AT3G12950

Peptidase, trypsin-like serine and cysteine proteases

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
Physical Location & Seq
Reverse (-)
4132491 .. 4135264
2774 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G12950.1

Sequence Viewer

Length: 1677 bp
ATGCCTCAGTATGGGTCAACGGGTCAACACTGCGAGTTCACGGCGGCGTCTTACTTCTCGTGGCCGACTTCAAGCAGGCTAAGCAACGCCGCGGAGGAAAGAGCTAACTATTTCTCAAACTTGCAGAAAGAGGAGGATGATGATGATGAGGTTTCTCCTGAACCAGTCTCCACAGAGCCTAAAGGGCAACGAGCCACTACTTTACTTGAGCTCATGACCATAAGAGCCTTCCACAGCAAGATGTTGCGGTGTTATAGTCTTGGAACAGCTATAGGGTTCAGGATTCGGAGAGGGGTTCTTACTGATATCCCAGCGATTATTGTGTTTGTGTCTCGGAAAGTGCATAAGCAATGGCTAAGTCCTCTTCAGTGTCTTCCTACTGCTCTTGAGGGAGCAGGAGGGATTTGGTGTGATGTGGATGTGGTGGAGTTTTCGTATTTTGGAGAACCTGATCATCAGCCCACACCTAAACAAACATTCACTACTGATATTGTTGATCATCTCCAAGGGTCAGATCCTTTCATTGGATCAGGCTCTCAGGTGGCAAGCCAAGAGACTTGTGGGACTTTGGGGGCAATAGTTAGGAGTCAGACTGGAGGTAGACAAGTTGGGTTTGTGACTAACCGCCATGTTGCTGTTAACTTAGATTACCCTAGCCAGAAGATGTTTCATCCTCTCCCTCCTGCCCTTGGTCCTGGTGTCTACCTTGGAGCAGTTGAGAGAGCCACTTCCTTCATCACAGACGATCTCTGGTTTGGCATTTTTGCTGGCACCAATCCAGAGACGTTTGTGAGAGCAGACGGTGCATTCATCCCCTTTGCAGACGACTATGACCTGTCACGAGTCACAACGTCTGTCAAAGGTGGAGTAGGAGAGATTGGAGAGGTCAAAGCCATAGAGCTGCAGTCTCCAGTTGGTAGTTTAGTGGGTAAACAGGTGGTGAAAGTCGGGAGAAGCTCTGGCTTGACTACAGGAACTGTGCTGGCTTATGCACTGGAGTACAATGACGAGAGAGGAGTCTGCTTCCTCACTGATTTTCTTGTTGTCGGGGAGAACCATCGCAGTCCGTTTGATCTTGAGGGAGACAGCGGAAGCCTCATAGTGATGAAAGGAGAGGAGAAGGCAAGACCCATTGGGATCATATGGGGAGGCACTGGAAGCCGCGGGAGGCTGAAACTGAAGGTAGGGGAGTGTCCAGAGAGCTGGACCACTGGTGTAGATCTGGGAAGGCTTCTTACACATCTCCAGCTTGATCTCATCACCACCGATGAAGGGTTAAAAGCGGCAGTGCAAGAACAAAGAGCGGCCTCAACAACGGGGATGAGCTCAATGGTTGCAGACTCATCACCTCCGTATGTGAATCTCAAGAAAGAGAAGAGAAGCCCAGAAGAAAAGTTAGAGGCATCATTAGGACCTCTTCAGGTCCAACATATTGATCTTGAAGAGAGAATAGAAACAAAAGGAGGTGCTCCAAGTGTCGAACACCAGTTCATGCCCACCTTCAGCGGTCAATGTTCCGCTTCTGCTTGGCCTGAGACTGCTAGAGAGGATCTTGTTGCCGGCTTTACAAATGGTAGCTGCGATGGGGATTTATGTGTTGGACTACGTTTAGGAGACGATGGAGCTAAACGCAGACGCACTCAGGTGACCAACGAAAGAATGAGACCCGCCGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

558

Amino Acids

60.53

Weight (kDa)

5.19

Isoelectric Point (pI)

46.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAL1_N PF25608 63 - 142 6.1e-43 NARROW LEAF 1 N-terminal domain
Nal1_C PF25819 157 - 424 2.4e-149 Nal1 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 770
AccBSI CCGCTC 1 cut(s) 1304
AccI GTMKAC 2 cut(s) 601, 702
AccII CGCG 2 cut(s) 92, 1164
AclWI GGATC 4 cut(s) 509, 535, 1145, 1557
AcoI YGGCCR 1 cut(s) 62
AcuI CTGAAG 4 cut(s) 350, 1199, 1403, 1486
AcyI GRCGYC 1 cut(s) 47
AfaI GTAC 1 cut(s) 1001
AfiI CCNNNNNNNGG 4 cut(s) 11, 524, 689, 1272
AgsI TTSAA 2 cut(s) 72, 1442
AjnI CCWGG 1 cut(s) 694
AleI CACNNNNGTG 1 cut(s) 1643
Alw21I GWGCWC 3 cut(s) 213, 1328, 1471
Alw26I GTCTC 9 cut(s) 172, 336, 548, 776, 912, 1077, 1529, 1608, 1657
AlwI GGATC 4 cut(s) 509, 535, 1145, 1557
AlwNI CAGNNNCTG 1 cut(s) 977
AoxI GGCC 3 cut(s) 62, 1305, 1529
ApeKI GCWGC 2 cut(s) 901, 1578
AspS9I GGNCC 4 cut(s) 692, 1206, 1412, 1423
AsuHPI GGTGA 4 cut(s) 952, 1252, 1338, 1657
AvaII GGWCC 4 cut(s) 692, 1206, 1412, 1423
BanI GGYRCC 1 cut(s) 770
BanII GRGCYC 2 cut(s) 213, 1328
BarI GAAGNNNNNNTAC 2 cut(s) 1219, 1251
BauI CACGAG 2 cut(s) 58, 840
BbsI GAAGAC 1 cut(s) 365
Bbv12I GWGCWC 3 cut(s) 213, 1328, 1471
BbvI GCAGC 2 cut(s) 888, 1565
BccI CCATC 3 cut(s) 1065, 1577, 1613
BceAI ACGGC 1 cut(s) 57
BciT130I CCWGG 1 cut(s) 696
BclI TGATCA 2 cut(s) 451, 496
BcoDI GTCTC 9 cut(s) 172, 336, 548, 776, 912, 1077, 1529, 1608, 1657
BfaI CTAG 2 cut(s) 654, 1542
BfmI CTRYAG 3 cut(s) 270, 902, 969
BglI GCCNNNNNGGC 1 cut(s) 184
BglII AGATCT 1 cut(s) 1219
BisI GCNGC 7 cut(s) 45, 90, 902, 1162, 1284, 1305, 1579
BlpI GCTNAGC 1 cut(s) 80
BlsI GCNGC 7 cut(s) 46, 91, 903, 1163, 1285, 1306, 1580
Bme1390I CCNGG 1 cut(s) 696
Bme18I GGWCC 4 cut(s) 692, 1206, 1412, 1423
BmgT120I GGNCC 4 cut(s) 692, 1206, 1412, 1423
BmiI GGNNCC 1 cut(s) 772
BmrFI CCNGG 1 cut(s) 696
BmsI GCATC 1 cut(s) 1412
BpiI GAAGAC 1 cut(s) 365
BpmI CTGGAG 4 cut(s) 615, 894, 1016, 1229
Bpu1102I GCTNAGC 1 cut(s) 80
BpuEI CTTGAG 4 cut(s) 227, 407, 1097, 1349
BsaHI GRCGYC 1 cut(s) 47
BsaI GGTCTC 1 cut(s) 1657
BsaJI CCNNGG 5 cut(s) 90, 505, 688, 706, 1162
BsaXI ACNNNNNCTCC 2 cut(s) 419, 449
Bsc4I CCNNNNNNNGG 4 cut(s) 11, 524, 689, 1272
Bse118I RCCGGY 1 cut(s) 1559
Bse1I ACTGG 7 cut(s) 164, 598, 911, 999, 1159, 1216, 1486
Bse3DI GCAATG 1 cut(s) 356
BseBI CCWGG 1 cut(s) 696
BseDI CCNNGG 5 cut(s) 90, 505, 688, 706, 1162
BseGI GGATG 5 cut(s) 142, 424, 670, 810, 1326
BseLI CCNNNNNNNGG 4 cut(s) 11, 524, 689, 1272
BseMI GCAATG 1 cut(s) 356
BseMII CTCAG 4 cut(s) 20, 551, 1524, 1655
BseNI ACTGG 7 cut(s) 164, 598, 911, 999, 1159, 1216, 1486
BseRI GAGGAG 3 cut(s) 146, 1029, 1130
BseXI GCAGC 2 cut(s) 888, 1565
BseYI CCCAGC 1 cut(s) 310
Bsh1236I CGCG 2 cut(s) 92, 1164
BshFI GGCC 3 cut(s) 64, 1307, 1531
BshNI GGYRCC 1 cut(s) 770
BsiHKAI GWGCWC 3 cut(s) 213, 1328, 1471
BsiSI CCGG 1 cut(s) 1560
BslFI GGGAC 1 cut(s) 577
BslI CCNNNNNNNGG 4 cut(s) 11, 524, 689, 1272
BsmAI GTCTC 9 cut(s) 172, 336, 548, 776, 912, 1077, 1529, 1608, 1657
BsmBI CGTCTC 2 cut(s) 776, 1608
BsmFI GGGAC 1 cut(s) 577
BsmI GAATGC 1 cut(s) 806
BsnI GGCC 3 cut(s) 64, 1307, 1531
Bso31I GGTCTC 1 cut(s) 1657
Bsp1286I GDGCHC 3 cut(s) 213, 1328, 1471
Bsp1720I GCTNAGC 1 cut(s) 80
BspANI GGCC 3 cut(s) 64, 1307, 1531
BspCNI CTCAG 4 cut(s) 19, 550, 1525, 1654
BspFNI CGCG 2 cut(s) 92, 1164
BspHI TCATGA 1 cut(s) 213
BspLI GGNNCC 1 cut(s) 772
BspMAI CTGCAG 1 cut(s) 906
BspPI GGATC 4 cut(s) 509, 535, 1145, 1557
BspT107I GGYRCC 1 cut(s) 770
BspTNI GGTCTC 1 cut(s) 1657
BsrBI CCGCTC 1 cut(s) 1304
BsrDI GCAATG 1 cut(s) 356
BsrFI RCCGGY 1 cut(s) 1559
BsrI ACTGG 7 cut(s) 164, 598, 911, 999, 1159, 1216, 1486
BssAI RCCGGY 1 cut(s) 1559
BssECI CCNNGG 5 cut(s) 90, 505, 688, 706, 1162
BssNI GRCGYC 1 cut(s) 47
BssSI CACGAG 2 cut(s) 58, 840
BssT1I CCWWGG 3 cut(s) 505, 688, 706
Bst2BI CACGAG 2 cut(s) 58, 840
Bst2UI CCWGG 1 cut(s) 696
Bst4CI ACNGT 2 cut(s) 803, 979
Bst6I CTCTTC 4 cut(s) 369, 1370, 1422, 1437
BstACI GRCGYC 1 cut(s) 47
BstAPI GCANNNNNTGC 1 cut(s) 803
BstC8I GCNNGC 5 cut(s) 77, 547, 769, 984, 1561
BstDEI CTNAG 7 cut(s) 6, 80, 356, 537, 643, 1533, 1641
BstDSI CCRYGG 2 cut(s) 90, 1162
BstEII GGTNACC 1 cut(s) 1645
BstF5I GGATG 5 cut(s) 142, 424, 670, 810, 1326
BstFNI CGCG 2 cut(s) 92, 1164
BstMAI GTCTC 9 cut(s) 172, 336, 548, 776, 912, 1077, 1529, 1608, 1657
BstMWI GCNNNNNNNGC 4 cut(s) 81, 184, 803, 1158
BstNI CCWGG 1 cut(s) 696
BstPI GGTNACC 1 cut(s) 1645
BstSCI CCNGG 1 cut(s) 694
BstSFI CTRYAG 3 cut(s) 270, 902, 969
BstUI CGCG 2 cut(s) 92, 1164
BstV1I GCAGC 2 cut(s) 888, 1565
BstV2I GAAGAC 1 cut(s) 365
BstX2I RGATCY 3 cut(s) 514, 1219, 1549
BstXI CCANNNNNNTGG 1 cut(s) 1203
BstYI RGATCY 3 cut(s) 514, 1219, 1549
BsuRI GGCC 3 cut(s) 64, 1307, 1531
BtgI CCRYGG 2 cut(s) 90, 1162
BtgZI GCGATG 2 cut(s) 1043, 1596
BtsCI GGATG 5 cut(s) 142, 424, 670, 810, 1326
BtsI GCAGTG 2 cut(s) 28, 1293
BtsIMutI CAGTG 7 cut(s) 28, 374, 992, 1029, 1152, 1209, 1293
Cac8I GCNNGC 5 cut(s) 77, 547, 769, 984, 1561
CaiI CAGNNNCTG 1 cut(s) 977
CciI TCATGA 1 cut(s) 213
Cfr10I RCCGGY 1 cut(s) 1559
Cfr13I GGNCC 4 cut(s) 692, 1206, 1412, 1423
Cfr42I CCGCGG 2 cut(s) 93, 1165
CseI GACGC 2 cut(s) 36, 1644
Csp6I GTAC 1 cut(s) 1000
CviAII CATG 3 cut(s) 214, 629, 1492
CviQI GTAC 1 cut(s) 1000
DdeI CTNAG 7 cut(s) 6, 80, 356, 537, 643, 1533, 1641
EaeI YGGCCR 1 cut(s) 62
Eam1104I CTCTTC 4 cut(s) 369, 1370, 1422, 1437
EarI CTCTTC 4 cut(s) 369, 1370, 1422, 1437
Ecl136II GAGCTC 2 cut(s) 211, 1326
Eco130I CCWWGG 3 cut(s) 505, 688, 706
Eco24I GRGCYC 2 cut(s) 213, 1328
Eco31I GGTCTC 1 cut(s) 1657
Eco32I GATATC 1 cut(s) 307
Eco47I GGWCC 4 cut(s) 692, 1206, 1412, 1423
Eco53kI GAGCTC 2 cut(s) 211, 1326
Eco57I CTGAAG 4 cut(s) 350, 1199, 1403, 1486
Eco91I GGTNACC 1 cut(s) 1645
EcoICRI GAGCTC 2 cut(s) 211, 1326
EcoO109I RGGNCCY 1 cut(s) 1412
EcoO65I GGTNACC 1 cut(s) 1645
EcoRII CCWGG 1 cut(s) 694
EcoRV GATATC 1 cut(s) 307
EcoT14I CCWWGG 3 cut(s) 505, 688, 706
EcoT38I GRGCYC 2 cut(s) 213, 1328
ErhI CCWWGG 3 cut(s) 505, 688, 706
Esp3I CGTCTC 2 cut(s) 776, 1608
FaeI CATG 3 cut(s) 217, 632, 1495
FaqI GGGAC 1 cut(s) 577
FatI CATG 3 cut(s) 213, 628, 1491
FauI CCCGC 2 cut(s) 1157, 1675
FauNDI CATATG 1 cut(s) 1142
FbaI TGATCA 2 cut(s) 451, 496
FblI GTMKAC 2 cut(s) 601, 702
Fnu4HI GCNGC 7 cut(s) 45, 90, 902, 1162, 1284, 1305, 1579
FokI GGATG 5 cut(s) 149, 431, 657, 797, 1333
FriOI GRGCYC 2 cut(s) 213, 1328
Fsp4HI GCNGC 7 cut(s) 45, 90, 902, 1162, 1284, 1305, 1579
FspBI CTAG 2 cut(s) 654, 1542
GluI GCNGC 7 cut(s) 45, 90, 902, 1162, 1284, 1305, 1579
GsaI CCCAGC 1 cut(s) 314
GsuI CTGGAG 4 cut(s) 615, 894, 1016, 1229
HaeIII GGCC 3 cut(s) 64, 1307, 1531
HapII CCGG 1 cut(s) 1560
HgaI GACGC 2 cut(s) 36, 1644
Hin1I GRCGYC 1 cut(s) 47
Hin1II CATG 3 cut(s) 217, 632, 1495
HincII GTYRAC 3 cut(s) 18, 26, 640
HindII GTYRAC 3 cut(s) 18, 26, 640
HinfI GANTC 6 cut(s) 283, 586, 843, 1017, 1340, 1360
HpaI GTTAAC 1 cut(s) 640
HpaII CCGG 1 cut(s) 1560
HphI GGTGA 4 cut(s) 952, 1252, 1338, 1657
Hpy166II GTNNAC 7 cut(s) 18, 26, 39, 602, 640, 703, 932
Hpy188I TCNGA 4 cut(s) 288, 336, 514, 591
Hpy8I GTNNAC 7 cut(s) 18, 26, 39, 602, 640, 703, 932
HpyAV CCTTC 7 cut(s) 238, 742, 1114, 1174, 1221, 1265, 1510
HpyCH4III ACNGT 2 cut(s) 803, 979
HpyCH4IV ACGT 3 cut(s) 785, 851, 1606
HpyCH4V TGCA 8 cut(s) 124, 343, 806, 821, 904, 992, 1291, 1337
HpyF10VI GCNNNNNNNGC 4 cut(s) 81, 184, 803, 1158
HpyF3I CTNAG 7 cut(s) 6, 80, 356, 537, 643, 1533, 1641
HpySE526I ACGT 3 cut(s) 785, 851, 1606
Hsp92I GRCGYC 1 cut(s) 47
Hsp92II CATG 3 cut(s) 217, 632, 1495
KroI GCCGGC 1 cut(s) 1559
KroNI GCCGGC 1 cut(s) 1561
Ksp22I TGATCA 2 cut(s) 451, 496
KspAI GTTAAC 1 cut(s) 640
KspI CCGCGG 2 cut(s) 93, 1165
LmnI GCTCC 4 cut(s) 392, 710, 1474, 1622
Lsp1109I GCAGC 2 cut(s) 888, 1565
LweI GCATC 1 cut(s) 1412
MaeI CTAG 2 cut(s) 654, 1542
MaeII ACGT 3 cut(s) 785, 851, 1606
MaeIII GTNAC 4 cut(s) 616, 837, 844, 1645
MbiI CCGCTC 1 cut(s) 1304
MboII GAAGA 7 cut(s) 356, 365, 673, 1387, 1400, 1409, 1454
MflI RGATCY 3 cut(s) 514, 1219, 1549
MhlI GDGCHC 3 cut(s) 213, 1328, 1471
MlyI GAGTC 4 cut(s) 595, 852, 1026, 1334
MmeI TCCRAC 2 cut(s) 1450, 1579
MroNI GCCGGC 1 cut(s) 1559
MseI TTAA 2 cut(s) 639, 1277
MslI CAYNNNNRTG 2 cut(s) 1103, 1643
MspA1I CMGCKG 4 cut(s) 92, 1089, 1164, 1506
MspI CCGG 1 cut(s) 1560
MspR9I CCNGG 1 cut(s) 696
Mva1269I GAATGC 1 cut(s) 806
MvaI CCWGG 1 cut(s) 696
MvnI CGCG 2 cut(s) 92, 1164
MwoI GCNNNNNNNGC 4 cut(s) 81, 184, 803, 1158
NaeI GCCGGC 1 cut(s) 1561
NdeI CATATG 1 cut(s) 1142
NgoMIV GCCGGC 1 cut(s) 1559
NlaIII CATG 3 cut(s) 217, 632, 1495
NlaIV GGNNCC 1 cut(s) 772
NmuCI GTSAC 4 cut(s) 616, 837, 844, 1645
OliI CACNNNNGTG 1 cut(s) 1643
PagI TCATGA 1 cut(s) 213
PctI GAATGC 1 cut(s) 806
PdiI GCCGGC 1 cut(s) 1561
PfeI GAWTC 2 cut(s) 283, 1360
PkrI GCNGC 7 cut(s) 46, 91, 903, 1163, 1285, 1306, 1580
PleI GAGTC 4 cut(s) 594, 851, 1025, 1334
PpsI GAGTC 4 cut(s) 594, 851, 1025, 1334
PpuMI RGGWCCY 1 cut(s) 1412
Psp124BI GAGCTC 2 cut(s) 213, 1328
Psp5II RGGWCCY 1 cut(s) 1412
Psp6I CCWGG 1 cut(s) 694
PspEI GGTNACC 1 cut(s) 1645
PspFI CCCAGC 1 cut(s) 310
PspGI CCWGG 1 cut(s) 694
PspN4I GGNNCC 1 cut(s) 772
PspPI GGNCC 4 cut(s) 692, 1206, 1412, 1423
PspPPI RGGWCCY 1 cut(s) 1412
PstI CTGCAG 1 cut(s) 906
PstNI CAGNNNCTG 1 cut(s) 977
PsuI RGATCY 3 cut(s) 514, 1219, 1549
RsaI GTAC 1 cut(s) 1001
RsaNI GTAC 1 cut(s) 1000
RseI CAYNNNNRTG 2 cut(s) 1103, 1643
SacI GAGCTC 2 cut(s) 213, 1328
SacII CCGCGG 2 cut(s) 93, 1165
SaqAI TTAA 2 cut(s) 639, 1277
SatI GCNGC 7 cut(s) 45, 90, 902, 1162, 1284, 1305, 1579
Sau96I GGNCC 4 cut(s) 692, 1206, 1412, 1423
SchI GAGTC 4 cut(s) 595, 852, 1026, 1334
ScrFI CCNGG 1 cut(s) 696
SduI GDGCHC 3 cut(s) 213, 1328, 1471
SfaNI GCATC 1 cut(s) 1412
SfcI CTRYAG 3 cut(s) 270, 902, 969
Sfr303I CCGCGG 2 cut(s) 93, 1165
SgrBI CCGCGG 2 cut(s) 93, 1165
SinI GGWCC 4 cut(s) 692, 1206, 1412, 1423
SmiMI CAYNNNNRTG 2 cut(s) 1103, 1643
SmlI CTYRAG 4 cut(s) 206, 386, 1076, 1364
SmoI CTYRAG 4 cut(s) 206, 386, 1076, 1364
SspMI CTAG 2 cut(s) 654, 1542
SstI GAGCTC 2 cut(s) 213, 1328
StyD4I CCNGG 1 cut(s) 694
StyI CCWWGG 3 cut(s) 505, 688, 706
TaaI ACNGT 2 cut(s) 803, 979
TaiI ACGT 3 cut(s) 788, 854, 1609
TaqI TCGA 1 cut(s) 1479
TatI WGTACW 1 cut(s) 999
TauI GCSGC 5 cut(s) 47, 92, 1164, 1286, 1307
TfiI GAWTC 2 cut(s) 283, 1360
Tru1I TTAA 2 cut(s) 639, 1277
Tru9I TTAA 2 cut(s) 639, 1277
TscAI CASTG 7 cut(s) 35, 374, 999, 1036, 1159, 1216, 1293
TseFI GTSAC 4 cut(s) 616, 837, 844, 1645
TseI GCWGC 2 cut(s) 901, 1578
Tsp45I GTSAC 4 cut(s) 616, 837, 844, 1645
TspDTI ATGAA 7 cut(s) 511, 659, 724, 799, 1121, 1284, 1480
TspGWI ACGGA 2 cut(s) 1056, 1341
TspRI CASTG 7 cut(s) 35, 374, 999, 1036, 1159, 1216, 1293
VpaK11BI GGWCC 4 cut(s) 692, 1206, 1412, 1423
XcmI CCANNNNNNNNNTGG 1 cut(s) 557
XmiI GTMKAC 2 cut(s) 601, 702
XspI CTAG 2 cut(s) 654, 1542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.