FvH4_5g10890

Methionine gamma-lyase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012000
Physical Location & Seq
Reverse (-)
6169787 .. 6173788
4002 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g10890.t1

Sequence Viewer

Length: 1368 bp
ATGGCTGAGACCCTTCTCCCCACCAACAACAACACCAAGAAGAGAACCGAGCACGACGACATGGACGGACAAGACTACGCCGCTTCCGCCGCCAAGAAATCCATGTTCTTGCCCACAGCCGCGCCCGCGACGTGGGAGGACCCCGCCATGGCATTAGCCAGTGCTCGCCATGAGTTTGGCGAGCACGGTGGGGTTAACATGTCCATCGAGGCCTCGGCCACGTTCACCGTCATGGAGCCGGAGACCCTCCAAAAGATGTTTTCCGGCGAGCTCGGCGCCGATCGTGACTTCTTCATCTACAGCCGTCACTTTAACCCCACTGTCCTTAACCTCAGCCGTCAGATGGCTGCCCTCGAGGGCACAGAGGCCGCCTACTGCACCTCCAGCGGCATGTCCGCCATCTCCTCCGTCCTTCTCCAACTCGTCAGCAGCGGCGACCACATCGTCGCCTCTAGAACCCTCTACGGTGGAACGCACGCCCTCCTCACCCACTTTTTCCCCAGAGCCTGCAACATAACCACAACGTTCGTCGACATAAACGACCTTGACATGGTTAGGAATGCGATCGAGGTCGGAAAGACTAAAGTTGTATATTTCGAGGCAATGTCTAACCCTACTCTCACCGTCGCTAACATACCGGAGCTCAGCCGGATTAGTCACGAGAAAGGGGTCACCGTCGTGGTAGACAACACCTTCTCTCCCATGGTTCTCTCCCCGGTTAAGCTCGGCGCTGACGTTGTTGTCCACAGCATATCCAAGTTCATCAGCGGCGGTGCTGACATCATTGCAGGTGTTGTGTGTGGACCTGCAAGCTTGGTGAACTCGATGATGGACCTTCACCAAGGTGCCCTAATGCTTCTAGGACCCACCATGAATGCCAAGGTTGCATTTGAGCTCGCTGAGAGGGTCCCTCACTTGGGTCTGAGAATGAAGGAGCACTGCAACAGGGCCATGGTGTATGCCACAAGGATGAAGAAGATGGGACTCAAGGTCATATACCCTGGCCTCGAAGAGCATCCCCAGCACGAGCTTCTCAAGTCCATGATCAACAAGGATTATGGCTATGGTGGACTTCTCTGCCTCGACATGGGGACTGAGGAGAGAGCCAATAGGTTGATGAACCTGTTGCAGAACTACACGCAGTTTGGGTTCATGGCTGTGAGCTTGGGGTACTATGAAACCCTGATGTCCTGCTCTGGAAGTAGCACAAGCAGTGAAATGAACAATGAGGAGAAGGAGTTGGCTGGGATTTCACCTGGGCTGGTTAGAATGTCAATTGGTTACATCGGAACTTTGGAGCAGAAGTGGAGCCAGTTGGAGAAGGCACTGACTAGAATGCATGATTCTGGTTTGTTTGGCAAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00450 map00270 map00450
Pfam Domains
Protein Families
Cys_Met_Meta_PP

Protein Analysis

456

Amino Acids

49.67

Weight (kDa)

5.93

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cys_Met_Meta_PP PF01053 60 - 427 4.4e-85 Cys/Met metabolism PLP-dependent enzyme
Aminotran_1_2 PF00155 113 - 332 2.4e-10 Aminotransferase class I and II
Aminotran_5 PF00266 127 - 293 1.3e-07 Aminotransferase class-V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012000)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64660
fragaria_vesca FvH4_5g10890 FvH4_5g10890 FvH4_5g10890
malus_domestica MD06G1118700.v1.1 MD14G1126800.v1.1
prunus_persica Prupe.5G129800_v2.0.a1
pyrus_communis pycom06g11020 pycom14g11140
rosa_chinensis RchiOBHm_Chr7g0186971
rosa_laevigata RLG00000004805
rosa_multiflora Rmu_sc0007656.1_g000001
rosa_roxburghii Rroxscaffold_3G00267720
rosa_rugosa Rorug06G0478300
rosa_samantha Rh7AG083900 Rh7BG079000 Rh7CG084900 Rh7DG085800
rosa_wichuraiana Rw7G007380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 779
AasI GACNNNNNNGTC 2 cut(s) 443, 740
AbsI CCTCGAGG 1 cut(s) 353
Acc36I ACCTGC 2 cut(s) 779, 814
AccB1I GGYRCC 2 cut(s) 275, 845
AccI GTMKAC 2 cut(s) 531, 684
AccII CGCG 2 cut(s) 122, 128
AclI AACGTT 1 cut(s) 524
AcoI YGGCCR 1 cut(s) 216
AcyI GRCGYC 1 cut(s) 276
AfaI GTAC 1 cut(s) 1172
AfiI CCNNNNNNNGG 7 cut(s) 132, 148, 343, 550, 916, 917, 1087
AflIII ACRYGT 1 cut(s) 198
AhdI GACNNNNNGTC 1 cut(s) 989
AjiI CACGTC 1 cut(s) 132
AjnI CCWGG 2 cut(s) 1000, 1255
AleI CACNNNNGTG 2 cut(s) 677, 843
AluBI AGCT 7 cut(s) 271, 643, 724, 813, 895, 1030, 1164
AluI AGCT 7 cut(s) 271, 643, 724, 813, 895, 1030, 1164
Alw21I GWGCWC 7 cut(s) 54, 166, 186, 273, 645, 897, 939
Alw26I GTCTC 2 cut(s) 2, 236
AlwNI CAGNNNCTG 1 cut(s) 507
Ama87I CYCGRG 1 cut(s) 353
AoxI GGCC 5 cut(s) 210, 216, 366, 948, 1003
ApeKI GCWGC 2 cut(s) 347, 429
AspLEI GCGC 3 cut(s) 124, 278, 731
AspS9I GGNCC 6 cut(s) 139, 803, 832, 863, 907, 948
AsuC2I CCSGG 1 cut(s) 716
AsuHPI GGTGA 7 cut(s) 217, 478, 613, 664, 829, 830, 1245
AvaI CYCGRG 1 cut(s) 353
AvaII GGWCC 5 cut(s) 139, 803, 832, 863, 907
BaeGI GKGCMC 2 cut(s) 362, 850
BanI GGYRCC 2 cut(s) 275, 845
BanII GRGCYC 3 cut(s) 273, 645, 897
BauI CACGAG 2 cut(s) 659, 1025
Bbv12I GWGCWC 7 cut(s) 54, 166, 186, 273, 645, 897, 939
BbvCI CCTCAGC 1 cut(s) 332
BbvI GCAGC 2 cut(s) 334, 441
BccI CCATC 5 cut(s) 212, 337, 407, 823, 973
BceAI ACGGC 2 cut(s) 288, 321
BciT130I CCWGG 2 cut(s) 1002, 1257
BclI TGATCA 1 cut(s) 1044
BcnI CCSGG 1 cut(s) 716
BcoDI GTCTC 2 cut(s) 2, 236
BfaI CTAG 3 cut(s) 453, 860, 1332
BfmI CTRYAG 1 cut(s) 298
BfoI RGCGCY 2 cut(s) 279, 732
BfuAI ACCTGC 2 cut(s) 779, 814
BisI GCNGC 9 cut(s) 81, 90, 120, 348, 369, 388, 430, 433, 769
BlpI GCTNAGC 1 cut(s) 644
BlsI GCNGC 9 cut(s) 82, 91, 121, 349, 370, 389, 431, 434, 770
Bme1390I CCNGG 3 cut(s) 716, 1002, 1257
Bme18I GGWCC 5 cut(s) 139, 803, 832, 863, 907
BmeRI GACNNNNNGTC 1 cut(s) 989
BmeT110I CYCGRG 1 cut(s) 353
BmgBI CACGTC 1 cut(s) 132
BmgT120I GGNCC 6 cut(s) 139, 803, 832, 863, 907, 948
BmiI GGNNCC 8 cut(s) 141, 237, 277, 847, 865, 908, 909, 1310
BmrFI CCNGG 3 cut(s) 716, 1002, 1257
BmsI GCATC 1 cut(s) 1024
BplI GAGNNNNNCTC 2 cut(s) 895, 927
BpmI CTGGAG 1 cut(s) 367
Bpu10I CCTNAGC 1 cut(s) 332
Bpu1102I GCTNAGC 1 cut(s) 644
BpuEI CTTGAG 2 cut(s) 971, 1019
BpuMI CCSGG 1 cut(s) 716
BsaHI GRCGYC 1 cut(s) 276
BsaI GGTCTC 2 cut(s) 2, 236
BsaJI CCNNGG 9 cut(s) 147, 213, 702, 714, 841, 879, 951, 1000, 1256
BsaWI WCCGGW 1 cut(s) 637
BsaXI ACNNNNNCTCC 8 cut(s) 365, 395, 682, 712, 1223, 1253, 1289, 1319
Bsc4I CCNNNNNNNGG 7 cut(s) 132, 148, 343, 550, 916, 917, 1087
Bse1I ACTGG 2 cut(s) 159, 1312
Bse3DI GCAATG 2 cut(s) 609, 783
BseBI CCWGG 2 cut(s) 1002, 1257
BseDI CCNNGG 9 cut(s) 147, 213, 702, 714, 841, 879, 951, 1000, 1256
BseGI GGATG 2 cut(s) 975, 1015
BseLI CCNNNNNNNGG 7 cut(s) 132, 148, 343, 550, 916, 917, 1087
BseMI GCAATG 2 cut(s) 609, 783
BseMII CTCAG 5 cut(s) 346, 658, 891, 914, 1086
BseNI ACTGG 2 cut(s) 159, 1312
BseRI GAGGAG 4 cut(s) 394, 473, 1112, 1244
BseSI GKGCMC 2 cut(s) 362, 850
BseXI GCAGC 2 cut(s) 334, 441
BseYI CCCAGC 2 cut(s) 1020, 1244
BsgI GTGCAG 1 cut(s) 361
Bsh1236I CGCG 2 cut(s) 122, 128
Bsh1285I CGRYCG 2 cut(s) 283, 567
BshFI GGCC 5 cut(s) 212, 218, 368, 950, 1005
BshNI GGYRCC 2 cut(s) 275, 845
BsiEI CGRYCG 2 cut(s) 283, 567
BsiHKAI GWGCWC 7 cut(s) 54, 166, 186, 273, 645, 897, 939
BsiHKCI CYCGRG 1 cut(s) 353
BsiSI CCGG 5 cut(s) 239, 264, 638, 649, 716
BslFI GGGAC 3 cut(s) 893, 996, 1105
BslI CCNNNNNNNGG 7 cut(s) 132, 148, 343, 550, 916, 917, 1087
BsmAI GTCTC 2 cut(s) 2, 236
BsmFI GGGAC 3 cut(s) 893, 996, 1105
BsmI GAATGC 3 cut(s) 565, 880, 1341
BsnI GGCC 5 cut(s) 212, 218, 368, 950, 1005
Bso31I GGTCTC 2 cut(s) 2, 236
BsoBI CYCGRG 1 cut(s) 353
Bsp1286I GDGCHC 9 cut(s) 54, 166, 186, 273, 362, 645, 850, 897, 939
Bsp143I GATC 3 cut(s) 280, 564, 1044
Bsp1720I GCTNAGC 1 cut(s) 644
Bsp19I CCATGG 3 cut(s) 147, 702, 951
BspANI GGCC 5 cut(s) 212, 218, 368, 950, 1005
BspCNI CTCAG 5 cut(s) 345, 657, 892, 915, 1087
BspFNI CGCG 2 cut(s) 122, 128
BspLI GGNNCC 8 cut(s) 141, 237, 277, 847, 865, 908, 909, 1310
BspMI ACCTGC 2 cut(s) 779, 814
BspQI GCTCTTC 1 cut(s) 1005
BspT107I GGYRCC 2 cut(s) 275, 845
BspTNI GGTCTC 2 cut(s) 2, 236
BsrDI GCAATG 2 cut(s) 609, 783
BsrI ACTGG 2 cut(s) 159, 1312
BssECI CCNNGG 9 cut(s) 147, 213, 702, 714, 841, 879, 951, 1000, 1256
BssMI GATC 3 cut(s) 280, 564, 1044
BssNI GRCGYC 1 cut(s) 276
BssSI CACGAG 2 cut(s) 659, 1025
BssT1I CCWWGG 5 cut(s) 147, 702, 841, 879, 951
Bst2BI CACGAG 2 cut(s) 659, 1025
Bst2UI CCWGG 2 cut(s) 1002, 1257
Bst4CI ACNGT 6 cut(s) 188, 229, 322, 467, 625, 676
Bst6I CTCTTC 2 cut(s) 35, 1005
BstACI GRCGYC 1 cut(s) 276
BstC8I GCNNGC 8 cut(s) 126, 166, 182, 269, 477, 508, 811, 897
BstDEI CTNAG 6 cut(s) 6, 332, 644, 900, 923, 1095
BstDSI CCRYGG 3 cut(s) 147, 702, 951
BstEII GGTNACC 1 cut(s) 670
BstF5I GGATG 2 cut(s) 975, 1015
BstFNI CGCG 2 cut(s) 122, 128
BstH2I RGCGCY 2 cut(s) 279, 732
BstHHI GCGC 3 cut(s) 124, 278, 731
BstKTI GATC 3 cut(s) 283, 567, 1047
BstMAI GTCTC 2 cut(s) 2, 236
BstMBI GATC 3 cut(s) 280, 564, 1044
BstMCI CGRYCG 2 cut(s) 283, 567
BstMWI GCNNNNNNNGC 7 cut(s) 86, 89, 125, 273, 384, 884, 1021
BstNI CCWGG 2 cut(s) 1002, 1257
BstNSI RCATGY 2 cut(s) 202, 394
BstPI GGTNACC 1 cut(s) 670
BstSCI CCNGG 3 cut(s) 714, 1000, 1255
BstSFI CTRYAG 1 cut(s) 298
BstSLI GKGCMC 2 cut(s) 362, 850
BstUI CGCG 2 cut(s) 122, 128
BstV1I GCAGC 2 cut(s) 334, 441
BstXI CCANNNNNNTGG 1 cut(s) 176
BsuRI GGCC 5 cut(s) 212, 218, 368, 950, 1005
BtgI CCRYGG 3 cut(s) 147, 702, 951
BtrI CACGTC 1 cut(s) 132
BtsCI GGATG 2 cut(s) 975, 1015
BtsI GCAGTG 2 cut(s) 937, 1219
BtsIMutI CAGTG 5 cut(s) 166, 318, 937, 1219, 1325
BveI ACCTGC 2 cut(s) 779, 814
Cac8I GCNNGC 8 cut(s) 126, 166, 182, 269, 477, 508, 811, 897
CaiI CAGNNNCTG 1 cut(s) 507
CfoI GCGC 3 cut(s) 124, 278, 731
Cfr13I GGNCC 6 cut(s) 139, 803, 832, 863, 907, 948
Csp6I GTAC 1 cut(s) 1171
CviQI GTAC 1 cut(s) 1171
DdeI CTNAG 6 cut(s) 6, 332, 644, 900, 923, 1095
DinI GGCGCC 1 cut(s) 277
DpnI GATC 3 cut(s) 282, 566, 1046
DpnII GATC 3 cut(s) 280, 564, 1044
DrdI GACNNNNNNGTC 2 cut(s) 443, 740
DriI GACNNNNNGTC 1 cut(s) 989
DseDI GACNNNNNNGTC 2 cut(s) 443, 740
EaeI YGGCCR 1 cut(s) 216
Eam1104I CTCTTC 2 cut(s) 35, 1005
Eam1105I GACNNNNNGTC 1 cut(s) 989
EarI CTCTTC 2 cut(s) 35, 1005
EciI GGCGGA 2 cut(s) 76, 385
Ecl136II GAGCTC 3 cut(s) 271, 643, 895
Eco130I CCWWGG 5 cut(s) 147, 702, 841, 879, 951
Eco147I AGGCCT 1 cut(s) 212
Eco24I GRGCYC 3 cut(s) 273, 645, 897
Eco31I GGTCTC 2 cut(s) 2, 236
Eco47I GGWCC 5 cut(s) 139, 803, 832, 863, 907
Eco53kI GAGCTC 3 cut(s) 271, 643, 895
Eco88I CYCGRG 1 cut(s) 353
Eco91I GGTNACC 1 cut(s) 670
EcoICRI GAGCTC 3 cut(s) 271, 643, 895
EcoO109I RGGNCCY 3 cut(s) 139, 863, 907
EcoO65I GGTNACC 1 cut(s) 670
EcoRII CCWGG 2 cut(s) 1000, 1255
EcoT14I CCWWGG 5 cut(s) 147, 702, 841, 879, 951
EcoT22I ATGCAT 1 cut(s) 1341
EcoT38I GRGCYC 3 cut(s) 273, 645, 897
EgeI GGCGCC 1 cut(s) 277
EheI GGCGCC 1 cut(s) 277
ErhI CCWWGG 5 cut(s) 147, 702, 841, 879, 951
FaqI GGGAC 3 cut(s) 893, 996, 1105
FauI CCCGC 2 cut(s) 133, 151
FbaI TGATCA 1 cut(s) 1044
FblI GTMKAC 2 cut(s) 531, 684
Fnu4HI GCNGC 9 cut(s) 81, 90, 120, 348, 369, 388, 430, 433, 769
FokI GGATG 2 cut(s) 982, 1002
FriOI GRGCYC 3 cut(s) 273, 645, 897
Fsp4HI GCNGC 9 cut(s) 81, 90, 120, 348, 369, 388, 430, 433, 769
FspBI CTAG 3 cut(s) 453, 860, 1332
GlaI GCGC 3 cut(s) 123, 277, 730
GluI GCNGC 9 cut(s) 81, 90, 120, 348, 369, 388, 430, 433, 769
GsaI CCCAGC 2 cut(s) 1024, 1248
GsuI CTGGAG 1 cut(s) 367
HaeII RGCGCY 2 cut(s) 279, 732
HaeIII GGCC 5 cut(s) 212, 218, 368, 950, 1005
HapII CCGG 5 cut(s) 239, 264, 638, 649, 716
HhaI GCGC 3 cut(s) 124, 278, 731
Hin1I GRCGYC 1 cut(s) 276
Hin6I GCGC 3 cut(s) 122, 276, 729
HinP1I GCGC 3 cut(s) 122, 276, 729
HincII GTYRAC 2 cut(s) 196, 532
HindII GTYRAC 2 cut(s) 196, 532
HindIII AAGCTT 1 cut(s) 811
HinfI GANTC 2 cut(s) 984, 1343
HpaI GTTAAC 1 cut(s) 196
HpaII CCGG 5 cut(s) 239, 264, 638, 649, 716
HphI GGTGA 7 cut(s) 217, 478, 613, 664, 829, 830, 1245
Hpy166II GTNNAC 8 cut(s) 196, 225, 532, 685, 745, 803, 820, 1070
Hpy188I TCNGA 4 cut(s) 342, 575, 924, 1289
Hpy188III TCNNGA 4 cut(s) 284, 453, 659, 1197
Hpy8I GTNNAC 8 cut(s) 196, 225, 532, 685, 745, 803, 820, 1070
Hpy99I CGWCG 6 cut(s) 59, 133, 449, 533, 629, 680
HpyAV CCTTC 7 cut(s) 23, 422, 703, 845, 925, 1228, 1315
HpyCH4III ACNGT 6 cut(s) 188, 229, 322, 467, 625, 676
HpyCH4IV ACGT 4 cut(s) 131, 221, 524, 735
HpyCH4V TGCA 8 cut(s) 378, 510, 788, 809, 887, 942, 1129, 1339
HpyF10VI GCNNNNNNNGC 7 cut(s) 86, 89, 125, 273, 384, 884, 1021
HpyF3I CTNAG 6 cut(s) 6, 332, 644, 900, 923, 1095
HpySE526I ACGT 4 cut(s) 131, 221, 524, 735
Hsp92I GRCGYC 1 cut(s) 276
HspAI GCGC 3 cut(s) 122, 276, 729
KasI GGCGCC 1 cut(s) 275
KflI GGGWCCC 1 cut(s) 907
Ksp22I TGATCA 1 cut(s) 1044
KspAI GTTAAC 1 cut(s) 196
Kzo9I GATC 3 cut(s) 280, 564, 1044
LguI GCTCTTC 1 cut(s) 1005
LmnI GCTCC 5 cut(s) 235, 640, 934, 1297, 1308
Lsp1109I GCAGC 2 cut(s) 334, 441
LweI GCATC 1 cut(s) 1024
MaeI CTAG 3 cut(s) 453, 860, 1332
MaeII ACGT 4 cut(s) 131, 221, 524, 735
MaeIII GTNAC 5 cut(s) 284, 305, 656, 670, 1280
MalI GATC 3 cut(s) 282, 566, 1046
MboI GATC 3 cut(s) 280, 564, 1044
MboII GAAGA 5 cut(s) 52, 283, 985, 988, 1022
MfeI CAATTG 1 cut(s) 1275
MhlI GDGCHC 9 cut(s) 54, 166, 186, 273, 362, 645, 850, 897, 939
MluCI AATT 1 cut(s) 1275
Mly113I GGCGCC 1 cut(s) 276
MlyI GAGTC 1 cut(s) 978
MmeI TCCRAC 3 cut(s) 442, 553, 1296
Mph1103I ATGCAT 1 cut(s) 1341
MseI TTAA 4 cut(s) 195, 312, 327, 720
MslI CAYNNNNRTG 5 cut(s) 230, 677, 843, 968, 1157
MspA1I CMGCKG 3 cut(s) 387, 432, 768
MspI CCGG 5 cut(s) 239, 264, 638, 649, 716
MspR9I CCNGG 3 cut(s) 716, 1002, 1257
MunI CAATTG 1 cut(s) 1275
Mva1269I GAATGC 3 cut(s) 565, 880, 1341
MvaI CCWGG 2 cut(s) 1002, 1257
MvnI CGCG 2 cut(s) 122, 128
MwoI GCNNNNNNNGC 7 cut(s) 86, 89, 125, 273, 384, 884, 1021
NarI GGCGCC 1 cut(s) 276
NciI CCSGG 1 cut(s) 716
NcoI CCATGG 3 cut(s) 147, 702, 951
NdeII GATC 3 cut(s) 280, 564, 1044
NlaIV GGNNCC 8 cut(s) 141, 237, 277, 847, 865, 908, 909, 1310
NmeAIII GCCGAG 3 cut(s) 194, 252, 705
NmuCI GTSAC 4 cut(s) 284, 305, 656, 670
NsiI ATGCAT 1 cut(s) 1341
NspI RCATGY 2 cut(s) 202, 394
OliI CACNNNNGTG 2 cut(s) 677, 843
PaeR7I CTCGAG 1 cut(s) 353
PaqCI CACCTGC 1 cut(s) 779
PceI AGGCCT 1 cut(s) 212
PciI ACATGT 1 cut(s) 198
PciSI GCTCTTC 1 cut(s) 1005
PcsI WCGNNNNNNNCGW 2 cut(s) 537, 732
PctI GAATGC 3 cut(s) 565, 880, 1341
PfeI GAWTC 1 cut(s) 1343
PkrI GCNGC 9 cut(s) 82, 91, 121, 349, 370, 389, 431, 434, 770
Ple19I CGATCG 2 cut(s) 283, 567
PleI GAGTC 1 cut(s) 978
PluTI GGCGCC 1 cut(s) 279
PpsI GAGTC 1 cut(s) 978
PpuMI RGGWCCY 3 cut(s) 139, 863, 907
PscI ACATGT 1 cut(s) 198
Psp124BI GAGCTC 3 cut(s) 273, 645, 897
Psp1406I AACGTT 1 cut(s) 524
Psp5II RGGWCCY 3 cut(s) 139, 863, 907
Psp6I CCWGG 2 cut(s) 1000, 1255
PspEI GGTNACC 1 cut(s) 670
PspFI CCCAGC 2 cut(s) 1020, 1244
PspGI CCWGG 2 cut(s) 1000, 1255
PspN4I GGNNCC 8 cut(s) 141, 237, 277, 847, 865, 908, 909, 1310
PspPI GGNCC 6 cut(s) 139, 803, 832, 863, 907, 948
PspPPI RGGWCCY 3 cut(s) 139, 863, 907
PspXI VCTCGAGB 1 cut(s) 353
PstNI CAGNNNCTG 1 cut(s) 507
PvuI CGATCG 2 cut(s) 283, 567
RsaI GTAC 1 cut(s) 1172
RsaNI GTAC 1 cut(s) 1171
RseI CAYNNNNRTG 5 cut(s) 230, 677, 843, 968, 1157
SacI GAGCTC 3 cut(s) 273, 645, 897
SalI GTCGAC 1 cut(s) 530
SapI GCTCTTC 1 cut(s) 1005
SaqAI TTAA 4 cut(s) 195, 312, 327, 720
SatI GCNGC 9 cut(s) 81, 90, 120, 348, 369, 388, 430, 433, 769
Sau3AI GATC 3 cut(s) 280, 564, 1044
Sau96I GGNCC 6 cut(s) 139, 803, 832, 863, 907, 948
SchI GAGTC 1 cut(s) 978
ScrFI CCNGG 3 cut(s) 716, 1002, 1257
SduI GDGCHC 9 cut(s) 54, 166, 186, 273, 362, 645, 850, 897, 939
SfaNI GCATC 1 cut(s) 1024
SfcI CTRYAG 1 cut(s) 298
SfoI GGCGCC 1 cut(s) 277
Sfr274I CTCGAG 1 cut(s) 353
SinI GGWCC 5 cut(s) 139, 803, 832, 863, 907
SlaI CTCGAG 1 cut(s) 353
SmiMI CAYNNNNRTG 5 cut(s) 230, 677, 843, 968, 1157
SmlI CTYRAG 3 cut(s) 353, 986, 1034
SmoI CTYRAG 3 cut(s) 353, 986, 1034
Sse9I AATT 1 cut(s) 1275
SseBI AGGCCT 1 cut(s) 212
SspDI GGCGCC 1 cut(s) 275
SspMI CTAG 3 cut(s) 453, 860, 1332
SstI GAGCTC 3 cut(s) 273, 645, 897
StuI AGGCCT 1 cut(s) 212
StyD4I CCNGG 3 cut(s) 714, 1000, 1255
StyI CCWWGG 5 cut(s) 147, 702, 841, 879, 951
TaaI ACNGT 6 cut(s) 188, 229, 322, 467, 625, 676
TaiI ACGT 4 cut(s) 134, 224, 527, 738
TaqI TCGA 8 cut(s) 207, 354, 531, 567, 597, 824, 1008, 1083
TasI AATT 1 cut(s) 1275
TauI GCSGC 7 cut(s) 83, 92, 122, 371, 390, 435, 771
TfiI GAWTC 1 cut(s) 1343
Tru1I TTAA 4 cut(s) 195, 312, 327, 720
Tru9I TTAA 4 cut(s) 195, 312, 327, 720
TscAI CASTG 5 cut(s) 166, 325, 944, 1219, 1332
TseFI GTSAC 4 cut(s) 284, 305, 656, 670
TseI GCWGC 2 cut(s) 347, 429
Tsp45I GTSAC 4 cut(s) 284, 305, 656, 670
TspDTI ATGAA 9 cut(s) 283, 751, 887, 944, 986, 1133, 1141, 1191, 1235
TspGWI ACGGA 2 cut(s) 81, 397
TspRI CASTG 5 cut(s) 166, 325, 944, 1219, 1332
VpaK11BI GGWCC 5 cut(s) 139, 803, 832, 863, 907
XbaI TCTAGA 1 cut(s) 452
XceI RCATGY 2 cut(s) 202, 394
XhoI CTCGAG 1 cut(s) 353
XmiI GTMKAC 2 cut(s) 531, 684
XspI CTAG 3 cut(s) 453, 860, 1332
Zsp2I ATGCAT 1 cut(s) 1341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.