Rmu_sc0007656.1_g000001

Methionine gamma-lyase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007656.1
OG0012000
Physical Location & Seq
Forward (+)
1586 .. 3989
2404 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0007656.1_g000001.1.cds

Sequence Viewer

Length: 1386 bp
atggcggagaccagaccccaagcctttgttcttcccaccaaaaacaacagcagcaagaagagaacggagcacgacggcatggacggccaagactacgccgcttccgccgccaagaagtcgatgttcttgcccacggcggcgtccgcggggtgggaggaccccgctatggcattagccaatgctcgccacgagtttggcgagcacggcggggtcaacatgtccatcgaggcctcggccactttcaccgtcatggagccggagaccctccgcaagatgttttccggcgagctcggcgccgaccgcgacttcttcatctacagccgtcactttaaccccacggtccttaacctcagccgtcagatggcggcccttgagggcaccgaggccgcctactgcacctccagcggcatgtccgccatctcctccgtcctgctgcaactcgtcagcagcggcgaccacatcgtcgcttctagaaccctgtacggcgggacccacgccctcctcactcacttcttccccagagcctgcaacataaccacaacgttcgttgacataaacgacctcgacatggtcaggaatgccatcgaggtgggaaagactaaggttctctatttcgagggaatgtctaacccaactctcaccgtggctaacataccggagctcagccgaattgggcacgagaaaggcgtcaccgtcgtcgtggacaacaccttctcacccatggttgtctccccggttaagctcggcgctgacgtggttgtccacagcatatccaagttcatcagcggcggtgctgacatcattgcaggtgttgtgtgtggacctgcaagcttggtgaactcaatgatggaccttcaccaaggttccctaatgcttcttggccccaccatgaatgcgaaggtagcatttgagctctctgagaggatacctcacttgggtttgagaatgaaggagcactgcaacagggcattggtgtatgccacaaggatgaagaagatgggactaaaagtcatatacccaggcctcgaagaccatccccagcacgagcttctcaagtccatgatcaacaaggattatggctatggtggacttctttgcgtcgacatggggactgaggagagagccaataggttgatgaatctgttgcagaactacacacagtttgggttcatggctgtgagcttggggtactatgaaaccctaatgtcctgctctggaagcagcacaagcagtgaaatgagcaatgaggagaaggagttggctgggatttcacctgggctggttagaatgtcaattggttatattggaactttggagcagaggtggagccagtttgagaaggcactcaatagaatgcaggattctggtttgtttggcaagaagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00450 map00270 map00450
Pfam Domains
Protein Families
Cys_Met_Meta_PP

Protein Analysis

461

Amino Acids

50.28

Weight (kDa)

6.28

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012000)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64660
fragaria_vesca FvH4_5g10890 FvH4_5g10890 FvH4_5g10890
malus_domestica MD06G1118700.v1.1 MD14G1126800.v1.1
prunus_persica Prupe.5G129800_v2.0.a1
pyrus_communis pycom06g11020 pycom14g11140
rosa_chinensis RchiOBHm_Chr7g0186971
rosa_laevigata RLG00000004805
rosa_multiflora Rmu_sc0007656.1_g000001
rosa_roxburghii Rroxscaffold_3G00267720
rosa_rugosa Rorug06G0478300
rosa_samantha Rh7AG083900 Rh7BG079000 Rh7CG084900 Rh7DG085800
rosa_wichuraiana Rw7G007380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 797
AasI GACNNNNNNGTC 2 cut(s) 461, 758
Acc36I ACCTGC 2 cut(s) 797, 832
AccB1I GGYRCC 2 cut(s) 293, 377
AccI GTMKAC 1 cut(s) 1101
AccII CGCG 2 cut(s) 146, 303
AclI AACGTT 1 cut(s) 542
AcoI YGGCCR 2 cut(s) 85, 234
AcyI GRCGYC 3 cut(s) 140, 294, 687
AfaI GTAC 2 cut(s) 482, 1190
AfiI CCNNNNNNNGG 5 cut(s) 150, 166, 361, 568, 935
AflIII ACRYGT 1 cut(s) 216
AhdI GACNNNNNGTC 1 cut(s) 1007
AjiI CACGTC 1 cut(s) 754
AjnI CCWGG 2 cut(s) 1018, 1273
AjuI GAANNNNNNNTTGG 2 cut(s) 12, 44
AluBI AGCT 7 cut(s) 289, 661, 742, 831, 913, 1048, 1182
AluI AGCT 7 cut(s) 289, 661, 742, 831, 913, 1048, 1182
Alw21I GWGCWC 6 cut(s) 72, 204, 291, 663, 915, 957
Alw26I GTCTC 3 cut(s) 2, 254, 733
AlwNI CAGNNNCTG 1 cut(s) 525
AoxI GGCC 7 cut(s) 85, 228, 234, 366, 384, 880, 1021
ApeKI GCWGC 4 cut(s) 51, 433, 447, 1221
AspLEI GCGC 2 cut(s) 296, 749
AspS9I GGNCC 7 cut(s) 157, 340, 367, 489, 821, 850, 881
AsuC2I CCSGG 1 cut(s) 734
AsuHPI GGTGA 7 cut(s) 235, 631, 682, 708, 847, 848, 1263
AvaII GGWCC 5 cut(s) 157, 340, 489, 821, 850
BaeGI GKGCMC 2 cut(s) 380, 678
BanI GGYRCC 2 cut(s) 293, 377
BanII GRGCYC 3 cut(s) 291, 663, 915
BauI CACGAG 3 cut(s) 188, 677, 1043
BbsI GAAGAC 1 cut(s) 1035
Bbv12I GWGCWC 6 cut(s) 72, 204, 291, 663, 915, 957
BbvCI CCTCAGC 1 cut(s) 350
BbvI GCAGC 4 cut(s) 63, 420, 459, 1233
BccI CCATC 7 cut(s) 230, 355, 425, 590, 841, 991, 1041
BceAI ACGGC 7 cut(s) 91, 100, 150, 220, 306, 339, 499
BcgI CGANNNNNNTGC 2 cut(s) 109, 143
BciT130I CCWGG 2 cut(s) 1020, 1275
BciVI GTATCC 1 cut(s) 918
BclI TGATCA 1 cut(s) 1062
BcnI CCSGG 1 cut(s) 734
BcoDI GTCTC 3 cut(s) 2, 254, 733
BfaI CTAG 1 cut(s) 471
BfmI CTRYAG 1 cut(s) 316
BfoI RGCGCY 2 cut(s) 297, 750
BfuAI ACCTGC 2 cut(s) 797, 832
BfuI GTATCC 1 cut(s) 918
BlpI GCTNAGC 1 cut(s) 662
Bme1390I CCNGG 3 cut(s) 734, 1020, 1275
Bme18I GGWCC 5 cut(s) 157, 340, 489, 821, 850
BmeRI GACNNNNNGTC 1 cut(s) 1007
BmgBI CACGTC 1 cut(s) 754
BmgT120I GGNCC 7 cut(s) 157, 340, 367, 489, 821, 850, 881
BmiI GGNNCC 9 cut(s) 159, 255, 295, 379, 490, 491, 865, 883, 1328
BmrFI CCNGG 3 cut(s) 734, 1020, 1275
BpiI GAAGAC 1 cut(s) 1035
BplI GAGNNNNNCTC 2 cut(s) 913, 945
BpmI CTGGAG 1 cut(s) 385
Bpu10I CCTNAGC 1 cut(s) 350
Bpu1102I GCTNAGC 1 cut(s) 662
BpuEI CTTGAG 2 cut(s) 392, 1037
BpuMI CCSGG 1 cut(s) 734
BsaHI GRCGYC 3 cut(s) 140, 294, 687
BsaI GGTCTC 2 cut(s) 2, 254
BsaWI WCCGGW 1 cut(s) 655
BsaXI ACNNNNNCTCC 6 cut(s) 383, 413, 1241, 1271, 1307, 1337
Bsc4I CCNNNNNNNGG 5 cut(s) 150, 166, 361, 568, 935
Bse1I ACTGG 1 cut(s) 1330
Bse3DI GCAATG 2 cut(s) 801, 1249
BseBI CCWGG 2 cut(s) 1020, 1275
BseGI GGATG 2 cut(s) 993, 1033
BseLI CCNNNNNNNGG 5 cut(s) 150, 166, 361, 568, 935
BseMI GCAATG 2 cut(s) 801, 1249
BseMII CTCAG 4 cut(s) 364, 676, 909, 1104
BseNI ACTGG 1 cut(s) 1330
BseRI GAGGAG 4 cut(s) 412, 491, 1130, 1262
BseSI GKGCMC 2 cut(s) 380, 678
BseXI GCAGC 4 cut(s) 63, 420, 459, 1233
BseYI CCCAGC 2 cut(s) 1038, 1262
BsgI GTGCAG 1 cut(s) 379
Bsh1236I CGCG 2 cut(s) 146, 303
Bsh1285I CGRYCG 1 cut(s) 301
BshFI GGCC 7 cut(s) 87, 230, 236, 368, 386, 882, 1023
BshNI GGYRCC 2 cut(s) 293, 377
BsiEI CGRYCG 1 cut(s) 301
BsiHKAI GWGCWC 6 cut(s) 72, 204, 291, 663, 915, 957
BsiSI CCGG 4 cut(s) 257, 282, 656, 734
BslFI GGGAC 3 cut(s) 502, 1014, 1123
BslI CCNNNNNNNGG 5 cut(s) 150, 166, 361, 568, 935
BsmAI GTCTC 3 cut(s) 2, 254, 733
BsmFI GGGAC 3 cut(s) 502, 1014, 1123
BsmI GAATGC 3 cut(s) 583, 898, 1359
BsnI GGCC 7 cut(s) 87, 230, 236, 368, 386, 882, 1023
Bso31I GGTCTC 2 cut(s) 2, 254
Bsp1286I GDGCHC 8 cut(s) 72, 204, 291, 380, 663, 678, 915, 957
Bsp143I GATC 1 cut(s) 1062
Bsp1720I GCTNAGC 1 cut(s) 662
Bsp19I CCATGG 1 cut(s) 720
BspANI GGCC 7 cut(s) 87, 230, 236, 368, 386, 882, 1023
BspCNI CTCAG 4 cut(s) 363, 675, 910, 1105
BspFNI CGCG 2 cut(s) 146, 303
BspLI GGNNCC 9 cut(s) 159, 255, 295, 379, 490, 491, 865, 883, 1328
BspMI ACCTGC 2 cut(s) 797, 832
BspT107I GGYRCC 2 cut(s) 293, 377
BspTNI GGTCTC 2 cut(s) 2, 254
BsrDI GCAATG 2 cut(s) 801, 1249
BsrI ACTGG 1 cut(s) 1330
BssMI GATC 1 cut(s) 1062
BssNI GRCGYC 3 cut(s) 140, 294, 687
BssSI CACGAG 3 cut(s) 188, 677, 1043
BssT1I CCWWGG 2 cut(s) 720, 859
Bst2BI CACGAG 3 cut(s) 188, 677, 1043
Bst2UI CCWGG 2 cut(s) 1020, 1275
Bst4CI ACNGT 5 cut(s) 247, 340, 643, 694, 1161
Bst6I CTCTTC 1 cut(s) 53
BstACI GRCGYC 3 cut(s) 140, 294, 687
BstC8I GCNNGC 5 cut(s) 184, 200, 287, 526, 829
BstDEI CTNAG 5 cut(s) 350, 600, 662, 918, 1113
BstDSI CCRYGG 5 cut(s) 132, 144, 336, 642, 720
BstF5I GGATG 2 cut(s) 993, 1033
BstFNI CGCG 2 cut(s) 146, 303
BstH2I RGCGCY 2 cut(s) 297, 750
BstHHI GCGC 2 cut(s) 296, 749
BstKTI GATC 1 cut(s) 1065
BstMAI GTCTC 3 cut(s) 2, 254, 733
BstMBI GATC 1 cut(s) 1062
BstMCI CGRYCG 1 cut(s) 301
BstNI CCWGG 2 cut(s) 1020, 1275
BstNSI RCATGY 2 cut(s) 220, 412
BstSCI CCNGG 3 cut(s) 732, 1018, 1273
BstSFI CTRYAG 1 cut(s) 316
BstSLI GKGCMC 2 cut(s) 380, 678
BstUI CGCG 2 cut(s) 146, 303
BstV1I GCAGC 4 cut(s) 63, 420, 459, 1233
BstV2I GAAGAC 1 cut(s) 1035
BstXI CCANNNNNNTGG 2 cut(s) 194, 589
BsuI GTATCC 1 cut(s) 918
BsuRI GGCC 7 cut(s) 87, 230, 236, 368, 386, 882, 1023
BtgI CCRYGG 5 cut(s) 132, 144, 336, 642, 720
BtrI CACGTC 1 cut(s) 754
BtsCI GGATG 2 cut(s) 993, 1033
BtsI GCAGTG 2 cut(s) 955, 1237
BtsIMutI CAGTG 2 cut(s) 955, 1237
BveI ACCTGC 2 cut(s) 797, 832
Cac8I GCNNGC 5 cut(s) 184, 200, 287, 526, 829
CaiI CAGNNNCTG 1 cut(s) 525
CfoI GCGC 2 cut(s) 296, 749
Cfr13I GGNCC 7 cut(s) 157, 340, 367, 489, 821, 850, 881
Cfr42I CCGCGG 1 cut(s) 147
CseI GACGC 3 cut(s) 129, 676, 1087
Csp6I GTAC 2 cut(s) 481, 1189
CviQI GTAC 2 cut(s) 481, 1189
DdeI CTNAG 5 cut(s) 350, 600, 662, 918, 1113
DinI GGCGCC 1 cut(s) 295
DpnI GATC 1 cut(s) 1064
DpnII GATC 1 cut(s) 1062
DrdI GACNNNNNNGTC 2 cut(s) 461, 758
DriI GACNNNNNGTC 1 cut(s) 1007
DseDI GACNNNNNNGTC 2 cut(s) 461, 758
EaeI YGGCCR 2 cut(s) 85, 234
Eam1104I CTCTTC 1 cut(s) 53
Eam1105I GACNNNNNGTC 1 cut(s) 1007
EarI CTCTTC 1 cut(s) 53
EciI GGCGGA 3 cut(s) 20, 94, 403
Ecl136II GAGCTC 3 cut(s) 289, 661, 913
Eco130I CCWWGG 2 cut(s) 720, 859
Eco147I AGGCCT 2 cut(s) 230, 1023
Eco24I GRGCYC 3 cut(s) 291, 663, 915
Eco31I GGTCTC 2 cut(s) 2, 254
Eco47I GGWCC 5 cut(s) 157, 340, 489, 821, 850
Eco53kI GAGCTC 3 cut(s) 289, 661, 913
EcoICRI GAGCTC 3 cut(s) 289, 661, 913
EcoO109I RGGNCCY 2 cut(s) 157, 489
EcoRII CCWGG 2 cut(s) 1018, 1273
EcoT14I CCWWGG 2 cut(s) 720, 859
EcoT38I GRGCYC 3 cut(s) 291, 663, 915
EgeI GGCGCC 1 cut(s) 295
EheI GGCGCC 1 cut(s) 295
ErhI CCWWGG 2 cut(s) 720, 859
FaqI GGGAC 3 cut(s) 502, 1014, 1123
FauI CCCGC 4 cut(s) 139, 169, 200, 479
FbaI TGATCA 1 cut(s) 1062
FblI GTMKAC 1 cut(s) 1101
FokI GGATG 2 cut(s) 1000, 1020
FriOI GRGCYC 3 cut(s) 291, 663, 915
FspBI CTAG 1 cut(s) 471
GlaI GCGC 2 cut(s) 295, 748
GsaI CCCAGC 2 cut(s) 1042, 1266
GsuI CTGGAG 1 cut(s) 385
HaeII RGCGCY 2 cut(s) 297, 750
HaeIII GGCC 7 cut(s) 87, 230, 236, 368, 386, 882, 1023
HapII CCGG 4 cut(s) 257, 282, 656, 734
HgaI GACGC 3 cut(s) 129, 676, 1087
HhaI GCGC 2 cut(s) 296, 749
Hin1I GRCGYC 3 cut(s) 140, 294, 687
Hin6I GCGC 2 cut(s) 294, 747
HinP1I GCGC 2 cut(s) 294, 747
HincII GTYRAC 3 cut(s) 214, 550, 1102
HindII GTYRAC 3 cut(s) 214, 550, 1102
HindIII AAGCTT 1 cut(s) 829
HinfI GANTC 2 cut(s) 1138, 1361
HpaII CCGG 4 cut(s) 257, 282, 656, 734
HphI GGTGA 7 cut(s) 235, 631, 682, 708, 847, 848, 1263
Hpy166II GTNNAC 8 cut(s) 214, 550, 703, 763, 821, 838, 1088, 1102
Hpy188I TCNGA 2 cut(s) 360, 919
Hpy188III TCNNGA 3 cut(s) 471, 574, 1215
Hpy8I GTNNAC 8 cut(s) 214, 550, 703, 763, 821, 838, 1088, 1102
Hpy99I CGWCG 5 cut(s) 77, 467, 698, 701, 1103
HpyAV CCTTC 6 cut(s) 721, 863, 892, 943, 1246, 1333
HpyCH4III ACNGT 5 cut(s) 247, 340, 643, 694, 1161
HpyCH4IV ACGT 2 cut(s) 542, 753
HpyCH4V TGCA 8 cut(s) 396, 436, 528, 806, 827, 960, 1147, 1357
HpyF3I CTNAG 5 cut(s) 350, 600, 662, 918, 1113
HpySE526I ACGT 2 cut(s) 542, 753
Hsp92I GRCGYC 3 cut(s) 140, 294, 687
HspAI GCGC 2 cut(s) 294, 747
KasI GGCGCC 1 cut(s) 293
KflI GGGWCCC 1 cut(s) 489
Ksp22I TGATCA 1 cut(s) 1062
KspI CCGCGG 1 cut(s) 147
Kzo9I GATC 1 cut(s) 1062
LmnI GCTCC 6 cut(s) 67, 253, 658, 952, 1315, 1326
Lsp1109I GCAGC 4 cut(s) 63, 420, 459, 1233
MaeI CTAG 1 cut(s) 471
MaeII ACGT 2 cut(s) 542, 753
MaeIII GTNAC 2 cut(s) 323, 688
MalI GATC 1 cut(s) 1064
MboI GATC 1 cut(s) 1062
MboII GAAGA 7 cut(s) 23, 70, 301, 505, 1003, 1006, 1040
MfeI CAATTG 1 cut(s) 1293
MhlI GDGCHC 8 cut(s) 72, 204, 291, 380, 663, 678, 915, 957
MluCI AATT 2 cut(s) 669, 1293
Mly113I GGCGCC 1 cut(s) 294
MseI TTAA 3 cut(s) 330, 345, 738
MslI CAYNNNNRTG 4 cut(s) 248, 587, 986, 1175
MspA1I CMGCKG 4 cut(s) 146, 405, 450, 786
MspI CCGG 4 cut(s) 257, 282, 656, 734
MspR9I CCNGG 3 cut(s) 734, 1020, 1275
MunI CAATTG 1 cut(s) 1293
Mva1269I GAATGC 3 cut(s) 583, 898, 1359
MvaI CCWGG 2 cut(s) 1020, 1275
MvnI CGCG 2 cut(s) 146, 303
NarI GGCGCC 1 cut(s) 294
NciI CCSGG 1 cut(s) 734
NcoI CCATGG 1 cut(s) 720
NdeII GATC 1 cut(s) 1062
NlaIV GGNNCC 9 cut(s) 159, 255, 295, 379, 490, 491, 865, 883, 1328
NmeAIII GCCGAG 3 cut(s) 212, 270, 723
NmuCI GTSAC 2 cut(s) 323, 688
NspI RCATGY 2 cut(s) 220, 412
PaqCI CACCTGC 1 cut(s) 797
PceI AGGCCT 2 cut(s) 230, 1023
PciI ACATGT 1 cut(s) 216
PcsI WCGNNNNNNNCGW 3 cut(s) 195, 684, 750
PctI GAATGC 3 cut(s) 583, 898, 1359
PfeI GAWTC 2 cut(s) 1138, 1361
PflFI GACNNNGTC 1 cut(s) 569
PluTI GGCGCC 1 cut(s) 297
PpuMI RGGWCCY 2 cut(s) 157, 489
PscI ACATGT 1 cut(s) 216
Psp124BI GAGCTC 3 cut(s) 291, 663, 915
Psp1406I AACGTT 1 cut(s) 542
Psp5II RGGWCCY 2 cut(s) 157, 489
Psp6I CCWGG 2 cut(s) 1018, 1273
PspFI CCCAGC 2 cut(s) 1038, 1262
PspGI CCWGG 2 cut(s) 1018, 1273
PspN4I GGNNCC 9 cut(s) 159, 255, 295, 379, 490, 491, 865, 883, 1328
PspPI GGNCC 7 cut(s) 157, 340, 367, 489, 821, 850, 881
PspPPI RGGWCCY 2 cut(s) 157, 489
PstNI CAGNNNCTG 1 cut(s) 525
PsyI GACNNNGTC 1 cut(s) 569
RsaI GTAC 2 cut(s) 482, 1190
RsaNI GTAC 2 cut(s) 481, 1189
RseI CAYNNNNRTG 4 cut(s) 248, 587, 986, 1175
SacI GAGCTC 3 cut(s) 291, 663, 915
SacII CCGCGG 1 cut(s) 147
SalI GTCGAC 1 cut(s) 1100
SaqAI TTAA 3 cut(s) 330, 345, 738
Sau3AI GATC 1 cut(s) 1062
Sau96I GGNCC 7 cut(s) 157, 340, 367, 489, 821, 850, 881
ScrFI CCNGG 3 cut(s) 734, 1020, 1275
SduI GDGCHC 8 cut(s) 72, 204, 291, 380, 663, 678, 915, 957
SfcI CTRYAG 1 cut(s) 316
SfoI GGCGCC 1 cut(s) 295
Sfr303I CCGCGG 1 cut(s) 147
SgrBI CCGCGG 1 cut(s) 147
SinI GGWCC 5 cut(s) 157, 340, 489, 821, 850
SmiMI CAYNNNNRTG 4 cut(s) 248, 587, 986, 1175
SmlI CTYRAG 2 cut(s) 371, 1052
SmoI CTYRAG 2 cut(s) 371, 1052
Sse9I AATT 2 cut(s) 669, 1293
SseBI AGGCCT 2 cut(s) 230, 1023
SspDI GGCGCC 1 cut(s) 293
SspMI CTAG 1 cut(s) 471
SstI GAGCTC 3 cut(s) 291, 663, 915
StuI AGGCCT 2 cut(s) 230, 1023
StyD4I CCNGG 3 cut(s) 732, 1018, 1273
StyI CCWWGG 2 cut(s) 720, 859
TaaI ACNGT 5 cut(s) 247, 340, 643, 694, 1161
TaiI ACGT 2 cut(s) 545, 756
TaqI TCGA 7 cut(s) 119, 225, 564, 585, 615, 1026, 1101
TasI AATT 2 cut(s) 669, 1293
TauI GCSGC 8 cut(s) 101, 110, 140, 368, 389, 408, 453, 789
TfiI GAWTC 2 cut(s) 1138, 1361
Tru1I TTAA 3 cut(s) 330, 345, 738
Tru9I TTAA 3 cut(s) 330, 345, 738
TscAI CASTG 2 cut(s) 962, 1237
TseFI GTSAC 2 cut(s) 323, 688
TseI GCWGC 4 cut(s) 51, 433, 447, 1221
Tsp45I GTSAC 2 cut(s) 323, 688
TspDTI ATGAA 8 cut(s) 301, 769, 905, 962, 1004, 1151, 1159, 1209
TspGWI ACGGA 2 cut(s) 80, 415
TspRI CASTG 2 cut(s) 962, 1237
Tth111I GACNNNGTC 1 cut(s) 569
VpaK11BI GGWCC 5 cut(s) 157, 340, 489, 821, 850
XbaI TCTAGA 1 cut(s) 470
XceI RCATGY 2 cut(s) 220, 412
XmiI GTMKAC 1 cut(s) 1101
XspI CTAG 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.