pycom17g05560

Domain of unknown function (DUF4336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0015474
Physical Location & Seq
Forward (+)
3960596 .. 3963314
2719 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g05560.6

Sequence Viewer

Length: 1647 bp
ATGAAATTCATAATCTTGCTCAAGAAATATACCATCGATGAAATTGGTTCGAAACTTCTCGATGCTTCCTCCTTTTCTCCTCAAGAAGTTATTTATCTAGCCTGTCTATCCCTACCATCCACAATAATTCTAAAAATTCTTATAAAATCCATGCCAGACAATCGGACTGGATCCTCCACGTGTCAAAAGCACAACCTGGTATTGCAACCTATGACTATGCATTATGTTATGCACCACTCCTCTCCTAGTGGTGCCGAAGGCAAAGTGCTAACAAAAAGTTTCAGAAAAACAATGGCAGCCATTGCCGTTTCTTCTCCAAAATCCTCCATTTTTCAGAACCCAATCTCTCGCAGAGAGCCAAGTTCCAGTCTTTTTGGCGGGTCCTTGAAGGGTTTTTGTTTTCAGTTGAGACCCGGAAACAAGAGCAGAAATGGCATCAATTTGGTGGTTGCTGCCACAAACTCCACCACCATCAGTGGCAGCAGAGGCCGCGGTGGCGACCGGTTTTATATCAACTTCACCGGGTTTCCTTTTCCACTGGGTCCTTTTCTCAATAGAAGCACTACTAGGACTGAGGCTGTGAAAGGCTGCATATGGTTATTTGAGCAAGAGCAAGCATTAGGCTTCAGCAGTGTGTCAACCAACATTAGAATGACAGTCATCAAACTAAAATCCGGAGGATTGTGGGTCCATGCGCCCATCGCTCCAACCAAAGAGTGTATTCAGCTTCTGAAAGAGTTGGGGGCTCCCGTAGAATACATAGTTCTTCCTACATTTGCTTATGAGCACAAAATTTTTGTTGGTCCGTTCTCTAGAGAGTTCCCTCGAGCTCAGGTATGGGTGGCACCAAGGCAATGGAGTTGGCCTTTGAATCTCCCGCTGGAGTTTTTTGGGATCTTTCGTGCTAAAACCTTAAAAGACGAGGATTTTTCCACTCCATGGACGGATGAGATTGAGCAGAAAGTTCTCAGCTCACCAGAAGTTGGTATCGGGCCATATGTGGAGGTAGCTTTCTATCATAAGCGTTCCAGAACTCTACTGGTAACAGATGCTGTAATTTTTGTGCCAAGACAGCCACCTGATTGTATCAGCAAAGAATCCTTGTTGGCATCTGCAAAGAATGGCTTGGCGGTAAAACTTCTTAGCAAAGGAAAAAAAGTATCTCAAGAACCAGTAGTTGACAACAAGATGAACCGTCAAAAAGGTTGGGAACGAATGGTTCTTCAAATTTTGTTTCTTGGTCCATCAAATCTGTTGGAACCGAATGCTAGCTTTGCTCAGATGTCACAGAAGCTGATTGTTTCACCCATTGTAAAGACTCTTGTCTTCAACAAAGTTCCTGAAAAGGTCAGGGATTGGGTTGATGGAATCGCGCGGGACTGGAGGTTCAAGAGAATAATCCCTGCTCATTTTGCAGCACCAATCAACGCAGGCAGGTCTGATTTGTTAGCTGCATTTGCCTTTCTGGATGACCTTCTGGATGAGCCTTATGTCACTCGCCCATCACTCTCTCTTCTCTTCACATCACTATTGGGAAAGGCTGCGAGCTATTTCCCTCCCGATGACATGAAGACTTTATCATCCCTCGATCAGTTTTTGGTCTCGGTTGGAGTTGTGAAAAAGACCGTCTCAGGGCGCAAACGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4336

Protein Analysis

549

Amino Acids

61.02

Weight (kDa)

9.8

Isoelectric Point (pI)

48.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01060 AT3G01060
fragaria_vesca FvH4_6g47510
malus_domestica MD17G1056600.v1.1
prunus_persica Prupe.3G258900_v2.0.a1
pyrus_communis pycom17g05560
rosa_chinensis RchiOBHm_Chr2g0166541
rosa_laevigata RLG00000021628
rosa_multiflora Rmu_sc0010842.1_g000007
rosa_roxburghii Rroxscaffold_2G00084760
rosa_rugosa Rorug02G0524100
rosa_samantha Rh2AG590000 Rh2BG602100 Rh2CG572800 Rh2DG612700
rosa_wichuraiana Rw2G049180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 143
Acc36I ACCTGC 1 cut(s) 1425
AccB1I GGYRCC 2 cut(s) 251, 844
AccB7I CCANNNNNTGG 2 cut(s) 939, 983
AccII CGCG 3 cut(s) 492, 1373, 1375
AccIII TCCGGA 1 cut(s) 674
AciI CCGC 6 cut(s) 378, 490, 492, 878, 1130, 1375
AclWI GGATC 3 cut(s) 165, 178, 902
AcsI RAATTY 4 cut(s) 5, 135, 792, 1227
AcuI CTGAAG 1 cut(s) 610
AcvI CACGTG 1 cut(s) 180
AfiI CCNNNNNNNGG 3 cut(s) 939, 983, 1632
AflIII ACRYGT 1 cut(s) 179
AgeI ACCGGT 1 cut(s) 501
AgsI TTSAA 5 cut(s) 388, 871, 1226, 1330, 1390
AjnI CCWGG 1 cut(s) 195
AloI GAACNNNNNNTCC 2 cut(s) 1370, 1402
AluBI AGCT 8 cut(s) 727, 830, 972, 1010, 1272, 1294, 1451, 1548
AluI AGCT 8 cut(s) 727, 830, 972, 1010, 1272, 1294, 1451, 1548
Alw21I GWGCWC 2 cut(s) 789, 832
Alw26I GTCTC 3 cut(s) 403, 1606, 1633
AlwI GGATC 3 cut(s) 165, 178, 902
AlwNI CAGNNNCTG 3 cut(s) 730, 1052, 1294
Ama87I CYCGRG 1 cut(s) 825
Aor13HI TCCGGA 1 cut(s) 674
AoxI GGCC 3 cut(s) 487, 863, 992
ApeKI GCWGC 7 cut(s) 296, 452, 480, 588, 1415, 1451, 1541
ApoI RAATTY 4 cut(s) 5, 135, 792, 1227
AsiGI ACCGGT 1 cut(s) 501
AspLEI GCGC 3 cut(s) 697, 1375, 1638
AspS9I GGNCC 6 cut(s) 381, 542, 688, 803, 992, 1241
AsuC2I CCSGG 2 cut(s) 414, 523
AsuHPI GGTGA 3 cut(s) 511, 966, 1296
AsuII TTCGAA 1 cut(s) 50
AsuNHI GCTAGC 1 cut(s) 1268
AvaI CYCGRG 1 cut(s) 825
AvaII GGWCC 5 cut(s) 381, 542, 688, 803, 1241
BamHI GGATCC 1 cut(s) 170
BanI GGYRCC 2 cut(s) 251, 844
BanII GRGCYC 2 cut(s) 748, 832
BbrPI CACGTG 1 cut(s) 180
BbsI GAAGAC 2 cut(s) 1318, 1577
Bbv12I GWGCWC 2 cut(s) 789, 832
BbvI GCAGC 7 cut(s) 308, 439, 492, 575, 1427, 1438, 1528
BccI CCATC 7 cut(s) 41, 124, 479, 707, 1252, 1358, 1510
BceAI ACGGC 1 cut(s) 290
BciT130I CCWGG 1 cut(s) 197
BcnI CCSGG 2 cut(s) 414, 523
BcoDI GTCTC 3 cut(s) 403, 1606, 1633
BfaI CTAG 5 cut(s) 98, 246, 567, 813, 1269
BfuAI ACCTGC 1 cut(s) 1425
BglI GCCNNNNNGGC 1 cut(s) 495
BisI GCNGC 8 cut(s) 297, 453, 481, 490, 589, 1416, 1452, 1542
BlsI GCNGC 8 cut(s) 298, 454, 482, 491, 590, 1417, 1453, 1543
Bme1390I CCNGG 3 cut(s) 197, 414, 523
Bme18I GGWCC 5 cut(s) 381, 542, 688, 803, 1241
BmeT110I CYCGRG 1 cut(s) 825
BmgT120I GGNCC 6 cut(s) 381, 542, 688, 803, 992, 1241
BmiI GGNNCC 8 cut(s) 172, 253, 382, 543, 689, 747, 846, 1260
BmrFI CCNGG 3 cut(s) 197, 414, 523
BmrI ACTGGG 1 cut(s) 548
BmsI GCATC 4 cut(s) 52, 444, 1039, 1118
BmtI GCTAGC 1 cut(s) 1272
BmuI ACTGGG 1 cut(s) 548
BoxI GACNNNNGTC 1 cut(s) 1322
BpiI GAAGAC 2 cut(s) 1318, 1577
BpmI CTGGAG 2 cut(s) 902, 1402
Bpu10I CCTNAGC 1 cut(s) 831
Bpu14I TTCGAA 1 cut(s) 50
BpuEI CTTGAG 3 cut(s) 5, 66, 1149
BpuMI CCSGG 2 cut(s) 414, 523
Bsa29I ATCGAT 1 cut(s) 36
BsaAI YACGTR 1 cut(s) 180
BsaI GGTCTC 2 cut(s) 403, 1606
BsaJI CCNNGG 3 cut(s) 490, 848, 938
BsaWI WCCGGW 2 cut(s) 501, 674
Bsc4I CCNNNNNNNGG 3 cut(s) 939, 983, 1632
Bse118I RCCGGY 1 cut(s) 501
Bse1I ACTGG 6 cut(s) 172, 366, 543, 1044, 1172, 1385
Bse3DI GCAATG 2 cut(s) 300, 860
BseAI TCCGGA 1 cut(s) 674
BseBI CCWGG 1 cut(s) 197
BseCI ATCGAT 1 cut(s) 36
BseDI CCNNGG 3 cut(s) 490, 848, 938
BseGI GGATG 5 cut(s) 116, 952, 1474, 1486, 1580
BseLI CCNNNNNNNGG 3 cut(s) 939, 983, 1632
BseMI GCAATG 2 cut(s) 300, 860
BseMII CTCAG 5 cut(s) 564, 845, 982, 1292, 1644
BseNI ACTGG 6 cut(s) 172, 366, 543, 1044, 1172, 1385
BseRI GAGGAG 2 cut(s) 69, 229
BseXI GCAGC 7 cut(s) 308, 439, 492, 575, 1427, 1438, 1528
Bsh1236I CGCG 3 cut(s) 492, 1373, 1375
Bsh1285I CGRYCG 1 cut(s) 502
BshFI GGCC 3 cut(s) 489, 865, 994
BshNI GGYRCC 2 cut(s) 251, 844
BshTI ACCGGT 1 cut(s) 501
BshVI ATCGAT 1 cut(s) 36
BsiEI CGRYCG 1 cut(s) 502
BsiHKAI GWGCWC 2 cut(s) 789, 832
BsiHKCI CYCGRG 1 cut(s) 825
BsiSI CCGG 4 cut(s) 414, 502, 522, 675
BslFI GGGAC 1 cut(s) 1391
BslI CCNNNNNNNGG 3 cut(s) 939, 983, 1632
BsmAI GTCTC 3 cut(s) 403, 1606, 1633
BsmBI CGTCTC 1 cut(s) 1633
BsmFI GGGAC 1 cut(s) 1391
BsmI GAATGC 1 cut(s) 1270
BsnI GGCC 3 cut(s) 489, 865, 994
Bso31I GGTCTC 2 cut(s) 403, 1606
BsoBI CYCGRG 1 cut(s) 825
Bsp119I TTCGAA 1 cut(s) 50
Bsp1286I GDGCHC 3 cut(s) 748, 789, 832
Bsp13I TCCGGA 1 cut(s) 674
Bsp143I GATC 3 cut(s) 170, 894, 1588
Bsp19I CCATGG 1 cut(s) 938
BspACI CCGC 6 cut(s) 378, 490, 492, 878, 1130, 1375
BspANI GGCC 3 cut(s) 489, 865, 994
BspCNI CTCAG 5 cut(s) 565, 844, 981, 1291, 1643
BspDI ATCGAT 1 cut(s) 36
BspEI TCCGGA 1 cut(s) 674
BspFNI CGCG 3 cut(s) 492, 1373, 1375
BspLI GGNNCC 8 cut(s) 172, 253, 382, 543, 689, 747, 846, 1260
BspMI ACCTGC 1 cut(s) 1425
BspOI GCTAGC 1 cut(s) 1272
BspPI GGATC 3 cut(s) 165, 178, 902
BspT104I TTCGAA 1 cut(s) 50
BspT107I GGYRCC 2 cut(s) 251, 844
BspTNI GGTCTC 2 cut(s) 403, 1606
BsrDI GCAATG 2 cut(s) 300, 860
BsrFI RCCGGY 1 cut(s) 501
BsrI ACTGG 6 cut(s) 172, 366, 543, 1044, 1172, 1385
BssAI RCCGGY 1 cut(s) 501
BssECI CCNNGG 3 cut(s) 490, 848, 938
BssMI GATC 3 cut(s) 170, 894, 1588
BssT1I CCWWGG 2 cut(s) 848, 938
Bst2UI CCWGG 1 cut(s) 197
Bst4CI ACNGT 3 cut(s) 658, 1196, 1627
Bst6I CTCTTC 2 cut(s) 1518, 1523
BstAPI GCANNNNNTGC 1 cut(s) 302
BstBAI YACGTR 1 cut(s) 180
BstBI TTCGAA 1 cut(s) 50
BstC8I GCNNGC 4 cut(s) 615, 1270, 1432, 1546
BstDEI CTNAG 6 cut(s) 573, 831, 968, 1142, 1278, 1630
BstDSI CCRYGG 2 cut(s) 490, 938
BstF5I GGATG 5 cut(s) 116, 952, 1474, 1486, 1580
BstFNI CGCG 3 cut(s) 492, 1373, 1375
BstHHI GCGC 3 cut(s) 697, 1375, 1638
BstKTI GATC 3 cut(s) 173, 897, 1591
BstMAI GTCTC 3 cut(s) 403, 1606, 1633
BstMBI GATC 3 cut(s) 170, 894, 1588
BstMCI CGRYCG 1 cut(s) 502
BstNI CCWGG 1 cut(s) 197
BstPAI GACNNNNGTC 1 cut(s) 1322
BstSCI CCNGG 3 cut(s) 195, 412, 521
BstUI CGCG 3 cut(s) 492, 1373, 1375
BstV1I GCAGC 7 cut(s) 308, 439, 492, 575, 1427, 1438, 1528
BstV2I GAAGAC 2 cut(s) 1318, 1577
BstX2I RGATCY 2 cut(s) 170, 894
BstXI CCANNNNNNTGG 1 cut(s) 855
BstYI RGATCY 2 cut(s) 170, 894
Bsu15I ATCGAT 1 cut(s) 36
BsuRI GGCC 3 cut(s) 489, 865, 994
BsuTUI ATCGAT 1 cut(s) 36
BtgI CCRYGG 2 cut(s) 490, 938
BtgZI GCGATG 1 cut(s) 685
BtsCI GGATG 5 cut(s) 116, 952, 1474, 1486, 1580
BtsI GCAGTG 1 cut(s) 637
BtsIMutI CAGTG 3 cut(s) 481, 536, 637
BveI ACCTGC 1 cut(s) 1425
Cac8I GCNNGC 4 cut(s) 615, 1270, 1432, 1546
CaiI CAGNNNCTG 3 cut(s) 730, 1052, 1294
CfoI GCGC 3 cut(s) 697, 1375, 1638
Cfr10I RCCGGY 1 cut(s) 501
Cfr13I GGNCC 6 cut(s) 381, 542, 688, 803, 992, 1241
Cfr42I CCGCGG 1 cut(s) 493
ClaI ATCGAT 1 cut(s) 36
CsiI ACCWGGT 1 cut(s) 195
CspAI ACCGGT 1 cut(s) 501
CviAII CATG 4 cut(s) 151, 692, 939, 1567
DdeI CTNAG 6 cut(s) 573, 831, 968, 1142, 1278, 1630
DpnI GATC 3 cut(s) 172, 896, 1590
DpnII GATC 3 cut(s) 170, 894, 1588
Eam1104I CTCTTC 2 cut(s) 1518, 1523
EarI CTCTTC 2 cut(s) 1518, 1523
Ecl136II GAGCTC 1 cut(s) 830
Eco130I CCWWGG 2 cut(s) 848, 938
Eco24I GRGCYC 2 cut(s) 748, 832
Eco31I GGTCTC 2 cut(s) 403, 1606
Eco47I GGWCC 5 cut(s) 381, 542, 688, 803, 1241
Eco53kI GAGCTC 1 cut(s) 830
Eco57I CTGAAG 1 cut(s) 610
Eco72I CACGTG 1 cut(s) 180
Eco88I CYCGRG 1 cut(s) 825
EcoICRI GAGCTC 1 cut(s) 830
EcoO109I RGGNCCY 2 cut(s) 381, 542
EcoRII CCWGG 1 cut(s) 195
EcoT14I CCWWGG 2 cut(s) 848, 938
EcoT22I ATGCAT 1 cut(s) 222
EcoT38I GRGCYC 2 cut(s) 748, 832
ErhI CCWWGG 2 cut(s) 848, 938
Esp3I CGTCTC 1 cut(s) 1633
FaeI CATG 4 cut(s) 154, 695, 942, 1570
FalI AAGNNNNNCTT 2 cut(s) 1109, 1141
FaqI GGGAC 1 cut(s) 1391
FatI CATG 4 cut(s) 150, 691, 938, 1566
FauI CCCGC 3 cut(s) 371, 885, 1368
FauNDI CATATG 2 cut(s) 593, 997
Fnu4HI GCNGC 8 cut(s) 297, 453, 481, 490, 589, 1416, 1452, 1542
FokI GGATG 5 cut(s) 103, 959, 1481, 1493, 1567
FriOI GRGCYC 2 cut(s) 748, 832
Fsp4HI GCNGC 8 cut(s) 297, 453, 481, 490, 589, 1416, 1452, 1542
FspBI CTAG 5 cut(s) 98, 246, 567, 813, 1269
GlaI GCGC 3 cut(s) 696, 1374, 1637
GluI GCNGC 8 cut(s) 297, 453, 481, 490, 589, 1416, 1452, 1542
GsuI CTGGAG 2 cut(s) 902, 1402
HaeIII GGCC 3 cut(s) 489, 865, 994
HapII CCGG 4 cut(s) 414, 502, 522, 675
HhaI GCGC 3 cut(s) 697, 1375, 1638
Hin1II CATG 4 cut(s) 154, 695, 942, 1570
Hin6I GCGC 3 cut(s) 695, 1373, 1636
HinP1I GCGC 3 cut(s) 695, 1373, 1636
HincII GTYRAC 2 cut(s) 639, 1180
HindII GTYRAC 2 cut(s) 639, 1180
HinfI GANTC 4 cut(s) 871, 1097, 1318, 1368
HpaII CCGG 4 cut(s) 414, 502, 522, 675
HphI GGTGA 3 cut(s) 511, 966, 1296
Hpy166II GTNNAC 2 cut(s) 639, 1180
Hpy188I TCNGA 6 cut(s) 165, 284, 336, 732, 1281, 1441
Hpy8I GTNNAC 2 cut(s) 639, 1180
HpyAV CCTTC 3 cut(s) 251, 382, 1484
HpyCH4III ACNGT 3 cut(s) 658, 1196, 1627
HpyCH4IV ACGT 1 cut(s) 179
HpyCH4V TGCA 7 cut(s) 205, 220, 232, 591, 1115, 1415, 1454
HpyF3I CTNAG 6 cut(s) 573, 831, 968, 1142, 1278, 1630
HpySE526I ACGT 1 cut(s) 179
Hsp92II CATG 4 cut(s) 154, 695, 942, 1570
HspAI GCGC 3 cut(s) 695, 1373, 1636
Kpn2I TCCGGA 1 cut(s) 674
KspI CCGCGG 1 cut(s) 493
Kzo9I GATC 3 cut(s) 170, 894, 1588
LmnI GCTCC 2 cut(s) 709, 751
Lsp1109I GCAGC 7 cut(s) 308, 439, 492, 575, 1427, 1438, 1528
LweI GCATC 4 cut(s) 52, 444, 1039, 1118
MabI ACCWGGT 1 cut(s) 195
MaeI CTAG 5 cut(s) 98, 246, 567, 813, 1269
MaeII ACGT 1 cut(s) 179
MaeIII GTNAC 3 cut(s) 1042, 1284, 1492
MalI GATC 3 cut(s) 172, 896, 1590
MboI GATC 3 cut(s) 170, 894, 1588
MboII GAAGA 7 cut(s) 303, 758, 1214, 1318, 1505, 1510, 1582
MflI RGATCY 2 cut(s) 170, 894
MhlI GDGCHC 3 cut(s) 748, 789, 832
MluCI AATT 8 cut(s) 5, 42, 126, 135, 439, 792, 1056, 1227
MlyI GAGTC 1 cut(s) 1312
MmeI TCCRAC 3 cut(s) 731, 1236, 1588
Mph1103I ATGCAT 1 cut(s) 222
MroI TCCGGA 1 cut(s) 674
MseI TTAA 1 cut(s) 914
MslI CAYNNNNRTG 1 cut(s) 650
MspA1I CMGCKG 2 cut(s) 492, 880
MspI CCGG 4 cut(s) 414, 502, 522, 675
MspR9I CCNGG 3 cut(s) 197, 414, 523
Mva1269I GAATGC 1 cut(s) 1270
MvaI CCWGG 1 cut(s) 197
MvnI CGCG 3 cut(s) 492, 1373, 1375
NciI CCSGG 2 cut(s) 414, 523
NcoI CCATGG 1 cut(s) 938
NdeI CATATG 2 cut(s) 593, 997
NdeII GATC 3 cut(s) 170, 894, 1588
NheI GCTAGC 1 cut(s) 1268
NlaIII CATG 4 cut(s) 154, 695, 942, 1570
NlaIV GGNNCC 8 cut(s) 172, 253, 382, 543, 689, 747, 846, 1260
NmuCI GTSAC 2 cut(s) 1284, 1492
NsiI ATGCAT 1 cut(s) 222
NspV TTCGAA 1 cut(s) 50
PaeR7I CTCGAG 1 cut(s) 825
PctI GAATGC 1 cut(s) 1270
PfeI GAWTC 3 cut(s) 871, 1097, 1368
PflMI CCANNNNNTGG 2 cut(s) 939, 983
PinAI ACCGGT 1 cut(s) 501
PkrI GCNGC 8 cut(s) 298, 454, 482, 491, 590, 1417, 1453, 1543
PleI GAGTC 1 cut(s) 1312
PmaCI CACGTG 1 cut(s) 180
PmlI CACGTG 1 cut(s) 180
PpsI GAGTC 1 cut(s) 1312
Ppu21I YACGTR 1 cut(s) 180
PpuMI RGGWCCY 2 cut(s) 381, 542
PshAI GACNNNNGTC 1 cut(s) 1322
PsiI TTATAA 1 cut(s) 143
Psp124BI GAGCTC 1 cut(s) 832
Psp5II RGGWCCY 2 cut(s) 381, 542
Psp6I CCWGG 1 cut(s) 195
PspCI CACGTG 1 cut(s) 180
PspGI CCWGG 1 cut(s) 195
PspN4I GGNNCC 8 cut(s) 172, 253, 382, 543, 689, 747, 846, 1260
PspPI GGNCC 6 cut(s) 381, 542, 688, 803, 992, 1241
PspPPI RGGWCCY 2 cut(s) 381, 542
PspXI VCTCGAGB 1 cut(s) 825
PstNI CAGNNNCTG 3 cut(s) 730, 1052, 1294
PsuI RGATCY 2 cut(s) 170, 894
RseI CAYNNNNRTG 1 cut(s) 650
SacI GAGCTC 1 cut(s) 832
SacII CCGCGG 1 cut(s) 493
SaqAI TTAA 1 cut(s) 914
SatI GCNGC 8 cut(s) 297, 453, 481, 490, 589, 1416, 1452, 1542
Sau3AI GATC 3 cut(s) 170, 894, 1588
Sau96I GGNCC 6 cut(s) 381, 542, 688, 803, 992, 1241
SchI GAGTC 1 cut(s) 1312
ScrFI CCNGG 3 cut(s) 197, 414, 523
SduI GDGCHC 3 cut(s) 748, 789, 832
SexAI ACCWGGT 1 cut(s) 195
SfaNI GCATC 4 cut(s) 52, 444, 1039, 1118
Sfr274I CTCGAG 1 cut(s) 825
Sfr303I CCGCGG 1 cut(s) 493
SfuI TTCGAA 1 cut(s) 50
SgrBI CCGCGG 1 cut(s) 493
SinI GGWCC 5 cut(s) 381, 542, 688, 803, 1241
SlaI CTCGAG 1 cut(s) 825
SmiMI CAYNNNNRTG 1 cut(s) 650
SmlI CTYRAG 4 cut(s) 20, 81, 825, 1164
SmoI CTYRAG 4 cut(s) 20, 81, 825, 1164
Sse9I AATT 8 cut(s) 5, 42, 126, 135, 439, 792, 1056, 1227
SsiI CCGC 6 cut(s) 378, 490, 492, 878, 1130, 1375
SspMI CTAG 5 cut(s) 98, 246, 567, 813, 1269
SstI GAGCTC 1 cut(s) 832
StyD4I CCNGG 3 cut(s) 195, 412, 521
StyI CCWWGG 2 cut(s) 848, 938
TaaI ACNGT 3 cut(s) 658, 1196, 1627
TaiI ACGT 1 cut(s) 182
TaqI TCGA 5 cut(s) 36, 50, 60, 826, 1587
TasI AATT 8 cut(s) 5, 42, 126, 135, 439, 792, 1056, 1227
TauI GCSGC 1 cut(s) 492
TfiI GAWTC 3 cut(s) 871, 1097, 1368
Tru1I TTAA 1 cut(s) 914
Tru9I TTAA 1 cut(s) 914
TscAI CASTG 3 cut(s) 481, 543, 637
TseFI GTSAC 2 cut(s) 1284, 1492
TseI GCWGC 7 cut(s) 296, 452, 480, 588, 1415, 1451, 1541
Tsp45I GTSAC 2 cut(s) 1284, 1492
TspDTI ATGAA 4 cut(s) 17, 54, 1205, 1583
TspGWI ACGGA 2 cut(s) 795, 959
TspRI CASTG 3 cut(s) 481, 543, 637
Van91I CCANNNNNTGG 2 cut(s) 939, 983
VpaK11BI GGWCC 5 cut(s) 381, 542, 688, 803, 1241
XapI RAATTY 4 cut(s) 5, 135, 792, 1227
XbaI TCTAGA 1 cut(s) 812
XcmI CCANNNNNNNNNTGG 1 cut(s) 1036
XhoI CTCGAG 1 cut(s) 825
XspI CTAG 5 cut(s) 98, 246, 567, 813, 1269
Zsp2I ATGCAT 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.