AT1G68630

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0021870
Physical Location & Seq
Forward (+)
25768539 .. 25770260
1722 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G68630.1

Sequence Viewer

Length: 486 bp
ATGTACGGGAATGGGCCGGTGTTTAAGGCGGAGGGGACGTCGTTCCGGGATCAACCATACGCGGAACAGCTTCCTCAGGGACTATGGACCACAGGGCTTTGCGATTGTCACGAAGATGCTCACATTTGTGTTCAGACAGCTATAATGCCATGTGTTTCCTTCGCTCAAAACGTCGAGATCGTAAACAGAGGAACCATACCTTGTATGAACGCAGGTTTGATTCATCTAGCGTTAGGCTTTATTGGTTGTAGCTGGCTCTACGCTTTTCCAAACCGGTCTAGGCTTAGGGAACATTTCGCATTGCCTGAAGAACCATGCCGTGACTTTTTAGTCCATCTCTTTTGCACCCCATGCGCTATCTGCCAAGAGTCCCGAGAACTCAAGAACCGCGGCGCTGATCCTTCTATCGGATGGCTTTCGAATGTAGAGAAATGGAGCCGTGAGAAAGTAACACCTCCCATAGTTGTACCTGGCATGATTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

161

Amino Acids

18.04

Weight (kDa)

6.19

Isoelectric Point (pI)

44.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 29 - 126 6.7e-29 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021870)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68630
malus_domestica MD06G1212800.v1.1
prunus_persica Prupe.5G219300_v2.0.a1
pyrus_communis pycom06g19030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 329
AatII GACGTC 1 cut(s) 41
Acc36I ACCTGC 1 cut(s) 203
AccII CGCG 2 cut(s) 62, 390
AciI CCGC 4 cut(s) 29, 62, 388, 390
AclWI GGATC 2 cut(s) 57, 392
AcuI CTGAAG 1 cut(s) 327
AcyI GRCGYC 1 cut(s) 38
AfaI GTAC 2 cut(s) 5, 468
AfiI CCNNNNNNNGG 1 cut(s) 407
AgeI ACCGGT 1 cut(s) 273
AjnI CCWGG 1 cut(s) 469
AleI CACNNNNGTG 1 cut(s) 126
AluBI AGCT 3 cut(s) 70, 140, 252
AluI AGCT 3 cut(s) 70, 140, 252
AlwI GGATC 2 cut(s) 57, 392
Ama87I CYCGRG 1 cut(s) 372
AoxI GGCC 1 cut(s) 14
AsiGI ACCGGT 1 cut(s) 273
Asp700I GAANNNNTTC 1 cut(s) 69
AspLEI GCGC 2 cut(s) 356, 395
AspS9I GGNCC 2 cut(s) 14, 87
AsuC2I CCSGG 1 cut(s) 47
AsuII TTCGAA 1 cut(s) 419
AvaI CYCGRG 1 cut(s) 372
AvaII GGWCC 1 cut(s) 87
AxyI CCTNAGG 1 cut(s) 75
BccI CCATC 2 cut(s) 342, 405
BceAI ACGGC 2 cut(s) 303, 423
BciT130I CCWGG 1 cut(s) 471
BcnI CCSGG 1 cut(s) 47
BfaI CTAG 2 cut(s) 227, 279
BfoI RGCGCY 1 cut(s) 396
BfuAI ACCTGC 1 cut(s) 203
BisI GCNGC 1 cut(s) 391
BlsI GCNGC 1 cut(s) 392
Bme1390I CCNGG 2 cut(s) 47, 471
Bme18I GGWCC 1 cut(s) 87
BmeT110I CYCGRG 1 cut(s) 372
BmgT120I GGNCC 2 cut(s) 14, 87
BmiI GGNNCC 2 cut(s) 193, 437
BmrFI CCNGG 2 cut(s) 47, 471
BmsI GCATC 1 cut(s) 106
Bpu10I CCTNAGC 1 cut(s) 284
Bpu14I TTCGAA 1 cut(s) 419
BpuEI CTTGAG 1 cut(s) 365
BpuMI CCSGG 1 cut(s) 47
BsaHI GRCGYC 1 cut(s) 38
BsaJI CCNNGG 1 cut(s) 388
BsaWI WCCGGW 1 cut(s) 273
BsaXI ACNNNNNCTCC 2 cut(s) 23, 53
Bsc4I CCNNNNNNNGG 1 cut(s) 407
Bse118I RCCGGY 2 cut(s) 16, 273
Bse21I CCTNAGG 1 cut(s) 75
Bse3DI GCAATG 1 cut(s) 299
BseBI CCWGG 1 cut(s) 471
BseDI CCNNGG 1 cut(s) 388
BseGI GGATG 1 cut(s) 416
BseLI CCNNNNNNNGG 1 cut(s) 407
BseMI GCAATG 1 cut(s) 299
BseMII CTCAG 1 cut(s) 89
Bsh1236I CGCG 2 cut(s) 62, 390
BshFI GGCC 1 cut(s) 16
BshTI ACCGGT 1 cut(s) 273
BsiHKCI CYCGRG 1 cut(s) 372
BsiSI CCGG 3 cut(s) 17, 46, 274
BslFI GGGAC 3 cut(s) 49, 93, 355
BslI CCNNNNNNNGG 1 cut(s) 407
BsmFI GGGAC 3 cut(s) 49, 93, 355
BsnI GGCC 1 cut(s) 16
BsoBI CYCGRG 1 cut(s) 372
Bsp119I TTCGAA 1 cut(s) 419
Bsp143I GATC 3 cut(s) 49, 177, 397
BspACI CCGC 4 cut(s) 29, 62, 388, 390
BspANI GGCC 1 cut(s) 16
BspCNI CTCAG 1 cut(s) 88
BspFNI CGCG 2 cut(s) 62, 390
BspLI GGNNCC 2 cut(s) 193, 437
BspMI ACCTGC 1 cut(s) 203
BspPI GGATC 2 cut(s) 57, 392
BspT104I TTCGAA 1 cut(s) 419
BsrDI GCAATG 1 cut(s) 299
BsrFI RCCGGY 2 cut(s) 16, 273
BssAI RCCGGY 2 cut(s) 16, 273
BssECI CCNNGG 1 cut(s) 388
BssMI GATC 3 cut(s) 49, 177, 397
BssNI GRCGYC 1 cut(s) 38
Bst2UI CCWGG 1 cut(s) 471
BstACI GRCGYC 1 cut(s) 38
BstAPI GCANNNNNTGC 1 cut(s) 351
BstBI TTCGAA 1 cut(s) 419
BstC8I GCNNGC 1 cut(s) 254
BstDEI CTNAG 2 cut(s) 75, 284
BstDSI CCRYGG 1 cut(s) 388
BstF5I GGATG 1 cut(s) 416
BstFNI CGCG 2 cut(s) 62, 390
BstH2I RGCGCY 1 cut(s) 396
BstHHI GCGC 2 cut(s) 356, 395
BstKTI GATC 3 cut(s) 52, 180, 400
BstMBI GATC 3 cut(s) 49, 177, 397
BstMWI GCNNNNNNNGC 2 cut(s) 351, 360
BstNI CCWGG 1 cut(s) 471
BstSCI CCNGG 2 cut(s) 45, 469
BstUI CGCG 2 cut(s) 62, 390
Bsu36I CCTNAGG 1 cut(s) 75
BsuRI GGCC 1 cut(s) 16
BtgI CCRYGG 1 cut(s) 388
BtsCI GGATG 1 cut(s) 416
BveI ACCTGC 1 cut(s) 203
Cac8I GCNNGC 1 cut(s) 254
CfoI GCGC 2 cut(s) 356, 395
Cfr10I RCCGGY 2 cut(s) 16, 273
Cfr13I GGNCC 2 cut(s) 14, 87
Cfr42I CCGCGG 1 cut(s) 391
Csp6I GTAC 2 cut(s) 4, 467
CspAI ACCGGT 1 cut(s) 273
CviAII CATG 4 cut(s) 150, 315, 351, 475
CviQI GTAC 2 cut(s) 4, 467
DdeI CTNAG 2 cut(s) 75, 284
DpnI GATC 3 cut(s) 51, 179, 399
DpnII GATC 3 cut(s) 49, 177, 397
DrdI GACNNNNNNGTC 1 cut(s) 329
DseDI GACNNNNNNGTC 1 cut(s) 329
EciI GGCGGA 1 cut(s) 44
Eco47I GGWCC 1 cut(s) 87
Eco57I CTGAAG 1 cut(s) 327
Eco81I CCTNAGG 1 cut(s) 75
Eco88I CYCGRG 1 cut(s) 372
EcoRII CCWGG 1 cut(s) 469
FaeI CATG 4 cut(s) 153, 318, 354, 478
FaqI GGGAC 3 cut(s) 49, 93, 355
FatI CATG 4 cut(s) 149, 314, 350, 474
Fnu4HI GCNGC 1 cut(s) 391
FokI GGATG 1 cut(s) 423
Fsp4HI GCNGC 1 cut(s) 391
FspBI CTAG 2 cut(s) 227, 279
GlaI GCGC 2 cut(s) 355, 394
GluI GCNGC 1 cut(s) 391
HaeII RGCGCY 1 cut(s) 396
HaeIII GGCC 1 cut(s) 16
HapII CCGG 3 cut(s) 17, 46, 274
HhaI GCGC 2 cut(s) 356, 395
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 4 cut(s) 153, 318, 354, 478
Hin6I GCGC 2 cut(s) 354, 393
HinP1I GCGC 2 cut(s) 354, 393
HinfI GANTC 3 cut(s) 220, 368, 478
HpaII CCGG 3 cut(s) 17, 46, 274
Hpy166II GTNNAC 1 cut(s) 184
Hpy188I TCNGA 2 cut(s) 135, 410
Hpy188III TCNNGA 4 cut(s) 110, 175, 372, 382
Hpy8I GTNNAC 1 cut(s) 184
Hpy99I CGWCG 2 cut(s) 43, 176
HpyAV CCTTC 2 cut(s) 169, 411
HpyCH4IV ACGT 2 cut(s) 38, 171
HpyCH4V TGCA 1 cut(s) 345
HpyF10VI GCNNNNNNNGC 2 cut(s) 351, 360
HpyF3I CTNAG 2 cut(s) 75, 284
HpySE526I ACGT 2 cut(s) 38, 171
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 4 cut(s) 153, 318, 354, 478
HspAI GCGC 2 cut(s) 354, 393
KspI CCGCGG 1 cut(s) 391
Kzo9I GATC 3 cut(s) 49, 177, 397
LmnI GCTCC 1 cut(s) 435
LpnPI CCDG 9 cut(s) 30, 59, 62, 78, 198, 238, 287, 318, 456
LweI GCATC 1 cut(s) 106
MaeI CTAG 2 cut(s) 227, 279
MaeII ACGT 2 cut(s) 38, 171
MaeIII GTNAC 3 cut(s) 107, 320, 448
MalI GATC 3 cut(s) 51, 179, 399
MboI GATC 3 cut(s) 49, 177, 397
MboII GAAGA 2 cut(s) 125, 320
MlyI GAGTC 1 cut(s) 377
MnlI CCTC 4 cut(s) 25, 84, 182, 465
MroXI GAANNNNTTC 1 cut(s) 69
MseI TTAA 1 cut(s) 24
MslI CAYNNNNRTG 2 cut(s) 114, 126
MspA1I CMGCKG 1 cut(s) 390
MspI CCGG 3 cut(s) 17, 46, 274
MspR9I CCNGG 2 cut(s) 47, 471
MvaI CCWGG 1 cut(s) 471
MvnI CGCG 2 cut(s) 62, 390
MwoI GCNNNNNNNGC 2 cut(s) 351, 360
NciI CCSGG 1 cut(s) 47
NdeII GATC 3 cut(s) 49, 177, 397
NlaIII CATG 4 cut(s) 153, 318, 354, 478
NlaIV GGNNCC 2 cut(s) 193, 437
NmuCI GTSAC 2 cut(s) 107, 320
NspV TTCGAA 1 cut(s) 419
OliI CACNNNNGTG 1 cut(s) 126
PcsI WCGNNNNNNNCGW 2 cut(s) 168, 177
PdmI GAANNNNTTC 1 cut(s) 69
PfeI GAWTC 2 cut(s) 220, 478
PfoI TCCNGGA 1 cut(s) 45
PinAI ACCGGT 1 cut(s) 273
PkrI GCNGC 1 cut(s) 392
PleI GAGTC 1 cut(s) 376
PpsI GAGTC 1 cut(s) 376
Psp6I CCWGG 1 cut(s) 469
PspGI CCWGG 1 cut(s) 469
PspN4I GGNNCC 2 cut(s) 193, 437
PspPI GGNCC 2 cut(s) 14, 87
RsaI GTAC 2 cut(s) 5, 468
RsaNI GTAC 2 cut(s) 4, 467
RseI CAYNNNNRTG 2 cut(s) 114, 126
SacII CCGCGG 1 cut(s) 391
SaqAI TTAA 1 cut(s) 24
SatI GCNGC 1 cut(s) 391
Sau3AI GATC 3 cut(s) 49, 177, 397
Sau96I GGNCC 2 cut(s) 14, 87
SchI GAGTC 1 cut(s) 377
ScrFI CCNGG 2 cut(s) 47, 471
SetI ASST 9 cut(s) 41, 72, 142, 174, 202, 217, 254, 457, 472
SfaNI GCATC 1 cut(s) 106
Sfr303I CCGCGG 1 cut(s) 391
SfuI TTCGAA 1 cut(s) 419
SgrBI CCGCGG 1 cut(s) 391
SinI GGWCC 1 cut(s) 87
SmiMI CAYNNNNRTG 2 cut(s) 114, 126
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
SsiI CCGC 4 cut(s) 29, 62, 388, 390
SspMI CTAG 2 cut(s) 227, 279
StyD4I CCNGG 2 cut(s) 45, 469
TaiI ACGT 2 cut(s) 41, 174
TaqI TCGA 3 cut(s) 174, 419, 481
TauI GCSGC 1 cut(s) 393
TfiI GAWTC 2 cut(s) 220, 478
Tru1I TTAA 1 cut(s) 24
Tru9I TTAA 1 cut(s) 24
TseFI GTSAC 2 cut(s) 107, 320
Tsp45I GTSAC 2 cut(s) 107, 320
TspDTI ATGAA 2 cut(s) 212, 221
VpaK11BI GGWCC 1 cut(s) 87
XmnI GAANNNNTTC 1 cut(s) 69
XspI CTAG 2 cut(s) 227, 279
ZraI GACGTC 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.