Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0020255

Physical Location & Seq

Genomic Coordinates Forward (+)

27963926 .. 27965390

Gene Structure 1465 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G74400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1389 bp

ATGAGGTTTATCTTGCGTCATCTGCATTCACTTGGAGTGATCAACAAATTTGATTCCTTTCTTCTACATTTTCACACCAAATCGCTTAAATCTAACCACACTTTGAAGCAATATCTTGAATCTGGGGAACCAATCAAAGCTCTTTTAGATTTCCGGCACCGATTCAGACAAAGTCCTAGTTTTGTTGACAGTTTTTCTGTTTTGTTTGCCATTAAAGTTTCGTCAGCTCAGAAAGCTTCCTCACTTGATGGTAGACAGATCCACGCTCTTGTAAGAAAACTAGGTTTCAATGCCGTTATTCAAATTCAAACATCTTTAGTGGGATTCTACTCTTCCGTGGGTGATGTCGATTATGCACGCCAAGTGTTCGACGAAACGCCTGAGAAACAGAACATTGTCTTGTGGACAGCGATGATTTCAGCTTACACTGAGAATGAGAACTCTGTGGAAGCTATTGAGCTGTTTAAACGAATGGAAGCGGAAAAGATCGAACTTGATGGAGTGATTGTGACTGTGGCTTTATCAGCTTGTGCTGATCTCGGGGCGGTGCAGATGGGAGAAGAGATTTATTCGCGTAGTATCAAGAGAAAACGAAGATTGGCTATGGATTTAACTCTGAGAAATTCACTTCTTAACATGTACGTGAAATCTGGGGAAACTGAGAAAGCTAGGAAACTCTTTGATGAAAGTATGAGAAAAGATGTGACTACTTACACATCTATGATCTTCGGGTATGCTTTAAACGGTCAAGCTCAAGAATCTTTAGAACTCTTCAAGAAAATGAAGACGATTGATCAGAGCCAAGATACTGTCATTACTCCAAATGATGTGACTTTCATCGGCGTCTTGATGGCTTGTAGTCACAGTGGCTTAGTAGAAGAAGGAAAACGACATTTCAAGAGCATGATTATGGATTACAATCTGAAACCTAGAGAAGCTCATTTCGGATGTATGGTGGATTTGTTTTGTCGGTCAGGTCATTTGAAAGATGCTCACGAGTTCATCAATCAGATGCCGATAAAGCCAAACACAGTGATATGGAGAACACTGCTAGGCGCTTGCAGTCTTCACGGGAATGTTGAGCTCGGAGAAGAAGTTCAGAGACGGATCTTTGAGTTAGATCGTGATCATGTCGGAGATTATGTTGCGTTGTCAAATATCTACGCTTCAAAAGGAATGTGGGATGAGAAATCAAAGATGAGGGATCGTGTGAGGAAGAGAAGAATGCCTGGTAAAAGCTGGATTGAACTCGGAAGCATTATCAATGAGTTTGTCTCAGGACCTGATAATAACGATGAGCAGCTGATGATGGGAGAGATTAGTGAGGTTTTGAGATGTTTAGTTTCTTGTATGACAAGTTTTGATTGTGTAATAGGGAAAAAAATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

462

Amino Acids

52.66

Weight (kDa)

8.29

Isoelectric Point (pI)

44.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	131 - 178	8.7e-06	PPR repeat family
PPR	PF01535	133 - 161	5.4e-06	PPR repeat
PPR	PF01535	237 - 263	1.1e-06	PPR repeat
E_motif	PF20431	357 - 416	1.3e-10	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0020255)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74400
malus_domestica	MD06G1197000.v1.1 MD14G1203900.v1.1
prunus_persica	Prupe.5G197200_v2.0.a1
pyrus_communis	pycom06g17380 pycom14g16870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	156
AccI	GTMKAC	1 cut(s)	253
AccII	CGCG	1 cut(s)	574
AciI	CCGC	2 cut(s)	479, 545
AclWI	GGATC	3 cut(s)	253, 1115, 1212
AcsI	RAATTY	3 cut(s)	47, 303, 622
AcyI	GRCGYC	1 cut(s)	843
AfaI	GTAC	1 cut(s)	641
AflIII	ACRYGT	1 cut(s)	636
AjnI	CCWGG	1 cut(s)	1228
AloI	GAACNNNNNNTCC	2 cut(s)	1080, 1112
Alw21I	GWGCWC	1 cut(s)	1086
Alw26I	GTCTC	2 cut(s)	1096, 1279
AlwI	GGATC	3 cut(s)	253, 1115, 1212
AlwNI	CAGNNNCTG	1 cut(s)	1283
Ama87I	CYCGRG	1 cut(s)	539
ApeKI	GCWGC	1 cut(s)	1300
ApoI	RAATTY	3 cut(s)	47, 303, 622
Asp700I	GAANNNNTTC	1 cut(s)	1095
AspLEI	GCGC	1 cut(s)	1058
AspS9I	GGNCC	1 cut(s)	1280
AsuHPI	GGTGA	1 cut(s)	353
AvaI	CYCGRG	1 cut(s)	539
AvaII	GGWCC	1 cut(s)	1280
BanI	GGYRCC	1 cut(s)	156
BanII	GRGCYC	1 cut(s)	1086
BauI	CACGAG	1 cut(s)	995
BbsI	GAAGAC	2 cut(s)	791, 1058
Bbv12I	GWGCWC	1 cut(s)	1086
BbvI	GCAGC	1 cut(s)	1312
BccI	CCATC	5 cut(s)	242, 491, 547, 844, 1303
BceAI	ACGGC	1 cut(s)	278
BciT130I	CCWGG	1 cut(s)	1230
BclI	TGATCA	3 cut(s)	39, 793, 1126
BcoDI	GTCTC	2 cut(s)	1096, 1279
BfaI	CTAG	5 cut(s)	177, 281, 669, 930, 1052
BfoI	RGCGCY	1 cut(s)	1059
BisI	GCNGC	1 cut(s)	1301
BlsI	GCNGC	1 cut(s)	1302
Bme1390I	CCNGG	1 cut(s)	1230
Bme18I	GGWCC	1 cut(s)	1280
BmeT110I	CYCGRG	1 cut(s)	539
BmgT120I	GGNCC	1 cut(s)	1280
BmiI	GGNNCC	2 cut(s)	129, 158
BmrFI	CCNGG	1 cut(s)	1230
BmsI	GCATC	2 cut(s)	979, 1002
BpiI	GAAGAC	2 cut(s)	791, 1058
BplI	GAGNNNNNCTC	2 cut(s)	1259, 1291
BpuEI	CTTGAG	1 cut(s)	738
BsaAI	YACGTR	1 cut(s)	643
BsaBI	GATNNNNATC	2 cut(s)	918, 1125
BsaHI	GRCGYC	1 cut(s)	843
BsaJI	CCNNGG	1 cut(s)	336
BsaXI	ACNNNNNCTCC	6 cut(s)	492, 522, 1080, 1110, 1128, 1158
Bse8I	GATNNNNATC	2 cut(s)	918, 1125
BseBI	CCWGG	1 cut(s)	1230
BseDI	CCNNGG	1 cut(s)	336
BseGI	GGATG	2 cut(s)	953, 1189
BseJI	GATNNNNATC	2 cut(s)	918, 1125
BseMII	CTCAG	6 cut(s)	242, 372, 420, 608, 651, 1290
BseXI	GCAGC	1 cut(s)	1312
BsgI	GTGCAG	1 cut(s)	569
Bsh1236I	CGCG	1 cut(s)	574
BshNI	GGYRCC	1 cut(s)	156
BsiHKAI	GWGCWC	1 cut(s)	1086
BsiHKCI	CYCGRG	1 cut(s)	539
BsiSI	CCGG	1 cut(s)	154
BsmAI	GTCTC	2 cut(s)	1096, 1279
BsmBI	CGTCTC	1 cut(s)	1096
BsmI	GAATGC	2 cut(s)	25, 1230
BsoBI	CYCGRG	1 cut(s)	539
Bsp1286I	GDGCHC	1 cut(s)	1086
BspACI	CCGC	2 cut(s)	479, 545
BspCNI	CTCAG	6 cut(s)	241, 373, 421, 609, 652, 1289
BspFNI	CGCG	1 cut(s)	574
BspLI	GGNNCC	2 cut(s)	129, 158
BspPI	GGATC	3 cut(s)	253, 1115, 1212
BspT107I	GGYRCC	1 cut(s)	156
BssECI	CCNNGG	1 cut(s)	336
BssNI	GRCGYC	1 cut(s)	843
BssSI	CACGAG	1 cut(s)	995
Bst2BI	CACGAG	1 cut(s)	995
Bst2UI	CCWGG	1 cut(s)	1230
Bst4CI	ACNGT	6 cut(s)	191, 514, 746, 811, 866, 1033
Bst6I	CTCTTC	4 cut(s)	337, 555, 776, 1211
BstACI	GRCGYC	1 cut(s)	843
BstBAI	YACGTR	1 cut(s)	643
BstC8I	GCNNGC	2 cut(s)	358, 1060
BstDEI	CTNAG	7 cut(s)	228, 381, 429, 617, 660, 871, 1276
BstDSI	CCRYGG	1 cut(s)	336
BstF5I	GGATG	2 cut(s)	953, 1189
BstFNI	CGCG	1 cut(s)	574
BstH2I	RGCGCY	1 cut(s)	1059
BstHHI	GCGC	1 cut(s)	1058
BstMAI	GTCTC	2 cut(s)	1096, 1279
BstMWI	GCNNNNNNNGC	4 cut(s)	22, 233, 524, 1021
BstNI	CCWGG	1 cut(s)	1230
BstNSI	RCATGY	1 cut(s)	640
BstSCI	CCNGG	1 cut(s)	1228
BstUI	CGCG	1 cut(s)	574
BstV1I	GCAGC	1 cut(s)	1312
BstV2I	GAAGAC	2 cut(s)	791, 1058
BstX2I	RGATCY	2 cut(s)	258, 1107
BstYI	RGATCY	2 cut(s)	258, 1107
BtgI	CCRYGG	1 cut(s)	336
BtgZI	GCGATG	1 cut(s)	425
BtsCI	GGATG	2 cut(s)	953, 1189
BtsI	GCAGTG	1 cut(s)	1046
BtsIMutI	CAGTG	4 cut(s)	426, 871, 1038, 1046
Cac8I	GCNNGC	2 cut(s)	358, 1060
CaiI	CAGNNNCTG	1 cut(s)	1283
CfoI	GCGC	1 cut(s)	1058
Cfr13I	GGNCC	1 cut(s)	1280
CseI	GACGC	2 cut(s)	5, 832
Csp6I	GTAC	1 cut(s)	640
CviAII	CATG	3 cut(s)	637, 904, 1130
CviQI	GTAC	1 cut(s)	640
DdeI	CTNAG	7 cut(s)	228, 381, 429, 617, 660, 871, 1276
DraI	TTTAAA	2 cut(s)	466, 741
Eam1104I	CTCTTC	4 cut(s)	337, 555, 776, 1211
EarI	CTCTTC	4 cut(s)	337, 555, 776, 1211
Ecl136II	GAGCTC	1 cut(s)	1084
Eco24I	GRGCYC	1 cut(s)	1086
Eco47I	GGWCC	1 cut(s)	1280
Eco53kI	GAGCTC	1 cut(s)	1084
Eco88I	CYCGRG	1 cut(s)	539
EcoICRI	GAGCTC	1 cut(s)	1084
EcoO109I	RGGNCCY	1 cut(s)	1280
EcoRII	CCWGG	1 cut(s)	1228
EcoT38I	GRGCYC	1 cut(s)	1086
Esp3I	CGTCTC	1 cut(s)	1096
FaeI	CATG	3 cut(s)	640, 907, 1133
FatI	CATG	3 cut(s)	636, 903, 1129
FbaI	TGATCA	3 cut(s)	39, 793, 1126
FblI	GTMKAC	1 cut(s)	253
Fnu4HI	GCNGC	1 cut(s)	1301
FokI	GGATG	2 cut(s)	960, 1196
FriOI	GRGCYC	1 cut(s)	1086
Fsp4HI	GCNGC	1 cut(s)	1301
FspBI	CTAG	5 cut(s)	177, 281, 669, 930, 1052
GlaI	GCGC	1 cut(s)	1057
GluI	GCNGC	1 cut(s)	1301
HaeII	RGCGCY	1 cut(s)	1059
HapII	CCGG	1 cut(s)	154
HgaI	GACGC	2 cut(s)	5, 832
HhaI	GCGC	1 cut(s)	1058
Hin1I	GRCGYC	1 cut(s)	843
Hin1II	CATG	3 cut(s)	640, 907, 1133
Hin6I	GCGC	1 cut(s)	1056
HinP1I	GCGC	1 cut(s)	1056
HincII	GTYRAC	1 cut(s)	187
HindII	GTYRAC	1 cut(s)	187
HindIII	AAGCTT	1 cut(s)	234
HinfI	GANTC	5 cut(s)	53, 119, 162, 324, 758
HpaII	CCGG	1 cut(s)	154
HphI	GGTGA	1 cut(s)	353
Hpy166II	GTNNAC	3 cut(s)	187, 254, 405
Hpy188III	TCNNGA	9 cut(s)	116, 583, 755, 775, 847, 898, 995, 1124, 1278
Hpy8I	GTNNAC	3 cut(s)	187, 254, 405
Hpy99I	CGWCG	1 cut(s)	374
HpyAV	CCTTC	1 cut(s)	875
HpyCH4III	ACNGT	6 cut(s)	191, 514, 746, 811, 866, 1033
HpyCH4IV	ACGT	1 cut(s)	642
HpyCH4V	TGCA	4 cut(s)	25, 356, 550, 1062
HpyF10VI	GCNNNNNNNGC	4 cut(s)	22, 233, 524, 1021
HpyF3I	CTNAG	7 cut(s)	228, 381, 429, 617, 660, 871, 1276
HpySE526I	ACGT	1 cut(s)	642
Hsp92I	GRCGYC	1 cut(s)	843
Hsp92II	CATG	3 cut(s)	640, 907, 1133
HspAI	GCGC	1 cut(s)	1056
Ksp22I	TGATCA	3 cut(s)	39, 793, 1126
Lsp1109I	GCAGC	1 cut(s)	1312
LweI	GCATC	2 cut(s)	979, 1002
MaeI	CTAG	5 cut(s)	177, 281, 669, 930, 1052
MaeII	ACGT	1 cut(s)	642
MaeIII	GTNAC	4 cut(s)	508, 703, 829, 860
MflI	RGATCY	2 cut(s)	258, 1107
MhlI	GDGCHC	1 cut(s)	1086
MluCI	AATT	3 cut(s)	47, 303, 622
MmeI	TCCRAC	1 cut(s)	1114
MnlI	CCTC	4 cut(s)	250, 1194, 1206, 1318
MroXI	GAANNNNTTC	1 cut(s)	1095
MseI	TTAA	6 cut(s)	87, 213, 465, 611, 633, 740
MslI	CAYNNNNRTG	4 cut(s)	641, 719, 908, 1074
MspA1I	CMGCKG	1 cut(s)	1303
MspI	CCGG	1 cut(s)	154
MspR9I	CCNGG	1 cut(s)	1230
MssI	GTTTAAAC	1 cut(s)	466
Mva1269I	GAATGC	2 cut(s)	25, 1230
MvaI	CCWGG	1 cut(s)	1230
MvnI	CGCG	1 cut(s)	574
MwoI	GCNNNNNNNGC	4 cut(s)	22, 233, 524, 1021
NlaIII	CATG	3 cut(s)	640, 907, 1133
NlaIV	GGNNCC	2 cut(s)	129, 158
NmuCI	GTSAC	4 cut(s)	508, 703, 829, 860
NspI	RCATGY	1 cut(s)	640
PciI	ACATGT	1 cut(s)	636
PctI	GAATGC	2 cut(s)	25, 1230
PdmI	GAANNNNTTC	1 cut(s)	1095
PfeI	GAWTC	5 cut(s)	53, 119, 162, 324, 758
PflFI	GACNNNGTC	1 cut(s)	171
PkrI	GCNGC	1 cut(s)	1302
PmeI	GTTTAAAC	1 cut(s)	466
Ppu21I	YACGTR	1 cut(s)	643
PpuMI	RGGWCCY	1 cut(s)	1280
PscI	ACATGT	1 cut(s)	636
Psp124BI	GAGCTC	1 cut(s)	1086
Psp5II	RGGWCCY	1 cut(s)	1280
Psp6I	CCWGG	1 cut(s)	1228
PspGI	CCWGG	1 cut(s)	1228
PspN4I	GGNNCC	2 cut(s)	129, 158
PspPI	GGNCC	1 cut(s)	1280
PspPPI	RGGWCCY	1 cut(s)	1280
PstNI	CAGNNNCTG	1 cut(s)	1283
PsuI	RGATCY	2 cut(s)	258, 1107
PsyI	GACNNNGTC	1 cut(s)	171
PvuII	CAGCTG	1 cut(s)	1303
RsaI	GTAC	1 cut(s)	641
RsaNI	GTAC	1 cut(s)	640
RseI	CAYNNNNRTG	4 cut(s)	641, 719, 908, 1074
SacI	GAGCTC	1 cut(s)	1086
SaqAI	TTAA	6 cut(s)	87, 213, 465, 611, 633, 740
SatI	GCNGC	1 cut(s)	1301
Sau96I	GGNCC	1 cut(s)	1280
ScrFI	CCNGG	1 cut(s)	1230
SduI	GDGCHC	1 cut(s)	1086
SfaNI	GCATC	2 cut(s)	979, 1002
SinI	GGWCC	1 cut(s)	1280
SmiMI	CAYNNNNRTG	4 cut(s)	641, 719, 908, 1074
SmlI	CTYRAG	1 cut(s)	753
SmoI	CTYRAG	1 cut(s)	753
Sse9I	AATT	3 cut(s)	47, 303, 622
SsiI	CCGC	2 cut(s)	479, 545
SspMI	CTAG	5 cut(s)	177, 281, 669, 930, 1052
SstI	GAGCTC	1 cut(s)	1086
StyD4I	CCNGG	1 cut(s)	1228
TaaI	ACNGT	6 cut(s)	191, 514, 746, 811, 866, 1033
TaiI	ACGT	1 cut(s)	645
TaqI	TCGA	3 cut(s)	348, 369, 489
TaqII	GACCGA	1 cut(s)	960
TasI	AATT	3 cut(s)	47, 303, 622
TfiI	GAWTC	5 cut(s)	53, 119, 162, 324, 758
Tru1I	TTAA	6 cut(s)	87, 213, 465, 611, 633, 740
Tru9I	TTAA	6 cut(s)	87, 213, 465, 611, 633, 740
TscAI	CASTG	4 cut(s)	433, 871, 1038, 1053
TseFI	GTSAC	4 cut(s)	508, 703, 829, 860
TseI	GCWGC	1 cut(s)	1300
Tsp45I	GTSAC	4 cut(s)	508, 703, 829, 860
TspDTI	ATGAA	4 cut(s)	699, 797, 826, 991
TspGWI	ACGGA	2 cut(s)	325, 1120
TspRI	CASTG	4 cut(s)	433, 871, 1038, 1053
Tth111I	GACNNNGTC	1 cut(s)	171
VpaK11BI	GGWCC	1 cut(s)	1280
XapI	RAATTY	3 cut(s)	47, 303, 622
XceI	RCATGY	1 cut(s)	640
XmiI	GTMKAC	1 cut(s)	253
XmnI	GAANNNNTTC	1 cut(s)	1095
XspI	CTAG	5 cut(s)	177, 281, 669, 930, 1052

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.