MD06G1212800.v1.1

PLAC8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0021870
Physical Location & Seq
Reverse (-)
34545223 .. 34546170
948 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1212800.v1.1.491

Sequence Viewer

Length: 525 bp
ATGCATCCTAATTACAACAGTGATCCTAAACAAGCTCCACAAGGAGGAGCATATGGTTATCAGCAGGCGGCGCCTCCACAGGCAGCAACTTACGGCTACCAACAACAGGGGCCGCCACAATGGACAACAGGTCTTTGTGGTTGCTTTGAAGATCCTGGCAGTTGCATGATGACATGCTGCTGTCCCTGCATAACCTTTGGGCAAAATGCTGAGATTATAGATAAAGGAACAACATCGTGCGCTTTCGGGGGTTTAATCTACTTTGCTCTTGCCTATGTGGGGTGTGCTTGTTTGTACTCGTTCTCCTTTAGATCCAAACTGAGAGGTCTCTATGCATTGCCTGAAGATCCATGTGCGGACTGTTGTGTCCATTGCTTGCTTCCTTGTTGTGCTATTTCCCAGGAGTACCGGGAGCTCAAAAACCGCGGATTAGACCCCTCCATAGGTTGGATCGCCAATGCTCAGAAAATGAACCAAGGTGGAAACCCTGCACCTCCATACGTCACACCGGGAATGTACCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

175

Amino Acids

18.82

Weight (kDa)

6.04

Isoelectric Point (pI)

48.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 41 - 138 1.8e-31 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021870)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68630
malus_domestica MD06G1212800.v1.1
prunus_persica Prupe.5G219300_v2.0.a1
pyrus_communis pycom06g19030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 365
AccB1I GGYRCC 1 cut(s) 70
AccB7I CCANNNNNTGG 1 cut(s) 447
AccII CGCG 1 cut(s) 426
AciI CCGC 5 cut(s) 68, 113, 356, 424, 426
AclWI GGATC 5 cut(s) 17, 146, 306, 341, 458
AcuI CTGAAG 1 cut(s) 363
AcyI GRCGYC 1 cut(s) 71
AfaI GTAC 3 cut(s) 296, 407, 518
AfiI CCNNNNNNNGG 5 cut(s) 44, 106, 279, 443, 447
AgsI TTSAA 1 cut(s) 149
AhdI GACNNNNNGTC 1 cut(s) 129
AjnI CCWGG 2 cut(s) 154, 399
AluBI AGCT 2 cut(s) 35, 415
AluI AGCT 2 cut(s) 35, 415
Alw21I GWGCWC 1 cut(s) 417
Alw26I GTCTC 1 cut(s) 332
AlwI GGATC 5 cut(s) 17, 146, 306, 341, 458
AoxI GGCC 1 cut(s) 110
ApeKI GCWGC 2 cut(s) 83, 177
AspLEI GCGC 2 cut(s) 73, 242
AspS9I GGNCC 1 cut(s) 110
AsuC2I CCSGG 2 cut(s) 410, 510
BanI GGYRCC 1 cut(s) 70
BanII GRGCYC 1 cut(s) 417
Bbv12I GWGCWC 1 cut(s) 417
BbvI GCAGC 2 cut(s) 95, 164
BceAI ACGGC 1 cut(s) 109
BciT130I CCWGG 2 cut(s) 156, 401
BcnI CCSGG 2 cut(s) 410, 510
BcoDI GTCTC 1 cut(s) 332
BfoI RGCGCY 1 cut(s) 74
BisI GCNGC 4 cut(s) 69, 84, 113, 178
BlsI GCNGC 4 cut(s) 70, 85, 114, 179
Bme1390I CCNGG 4 cut(s) 156, 401, 410, 510
BmeRI GACNNNNNGTC 1 cut(s) 129
BmgT120I GGNCC 1 cut(s) 110
BmiI GGNNCC 2 cut(s) 72, 111
BmrFI CCNGG 4 cut(s) 156, 401, 410, 510
BmsI GCATC 1 cut(s) 13
BpuMI CCSGG 2 cut(s) 410, 510
BsaHI GRCGYC 1 cut(s) 71
BsaI GGTCTC 1 cut(s) 332
BsaJI CCNNGG 3 cut(s) 399, 424, 475
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bsc4I CCNNNNNNNGG 5 cut(s) 44, 106, 279, 443, 447
Bse3DI GCAATG 2 cut(s) 335, 370
BseBI CCWGG 2 cut(s) 156, 401
BseDI CCNNGG 3 cut(s) 399, 424, 475
BseGI GGATG 1 cut(s) 4
BseLI CCNNNNNNNGG 5 cut(s) 44, 106, 279, 443, 447
BseMI GCAATG 2 cut(s) 335, 370
BseMII CTCAG 3 cut(s) 201, 311, 476
BseRI GAGGAG 1 cut(s) 60
BseXI GCAGC 2 cut(s) 95, 164
BsgI GTGCAG 1 cut(s) 474
Bsh1236I CGCG 1 cut(s) 426
BshFI GGCC 1 cut(s) 112
BshNI GGYRCC 1 cut(s) 70
BsiHKAI GWGCWC 1 cut(s) 417
BsiSI CCGG 2 cut(s) 409, 509
BslFI GGGAC 1 cut(s) 168
BslI CCNNNNNNNGG 5 cut(s) 44, 106, 279, 443, 447
BsmAI GTCTC 1 cut(s) 332
BsmFI GGGAC 1 cut(s) 168
BsnI GGCC 1 cut(s) 112
Bso31I GGTCTC 1 cut(s) 332
Bsp1286I GDGCHC 1 cut(s) 417
Bsp143I GATC 5 cut(s) 22, 151, 311, 346, 450
BspACI CCGC 5 cut(s) 68, 113, 356, 424, 426
BspANI GGCC 1 cut(s) 112
BspCNI CTCAG 3 cut(s) 202, 312, 475
BspFNI CGCG 1 cut(s) 426
BspLI GGNNCC 2 cut(s) 72, 111
BspPI GGATC 5 cut(s) 17, 146, 306, 341, 458
BspT107I GGYRCC 1 cut(s) 70
BspTNI GGTCTC 1 cut(s) 332
BsrDI GCAATG 2 cut(s) 335, 370
BssECI CCNNGG 3 cut(s) 399, 424, 475
BssMI GATC 5 cut(s) 22, 151, 311, 346, 450
BssNI GRCGYC 1 cut(s) 71
BssT1I CCWWGG 1 cut(s) 475
Bst2UI CCWGG 2 cut(s) 156, 401
Bst4CI ACNGT 3 cut(s) 20, 362, 521
BstACI GRCGYC 1 cut(s) 71
BstC8I GCNNGC 2 cut(s) 66, 377
BstDEI CTNAG 3 cut(s) 210, 320, 462
BstDSI CCRYGG 1 cut(s) 424
BstF5I GGATG 1 cut(s) 4
BstFNI CGCG 1 cut(s) 426
BstH2I RGCGCY 1 cut(s) 74
BstHHI GCGC 2 cut(s) 73, 242
BstKTI GATC 5 cut(s) 25, 154, 314, 349, 453
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 5 cut(s) 22, 151, 311, 346, 450
BstMWI GCNNNNNNNGC 2 cut(s) 70, 186
BstNI CCWGG 2 cut(s) 156, 401
BstNSI RCATGY 1 cut(s) 177
BstSCI CCNGG 4 cut(s) 154, 399, 408, 508
BstUI CGCG 1 cut(s) 426
BstV1I GCAGC 2 cut(s) 95, 164
BstX2I RGATCY 3 cut(s) 151, 311, 346
BstYI RGATCY 3 cut(s) 151, 311, 346
BsuRI GGCC 1 cut(s) 112
BtgI CCRYGG 1 cut(s) 424
BtsCI GGATG 1 cut(s) 4
BtsIMutI CAGTG 1 cut(s) 25
Cac8I GCNNGC 2 cut(s) 66, 377
CfoI GCGC 2 cut(s) 73, 242
Cfr13I GGNCC 1 cut(s) 110
Cfr42I CCGCGG 1 cut(s) 427
Csp6I GTAC 3 cut(s) 295, 406, 517
CviAII CATG 3 cut(s) 166, 174, 351
CviJI RGCY 4 cut(s) 35, 96, 112, 415
CviKI_1 RGCY 4 cut(s) 35, 96, 112, 415
CviQI GTAC 3 cut(s) 295, 406, 517
DdeI CTNAG 3 cut(s) 210, 320, 462
DinI GGCGCC 1 cut(s) 72
DpnI GATC 5 cut(s) 24, 153, 313, 348, 452
DpnII GATC 5 cut(s) 22, 151, 311, 346, 450
DrdI GACNNNNNNGTC 1 cut(s) 365
DriI GACNNNNNGTC 1 cut(s) 129
DseDI GACNNNNNNGTC 1 cut(s) 365
Eam1105I GACNNNNNGTC 1 cut(s) 129
Ecl136II GAGCTC 1 cut(s) 415
Eco130I CCWWGG 1 cut(s) 475
Eco24I GRGCYC 1 cut(s) 417
Eco31I GGTCTC 1 cut(s) 332
Eco53kI GAGCTC 1 cut(s) 415
Eco57I CTGAAG 1 cut(s) 363
EcoICRI GAGCTC 1 cut(s) 415
EcoRII CCWGG 2 cut(s) 154, 399
EcoT14I CCWWGG 1 cut(s) 475
EcoT22I ATGCAT 2 cut(s) 6, 337
EcoT38I GRGCYC 1 cut(s) 417
EgeI GGCGCC 1 cut(s) 72
EheI GGCGCC 1 cut(s) 72
ErhI CCWWGG 1 cut(s) 475
FaeI CATG 3 cut(s) 169, 177, 354
FaqI GGGAC 1 cut(s) 168
FatI CATG 3 cut(s) 165, 173, 350
FauNDI CATATG 1 cut(s) 52
Fnu4HI GCNGC 4 cut(s) 69, 84, 113, 178
FriOI GRGCYC 1 cut(s) 417
Fsp4HI GCNGC 4 cut(s) 69, 84, 113, 178
GlaI GCGC 2 cut(s) 72, 241
GluI GCNGC 4 cut(s) 69, 84, 113, 178
HaeII RGCGCY 1 cut(s) 74
HaeIII GGCC 1 cut(s) 112
HapII CCGG 2 cut(s) 409, 509
HhaI GCGC 2 cut(s) 73, 242
Hin1I GRCGYC 1 cut(s) 71
Hin1II CATG 3 cut(s) 169, 177, 354
Hin6I GCGC 2 cut(s) 71, 240
HinP1I GCGC 2 cut(s) 71, 240
HpaII CCGG 2 cut(s) 409, 509
Hpy188I TCNGA 1 cut(s) 465
HpyCH4III ACNGT 3 cut(s) 20, 362, 521
HpyCH4IV ACGT 1 cut(s) 501
HpyCH4V TGCA 5 cut(s) 4, 165, 189, 335, 491
HpyF10VI GCNNNNNNNGC 2 cut(s) 70, 186
HpyF3I CTNAG 3 cut(s) 210, 320, 462
HpySE526I ACGT 1 cut(s) 501
Hsp92I GRCGYC 1 cut(s) 71
Hsp92II CATG 3 cut(s) 169, 177, 354
HspAI GCGC 2 cut(s) 71, 240
KasI GGCGCC 1 cut(s) 70
KspI CCGCGG 1 cut(s) 427
Kzo9I GATC 5 cut(s) 22, 151, 311, 346, 450
LmnI GCTCC 3 cut(s) 40, 47, 412
Lsp1109I GCAGC 2 cut(s) 95, 164
LweI GCATC 1 cut(s) 13
MaeII ACGT 1 cut(s) 501
MaeIII GTNAC 1 cut(s) 502
MalI GATC 5 cut(s) 24, 153, 313, 348, 452
MboI GATC 5 cut(s) 22, 151, 311, 346, 450
MboII GAAGA 2 cut(s) 161, 356
MflI RGATCY 3 cut(s) 151, 311, 346
MhlI GDGCHC 1 cut(s) 417
MluCI AATT 1 cut(s) 10
Mly113I GGCGCC 1 cut(s) 71
MmeI TCCRAC 1 cut(s) 428
MnlI CCTC 5 cut(s) 38, 84, 317, 448, 504
Mph1103I ATGCAT 2 cut(s) 6, 337
MseI TTAA 1 cut(s) 254
MspA1I CMGCKG 1 cut(s) 426
MspI CCGG 2 cut(s) 409, 509
MspR9I CCNGG 4 cut(s) 156, 401, 410, 510
MvaI CCWGG 2 cut(s) 156, 401
MvnI CGCG 1 cut(s) 426
MwoI GCNNNNNNNGC 2 cut(s) 70, 186
NarI GGCGCC 1 cut(s) 71
NciI CCSGG 2 cut(s) 410, 510
NdeI CATATG 1 cut(s) 52
NdeII GATC 5 cut(s) 22, 151, 311, 346, 450
NlaIII CATG 3 cut(s) 169, 177, 354
NlaIV GGNNCC 2 cut(s) 72, 111
NmuCI GTSAC 1 cut(s) 502
NsiI ATGCAT 2 cut(s) 6, 337
NspI RCATGY 1 cut(s) 177
PflMI CCANNNNNTGG 1 cut(s) 447
PkrI GCNGC 4 cut(s) 70, 85, 114, 179
PluTI GGCGCC 1 cut(s) 74
Psp124BI GAGCTC 1 cut(s) 417
Psp6I CCWGG 2 cut(s) 154, 399
PspGI CCWGG 2 cut(s) 154, 399
PspN4I GGNNCC 2 cut(s) 72, 111
PspPI GGNCC 1 cut(s) 110
PsuI RGATCY 3 cut(s) 151, 311, 346
RsaI GTAC 3 cut(s) 296, 407, 518
RsaNI GTAC 3 cut(s) 295, 406, 517
SacI GAGCTC 1 cut(s) 417
SacII CCGCGG 1 cut(s) 427
SaqAI TTAA 1 cut(s) 254
SatI GCNGC 4 cut(s) 69, 84, 113, 178
Sau3AI GATC 5 cut(s) 22, 151, 311, 346, 450
Sau96I GGNCC 1 cut(s) 110
ScrFI CCNGG 4 cut(s) 156, 401, 410, 510
SduI GDGCHC 1 cut(s) 417
SetI ASST 9 cut(s) 37, 133, 197, 328, 417, 448, 481, 496, 504
SfaNI GCATC 1 cut(s) 13
SfoI GGCGCC 1 cut(s) 72
Sfr303I CCGCGG 1 cut(s) 427
SgrBI CCGCGG 1 cut(s) 427
Sse9I AATT 1 cut(s) 10
SsiI CCGC 5 cut(s) 68, 113, 356, 424, 426
SspDI GGCGCC 1 cut(s) 70
SstI GAGCTC 1 cut(s) 417
StyD4I CCNGG 4 cut(s) 154, 399, 408, 508
StyI CCWWGG 1 cut(s) 475
TaaI ACNGT 3 cut(s) 20, 362, 521
TaiI ACGT 1 cut(s) 504
TasI AATT 1 cut(s) 10
TatI WGTACW 1 cut(s) 294
TauI GCSGC 2 cut(s) 71, 115
Tru1I TTAA 1 cut(s) 254
Tru9I TTAA 1 cut(s) 254
TscAI CASTG 1 cut(s) 25
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 2 cut(s) 83, 177
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 1 cut(s) 485
TspRI CASTG 1 cut(s) 25
Van91I CCANNNNNTGG 1 cut(s) 447
XceI RCATGY 1 cut(s) 177
Zsp2I ATGCAT 2 cut(s) 6, 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.