AT2G25840

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006801
Physical Location & Seq
Forward (+)
11021692 .. 11025451
3760 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G25840.1

Sequence Viewer

Length: 1227 bp
ATGGGACACGCCACGAGTCTCTCTCACTTCCTCATCCTCTCTTCCTCCAGGTTCTCTCGTCTCGGGTCTCTCACTAGATTACTCTCCAAACCCACCTCTCTCTCCGGCTCTTTTTCTTCCATTTCGGTCACCGGACAAGGGTTCCGATGCTGTTGCTCCGTCGCCACAGATGACACTTCTCCTTCTGTAAAGAAACGTGTGGTCTCTGGTGTCCAGCCTACAGGTTCAGTTCACTTAGGCAACTATCTCGGGGCTATCAAAAACTGGGTCGCTCTTCAGGATACATATGAAACACTCTTTATCATCGTAGACCATCACGCGATAACACTTCCATATGATACACGTCAACTAGGAAAGGCAACAACGGATACCGCAGCACTTTATCTAGCATGTGGTATAGACGTCTCTAAGGCTTCAGTATTTGTGCAATCTCACGTTCCCGCTCATGTCGAGTTAATGTGGCTTTTATGTTCATCCACACCTATTGGGTGGTTACAGAAAATGATTCAGTTCAAAGAGAAATCACGCAAGGAGGGGGTTGAGAATGCTAGTGTCGGTTTGTTTACTTATCCTGATCTGATGACTGCTGATATCCTCTTGTATCAGTCTGATTTTGTCCCTGTTGGTGAGGACCAGAAGCAGCACATAGAACTTGCCCGTGAAATTGCACAGCGTGTGAATCATTTATATGGTGGAAAGAAGTGGAAGAAGCTTGGAGGCCGCGGTGGTTCGCTCTTTAAGATACCAGAACCACTCATACCACAAGCTGGAGCTCGTGTTATGTCTCTTACTGATGGTCTTTCCAAGATGTCCAAGTCTGCCCCTTCTGATCAGTCCCGGATTAATCTCCTTGACTCAAAAGATTTGATTGTGGACAAGATAAAACGGTGCAAGACAGACTCATTTGCTGGGCTTGAATTTGACAATGCTGAGAGACCTGAATGCAACAATCTCCTCTCAATATATCAGATTGTTTCAGGCAAGAAAAAAGAGGAAGTGATGGAGGAATGCAAAGATATGAGCTGGGGTACATTTAAGCCTCTCCTTGCAGATGCTTTGATCGAACATCTGAGTCCAATCCAGGCCCGTTATCAGGAGATAATAGCGGAACCAGAGTATTTGGACAAAATATTGTCAGAAGGTGCGGATAGAGCCGAGGAGTTGGGAGCTGTCACAATGCGCAATATGTATCAAGCTATGGGATACTATCAGAGGAGGAGATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
tRNA-synt_1b

Protein Analysis

408

Amino Acids

45.3

Weight (kDa)

8.29

Isoelectric Point (pI)

43.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1b PF00579 65 - 357 3.3e-83 tRNA synthetases class I (W and Y)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 405
Acc16I TGCGCA 1 cut(s) 1181
AccB7I CCANNNNNTGG 1 cut(s) 767
AccBSI CCGCTC 1 cut(s) 443
AccI GTMKAC 1 cut(s) 309
AccII CGCG 2 cut(s) 320, 723
AciI CCGC 6 cut(s) 372, 441, 721, 723, 1106, 1145
AcsI RAATTY 1 cut(s) 917
AcuI CTGAAG 2 cut(s) 260, 399
AcyI GRCGYC 1 cut(s) 402
AdeI CACNNNGTG 1 cut(s) 674
AfaI GTAC 1 cut(s) 1030
AfiI CCNNNNNNNGG 3 cut(s) 138, 767, 1093
AflIII ACRYGT 2 cut(s) 196, 341
AgsI TTSAA 2 cut(s) 514, 917
AjiI CACGTC 1 cut(s) 344
AjnI CCWGG 2 cut(s) 47, 1080
AluBI AGCT 6 cut(s) 712, 767, 773, 1023, 1169, 1196
AluI AGCT 6 cut(s) 712, 767, 773, 1023, 1169, 1196
Alw21I GWGCWC 1 cut(s) 775
Alw26I GTCTC 7 cut(s) 23, 65, 72, 208, 409, 789, 928
Ama87I CYCGRG 2 cut(s) 62, 248
AoxI GGCC 2 cut(s) 718, 1083
ApeKI GCWGC 2 cut(s) 374, 640
ApoI RAATTY 1 cut(s) 917
ArsI GACNNNNNNTTYG 2 cut(s) 252, 284
AseI ATTAAT 1 cut(s) 843
AspLEI GCGC 1 cut(s) 1182
AspS9I GGNCC 2 cut(s) 631, 1084
AsuC2I CCSGG 1 cut(s) 838
AsuHPI GGTGA 2 cut(s) 121, 638
AvaI CYCGRG 2 cut(s) 62, 248
AvaII GGWCC 1 cut(s) 631
BanII GRGCYC 1 cut(s) 775
BauI CACGAG 2 cut(s) 13, 774
Bbv12I GWGCWC 1 cut(s) 775
BbvI GCAGC 2 cut(s) 386, 652
BccI CCATC 3 cut(s) 321, 788, 994
BcgI CGANNNNNNTGC 4 cut(s) 135, 169, 229, 263
BciT130I CCWGG 2 cut(s) 49, 1082
BciVI GTATCC 3 cut(s) 274, 361, 1196
BclI TGATCA 1 cut(s) 829
BcnI CCSGG 1 cut(s) 838
BcoDI GTCTC 7 cut(s) 23, 65, 72, 208, 409, 789, 928
BfaI CTAG 4 cut(s) 75, 350, 386, 549
BfmI CTRYAG 1 cut(s) 219
BfuI GTATCC 3 cut(s) 274, 361, 1196
BisI GCNGC 3 cut(s) 375, 641, 721
BlsI GCNGC 3 cut(s) 376, 642, 722
Bme1390I CCNGG 3 cut(s) 49, 838, 1082
Bme18I GGWCC 1 cut(s) 631
BmeT110I CYCGRG 2 cut(s) 62, 248
BmgBI CACGTC 1 cut(s) 344
BmgT120I GGNCC 2 cut(s) 631, 1084
BmiI GGNNCC 2 cut(s) 143, 1110
BmrFI CCNGG 3 cut(s) 49, 838, 1082
BmrI ACTGGG 1 cut(s) 274
BmsI GCATC 2 cut(s) 137, 1042
BmuI ACTGGG 1 cut(s) 274
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpmI CTGGAG 2 cut(s) 31, 789
BpuMI CCSGG 1 cut(s) 838
BsaHI GRCGYC 1 cut(s) 402
BsaI GGTCTC 3 cut(s) 72, 208, 928
BsaJI CCNNGG 2 cut(s) 721, 1155
BsaWI WCCGGW 1 cut(s) 131
BsaXI ACNNNNNCTCC 2 cut(s) 762, 792
Bsc4I CCNNNNNNNGG 3 cut(s) 138, 767, 1093
Bse1I ACTGG 1 cut(s) 269
BseBI CCWGG 2 cut(s) 49, 1082
BseDI CCNNGG 2 cut(s) 721, 1155
BseGI GGATG 2 cut(s) 33, 473
BseLI CCNNNNNNNGG 3 cut(s) 138, 767, 1093
BseMII CTCAG 2 cut(s) 921, 1061
BseNI ACTGG 1 cut(s) 269
BseRI GAGGAG 2 cut(s) 944, 1172
BseXI GCAGC 2 cut(s) 386, 652
BseYI CCCAGC 2 cut(s) 908, 1023
Bsh1236I CGCG 2 cut(s) 320, 723
BshFI GGCC 2 cut(s) 720, 1085
BsiHKAI GWGCWC 1 cut(s) 775
BsiHKCI CYCGRG 2 cut(s) 62, 248
BsiSI CCGG 3 cut(s) 105, 132, 838
BslFI GGGAC 3 cut(s) 18, 602, 820
BslI CCNNNNNNNGG 3 cut(s) 138, 767, 1093
BsmAI GTCTC 7 cut(s) 23, 65, 72, 208, 409, 789, 928
BsmBI CGTCTC 2 cut(s) 65, 409
BsmFI GGGAC 3 cut(s) 18, 602, 820
BsmI GAATGC 3 cut(s) 550, 947, 1013
BsnI GGCC 2 cut(s) 720, 1085
Bso31I GGTCTC 3 cut(s) 72, 208, 928
BsoBI CYCGRG 2 cut(s) 62, 248
Bsp1286I GDGCHC 1 cut(s) 775
Bsp143I GATC 3 cut(s) 574, 829, 1059
BspACI CCGC 6 cut(s) 372, 441, 721, 723, 1106, 1145
BspANI GGCC 2 cut(s) 720, 1085
BspCNI CTCAG 2 cut(s) 922, 1062
BspFNI CGCG 2 cut(s) 320, 723
BspLI GGNNCC 2 cut(s) 143, 1110
BspQI GCTCTTC 1 cut(s) 279
BspTNI GGTCTC 3 cut(s) 72, 208, 928
BsrBI CCGCTC 1 cut(s) 443
BsrI ACTGG 1 cut(s) 269
BssECI CCNNGG 2 cut(s) 721, 1155
BssMI GATC 3 cut(s) 574, 829, 1059
BssNI GRCGYC 1 cut(s) 402
BssSI CACGAG 2 cut(s) 13, 774
Bst2BI CACGAG 2 cut(s) 13, 774
Bst2UI CCWGG 2 cut(s) 49, 1082
Bst4CI ACNGT 1 cut(s) 888
Bst6I CTCTTC 2 cut(s) 46, 279
BstACI GRCGYC 1 cut(s) 402
BstDEI CTNAG 4 cut(s) 235, 408, 930, 1070
BstDSI CCRYGG 1 cut(s) 721
BstEII GGTNACC 1 cut(s) 127
BstF5I GGATG 2 cut(s) 33, 473
BstFNI CGCG 2 cut(s) 320, 723
BstHHI GCGC 1 cut(s) 1182
BstKTI GATC 3 cut(s) 577, 832, 1062
BstMAI GTCTC 7 cut(s) 23, 65, 72, 208, 409, 789, 928
BstMBI GATC 3 cut(s) 574, 829, 1059
BstMWI GCNNNNNNNGC 1 cut(s) 1151
BstNI CCWGG 2 cut(s) 49, 1082
BstNSI RCATGY 1 cut(s) 393
BstPI GGTNACC 1 cut(s) 127
BstSCI CCNGG 3 cut(s) 47, 836, 1080
BstSFI CTRYAG 1 cut(s) 219
BstUI CGCG 2 cut(s) 320, 723
BstV1I GCAGC 2 cut(s) 386, 652
BsuI GTATCC 3 cut(s) 274, 361, 1196
BsuRI GGCC 2 cut(s) 720, 1085
BtgI CCRYGG 1 cut(s) 721
BtrI CACGTC 1 cut(s) 344
BtsCI GGATG 2 cut(s) 33, 473
CfoI GCGC 1 cut(s) 1182
Cfr13I GGNCC 2 cut(s) 631, 1084
Cfr42I CCGCGG 1 cut(s) 724
Csp6I GTAC 1 cut(s) 1029
CspCI CAANNNNNGTGG 2 cut(s) 466, 501
CviAII CATG 2 cut(s) 390, 446
CviQI GTAC 1 cut(s) 1029
DdeI CTNAG 4 cut(s) 235, 408, 930, 1070
DpnI GATC 3 cut(s) 576, 831, 1061
DpnII GATC 3 cut(s) 574, 829, 1059
DraIII CACNNNGTG 1 cut(s) 674
Eam1104I CTCTTC 2 cut(s) 46, 279
EarI CTCTTC 2 cut(s) 46, 279
Ecl136II GAGCTC 1 cut(s) 773
Eco24I GRGCYC 1 cut(s) 775
Eco31I GGTCTC 3 cut(s) 72, 208, 928
Eco32I GATATC 1 cut(s) 592
Eco47I GGWCC 1 cut(s) 631
Eco53kI GAGCTC 1 cut(s) 773
Eco57I CTGAAG 2 cut(s) 260, 399
Eco88I CYCGRG 2 cut(s) 62, 248
Eco91I GGTNACC 1 cut(s) 127
EcoICRI GAGCTC 1 cut(s) 773
EcoO65I GGTNACC 1 cut(s) 127
EcoRII CCWGG 2 cut(s) 47, 1080
EcoRV GATATC 1 cut(s) 592
EcoT38I GRGCYC 1 cut(s) 775
Esp3I CGTCTC 2 cut(s) 65, 409
FaeI CATG 2 cut(s) 393, 449
FaqI GGGAC 3 cut(s) 18, 602, 820
FatI CATG 2 cut(s) 389, 445
FauI CCCGC 1 cut(s) 448
FauNDI CATATG 2 cut(s) 286, 334
FbaI TGATCA 1 cut(s) 829
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 3 cut(s) 375, 641, 721
FokI GGATG 2 cut(s) 20, 460
FriOI GRGCYC 1 cut(s) 775
Fsp4HI GCNGC 3 cut(s) 375, 641, 721
FspBI CTAG 4 cut(s) 75, 350, 386, 549
FspI TGCGCA 1 cut(s) 1181
GlaI GCGC 1 cut(s) 1181
GluI GCNGC 3 cut(s) 375, 641, 721
GsaI CCCAGC 2 cut(s) 912, 1027
GsuI CTGGAG 2 cut(s) 31, 789
HaeIII GGCC 2 cut(s) 720, 1085
HapII CCGG 3 cut(s) 105, 132, 838
HhaI GCGC 1 cut(s) 1182
Hin1I GRCGYC 1 cut(s) 402
Hin1II CATG 2 cut(s) 393, 449
Hin6I GCGC 1 cut(s) 1180
HinP1I GCGC 1 cut(s) 1180
HincII GTYRAC 1 cut(s) 347
HindII GTYRAC 1 cut(s) 347
HindIII AAGCTT 1 cut(s) 710
HinfI GANTC 6 cut(s) 16, 505, 679, 854, 899, 1072
HpaII CCGG 3 cut(s) 105, 132, 838
HphI GGTGA 2 cut(s) 121, 638
Hpy166II GTNNAC 5 cut(s) 232, 310, 347, 564, 874
Hpy188I TCNGA 8 cut(s) 146, 579, 610, 829, 969, 1071, 1138, 1212
Hpy188III TCNNGA 3 cut(s) 278, 572, 1094
Hpy8I GTNNAC 5 cut(s) 232, 310, 347, 564, 874
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 3 cut(s) 192, 834, 1133
HpyCH4III ACNGT 1 cut(s) 888
HpyCH4IV ACGT 4 cut(s) 196, 343, 402, 435
HpyCH4V TGCA 6 cut(s) 427, 668, 891, 945, 1011, 1049
HpyF10VI GCNNNNNNNGC 1 cut(s) 1151
HpyF3I CTNAG 4 cut(s) 235, 408, 930, 1070
HpySE526I ACGT 4 cut(s) 196, 343, 402, 435
Hsp92I GRCGYC 1 cut(s) 402
Hsp92II CATG 2 cut(s) 393, 449
HspAI GCGC 1 cut(s) 1180
Ksp22I TGATCA 1 cut(s) 829
KspI CCGCGG 1 cut(s) 724
Kzo9I GATC 3 cut(s) 574, 829, 1059
LguI GCTCTTC 1 cut(s) 279
LmnI GCTCC 3 cut(s) 161, 770, 1166
Lsp1109I GCAGC 2 cut(s) 386, 652
LweI GCATC 2 cut(s) 137, 1042
MaeI CTAG 4 cut(s) 75, 350, 386, 549
MaeII ACGT 4 cut(s) 196, 343, 402, 435
MaeIII GTNAC 3 cut(s) 127, 492, 1171
MalI GATC 3 cut(s) 576, 831, 1061
MbiI CCGCTC 1 cut(s) 443
MboI GATC 3 cut(s) 574, 829, 1059
MboII GAAGA 4 cut(s) 33, 108, 266, 718
MhlI GDGCHC 1 cut(s) 775
MluCI AATT 2 cut(s) 663, 917
MlyI GAGTC 4 cut(s) 25, 848, 893, 1081
MseI TTAA 4 cut(s) 455, 738, 843, 1035
MslI CAYNNNNRTG 1 cut(s) 687
MspA1I CMGCKG 1 cut(s) 723
MspI CCGG 3 cut(s) 105, 132, 838
MspR9I CCNGG 3 cut(s) 49, 838, 1082
Mva1269I GAATGC 3 cut(s) 550, 947, 1013
MvaI CCWGG 2 cut(s) 49, 1082
MvnI CGCG 2 cut(s) 320, 723
MwoI GCNNNNNNNGC 1 cut(s) 1151
NciI CCSGG 1 cut(s) 838
NdeI CATATG 2 cut(s) 286, 334
NdeII GATC 3 cut(s) 574, 829, 1059
NlaIII CATG 2 cut(s) 393, 449
NlaIV GGNNCC 2 cut(s) 143, 1110
NmeAIII GCCGAG 1 cut(s) 1180
NmuCI GTSAC 2 cut(s) 127, 1171
NsbI TGCGCA 1 cut(s) 1181
NspI RCATGY 1 cut(s) 393
PciSI GCTCTTC 1 cut(s) 279
PctI GAATGC 3 cut(s) 550, 947, 1013
PfeI GAWTC 2 cut(s) 505, 679
PflMI CCANNNNNTGG 1 cut(s) 767
PfoI TCCNGGA 1 cut(s) 836
PkrI GCNGC 3 cut(s) 376, 642, 722
PleI GAGTC 4 cut(s) 24, 848, 893, 1080
PpsI GAGTC 4 cut(s) 24, 848, 893, 1080
PshBI ATTAAT 1 cut(s) 843
Psp124BI GAGCTC 1 cut(s) 775
Psp6I CCWGG 2 cut(s) 47, 1080
PspEI GGTNACC 1 cut(s) 127
PspFI CCCAGC 2 cut(s) 908, 1023
PspGI CCWGG 2 cut(s) 47, 1080
PspN4I GGNNCC 2 cut(s) 143, 1110
PspPI GGNCC 2 cut(s) 631, 1084
PsrI GAACNNNNNNTAC 2 cut(s) 741, 773
RsaI GTAC 1 cut(s) 1030
RsaNI GTAC 1 cut(s) 1029
RseI CAYNNNNRTG 1 cut(s) 687
SacI GAGCTC 1 cut(s) 775
SacII CCGCGG 1 cut(s) 724
SapI GCTCTTC 1 cut(s) 279
SaqAI TTAA 4 cut(s) 455, 738, 843, 1035
SatI GCNGC 3 cut(s) 375, 641, 721
Sau3AI GATC 3 cut(s) 574, 829, 1059
Sau96I GGNCC 2 cut(s) 631, 1084
SchI GAGTC 4 cut(s) 25, 848, 893, 1081
ScrFI CCNGG 3 cut(s) 49, 838, 1082
SduI GDGCHC 1 cut(s) 775
SfaNI GCATC 2 cut(s) 137, 1042
SfcI CTRYAG 1 cut(s) 219
Sfr303I CCGCGG 1 cut(s) 724
SgrBI CCGCGG 1 cut(s) 724
SinI GGWCC 1 cut(s) 631
SmiMI CAYNNNNRTG 1 cut(s) 687
Sse9I AATT 2 cut(s) 663, 917
SsiI CCGC 6 cut(s) 372, 441, 721, 723, 1106, 1145
SspI AATATT 1 cut(s) 1131
SspMI CTAG 4 cut(s) 75, 350, 386, 549
SstI GAGCTC 1 cut(s) 775
StyD4I CCNGG 3 cut(s) 47, 836, 1080
TaaI ACNGT 1 cut(s) 888
TaiI ACGT 4 cut(s) 199, 346, 405, 438
TaqI TCGA 2 cut(s) 450, 1062
TaqII GACCGA 1 cut(s) 115
TasI AATT 2 cut(s) 663, 917
TauI GCSGC 1 cut(s) 723
TfiI GAWTC 2 cut(s) 505, 679
Tru1I TTAA 4 cut(s) 455, 738, 843, 1035
Tru9I TTAA 4 cut(s) 455, 738, 843, 1035
TseFI GTSAC 2 cut(s) 127, 1171
TseI GCWGC 2 cut(s) 374, 640
Tsp45I GTSAC 2 cut(s) 127, 1171
TspDTI ATGAA 2 cut(s) 303, 462
TspGWI ACGGA 2 cut(s) 148, 380
Van91I CCANNNNNTGG 1 cut(s) 767
VpaK11BI GGWCC 1 cut(s) 631
VspI ATTAAT 1 cut(s) 843
XapI RAATTY 1 cut(s) 917
XceI RCATGY 1 cut(s) 393
XmiI GTMKAC 1 cut(s) 309
XspI CTAG 4 cut(s) 75, 350, 386, 549
ZraI GACGTC 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.