MD17G1056600.v1.1

Domain of unknown function (DUF4336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0015474
Physical Location & Seq
Forward (+)
4474978 .. 4478548
3571 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1056600.v1.1.491

Sequence Viewer

Length: 1437 bp
ATGACTCTGCATTATGTTATGTACCACTCCTCTCTTGGTGGTGCTGAAGACAAAGTACTAACAGAAGGTTTCAAAAAAGCAATGGCAGCCGTTGCCGTTTCTTCTCCAAAATCCTCCATTTTTCAGAACCCAATCTCTCGCAGGGAGCCAAGTTCCGGTCTTTTTGGCGGGTCCTTGAAGGGTTTTTGTTTTCAGTTGAGACCCGGAAACAAGAGCAGAGATCGCATCAATTTGGTGGTTGCTGCCACAAACTCCACCACCATCAGTGGCAGCAGAGGCCGCGGCGGCGACCGGTTTTACATCAACTTCACCGGGTTTCCTTTTCCACTGGGTCCTTTTCTCAACAGAAGCACTACTAGGACTGAGGCTGTGAAAGGCTGCATATGGTTATTTGAGCAAGAGCAAGCATTAGGCTTCAGCAGTGTGTCAACCAACATTAGAATGACAGTCATCAAACTAAAATCCGGAGGATTGTGGGTCCATGCGCCCATCGCTCCGACCAAAGAGTGTATTCAGCTTCTGAAAGAGTTAGGGGCTCCCGTAGAATACATAGTTCTGCCTACATTTGCTTATGAGCACAAAATTTTTGTTGGTCCGTTCTCTAGAGAGTTCCCTCGAGCTCAGGTATGGGTGGCACCAAGGCAATGGAGTTGGCCTTTGAATCTCCCGCTGGAGTTTTTTGGGATCTTTCGTGCTAAAACCTTAAAAGACGAGGATTTTTCCACTCCATGGGCTGATGAGATTGAGCAGAAAGTTCTCAGTTCACCAGAAGTTGGTATCGGGCCATATGTGGAGGTAGCTTTCTATCATAAGCGTTCCAGAACTCTACTGGTAACCGATGCTGTAATTTTTGTGCCAAGACAGCCACCTGATTGTATCAGCAAAGAATCCTTGTTGGCATCTGCAAAGAATGGCTTGGCGGTAAAACTTCTTAGTAAAGGAAAAGAAGTATCTCAAGAACCAGTAGTTGACAACAAGATGAACCGTCAAAAAGGATGGGAAAGAATGGTTCTTCAAATTTTGTTTCTTGGTCCATCAAATCTGTTGGAGCCGAATGCTAGCTTTGCTCAGATGTCACAGAAGCTGATTGTTTCACCCATTGTAAAGACTCTTGTCTTCAGCAAAGTTCCTGAAAAGGTCAGGGATTGGGTTGATGGAATCGTGCAGGACTGGAGGTTCAAGAGAATAATCCCTGCTCATTTTGCAGCACCAATCAACGCAGGCAGGTCTGATTTGTTAGCTGCATTTGCCTTTCTGGATGACCTTCTGGATGAGCCTTATGTCACTCGCCCATCACTCTCTCTTCTCTTCACATCACTATTGGGAAAGGCCGCGAGCTACTTCCCTCCCGATGACATGAAGACTTTATCATCCCTCGATCAGTTTTTGGTCTCGGTTGGAGCTGTGAAAAAGACCGTCTCAGGGCGCAAACGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4336

Protein Analysis

479

Amino Acids

53.17

Weight (kDa)

9.63

Isoelectric Point (pI)

44.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4336 PF14234 125 - 348 4.3e-85 Domain of unknown function (DUF4336)
DUF4336 PF14234 352 - 418 4.1e-18 Domain of unknown function (DUF4336)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01060 AT3G01060
fragaria_vesca FvH4_6g47510
malus_domestica MD17G1056600.v1.1
prunus_persica Prupe.3G258900_v2.0.a1
pyrus_communis pycom17g05560
rosa_chinensis RchiOBHm_Chr2g0166541
rosa_laevigata RLG00000021628
rosa_multiflora Rmu_sc0010842.1_g000007
rosa_roxburghii Rroxscaffold_2G00084760
rosa_rugosa Rorug02G0524100
rosa_samantha Rh2AG590000 Rh2BG602100 Rh2CG572800 Rh2DG612700
rosa_wichuraiana Rw2G049180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1215
AccB1I GGYRCC 1 cut(s) 634
AccB7I CCANNNNNTGG 2 cut(s) 729, 773
AccII CGCG 2 cut(s) 282, 1334
AccIII TCCGGA 1 cut(s) 464
AciI CCGC 7 cut(s) 168, 280, 282, 285, 668, 920, 1332
AclWI GGATC 1 cut(s) 692
AcsI RAATTY 2 cut(s) 582, 1017
AcuI CTGAAG 3 cut(s) 66, 400, 1102
AfaI GTAC 2 cut(s) 23, 57
AfiI CCNNNNNNNGG 4 cut(s) 155, 729, 773, 1422
AgeI ACCGGT 1 cut(s) 291
AgsI TTSAA 5 cut(s) 73, 178, 661, 1016, 1180
AloI GAACNNNNNNTCC 2 cut(s) 1160, 1192
AluBI AGCT 8 cut(s) 517, 620, 800, 1062, 1084, 1241, 1338, 1403
AluI AGCT 8 cut(s) 517, 620, 800, 1062, 1084, 1241, 1338, 1403
Alw21I GWGCWC 2 cut(s) 579, 622
Alw26I GTCTC 3 cut(s) 193, 1396, 1423
AlwI GGATC 1 cut(s) 692
AlwNI CAGNNNCTG 2 cut(s) 520, 1084
Ama87I CYCGRG 1 cut(s) 615
Aor13HI TCCGGA 1 cut(s) 464
AoxI GGCC 4 cut(s) 277, 653, 782, 1329
ApeKI GCWGC 6 cut(s) 86, 242, 270, 378, 1205, 1241
ApoI RAATTY 2 cut(s) 582, 1017
AsiGI ACCGGT 1 cut(s) 291
AspLEI GCGC 2 cut(s) 487, 1428
AspS9I GGNCC 6 cut(s) 171, 332, 478, 593, 782, 1031
AsuC2I CCSGG 2 cut(s) 204, 313
AsuHPI GGTGA 3 cut(s) 301, 756, 1086
AsuNHI GCTAGC 1 cut(s) 1058
AvaI CYCGRG 1 cut(s) 615
AvaII GGWCC 5 cut(s) 171, 332, 478, 593, 1031
BanI GGYRCC 1 cut(s) 634
BanII GRGCYC 2 cut(s) 538, 622
BarI GAAGNNNNNNTAC 2 cut(s) 39, 71
BbsI GAAGAC 3 cut(s) 54, 1108, 1367
Bbv12I GWGCWC 2 cut(s) 579, 622
BbvI GCAGC 6 cut(s) 98, 229, 282, 365, 1217, 1228
BccI CCATC 6 cut(s) 269, 497, 990, 1042, 1148, 1300
BceAI ACGGC 2 cut(s) 74, 80
BcnI CCSGG 2 cut(s) 204, 313
BcoDI GTCTC 3 cut(s) 193, 1396, 1423
BfaI CTAG 3 cut(s) 357, 603, 1059
BfuAI ACCTGC 1 cut(s) 1215
BglI GCCNNNNNGGC 1 cut(s) 285
BmcAI AGTACT 1 cut(s) 57
Bme1390I CCNGG 2 cut(s) 204, 313
Bme18I GGWCC 5 cut(s) 171, 332, 478, 593, 1031
BmeT110I CYCGRG 1 cut(s) 615
BmgT120I GGNCC 6 cut(s) 171, 332, 478, 593, 782, 1031
BmiI GGNNCC 7 cut(s) 147, 172, 333, 479, 537, 636, 1050
BmrFI CCNGG 2 cut(s) 204, 313
BmrI ACTGGG 1 cut(s) 338
BmsI GCATC 3 cut(s) 234, 829, 908
BmtI GCTAGC 1 cut(s) 1062
BmuI ACTGGG 1 cut(s) 338
BoxI GACNNNNGTC 1 cut(s) 1112
BpiI GAAGAC 3 cut(s) 54, 1108, 1367
BpmI CTGGAG 2 cut(s) 692, 1192
Bpu10I CCTNAGC 1 cut(s) 621
BpuEI CTTGAG 1 cut(s) 939
BpuMI CCSGG 2 cut(s) 204, 313
BsaI GGTCTC 2 cut(s) 193, 1396
BsaJI CCNNGG 3 cut(s) 280, 638, 728
BsaWI WCCGGW 3 cut(s) 155, 291, 464
Bsc4I CCNNNNNNNGG 4 cut(s) 155, 729, 773, 1422
Bse118I RCCGGY 1 cut(s) 291
Bse1I ACTGG 4 cut(s) 333, 834, 962, 1175
Bse3DI GCAATG 2 cut(s) 87, 650
BseAI TCCGGA 1 cut(s) 464
BseDI CCNNGG 3 cut(s) 280, 638, 728
BseGI GGATG 4 cut(s) 1001, 1264, 1276, 1370
BseLI CCNNNNNNNGG 4 cut(s) 155, 729, 773, 1422
BseMI GCAATG 2 cut(s) 87, 650
BseMII CTCAG 5 cut(s) 354, 635, 772, 1082, 1434
BseNI ACTGG 4 cut(s) 333, 834, 962, 1175
BseRI GAGGAG 1 cut(s) 19
BseXI GCAGC 6 cut(s) 98, 229, 282, 365, 1217, 1228
BsgI GTGCAG 1 cut(s) 1184
Bsh1236I CGCG 2 cut(s) 282, 1334
Bsh1285I CGRYCG 1 cut(s) 292
BshFI GGCC 4 cut(s) 279, 655, 784, 1331
BshNI GGYRCC 1 cut(s) 634
BshTI ACCGGT 1 cut(s) 291
BsiEI CGRYCG 1 cut(s) 292
BsiHKAI GWGCWC 2 cut(s) 579, 622
BsiHKCI CYCGRG 1 cut(s) 615
BsiSI CCGG 5 cut(s) 156, 204, 292, 312, 465
BslI CCNNNNNNNGG 4 cut(s) 155, 729, 773, 1422
BsmAI GTCTC 3 cut(s) 193, 1396, 1423
BsmBI CGTCTC 1 cut(s) 1423
BsmI GAATGC 1 cut(s) 1060
BsnI GGCC 4 cut(s) 279, 655, 784, 1331
Bso31I GGTCTC 2 cut(s) 193, 1396
BsoBI CYCGRG 1 cut(s) 615
Bsp1286I GDGCHC 3 cut(s) 538, 579, 622
Bsp13I TCCGGA 1 cut(s) 464
Bsp143I GATC 3 cut(s) 220, 684, 1378
Bsp19I CCATGG 1 cut(s) 728
BspACI CCGC 7 cut(s) 168, 280, 282, 285, 668, 920, 1332
BspANI GGCC 4 cut(s) 279, 655, 784, 1331
BspCNI CTCAG 5 cut(s) 355, 634, 771, 1081, 1433
BspEI TCCGGA 1 cut(s) 464
BspFNI CGCG 2 cut(s) 282, 1334
BspLI GGNNCC 7 cut(s) 147, 172, 333, 479, 537, 636, 1050
BspMI ACCTGC 1 cut(s) 1215
BspOI GCTAGC 1 cut(s) 1062
BspPI GGATC 1 cut(s) 692
BspT107I GGYRCC 1 cut(s) 634
BspTNI GGTCTC 2 cut(s) 193, 1396
BsrDI GCAATG 2 cut(s) 87, 650
BsrFI RCCGGY 1 cut(s) 291
BsrI ACTGG 4 cut(s) 333, 834, 962, 1175
BssAI RCCGGY 1 cut(s) 291
BssECI CCNNGG 3 cut(s) 280, 638, 728
BssMI GATC 3 cut(s) 220, 684, 1378
BssT1I CCWWGG 2 cut(s) 638, 728
Bst4CI ACNGT 3 cut(s) 448, 986, 1417
Bst6I CTCTTC 2 cut(s) 1308, 1313
BstAPI GCANNNNNTGC 1 cut(s) 92
BstC8I GCNNGC 4 cut(s) 405, 1060, 1222, 1336
BstDEI CTNAG 6 cut(s) 363, 621, 758, 932, 1068, 1420
BstDSI CCRYGG 2 cut(s) 280, 728
BstEII GGTNACC 1 cut(s) 832
BstF5I GGATG 4 cut(s) 1001, 1264, 1276, 1370
BstFNI CGCG 2 cut(s) 282, 1334
BstHHI GCGC 2 cut(s) 487, 1428
BstKTI GATC 3 cut(s) 223, 687, 1381
BstMAI GTCTC 3 cut(s) 193, 1396, 1423
BstMBI GATC 3 cut(s) 220, 684, 1378
BstMCI CGRYCG 1 cut(s) 292
BstPAI GACNNNNGTC 1 cut(s) 1112
BstPI GGTNACC 1 cut(s) 832
BstSCI CCNGG 2 cut(s) 202, 311
BstUI CGCG 2 cut(s) 282, 1334
BstV1I GCAGC 6 cut(s) 98, 229, 282, 365, 1217, 1228
BstV2I GAAGAC 3 cut(s) 54, 1108, 1367
BstX2I RGATCY 1 cut(s) 684
BstXI CCANNNNNNTGG 1 cut(s) 645
BstYI RGATCY 1 cut(s) 684
BsuRI GGCC 4 cut(s) 279, 655, 784, 1331
BtgI CCRYGG 2 cut(s) 280, 728
BtgZI GCGATG 1 cut(s) 475
BtsCI GGATG 4 cut(s) 1001, 1264, 1276, 1370
BtsI GCAGTG 1 cut(s) 427
BtsIMutI CAGTG 3 cut(s) 271, 326, 427
BveI ACCTGC 1 cut(s) 1215
Cac8I GCNNGC 4 cut(s) 405, 1060, 1222, 1336
CaiI CAGNNNCTG 2 cut(s) 520, 1084
CfoI GCGC 2 cut(s) 487, 1428
Cfr10I RCCGGY 1 cut(s) 291
Cfr13I GGNCC 6 cut(s) 171, 332, 478, 593, 782, 1031
Cfr42I CCGCGG 1 cut(s) 283
Csp6I GTAC 2 cut(s) 22, 56
CspAI ACCGGT 1 cut(s) 291
CviAII CATG 3 cut(s) 482, 729, 1357
CviQI GTAC 2 cut(s) 22, 56
DdeI CTNAG 6 cut(s) 363, 621, 758, 932, 1068, 1420
DpnI GATC 3 cut(s) 222, 686, 1380
DpnII GATC 3 cut(s) 220, 684, 1378
Eam1104I CTCTTC 2 cut(s) 1308, 1313
EarI CTCTTC 2 cut(s) 1308, 1313
Ecl136II GAGCTC 1 cut(s) 620
Eco130I CCWWGG 2 cut(s) 638, 728
Eco24I GRGCYC 2 cut(s) 538, 622
Eco31I GGTCTC 2 cut(s) 193, 1396
Eco47I GGWCC 5 cut(s) 171, 332, 478, 593, 1031
Eco53kI GAGCTC 1 cut(s) 620
Eco57I CTGAAG 3 cut(s) 66, 400, 1102
Eco88I CYCGRG 1 cut(s) 615
Eco91I GGTNACC 1 cut(s) 832
EcoICRI GAGCTC 1 cut(s) 620
EcoO109I RGGNCCY 2 cut(s) 171, 332
EcoO65I GGTNACC 1 cut(s) 832
EcoT14I CCWWGG 2 cut(s) 638, 728
EcoT38I GRGCYC 2 cut(s) 538, 622
ErhI CCWWGG 2 cut(s) 638, 728
Esp3I CGTCTC 1 cut(s) 1423
FaeI CATG 3 cut(s) 485, 732, 1360
FalI AAGNNNNNCTT 2 cut(s) 899, 931
FatI CATG 3 cut(s) 481, 728, 1356
FauI CCCGC 2 cut(s) 161, 675
FauNDI CATATG 2 cut(s) 383, 787
FokI GGATG 4 cut(s) 1008, 1271, 1283, 1357
FriOI GRGCYC 2 cut(s) 538, 622
FspBI CTAG 3 cut(s) 357, 603, 1059
GlaI GCGC 2 cut(s) 486, 1427
GsuI CTGGAG 2 cut(s) 692, 1192
HaeIII GGCC 4 cut(s) 279, 655, 784, 1331
HapII CCGG 5 cut(s) 156, 204, 292, 312, 465
HhaI GCGC 2 cut(s) 487, 1428
Hin1II CATG 3 cut(s) 485, 732, 1360
Hin6I GCGC 2 cut(s) 485, 1426
HinP1I GCGC 2 cut(s) 485, 1426
HincII GTYRAC 2 cut(s) 429, 970
HindII GTYRAC 2 cut(s) 429, 970
HinfI GANTC 5 cut(s) 4, 661, 887, 1108, 1158
HpaII CCGG 5 cut(s) 156, 204, 292, 312, 465
HphI GGTGA 3 cut(s) 301, 756, 1086
Hpy166II GTNNAC 3 cut(s) 429, 764, 970
Hpy188I TCNGA 5 cut(s) 126, 498, 522, 1071, 1231
Hpy188III TCNNGA 9 cut(s) 465, 603, 819, 956, 1130, 1180, 1256, 1268, 1349
Hpy8I GTNNAC 3 cut(s) 429, 764, 970
HpyAV CCTTC 3 cut(s) 59, 172, 1274
HpyCH4III ACNGT 3 cut(s) 448, 986, 1417
HpyCH4V TGCA 6 cut(s) 10, 381, 905, 1165, 1205, 1244
HpyF3I CTNAG 6 cut(s) 363, 621, 758, 932, 1068, 1420
Hsp92II CATG 3 cut(s) 485, 732, 1360
HspAI GCGC 2 cut(s) 485, 1426
Kpn2I TCCGGA 1 cut(s) 464
KspI CCGCGG 1 cut(s) 283
Kzo9I GATC 3 cut(s) 220, 684, 1378
LmnI GCTCC 5 cut(s) 145, 499, 541, 1048, 1400
Lsp1109I GCAGC 6 cut(s) 98, 229, 282, 365, 1217, 1228
LweI GCATC 3 cut(s) 234, 829, 908
MaeI CTAG 3 cut(s) 357, 603, 1059
MaeIII GTNAC 3 cut(s) 832, 1074, 1282
MalI GATC 3 cut(s) 222, 686, 1380
MboI GATC 3 cut(s) 220, 684, 1378
MboII GAAGA 7 cut(s) 59, 93, 1004, 1108, 1295, 1300, 1372
MflI RGATCY 1 cut(s) 684
MhlI GDGCHC 3 cut(s) 538, 579, 622
MluCI AATT 4 cut(s) 229, 582, 846, 1017
MlyI GAGTC 1 cut(s) 1102
MmeI TCCRAC 3 cut(s) 521, 1026, 1378
MroI TCCGGA 1 cut(s) 464
MseI TTAA 1 cut(s) 704
MslI CAYNNNNRTG 1 cut(s) 440
MspA1I CMGCKG 2 cut(s) 282, 670
MspI CCGG 5 cut(s) 156, 204, 292, 312, 465
MspR9I CCNGG 2 cut(s) 204, 313
Mva1269I GAATGC 1 cut(s) 1060
MvnI CGCG 2 cut(s) 282, 1334
NciI CCSGG 2 cut(s) 204, 313
NcoI CCATGG 1 cut(s) 728
NdeI CATATG 2 cut(s) 383, 787
NdeII GATC 3 cut(s) 220, 684, 1378
NheI GCTAGC 1 cut(s) 1058
NlaIII CATG 3 cut(s) 485, 732, 1360
NlaIV GGNNCC 7 cut(s) 147, 172, 333, 479, 537, 636, 1050
NmuCI GTSAC 2 cut(s) 1074, 1282
PaeR7I CTCGAG 1 cut(s) 615
PctI GAATGC 1 cut(s) 1060
PfeI GAWTC 3 cut(s) 661, 887, 1158
PflMI CCANNNNNTGG 2 cut(s) 729, 773
PinAI ACCGGT 1 cut(s) 291
PleI GAGTC 1 cut(s) 1102
PpsI GAGTC 1 cut(s) 1102
PpuMI RGGWCCY 2 cut(s) 171, 332
PshAI GACNNNNGTC 1 cut(s) 1112
Psp124BI GAGCTC 1 cut(s) 622
Psp5II RGGWCCY 2 cut(s) 171, 332
PspEI GGTNACC 1 cut(s) 832
PspN4I GGNNCC 7 cut(s) 147, 172, 333, 479, 537, 636, 1050
PspPI GGNCC 6 cut(s) 171, 332, 478, 593, 782, 1031
PspPPI RGGWCCY 2 cut(s) 171, 332
PspXI VCTCGAGB 1 cut(s) 615
PstNI CAGNNNCTG 2 cut(s) 520, 1084
PsuI RGATCY 1 cut(s) 684
RsaI GTAC 2 cut(s) 23, 57
RsaNI GTAC 2 cut(s) 22, 56
RseI CAYNNNNRTG 1 cut(s) 440
SacI GAGCTC 1 cut(s) 622
SacII CCGCGG 1 cut(s) 283
SaqAI TTAA 1 cut(s) 704
Sau3AI GATC 3 cut(s) 220, 684, 1378
Sau96I GGNCC 6 cut(s) 171, 332, 478, 593, 782, 1031
ScaI AGTACT 1 cut(s) 57
SchI GAGTC 1 cut(s) 1102
ScrFI CCNGG 2 cut(s) 204, 313
SduI GDGCHC 3 cut(s) 538, 579, 622
SfaNI GCATC 3 cut(s) 234, 829, 908
Sfr274I CTCGAG 1 cut(s) 615
Sfr303I CCGCGG 1 cut(s) 283
SgrBI CCGCGG 1 cut(s) 283
SinI GGWCC 5 cut(s) 171, 332, 478, 593, 1031
SlaI CTCGAG 1 cut(s) 615
SmiMI CAYNNNNRTG 1 cut(s) 440
SmlI CTYRAG 2 cut(s) 615, 954
SmoI CTYRAG 2 cut(s) 615, 954
Sse9I AATT 4 cut(s) 229, 582, 846, 1017
SsiI CCGC 7 cut(s) 168, 280, 282, 285, 668, 920, 1332
SspMI CTAG 3 cut(s) 357, 603, 1059
SstI GAGCTC 1 cut(s) 622
StyD4I CCNGG 2 cut(s) 202, 311
StyI CCWWGG 2 cut(s) 638, 728
TaaI ACNGT 3 cut(s) 448, 986, 1417
TaqI TCGA 2 cut(s) 616, 1377
TasI AATT 4 cut(s) 229, 582, 846, 1017
TatI WGTACW 1 cut(s) 55
TauI GCSGC 4 cut(s) 282, 285, 288, 1334
TfiI GAWTC 3 cut(s) 661, 887, 1158
Tru1I TTAA 1 cut(s) 704
Tru9I TTAA 1 cut(s) 704
TscAI CASTG 3 cut(s) 271, 333, 427
TseFI GTSAC 2 cut(s) 1074, 1282
TseI GCWGC 6 cut(s) 86, 242, 270, 378, 1205, 1241
Tsp45I GTSAC 2 cut(s) 1074, 1282
TspDTI ATGAA 2 cut(s) 995, 1373
TspGWI ACGGA 1 cut(s) 585
TspRI CASTG 3 cut(s) 271, 333, 427
Van91I CCANNNNNTGG 2 cut(s) 729, 773
VpaK11BI GGWCC 5 cut(s) 171, 332, 478, 593, 1031
XapI RAATTY 2 cut(s) 582, 1017
XbaI TCTAGA 1 cut(s) 602
XcmI CCANNNNNNNNNTGG 2 cut(s) 32, 826
XhoI CTCGAG 1 cut(s) 615
XspI CTAG 3 cut(s) 357, 603, 1059
ZrmI AGTACT 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.