AT3G63000

npl4-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013136
Physical Location & Seq
Forward (+)
23283592 .. 23285540
1949 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G63000.1

Sequence Viewer

Length: 1242 bp
ATGACGATGCTCAGAGTCCGAAGCAGAGATGGATTGGAGAGAGTGAGTGTAGATGGACCTCATATCACAGTCTCCCAACTCAAAACCCTAATCCAAGATCAGCTTCAGATCCCTATCCACAACCAAACCTTATCCACCAATAGAAACCTTCTCCTCGCTAAGAGCCCTTCTGATTTCCTAGCGTTCACCGACATGGCCGATCCTAACCTCCGAATCTCGTCTCTCAATCTAGCTCACGGCTCTATGGTCTACCTTGCTTACGAAGGTGAGCGTACGATTCGTGGTGGTCCTGCTGTAACGCCTGCTGGTTCCTTCGGGAGGAAGATGACCGTCGAAGATCTAATCGCTCGACAGATGCGCGTCGGTCGCCAGGAGAAGGCTCACTGCGATTCCGTCTCGTTCGATCGCGATTGCGCCAATGCGTTTCAGCATTTCGTCAACGAGTCGCTTGCCTTCGCTGTCAAACGCGGTGGATTCATGTACGGGAACGTCTCTGAGGATGGTCAGGTTGAGGTGAATTTCATCTACGAGCCACCGCAGCAAGGGATGGAGGATAATCTGATTCTGATGAGAGACTCTGAGGAAGAGAAACGTGTTGATGCTATTGCTTTAGGGTTAGGGATGAGGAGAGTTGGGTTTATATTCAACCAGACTGTGACTCAGGACAAGAAGGAGTACACTTTGTCTAACGTTGAGGTGTTGCTTGCGGCTCAGCTTCACGCTGAGAGCGAATTGAAGGAGTGGGTCACTGCTGTTGTGAAGCTTGAGATCAATGAGGATGGTGGTGCTGATGTTCACTTTGAGCCGTTTCAGATGAGTGATATGTGCGTTAGGTTGTTTAAGGAAGGATGGTTCGAGACTGAGATTGGACCTGAGGATGATCCCAAGCTCTCCAAGTTGAAGAAAGAGGTTGTTGTTGGAGTCAAGGATGTTAAAGAAGTCGATAATGATTTCTTCCTTGTTCTCGTCAAGATCTTGGATCATCAGGGACCGTTGTCGTGTACCTTCCCGATAGAGAACAGGAACACTCAAACAACAATGCGGGCTTTGAAAACGCATATGGAGCGAGCAAGGAGCCTCCCATTCGTGAAGAGGATCTCTGATTTTCATCTGCTTCTCTTTGTGGCTCAGTTTCTAGACGTATCATCCGACGTCCCTGCGTTGGCTGAATGCGTCCGTCTACAGTCTCATGTCCCTGAGGGATATGAGTTGCTCATCGACTCCATGGCCAATACTTCTTAA

Protein Analysis

413

Amino Acids

46.5

Weight (kDa)

5.27

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UN_NPL4 PF11543 3 - 86 1.9e-07 Nuclear pore localisation protein NPL4
NPL4 PF05021 159 - 290 7.4e-13 NPL4 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013136)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47970 AT3G63000
fragaria_vesca FvH4_2g24580
malus_domestica MD03G1274300.v1.1
prunus_persica Prupe.8G251000_v2.0.a1
pyrus_communis pycom03g21870 pycom11g26110
rosa_chinensis RchiOBHm_Chr6g0292401
rosa_laevigata RLG00000012031
rosa_multiflora Rmu_sc0001354.1_g000016 Rmu_sc0012226.1_g000002
rosa_roxburghii Rroxscaffold_7G00175090
rosa_rugosa Rorug06G0226400
rosa_samantha Rh6AG339700 Rh6BG346900 Rh6CG353700 Rh6DG339700
rosa_wichuraiana Rw6G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1155
AccI GTMKAC 2 cut(s) 249, 1180
AccII CGCG 3 cut(s) 360, 408, 468
AciI CCGC 4 cut(s) 468, 536, 707, 1042
AclI AACGTT 1 cut(s) 690
AclWI GGATC 5 cut(s) 103, 194, 875, 987, 1103
AcoI YGGCCR 2 cut(s) 195, 1227
AcsI RAATTY 1 cut(s) 517
AcuI CTGAAG 1 cut(s) 89
AcyI GRCGYC 1 cut(s) 1152
AfaI GTAC 4 cut(s) 274, 482, 677, 1003
AfiI CCNNNNNNNGG 4 cut(s) 318, 376, 542, 1162
AflIII ACRYGT 1 cut(s) 592
AgsI TTSAA 4 cut(s) 646, 736, 901, 1051
AjnI CCWGG 1 cut(s) 369
AjuI GAANNNNNNNTTGG 2 cut(s) 87, 119
AloI GAACNNNNNNTCC 2 cut(s) 836, 868
AluBI AGCT 5 cut(s) 103, 233, 715, 763, 889
AluI AGCT 5 cut(s) 103, 233, 715, 763, 889
Alw26I GTCTC 7 cut(s) 76, 225, 400, 496, 567, 851, 1191
AlwI GGATC 5 cut(s) 103, 194, 875, 987, 1103
AoxI GGCC 2 cut(s) 195, 1227
ApeKI GCWGC 1 cut(s) 538
ApoI RAATTY 1 cut(s) 517
AspLEI GCGC 2 cut(s) 360, 416
AspS9I GGNCC 4 cut(s) 56, 287, 869, 989
AsuHPI GGTGA 3 cut(s) 178, 278, 526
AvaII GGWCC 4 cut(s) 56, 287, 869, 989
AxyI CCTNAGG 2 cut(s) 873, 1197
BalI TGGCCA 1 cut(s) 1229
BanII GRGCYC 1 cut(s) 167
BbvI GCAGC 1 cut(s) 550
BccI CCATC 6 cut(s) 23, 47, 494, 541, 773, 843
BceAI ACGGC 2 cut(s) 253, 790
BcgI CGANNNNNNTGC 4 cut(s) 431, 465, 1139, 1173
BciT130I CCWGG 1 cut(s) 371
BcoDI GTCTC 7 cut(s) 76, 225, 400, 496, 567, 851, 1191
BfaI CTAG 3 cut(s) 179, 230, 1136
BfmI CTRYAG 1 cut(s) 1181
BglII AGATCT 2 cut(s) 337, 972
BisI GCNGC 2 cut(s) 539, 708
BlpI GCTNAGC 1 cut(s) 711
BlsI GCNGC 2 cut(s) 540, 709
Bme1390I CCNGG 1 cut(s) 371
Bme18I GGWCC 4 cut(s) 56, 287, 869, 989
BmgT120I GGNCC 4 cut(s) 56, 287, 869, 989
BmiI GGNNCC 3 cut(s) 310, 990, 1076
BmrFI CCNGG 1 cut(s) 371
BmsI GCATC 2 cut(s) 345, 589
BoxI GACNNNNGTC 1 cut(s) 994
Bpu1102I GCTNAGC 1 cut(s) 711
BpuEI CTTGAG 1 cut(s) 785
BsaBI GATNNNNATC 2 cut(s) 113, 1107
BsaHI GRCGYC 1 cut(s) 1152
BsaJI CCNNGG 1 cut(s) 1224
Bsc4I CCNNNNNNNGG 4 cut(s) 318, 376, 542, 1162
Bse21I CCTNAGG 2 cut(s) 873, 1197
Bse8I GATNNNNATC 2 cut(s) 113, 1107
BseBI CCWGG 1 cut(s) 371
BseDI CCNNGG 1 cut(s) 1224
BseGI GGATG 8 cut(s) 505, 552, 627, 784, 854, 883, 934, 1145
BseJI GATNNNNATC 2 cut(s) 113, 1107
BseLI CCNNNNNNNGG 4 cut(s) 318, 376, 542, 1162
BseRI GAGGAG 2 cut(s) 143, 640
BseXI GCAGC 1 cut(s) 550
Bsh1236I CGCG 3 cut(s) 360, 408, 468
Bsh1285I CGRYCG 2 cut(s) 367, 406
BshFI GGCC 2 cut(s) 197, 1229
BsiEI CGRYCG 2 cut(s) 367, 406
BsiWI CGTACG 1 cut(s) 272
BslFI GGGAC 3 cut(s) 1002, 1139, 1178
BslI CCNNNNNNNGG 4 cut(s) 318, 376, 542, 1162
BsmAI GTCTC 7 cut(s) 76, 225, 400, 496, 567, 851, 1191
BsmBI CGTCTC 3 cut(s) 225, 400, 496
BsmFI GGGAC 3 cut(s) 1002, 1139, 1178
BsmI GAATGC 1 cut(s) 1175
BsnI GGCC 2 cut(s) 197, 1229
Bsp1286I GDGCHC 1 cut(s) 167
Bsp1720I GCTNAGC 1 cut(s) 711
Bsp19I CCATGG 1 cut(s) 1224
Bsp68I TCGCGA 1 cut(s) 408
BspACI CCGC 4 cut(s) 468, 536, 707, 1042
BspANI GGCC 2 cut(s) 197, 1229
BspFNI CGCG 3 cut(s) 360, 408, 468
BspLI GGNNCC 3 cut(s) 310, 990, 1076
BspPI GGATC 5 cut(s) 103, 194, 875, 987, 1103
BssECI CCNNGG 1 cut(s) 1224
BssNI GRCGYC 1 cut(s) 1152
BssT1I CCWWGG 1 cut(s) 1224
Bst2UI CCWGG 1 cut(s) 371
Bst4CI ACNGT 5 cut(s) 70, 331, 655, 993, 1185
Bst6I CTCTTC 2 cut(s) 579, 1085
BstACI GRCGYC 1 cut(s) 1152
BstC8I GCNNGC 5 cut(s) 303, 450, 705, 1044, 1068
BstDSI CCRYGG 1 cut(s) 1224
BstENI CCTNNNNNAGG 1 cut(s) 316
BstF5I GGATG 8 cut(s) 505, 552, 627, 784, 854, 883, 934, 1145
BstFNI CGCG 3 cut(s) 360, 408, 468
BstHHI GCGC 2 cut(s) 360, 416
BstMAI GTCTC 7 cut(s) 76, 225, 400, 496, 567, 851, 1191
BstMCI CGRYCG 2 cut(s) 367, 406
BstMWI GCNNNNNNNGC 3 cut(s) 366, 538, 1063
BstNI CCWGG 1 cut(s) 371
BstPAI GACNNNNGTC 1 cut(s) 994
BstSCI CCNGG 1 cut(s) 369
BstSFI CTRYAG 1 cut(s) 1181
BstUI CGCG 3 cut(s) 360, 408, 468
BstV1I GCAGC 1 cut(s) 550
BstX2I RGATCY 4 cut(s) 108, 337, 972, 1095
BstYI RGATCY 4 cut(s) 108, 337, 972, 1095
Bsu36I CCTNAGG 2 cut(s) 873, 1197
BsuRI GGCC 2 cut(s) 197, 1229
BtgI CCRYGG 1 cut(s) 1224
BtsCI GGATG 8 cut(s) 505, 552, 627, 784, 854, 883, 934, 1145
BtsI GCAGTG 2 cut(s) 382, 747
BtsIMutI CAGTG 2 cut(s) 382, 747
BtuMI TCGCGA 1 cut(s) 408
Cac8I GCNNGC 5 cut(s) 303, 450, 705, 1044, 1068
CfoI GCGC 2 cut(s) 360, 416
Cfr13I GGNCC 4 cut(s) 56, 287, 869, 989
CseI GACGC 2 cut(s) 349, 1162
Csp6I GTAC 4 cut(s) 273, 481, 676, 1002
CspCI CAANNNNNGTGG 2 cut(s) 451, 486
CviAII CATG 4 cut(s) 193, 478, 1190, 1225
CviQI GTAC 4 cut(s) 273, 481, 676, 1002
EaeI YGGCCR 2 cut(s) 195, 1227
Eam1104I CTCTTC 2 cut(s) 579, 1085
EarI CTCTTC 2 cut(s) 579, 1085
Eco130I CCWWGG 1 cut(s) 1224
Eco24I GRGCYC 1 cut(s) 167
Eco47I GGWCC 4 cut(s) 56, 287, 869, 989
Eco57I CTGAAG 1 cut(s) 89
Eco81I CCTNAGG 2 cut(s) 873, 1197
EcoNI CCTNNNNNAGG 1 cut(s) 316
EcoRII CCWGG 1 cut(s) 369
EcoT14I CCWWGG 1 cut(s) 1224
EcoT38I GRGCYC 1 cut(s) 167
ErhI CCWWGG 1 cut(s) 1224
Esp3I CGTCTC 3 cut(s) 225, 400, 496
FaeI CATG 4 cut(s) 196, 481, 1193, 1228
FalI AAGNNNNNCTT 2 cut(s) 87, 119
FaqI GGGAC 3 cut(s) 1002, 1139, 1178
FatI CATG 4 cut(s) 192, 477, 1189, 1224
FauI CCCGC 1 cut(s) 1035
FauNDI CATATG 1 cut(s) 1059
FblI GTMKAC 2 cut(s) 249, 1180
Fnu4HI GCNGC 2 cut(s) 539, 708
FokI GGATG 8 cut(s) 512, 559, 634, 791, 861, 890, 941, 1132
FriOI GRGCYC 1 cut(s) 167
Fsp4HI GCNGC 2 cut(s) 539, 708
FspBI CTAG 3 cut(s) 179, 230, 1136
GlaI GCGC 2 cut(s) 359, 415
GluI GCNGC 2 cut(s) 539, 708
HaeIII GGCC 2 cut(s) 197, 1229
HgaI GACGC 2 cut(s) 349, 1162
HhaI GCGC 2 cut(s) 360, 416
Hin1I GRCGYC 1 cut(s) 1152
Hin1II CATG 4 cut(s) 196, 481, 1193, 1228
Hin6I GCGC 2 cut(s) 358, 414
HinP1I GCGC 2 cut(s) 358, 414
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HindIII AAGCTT 1 cut(s) 761
HphI GGTGA 3 cut(s) 178, 278, 526
Hpy166II GTNNAC 7 cut(s) 186, 250, 439, 678, 796, 1002, 1181
Hpy188III TCNNGA 8 cut(s) 316, 407, 662, 856, 970, 1009, 1087, 1136
Hpy8I GTNNAC 7 cut(s) 186, 250, 439, 678, 796, 1002, 1181
Hpy99I CGWCG 3 cut(s) 335, 365, 1154
HpyCH4III ACNGT 5 cut(s) 70, 331, 655, 993, 1185
HpyCH4IV ACGT 5 cut(s) 489, 592, 690, 1140, 1152
HpyF10VI GCNNNNNNNGC 3 cut(s) 366, 538, 1063
HpySE526I ACGT 5 cut(s) 489, 592, 690, 1140, 1152
Hsp92I GRCGYC 1 cut(s) 1152
Hsp92II CATG 4 cut(s) 196, 481, 1193, 1228
HspAI GCGC 2 cut(s) 358, 414
LmnI GCTCC 2 cut(s) 1063, 1074
Lsp1109I GCAGC 1 cut(s) 550
LweI GCATC 2 cut(s) 345, 589
MaeI CTAG 3 cut(s) 179, 230, 1136
MaeII ACGT 5 cut(s) 489, 592, 690, 1140, 1152
MaeIII GTNAC 3 cut(s) 295, 655, 745
MboII GAAGA 6 cut(s) 334, 347, 596, 913, 946, 1102
MflI RGATCY 4 cut(s) 108, 337, 972, 1095
MhlI GDGCHC 1 cut(s) 167
MlsI TGGCCA 1 cut(s) 1229
MluCI AATT 2 cut(s) 517, 731
MluNI TGGCCA 1 cut(s) 1229
MlyI GAGTC 6 cut(s) 24, 452, 569, 652, 930, 1214
MmeI TCCRAC 2 cut(s) 898, 1173
Mox20I TGGCCA 1 cut(s) 1229
MscI TGGCCA 1 cut(s) 1229
MseI TTAA 3 cut(s) 840, 933, 1240
MslI CAYNNNNRTG 1 cut(s) 191
Msp20I TGGCCA 1 cut(s) 1229
MspR9I CCNGG 1 cut(s) 371
Mva1269I GAATGC 1 cut(s) 1175
MvaI CCWGG 1 cut(s) 371
MvnI CGCG 3 cut(s) 360, 408, 468
MwoI GCNNNNNNNGC 3 cut(s) 366, 538, 1063
NcoI CCATGG 1 cut(s) 1224
NdeI CATATG 1 cut(s) 1059
NlaIII CATG 4 cut(s) 196, 481, 1193, 1228
NlaIV GGNNCC 3 cut(s) 310, 990, 1076
NmuCI GTSAC 2 cut(s) 655, 745
NruI TCGCGA 1 cut(s) 408
PcsI WCGNNNNNNNCGW 2 cut(s) 726, 1146
PctI GAATGC 1 cut(s) 1175
PfeI GAWTC 5 cut(s) 213, 277, 389, 474, 562
Pfl23II CGTACG 1 cut(s) 272
PkrI GCNGC 2 cut(s) 540, 709
Ple19I CGATCG 1 cut(s) 406
PleI GAGTC 6 cut(s) 23, 451, 569, 652, 929, 1214
PpsI GAGTC 6 cut(s) 23, 451, 569, 652, 929, 1214
PshAI GACNNNNGTC 1 cut(s) 994
Psp1406I AACGTT 1 cut(s) 690
Psp6I CCWGG 1 cut(s) 369
PspGI CCWGG 1 cut(s) 369
PspLI CGTACG 1 cut(s) 272
PspN4I GGNNCC 3 cut(s) 310, 990, 1076
PspPI GGNCC 4 cut(s) 56, 287, 869, 989
PsuI RGATCY 4 cut(s) 108, 337, 972, 1095
PvuI CGATCG 1 cut(s) 406
RruI TCGCGA 1 cut(s) 408
RsaI GTAC 4 cut(s) 274, 482, 677, 1003
RsaNI GTAC 4 cut(s) 273, 481, 676, 1002
RseI CAYNNNNRTG 1 cut(s) 191
SaqAI TTAA 3 cut(s) 840, 933, 1240
SatI GCNGC 2 cut(s) 539, 708
Sau96I GGNCC 4 cut(s) 56, 287, 869, 989
SchI GAGTC 6 cut(s) 24, 452, 569, 652, 930, 1214
ScrFI CCNGG 1 cut(s) 371
SduI GDGCHC 1 cut(s) 167
SfaNI GCATC 2 cut(s) 345, 589
SfcI CTRYAG 1 cut(s) 1181
SinI GGWCC 4 cut(s) 56, 287, 869, 989
SmiMI CAYNNNNRTG 1 cut(s) 191
SmlI CTYRAG 1 cut(s) 764
SmoI CTYRAG 1 cut(s) 764
Sse9I AATT 2 cut(s) 517, 731
SsiI CCGC 4 cut(s) 468, 536, 707, 1042
SspMI CTAG 3 cut(s) 179, 230, 1136
StyD4I CCNGG 1 cut(s) 369
StyI CCWWGG 1 cut(s) 1224
TaaI ACNGT 5 cut(s) 70, 331, 655, 993, 1185
TaiI ACGT 5 cut(s) 492, 595, 693, 1143, 1155
TaqI TCGA 6 cut(s) 333, 349, 402, 855, 942, 1218
TaqII GACCGA 1 cut(s) 353
TasI AATT 2 cut(s) 517, 731
TatI WGTACW 1 cut(s) 675
TauI GCSGC 1 cut(s) 710
TfiI GAWTC 5 cut(s) 213, 277, 389, 474, 562
Tru1I TTAA 3 cut(s) 840, 933, 1240
Tru9I TTAA 3 cut(s) 840, 933, 1240
TscAI CASTG 2 cut(s) 389, 754
TseFI GTSAC 2 cut(s) 655, 745
TseI GCWGC 1 cut(s) 538
Tsp45I GTSAC 2 cut(s) 655, 745
TspDTI ATGAA 3 cut(s) 466, 511, 1097
TspGWI ACGGA 2 cut(s) 382, 1166
TspRI CASTG 2 cut(s) 389, 754
VpaK11BI GGWCC 4 cut(s) 56, 287, 869, 989
XagI CCTNNNNNAGG 1 cut(s) 316
XapI RAATTY 1 cut(s) 517
XbaI TCTAGA 1 cut(s) 1135
XmiI GTMKAC 2 cut(s) 249, 1180
XspI CTAG 3 cut(s) 179, 230, 1136
ZraI GACGTC 1 cut(s) 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.