AT1G72970

GMC oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011318
Physical Location & Seq
Forward (+)
27452748 .. 27455964
3217 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G72970.1

Sequence Viewer

Length: 1785 bp
ATGGCTCTCAAGCTCTTTCTCTTTGCTCTTCTTCTCTGTCTCCCGACTTCTCTCTCCTCCACTGCCTCTAAAGGTAAAGAGAAGAAGTCGAAGTTTAATCCATACAGATACACATTCATCGATAAAGCAAGCACATTCTCATCATCTTCCTCATCTTCATTTTCATCCAACGGTCAAGATTCGTCCTACGACTACATAGTCATCGGAGGTGGAACCGCAGGGTGTCCTCTCGCCGCAACGTTGTCGCAGAATTTCAGCGTTCTTGTTTTAGAGAGAGGTGGCGTTCCGTTTACAAACGCAAACGTTTCTTTCCTCAGGAATTTTCACATCGGACTTGCTGACATTTCAGCTTCTTCCGCGTCTCAAGCGTTTGTTTCCACTGACGGCGTTTACAACGCCCGTGCTAGAGTTCTCGGTGGCGGTTCCTGTATTAACGCCGGTTTTTACTCCAGAGCCGATGCTGCGTTCGTGAAGCGAGCAGGATGGGATCCGAAGCTGGTGAAGGAGTCGTATCCATGGGTGGAGAGAGAGATTGTTCATCAGCCAAAGTTAACGTTATGGCAGAAAGCTCTCAGAGACAGTCTTTTAGAGGTTGGAGTCAGACCTTTCAATGGTTTCACTTACGATCACGTTTCCGGAACCAAAATCGGCGGTACAATTTTCGACAGATTCGGCCGTCGTCACACCGCGGCGGAGCTTCTCGCTTACGCTAATCCTCAGAAGCTTAGAGTCTTGATCTACGCCACCGTGCAAAAAATCGTCTTTGACACTTCTGGAACAAGGCCTCGAGTAACAGGAGTAATATTCAAAGATGAGAAAGGTAATCAACACCAGGCTTTACTCTCGAATAGAAAGGGAAGTGAAGTGATCTTATCTAGTGGAGCTATTGGGTCACCACAGATGCTGATGTTAAGTGGGATTGGACCTAAGAAGGAGCTTCAGAGGCTGAAGATTCCTGTGGTTTTAGAGAATGAGCATGTAGGAAAAGGAATGGCTGATAATCCCATGAACACGATCTTGGTGCCTTCAAAGGCGCCTATAGAGCAGTCACTTATTCAGACTGTTGGAATTACAAAGATGGGTGTGTATGTTGAAGCCAGTACTGGCTTTGGGCAATCTCCTGAGAGTATTCATACTCACTATGGGATTATGTCGAACAAGAATGAATTGTTTTCCACCATTCCTGCAAAGCAGAGAAGACCAGAAGCAACGCAAGCTTACATCACAAGAAACAAATACCAACTTCACGAAGCATTCAATGGAAGTTTCATCTTGGAGAAACTTGCTTACCCGATCTCTAGAGGGCATTTGAGCTTGGTCAACACAAATGTTGATGACAACCCTTCAGTCACCTTCAATTACTTTAAACACCCGGTGGATCTCCAACGCTGTGTTGAAGCCATTCGTCTTGTTTCCAAAGTGGTGACGTCTAACCGTTTCTTAAACTACACGCAGTGTGACAAGCAAAACGTACACAAGATGCTTAGCTTAAGCGTCAAGGCAAACATCAATCTAAGGCCAAAGCAACTGAACGATACCAAATCAATGGCTCAGTTCTGCAAAGACACTGTTGTCACAATCTGGCACTACCATGGTGGATGTCTTGTGGGTAAAGTTGTGAGCCCTAACCGCAAAGTTCTTGGTGTCGACAGGCTCAGAGTTATTGATGGTTCAACGTTTGACGAGTCTCCAGGAACCAACCCGCAAGCTACTATGATGATGATGGGAAGATACATGGGAGTCAAGATTCTTCGGGAGAGACTTGGAAACAAAGCTGGTGTTTAG

Protein Analysis

594

Amino Acids

65.34

Weight (kDa)

9.74

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 63 - 335 4.9e-19 GMC oxidoreductase
GMC_oxred_C PF05199 431 - 576 4.9e-26 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011318)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72970 AT1G72970
fragaria_vesca FvH4_6g33360 FvH4_6g33360 FvH4_6g33360
malus_domestica MD09G1193200.v1.1
prunus_persica Prupe.3G049600_v2.0.a1
pyrus_communis pycom09g10980
rosa_chinensis RchiOBHm_Chr2g0143221
rosa_laevigata RLG00000020016
rosa_multiflora Rmu_sc0003950.1_g000006
rosa_roxburghii Rroxscaffold_2G00102010
rosa_rugosa Rorug02G0379500 Rorug02G0379600 Rorug02G0379700
rosa_samantha Rh2AG432400 Rh2BG441000 Rh2CG418200 Rh2DG451100
rosa_wichuraiana Rw2G035300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 197, 1571
AatII GACGTC 1 cut(s) 1430
AccB1I GGYRCC 2 cut(s) 1021, 1033
AccI GTMKAC 1 cut(s) 1647
AccII CGCG 2 cut(s) 359, 689
AccIII TCCGGA 1 cut(s) 635
AclI AACGTT 4 cut(s) 239, 303, 554, 1676
AclWI GGATC 3 cut(s) 482, 495, 1386
AcoI YGGCCR 1 cut(s) 673
AcsI RAATTY 2 cut(s) 250, 319
AcuI CTGAAG 3 cut(s) 923, 968, 1329
AcyI GRCGYC 2 cut(s) 1034, 1427
AdeI CACNNNGTG 2 cut(s) 1375, 1455
AfaI GTAC 3 cut(s) 655, 1102, 1473
AfiI CCNNNNNNNGG 1 cut(s) 611
AflII CTTAAG 1 cut(s) 1489
AgsI TTSAA 8 cut(s) 610, 808, 1029, 1094, 1258, 1357, 1397, 1674
AjnI CCWGG 2 cut(s) 831, 1690
AjuI GAANNNNNNNTTGG 2 cut(s) 1001, 1033
Alw26I GTCTC 5 cut(s) 44, 366, 570, 1692, 1753
AlwI GGATC 3 cut(s) 482, 495, 1386
AlwNI CAGNNNCTG 2 cut(s) 904, 946
Ama87I CYCGRG 1 cut(s) 786
Aor13HI TCCGGA 1 cut(s) 635
AoxI GGCC 3 cut(s) 673, 782, 1517
ApeKI GCWGC 1 cut(s) 461
ApoI RAATTY 2 cut(s) 250, 319
Asp700I GAANNNNTTC 1 cut(s) 1401
AspLEI GCGC 1 cut(s) 1036
AspS9I GGNCC 1 cut(s) 923
AsuC2I CCSGG 1 cut(s) 1373
AsuHPI GGTGA 4 cut(s) 511, 885, 1342, 1435
AvaI CYCGRG 1 cut(s) 786
AvaII GGWCC 1 cut(s) 923
AxyI CCTNAGG 1 cut(s) 314
BamHI GGATCC 1 cut(s) 487
BanI GGYRCC 2 cut(s) 1021, 1033
BanII GRGCYC 1 cut(s) 1625
BbsI GAAGAC 1 cut(s) 1204
BbvI GCAGC 1 cut(s) 448
BccI CCATC 4 cut(s) 477, 1072, 1661, 1717
BceAI ACGGC 2 cut(s) 400, 660
BcgI CGANNNNNNTGC 4 cut(s) 225, 259, 1003, 1037
BciT130I CCWGG 2 cut(s) 833, 1692
BciVI GTATCC 1 cut(s) 522
BcnI CCSGG 1 cut(s) 1373
BcoDI GTCTC 5 cut(s) 44, 366, 570, 1692, 1753
BfaI CTAG 3 cut(s) 405, 876, 1299
BfmI CTRYAG 1 cut(s) 1038
BfoI RGCGCY 1 cut(s) 1037
BfrI CTTAAG 1 cut(s) 1489
BfuI GTATCC 1 cut(s) 522
BisI GCNGC 3 cut(s) 234, 462, 690
BlpI GCTNAGC 1 cut(s) 1484
BlsI GCNGC 3 cut(s) 235, 463, 691
BmcAI AGTACT 1 cut(s) 1102
Bme1390I CCNGG 3 cut(s) 833, 1373, 1692
Bme18I GGWCC 1 cut(s) 923
BmeT110I CYCGRG 1 cut(s) 786
BmgT120I GGNCC 1 cut(s) 923
BmiI GGNNCC 7 cut(s) 214, 424, 489, 640, 1023, 1035, 1696
BmrFI CCNGG 3 cut(s) 833, 1373, 1692
BmsI GCATC 3 cut(s) 448, 891, 1470
BpiI GAAGAC 1 cut(s) 1204
BpmI CTGGAG 2 cut(s) 433, 1674
Bpu1102I GCTNAGC 1 cut(s) 1484
BpuEI CTTGAG 1 cut(s) 348
BpuMI CCSGG 1 cut(s) 1373
Bsa29I ATCGAT 1 cut(s) 120
BsaHI GRCGYC 2 cut(s) 1034, 1427
BsaJI CCNNGG 3 cut(s) 515, 687, 1591
BsaWI WCCGGW 1 cut(s) 635
Bsc4I CCNNNNNNNGG 1 cut(s) 611
Bse118I RCCGGY 1 cut(s) 437
Bse1I ACTGG 2 cut(s) 1098, 1108
Bse21I CCTNAGG 1 cut(s) 314
BseAI TCCGGA 1 cut(s) 635
BseBI CCWGG 2 cut(s) 833, 1692
BseCI ATCGAT 1 cut(s) 120
BseDI CCNNGG 3 cut(s) 515, 687, 1591
BseGI GGATG 3 cut(s) 164, 488, 1604
BseLI CCNNNNNNNGG 1 cut(s) 611
BseMII CTCAG 6 cut(s) 328, 586, 731, 1113, 1565, 1669
BseNI ACTGG 2 cut(s) 1098, 1108
BseRI GAGGAG 1 cut(s) 46
BseX3I CGGCCG 1 cut(s) 673
BseXI GCAGC 1 cut(s) 448
Bsh1236I CGCG 2 cut(s) 359, 689
Bsh1285I CGRYCG 1 cut(s) 676
BshFI GGCC 3 cut(s) 675, 784, 1519
BshNI GGYRCC 2 cut(s) 1021, 1033
BshVI ATCGAT 1 cut(s) 120
BsiEI CGRYCG 1 cut(s) 676
BsiHKCI CYCGRG 1 cut(s) 786
BsiSI CCGG 3 cut(s) 438, 636, 1373
BslI CCNNNNNNNGG 1 cut(s) 611
BsmAI GTCTC 5 cut(s) 44, 366, 570, 1692, 1753
BsmBI CGTCTC 1 cut(s) 366
BsmI GAATGC 1 cut(s) 1253
BsnI GGCC 3 cut(s) 675, 784, 1519
BsoBI CYCGRG 1 cut(s) 786
Bsp1286I GDGCHC 1 cut(s) 1625
Bsp13I TCCGGA 1 cut(s) 635
Bsp143I GATC 7 cut(s) 487, 625, 735, 867, 1014, 1293, 1378
Bsp1720I GCTNAGC 1 cut(s) 1484
Bsp19I CCATGG 2 cut(s) 515, 1591
BspANI GGCC 3 cut(s) 675, 784, 1519
BspCNI CTCAG 6 cut(s) 327, 585, 730, 1114, 1564, 1668
BspDI ATCGAT 1 cut(s) 120
BspEI TCCGGA 1 cut(s) 635
BspFNI CGCG 2 cut(s) 359, 689
BspLI GGNNCC 7 cut(s) 214, 424, 489, 640, 1023, 1035, 1696
BspPI GGATC 3 cut(s) 482, 495, 1386
BspQI GCTCTTC 1 cut(s) 33
BspT107I GGYRCC 2 cut(s) 1021, 1033
BspTI CTTAAG 1 cut(s) 1489
BsrFI RCCGGY 1 cut(s) 437
BsrI ACTGG 2 cut(s) 1098, 1108
BssAI RCCGGY 1 cut(s) 437
BssECI CCNNGG 3 cut(s) 515, 687, 1591
BssMI GATC 7 cut(s) 487, 625, 735, 867, 1014, 1293, 1378
BssNI GRCGYC 2 cut(s) 1034, 1427
BssT1I CCWWGG 2 cut(s) 515, 1591
Bst2UI CCWGG 2 cut(s) 833, 1692
Bst4CI ACNGT 6 cut(s) 173, 581, 748, 1063, 1436, 1570
Bst6I CTCTTC 1 cut(s) 33
BstACI GRCGYC 2 cut(s) 1034, 1427
BstAFI CTTAAG 1 cut(s) 1489
BstC8I GCNNGC 4 cut(s) 130, 477, 1215, 1707
BstDSI CCRYGG 3 cut(s) 515, 687, 1591
BstEII GGTNACC 1 cut(s) 891
BstF5I GGATG 3 cut(s) 164, 488, 1604
BstFNI CGCG 2 cut(s) 359, 689
BstH2I RGCGCY 1 cut(s) 1037
BstHHI GCGC 1 cut(s) 1036
BstKTI GATC 7 cut(s) 490, 628, 738, 870, 1017, 1296, 1381
BstMAI GTCTC 5 cut(s) 44, 366, 570, 1692, 1753
BstMBI GATC 7 cut(s) 487, 625, 735, 867, 1014, 1293, 1378
BstMCI CGRYCG 1 cut(s) 676
BstMWI GCNNNNNNNGC 7 cut(s) 356, 365, 461, 943, 1042, 1214, 1629
BstNI CCWGG 2 cut(s) 833, 1692
BstNSI RCATGY 1 cut(s) 980
BstPI GGTNACC 1 cut(s) 891
BstSCI CCNGG 3 cut(s) 831, 1371, 1690
BstSFI CTRYAG 1 cut(s) 1038
BstUI CGCG 2 cut(s) 359, 689
BstV1I GCAGC 1 cut(s) 448
BstV2I GAAGAC 1 cut(s) 1204
BstX2I RGATCY 2 cut(s) 487, 1378
BstXI CCANNNNNNTGG 1 cut(s) 1546
BstYI RGATCY 2 cut(s) 487, 1378
BstZI CGGCCG 1 cut(s) 673
Bsu15I ATCGAT 1 cut(s) 120
Bsu36I CCTNAGG 1 cut(s) 314
BsuI GTATCC 1 cut(s) 522
BsuRI GGCC 3 cut(s) 675, 784, 1519
BsuTUI ATCGAT 1 cut(s) 120
BtgI CCRYGG 3 cut(s) 515, 687, 1591
BtsCI GGATG 3 cut(s) 164, 488, 1604
BtsI GCAGTG 2 cut(s) 60, 1460
BtsIMutI CAGTG 4 cut(s) 60, 378, 1460, 1566
Cac8I GCNNGC 4 cut(s) 130, 477, 1215, 1707
CaiI CAGNNNCTG 2 cut(s) 904, 946
CfoI GCGC 1 cut(s) 1036
Cfr10I RCCGGY 1 cut(s) 437
Cfr13I GGNCC 1 cut(s) 923
Cfr42I CCGCGG 1 cut(s) 690
ClaI ATCGAT 1 cut(s) 120
CseI GACGC 2 cut(s) 348, 1483
Csp6I GTAC 3 cut(s) 654, 1101, 1472
CviAII CATG 5 cut(s) 516, 977, 1006, 1592, 1735
CviQI GTAC 3 cut(s) 654, 1101, 1472
DinI GGCGCC 1 cut(s) 1035
DpnI GATC 7 cut(s) 489, 627, 737, 869, 1016, 1295, 1380
DpnII GATC 7 cut(s) 487, 625, 735, 867, 1014, 1293, 1378
DraI TTTAAA 1 cut(s) 1366
DraIII CACNNNGTG 2 cut(s) 1375, 1455
DrdI GACNNNNNNGTC 2 cut(s) 197, 1571
DseDI GACNNNNNNGTC 2 cut(s) 197, 1571
EaeI YGGCCR 1 cut(s) 673
EagI CGGCCG 1 cut(s) 673
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
EciI GGCGGA 1 cut(s) 707
EclXI CGGCCG 1 cut(s) 673
Eco130I CCWWGG 2 cut(s) 515, 1591
Eco147I AGGCCT 1 cut(s) 784
Eco24I GRGCYC 1 cut(s) 1625
Eco47I GGWCC 1 cut(s) 923
Eco52I CGGCCG 1 cut(s) 673
Eco57I CTGAAG 3 cut(s) 923, 968, 1329
Eco81I CCTNAGG 1 cut(s) 314
Eco88I CYCGRG 1 cut(s) 786
Eco91I GGTNACC 1 cut(s) 891
EcoO65I GGTNACC 1 cut(s) 891
EcoRII CCWGG 2 cut(s) 831, 1690
EcoT14I CCWWGG 2 cut(s) 515, 1591
EcoT38I GRGCYC 1 cut(s) 1625
EgeI GGCGCC 1 cut(s) 1035
EheI GGCGCC 1 cut(s) 1035
ErhI CCWWGG 2 cut(s) 515, 1591
Esp3I CGTCTC 1 cut(s) 366
FaeI CATG 5 cut(s) 519, 980, 1009, 1595, 1738
FatI CATG 5 cut(s) 515, 976, 1005, 1591, 1734
FauI CCCGC 1 cut(s) 1710
FblI GTMKAC 1 cut(s) 1647
Fnu4HI GCNGC 3 cut(s) 234, 462, 690
FokI GGATG 3 cut(s) 151, 495, 1611
FriOI GRGCYC 1 cut(s) 1625
Fsp4HI GCNGC 3 cut(s) 234, 462, 690
FspBI CTAG 3 cut(s) 405, 876, 1299
GlaI GCGC 1 cut(s) 1035
GluI GCNGC 3 cut(s) 234, 462, 690
GsuI CTGGAG 2 cut(s) 433, 1674
HaeII RGCGCY 1 cut(s) 1037
HaeIII GGCC 3 cut(s) 675, 784, 1519
HapII CCGG 3 cut(s) 438, 636, 1373
HgaI GACGC 2 cut(s) 348, 1483
HhaI GCGC 1 cut(s) 1036
Hin1I GRCGYC 2 cut(s) 1034, 1427
Hin1II CATG 5 cut(s) 519, 980, 1009, 1595, 1738
Hin6I GCGC 1 cut(s) 1034
HinP1I GCGC 1 cut(s) 1034
HincII GTYRAC 3 cut(s) 552, 1321, 1648
HindII GTYRAC 3 cut(s) 552, 1321, 1648
HindIII AAGCTT 2 cut(s) 722, 1215
HinfI GANTC 9 cut(s) 179, 506, 597, 669, 729, 952, 1685, 1740, 1747
HpaI GTTAAC 1 cut(s) 552
HpaII CCGG 3 cut(s) 438, 636, 1373
HphI GGTGA 4 cut(s) 511, 885, 1342, 1435
Hpy166II GTNNAC 6 cut(s) 291, 391, 552, 1321, 1474, 1648
Hpy188I TCNGA 9 cut(s) 206, 332, 492, 575, 602, 720, 942, 1059, 1658
Hpy8I GTNNAC 6 cut(s) 291, 391, 552, 1321, 1474, 1648
Hpy99I CGWCG 1 cut(s) 681
HpyAV CCTTC 5 cut(s) 496, 925, 1035, 1353, 1363
HpyCH4III ACNGT 6 cut(s) 173, 581, 748, 1063, 1436, 1570
HpyCH4IV ACGT 7 cut(s) 239, 303, 554, 630, 1427, 1470, 1676
HpyCH4V TGCA 3 cut(s) 751, 1187, 1560
HpyF10VI GCNNNNNNNGC 7 cut(s) 356, 365, 461, 943, 1042, 1214, 1629
HpySE526I ACGT 7 cut(s) 239, 303, 554, 630, 1427, 1470, 1676
Hsp92I GRCGYC 2 cut(s) 1034, 1427
Hsp92II CATG 5 cut(s) 519, 980, 1009, 1595, 1738
HspAI GCGC 1 cut(s) 1034
KasI GGCGCC 1 cut(s) 1033
Kpn2I TCCGGA 1 cut(s) 635
KspAI GTTAAC 1 cut(s) 552
KspI CCGCGG 1 cut(s) 690
Kzo9I GATC 7 cut(s) 487, 625, 735, 867, 1014, 1293, 1378
LguI GCTCTTC 1 cut(s) 33
LmnI GCTCC 3 cut(s) 694, 881, 934
Lsp1109I GCAGC 1 cut(s) 448
LweI GCATC 3 cut(s) 448, 891, 1470
MaeI CTAG 3 cut(s) 405, 876, 1299
MaeII ACGT 7 cut(s) 239, 303, 554, 630, 1427, 1470, 1676
MaeIII GTNAC 8 cut(s) 680, 790, 891, 1047, 1348, 1423, 1457, 1573
MalI GATC 7 cut(s) 489, 627, 737, 869, 1016, 1295, 1380
MboI GATC 7 cut(s) 487, 625, 735, 867, 1014, 1293, 1378
MflI RGATCY 2 cut(s) 487, 1378
MhlI GDGCHC 1 cut(s) 1625
MluCI AATT 6 cut(s) 250, 319, 657, 1068, 1166, 1357
Mly113I GGCGCC 1 cut(s) 1034
MlyI GAGTC 5 cut(s) 515, 606, 738, 1694, 1749
MmeI TCCRAC 4 cut(s) 192, 574, 1045, 1408
MroI TCCGGA 1 cut(s) 635
MroXI GAANNNNTTC 1 cut(s) 1401
MseI TTAA 7 cut(s) 96, 432, 551, 911, 1365, 1442, 1490
MslI CAYNNNNRTG 1 cut(s) 1590
MspA1I CMGCKG 1 cut(s) 689
MspCI CTTAAG 1 cut(s) 1489
MspI CCGG 3 cut(s) 438, 636, 1373
MspR9I CCNGG 3 cut(s) 833, 1373, 1692
Mva1269I GAATGC 1 cut(s) 1253
MvaI CCWGG 2 cut(s) 833, 1692
MvnI CGCG 2 cut(s) 359, 689
MwoI GCNNNNNNNGC 7 cut(s) 356, 365, 461, 943, 1042, 1214, 1629
NarI GGCGCC 1 cut(s) 1034
NciI CCSGG 1 cut(s) 1373
NcoI CCATGG 2 cut(s) 515, 1591
NdeII GATC 7 cut(s) 487, 625, 735, 867, 1014, 1293, 1378
NlaIII CATG 5 cut(s) 519, 980, 1009, 1595, 1738
NlaIV GGNNCC 7 cut(s) 214, 424, 489, 640, 1023, 1035, 1696
NmuCI GTSAC 7 cut(s) 680, 891, 1047, 1348, 1423, 1457, 1573
NspI RCATGY 1 cut(s) 980
PaeR7I CTCGAG 1 cut(s) 786
PceI AGGCCT 1 cut(s) 784
PciSI GCTCTTC 1 cut(s) 33
PctI GAATGC 1 cut(s) 1253
PdmI GAANNNNTTC 1 cut(s) 1401
PfeI GAWTC 4 cut(s) 179, 669, 952, 1747
PfoI TCCNGGA 1 cut(s) 1690
PkrI GCNGC 3 cut(s) 235, 463, 691
PleI GAGTC 5 cut(s) 514, 605, 737, 1693, 1748
PluTI GGCGCC 1 cut(s) 1037
PpsI GAGTC 5 cut(s) 514, 605, 737, 1693, 1748
Psp1406I AACGTT 4 cut(s) 239, 303, 554, 1676
Psp6I CCWGG 2 cut(s) 831, 1690
PspEI GGTNACC 1 cut(s) 891
PspGI CCWGG 2 cut(s) 831, 1690
PspN4I GGNNCC 7 cut(s) 214, 424, 489, 640, 1023, 1035, 1696
PspPI GGNCC 1 cut(s) 923
PspXI VCTCGAGB 1 cut(s) 786
PstNI CAGNNNCTG 2 cut(s) 904, 946
PsuI RGATCY 2 cut(s) 487, 1378
RsaI GTAC 3 cut(s) 655, 1102, 1473
RsaNI GTAC 3 cut(s) 654, 1101, 1472
RseI CAYNNNNRTG 1 cut(s) 1590
SacII CCGCGG 1 cut(s) 690
SalI GTCGAC 1 cut(s) 1646
SapI GCTCTTC 1 cut(s) 33
SaqAI TTAA 7 cut(s) 96, 432, 551, 911, 1365, 1442, 1490
SatI GCNGC 3 cut(s) 234, 462, 690
Sau3AI GATC 7 cut(s) 487, 625, 735, 867, 1014, 1293, 1378
Sau96I GGNCC 1 cut(s) 923
ScaI AGTACT 1 cut(s) 1102
SchI GAGTC 5 cut(s) 515, 606, 738, 1694, 1749
ScrFI CCNGG 3 cut(s) 833, 1373, 1692
SduI GDGCHC 1 cut(s) 1625
SfaNI GCATC 3 cut(s) 448, 891, 1470
SfcI CTRYAG 1 cut(s) 1038
SfoI GGCGCC 1 cut(s) 1035
Sfr274I CTCGAG 1 cut(s) 786
Sfr303I CCGCGG 1 cut(s) 690
SgrBI CCGCGG 1 cut(s) 690
SinI GGWCC 1 cut(s) 923
SlaI CTCGAG 1 cut(s) 786
SmiMI CAYNNNNRTG 1 cut(s) 1590
SmlI CTYRAG 4 cut(s) 8, 363, 786, 1489
SmoI CTYRAG 4 cut(s) 8, 363, 786, 1489
Sse9I AATT 6 cut(s) 250, 319, 657, 1068, 1166, 1357
SseBI AGGCCT 1 cut(s) 784
SspDI GGCGCC 1 cut(s) 1033
SspI AATATT 1 cut(s) 804
SspMI CTAG 3 cut(s) 405, 876, 1299
StuI AGGCCT 1 cut(s) 784
StyD4I CCNGG 3 cut(s) 831, 1371, 1690
StyI CCWWGG 2 cut(s) 515, 1591
TaaI ACNGT 6 cut(s) 173, 581, 748, 1063, 1436, 1570
TaiI ACGT 7 cut(s) 242, 306, 557, 633, 1430, 1473, 1679
TaqI TCGA 7 cut(s) 89, 120, 663, 787, 845, 1154, 1647
TasI AATT 6 cut(s) 250, 319, 657, 1068, 1166, 1357
TatI WGTACW 1 cut(s) 1100
TauI GCSGC 2 cut(s) 236, 692
TfiI GAWTC 4 cut(s) 179, 669, 952, 1747
Tru1I TTAA 7 cut(s) 96, 432, 551, 911, 1365, 1442, 1490
Tru9I TTAA 7 cut(s) 96, 432, 551, 911, 1365, 1442, 1490
TscAI CASTG 4 cut(s) 67, 385, 1460, 1573
TseFI GTSAC 7 cut(s) 680, 891, 1047, 1348, 1423, 1457, 1573
TseI GCWGC 1 cut(s) 461
Tsp45I GTSAC 7 cut(s) 680, 891, 1047, 1348, 1423, 1457, 1573
TspDTI ATGAA 8 cut(s) 106, 147, 153, 527, 1022, 1121, 1179, 1258
TspGWI ACGGA 1 cut(s) 276
TspRI CASTG 4 cut(s) 67, 385, 1460, 1573
Vha464I CTTAAG 1 cut(s) 1489
VpaK11BI GGWCC 1 cut(s) 923
XapI RAATTY 2 cut(s) 250, 319
XbaI TCTAGA 1 cut(s) 1298
XceI RCATGY 1 cut(s) 980
XhoI CTCGAG 1 cut(s) 786
XmiI GTMKAC 1 cut(s) 1647
XmnI GAANNNNTTC 1 cut(s) 1401
XspI CTAG 3 cut(s) 405, 876, 1299
ZraI GACGTC 1 cut(s) 1428
ZrmI AGTACT 1 cut(s) 1102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.