AT2G07727

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0018296
Physical Location & Seq
Forward (+)
3449534 .. 3452776
3243 bp
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UTR
Exon/CDS
Intron
AT2G07727.1

Sequence Viewer

Length: 1182 bp
ATGACTATAAGGAACCAACGATTCTCTCTTCTTAAACAACCTATATCCTCCACACTTAATCAGCATTTAGTAGATTATCCAACCCCGAGCAATCTTAGTTATTGGTGGGGGTTCGGTCCGTTAGCTGGTATTTGTTTAGTCATTCAGATAGTGACTGGCGTTTTTTTAGCTATGCATTACACACCTCATGTGGATTTAGCTTTCAACAGCGTAGAACACATTATGAGAGATGTTGAAGGGGGCTGGTTGCTCCGTTATATGCATGCTAATGGGGCAAGTATGTTTCTTATTGTGGTTTACCTTCATATTTTTCGTGGTCTATATCATGCGAGTTATAGCAGTCCTAGGGAATTTGTTTGGTGTCTTGGAGTTGTAATCTTCCTATTAATGATTGTGACAGCTTTTATAGGATATGTACTACCTTGGGGTCAGATGAGCTTTTGGGGAGCTACAGTAATTACAAGCTTAGCTAGCGCCATACCTGTAGTAGGAGATACCATAGTGACTTGGCTTTGGGGTGGTTTCTCCGTGGACAATGCCACCTTAAATCGTTTTTTTAGTCTTCATCATTTACTCCCCTTTATTTTAGTAGGCGCCAGTCTTCTTCATCTGGCCGCATTGCATCAATATGGATCAAATAATCCATTGGGTGTACATTCTGAGATGGATAAAATAGCTTTTTACCCTTATTTTTATGTCAAGGATCTAGTTGGTTGGGTAGCTTTTGCTATCTTTTTTTCTATTTGGATTTTTTATGCTCCTAATGTTTTGGGACATCCCGACAATTATATACCTGCTAATCCGATGTCCACCCCGCCTCATATTGTGCCGGAATGGTATTTCCTACCGATCCATGCCATTCTTCGTAGTATACCTGACAAAGCGGGAGGTGTAGCCGCAATAGCACCAGTTTTTATATGTCTCTTGGCTTTACCTTTTTTTAAAAGTATGTATGTGCGTAGTTCAAGTTTTCGACCGATTCACCAAGGAATGTTTTGGTTGCTTTTGGCGGATTGCTTACTACTAGGTTGGATCGGATGTCAACCTGTGGAGGCACCATTTGTTACTATTGGACAAATTTCTCCTTTGGTTTTCTTCTTGTTCTTTGCCATAACGCCCATTCTGGGACGAGTTGGAAGAGGAATTCCTAATTCTTACACGGATGAGACTGATCACACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko02020 ko04260 ko04714 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map02020 map04260 map04714 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
Cytochrom_B_C Cytochrome_B

Protein Analysis

393

Amino Acids

44.15

Weight (kDa)

6.77

Isoelectric Point (pI)

37.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrome_B PF00033 24 - 211 4.9e-84 Cytochrome b/b6/petB
Cytochrom_B_N_2 PF13631 94 - 260 9.1e-45 Cytochrome b(N-terminal)/b6/petB
Cytochrom_B_C PF00032 265 - 366 6.8e-35 Cytochrome b(C-terminal)/b6/petD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018296)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07727 ATMG00220
rosa_chinensis RchiOBHm_MTg0499071
rosa_laevigata RLG00000000274 RLG00000000408 RLG00000000426 RLG00000000483
rosa_roxburghii Rroxscaffold_2G00119480
rosa_samantha Rh1BG203000 Rh1DG230000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 802
AccB1I GGYRCC 2 cut(s) 593, 1054
AccI GTMKAC 1 cut(s) 871
AciI CCGC 5 cut(s) 615, 815, 884, 897, 1010
AclWI GGATC 4 cut(s) 640, 711, 844, 1040
AcoI YGGCCR 1 cut(s) 612
AcsI RAATTY 3 cut(s) 350, 1077, 1143
AcyI GRCGYC 1 cut(s) 594
AfaI GTAC 2 cut(s) 417, 654
AfiI CCNNNNNNNGG 3 cut(s) 125, 488, 1124
AgsI TTSAA 3 cut(s) 205, 236, 966
Alw26I GTCTC 2 cut(s) 926, 1160
AlwI GGATC 4 cut(s) 640, 711, 844, 1040
Ama87I CYCGRG 1 cut(s) 85
AoxI GGCC 1 cut(s) 612
ApoI RAATTY 3 cut(s) 350, 1077, 1143
AseI ATTAAT 1 cut(s) 386
AspA2I CCTAGG 1 cut(s) 344
AspLEI GCGC 2 cut(s) 476, 596
AspS9I GGNCC 1 cut(s) 116
AsuHPI GGTGA 1 cut(s) 974
AsuNHI GCTAGC 1 cut(s) 470
AvaI CYCGRG 1 cut(s) 85
AvaII GGWCC 1 cut(s) 116
AvrII CCTAGG 1 cut(s) 344
BaeI ACNNNNGTAYC 2 cut(s) 486, 519
BanI GGYRCC 2 cut(s) 593, 1054
BbsI GAAGAC 2 cut(s) 554, 593
BccI CCATC 1 cut(s) 658
BclI TGATCA 1 cut(s) 1171
BcoDI GTCTC 2 cut(s) 926, 1160
BfaI CTAG 4 cut(s) 345, 471, 707, 1025
BfmI CTRYAG 2 cut(s) 450, 483
BfoI RGCGCY 2 cut(s) 477, 597
BfuAI ACCTGC 1 cut(s) 802
BisI GCNGC 2 cut(s) 615, 897
BlnI CCTAGG 1 cut(s) 344
BlpI GCTNAGC 1 cut(s) 466
BlsI GCNGC 2 cut(s) 616, 898
Bme18I GGWCC 1 cut(s) 116
BmeT110I CYCGRG 1 cut(s) 85
BmgT120I GGNCC 1 cut(s) 116
BmiI GGNNCC 3 cut(s) 14, 595, 1056
BmsI GCATC 1 cut(s) 631
BmtI GCTAGC 1 cut(s) 474
BpiI GAAGAC 2 cut(s) 554, 593
Bpu1102I GCTNAGC 1 cut(s) 466
BsaHI GRCGYC 1 cut(s) 594
BsaJI CCNNGG 4 cut(s) 344, 422, 528, 985
BsaXI ACNNNNNCTCC 2 cut(s) 438, 468
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 488, 1124
Bse1I ACTGG 3 cut(s) 160, 597, 908
Bse3DI GCAATG 1 cut(s) 617
BseDI CCNNGG 4 cut(s) 344, 422, 528, 985
BseGI GGATG 3 cut(s) 775, 1043, 1168
BseLI CCNNNNNNNGG 3 cut(s) 125, 488, 1124
BseMI GCAATG 1 cut(s) 617
BseMII CTCAG 1 cut(s) 651
BseNI ACTGG 3 cut(s) 160, 597, 908
Bsh1285I CGRYCG 1 cut(s) 977
BshFI GGCC 1 cut(s) 614
BshNI GGYRCC 2 cut(s) 593, 1054
BsiEI CGRYCG 1 cut(s) 977
BsiHKCI CYCGRG 1 cut(s) 85
BsiSI CCGG 1 cut(s) 830
BslFI GGGAC 2 cut(s) 786, 1140
BslI CCNNNNNNNGG 3 cut(s) 125, 488, 1124
BsmAI GTCTC 2 cut(s) 926, 1160
BsmFI GGGAC 2 cut(s) 786, 1140
BsnI GGCC 1 cut(s) 614
BsoBI CYCGRG 1 cut(s) 85
Bsp1407I TGTACA 1 cut(s) 652
Bsp143I GATC 5 cut(s) 632, 703, 849, 1032, 1171
Bsp1720I GCTNAGC 1 cut(s) 466
BspACI CCGC 5 cut(s) 615, 815, 884, 897, 1010
BspANI GGCC 1 cut(s) 614
BspCNI CTCAG 1 cut(s) 652
BspLI GGNNCC 3 cut(s) 14, 595, 1056
BspMI ACCTGC 1 cut(s) 802
BspOI GCTAGC 1 cut(s) 474
BspPI GGATC 4 cut(s) 640, 711, 844, 1040
BspT107I GGYRCC 2 cut(s) 593, 1054
BsrDI GCAATG 1 cut(s) 617
BsrGI TGTACA 1 cut(s) 652
BsrI ACTGG 3 cut(s) 160, 597, 908
BssECI CCNNGG 4 cut(s) 344, 422, 528, 985
BssMI GATC 5 cut(s) 632, 703, 849, 1032, 1171
BssNAI GTATAC 1 cut(s) 872
BssNI GRCGYC 1 cut(s) 594
BssT1I CCWWGG 3 cut(s) 344, 422, 985
Bst1107I GTATAC 1 cut(s) 872
Bst4CI ACNGT 1 cut(s) 454
Bst6I CTCTTC 2 cut(s) 33, 1132
BstACI GRCGYC 1 cut(s) 594
BstAUI TGTACA 1 cut(s) 652
BstC8I GCNNGC 2 cut(s) 264, 472
BstDEI CTNAG 3 cut(s) 95, 466, 660
BstDSI CCRYGG 1 cut(s) 528
BstENI CCTNNNNNAGG 1 cut(s) 486
BstF5I GGATG 3 cut(s) 775, 1043, 1168
BstH2I RGCGCY 2 cut(s) 477, 597
BstHHI GCGC 2 cut(s) 476, 596
BstKTI GATC 5 cut(s) 635, 706, 852, 1035, 1174
BstMAI GTCTC 2 cut(s) 926, 1160
BstMBI GATC 5 cut(s) 632, 703, 849, 1032, 1171
BstMCI CGRYCG 1 cut(s) 977
BstMWI GCNNNNNNNGC 3 cut(s) 272, 471, 902
BstNSI RCATGY 1 cut(s) 266
BstSFI CTRYAG 2 cut(s) 450, 483
BstV2I GAAGAC 2 cut(s) 554, 593
BstX2I RGATCY 1 cut(s) 703
BstYI RGATCY 1 cut(s) 703
BstZ17I GTATAC 1 cut(s) 872
BsuRI GGCC 1 cut(s) 614
BtgI CCRYGG 1 cut(s) 528
BtsCI GGATG 3 cut(s) 775, 1043, 1168
BveI ACCTGC 1 cut(s) 802
Cac8I GCNNGC 2 cut(s) 264, 472
CfoI GCGC 2 cut(s) 476, 596
Cfr13I GGNCC 1 cut(s) 116
CpoI CGGWCCG 1 cut(s) 116
Csp6I GTAC 2 cut(s) 416, 653
CspI CGGWCCG 1 cut(s) 116
CviAII CATG 4 cut(s) 188, 263, 326, 854
CviQI GTAC 2 cut(s) 416, 653
DdeI CTNAG 3 cut(s) 95, 466, 660
DinI GGCGCC 1 cut(s) 595
DpnI GATC 5 cut(s) 634, 705, 851, 1034, 1173
DpnII GATC 5 cut(s) 632, 703, 849, 1032, 1171
DraI TTTAAA 1 cut(s) 943
EaeI YGGCCR 1 cut(s) 612
Eam1104I CTCTTC 2 cut(s) 33, 1132
EarI CTCTTC 2 cut(s) 33, 1132
EciI GGCGGA 1 cut(s) 1025
Eco130I CCWWGG 3 cut(s) 344, 422, 985
Eco47I GGWCC 1 cut(s) 116
Eco88I CYCGRG 1 cut(s) 85
EcoNI CCTNNNNNAGG 1 cut(s) 486
EcoRI GAATTC 1 cut(s) 1143
EcoT14I CCWWGG 3 cut(s) 344, 422, 985
EcoT22I ATGCAT 2 cut(s) 177, 264
EgeI GGCGCC 1 cut(s) 595
EheI GGCGCC 1 cut(s) 595
ErhI CCWWGG 3 cut(s) 344, 422, 985
FaeI CATG 4 cut(s) 191, 266, 329, 857
FaqI GGGAC 2 cut(s) 786, 1140
FatI CATG 4 cut(s) 187, 262, 325, 853
FauI CCCGC 2 cut(s) 822, 877
FbaI TGATCA 1 cut(s) 1171
FblI GTMKAC 1 cut(s) 871
Fnu4HI GCNGC 2 cut(s) 615, 897
FokI GGATG 3 cut(s) 762, 1050, 1175
Fsp4HI GCNGC 2 cut(s) 615, 897
FspBI CTAG 4 cut(s) 345, 471, 707, 1025
GlaI GCGC 2 cut(s) 475, 595
GluI GCNGC 2 cut(s) 615, 897
HaeII RGCGCY 2 cut(s) 477, 597
HaeIII GGCC 1 cut(s) 614
HapII CCGG 1 cut(s) 830
HhaI GCGC 2 cut(s) 476, 596
Hin1I GRCGYC 1 cut(s) 594
Hin1II CATG 4 cut(s) 191, 266, 329, 857
Hin6I GCGC 2 cut(s) 474, 594
HinP1I GCGC 2 cut(s) 474, 594
HincII GTYRAC 1 cut(s) 1043
HindII GTYRAC 1 cut(s) 1043
HindIII AAGCTT 1 cut(s) 463
HinfI GANTC 2 cut(s) 21, 979
HpaII CCGG 1 cut(s) 830
HphI GGTGA 1 cut(s) 974
Hpy166II GTNNAC 6 cut(s) 298, 532, 653, 810, 872, 1043
Hpy188I TCNGA 5 cut(s) 147, 432, 661, 804, 1037
Hpy188III TCNNGA 1 cut(s) 779
Hpy8I GTNNAC 6 cut(s) 298, 532, 653, 810, 872, 1043
HpyAV CCTTC 2 cut(s) 230, 311
HpyCH4III ACNGT 1 cut(s) 454
HpyCH4V TGCA 3 cut(s) 175, 262, 622
HpyF10VI GCNNNNNNNGC 3 cut(s) 272, 471, 902
HpyF3I CTNAG 3 cut(s) 95, 466, 660
Hsp92I GRCGYC 1 cut(s) 594
Hsp92II CATG 4 cut(s) 191, 266, 329, 857
HspAI GCGC 2 cut(s) 474, 594
KasI GGCGCC 1 cut(s) 593
Ksp22I TGATCA 1 cut(s) 1171
Kzo9I GATC 5 cut(s) 632, 703, 849, 1032, 1171
LmnI GCTCC 3 cut(s) 255, 446, 763
LweI GCATC 1 cut(s) 631
MaeI CTAG 4 cut(s) 345, 471, 707, 1025
MaeIII GTNAC 4 cut(s) 151, 394, 502, 1063
MalI GATC 5 cut(s) 634, 705, 851, 1034, 1173
MboI GATC 5 cut(s) 632, 703, 849, 1032, 1171
MboII GAAGA 8 cut(s) 20, 370, 554, 593, 596, 854, 1087, 1149
MflI RGATCY 1 cut(s) 703
MluCI AATT 6 cut(s) 350, 456, 784, 1077, 1143, 1150
Mly113I GGCGCC 1 cut(s) 594
MmeI TCCRAC 3 cut(s) 104, 1010, 1114
MnlI CCTC 6 cut(s) 58, 195, 828, 881, 1045, 1133
Mph1103I ATGCAT 2 cut(s) 177, 264
MseI TTAA 5 cut(s) 33, 57, 386, 545, 942
MslI CAYNNNNRTG 2 cut(s) 267, 627
MspI CCGG 1 cut(s) 830
MwoI GCNNNNNNNGC 3 cut(s) 272, 471, 902
NarI GGCGCC 1 cut(s) 594
NdeII GATC 5 cut(s) 632, 703, 849, 1032, 1171
NheI GCTAGC 1 cut(s) 470
NlaIII CATG 4 cut(s) 191, 266, 329, 857
NlaIV GGNNCC 3 cut(s) 14, 595, 1056
NmuCI GTSAC 3 cut(s) 151, 394, 502
NsiI ATGCAT 2 cut(s) 177, 264
NspI RCATGY 1 cut(s) 266
PaeI GCATGC 1 cut(s) 266
PfeI GAWTC 2 cut(s) 21, 979
PkrI GCNGC 2 cut(s) 616, 898
PluTI GGCGCC 1 cut(s) 597
PshBI ATTAAT 1 cut(s) 386
PspN4I GGNNCC 3 cut(s) 14, 595, 1056
PspPI GGNCC 1 cut(s) 116
PsuI RGATCY 1 cut(s) 703
RsaI GTAC 2 cut(s) 417, 654
RsaNI GTAC 2 cut(s) 416, 653
RseI CAYNNNNRTG 2 cut(s) 267, 627
Rsr2I CGGWCCG 1 cut(s) 116
RsrII CGGWCCG 1 cut(s) 116
SaqAI TTAA 5 cut(s) 33, 57, 386, 545, 942
SatI GCNGC 2 cut(s) 615, 897
Sau3AI GATC 5 cut(s) 632, 703, 849, 1032, 1171
Sau96I GGNCC 1 cut(s) 116
SfaNI GCATC 1 cut(s) 631
SfcI CTRYAG 2 cut(s) 450, 483
SfoI GGCGCC 1 cut(s) 595
SinI GGWCC 1 cut(s) 116
SmiMI CAYNNNNRTG 2 cut(s) 267, 627
SphI GCATGC 1 cut(s) 266
Sse9I AATT 6 cut(s) 350, 456, 784, 1077, 1143, 1150
SsiI CCGC 5 cut(s) 615, 815, 884, 897, 1010
SspDI GGCGCC 1 cut(s) 593
SspMI CTAG 4 cut(s) 345, 471, 707, 1025
StyI CCWWGG 3 cut(s) 344, 422, 985
TaaI ACNGT 1 cut(s) 454
TaqI TCGA 1 cut(s) 973
TaqII GACCGA 2 cut(s) 104, 991
TasI AATT 6 cut(s) 350, 456, 784, 1077, 1143, 1150
TatI WGTACW 2 cut(s) 415, 652
TauI GCSGC 2 cut(s) 617, 899
TfiI GAWTC 2 cut(s) 21, 979
Tru1I TTAA 5 cut(s) 33, 57, 386, 545, 942
Tru9I TTAA 5 cut(s) 33, 57, 386, 545, 942
TseFI GTSAC 3 cut(s) 151, 394, 502
Tsp45I GTSAC 3 cut(s) 151, 394, 502
TspDTI ATGAA 3 cut(s) 293, 554, 596
TspGWI ACGGA 4 cut(s) 108, 242, 517, 1175
VpaK11BI GGWCC 1 cut(s) 116
VspI ATTAAT 1 cut(s) 386
XagI CCTNNNNNAGG 1 cut(s) 486
XapI RAATTY 3 cut(s) 350, 1077, 1143
XceI RCATGY 1 cut(s) 266
XmaJI CCTAGG 1 cut(s) 344
XmiI GTMKAC 1 cut(s) 871
XspI CTAG 4 cut(s) 345, 471, 707, 1025
Zsp2I ATGCAT 2 cut(s) 177, 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.