Rh1BG203000

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0018296
Physical Location & Seq
Forward (+)
32047713 .. 32048672
960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG203000.1

Sequence Viewer

Length: 960 bp
ATGAGAGATGTTGAAGGGGGTTGGTTGCTCCGTTATATGCATGCTAATGGGGCAAGTATGTTTCTCATTGTGGTTCACCTTCATATTTTTCGCGGTCTATATCATGCGAGTTATAGCAGTCCTAGGGAATTTGTTTGGTGTCTCGGAGTTGTAATCTTCCTATTAATGATTGTGACAGCTTTTACAGGATACGTACTACCTTGGGGTCAGATGAGCTTTTGGGGAGCTACAGTAATTACAAGCTTAGCTAGCGCCATACCTGTAGTAGGGGATACCATAGTGACTTGGCTTTGGGGCGGTTTCTCCGTGGACAATGCCACCTTAAATCGTTTTTTTAGTCTTCATCATTTACTCCCCTTTATTTTAGTAGGCGCCAGTCTTCTTCATCTGGCCGCATTGCATCAATATGGATCAAATAATCCATTGGGTGTACATTCAGAGATGGATAAAATTTCTTTTTACCCTTATTTTTATGTAAAAGATCTAGTAGGTTGGGTAGCTTTTTCTATCTTTTCTTCCATTTGGATTTTTTATGCTCCTAATGTTTTGGGGCATCCCGACAATTATATACCTGCTAATCCGATGCCCACCCCGGCTCATATTGTGCCGGAATGGTATTTCCTACCGATCCATGCCATTCTTCGTAGTATACCTGACAAAGCGGGAGGTGTAGCCGCAATAGCACCAGTTTTTATATGTCTGTTGGCTTTACCTTTTTTTAAAAGTATGTATGTGCGTAGTTCAAGTTTTCGCCCTATTCACCAAGCAATGTTTTGGTTGCTTTTGGCGGATTGCATACTACTAGGTTGGATCGGATGTCAACCTGTGGAGGCACCATTTGTTACTATTGGACAAATTTCTCCTTTAGTTTTCTTCTTATTCTTTGCCATAACGCCCATTTCGGGAAAAGTTGGAAGAGGAATTCCTAATTCTTACACGGATGAGACTGATCACACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko02020 ko04260 ko04714 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map02020 map04260 map04714 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
Cytochrom_B_C Cytochrome_B

Protein Analysis

319

Amino Acids

35.64

Weight (kDa)

6.69

Isoelectric Point (pI)

38.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrome_B PF00033 1 - 137 7e-60 Cytochrome b/b6/petB
Cytochrom_B_N_2 PF13631 20 - 186 7.1e-44 Cytochrome b(N-terminal)/b6/petB
Cytochrom_B_C PF00032 191 - 292 5e-35 Cytochrome b(C-terminal)/b6/petD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018296)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07727 ATMG00220
rosa_chinensis RchiOBHm_MTg0499071
rosa_laevigata RLG00000000274 RLG00000000408 RLG00000000426 RLG00000000483
rosa_roxburghii Rroxscaffold_2G00119480
rosa_samantha Rh1BG203000 Rh1DG230000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 580
AccB1I GGYRCC 2 cut(s) 371, 832
AccI GTMKAC 1 cut(s) 649
AccII CGCG 1 cut(s) 93
AciI CCGC 6 cut(s) 93, 297, 393, 662, 675, 788
AclWI GGATC 3 cut(s) 418, 622, 818
AcoI YGGCCR 1 cut(s) 390
AcsI RAATTY 4 cut(s) 128, 450, 855, 921
AcyI GRCGYC 1 cut(s) 372
AfaI GTAC 2 cut(s) 195, 432
AfiI CCNNNNNNNGG 2 cut(s) 266, 902
AgsI TTSAA 2 cut(s) 14, 744
AluBI AGCT 6 cut(s) 179, 216, 227, 243, 248, 500
AluI AGCT 6 cut(s) 179, 216, 227, 243, 248, 500
Alw26I GTCTC 2 cut(s) 146, 938
AlwI GGATC 3 cut(s) 418, 622, 818
AoxI GGCC 1 cut(s) 390
ApoI RAATTY 4 cut(s) 128, 450, 855, 921
AseI ATTAAT 1 cut(s) 164
AspA2I CCTAGG 1 cut(s) 122
AspLEI GCGC 2 cut(s) 254, 374
AsuC2I CCSGG 1 cut(s) 593
AsuHPI GGTGA 2 cut(s) 68, 752
AsuNHI GCTAGC 1 cut(s) 248
AvrII CCTAGG 1 cut(s) 122
BaeI ACNNNNGTAYC 2 cut(s) 264, 297
BanI GGYRCC 2 cut(s) 371, 832
BbsI GAAGAC 2 cut(s) 332, 371
BccI CCATC 1 cut(s) 436
BciVI GTATCC 2 cut(s) 182, 265
BclI TGATCA 1 cut(s) 949
BcnI CCSGG 1 cut(s) 593
BcoDI GTCTC 2 cut(s) 146, 938
BfaI CTAG 4 cut(s) 123, 249, 485, 803
BfmI CTRYAG 2 cut(s) 228, 261
BfoI RGCGCY 2 cut(s) 255, 375
BfuAI ACCTGC 1 cut(s) 580
BfuI GTATCC 2 cut(s) 182, 265
BglII AGATCT 1 cut(s) 481
BisI GCNGC 2 cut(s) 393, 675
BlnI CCTAGG 1 cut(s) 122
BlpI GCTNAGC 1 cut(s) 244
BlsI GCNGC 2 cut(s) 394, 676
Bme1390I CCNGG 1 cut(s) 593
BmiI GGNNCC 2 cut(s) 373, 834
BmrFI CCNGG 1 cut(s) 593
BmsI GCATC 3 cut(s) 409, 562, 573
BmtI GCTAGC 1 cut(s) 252
BpiI GAAGAC 2 cut(s) 332, 371
Bpu1102I GCTNAGC 1 cut(s) 244
BpuMI CCSGG 1 cut(s) 593
BsaAI YACGTR 1 cut(s) 193
BsaHI GRCGYC 1 cut(s) 372
BsaJI CCNNGG 4 cut(s) 122, 200, 306, 591
BsaXI ACNNNNNCTCC 2 cut(s) 216, 246
Bsc4I CCNNNNNNNGG 2 cut(s) 266, 902
Bse1I ACTGG 2 cut(s) 375, 686
Bse3DI GCAATG 2 cut(s) 395, 774
BseDI CCNNGG 4 cut(s) 122, 200, 306, 591
BseGI GGATG 3 cut(s) 553, 821, 946
BseLI CCNNNNNNNGG 2 cut(s) 266, 902
BseMI GCAATG 2 cut(s) 395, 774
BseNI ACTGG 2 cut(s) 375, 686
Bsh1236I CGCG 1 cut(s) 93
BshFI GGCC 1 cut(s) 392
BshNI GGYRCC 2 cut(s) 371, 832
BsiSI CCGG 2 cut(s) 593, 608
BslI CCNNNNNNNGG 2 cut(s) 266, 902
BsmAI GTCTC 2 cut(s) 146, 938
BsnI GGCC 1 cut(s) 392
Bsp1407I TGTACA 1 cut(s) 430
Bsp143I GATC 5 cut(s) 410, 481, 627, 810, 949
Bsp1720I GCTNAGC 1 cut(s) 244
BspACI CCGC 6 cut(s) 93, 297, 393, 662, 675, 788
BspANI GGCC 1 cut(s) 392
BspFNI CGCG 1 cut(s) 93
BspLI GGNNCC 2 cut(s) 373, 834
BspMI ACCTGC 1 cut(s) 580
BspOI GCTAGC 1 cut(s) 252
BspPI GGATC 3 cut(s) 418, 622, 818
BspT107I GGYRCC 2 cut(s) 371, 832
BsrDI GCAATG 2 cut(s) 395, 774
BsrGI TGTACA 1 cut(s) 430
BsrI ACTGG 2 cut(s) 375, 686
BssECI CCNNGG 4 cut(s) 122, 200, 306, 591
BssMI GATC 5 cut(s) 410, 481, 627, 810, 949
BssNAI GTATAC 1 cut(s) 650
BssNI GRCGYC 1 cut(s) 372
BssT1I CCWWGG 2 cut(s) 122, 200
Bst1107I GTATAC 1 cut(s) 650
Bst4CI ACNGT 1 cut(s) 232
Bst6I CTCTTC 1 cut(s) 910
BstACI GRCGYC 1 cut(s) 372
BstAUI TGTACA 1 cut(s) 430
BstBAI YACGTR 1 cut(s) 193
BstC8I GCNNGC 2 cut(s) 42, 250
BstDEI CTNAG 1 cut(s) 244
BstDSI CCRYGG 1 cut(s) 306
BstENI CCTNNNNNAGG 1 cut(s) 264
BstF5I GGATG 3 cut(s) 553, 821, 946
BstFNI CGCG 1 cut(s) 93
BstH2I RGCGCY 2 cut(s) 255, 375
BstHHI GCGC 2 cut(s) 254, 374
BstKTI GATC 5 cut(s) 413, 484, 630, 813, 952
BstMAI GTCTC 2 cut(s) 146, 938
BstMBI GATC 5 cut(s) 410, 481, 627, 810, 949
BstMWI GCNNNNNNNGC 3 cut(s) 50, 249, 680
BstNSI RCATGY 1 cut(s) 44
BstSCI CCNGG 1 cut(s) 591
BstSFI CTRYAG 2 cut(s) 228, 261
BstSNI TACGTA 1 cut(s) 193
BstUI CGCG 1 cut(s) 93
BstV2I GAAGAC 2 cut(s) 332, 371
BstX2I RGATCY 1 cut(s) 481
BstYI RGATCY 1 cut(s) 481
BstZ17I GTATAC 1 cut(s) 650
BsuI GTATCC 2 cut(s) 182, 265
BsuRI GGCC 1 cut(s) 392
BtgI CCRYGG 1 cut(s) 306
BtsCI GGATG 3 cut(s) 553, 821, 946
BveI ACCTGC 1 cut(s) 580
Cac8I GCNNGC 2 cut(s) 42, 250
CfoI GCGC 2 cut(s) 254, 374
Csp6I GTAC 2 cut(s) 194, 431
CviAII CATG 3 cut(s) 41, 104, 632
CviQI GTAC 2 cut(s) 194, 431
DdeI CTNAG 1 cut(s) 244
DinI GGCGCC 1 cut(s) 373
DpnI GATC 5 cut(s) 412, 483, 629, 812, 951
DpnII GATC 5 cut(s) 410, 481, 627, 810, 949
DraI TTTAAA 1 cut(s) 721
EaeI YGGCCR 1 cut(s) 390
Eam1104I CTCTTC 1 cut(s) 910
EarI CTCTTC 1 cut(s) 910
EciI GGCGGA 1 cut(s) 803
Eco105I TACGTA 1 cut(s) 193
Eco130I CCWWGG 2 cut(s) 122, 200
EcoNI CCTNNNNNAGG 1 cut(s) 264
EcoRI GAATTC 1 cut(s) 921
EcoT14I CCWWGG 2 cut(s) 122, 200
EcoT22I ATGCAT 1 cut(s) 42
EgeI GGCGCC 1 cut(s) 373
EheI GGCGCC 1 cut(s) 373
ErhI CCWWGG 2 cut(s) 122, 200
FaeI CATG 3 cut(s) 44, 107, 635
FatI CATG 3 cut(s) 40, 103, 631
FauI CCCGC 1 cut(s) 655
FbaI TGATCA 1 cut(s) 949
FblI GTMKAC 1 cut(s) 649
Fnu4HI GCNGC 2 cut(s) 393, 675
FokI GGATG 3 cut(s) 540, 828, 953
Fsp4HI GCNGC 2 cut(s) 393, 675
FspBI CTAG 4 cut(s) 123, 249, 485, 803
GlaI GCGC 2 cut(s) 253, 373
GluI GCNGC 2 cut(s) 393, 675
HaeII RGCGCY 2 cut(s) 255, 375
HaeIII GGCC 1 cut(s) 392
HapII CCGG 2 cut(s) 593, 608
HhaI GCGC 2 cut(s) 254, 374
Hin1I GRCGYC 1 cut(s) 372
Hin1II CATG 3 cut(s) 44, 107, 635
Hin6I GCGC 2 cut(s) 252, 372
HinP1I GCGC 2 cut(s) 252, 372
HincII GTYRAC 1 cut(s) 821
HindII GTYRAC 1 cut(s) 821
HindIII AAGCTT 1 cut(s) 241
HpaII CCGG 2 cut(s) 593, 608
HphI GGTGA 2 cut(s) 68, 752
Hpy166II GTNNAC 5 cut(s) 76, 310, 431, 650, 821
Hpy188I TCNGA 5 cut(s) 146, 210, 439, 582, 815
Hpy188III TCNNGA 2 cut(s) 557, 903
Hpy8I GTNNAC 5 cut(s) 76, 310, 431, 650, 821
HpyAV CCTTC 2 cut(s) 8, 89
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 3 cut(s) 40, 400, 795
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 249, 680
HpyF3I CTNAG 1 cut(s) 244
HpySE526I ACGT 1 cut(s) 192
Hsp92I GRCGYC 1 cut(s) 372
Hsp92II CATG 3 cut(s) 44, 107, 635
HspAI GCGC 2 cut(s) 252, 372
KasI GGCGCC 1 cut(s) 371
Ksp22I TGATCA 1 cut(s) 949
Kzo9I GATC 5 cut(s) 410, 481, 627, 810, 949
LmnI GCTCC 3 cut(s) 33, 224, 541
LweI GCATC 3 cut(s) 409, 562, 573
MaeI CTAG 4 cut(s) 123, 249, 485, 803
MaeII ACGT 1 cut(s) 192
MaeIII GTNAC 3 cut(s) 172, 280, 841
MalI GATC 5 cut(s) 412, 483, 629, 812, 951
MboI GATC 5 cut(s) 410, 481, 627, 810, 949
MboII GAAGA 8 cut(s) 148, 332, 371, 374, 507, 632, 865, 927
MflI RGATCY 1 cut(s) 481
MluCI AATT 7 cut(s) 128, 234, 450, 562, 855, 921, 928
Mly113I GGCGCC 1 cut(s) 372
MmeI TCCRAC 2 cut(s) 788, 892
MnlI CCTC 3 cut(s) 659, 823, 911
Mph1103I ATGCAT 1 cut(s) 42
MseI TTAA 3 cut(s) 164, 323, 720
MslI CAYNNNNRTG 2 cut(s) 45, 405
MspI CCGG 2 cut(s) 593, 608
MspR9I CCNGG 1 cut(s) 593
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 3 cut(s) 50, 249, 680
NarI GGCGCC 1 cut(s) 372
NciI CCSGG 1 cut(s) 593
NdeII GATC 5 cut(s) 410, 481, 627, 810, 949
NheI GCTAGC 1 cut(s) 248
NlaIII CATG 3 cut(s) 44, 107, 635
NlaIV GGNNCC 2 cut(s) 373, 834
NmuCI GTSAC 2 cut(s) 172, 280
NsiI ATGCAT 1 cut(s) 42
NspI RCATGY 1 cut(s) 44
PaeI GCATGC 1 cut(s) 44
PkrI GCNGC 2 cut(s) 394, 676
PluTI GGCGCC 1 cut(s) 375
Ppu21I YACGTR 1 cut(s) 193
PshBI ATTAAT 1 cut(s) 164
PspN4I GGNNCC 2 cut(s) 373, 834
PsuI RGATCY 1 cut(s) 481
RsaI GTAC 2 cut(s) 195, 432
RsaNI GTAC 2 cut(s) 194, 431
RseI CAYNNNNRTG 2 cut(s) 45, 405
SaqAI TTAA 3 cut(s) 164, 323, 720
SatI GCNGC 2 cut(s) 393, 675
Sau3AI GATC 5 cut(s) 410, 481, 627, 810, 949
ScrFI CCNGG 1 cut(s) 593
SfaNI GCATC 3 cut(s) 409, 562, 573
SfcI CTRYAG 2 cut(s) 228, 261
SfoI GGCGCC 1 cut(s) 373
SmiMI CAYNNNNRTG 2 cut(s) 45, 405
SnaBI TACGTA 1 cut(s) 193
SphI GCATGC 1 cut(s) 44
Sse9I AATT 7 cut(s) 128, 234, 450, 562, 855, 921, 928
SsiI CCGC 6 cut(s) 93, 297, 393, 662, 675, 788
SspDI GGCGCC 1 cut(s) 371
SspMI CTAG 4 cut(s) 123, 249, 485, 803
StyD4I CCNGG 1 cut(s) 591
StyI CCWWGG 2 cut(s) 122, 200
TaaI ACNGT 1 cut(s) 232
TaiI ACGT 1 cut(s) 195
TasI AATT 7 cut(s) 128, 234, 450, 562, 855, 921, 928
TatI WGTACW 1 cut(s) 430
TauI GCSGC 2 cut(s) 395, 677
Tru1I TTAA 3 cut(s) 164, 323, 720
Tru9I TTAA 3 cut(s) 164, 323, 720
TseFI GTSAC 2 cut(s) 172, 280
Tsp45I GTSAC 2 cut(s) 172, 280
TspDTI ATGAA 3 cut(s) 71, 332, 374
TspGWI ACGGA 3 cut(s) 20, 295, 953
VspI ATTAAT 1 cut(s) 164
XagI CCTNNNNNAGG 1 cut(s) 264
XapI RAATTY 4 cut(s) 128, 450, 855, 921
XceI RCATGY 1 cut(s) 44
XmaJI CCTAGG 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 649
XspI CTAG 4 cut(s) 123, 249, 485, 803
Zsp2I ATGCAT 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.