AT2G47970

npl4-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0013136
Physical Location & Seq
Forward (+)
19629217 .. 19631188
1972 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G47970.2

Sequence Viewer

Length: 1242 bp
ATGATGATGCTCAGAATCCGAAGCAGAGATGGACTGGAGAGAGTCACAGCGGAGGGAGCTCATATCACTGTTTCCCAGCTCAAAACCTTGATCGCGGATCAGCTTCAGATCCCTCTCCATAAGCAGACCTTATCCACCAATCGTGATCTCCTCCTCGCCAAAACTCCCGCTGATCTACTCGCCTTCACCGACCTCACGGACCCAAACCTCCCCCTCTCCTCTCTCAACCTCGGCCATGGCTCTATGCTCTACCTCGCCTACGATGGCGAGCGCTCCATTCCTGGAGCTCCTCCCGTTACTCCTGCCGGATCCTTCGGGAGGAAGATGACCGTCGATGACTTGATCGCCCGCCAGATGCGCGTCACTCGTCAGGAGACGTCTCACTGCGACTCCGTCTCCTTCGATCGCGATGCCGCCAACGCTTTCCAGCACTACGTCAATGAGTCTCTAGCTTTTGCCGTCAAGCGCGGCGGCTTTATGTACGGCACCGTCACCGAGGAAGGACAGGTGGAAGTTGATTTCATTTACGAGCCGCCCCAGCAAGGCACGGAGGCTAATCTTATTCTGATGAGAGATGCTGATGAAGAGAAGCGCGTGGACGCAATCGCCATGGGCTTGGGGATGAGGAGGGTCGGTTTCATTTTTAACCAGACCGTGGTGCAGGACAAGACTGAATACACTCTGTCCAACGCCGAGGTGCTACAGGCGGCGGAGCTTCACGCCGAGAGCGAGCTGAAGGAGTGGGTCACGGCGGTGGTGAAGCTTGAGGTGAATGAGGATGGTGGCGCCGATGTCCATTTCGAGGCGTTTCAAATGAGTGATATGTGCATCAGGCTGTTCAAGGAAGAGTGGTTCGAGACGGAGATTATGCCGGACGATGATCCAAAGCTCTCTAAGATGAAGAAGGAGGTGGTTGTTGGTGTGAAGGATCTTAAGGAGGTAGATAACGATTTCTTCCTCGTTCTCGTCAGGATCTTGGATCACCAGGGGCCTCTGTCATCAACATTCCCAATAGAGAACCGGAGCAGTCGAGCAACGATGCGGGCTCTAAAAACACATCTGGACCGGGCAAAGAGCTTGCCGTTGGTGAAGAAGATGTCAGATTTTCATCTGCTTCTCTTTGTGGCTCAGTTTCTGGACGTGTCATCGGACGTCCCTGCTTTAGCCGAGTGTGTGCGTCTGCAGTCGCCTGTCCCAGAGGGCTATGCGTTGCTCATCGAATCAATGGCCAACACTTGTTAA

Protein Analysis

413

Amino Acids

46.17

Weight (kDa)

5.0

Isoelectric Point (pI)

45.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UN_NPL4 PF11543 2 - 85 1e-06 Nuclear pore localisation protein NPL4
NPL4 PF05021 158 - 291 1e-11 NPL4 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013136)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47970 AT3G63000
fragaria_vesca FvH4_2g24580
malus_domestica MD03G1274300.v1.1
prunus_persica Prupe.8G251000_v2.0.a1
pyrus_communis pycom03g21870 pycom11g26110
rosa_chinensis RchiOBHm_Chr6g0292401
rosa_laevigata RLG00000012031
rosa_multiflora Rmu_sc0001354.1_g000016 Rmu_sc0012226.1_g000002
rosa_roxburghii Rroxscaffold_7G00175090
rosa_rugosa Rorug06G0226400
rosa_samantha Rh6AG339700 Rh6BG346900 Rh6CG353700 Rh6DG339700
rosa_wichuraiana Rw6G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 380, 1155
AccB1I GGYRCC 2 cut(s) 485, 785
AccB7I CCANNNNNTGG 1 cut(s) 655
AccII CGCG 5 cut(s) 95, 360, 408, 468, 594
AclWI GGATC 8 cut(s) 103, 105, 303, 316, 875, 936, 980, 987
AcoI YGGCCR 2 cut(s) 232, 1227
AcuI CTGAAG 2 cut(s) 89, 755
AcyI GRCGYC 3 cut(s) 377, 786, 1152
AfaI GTAC 1 cut(s) 482
AfeI AGCGCT 1 cut(s) 272
AfiI CCNNNNNNNGG 4 cut(s) 318, 542, 655, 802
AflII CTTAAG 1 cut(s) 932
AflIII ACRYGT 1 cut(s) 1140
AgsI TTSAA 2 cut(s) 812, 841
AjiI CACGTC 1 cut(s) 1141
AjnI CCWGG 2 cut(s) 280, 984
AleI CACNNNNGTG 1 cut(s) 752
AloI GAACNNNNNNTCC 2 cut(s) 836, 868
Alw21I GWGCWC 2 cut(s) 61, 289
Alw26I GTCTC 5 cut(s) 368, 384, 400, 450, 851
AlwI GGATC 8 cut(s) 103, 105, 303, 316, 875, 936, 980, 987
AlwNI CAGNNNCTG 1 cut(s) 1135
Aor51HI AGCGCT 1 cut(s) 272
AoxI GGCC 3 cut(s) 232, 989, 1227
AspLEI GCGC 5 cut(s) 273, 360, 468, 594, 788
AspS9I GGNCC 3 cut(s) 199, 989, 1063
AsuC2I CCSGG 1 cut(s) 1067
AsuHPI GGTGA 6 cut(s) 178, 484, 769, 781, 974, 1099
AvaII GGWCC 2 cut(s) 199, 1063
BalI TGGCCA 1 cut(s) 1229
BamHI GGATCC 1 cut(s) 308
BanI GGYRCC 2 cut(s) 485, 785
BanII GRGCYC 3 cut(s) 61, 289, 1048
Bbv12I GWGCWC 2 cut(s) 61, 289
BccI CCATC 3 cut(s) 23, 257, 773
BceAI ACGGC 4 cut(s) 443, 499, 765, 1066
BcgI CGANNNNNNTGC 2 cut(s) 392, 426
BciT130I CCWGG 2 cut(s) 282, 986
BcnI CCSGG 1 cut(s) 1067
BcoDI GTCTC 5 cut(s) 368, 384, 400, 450, 851
BfaI CTAG 1 cut(s) 449
BfmI CTRYAG 2 cut(s) 701, 1181
BfoI RGCGCY 2 cut(s) 274, 789
BfrI CTTAAG 1 cut(s) 932
BisI GCNGC 5 cut(s) 414, 469, 472, 533, 708
BlsI GCNGC 5 cut(s) 415, 470, 473, 534, 709
Bme1390I CCNGG 3 cut(s) 282, 986, 1067
Bme18I GGWCC 2 cut(s) 199, 1063
BmgBI CACGTC 1 cut(s) 1141
BmgT120I GGNCC 3 cut(s) 199, 989, 1063
BmiI GGNNCC 5 cut(s) 201, 310, 487, 787, 990
BmrFI CCNGG 3 cut(s) 282, 986, 1067
BmsI GCATC 5 cut(s) 345, 400, 565, 837, 1029
BpmI CTGGAG 2 cut(s) 56, 303
BpuEI CTTGAG 1 cut(s) 785
BpuMI CCSGG 1 cut(s) 1067
BsaBI GATNNNNATC 1 cut(s) 1107
BsaHI GRCGYC 3 cut(s) 377, 786, 1152
BsaJI CCNNGG 7 cut(s) 229, 235, 495, 609, 654, 693, 985
BsaWI WCCGGW 1 cut(s) 1020
BsaXI ACNNNNNCTCC 6 cut(s) 374, 380, 404, 410, 619, 649
Bsc4I CCNNNNNNNGG 4 cut(s) 318, 542, 655, 802
Bse1I ACTGG 1 cut(s) 39
Bse8I GATNNNNATC 1 cut(s) 1107
BseBI CCWGG 2 cut(s) 282, 986
BseDI CCNNGG 7 cut(s) 229, 235, 495, 609, 654, 693, 985
BseGI GGATG 2 cut(s) 627, 784
BseJI GATNNNNATC 1 cut(s) 1107
BseLI CCNNNNNNNGG 4 cut(s) 318, 542, 655, 802
BseMII CTCAG 2 cut(s) 25, 1142
BseNI ACTGG 1 cut(s) 39
BseRI GAGGAG 5 cut(s) 140, 143, 208, 279, 640
BseYI CCCAGC 2 cut(s) 75, 537
BsgI GTGCAG 1 cut(s) 680
Bsh1236I CGCG 5 cut(s) 95, 360, 408, 468, 594
Bsh1285I CGRYCG 1 cut(s) 406
BshFI GGCC 3 cut(s) 234, 991, 1229
BshNI GGYRCC 2 cut(s) 485, 785
BsiEI CGRYCG 1 cut(s) 406
BsiHKAI GWGCWC 2 cut(s) 61, 289
BsiSI CCGG 4 cut(s) 306, 872, 1021, 1066
BslFI GGGAC 2 cut(s) 1139, 1178
BslI CCNNNNNNNGG 4 cut(s) 318, 542, 655, 802
BsmAI GTCTC 5 cut(s) 368, 384, 400, 450, 851
BsmBI CGTCTC 4 cut(s) 368, 384, 400, 851
BsmFI GGGAC 2 cut(s) 1139, 1178
BsnI GGCC 3 cut(s) 234, 991, 1229
Bsp1286I GDGCHC 3 cut(s) 61, 289, 1048
Bsp19I CCATGG 2 cut(s) 235, 609
Bsp68I TCGCGA 1 cut(s) 408
BspANI GGCC 3 cut(s) 234, 991, 1229
BspCNI CTCAG 2 cut(s) 24, 1141
BspFNI CGCG 5 cut(s) 95, 360, 408, 468, 594
BspLI GGNNCC 5 cut(s) 201, 310, 487, 787, 990
BspMAI CTGCAG 1 cut(s) 1185
BspPI GGATC 8 cut(s) 103, 105, 303, 316, 875, 936, 980, 987
BspT107I GGYRCC 2 cut(s) 485, 785
BspTI CTTAAG 1 cut(s) 932
BsrI ACTGG 1 cut(s) 39
BssECI CCNNGG 7 cut(s) 229, 235, 495, 609, 654, 693, 985
BssNI GRCGYC 3 cut(s) 377, 786, 1152
BssT1I CCWWGG 2 cut(s) 235, 609
Bst2UI CCWGG 2 cut(s) 282, 986
Bst4CI ACNGT 4 cut(s) 70, 331, 490, 655
Bst6I CTCTTC 2 cut(s) 579, 840
BstACI GRCGYC 3 cut(s) 377, 786, 1152
BstAFI CTTAAG 1 cut(s) 932
BstC8I GCNNGC 5 cut(s) 269, 349, 731, 1044, 1079
BstDEI CTNAG 3 cut(s) 11, 894, 1128
BstDSI CCRYGG 3 cut(s) 235, 609, 654
BstENI CCTNNNNNAGG 1 cut(s) 316
BstF5I GGATG 2 cut(s) 627, 784
BstFNI CGCG 5 cut(s) 95, 360, 408, 468, 594
BstH2I RGCGCY 2 cut(s) 274, 789
BstHHI GCGC 5 cut(s) 273, 360, 468, 594, 788
BstMAI GTCTC 5 cut(s) 368, 384, 400, 450, 851
BstMCI CGRYCG 1 cut(s) 406
BstMWI GCNNNNNNNGC 4 cut(s) 56, 357, 419, 538
BstNI CCWGG 2 cut(s) 282, 986
BstSCI CCNGG 3 cut(s) 280, 984, 1065
BstSFI CTRYAG 2 cut(s) 701, 1181
BstUI CGCG 5 cut(s) 95, 360, 408, 468, 594
BstX2I RGATCY 4 cut(s) 108, 308, 928, 972
BstXI CCANNNNNNTGG 1 cut(s) 616
BstYI RGATCY 4 cut(s) 108, 308, 928, 972
BsuRI GGCC 3 cut(s) 234, 991, 1229
BtgI CCRYGG 3 cut(s) 235, 609, 654
BtgZI GCGATG 1 cut(s) 423
BtrI CACGTC 1 cut(s) 1141
BtsCI GGATG 2 cut(s) 627, 784
BtsI GCAGTG 1 cut(s) 382
BtsIMutI CAGTG 2 cut(s) 66, 382
BtuMI TCGCGA 1 cut(s) 408
Cac8I GCNNGC 5 cut(s) 269, 349, 731, 1044, 1079
CaiI CAGNNNCTG 1 cut(s) 1135
CfoI GCGC 5 cut(s) 273, 360, 468, 594, 788
Cfr13I GGNCC 3 cut(s) 199, 989, 1063
CseI GACGC 3 cut(s) 349, 608, 1166
Csp6I GTAC 1 cut(s) 481
CviAII CATG 2 cut(s) 236, 610
CviQI GTAC 1 cut(s) 481
DdeI CTNAG 3 cut(s) 11, 894, 1128
DinI GGCGCC 1 cut(s) 787
EaeI YGGCCR 2 cut(s) 232, 1227
Eam1104I CTCTTC 2 cut(s) 579, 840
EarI CTCTTC 2 cut(s) 579, 840
EciI GGCGGA 1 cut(s) 725
Ecl136II GAGCTC 2 cut(s) 59, 287
Eco130I CCWWGG 2 cut(s) 235, 609
Eco24I GRGCYC 3 cut(s) 61, 289, 1048
Eco47I GGWCC 2 cut(s) 199, 1063
Eco47III AGCGCT 1 cut(s) 272
Eco53kI GAGCTC 2 cut(s) 59, 287
Eco57I CTGAAG 2 cut(s) 89, 755
EcoICRI GAGCTC 2 cut(s) 59, 287
EcoNI CCTNNNNNAGG 1 cut(s) 316
EcoO109I RGGNCCY 1 cut(s) 989
EcoRII CCWGG 2 cut(s) 280, 984
EcoT14I CCWWGG 2 cut(s) 235, 609
EcoT38I GRGCYC 3 cut(s) 61, 289, 1048
EgeI GGCGCC 1 cut(s) 787
EheI GGCGCC 1 cut(s) 787
ErhI CCWWGG 2 cut(s) 235, 609
Esp3I CGTCTC 4 cut(s) 368, 384, 400, 851
FaeI CATG 2 cut(s) 239, 613
FaiI YATR 9 cut(s) 63, 120, 237, 245, 479, 611, 824, 869, 1206
FalI AAGNNNNNCTT 2 cut(s) 113, 145
FaqI GGGAC 2 cut(s) 1139, 1178
FatI CATG 2 cut(s) 235, 609
FauI CCCGC 3 cut(s) 175, 356, 1035
Fnu4HI GCNGC 5 cut(s) 414, 469, 472, 533, 708
FokI GGATG 2 cut(s) 634, 791
FriOI GRGCYC 3 cut(s) 61, 289, 1048
Fsp4HI GCNGC 5 cut(s) 414, 469, 472, 533, 708
FspBI CTAG 1 cut(s) 449
GlaI GCGC 5 cut(s) 272, 359, 467, 593, 787
GluI GCNGC 5 cut(s) 414, 469, 472, 533, 708
GsaI CCCAGC 2 cut(s) 79, 541
GsuI CTGGAG 2 cut(s) 56, 303
HaeII RGCGCY 2 cut(s) 274, 789
HaeIII GGCC 3 cut(s) 234, 991, 1229
HapII CCGG 4 cut(s) 306, 872, 1021, 1066
HgaI GACGC 3 cut(s) 349, 608, 1166
HhaI GCGC 5 cut(s) 273, 360, 468, 594, 788
Hin1I GRCGYC 3 cut(s) 377, 786, 1152
Hin1II CATG 2 cut(s) 239, 613
Hin6I GCGC 5 cut(s) 271, 358, 466, 592, 786
HinP1I GCGC 5 cut(s) 271, 358, 466, 592, 786
HindIII AAGCTT 1 cut(s) 761
HinfI GANTC 5 cut(s) 15, 42, 389, 443, 1220
HpaII CCGG 4 cut(s) 306, 872, 1021, 1066
HphI GGTGA 6 cut(s) 178, 484, 769, 781, 974, 1099
Hpy166II GTNNAC 1 cut(s) 598
Hpy188I TCNGA 6 cut(s) 14, 20, 108, 567, 1102, 1150
Hpy188III TCNNGA 8 cut(s) 143, 316, 371, 407, 856, 970, 1061, 1136
Hpy8I GTNNAC 1 cut(s) 598
Hpy99I CGWCG 1 cut(s) 335
HpyAV CCTTC 7 cut(s) 193, 322, 409, 494, 730, 898, 919
HpyCH4III ACNGT 4 cut(s) 70, 331, 490, 655
HpyCH4IV ACGT 4 cut(s) 377, 435, 1140, 1152
HpyCH4V TGCA 3 cut(s) 661, 828, 1183
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 357, 419, 538
HpyF3I CTNAG 3 cut(s) 11, 894, 1128
HpySE526I ACGT 4 cut(s) 377, 435, 1140, 1152
Hsp92I GRCGYC 3 cut(s) 377, 786, 1152
Hsp92II CATG 2 cut(s) 239, 613
HspAI GCGC 5 cut(s) 271, 358, 466, 592, 786
KasI GGCGCC 1 cut(s) 785
LmnI GCTCC 6 cut(s) 56, 278, 284, 292, 712, 1023
LweI GCATC 5 cut(s) 345, 400, 565, 837, 1029
MaeI CTAG 1 cut(s) 449
MaeII ACGT 4 cut(s) 377, 435, 1140, 1152
MaeIII GTNAC 5 cut(s) 43, 295, 361, 490, 745
MboII GAAGA 7 cut(s) 334, 596, 857, 913, 946, 1102, 1105
MflI RGATCY 4 cut(s) 108, 308, 928, 972
MhlI GDGCHC 3 cut(s) 61, 289, 1048
MlsI TGGCCA 1 cut(s) 1229
MluNI TGGCCA 1 cut(s) 1229
Mly113I GGCGCC 1 cut(s) 786
MlyI GAGTC 3 cut(s) 51, 383, 452
MmeI TCCRAC 1 cut(s) 711
Mox20I TGGCCA 1 cut(s) 1229
MscI TGGCCA 1 cut(s) 1229
MseI TTAA 3 cut(s) 645, 933, 1240
MslI CAYNNNNRTG 1 cut(s) 752
Msp20I TGGCCA 1 cut(s) 1229
MspA1I CMGCKG 2 cut(s) 50, 170
MspCI CTTAAG 1 cut(s) 932
MspI CCGG 4 cut(s) 306, 872, 1021, 1066
MspR9I CCNGG 3 cut(s) 282, 986, 1067
MvaI CCWGG 2 cut(s) 282, 986
MvnI CGCG 5 cut(s) 95, 360, 408, 468, 594
MwoI GCNNNNNNNGC 4 cut(s) 56, 357, 419, 538
NarI GGCGCC 1 cut(s) 786
NciI CCSGG 1 cut(s) 1067
NcoI CCATGG 2 cut(s) 235, 609
NlaIII CATG 2 cut(s) 239, 613
NlaIV GGNNCC 5 cut(s) 201, 310, 487, 787, 990
NmeAIII GCCGAG 4 cut(s) 210, 718, 748, 1192
NmuCI GTSAC 4 cut(s) 43, 361, 490, 745
NruI TCGCGA 1 cut(s) 408
OliI CACNNNNGTG 1 cut(s) 752
PcsI WCGNNNNNNNCGW 2 cut(s) 186, 726
PfeI GAWTC 2 cut(s) 15, 1220
PflFI GACNNNGTC 1 cut(s) 392
PflMI CCANNNNNTGG 1 cut(s) 655
PfoI TCCNGGA 1 cut(s) 280
PkrI GCNGC 5 cut(s) 415, 470, 473, 534, 709
Ple19I CGATCG 1 cut(s) 406
PleI GAGTC 3 cut(s) 50, 383, 451
PluTI GGCGCC 1 cut(s) 789
PpsI GAGTC 3 cut(s) 50, 383, 451
Psp124BI GAGCTC 2 cut(s) 61, 289
Psp6I CCWGG 2 cut(s) 280, 984
PspFI CCCAGC 2 cut(s) 75, 537
PspGI CCWGG 2 cut(s) 280, 984
PspN4I GGNNCC 5 cut(s) 201, 310, 487, 787, 990
PspPI GGNCC 3 cut(s) 199, 989, 1063
PstI CTGCAG 1 cut(s) 1185
PstNI CAGNNNCTG 1 cut(s) 1135
PsuI RGATCY 4 cut(s) 108, 308, 928, 972
PsyI GACNNNGTC 1 cut(s) 392
PvuI CGATCG 1 cut(s) 406
RruI TCGCGA 1 cut(s) 408
RsaI GTAC 1 cut(s) 482
RsaNI GTAC 1 cut(s) 481
RseI CAYNNNNRTG 1 cut(s) 752
SacI GAGCTC 2 cut(s) 61, 289
SaqAI TTAA 3 cut(s) 645, 933, 1240
SatI GCNGC 5 cut(s) 414, 469, 472, 533, 708
Sau96I GGNCC 3 cut(s) 199, 989, 1063
SchI GAGTC 3 cut(s) 51, 383, 452
ScrFI CCNGG 3 cut(s) 282, 986, 1067
SduI GDGCHC 3 cut(s) 61, 289, 1048
SfaNI GCATC 5 cut(s) 345, 400, 565, 837, 1029
SfcI CTRYAG 2 cut(s) 701, 1181
SfoI GGCGCC 1 cut(s) 787
SinI GGWCC 2 cut(s) 199, 1063
SmiMI CAYNNNNRTG 1 cut(s) 752
SmlI CTYRAG 2 cut(s) 764, 932
SmoI CTYRAG 2 cut(s) 764, 932
SspDI GGCGCC 1 cut(s) 785
SspMI CTAG 1 cut(s) 449
SstI GAGCTC 2 cut(s) 61, 289
StyD4I CCNGG 3 cut(s) 280, 984, 1065
StyI CCWWGG 2 cut(s) 235, 609
TaaI ACNGT 4 cut(s) 70, 331, 490, 655
TaiI ACGT 4 cut(s) 380, 438, 1143, 1155
TaqI TCGA 6 cut(s) 333, 402, 801, 855, 1030, 1218
TauI GCSGC 5 cut(s) 416, 471, 474, 535, 710
TfiI GAWTC 2 cut(s) 15, 1220
Tru1I TTAA 3 cut(s) 645, 933, 1240
Tru9I TTAA 3 cut(s) 645, 933, 1240
TscAI CASTG 2 cut(s) 73, 389
TseFI GTSAC 4 cut(s) 43, 361, 490, 745
Tsp45I GTSAC 4 cut(s) 43, 361, 490, 745
TspDTI ATGAA 5 cut(s) 511, 597, 628, 914, 1097
TspGWI ACGGA 4 cut(s) 212, 382, 563, 875
TspRI CASTG 2 cut(s) 73, 389
Tth111I GACNNNGTC 1 cut(s) 392
Van91I CCANNNNNTGG 1 cut(s) 655
Vha464I CTTAAG 1 cut(s) 932
VpaK11BI GGWCC 2 cut(s) 199, 1063
XagI CCTNNNNNAGG 1 cut(s) 316
XspI CTAG 1 cut(s) 449
ZraI GACGTC 2 cut(s) 378, 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.