pycom11g26110

NPL4-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0013136
Physical Location & Seq
Reverse (-)
28747258 .. 28750325
3068 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g26110.1

Sequence Viewer

Length: 1248 bp
ATGGCAAAGATGATGCTCCGAGTTCGCAGCAGAGACGGCCTCGAACGAGTGACTGTGGAGAACCCACAGGGCACCACCGTCTCCGAACTCAAATCCCTGATTCAAACCCAGCTCCGAATCCCTTTCCAGAACCAGACCATCTCCACCAACCAGAACCTCCTCTTGGCGAAAACCCAGGAAGAAATCGCCCGATTCACCGACATGGCCAATCCCAATACGCCTCTGACAGCGCTGAATCTTGCACATGGGTCGATTGTGTACTTGGCTTACGACGGCCAGCGCACCGTCGCCGGGCCGGCTTTTCACCCCGCGGGCTCTTTCGGGAGGAAGATGACTATGGATGATTTGATCGCCAATCAGATGAAGGTCACGCGCCAGGAGAACCCTCACTGTGAACTGGTGTCGTTCGATCGGGATTGCGCTAATGCATTCCAGCATTATGTGAGTGAGACGCTGGCTTTTGCGGCGAAGCGGGGAGGGTTTATGTACGGTACGGTGTCGGAGGAGGGGAAGGTGGAGGTGGATTTCATCTACGAGCCTCCGCAGCAGGGGACGGAGGAGAATTTGGTGTTTTACAGAGACCCGGAGGAGGAGAAGGCGGTGGAGGCTATTGCTATGGGGTTGGGTATGAGGCGGGTCGGGTTTATATTCACCCAGTCAGTGAGCCAGGACACAAAGGACTATACTTTGTCGAATAGGGAGGTGCTTCAGGCGGCCGAGTTCCACTCCGAGGGCGGCTTGAAGGAGTGGGTGACCGCCATGGTTAAGTTGGAGGTGAATGAGGATGGTGGTGCTGATGTGCATTTTGAAGCTTTTCAGATGAGTGATATGTGTATTAAATTGTTTAAGGAAGGGTGGTTTGAGACTGAGATTGGAGAGGGTGATGATCCCAAGTTGTCCAAGATGAAGAAGGATGTTGTCCTTGGAGTGAAGGATACTAAGGAGGTGGACAATGATTTTTTCTTGGTGGTAGTAAAGATCTTCGATCACCAGGGGCCACTTTCATCATCCTTTCCTATTGAGAATCGTAACACCCCAGTGACATTGAAGGCTCTGAAAACTCATCTGGATCGTGCTAAGAATCTTCCACTTGTGAAGCGAATTTCGGATTTCCATTTGCTGCTCTTACTGGCCAGGTTTCTAGACGTTGCTAACGATGTTCCTGCTTTGGCAGTGTGTGTCCATACACAATCACCCATACCAGAAGGCTACCAGTTATTGATCGAGACTATGGCCAATACTTCTTAG

Protein Analysis

416

Amino Acids

46.49

Weight (kDa)

5.21

Isoelectric Point (pI)

34.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UN_NPL4 PF11543 3 - 88 4.1e-07 Nuclear pore localisation protein NPL4
NPL4 PF05021 160 - 284 4.1e-11 NPL4 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013136)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47970 AT3G63000
fragaria_vesca FvH4_2g24580
malus_domestica MD03G1274300.v1.1
prunus_persica Prupe.8G251000_v2.0.a1
pyrus_communis pycom03g21870 pycom11g26110
rosa_chinensis RchiOBHm_Chr6g0292401
rosa_laevigata RLG00000012031
rosa_multiflora Rmu_sc0001354.1_g000016 Rmu_sc0012226.1_g000002
rosa_roxburghii Rroxscaffold_7G00175090
rosa_rugosa Rorug06G0226400
rosa_samantha Rh6AG339700 Rh6BG346900 Rh6CG353700 Rh6DG339700
rosa_wichuraiana Rw6G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 71
AccII CGCG 2 cut(s) 311, 373
AclWI GGATC 2 cut(s) 881, 1077
AcoI YGGCCR 5 cut(s) 204, 274, 714, 1131, 1233
AcsI RAATTY 2 cut(s) 562, 1101
AcuI CTGAAG 1 cut(s) 692
AfaI GTAC 3 cut(s) 260, 488, 493
AfeI AGCGCT 1 cut(s) 231
AfiI CCNNNNNNNGG 5 cut(s) 163, 291, 548, 589, 730
AgsI TTSAA 4 cut(s) 104, 742, 809, 1048
AjnI CCWGG 5 cut(s) 174, 375, 666, 990, 1133
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
AleI CACNNNNGTG 1 cut(s) 1037
AluBI AGCT 2 cut(s) 112, 812
AluI AGCT 2 cut(s) 112, 812
Alw26I GTCTC 6 cut(s) 27, 85, 443, 573, 857, 1220
AlwI GGATC 2 cut(s) 881, 1077
Aor51HI AGCGCT 1 cut(s) 231
AoxI GGCC 8 cut(s) 37, 204, 274, 293, 714, 995, 1131, 1233
ApeKI GCWGC 3 cut(s) 27, 544, 1120
ApoI RAATTY 2 cut(s) 562, 1101
Asp700I GAANNNNTTC 1 cut(s) 813
AspLEI GCGC 4 cut(s) 232, 282, 375, 422
AspS9I GGNCC 2 cut(s) 293, 995
AsuC2I CCSGG 2 cut(s) 292, 584
AsuHPI GGTGA 8 cut(s) 187, 296, 643, 763, 787, 893, 980, 1185
BaeGI GKGCMC 1 cut(s) 74
BalI TGGCCA 3 cut(s) 206, 1133, 1235
BanI GGYRCC 1 cut(s) 71
BanII GRGCYC 1 cut(s) 317
BbvI GCAGC 3 cut(s) 39, 556, 1107
BccI CCATC 2 cut(s) 146, 779
BceAI ACGGC 2 cut(s) 52, 289
BcgI CGANNNNNNTGC 4 cut(s) 231, 265, 1145, 1179
BciT130I CCWGG 5 cut(s) 176, 377, 668, 992, 1135
BciVI GTATCC 1 cut(s) 928
BcnI CCSGG 2 cut(s) 292, 584
BcoDI GTCTC 6 cut(s) 27, 85, 443, 573, 857, 1220
BfaI CTAG 1 cut(s) 1142
BfoI RGCGCY 1 cut(s) 233
BfuI GTATCC 1 cut(s) 928
BglI GCCNNNNNGGC 1 cut(s) 296
BglII AGATCT 1 cut(s) 978
BisI GCNGC 6 cut(s) 28, 465, 545, 714, 736, 1121
BlsI GCNGC 6 cut(s) 29, 466, 546, 715, 737, 1122
Bme1390I CCNGG 7 cut(s) 176, 292, 377, 584, 668, 992, 1135
BmgT120I GGNCC 2 cut(s) 293, 995
BmiI GGNNCC 2 cut(s) 73, 996
BmrFI CCNGG 7 cut(s) 176, 292, 377, 584, 668, 992, 1135
BmrI ACTGGG 2 cut(s) 649, 1031
BmsI GCATC 1 cut(s) 3
BmuI ACTGGG 2 cut(s) 649, 1031
BplI GAGNNNNNCTC 4 cut(s) 24, 56, 710, 742
BpuMI CCSGG 2 cut(s) 292, 584
BsaI GGTCTC 1 cut(s) 573
BsaJI CCNNGG 6 cut(s) 174, 309, 729, 759, 922, 991
Bsc4I CCNNNNNNNGG 5 cut(s) 163, 291, 548, 589, 730
Bse118I RCCGGY 1 cut(s) 295
Bse1I ACTGG 5 cut(s) 402, 655, 1037, 1134, 1213
BseBI CCWGG 5 cut(s) 176, 377, 668, 992, 1135
BseDI CCNNGG 6 cut(s) 174, 309, 729, 759, 922, 991
BseGI GGATG 4 cut(s) 346, 790, 919, 1007
BseLI CCNNNNNNNGG 5 cut(s) 163, 291, 548, 589, 730
BseMII CTCAG 1 cut(s) 858
BseNI ACTGG 5 cut(s) 402, 655, 1037, 1134, 1213
BseRI GAGGAG 5 cut(s) 149, 518, 572, 602, 605
BseSI GKGCMC 1 cut(s) 74
BseX3I CGGCCG 1 cut(s) 714
BseXI GCAGC 3 cut(s) 39, 556, 1107
BseYI CCCAGC 1 cut(s) 108
Bsh1236I CGCG 2 cut(s) 311, 373
Bsh1285I CGRYCG 2 cut(s) 412, 717
BshFI GGCC 8 cut(s) 39, 206, 276, 295, 716, 997, 1133, 1235
BshNI GGYRCC 1 cut(s) 71
BsiEI CGRYCG 2 cut(s) 412, 717
BsiSI CCGG 3 cut(s) 291, 296, 584
BslFI GGGAC 1 cut(s) 565
BslI CCNNNNNNNGG 5 cut(s) 163, 291, 548, 589, 730
BsmAI GTCTC 6 cut(s) 27, 85, 443, 573, 857, 1220
BsmBI CGTCTC 3 cut(s) 27, 85, 443
BsmFI GGGAC 1 cut(s) 565
BsmI GAATGC 1 cut(s) 428
BsnI GGCC 8 cut(s) 39, 206, 276, 295, 716, 997, 1133, 1235
Bso31I GGTCTC 1 cut(s) 573
Bsp1286I GDGCHC 2 cut(s) 74, 317
Bsp143I GATC 7 cut(s) 348, 409, 886, 978, 985, 1069, 1221
Bsp19I CCATGG 1 cut(s) 759
BspANI GGCC 8 cut(s) 39, 206, 276, 295, 716, 997, 1133, 1235
BspCNI CTCAG 1 cut(s) 859
BspFNI CGCG 2 cut(s) 311, 373
BspLI GGNNCC 2 cut(s) 73, 996
BspPI GGATC 2 cut(s) 881, 1077
BspT107I GGYRCC 1 cut(s) 71
BspTNI GGTCTC 1 cut(s) 573
BsrFI RCCGGY 1 cut(s) 295
BsrI ACTGG 5 cut(s) 402, 655, 1037, 1134, 1213
BssAI RCCGGY 1 cut(s) 295
BssECI CCNNGG 6 cut(s) 174, 309, 729, 759, 922, 991
BssMI GATC 7 cut(s) 348, 409, 886, 978, 985, 1069, 1221
BssT1I CCWWGG 2 cut(s) 759, 922
Bst2UI CCWGG 5 cut(s) 176, 377, 668, 992, 1135
Bst4CI ACNGT 6 cut(s) 55, 79, 286, 392, 491, 496
BstC8I GCNNGC 4 cut(s) 278, 297, 313, 456
BstDEI CTNAG 4 cut(s) 867, 939, 1077, 1245
BstDSI CCRYGG 2 cut(s) 309, 759
BstEII GGTNACC 1 cut(s) 751
BstF5I GGATG 4 cut(s) 346, 790, 919, 1007
BstFNI CGCG 2 cut(s) 311, 373
BstH2I RGCGCY 1 cut(s) 233
BstHHI GCGC 4 cut(s) 232, 282, 375, 422
BstKTI GATC 7 cut(s) 351, 412, 889, 981, 988, 1072, 1224
BstMAI GTCTC 6 cut(s) 27, 85, 443, 573, 857, 1220
BstMBI GATC 7 cut(s) 348, 409, 886, 978, 985, 1069, 1221
BstMCI CGRYCG 2 cut(s) 412, 717
BstMWI GCNNNNNNNGC 5 cut(s) 36, 296, 464, 544, 605
BstNI CCWGG 5 cut(s) 176, 377, 668, 992, 1135
BstPI GGTNACC 1 cut(s) 751
BstSCI CCNGG 7 cut(s) 174, 290, 375, 582, 666, 990, 1133
BstSLI GKGCMC 1 cut(s) 74
BstUI CGCG 2 cut(s) 311, 373
BstV1I GCAGC 3 cut(s) 39, 556, 1107
BstX2I RGATCY 1 cut(s) 978
BstYI RGATCY 1 cut(s) 978
BstZI CGGCCG 1 cut(s) 714
BsuI GTATCC 1 cut(s) 928
BsuRI GGCC 8 cut(s) 39, 206, 276, 295, 716, 997, 1133, 1235
BtgI CCRYGG 2 cut(s) 309, 759
BtsCI GGATG 4 cut(s) 346, 790, 919, 1007
BtsI GCAGTG 1 cut(s) 1179
BtsIMutI CAGTG 4 cut(s) 388, 666, 1044, 1179
Cac8I GCNNGC 4 cut(s) 278, 297, 313, 456
CfoI GCGC 4 cut(s) 232, 282, 375, 422
Cfr10I RCCGGY 1 cut(s) 295
Cfr13I GGNCC 2 cut(s) 293, 995
Cfr42I CCGCGG 1 cut(s) 312
CseI GACGC 1 cut(s) 460
Csp6I GTAC 3 cut(s) 259, 487, 492
CviAII CATG 3 cut(s) 202, 245, 760
CviQI GTAC 3 cut(s) 259, 487, 492
DdeI CTNAG 4 cut(s) 867, 939, 1077, 1245
DpnI GATC 7 cut(s) 350, 411, 888, 980, 987, 1071, 1223
DpnII GATC 7 cut(s) 348, 409, 886, 978, 985, 1069, 1221
EaeI YGGCCR 5 cut(s) 204, 274, 714, 1131, 1233
EagI CGGCCG 1 cut(s) 714
EclXI CGGCCG 1 cut(s) 714
Eco130I CCWWGG 2 cut(s) 759, 922
Eco24I GRGCYC 1 cut(s) 317
Eco31I GGTCTC 1 cut(s) 573
Eco47III AGCGCT 1 cut(s) 231
Eco52I CGGCCG 1 cut(s) 714
Eco57I CTGAAG 1 cut(s) 692
Eco91I GGTNACC 1 cut(s) 751
EcoO65I GGTNACC 1 cut(s) 751
EcoRII CCWGG 5 cut(s) 174, 375, 666, 990, 1133
EcoT14I CCWWGG 2 cut(s) 759, 922
EcoT22I ATGCAT 1 cut(s) 430
EcoT38I GRGCYC 1 cut(s) 317
ErhI CCWWGG 2 cut(s) 759, 922
Esp3I CGTCTC 3 cut(s) 27, 85, 443
FaeI CATG 3 cut(s) 205, 248, 763
FaqI GGGAC 1 cut(s) 565
FatI CATG 3 cut(s) 201, 244, 759
FauI CCCGC 4 cut(s) 304, 316, 465, 627
Fnu4HI GCNGC 6 cut(s) 28, 465, 545, 714, 736, 1121
FokI GGATG 4 cut(s) 353, 797, 926, 994
FriOI GRGCYC 1 cut(s) 317
Fsp4HI GCNGC 6 cut(s) 28, 465, 545, 714, 736, 1121
FspBI CTAG 1 cut(s) 1142
GlaI GCGC 4 cut(s) 231, 281, 374, 421
GluI GCNGC 6 cut(s) 28, 465, 545, 714, 736, 1121
GsaI CCCAGC 1 cut(s) 112
HaeII RGCGCY 1 cut(s) 233
HaeIII GGCC 8 cut(s) 39, 206, 276, 295, 716, 997, 1133, 1235
HapII CCGG 3 cut(s) 291, 296, 584
HgaI GACGC 1 cut(s) 460
HhaI GCGC 4 cut(s) 232, 282, 375, 422
Hin1II CATG 3 cut(s) 205, 248, 763
Hin6I GCGC 4 cut(s) 230, 280, 373, 420
HinP1I GCGC 4 cut(s) 230, 280, 373, 420
HindIII AAGCTT 1 cut(s) 810
HinfI GANTC 6 cut(s) 100, 117, 192, 235, 1024, 1081
HpaII CCGG 3 cut(s) 291, 296, 584
HphI GGTGA 8 cut(s) 187, 296, 643, 763, 787, 893, 980, 1185
Hpy166II GTNNAC 3 cut(s) 259, 395, 949
Hpy188III TCNNGA 6 cut(s) 127, 322, 413, 1067, 1142, 1225
Hpy8I GTNNAC 3 cut(s) 259, 395, 949
Hpy99I CGWCG 2 cut(s) 275, 290
HpyAV CCTTC 9 cut(s) 358, 505, 589, 736, 845, 904, 925, 1042, 1199
HpyCH4III ACNGT 6 cut(s) 55, 79, 286, 392, 491, 496
HpyCH4IV ACGT 1 cut(s) 1146
HpyCH4V TGCA 3 cut(s) 242, 428, 802
HpyF10VI GCNNNNNNNGC 5 cut(s) 36, 296, 464, 544, 605
HpyF3I CTNAG 4 cut(s) 867, 939, 1077, 1245
HpySE526I ACGT 1 cut(s) 1146
Hsp92II CATG 3 cut(s) 205, 248, 763
HspAI GCGC 4 cut(s) 230, 280, 373, 420
KroI GCCGGC 1 cut(s) 295
KroNI GCCGGC 1 cut(s) 297
KspI CCGCGG 1 cut(s) 312
Kzo9I GATC 7 cut(s) 348, 409, 886, 978, 985, 1069, 1221
LmnI GCTCC 2 cut(s) 21, 117
Lsp1109I GCAGC 3 cut(s) 39, 556, 1107
LweI GCATC 1 cut(s) 3
MaeI CTAG 1 cut(s) 1142
MaeII ACGT 1 cut(s) 1146
MaeIII GTNAC 5 cut(s) 49, 367, 751, 1028, 1039
MalI GATC 7 cut(s) 350, 411, 888, 980, 987, 1071, 1223
MboI GATC 7 cut(s) 348, 409, 886, 978, 985, 1069, 1221
MboII GAAGA 5 cut(s) 191, 340, 919, 973, 1076
MflI RGATCY 1 cut(s) 978
MhlI GDGCHC 2 cut(s) 74, 317
MlsI TGGCCA 3 cut(s) 206, 1133, 1235
MluCI AATT 3 cut(s) 562, 839, 1101
MluNI TGGCCA 3 cut(s) 206, 1133, 1235
MmeI TCCRAC 2 cut(s) 480, 750
Mox20I TGGCCA 3 cut(s) 206, 1133, 1235
Mph1103I ATGCAT 1 cut(s) 430
MroNI GCCGGC 1 cut(s) 295
MroXI GAANNNNTTC 1 cut(s) 813
MscI TGGCCA 3 cut(s) 206, 1133, 1235
MseI TTAA 3 cut(s) 765, 837, 846
MslI CAYNNNNRTG 2 cut(s) 200, 1037
Msp20I TGGCCA 3 cut(s) 206, 1133, 1235
MspA1I CMGCKG 1 cut(s) 311
MspI CCGG 3 cut(s) 291, 296, 584
MspR9I CCNGG 7 cut(s) 176, 292, 377, 584, 668, 992, 1135
Mva1269I GAATGC 1 cut(s) 428
MvaI CCWGG 5 cut(s) 176, 377, 668, 992, 1135
MvnI CGCG 2 cut(s) 311, 373
MwoI GCNNNNNNNGC 5 cut(s) 36, 296, 464, 544, 605
NaeI GCCGGC 1 cut(s) 297
NciI CCSGG 2 cut(s) 292, 584
NcoI CCATGG 1 cut(s) 759
NdeII GATC 7 cut(s) 348, 409, 886, 978, 985, 1069, 1221
NgoMIV GCCGGC 1 cut(s) 295
NlaIII CATG 3 cut(s) 205, 248, 763
NlaIV GGNNCC 2 cut(s) 73, 996
NmeAIII GCCGAG 1 cut(s) 742
NmuCI GTSAC 4 cut(s) 49, 367, 751, 1039
NsiI ATGCAT 1 cut(s) 430
OliI CACNNNNGTG 1 cut(s) 1037
PcsI WCGNNNNNNNCGW 1 cut(s) 1152
PctI GAATGC 1 cut(s) 428
PdiI GCCGGC 1 cut(s) 297
PdmI GAANNNNTTC 1 cut(s) 813
PfeI GAWTC 6 cut(s) 100, 117, 192, 235, 1024, 1081
PkrI GCNGC 6 cut(s) 29, 466, 546, 715, 737, 1122
Ple19I CGATCG 1 cut(s) 412
Psp6I CCWGG 5 cut(s) 174, 375, 666, 990, 1133
PspEI GGTNACC 1 cut(s) 751
PspFI CCCAGC 1 cut(s) 108
PspGI CCWGG 5 cut(s) 174, 375, 666, 990, 1133
PspN4I GGNNCC 2 cut(s) 73, 996
PspPI GGNCC 2 cut(s) 293, 995
PsuI RGATCY 1 cut(s) 978
PvuI CGATCG 1 cut(s) 412
RsaI GTAC 3 cut(s) 260, 488, 493
RsaNI GTAC 3 cut(s) 259, 487, 492
RseI CAYNNNNRTG 2 cut(s) 200, 1037
SacII CCGCGG 1 cut(s) 312
SaqAI TTAA 3 cut(s) 765, 837, 846
SatI GCNGC 6 cut(s) 28, 465, 545, 714, 736, 1121
Sau3AI GATC 7 cut(s) 348, 409, 886, 978, 985, 1069, 1221
Sau96I GGNCC 2 cut(s) 293, 995
ScrFI CCNGG 7 cut(s) 176, 292, 377, 584, 668, 992, 1135
SduI GDGCHC 2 cut(s) 74, 317
SfaNI GCATC 1 cut(s) 3
Sfr303I CCGCGG 1 cut(s) 312
SgrBI CCGCGG 1 cut(s) 312
SmiMI CAYNNNNRTG 2 cut(s) 200, 1037
Sse9I AATT 3 cut(s) 562, 839, 1101
SspMI CTAG 1 cut(s) 1142
StyD4I CCNGG 7 cut(s) 174, 290, 375, 582, 666, 990, 1133
StyI CCWWGG 2 cut(s) 759, 922
TaaI ACNGT 6 cut(s) 55, 79, 286, 392, 491, 496
TaiI ACGT 1 cut(s) 1149
TaqI TCGA 6 cut(s) 42, 251, 408, 692, 984, 1224
TasI AATT 3 cut(s) 562, 839, 1101
TatI WGTACW 1 cut(s) 258
TauI GCSGC 3 cut(s) 467, 716, 738
TfiI GAWTC 6 cut(s) 100, 117, 192, 235, 1024, 1081
Tru1I TTAA 3 cut(s) 765, 837, 846
Tru9I TTAA 3 cut(s) 765, 837, 846
TscAI CASTG 4 cut(s) 395, 666, 1044, 1179
TseFI GTSAC 4 cut(s) 49, 367, 751, 1039
TseI GCWGC 3 cut(s) 27, 544, 1120
Tsp45I GTSAC 4 cut(s) 49, 367, 751, 1039
TspDTI ATGAA 4 cut(s) 377, 517, 920, 993
TspGWI ACGGA 1 cut(s) 569
TspRI CASTG 4 cut(s) 395, 666, 1044, 1179
XapI RAATTY 2 cut(s) 562, 1101
XbaI TCTAGA 1 cut(s) 1141
XcmI CCANNNNNNNNNTGG 1 cut(s) 766
XmnI GAANNNNTTC 1 cut(s) 813
XspI CTAG 1 cut(s) 1142
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.