Rorug06G0351100

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001440
Physical Location & Seq
Reverse (-)
50018247 .. 50021670
3424 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0351100.1

Sequence Viewer

Length: 981 bp
ATGAAGTTCTGCAAGAAATACCAGGAGTACATGCAAGGCCAAGGGAAGAAGCTACCTGGGGTTGGCTTCAAGAAGCTTAAGAAGATCTTGAAGAAATGCCGCAGAGATTTTCAGTCCGGAAATGACATTAATAATCATGCCGTACATGATCCCAGAACATGCCCTGACCATTGCCCAGTGTGTGATGGGTCGTTTTTCCCTTCTCTGCTCAATGAAATGTCTGCAGTGGTAGGCTTTTTTAACCAGAAGGCACAGAAATTGCTCGAGTACCATTTGGCTTCTGGGTTTCGCAAGTACATGCTTGTGTTCAAAGGCAAGCCTCGAGGAAACCATGTTGCCTTAATCCAAGAAGGCAAGGACCTTGTCACTTATGCTCTCATTAACGCCATTGCGATTCGAAAAATACTCAAGAAATATGACAAGATTCATTACTCAAAGCAAGGGCAGGCCTTTAAGTCACAAGCGCAAAGTATGCACATTGGAATTCTTCAAAGTCCTTGGCTTTGTGAGCTTATGGCTTTCCACATCAATTTAAGGCAATCGAAGATCAAGTCGGGGAAGAGACCGGCGTTGTTTGAGGGATGCTCTCTTACAATGAATGATGAGAAGCCGTCTCTCACCTGTGAGCTCTTTGATTCTGTCAAAGTTGATATTGACTTGACATGTTCTATATGCCTGGACACAGTTTTTGATCCAGTTGCTCTCACTTGCGGTCATATATTCTGCTACCTATGTGCTTGCTCGGCTGCATCAGTGAACATTGTCGATGGGTTAAAGGCTGCTGAGCCTAAAGAAAAATGCCCTTTATGCCGAGAGGCAAAAGTTTACCAAGGTTCTGTACACCTAGAAGAGCTTAGCATTTTACTCAGCAGAAGCTGCCGCGAATACTGGGAGCAGCGGATTCAGACAGAAAAGGTGGAGAGGGTTCGGCTAGCAAAGCAGCACTGGGAATCACAGTGCCGGGCATTCATGGGCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
tRNA-synt_1b

Protein Analysis

326

Amino Acids

37.07

Weight (kDa)

9.15

Isoelectric Point (pI)

46.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RING_XB3-XBAT31 PF24921 221 - 274 6.2e-08 E3 ubiquitin-protein ligase XB3/XBAT31 RING finger
zf-C3HC4 PF00097 222 - 270 1.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 222 - 250 1.9e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 321
AccII CGCG 1 cut(s) 882
AccIII TCCGGA 1 cut(s) 116
AciI CCGC 4 cut(s) 100, 711, 880, 898
AclWI GGATC 2 cut(s) 143, 686
AcsI RAATTY 1 cut(s) 483
AfaI GTAC 5 cut(s) 29, 144, 269, 296, 840
AfiI CCNNNNNNNGG 1 cut(s) 62
AflII CTTAAG 1 cut(s) 77
AflIII ACRYGT 1 cut(s) 662
AgsI TTSAA 4 cut(s) 70, 91, 310, 491
AjnI CCWGG 3 cut(s) 21, 55, 675
AluBI AGCT 6 cut(s) 52, 76, 511, 628, 853, 876
AluI AGCT 6 cut(s) 52, 76, 511, 628, 853, 876
Alw21I GWGCWC 1 cut(s) 630
Alw26I GTCTC 2 cut(s) 556, 618
AlwI GGATC 2 cut(s) 143, 686
AlwNI CAGNNNCTG 1 cut(s) 876
Ama87I CYCGRG 2 cut(s) 263, 321
Aor13HI TCCGGA 1 cut(s) 116
AoxI GGCC 2 cut(s) 37, 447
ApeKI GCWGC 5 cut(s) 746, 779, 876, 895, 940
ApoI RAATTY 1 cut(s) 483
ArsI GACNNNNNNTTYG 4 cut(s) 536, 568, 671, 703
AseI ATTAAT 1 cut(s) 129
AspLEI GCGC 1 cut(s) 466
AspS9I GGNCC 1 cut(s) 358
AsuC2I CCSGG 1 cut(s) 962
AsuHPI GGTGA 1 cut(s) 610
AsuII TTCGAA 1 cut(s) 397
AsuNHI GCTAGC 1 cut(s) 931
AvaI CYCGRG 2 cut(s) 263, 321
AvaII GGWCC 1 cut(s) 358
BanII GRGCYC 1 cut(s) 630
Bbv12I GWGCWC 1 cut(s) 630
BbvI GCAGC 5 cut(s) 733, 766, 863, 907, 952
BccI CCATC 2 cut(s) 179, 761
BceAI ACGGC 2 cut(s) 125, 595
BciT130I CCWGG 3 cut(s) 23, 57, 677
BcnI CCSGG 1 cut(s) 962
BcoDI GTCTC 2 cut(s) 556, 618
BfaI CTAG 2 cut(s) 845, 932
BfmI CTRYAG 1 cut(s) 222
BfrI CTTAAG 1 cut(s) 77
BglII AGATCT 1 cut(s) 84
BisI GCNGC 7 cut(s) 100, 747, 780, 877, 880, 896, 941
BlpI GCTNAGC 2 cut(s) 783, 854
BlsI GCNGC 7 cut(s) 101, 748, 781, 878, 881, 897, 942
Bme1390I CCNGG 4 cut(s) 23, 57, 677, 962
Bme18I GGWCC 1 cut(s) 358
BmeT110I CYCGRG 2 cut(s) 263, 321
BmgT120I GGNCC 1 cut(s) 358
BmrFI CCNGG 4 cut(s) 23, 57, 677, 962
BmrI ACTGGG 3 cut(s) 170, 898, 955
BmsI GCATC 2 cut(s) 572, 758
BmtI GCTAGC 1 cut(s) 935
BmuI ACTGGG 3 cut(s) 170, 898, 955
BplI GAGNNNNNCTC 2 cut(s) 569, 601
Bpu1102I GCTNAGC 2 cut(s) 783, 854
Bpu14I TTCGAA 1 cut(s) 397
BpuEI CTTGAG 1 cut(s) 392
BpuMI CCSGG 1 cut(s) 962
BsaI GGTCTC 1 cut(s) 556
BsaJI CCNNGG 4 cut(s) 40, 56, 497, 829
BsaWI WCCGGW 1 cut(s) 116
Bsc4I CCNNNNNNNGG 1 cut(s) 62
Bse118I RCCGGY 1 cut(s) 565
Bse1I ACTGG 4 cut(s) 176, 695, 893, 950
Bse3DI GCAATG 2 cut(s) 169, 387
BseAI TCCGGA 1 cut(s) 116
BseBI CCWGG 3 cut(s) 23, 57, 677
BseDI CCNNGG 4 cut(s) 40, 56, 497, 829
BseGI GGATG 1 cut(s) 587
BseLI CCNNNNNNNGG 1 cut(s) 62
BseMI GCAATG 2 cut(s) 169, 387
BseMII CTCAG 2 cut(s) 774, 880
BseNI ACTGG 4 cut(s) 176, 695, 893, 950
BseXI GCAGC 5 cut(s) 733, 766, 863, 907, 952
Bsh1236I CGCG 1 cut(s) 882
BshFI GGCC 2 cut(s) 39, 449
BsiHKAI GWGCWC 1 cut(s) 630
BsiHKCI CYCGRG 2 cut(s) 263, 321
BsiSI CCGG 3 cut(s) 117, 566, 961
BslI CCNNNNNNNGG 1 cut(s) 62
BsmAI GTCTC 2 cut(s) 556, 618
BsmBI CGTCTC 1 cut(s) 618
BsmI GAATGC 1 cut(s) 965
BsnI GGCC 2 cut(s) 39, 449
Bso31I GGTCTC 1 cut(s) 556
BsoBI CYCGRG 2 cut(s) 263, 321
Bsp119I TTCGAA 1 cut(s) 397
Bsp1286I GDGCHC 1 cut(s) 630
Bsp13I TCCGGA 1 cut(s) 116
Bsp1407I TGTACA 1 cut(s) 838
Bsp143I GATC 4 cut(s) 84, 148, 546, 691
Bsp1720I GCTNAGC 2 cut(s) 783, 854
BspACI CCGC 4 cut(s) 100, 711, 880, 898
BspANI GGCC 2 cut(s) 39, 449
BspCNI CTCAG 2 cut(s) 775, 879
BspEI TCCGGA 1 cut(s) 116
BspFNI CGCG 1 cut(s) 882
BspMAI CTGCAG 1 cut(s) 226
BspOI GCTAGC 1 cut(s) 935
BspPI GGATC 2 cut(s) 143, 686
BspQI GCTCTTC 1 cut(s) 843
BspT104I TTCGAA 1 cut(s) 397
BspTI CTTAAG 1 cut(s) 77
BspTNI GGTCTC 1 cut(s) 556
BsrDI GCAATG 2 cut(s) 169, 387
BsrFI RCCGGY 1 cut(s) 565
BsrGI TGTACA 1 cut(s) 838
BsrI ACTGG 4 cut(s) 176, 695, 893, 950
BssAI RCCGGY 1 cut(s) 565
BssECI CCNNGG 4 cut(s) 40, 56, 497, 829
BssMI GATC 4 cut(s) 84, 148, 546, 691
BssT1I CCWWGG 3 cut(s) 40, 497, 829
Bst2UI CCWGG 3 cut(s) 23, 57, 677
Bst4CI ACNGT 2 cut(s) 685, 957
Bst6I CTCTTC 2 cut(s) 554, 843
BstAFI CTTAAG 1 cut(s) 77
BstAPI GCANNNNNTGC 2 cut(s) 472, 876
BstAUI TGTACA 1 cut(s) 838
BstBI TTCGAA 1 cut(s) 397
BstC8I GCNNGC 4 cut(s) 317, 447, 739, 933
BstDEI CTNAG 3 cut(s) 783, 854, 866
BstF5I GGATG 1 cut(s) 587
BstFNI CGCG 1 cut(s) 882
BstHHI GCGC 1 cut(s) 466
BstKTI GATC 4 cut(s) 87, 151, 549, 694
BstMAI GTCTC 2 cut(s) 556, 618
BstMBI GATC 4 cut(s) 84, 148, 546, 691
BstMWI GCNNNNNNNGC 6 cut(s) 472, 508, 743, 807, 876, 937
BstNI CCWGG 3 cut(s) 23, 57, 677
BstNSI RCATGY 4 cut(s) 34, 162, 301, 666
BstSCI CCNGG 4 cut(s) 21, 55, 675, 960
BstSFI CTRYAG 1 cut(s) 222
BstUI CGCG 1 cut(s) 882
BstV1I GCAGC 5 cut(s) 733, 766, 863, 907, 952
BstX2I RGATCY 1 cut(s) 84
BstYI RGATCY 1 cut(s) 84
BsuRI GGCC 2 cut(s) 39, 449
BtsCI GGATG 1 cut(s) 587
BtsI GCAGTG 1 cut(s) 231
BtsIMutI CAGTG 5 cut(s) 183, 231, 759, 943, 962
Cac8I GCNNGC 4 cut(s) 317, 447, 739, 933
CaiI CAGNNNCTG 1 cut(s) 876
CfoI GCGC 1 cut(s) 466
Cfr10I RCCGGY 1 cut(s) 565
Cfr13I GGNCC 1 cut(s) 358
Csp6I GTAC 5 cut(s) 28, 143, 268, 295, 839
CviAII CATG 8 cut(s) 31, 137, 146, 159, 298, 332, 663, 970
CviQI GTAC 5 cut(s) 28, 143, 268, 295, 839
DdeI CTNAG 3 cut(s) 783, 854, 866
DpnI GATC 4 cut(s) 86, 150, 548, 693
DpnII GATC 4 cut(s) 84, 148, 546, 691
Eam1104I CTCTTC 2 cut(s) 554, 843
EarI CTCTTC 2 cut(s) 554, 843
Ecl136II GAGCTC 1 cut(s) 628
Eco130I CCWWGG 3 cut(s) 40, 497, 829
Eco147I AGGCCT 1 cut(s) 449
Eco24I GRGCYC 1 cut(s) 630
Eco31I GGTCTC 1 cut(s) 556
Eco47I GGWCC 1 cut(s) 358
Eco53kI GAGCTC 1 cut(s) 628
Eco88I CYCGRG 2 cut(s) 263, 321
EcoICRI GAGCTC 1 cut(s) 628
EcoO109I RGGNCCY 1 cut(s) 358
EcoRI GAATTC 1 cut(s) 483
EcoRII CCWGG 3 cut(s) 21, 55, 675
EcoT14I CCWWGG 3 cut(s) 40, 497, 829
EcoT38I GRGCYC 1 cut(s) 630
ErhI CCWWGG 3 cut(s) 40, 497, 829
Esp3I CGTCTC 1 cut(s) 618
FaeI CATG 8 cut(s) 34, 140, 149, 162, 301, 335, 666, 973
FalI AAGNNNNNCTT 2 cut(s) 71, 103
FatI CATG 8 cut(s) 30, 136, 145, 158, 297, 331, 662, 969
Fnu4HI GCNGC 7 cut(s) 100, 747, 780, 877, 880, 896, 941
FokI GGATG 1 cut(s) 594
FriOI GRGCYC 1 cut(s) 630
Fsp4HI GCNGC 7 cut(s) 100, 747, 780, 877, 880, 896, 941
FspBI CTAG 2 cut(s) 845, 932
GlaI GCGC 1 cut(s) 465
GluI GCNGC 7 cut(s) 100, 747, 780, 877, 880, 896, 941
HaeIII GGCC 2 cut(s) 39, 449
HapII CCGG 3 cut(s) 117, 566, 961
HhaI GCGC 1 cut(s) 466
Hin1II CATG 8 cut(s) 34, 140, 149, 162, 301, 335, 666, 973
Hin6I GCGC 1 cut(s) 464
HinP1I GCGC 1 cut(s) 464
HindIII AAGCTT 1 cut(s) 74
HinfI GANTC 5 cut(s) 394, 424, 635, 901, 950
HpaII CCGG 3 cut(s) 117, 566, 961
HphI GGTGA 1 cut(s) 610
Hpy166II GTNNAC 3 cut(s) 757, 826, 841
Hpy188I TCNGA 1 cut(s) 906
Hpy188III TCNNGA 4 cut(s) 70, 88, 117, 409
Hpy8I GTNNAC 3 cut(s) 757, 826, 841
HpyAV CCTTC 3 cut(s) 210, 241, 344
HpyCH4III ACNGT 2 cut(s) 685, 957
HpyCH4V TGCA 5 cut(s) 12, 34, 224, 475, 749
HpyF10VI GCNNNNNNNGC 6 cut(s) 472, 508, 743, 807, 876, 937
HpyF3I CTNAG 3 cut(s) 783, 854, 866
Hsp92II CATG 8 cut(s) 34, 140, 149, 162, 301, 335, 666, 973
HspAI GCGC 1 cut(s) 464
Kpn2I TCCGGA 1 cut(s) 116
Kzo9I GATC 4 cut(s) 84, 148, 546, 691
LguI GCTCTTC 1 cut(s) 843
LmnI GCTCC 1 cut(s) 892
Lsp1109I GCAGC 5 cut(s) 733, 766, 863, 907, 952
LweI GCATC 2 cut(s) 572, 758
MaeI CTAG 2 cut(s) 845, 932
MaeIII GTNAC 2 cut(s) 364, 456
MalI GATC 4 cut(s) 86, 150, 548, 693
MboI GATC 4 cut(s) 84, 148, 546, 691
MboII GAAGA 7 cut(s) 58, 94, 103, 479, 556, 571, 860
MflI RGATCY 1 cut(s) 84
MhlI GDGCHC 1 cut(s) 630
MluCI AATT 3 cut(s) 257, 483, 529
MnlI CCTC 5 cut(s) 317, 330, 571, 808, 915
MroI TCCGGA 1 cut(s) 116
MseI TTAA 8 cut(s) 78, 129, 240, 341, 381, 453, 533, 773
MslI CAYNNNNRTG 1 cut(s) 302
MspA1I CMGCKG 1 cut(s) 898
MspCI CTTAAG 1 cut(s) 77
MspI CCGG 3 cut(s) 117, 566, 961
MspR9I CCNGG 4 cut(s) 23, 57, 677, 962
Mva1269I GAATGC 1 cut(s) 965
MvaI CCWGG 3 cut(s) 23, 57, 677
MvnI CGCG 1 cut(s) 882
MwoI GCNNNNNNNGC 6 cut(s) 472, 508, 743, 807, 876, 937
NciI CCSGG 1 cut(s) 962
NdeII GATC 4 cut(s) 84, 148, 546, 691
NheI GCTAGC 1 cut(s) 931
NlaIII CATG 8 cut(s) 34, 140, 149, 162, 301, 335, 666, 973
NmeAIII GCCGAG 2 cut(s) 722, 836
NmuCI GTSAC 2 cut(s) 364, 456
NspI RCATGY 4 cut(s) 34, 162, 301, 666
NspV TTCGAA 1 cut(s) 397
PaeR7I CTCGAG 2 cut(s) 263, 321
PceI AGGCCT 1 cut(s) 449
PciI ACATGT 1 cut(s) 662
PciSI GCTCTTC 1 cut(s) 843
PctI GAATGC 1 cut(s) 965
PfeI GAWTC 5 cut(s) 394, 424, 635, 901, 950
PflFI GACNNNGTC 1 cut(s) 362
PkrI GCNGC 7 cut(s) 101, 748, 781, 878, 881, 897, 942
PpuMI RGGWCCY 1 cut(s) 358
PscI ACATGT 1 cut(s) 662
PshBI ATTAAT 1 cut(s) 129
Psp124BI GAGCTC 1 cut(s) 630
Psp5II RGGWCCY 1 cut(s) 358
Psp6I CCWGG 3 cut(s) 21, 55, 675
PspGI CCWGG 3 cut(s) 21, 55, 675
PspPI GGNCC 1 cut(s) 358
PspPPI RGGWCCY 1 cut(s) 358
PspXI VCTCGAGB 2 cut(s) 263, 321
PstI CTGCAG 1 cut(s) 226
PstNI CAGNNNCTG 1 cut(s) 876
PsuI RGATCY 1 cut(s) 84
PsyI GACNNNGTC 1 cut(s) 362
RsaI GTAC 5 cut(s) 29, 144, 269, 296, 840
RsaNI GTAC 5 cut(s) 28, 143, 268, 295, 839
RseI CAYNNNNRTG 1 cut(s) 302
SacI GAGCTC 1 cut(s) 630
SapI GCTCTTC 1 cut(s) 843
SaqAI TTAA 8 cut(s) 78, 129, 240, 341, 381, 453, 533, 773
SatI GCNGC 7 cut(s) 100, 747, 780, 877, 880, 896, 941
Sau3AI GATC 4 cut(s) 84, 148, 546, 691
Sau96I GGNCC 1 cut(s) 358
ScrFI CCNGG 4 cut(s) 23, 57, 677, 962
SduI GDGCHC 1 cut(s) 630
SfaNI GCATC 2 cut(s) 572, 758
SfcI CTRYAG 1 cut(s) 222
Sfr274I CTCGAG 2 cut(s) 263, 321
SfuI TTCGAA 1 cut(s) 397
SinI GGWCC 1 cut(s) 358
SlaI CTCGAG 2 cut(s) 263, 321
SmiMI CAYNNNNRTG 1 cut(s) 302
SmlI CTYRAG 4 cut(s) 77, 263, 321, 407
SmoI CTYRAG 4 cut(s) 77, 263, 321, 407
Sse9I AATT 3 cut(s) 257, 483, 529
SseBI AGGCCT 1 cut(s) 449
SsiI CCGC 4 cut(s) 100, 711, 880, 898
SspMI CTAG 2 cut(s) 845, 932
SstI GAGCTC 1 cut(s) 630
StuI AGGCCT 1 cut(s) 449
StyD4I CCNGG 4 cut(s) 21, 55, 675, 960
StyI CCWWGG 3 cut(s) 40, 497, 829
TaaI ACNGT 2 cut(s) 685, 957
TaqI TCGA 5 cut(s) 264, 322, 397, 542, 765
TasI AATT 3 cut(s) 257, 483, 529
TatI WGTACW 3 cut(s) 27, 294, 838
TauI GCSGC 2 cut(s) 102, 882
TfiI GAWTC 5 cut(s) 394, 424, 635, 901, 950
Tru1I TTAA 8 cut(s) 78, 129, 240, 341, 381, 453, 533, 773
Tru9I TTAA 8 cut(s) 78, 129, 240, 341, 381, 453, 533, 773
TscAI CASTG 5 cut(s) 183, 231, 759, 950, 962
TseFI GTSAC 2 cut(s) 364, 456
TseI GCWGC 5 cut(s) 746, 779, 876, 895, 940
Tsp45I GTSAC 2 cut(s) 364, 456
TspDTI ATGAA 5 cut(s) 17, 228, 416, 611, 958
TspRI CASTG 5 cut(s) 183, 231, 759, 950, 962
Tth111I GACNNNGTC 1 cut(s) 362
Vha464I CTTAAG 1 cut(s) 77
VpaK11BI GGWCC 1 cut(s) 358
VspI ATTAAT 1 cut(s) 129
XapI RAATTY 1 cut(s) 483
XceI RCATGY 4 cut(s) 34, 162, 301, 666
XcmI CCANNNNNNNNNTGG 1 cut(s) 278
XhoI CTCGAG 2 cut(s) 263, 321
XspI CTAG 2 cut(s) 845, 932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.