNNNATC 1 cut(s) 1280
BseBI CCWGG 5 cut(s) 20, 139, 508, 821, 1263
BseGI GGATG 3 cut(s) 402, 981, 1281
BseJI GATNNNNATC 1 cut(s) 1280
BseLI CCNNNNNNNGG 4 cut(s) 154, 670, 923, 924
BseMI GCAATG 1 cut(s) 963
BseMII CTCAG 4 cut(s) 352, 664, 897, 1092
BseNI ACTGG 1 cut(s) 165
BseRI GAGGAG 1 cut(s) 1250
BseSI GKGCMC 1 cut(s) 368
BseXI GCAGC 8 cut(s) 112, 118, 140, 146, 365, 447, 1039, 1221
BseYI CCCAGC 3 cut(s) 148, 277, 1250
BsgI GTGCAG 2 cut(s) 367, 1126
Bsh1236I CGCG 2 cut(s) 155, 291
Bsh1285I CGRYCG 2 cut(s) 289, 1284
BshFI GGCC 4 cut(s) 218, 374, 663, 1046
BshNI GGYRCC 1 cut(s) 281
BsiEI CGRYCG 2 cut(s) 289, 1284
BsiHKAI GWGCWC 8 cut(s) 66, 172, 192, 333, 447, 651, 903, 945
BsiHKCI CYCGRG 3 cut(s) 245, 359, 1006
BsiSI CCGG 4 cut(s) 270, 644, 722, 1007
BslFI GGGAC 5 cut(s) 478, 490, 855, 1111, 1309
BslI CCNNNNNNNGG 4 cut(s) 154, 670, 923, 924
BsmAI GTCTC 1 cut(s) 242
BsmFI GGGAC 5 cut(s) 478, 490, 855, 1111, 1309
BsmI GAATGC 3 cut(s) 571, 886, 1347
BsnI GGCC 4 cut(s) 218, 374, 663, 1046
BsoBI CYCGRG 3 cut(s) 245, 359, 1006
Bsp119I TTCGAA 1 cut(s) 603
Bsp143I GATC 3 cut(s) 838, 1254, 1281
Bsp1720I GCTNAGC 1 cut(s) 650
Bsp19I CCATGG 1 cut(s) 708
BspANI GGCC 4 cut(s) 218, 374, 663, 1046
BspCNI CTCAG 4 cut(s) 351, 663, 898, 1093
BspFNI CGCG 2 cut(s) 155, 291
BspHI TCATGA 1 cut(s) 1122
BspLI GGNNCC 8 cut(s) 243, 283, 478, 479, 853, 870, 871, 1316
BspMAI CTGCAG 2 cut(s) 130, 136
BspMI ACCTGC 2 cut(s) 785, 820
BspPI GGATC 2 cut(s) 846, 1262
BspT104I TTCGAA 1 cut(s) 603
BspT107I GGYRCC 1 cut(s) 281
BsrDI GCAATG 1 cut(s) 963
BsrI ACTGG 1 cut(s) 165
BssMI GATC 3 cut(s) 838, 1254, 1281
BssSI CACGAG 3 cut(s) 665, 1031, 1268
BssT1I CCWWGG 6 cut(s) 588, 708, 847, 885, 922, 1171
Bst2BI CACGAG 3 cut(s) 665, 1031, 1268
Bst2UI CCWGG 5 cut(s) 20, 139, 508, 821, 1263
Bst4CI ACNGT 7 cut(s) 194, 235, 328, 466, 631, 682, 1149
Bst6I CTCTTC 2 cut(s) 47, 258
BstBI TTCGAA 1 cut(s) 603
BstC8I GCNNGC 4 cut(s) 172, 275, 514, 817
BstDEI CTNAG 4 cut(s) 338, 650, 906, 1101
BstDSI CCRYGG 2 cut(s) 153, 708
BstF5I GGATG 3 cut(s) 402, 981, 1281
BstFNI CGCG 2 cut(s) 155, 291
BstKTI GATC 3 cut(s) 841, 1257, 1284
BstMAI GTCTC 1 cut(s) 242
BstMBI GATC 3 cut(s) 838, 1254, 1281
BstMCI CGRYCG 2 cut(s) 289, 1284
BstMWI GCNNNNNNNGC 8 cut(s) 131, 134, 161, 660, 890, 1033, 1206, 1215
BstNI CCWGG 5 cut(s) 20, 139, 508, 821, 1263
BstNSI RCATGY 3 cut(s) 208, 516, 613
BstSCI CCNGG 7 cut(s) 18, 137, 506, 819, 1005, 1006, 1261
BstSFI CTRYAG 3 cut(s) 126, 132, 304
BstSLI GKGCMC 1 cut(s) 368
BstUI CGCG 2 cut(s) 155, 291
BstV1I GCAGC 8 cut(s) 112, 118, 140, 146, 365, 447, 1039, 1221
BstV2I GAAGAC 3 cut(s) 90, 150, 590
BstX2I RGATCY 2 cut(s) 838, 1254
BstXI CCANNNNNNTGG 1 cut(s) 182
BstYI RGATCY 2 cut(s) 838, 1254
BsuRI GGCC 4 cut(s) 218, 374, 663, 1046
BtgI CCRYGG 2 cut(s) 153, 708
BtrI CACGTC 1 cut(s) 387
BtsCI GGATG 3 cut(s) 402, 981, 1281
BtsI GCAGTG 2 cut(s) 943, 1225
BtsIMutI CAGTG 3 cut(s) 172, 943, 1225
BveI ACCTGC 2 cut(s) 785, 820
Cac8I GCNNGC 4 cut(s) 172, 275, 514, 817
CciI TCATGA 1 cut(s) 1122
Cfr13I GGNCC 3 cut(s) 477, 809, 869
Cfr42I CCGCGG 1 cut(s) 156
Cfr9I CCCGGG 1 cut(s) 1006
Csp6I GTAC 4 cut(s) 593, 1069, 1177, 1288
CviQI GTAC 4 cut(s) 593, 1069, 1177, 1288
DdeI CTNAG 4 cut(s) 338, 650, 906, 1101
DpnI GATC 3 cut(s) 840, 1256, 1283
DpnII GATC 3 cut(s) 838, 1254, 1281
EaeI YGGCCR 1 cut(s) 661
Eam1104I CTCTTC 2 cut(s) 47, 258
EarI CTCTTC 2 cut(s) 47, 258
EciI GGCGGA 1 cut(s) 211
Ecl136II GAGCTC 2 cut(s) 649, 901
Eco130I CCWWGG 6 cut(s) 588, 708, 847, 885, 922, 1171
Eco147I AGGCCT 1 cut(s) 218
Eco24I GRGCYC 5 cut(s) 246, 651, 903, 1014, 1269
Eco47I GGWCC 3 cut(s) 477, 809, 869
Eco53kI GAGCTC 2 cut(s) 649, 901
Eco88I CYCGRG 3 cut(s) 245, 359, 1006
EcoICRI GAGCTC 2 cut(s) 649, 901
EcoO109I RGGNCCY 2 cut(s) 477, 869
EcoRII CCWGG 5 cut(s) 18, 137, 506, 819, 1261
EcoT14I CCWWGG 6 cut(s) 588, 708, 847, 885, 922, 1171
EcoT38I GRGCYC 5 cut(s) 246, 651, 903, 1014, 1269
ErhI CCWWGG 6 cut(s) 588, 708, 847, 885, 922, 1171
FalI AAGNNNNNCTT 2 cut(s) 974, 1006
FaqI GGGAC 5 cut(s) 478, 490, 855, 1111, 1309
FauI CCCGC 2 cut(s) 431, 1032
FblI GTMKAC 1 cut(s) 468
FokI GGATG 3 cut(s) 409, 988, 1288
FriOI GRGCYC 5 cut(s) 246, 651, 903, 1014, 1269
FspBI CTAG 3 cut(s) 923, 1172, 1338
GsaI CCCAGC 3 cut(s) 152, 281, 1254
GsuI CTGGAG 1 cut(s) 407
HaeIII GGCC 4 cut(s) 218, 374, 663, 1046
HapII CCGG 4 cut(s) 270, 644, 722, 1007
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 4 cut(s) 250, 913, 1126, 1349
HpaII CCGG 4 cut(s) 270, 644, 722, 1007
HphI GGTGA 7 cut(s) 197, 223, 571, 619, 736, 835, 1251
Hpy166II GTNNAC 7 cut(s) 202, 231, 469, 538, 691, 751, 826
Hpy188I TCNGA 1 cut(s) 907
Hpy188III TCNNGA 6 cut(s) 459, 580, 1123, 1203, 1270, 1338
Hpy8I GTNNAC 7 cut(s) 202, 231, 469, 538, 691, 751, 826
Hpy99I CGWCG 5 cut(s) 71, 529, 532, 635, 1019
HpyAV CCTTC 5 cut(s) 599, 664, 931, 1234, 1321
HpyCH4III ACNGT 7 cut(s) 194, 235, 328, 466, 631, 682, 1149
HpyCH4IV ACGT 6 cut(s) 227, 386, 530, 698, 741, 1298
HpyCH4V TGCA 9 cut(s) 128, 134, 384, 516, 794, 815, 1135, 1143, 1345
HpyF10VI GCNNNNNNNGC 8 cut(s) 131, 134, 161, 660, 890, 1033, 1206, 1215
HpyF3I CTNAG 4 cut(s) 338, 650, 906, 1101
HpySE526I ACGT 6 cut(s) 227, 386, 530, 698, 741, 1298
KflI GGGWCCC 2 cut(s) 477, 869
KspI CCGCGG 1 cut(s) 156
Kzo9I GATC 3 cut(s) 838, 1254, 1281
LmnI GCTCC 8 cut(s) 241, 426, 442, 646, 857, 940, 1303, 1314
Lsp1109I GCAGC 8 cut(s) 112, 118, 140, 146, 365, 447, 1039, 1221
MaeI CTAG 3 cut(s) 923, 1172, 1338
MaeII ACGT 6 cut(s) 227, 386, 530, 698, 741, 1298
MaeIII GTNAC 1 cut(s) 676
MalI GATC 3 cut(s) 840, 1256, 1283
MboI GATC 3 cut(s) 838, 1254, 1281
MboII GAAGA 9 cut(s) 64, 95, 155, 245, 289, 493, 595, 991, 994
MflI RGATCY 2 cut(s) 838, 1254
MluCI AATT 3 cut(s) 999, 1319, 1332
MlyI GAGTC 2 cut(s) 244, 1358
MmeI TCCRAC 1 cut(s) 1281
MseI TTAA 2 cut(s) 318, 992
MslI CAYNNNNRTG 5 cut(s) 195, 236, 554, 683, 1163
MspA1I CMGCKG 3 cut(s) 131, 155, 438
MspI CCGG 4 cut(s) 270, 644, 722, 1007
MspR9I CCNGG 7 cut(s) 20, 139, 508, 821, 1007, 1008, 1263
Mva1269I GAATGC 3 cut(s) 571, 886, 1347
MvaI CCWGG 5 cut(s) 20, 139, 508, 821, 1263
MvnI CGCG 2 cut(s) 155, 291
MwoI GCNNNNNNNGC 8 cut(s) 131, 134, 161, 660, 890, 1033, 1206, 1215
NciI CCSGG 2 cut(s) 1007, 1008
NcoI CCATGG 1 cut(s) 708
NdeII GATC 3 cut(s) 838, 1254, 1281
NlaIV GGNNCC 8 cut(s) 243, 283, 478, 479, 853, 870, 871, 1316
NmeAIII GCCGAG 1 cut(s) 101
NmuCI GTSAC 1 cut(s) 676
NspI RCATGY 3 cut(s) 208, 516, 613
NspV TTCGAA 1 cut(s) 603
OliI CACNNNNGTG 2 cut(s) 195, 683
PaeI GCATGC 1 cut(s) 516
PaeR7I CTCGAG 1 cut(s) 359
PagI TCATGA 1 cut(s) 1122
PaqCI CACCTGC 1 cut(s) 785
PasI CCCWGGG 2 cut(s) 19, 507
PceI AGGCCT 1 cut(s) 218
PciI ACATGT 1 cut(s) 204
PctI GAATGC 3 cut(s) 571, 886, 1347
PfeI GAWTC 2 cut(s) 913, 1126
Ple19I CGATCG 1 cut(s) 1284
PleI GAGTC 2 cut(s) 244, 1357
PpsI GAGTC 2 cut(s) 244, 1357
PpuMI RGGWCCY 2 cut(s) 477, 869
PscI ACATGT 1 cut(s) 204
Psp124BI GAGCTC 2 cut(s) 651, 903
Psp5II RGGWCCY 2 cut(s) 477, 869
Psp6I CCWGG 5 cut(s) 18, 137, 506, 819, 1261
PspFI CCCAGC 3 cut(s) 148, 277, 1250
PspGI CCWGG 5 cut(s) 18, 137, 506, 819, 1261
PspN4I GGNNCC 8 cut(s) 243, 283, 478, 479, 853, 870, 871, 1316
PspPI GGNCC 3 cut(s) 477, 809, 869
PspPPI RGGWCCY 2 cut(s) 477, 869
PspXI VCTCGAGB 1 cut(s) 359
PstI CTGCAG 2 cut(s) 130, 136
PsuI RGATCY 2 cut(s) 838, 1254
PvuI CGATCG 1 cut(s) 1284
PvuII CAGCTG 1 cut(s) 131
RsaI GTAC 4 cut(s) 594, 1070, 1178, 1289
RsaNI GTAC 4 cut(s) 593, 1069, 1177, 1288
RseI CAYNNNNRTG 5 cut(s) 195, 236, 554, 683, 1163
SacI GAGCTC 2 cut(s) 651, 903
SacII CCGCGG 1 cut(s) 156
SaqAI TTAA 2 cut(s) 318, 992
Sau3AI GATC 3 cut(s) 838, 1254, 1281
Sau96I GGNCC 3 cut(s) 477, 809, 869
SchI GAGTC 2 cut(s) 244, 1358
ScrFI CCNGG 7 cut(s) 20, 139, 508, 821, 1007, 1008, 1263
SfcI CTRYAG 3 cut(s) 126, 132, 304
Sfr274I CTCGAG 1 cut(s) 359
Sfr303I CCGCGG 1 cut(s) 156
SfuI TTCGAA 1 cut(s) 603
SgrBI CCGCGG 1 cut(s) 156
SinI GGWCC 3 cut(s) 477, 809, 869
SlaI CTCGAG 1 cut(s) 359
SmaI CCCGGG 1 cut(s) 1008
SmiMI CAYNNNNRTG 5 cut(s) 195, 236, 554, 683, 1163
SmlI CTYRAG 3 cut(s) 359, 457, 1040
SmoI CTYRAG 3 cut(s) 359, 457, 1040
SphI GCATGC 1 cut(s) 516
Sse9I AATT 3 cut(s) 999, 1319, 1332
SseBI AGGCCT 1 cut(s) 218
SspMI CTAG 3 cut(s) 923, 1172, 1338
SstI GAGCTC 2 cut(s) 651, 903
StuI AGGCCT 1 cut(s) 218
StyD4I CCNGG 7 cut(s) 18, 137, 506, 819, 1005, 1006, 1261
StyI CCWWGG 6 cut(s) 588, 708, 847, 885, 922, 1171
TaaI ACNGT 7 cut(s) 194, 235, 328, 466, 631, 682, 1149
TaiI ACGT 6 cut(s) 230, 389, 533, 701, 744, 1301
TaqI TCGA 6 cut(s) 209, 360, 603, 614, 1014, 1280
TasI AATT 3 cut(s) 999, 1319, 1332
TauI GCSGC 6 cut(s) 98, 155, 158, 312, 377, 777
TfiI GAWTC 2 cut(s) 913, 1126
Tru1I TTAA 2 cut(s) 318, 992
Tru9I TTAA 2 cut(s) 318, 992
TscAI CASTG 3 cut(s) 172, 950, 1225
TseFI GTSAC 1 cut(s) 676
TseI GCWGC 8 cut(s) 125, 128, 131, 134, 353, 435, 1027, 1209
Tsp45I GTSAC 1 cut(s) 676
TspDTI ATGAA 8 cut(s) 289, 893, 950, 992, 1139, 1147, 1197, 1241
TspGWI ACGGA 3 cut(s) 403, 486, 1086
TspMI CCCGGG 1 cut(s) 1006
TspRI CASTG 3 cut(s) 172, 950, 1225
VpaK11BI GGWCC 3 cut(s) 477, 809, 869
XbaI TCTAGA 1 cut(s) 1337
XceI RCATGY 3 cut(s) 208, 516, 613
XhoI CTCGAG 1 cut(s) 359
XmaI CCCGGG 1 cut(s) 1006
XmaJI CCTAGG 2 cut(s) 922, 1171
XmiI GTMKAC 1 cut(s) 468
XspI CTAG 3 cut(s) 923, 1172, 1338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.