AT3G01060

Domain of unknown function (DUF4336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0015474
Physical Location & Seq
Reverse (-)
16631 .. 18909
2279 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G01060.2

Sequence Viewer

Length: 1368 bp
ATGGCGGCAGCGGCCATGGCCGTTCATCTCCCGAAACATTCTTCGTTTCTTCCGAAACCCAAACTTCCATTGGATAAAAAAACCAACTTTCTCGGTGGGTCATTTAATTTAGGTCGACCCATGTCCGTAAACCGGGAATTTAACAGTCCGGTGATGGTCTCCGCTTCTTCGACGAGCAAGACCGTCCTTACCGATGACGTAGACGGCGATCAAAGTAAACGCTTTTATATTAACTTCACTGGCTTTCCTTTTCCTCTTGGTCCTTTCCTTAACCGGCGCACCATCAGAACCGAGGCGGTTAAAGGTTGCATATGGATGTTTGAACAAGAACAAGCTTTAGGGTTTAGCAGCGTGTCCACTAATATACGAATGACTGTCATCAAACTAAAATCTGGAGGTTTATGGGTTCATGCTCCTATTGCTCCCACCAAAGAGTGTGTCCAGCTTATTAAGGAGTTGGGAGCTCCGGTTGAGTACATTGTCCTGCCAACGTTTGCTTATGAGCACAAGATTTTTGTCGGTCCTTTTTCTAGAAAGTTCCCCAAGGCTCAAGTATGGGTTGCACCAAGACAATGGAGCTGGCCATTGAACTTACCACTTGAGTTTTTCGGTATCTTTCGTGCTAAAATCATCAAAGACAGGGACTTATCTACGCCATGGGCTGAGGAGATCGAACAGAAAGTCCTAAGCTCCCCAGAAGTTGGAATTGGACCATATGTCGAAGTAGCGTTCTACCATAAGCGGTCAAGAACCCTTTTAGTCACTGATGCTGTGATCTTCGTCCCGCGGAAACCTCCCTCGAGTATCAGCAACGAATCCTTGTTGGCCTCGGCTAAGAATGGACTAGCTGTGAAGATACTTAGCAAAGGAAAAGACATTCCTAATGATCCTGTGGTTGATAATCCTAACACCCGCCAAAAAGGATGGGAAAGAATGGTTCTGCAAATCCTATTTCTTGGTCCCTCGAATCTCTTGGAACCAAACGCAAGCTTCGCACGAATGTCACAGAAGCTGATCGTTTCTCCCATTGTAAAGACTTTGGTCTTTAGCAAAGTCCCTGAGAAGGTGAGGGATTGGATCGATGAGATAGCCCGTGACTGGAGATTTAAAAGGATTATCCCAGCTCATTTCGAAGCTCCAATAAACGCAGGAAGATCAGATTTTCTAGCTGCGTTTGGGTTCCTAGAGGATCTTCTAGGGGAAAGATATGTAACTCGTCCTTCGCTCTCTCTTCTCTTCACTTCACTAATGGGAAAAGCTGCGAGCTATTTTCCTCCAGACGATATGAGAACTCTCTCTTCACTTGATCAGTTCTTAGTCTCTGTTGGGGCTGTGAAAAAGACAGTATCCGGTAGAAAACGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4336

Protein Analysis

455

Amino Acids

51.02

Weight (kDa)

9.89

Isoelectric Point (pI)

42.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4336 PF14234 101 - 316 1.7e-77 Domain of unknown function (DUF4336)
DUF4336 PF14234 321 - 386 3.2e-19 Domain of unknown function (DUF4336)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01060 AT3G01060
fragaria_vesca FvH4_6g47510
malus_domestica MD17G1056600.v1.1
prunus_persica Prupe.3G258900_v2.0.a1
pyrus_communis pycom17g05560
rosa_chinensis RchiOBHm_Chr2g0166541
rosa_laevigata RLG00000021628
rosa_multiflora Rmu_sc0010842.1_g000007
rosa_roxburghii Rroxscaffold_2G00084760
rosa_rugosa Rorug02G0524100
rosa_samantha Rh2AG590000 Rh2BG602100 Rh2CG572800 Rh2DG612700
rosa_wichuraiana Rw2G049180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 701
AccI GTMKAC 2 cut(s) 115, 201
AccII CGCG 1 cut(s) 787
AciI CCGC 8 cut(s) 5, 11, 162, 296, 742, 785, 787, 913
AclI AACGTT 1 cut(s) 491
AclWI GGATC 3 cut(s) 881, 1085, 1197
AcoI YGGCCR 3 cut(s) 12, 18, 581
AcsI RAATTY 1 cut(s) 137
AfaI GTAC 1 cut(s) 476
AfiI CCNNNNNNNGG 4 cut(s) 132, 701, 1063, 1098
AgsI TTSAA 2 cut(s) 323, 589
AhdI GACNNNNNGTC 1 cut(s) 716
AjuI GAANNNNNNNTTGG 2 cut(s) 690, 722
AloI GAACNNNNNNTCC 2 cut(s) 666, 698
Alw21I GWGCWC 2 cut(s) 466, 507
Alw26I GTCTC 2 cut(s) 163, 1324
AlwI GGATC 3 cut(s) 881, 1085, 1197
AlwNI CAGNNNCTG 1 cut(s) 1012
Ama87I CYCGRG 1 cut(s) 799
AoxI GGCC 4 cut(s) 12, 18, 581, 825
ApeKI GCWGC 4 cut(s) 8, 348, 1169, 1259
ApoI RAATTY 1 cut(s) 137
ArsI GACNNNNNNTTYG 4 cut(s) 423, 455, 666, 698
AspLEI GCGC 1 cut(s) 279
AspS9I GGNCC 4 cut(s) 260, 521, 710, 959
AsuC2I CCSGG 1 cut(s) 134
AsuHPI GGTGA 2 cut(s) 163, 1078
AsuII TTCGAA 1 cut(s) 1131
AvaI CYCGRG 1 cut(s) 799
AvaII GGWCC 4 cut(s) 260, 521, 710, 959
BalI TGGCCA 1 cut(s) 583
BanII GRGCYC 1 cut(s) 466
Bbv12I GWGCWC 2 cut(s) 466, 507
BbvCI CCTCAGC 1 cut(s) 663
BbvI GCAGC 4 cut(s) 20, 360, 1156, 1246
BccI CCATC 3 cut(s) 148, 290, 918
BceAI ACGGC 2 cut(s) 5, 220
BciVI GTATCC 1 cut(s) 1357
BclI TGATCA 1 cut(s) 1306
BcnI CCSGG 1 cut(s) 134
BcoDI GTCTC 2 cut(s) 163, 1324
BfaI CTAG 5 cut(s) 531, 845, 1166, 1184, 1196
BfuI GTATCC 1 cut(s) 1357
BisI GCNGC 6 cut(s) 6, 9, 12, 349, 1170, 1260
BlsI GCNGC 6 cut(s) 7, 10, 13, 350, 1171, 1261
Bme1390I CCNGG 1 cut(s) 134
Bme18I GGWCC 4 cut(s) 260, 521, 710, 959
BmeRI GACNNNNNGTC 1 cut(s) 716
BmeT110I CYCGRG 1 cut(s) 799
BmgT120I GGNCC 4 cut(s) 260, 521, 710, 959
BmiI GGNNCC 3 cut(s) 961, 978, 1181
BmrFI CCNGG 1 cut(s) 134
BmsI GCATC 1 cut(s) 757
BoxI GACNNNNGTC 2 cut(s) 121, 1040
BpmI CTGGAG 3 cut(s) 414, 1120, 1260
Bpu10I CCTNAGC 2 cut(s) 663, 686
Bpu14I TTCGAA 1 cut(s) 1131
BpuEI CTTGAG 2 cut(s) 534, 620
BpuMI CCSGG 1 cut(s) 134
Bsa29I ATCGAT 1 cut(s) 1080
BsaI GGTCTC 1 cut(s) 163
BsaJI CCNNGG 6 cut(s) 15, 291, 543, 656, 785, 828
BsaWI WCCGGW 3 cut(s) 148, 466, 1349
BsaXI ACNNNNNCTCC 2 cut(s) 453, 483
Bsc4I CCNNNNNNNGG 4 cut(s) 132, 701, 1063, 1098
Bse118I RCCGGY 1 cut(s) 273
Bse1I ACTGG 2 cut(s) 244, 1103
BseCI ATCGAT 1 cut(s) 1080
BseDI CCNNGG 6 cut(s) 15, 291, 543, 656, 785, 828
BseGI GGATG 2 cut(s) 321, 929
BseLI CCNNNNNNNGG 4 cut(s) 132, 701, 1063, 1098
BseMII CTCAG 2 cut(s) 654, 1050
BseNI ACTGG 2 cut(s) 244, 1103
BseRI GAGGAG 1 cut(s) 680
BseXI GCAGC 4 cut(s) 20, 360, 1156, 1246
BseYI CCCAGC 1 cut(s) 1120
Bsh1236I CGCG 1 cut(s) 787
BshFI GGCC 4 cut(s) 14, 20, 583, 827
BshVI ATCGAT 1 cut(s) 1080
BsiHKAI GWGCWC 2 cut(s) 466, 507
BsiHKCI CYCGRG 1 cut(s) 799
BsiSI CCGG 5 cut(s) 133, 149, 274, 467, 1350
BslFI GGGAC 4 cut(s) 656, 767, 945, 1040
BslI CCNNNNNNNGG 4 cut(s) 132, 701, 1063, 1098
BsmAI GTCTC 2 cut(s) 163, 1324
BsmFI GGGAC 4 cut(s) 656, 767, 945, 1040
BsnI GGCC 4 cut(s) 14, 20, 583, 827
Bso31I GGTCTC 1 cut(s) 163
BsoBI CYCGRG 1 cut(s) 799
Bsp119I TTCGAA 1 cut(s) 1131
Bsp1286I GDGCHC 2 cut(s) 466, 507
Bsp143I GATC 9 cut(s) 208, 669, 774, 886, 1014, 1077, 1154, 1189, 1306
Bsp19I CCATGG 2 cut(s) 15, 656
BspACI CCGC 8 cut(s) 5, 11, 162, 296, 742, 785, 787, 913
BspANI GGCC 4 cut(s) 14, 20, 583, 827
BspCNI CTCAG 2 cut(s) 655, 1051
BspDI ATCGAT 1 cut(s) 1080
BspFNI CGCG 1 cut(s) 787
BspLI GGNNCC 3 cut(s) 961, 978, 1181
BspPI GGATC 3 cut(s) 881, 1085, 1197
BspT104I TTCGAA 1 cut(s) 1131
BspTNI GGTCTC 1 cut(s) 163
BsrFI RCCGGY 1 cut(s) 273
BsrI ACTGG 2 cut(s) 244, 1103
BssAI RCCGGY 1 cut(s) 273
BssECI CCNNGG 6 cut(s) 15, 291, 543, 656, 785, 828
BssMI GATC 9 cut(s) 208, 669, 774, 886, 1014, 1077, 1154, 1189, 1306
BssT1I CCWWGG 3 cut(s) 15, 543, 656
Bst4CI ACNGT 4 cut(s) 146, 184, 376, 1345
Bst6I CTCTTC 3 cut(s) 1236, 1241, 1303
BstBI TTCGAA 1 cut(s) 1131
BstC8I GCNNGC 3 cut(s) 581, 988, 1264
BstDEI CTNAG 6 cut(s) 663, 686, 834, 860, 1059, 1315
BstDSI CCRYGG 3 cut(s) 15, 656, 785
BstF5I GGATG 2 cut(s) 321, 929
BstFNI CGCG 1 cut(s) 787
BstHHI GCGC 1 cut(s) 279
BstKTI GATC 9 cut(s) 211, 672, 777, 889, 1017, 1080, 1157, 1192, 1309
BstMAI GTCTC 2 cut(s) 163, 1324
BstMBI GATC 9 cut(s) 208, 669, 774, 886, 1014, 1077, 1154, 1189, 1306
BstMWI GCNNNNNNNGC 4 cut(s) 11, 17, 419, 992
BstPAI GACNNNNGTC 2 cut(s) 121, 1040
BstSCI CCNGG 1 cut(s) 132
BstUI CGCG 1 cut(s) 787
BstV1I GCAGC 4 cut(s) 20, 360, 1156, 1246
BstX2I RGATCY 1 cut(s) 1189
BstXI CCANNNNNNTGG 1 cut(s) 573
BstYI RGATCY 1 cut(s) 1189
Bsu15I ATCGAT 1 cut(s) 1080
BsuI GTATCC 1 cut(s) 1357
BsuRI GGCC 4 cut(s) 14, 20, 583, 827
BsuTUI ATCGAT 1 cut(s) 1080
BtgI CCRYGG 3 cut(s) 15, 656, 785
BtsCI GGATG 2 cut(s) 321, 929
BtsIMutI CAGTG 2 cut(s) 237, 762
Cac8I GCNNGC 3 cut(s) 581, 988, 1264
CaiI CAGNNNCTG 1 cut(s) 1012
CfoI GCGC 1 cut(s) 279
Cfr10I RCCGGY 1 cut(s) 273
Cfr13I GGNCC 4 cut(s) 260, 521, 710, 959
Cfr42I CCGCGG 1 cut(s) 788
ClaI ATCGAT 1 cut(s) 1080
Csp6I GTAC 1 cut(s) 475
CviAII CATG 4 cut(s) 16, 121, 410, 657
CviQI GTAC 1 cut(s) 475
DdeI CTNAG 6 cut(s) 663, 686, 834, 860, 1059, 1315
DpnI GATC 9 cut(s) 210, 671, 776, 888, 1016, 1079, 1156, 1191, 1308
DpnII GATC 9 cut(s) 208, 669, 774, 886, 1014, 1077, 1154, 1189, 1306
DraI TTTAAA 1 cut(s) 1108
DriI GACNNNNNGTC 1 cut(s) 716
EaeI YGGCCR 3 cut(s) 12, 18, 581
Eam1104I CTCTTC 3 cut(s) 1236, 1241, 1303
Eam1105I GACNNNNNGTC 1 cut(s) 716
EarI CTCTTC 3 cut(s) 1236, 1241, 1303
Ecl136II GAGCTC 1 cut(s) 464
Eco130I CCWWGG 3 cut(s) 15, 543, 656
Eco24I GRGCYC 1 cut(s) 466
Eco31I GGTCTC 1 cut(s) 163
Eco47I GGWCC 4 cut(s) 260, 521, 710, 959
Eco53kI GAGCTC 1 cut(s) 464
Eco88I CYCGRG 1 cut(s) 799
EcoICRI GAGCTC 1 cut(s) 464
EcoT14I CCWWGG 3 cut(s) 15, 543, 656
EcoT38I GRGCYC 1 cut(s) 466
ErhI CCWWGG 3 cut(s) 15, 543, 656
FaeI CATG 4 cut(s) 19, 124, 413, 660
FaqI GGGAC 4 cut(s) 656, 767, 945, 1040
FatI CATG 4 cut(s) 15, 120, 409, 656
FauI CCCGC 2 cut(s) 792, 920
FauNDI CATATG 2 cut(s) 311, 715
FbaI TGATCA 1 cut(s) 1306
FblI GTMKAC 2 cut(s) 115, 201
Fnu4HI GCNGC 6 cut(s) 6, 9, 12, 349, 1170, 1260
FokI GGATG 2 cut(s) 328, 936
FriOI GRGCYC 1 cut(s) 466
Fsp4HI GCNGC 6 cut(s) 6, 9, 12, 349, 1170, 1260
FspBI CTAG 5 cut(s) 531, 845, 1166, 1184, 1196
GlaI GCGC 1 cut(s) 278
GluI GCNGC 6 cut(s) 6, 9, 12, 349, 1170, 1260
GsaI CCCAGC 1 cut(s) 1124
GsuI CTGGAG 3 cut(s) 414, 1120, 1260
HaeIII GGCC 4 cut(s) 14, 20, 583, 827
HapII CCGG 5 cut(s) 133, 149, 274, 467, 1350
HhaI GCGC 1 cut(s) 279
Hin1II CATG 4 cut(s) 19, 124, 413, 660
Hin6I GCGC 1 cut(s) 277
HinP1I GCGC 1 cut(s) 277
HincII GTYRAC 1 cut(s) 116
HindII GTYRAC 1 cut(s) 116
HindIII AAGCTT 2 cut(s) 333, 988
HinfI GANTC 2 cut(s) 815, 967
HpaII CCGG 5 cut(s) 133, 149, 274, 467, 1350
HphI GGTGA 2 cut(s) 163, 1078
Hpy166II GTNNAC 5 cut(s) 116, 130, 202, 218, 357
Hpy188I TCNGA 3 cut(s) 54, 287, 1159
Hpy188III TCNNGA 5 cut(s) 31, 393, 531, 747, 1277
Hpy8I GTNNAC 5 cut(s) 116, 130, 202, 218, 357
Hpy99I CGWCG 1 cut(s) 175
HpyAV CCTTC 2 cut(s) 1057, 1230
HpyCH4III ACNGT 4 cut(s) 146, 184, 376, 1345
HpyCH4IV ACGT 2 cut(s) 198, 491
HpyCH4V TGCA 3 cut(s) 309, 563, 943
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 17, 419, 992
HpyF3I CTNAG 6 cut(s) 663, 686, 834, 860, 1059, 1315
HpySE526I ACGT 2 cut(s) 198, 491
Hsp92II CATG 4 cut(s) 19, 124, 413, 660
HspAI GCGC 1 cut(s) 277
Ksp22I TGATCA 1 cut(s) 1306
KspI CCGCGG 1 cut(s) 788
Kzo9I GATC 9 cut(s) 208, 669, 774, 886, 1014, 1077, 1154, 1189, 1306
LmnI GCTCC 7 cut(s) 418, 427, 461, 469, 576, 695, 1141
Lsp1109I GCAGC 4 cut(s) 20, 360, 1156, 1246
LweI GCATC 1 cut(s) 757
MaeI CTAG 5 cut(s) 531, 845, 1166, 1184, 1196
MaeII ACGT 2 cut(s) 198, 491
MaeIII GTNAC 4 cut(s) 760, 1002, 1094, 1210
MalI GATC 9 cut(s) 210, 671, 776, 888, 1016, 1079, 1156, 1191, 1308
MboI GATC 9 cut(s) 208, 669, 774, 886, 1014, 1077, 1154, 1189, 1306
MflI RGATCY 1 cut(s) 1189
MhlI GDGCHC 2 cut(s) 466, 507
MlsI TGGCCA 1 cut(s) 583
MluCI AATT 3 cut(s) 106, 137, 705
MluNI TGGCCA 1 cut(s) 583
MmeI TCCRAC 1 cut(s) 682
Mox20I TGGCCA 1 cut(s) 583
MscI TGGCCA 1 cut(s) 583
MseI TTAA 7 cut(s) 105, 141, 231, 270, 300, 450, 1107
MslI CAYNNNNRTG 1 cut(s) 314
Msp20I TGGCCA 1 cut(s) 583
MspA1I CMGCKG 2 cut(s) 11, 787
MspI CCGG 5 cut(s) 133, 149, 274, 467, 1350
MspR9I CCNGG 1 cut(s) 134
MvnI CGCG 1 cut(s) 787
MwoI GCNNNNNNNGC 4 cut(s) 11, 17, 419, 992
NciI CCSGG 1 cut(s) 134
NcoI CCATGG 2 cut(s) 15, 656
NdeI CATATG 2 cut(s) 311, 715
NdeII GATC 9 cut(s) 208, 669, 774, 886, 1014, 1077, 1154, 1189, 1306
NlaIII CATG 4 cut(s) 19, 124, 413, 660
NlaIV GGNNCC 3 cut(s) 961, 978, 1181
NmeAIII GCCGAG 1 cut(s) 809
NmuCI GTSAC 3 cut(s) 760, 1002, 1094
NspV TTCGAA 1 cut(s) 1131
PaeR7I CTCGAG 1 cut(s) 799
PcsI WCGNNNNNNNCGW 2 cut(s) 50, 204
PfeI GAWTC 2 cut(s) 815, 967
PflMI CCANNNNNTGG 1 cut(s) 701
PkrI GCNGC 6 cut(s) 7, 10, 13, 350, 1171, 1261
PshAI GACNNNNGTC 2 cut(s) 121, 1040
Psp124BI GAGCTC 1 cut(s) 466
Psp1406I AACGTT 1 cut(s) 491
PspFI CCCAGC 1 cut(s) 1120
PspN4I GGNNCC 3 cut(s) 961, 978, 1181
PspPI GGNCC 4 cut(s) 260, 521, 710, 959
PspXI VCTCGAGB 1 cut(s) 799
PstNI CAGNNNCTG 1 cut(s) 1012
PsuI RGATCY 1 cut(s) 1189
RsaI GTAC 1 cut(s) 476
RsaNI GTAC 1 cut(s) 475
RseI CAYNNNNRTG 1 cut(s) 314
SacI GAGCTC 1 cut(s) 466
SacII CCGCGG 1 cut(s) 788
SalI GTCGAC 1 cut(s) 114
SaqAI TTAA 7 cut(s) 105, 141, 231, 270, 300, 450, 1107
SatI GCNGC 6 cut(s) 6, 9, 12, 349, 1170, 1260
Sau3AI GATC 9 cut(s) 208, 669, 774, 886, 1014, 1077, 1154, 1189, 1306
Sau96I GGNCC 4 cut(s) 260, 521, 710, 959
ScrFI CCNGG 1 cut(s) 134
SduI GDGCHC 2 cut(s) 466, 507
SfaNI GCATC 1 cut(s) 757
Sfr274I CTCGAG 1 cut(s) 799
Sfr303I CCGCGG 1 cut(s) 788
SfuI TTCGAA 1 cut(s) 1131
SgrBI CCGCGG 1 cut(s) 788
SinI GGWCC 4 cut(s) 260, 521, 710, 959
SlaI CTCGAG 1 cut(s) 799
SmiMI CAYNNNNRTG 1 cut(s) 314
SmlI CTYRAG 3 cut(s) 549, 599, 799
SmoI CTYRAG 3 cut(s) 549, 599, 799
Sse9I AATT 3 cut(s) 106, 137, 705
SsiI CCGC 8 cut(s) 5, 11, 162, 296, 742, 785, 787, 913
SspMI CTAG 5 cut(s) 531, 845, 1166, 1184, 1196
SstI GAGCTC 1 cut(s) 466
StyD4I CCNGG 1 cut(s) 132
StyI CCWWGG 3 cut(s) 15, 543, 656
TaaI ACNGT 4 cut(s) 146, 184, 376, 1345
TaiI ACGT 2 cut(s) 201, 494
TaqI TCGA 8 cut(s) 115, 170, 672, 720, 800, 965, 1080, 1131
TaqII GACCGA 1 cut(s) 509
TasI AATT 3 cut(s) 106, 137, 705
TatI WGTACW 1 cut(s) 474
TauI GCSGC 2 cut(s) 8, 14
TfiI GAWTC 2 cut(s) 815, 967
Tru1I TTAA 7 cut(s) 105, 141, 231, 270, 300, 450, 1107
Tru9I TTAA 7 cut(s) 105, 141, 231, 270, 300, 450, 1107
TscAI CASTG 2 cut(s) 244, 769
TseFI GTSAC 3 cut(s) 760, 1002, 1094
TseI GCWGC 4 cut(s) 8, 348, 1169, 1259
Tsp45I GTSAC 3 cut(s) 760, 1002, 1094
TspDTI ATGAA 2 cut(s) 14, 398
TspGWI ACGGA 1 cut(s) 115
TspRI CASTG 2 cut(s) 244, 769
Van91I CCANNNNNTGG 1 cut(s) 701
VpaK11BI GGWCC 4 cut(s) 260, 521, 710, 959
XapI RAATTY 1 cut(s) 137
XbaI TCTAGA 1 cut(s) 530
XcmI CCANNNNNNNNNTGG 1 cut(s) 67
XhoI CTCGAG 1 cut(s) 799
XmiI GTMKAC 2 cut(s) 115, 201
XspI CTAG 5 cut(s) 531, 845, 1166, 1184, 1196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.