AT2G43760

Catalytic subunit of the molybdopterin synthase complex, a complex that catalyzes the conversion of precursor Z into molybdopterin. Acts by mediating the incorporation of 2 sulfur atoms from thiocarboxylated MOCS2A into precursor Z to generate a dithiolene group

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0015233
Physical Location & Seq
Forward (+)
18132484 .. 18134068
1585 bp
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UTR
Exon/CDS
Intron
AT2G43760.1

Sequence Viewer

Length: 597 bp
ATGTCTGCAGAGGAGAAGAACCTTATAGAGATCTTGGAGGAAGGTCACAAAGTCGATGTTGTTAAGTACATTGATTATGTCAGTGCCCCTCAAGCTGGTGCTATTGCGACATTCTCAGGCACTACAAGAGACATGTTCGAGGGCAAGACAGTGTTGGAGTTAAGGTATGAAGCATATGTGCCAATGGCCACACGATGCCTCAGCTCGATCTGTACAACTGCTAGATCAACTTGGGATATCCACAAGATTGCTGTTGCTCACCGTCTTGGACCAGTTCCTGTTGGAGAAACTAGCGTGCTCATTGCGGTCTCATCTGTTCACCGTGCAGATGGTTTAGATGCTTGCAAGTTCTTGATCGACGAGTTGAAGGCATCGGTTCCGATATGGAAGAAAGAGGTTTACACTAATGGAGAGATTTGGAAGGAGAATTCTGAGTTTATGGAGAAAAGATTGGAGCTTGCAGAGAAAAGAGACAGCATTGTAAAAAAAACTGTGGTTGAAGAACATAGAAGAAGAGGTTGCTGTGGAAGCAAAGTTAGAGTAGAAGAAGATGAAGAGCATAAAGATATTACAGGAGATAACAAATCTTCTTCTTGA

Protein Analysis

198

Amino Acids

22.22

Weight (kDa)

5.76

Isoelectric Point (pI)

50.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MoaE PF02391 17 - 124 7.6e-38 MoaE protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015233)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43760 AT2G43760 AT2G43760
fragaria_vesca FvH4_1g18980
prunus_persica Prupe.4G269700_v2.0.a1 Prupe.4G269700_v2.0.a1 Prupe.4G269700_v2.0.a1
pyrus_communis pycom5153684g00150
rosa_chinensis RchiOBHm_Chr2g0110001
rosa_roxburghii Rroxscaffold_2G00133790
rosa_rugosa Rorug02G0165100
rosa_samantha Rh2AG216600 Rh2BG226600 Rh2CG218800 Rh2DG222200
rosa_wichuraiana Rw2G016740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 305
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 1 cut(s) 427
AfaI GTAC 2 cut(s) 68, 214
AfiI CCNNNNNNNGG 1 cut(s) 95
AflIII ACRYGT 1 cut(s) 132
AgsI TTSAA 2 cut(s) 367, 500
AluBI AGCT 3 cut(s) 95, 204, 457
AluI AGCT 3 cut(s) 95, 204, 457
Alw21I GWGCWC 1 cut(s) 300
Alw26I GTCTC 3 cut(s) 123, 313, 465
AlwNI CAGNNNCTG 1 cut(s) 278
AoxI GGCC 1 cut(s) 186
ApoI RAATTY 1 cut(s) 427
AspS9I GGNCC 1 cut(s) 269
AsuHPI GGTGA 2 cut(s) 251, 311
AvaII GGWCC 1 cut(s) 269
BaeGI GKGCMC 1 cut(s) 88
BalI TGGCCA 1 cut(s) 188
Bbv12I GWGCWC 1 cut(s) 300
BbvCI CCTCAGC 1 cut(s) 200
BccI CCATC 1 cut(s) 323
BcoDI GTCTC 3 cut(s) 123, 313, 465
BfaI CTAG 2 cut(s) 222, 291
BfmI CTRYAG 1 cut(s) 6
BglII AGATCT 1 cut(s) 30
Bme18I GGWCC 1 cut(s) 269
BmgT120I GGNCC 1 cut(s) 269
BmiI GGNNCC 1 cut(s) 378
BmsI GCATC 3 cut(s) 185, 328, 380
Bpu10I CCTNAGC 1 cut(s) 200
BpuEI CTTGAG 1 cut(s) 75
BsaI GGTCTC 1 cut(s) 313
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 1 cut(s) 95
Bse1I ACTGG 1 cut(s) 272
Bse3DI GCAATG 1 cut(s) 300
BseLI CCNNNNNNNGG 1 cut(s) 95
BseMI GCAATG 1 cut(s) 300
BseMII CTCAG 3 cut(s) 129, 214, 423
BseNI ACTGG 1 cut(s) 272
BseRI GAGGAG 1 cut(s) 26
BseSI GKGCMC 1 cut(s) 88
BsgI GTGCAG 1 cut(s) 345
BshFI GGCC 1 cut(s) 188
BsiHKAI GWGCWC 1 cut(s) 300
BslI CCNNNNNNNGG 1 cut(s) 95
BsmAI GTCTC 3 cut(s) 123, 313, 465
BsnI GGCC 1 cut(s) 188
Bso31I GGTCTC 1 cut(s) 313
Bsp1286I GDGCHC 2 cut(s) 88, 300
Bsp1407I TGTACA 1 cut(s) 212
Bsp143I GATC 4 cut(s) 30, 207, 224, 354
BspACI CCGC 1 cut(s) 305
BspANI GGCC 1 cut(s) 188
BspCNI CTCAG 3 cut(s) 128, 213, 424
BspLI GGNNCC 1 cut(s) 378
BspMAI CTGCAG 1 cut(s) 10
BspQI GCTCTTC 1 cut(s) 549
BspTNI GGTCTC 1 cut(s) 313
BsrDI GCAATG 1 cut(s) 300
BsrGI TGTACA 1 cut(s) 212
BsrI ACTGG 1 cut(s) 272
BssMI GATC 4 cut(s) 30, 207, 224, 354
Bst4CI ACNGT 4 cut(s) 151, 263, 323, 493
Bst6I CTCTTC 2 cut(s) 508, 549
BstAUI TGTACA 1 cut(s) 212
BstC8I GCNNGC 3 cut(s) 296, 343, 459
BstDEI CTNAG 3 cut(s) 115, 200, 432
BstKTI GATC 4 cut(s) 33, 210, 227, 357
BstMAI GTCTC 3 cut(s) 123, 313, 465
BstMBI GATC 4 cut(s) 30, 207, 224, 354
BstMWI GCNNNNNNNGC 2 cut(s) 92, 528
BstNSI RCATGY 1 cut(s) 136
BstSFI CTRYAG 1 cut(s) 6
BstSLI GKGCMC 1 cut(s) 88
BstX2I RGATCY 1 cut(s) 30
BstYI RGATCY 1 cut(s) 30
BsuRI GGCC 1 cut(s) 188
BtsIMutI CAGTG 2 cut(s) 88, 156
Cac8I GCNNGC 3 cut(s) 296, 343, 459
CaiI CAGNNNCTG 1 cut(s) 278
Cfr13I GGNCC 1 cut(s) 269
Csp6I GTAC 2 cut(s) 67, 213
CviAII CATG 1 cut(s) 133
CviJI RGCY 4 cut(s) 95, 188, 204, 457
CviKI_1 RGCY 4 cut(s) 95, 188, 204, 457
CviQI GTAC 2 cut(s) 67, 213
DdeI CTNAG 3 cut(s) 115, 200, 432
DpnI GATC 4 cut(s) 32, 209, 226, 356
DpnII GATC 4 cut(s) 30, 207, 224, 354
EaeI YGGCCR 1 cut(s) 186
Eam1104I CTCTTC 2 cut(s) 508, 549
EarI CTCTTC 2 cut(s) 508, 549
Eco31I GGTCTC 1 cut(s) 313
Eco32I GATATC 1 cut(s) 238
Eco47I GGWCC 1 cut(s) 269
EcoRI GAATTC 1 cut(s) 427
EcoRV GATATC 1 cut(s) 238
FaeI CATG 1 cut(s) 136
FatI CATG 1 cut(s) 132
FauNDI CATATG 1 cut(s) 175
FspBI CTAG 2 cut(s) 222, 291
HaeIII GGCC 1 cut(s) 188
Hin1II CATG 1 cut(s) 136
HphI GGTGA 2 cut(s) 251, 311
Hpy166II GTNNAC 2 cut(s) 319, 400
Hpy188I TCNGA 2 cut(s) 381, 433
Hpy188III TCNNGA 2 cut(s) 352, 594
Hpy8I GTNNAC 2 cut(s) 319, 400
Hpy99I CGWCG 1 cut(s) 362
HpyAV CCTTC 3 cut(s) 35, 361, 415
HpyCH4III ACNGT 4 cut(s) 151, 263, 323, 493
HpyCH4V TGCA 4 cut(s) 8, 326, 345, 461
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 528
HpyF3I CTNAG 3 cut(s) 115, 200, 432
Hsp92II CATG 1 cut(s) 136
Kzo9I GATC 4 cut(s) 30, 207, 224, 354
LguI GCTCTTC 1 cut(s) 549
LmnI GCTCC 1 cut(s) 454
LpnPI CCDG 5 cut(s) 81, 102, 285, 291, 558
LweI GCATC 3 cut(s) 185, 328, 380
MaeI CTAG 2 cut(s) 222, 291
MaeIII GTNAC 1 cut(s) 44
MalI GATC 4 cut(s) 32, 209, 226, 356
MboI GATC 4 cut(s) 30, 207, 224, 354
MflI RGATCY 1 cut(s) 30
MhlI GDGCHC 2 cut(s) 88, 300
MlsI TGGCCA 1 cut(s) 188
MluCI AATT 1 cut(s) 427
MluNI TGGCCA 1 cut(s) 188
MmeI TCCRAC 2 cut(s) 135, 262
MnlI CCTC 7 cut(s) 4, 31, 99, 133, 209, 388, 509
Mox20I TGGCCA 1 cut(s) 188
MscI TGGCCA 1 cut(s) 188
MseI TTAA 2 cut(s) 63, 161
Msp20I TGGCCA 1 cut(s) 188
MwoI GCNNNNNNNGC 2 cut(s) 92, 528
NdeI CATATG 1 cut(s) 175
NdeII GATC 4 cut(s) 30, 207, 224, 354
NlaIII CATG 1 cut(s) 136
NlaIV GGNNCC 1 cut(s) 378
NmuCI GTSAC 1 cut(s) 44
NspI RCATGY 1 cut(s) 136
PciI ACATGT 1 cut(s) 132
PciSI GCTCTTC 1 cut(s) 549
PscI ACATGT 1 cut(s) 132
PspN4I GGNNCC 1 cut(s) 378
PspPI GGNCC 1 cut(s) 269
PstI CTGCAG 1 cut(s) 10
PstNI CAGNNNCTG 1 cut(s) 278
PsuI RGATCY 1 cut(s) 30
RsaI GTAC 2 cut(s) 68, 214
RsaNI GTAC 2 cut(s) 67, 213
SapI GCTCTTC 1 cut(s) 549
SaqAI TTAA 2 cut(s) 63, 161
Sau3AI GATC 4 cut(s) 30, 207, 224, 354
Sau96I GGNCC 1 cut(s) 269
SduI GDGCHC 2 cut(s) 88, 300
SetI ASST 8 cut(s) 24, 46, 97, 167, 206, 399, 459, 520
SfaNI GCATC 3 cut(s) 185, 328, 380
SfcI CTRYAG 1 cut(s) 6
SinI GGWCC 1 cut(s) 269
SmlI CTYRAG 1 cut(s) 90
SmoI CTYRAG 1 cut(s) 90
Sse9I AATT 1 cut(s) 427
SsiI CCGC 1 cut(s) 305
SspMI CTAG 2 cut(s) 222, 291
TaaI ACNGT 4 cut(s) 151, 263, 323, 493
TaqI TCGA 4 cut(s) 54, 138, 206, 357
TasI AATT 1 cut(s) 427
TatI WGTACW 2 cut(s) 66, 212
Tru1I TTAA 2 cut(s) 63, 161
Tru9I TTAA 2 cut(s) 63, 161
TscAI CASTG 2 cut(s) 88, 156
TseFI GTSAC 1 cut(s) 44
Tsp45I GTSAC 1 cut(s) 44
TspDTI ATGAA 2 cut(s) 183, 567
TspRI CASTG 2 cut(s) 88, 156
VpaK11BI GGWCC 1 cut(s) 269
XapI RAATTY 1 cut(s) 427
XceI RCATGY 1 cut(s) 136
XspI CTAG 2 cut(s) 222, 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.