AT3G44850

SRSF protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0015853
Physical Location & Seq
Reverse (-)
16374243 .. 16377263
3021 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G44850.1

Sequence Viewer

Length: 1605 bp
ATGGCGGAGGACAAAAACAACGGACGTGTGAATGACGAAAGTGAGTACTCGTCGGAGGACGAAGGAACAGAGGATTATAAGAAAGGAGGATACCATACAGTAAGAGTCGGTGATACTTTTAAGAATGGAGCTTATGTGATTCAGAGTAAACTTGGTTGGGGGCATTTCTCCACTGTTTGGCTTGCTTGGGATACGCAAGAATCGCGTTATGTAGCTTTGAAAGTTCAGAAGAGTGCTCAACATTATACAGAGGCAGCAATGGATGAGATAAAGATTTTGAAACAGATAGCTGAAGGTGACTCGGGTGATAAGAAGTGTGTTGTAAAGCTACTGGATCATTTCAAGCATACGGGTCCTAATGGGAAGCATGTTTGTATGGTTTTCGAGTATTTGGGAGATAACCTTTTGTCGGTTATTAAGTACAGTGATTACCGAGGGGTTCCTCTTCACATGGTTAAAGAGCTTTGTTTTCATATTTTGGTTGGGTTGGATTATCTTCATCGTGAGCTTTCTATTATTCATACTGATTTAAAGCCGGAGAATGTTCTGCTTTTGTCTACGATTGATCCGTCGAGAGATGTGAGGAGATCAGGTGTTCCACTTGTTCTGCCTATAACCAAGGACAAGATTGTTTCTGAGTCTGCTGTTAAACCTGAAACTAAAAGTTATACGTACAATGGTGATTTGACAAAGAATCAGAAGAAGAAGATTCGTAAGAAAGCGAAGAAGGTGGTGGCTCAAGATTTTGGTGGAGAGGAAGCTTTGGAAGAAAGTGAGAGAGATTCTAACTCCGAAGCAAGAATAAATGGCAATTCCACCGTAGAGAGATCTGAAGGAAGTTCCACAAGATTAATGGAAGGTGAAGAAGCCAGAGAGAAGGCTAACAAAAAGAATGGTCGGGGTAGTAGGAGAGGAAGCCGATCCACAAGGCAGAAATTACTGAGTGATATAGAGTGCAAATGCAAATTAGTAGATTTTGGGAATGCTTGTTGGACATATAAACAGTTCACAAGCGATATTCAGACAAGACAGTATCGATGTCCAGAGGTTGTTCTTGGCTCAAAATACTCAACTTCTGCAGATATGTGGTCCTTTGCTTGTATTTGTTTCGAGCTGGCCACCGGAGATGTCTTGTTCGATCCTCACAGTGGTGAAAATTATGACCGGGATGAGGATCACCTGGCATTGATGATGGAGCTTCTAGGGATGATGCCACGAAAGATTGCATTAGGTGGTCGATACTCACGAGATTTCTTCAACCGACAAGGTGAGCTAAGGCACATCCGGCGGTTGCGGTTTTGGCCCATCAGCAAAGTCTTGAAGGAGAAATATGATTTCAGCGAGCAGGACGCAAAAGACATGTCAGATTTCTTAGTCACAATTCTTGAATTTGTGCCTGAGAAGAGACCAACAGCCGCCCAATGCCTAAAACACCCATGGTTCAATCCTGGCCCGAGATTGCTTGAACCGTCACTGAAACCACAACAACCAAAAGGAGAAGAAGAAGGAGCAGCAAATGAGAACATAGAAAAGGAAAAGGACGAAAGAGAAGCAATGGAAGCTGGTGTAGGGAATATCGCTATTGATGTCTCTGAACCGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

534

Amino Acids

60.75

Weight (kDa)

6.44

Isoelectric Point (pI)

40.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 45 - 185 1.2e-19 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 47 - 185 4.3e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 316 - 481 3.6e-21 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015853)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44850 AT5G22840
fragaria_vesca FvH4_3g30170
malus_domestica MD03G1130900.v1.1 MD11G1153400.v1.1
prunus_persica Prupe.6G116000_v2.0.a1
pyrus_communis pycom11g12520
rosa_chinensis RchiOBHm_Chr5g0055961
rosa_laevigata RLG00000035037
rosa_multiflora Rmu_sc0000604.1_g000003 Rmu_sc0014108.1_g000001
rosa_roxburghii Rroxscaffold_1G00024340
rosa_rugosa Rorug05G0296400
rosa_samantha Rh5AG367800
rosa_wichuraiana Rw5G034500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 78
AccB7I CCANNNNNTGG 1 cut(s) 177
AccI GTMKAC 1 cut(s) 557
AccII CGCG 1 cut(s) 205
AciI CCGC 4 cut(s) 5, 1288, 1294, 1416
AclWI GGATC 5 cut(s) 342, 560, 915, 1133, 1182
AcoI YGGCCR 1 cut(s) 1116
AcsI RAATTY 1 cut(s) 1388
AcuI CTGAAG 2 cut(s) 312, 852
AfaI GTAC 3 cut(s) 47, 422, 674
AfiI CCNNNNNNNGG 4 cut(s) 177, 409, 1148, 1171
AflIII ACRYGT 2 cut(s) 25, 1359
AgsI TTSAA 8 cut(s) 220, 280, 343, 1258, 1321, 1388, 1444, 1466
AjiI CACGTC 1 cut(s) 26
AjnI CCWGG 2 cut(s) 1179, 1447
AleI CACNNNNGTG 1 cut(s) 1149
Alw21I GWGCWC 1 cut(s) 238
Alw26I GTCTC 2 cut(s) 1399, 1594
AlwI GGATC 5 cut(s) 342, 560, 915, 1133, 1182
Ama87I CYCGRG 2 cut(s) 301, 1453
AoxI GGCC 3 cut(s) 1116, 1301, 1450
ApeKI GCWGC 2 cut(s) 254, 1511
ApoI RAATTY 1 cut(s) 1388
ArsI GACNNNNNNTTYG 2 cut(s) 1346, 1378
AseI ATTAAT 1 cut(s) 851
AspS9I GGNCC 4 cut(s) 353, 1089, 1302, 1451
AsuC2I CCSGG 1 cut(s) 1166
AsuHPI GGTGA 8 cut(s) 122, 308, 317, 692, 872, 1163, 1169, 1280
AvaI CYCGRG 2 cut(s) 301, 1453
AvaII GGWCC 2 cut(s) 353, 1089
BalI TGGCCA 1 cut(s) 1118
BauI CACGAG 1 cut(s) 1245
Bbv12I GWGCWC 1 cut(s) 238
BbvI GCAGC 2 cut(s) 266, 1523
BccI CCATC 2 cut(s) 1186, 1313
BciT130I CCWGG 2 cut(s) 1181, 1449
BciVI GTATCC 2 cut(s) 83, 184
BcnI CCSGG 1 cut(s) 1166
BcoDI GTCTC 2 cut(s) 1399, 1594
BfaI CTAG 1 cut(s) 1202
BfmI CTRYAG 1 cut(s) 1077
BfuI GTATCC 2 cut(s) 83, 184
BglII AGATCT 1 cut(s) 827
BisI GCNGC 3 cut(s) 255, 1416, 1512
BlsI GCNGC 3 cut(s) 256, 1417, 1513
BmcAI AGTACT 1 cut(s) 47
Bme1390I CCNGG 3 cut(s) 1166, 1181, 1449
Bme18I GGWCC 2 cut(s) 353, 1089
BmeT110I CYCGRG 2 cut(s) 301, 1453
BmgBI CACGTC 1 cut(s) 26
BmgT120I GGNCC 4 cut(s) 353, 1089, 1302, 1451
BmiI GGNNCC 2 cut(s) 354, 441
BmrFI CCNGG 3 cut(s) 1166, 1181, 1449
BmsI GCATC 1 cut(s) 1200
Bpu10I CCTNAGC 1 cut(s) 1274
BpuEI CTTGAG 1 cut(s) 723
BpuMI CCSGG 1 cut(s) 1166
Bsa29I ATCGAT 1 cut(s) 1036
BsaAI YACGTR 1 cut(s) 672
BsaBI GATNNNNATC 1 cut(s) 1173
BsaI GGTCTC 1 cut(s) 1399
BsaJI CCNNGG 3 cut(s) 433, 618, 1436
BsaWI WCCGGW 1 cut(s) 1121
BsaXI ACNNNNNCTCC 4 cut(s) 120, 150, 577, 607
Bsc4I CCNNNNNNNGG 4 cut(s) 177, 409, 1148, 1171
Bse1I ACTGG 1 cut(s) 336
Bse3DI GCAATG 2 cut(s) 264, 1560
Bse8I GATNNNNATC 1 cut(s) 1173
BseBI CCWGG 2 cut(s) 1181, 1449
BseCI ATCGAT 1 cut(s) 1036
BseDI CCNNGG 3 cut(s) 433, 618, 1436
BseGI GGATG 4 cut(s) 268, 1174, 1212, 1281
BseJI GATNNNNATC 1 cut(s) 1173
BseLI CCNNNNNNNGG 4 cut(s) 177, 409, 1148, 1171
BseMI GCAATG 2 cut(s) 264, 1560
BseMII CTCAG 3 cut(s) 627, 932, 1389
BseNI ACTGG 1 cut(s) 336
BseRI GAGGAG 1 cut(s) 598
BseXI GCAGC 2 cut(s) 266, 1523
Bsh1236I CGCG 1 cut(s) 205
BshFI GGCC 3 cut(s) 1118, 1303, 1452
BshVI ATCGAT 1 cut(s) 1036
BsiHKAI GWGCWC 1 cut(s) 238
BsiHKCI CYCGRG 2 cut(s) 301, 1453
BsiSI CCGG 4 cut(s) 536, 1122, 1165, 1285
BslI CCNNNNNNNGG 4 cut(s) 177, 409, 1148, 1171
BsmAI GTCTC 2 cut(s) 1399, 1594
BsmI GAATGC 1 cut(s) 988
BsnI GGCC 3 cut(s) 1118, 1303, 1452
Bso31I GGTCTC 1 cut(s) 1399
BsoBI CYCGRG 2 cut(s) 301, 1453
Bsp1286I GDGCHC 1 cut(s) 238
Bsp143I GATC 7 cut(s) 334, 565, 587, 827, 920, 1138, 1174
Bsp19I CCATGG 1 cut(s) 1436
BspACI CCGC 4 cut(s) 5, 1288, 1294, 1416
BspANI GGCC 3 cut(s) 1118, 1303, 1452
BspCNI CTCAG 3 cut(s) 628, 933, 1390
BspDI ATCGAT 1 cut(s) 1036
BspFNI CGCG 1 cut(s) 205
BspLI GGNNCC 2 cut(s) 354, 441
BspMAI CTGCAG 1 cut(s) 1081
BspPI GGATC 5 cut(s) 342, 560, 915, 1133, 1182
BspTNI GGTCTC 1 cut(s) 1399
BsrDI GCAATG 2 cut(s) 264, 1560
BsrI ACTGG 1 cut(s) 336
BssECI CCNNGG 3 cut(s) 433, 618, 1436
BssMI GATC 7 cut(s) 334, 565, 587, 827, 920, 1138, 1174
BssSI CACGAG 1 cut(s) 1245
BssT1I CCWWGG 2 cut(s) 618, 1436
Bst2BI CACGAG 1 cut(s) 1245
Bst2UI CCWGG 2 cut(s) 1181, 1449
Bst4CI ACNGT 8 cut(s) 100, 175, 425, 820, 1005, 1032, 1148, 1470
Bst6I CTCTTC 3 cut(s) 224, 450, 1397
BstBAI YACGTR 1 cut(s) 672
BstC8I GCNNGC 3 cut(s) 183, 1116, 1343
BstDEI CTNAG 5 cut(s) 636, 941, 1274, 1372, 1398
BstDSI CCRYGG 1 cut(s) 1436
BstF5I GGATG 4 cut(s) 268, 1174, 1212, 1281
BstFNI CGCG 1 cut(s) 205
BstKTI GATC 7 cut(s) 337, 568, 590, 830, 923, 1141, 1177
BstMAI GTCTC 2 cut(s) 1399, 1594
BstMBI GATC 7 cut(s) 334, 565, 587, 827, 920, 1138, 1174
BstMWI GCNNNNNNNGC 4 cut(s) 202, 1285, 1300, 1559
BstNI CCWGG 2 cut(s) 1181, 1449
BstNSI RCATGY 2 cut(s) 371, 1363
BstSCI CCNGG 3 cut(s) 1164, 1179, 1447
BstSFI CTRYAG 1 cut(s) 1077
BstSNI TACGTA 1 cut(s) 672
BstUI CGCG 1 cut(s) 205
BstV1I GCAGC 2 cut(s) 266, 1523
BstX2I RGATCY 1 cut(s) 827
BstYI RGATCY 1 cut(s) 827
Bsu15I ATCGAT 1 cut(s) 1036
BsuI GTATCC 2 cut(s) 83, 184
BsuRI GGCC 3 cut(s) 1118, 1303, 1452
BsuTUI ATCGAT 1 cut(s) 1036
BtgI CCRYGG 1 cut(s) 1436
BtrI CACGTC 1 cut(s) 26
BtsCI GGATG 4 cut(s) 268, 1174, 1212, 1281
BtsIMutI CAGTG 4 cut(s) 171, 430, 1153, 1472
Cac8I GCNNGC 3 cut(s) 183, 1116, 1343
Cfr13I GGNCC 4 cut(s) 353, 1089, 1302, 1451
ClaI ATCGAT 1 cut(s) 1036
CseI GACGC 1 cut(s) 1358
Csp6I GTAC 3 cut(s) 46, 421, 673
CviAII CATG 4 cut(s) 368, 451, 1360, 1437
CviQI GTAC 3 cut(s) 46, 421, 673
DdeI CTNAG 5 cut(s) 636, 941, 1274, 1372, 1398
DpnI GATC 7 cut(s) 336, 567, 589, 829, 922, 1140, 1176
DpnII GATC 7 cut(s) 334, 565, 587, 827, 920, 1138, 1174
DraI TTTAAA 1 cut(s) 531
EaeI YGGCCR 1 cut(s) 1116
Eam1104I CTCTTC 3 cut(s) 224, 450, 1397
EarI CTCTTC 3 cut(s) 224, 450, 1397
EciI GGCGGA 1 cut(s) 20
Eco105I TACGTA 1 cut(s) 672
Eco130I CCWWGG 2 cut(s) 618, 1436
Eco31I GGTCTC 1 cut(s) 1399
Eco47I GGWCC 2 cut(s) 353, 1089
Eco57I CTGAAG 2 cut(s) 312, 852
Eco88I CYCGRG 2 cut(s) 301, 1453
EcoO109I RGGNCCY 1 cut(s) 353
EcoRII CCWGG 2 cut(s) 1179, 1447
EcoT14I CCWWGG 2 cut(s) 618, 1436
ErhI CCWWGG 2 cut(s) 618, 1436
FaeI CATG 4 cut(s) 371, 454, 1363, 1440
FatI CATG 4 cut(s) 367, 450, 1359, 1436
FblI GTMKAC 1 cut(s) 557
Fnu4HI GCNGC 3 cut(s) 255, 1416, 1512
FokI GGATG 4 cut(s) 275, 1181, 1219, 1268
Fsp4HI GCNGC 3 cut(s) 255, 1416, 1512
FspBI CTAG 1 cut(s) 1202
GluI GCNGC 3 cut(s) 255, 1416, 1512
HaeIII GGCC 3 cut(s) 1118, 1303, 1452
HapII CCGG 4 cut(s) 536, 1122, 1165, 1285
HgaI GACGC 1 cut(s) 1358
Hin1II CATG 4 cut(s) 371, 454, 1363, 1440
HindIII AAGCTT 1 cut(s) 759
HinfI GANTC 8 cut(s) 105, 139, 200, 299, 638, 694, 709, 782
HpaII CCGG 4 cut(s) 536, 1122, 1165, 1285
HphI GGTGA 8 cut(s) 122, 308, 317, 692, 872, 1163, 1169, 1280
Hpy166II GTNNAC 3 cut(s) 149, 558, 1008
Hpy188III TCNNGA 7 cut(s) 503, 573, 740, 1043, 1245, 1318, 1385
Hpy8I GTNNAC 3 cut(s) 149, 558, 1008
Hpy99I CGWCG 2 cut(s) 55, 574
HpyAV CCTTC 8 cut(s) 56, 287, 721, 827, 851, 871, 1315, 1499
HpyCH4III ACNGT 8 cut(s) 100, 175, 425, 820, 1005, 1032, 1148, 1470
HpyCH4IV ACGT 2 cut(s) 25, 671
HpyCH4V TGCA 4 cut(s) 957, 963, 1079, 1226
HpyF10VI GCNNNNNNNGC 4 cut(s) 202, 1285, 1300, 1559
HpyF3I CTNAG 5 cut(s) 636, 941, 1274, 1372, 1398
HpySE526I ACGT 2 cut(s) 25, 671
Hsp92II CATG 4 cut(s) 371, 454, 1363, 1440
Kzo9I GATC 7 cut(s) 334, 565, 587, 827, 920, 1138, 1174
LmnI GCTCC 3 cut(s) 128, 1195, 1508
Lsp1109I GCAGC 2 cut(s) 266, 1523
LweI GCATC 1 cut(s) 1200
MaeI CTAG 1 cut(s) 1202
MaeII ACGT 2 cut(s) 25, 671
MaeIII GTNAC 3 cut(s) 296, 1375, 1470
MalI GATC 7 cut(s) 336, 567, 589, 829, 922, 1140, 1176
MboI GATC 7 cut(s) 334, 565, 587, 827, 920, 1138, 1174
MflI RGATCY 1 cut(s) 827
MhlI GDGCHC 1 cut(s) 238
MlsI TGGCCA 1 cut(s) 1118
MluCI AATT 6 cut(s) 811, 935, 965, 1156, 1380, 1388
MluNI TGGCCA 1 cut(s) 1118
MlyI GAGTC 3 cut(s) 114, 293, 647
MmeI TCCRAC 3 cut(s) 33, 468, 971
Mox20I TGGCCA 1 cut(s) 1118
MscI TGGCCA 1 cut(s) 1118
MseI TTAA 6 cut(s) 120, 417, 456, 530, 648, 851
MslI CAYNNNNRTG 1 cut(s) 1149
Msp20I TGGCCA 1 cut(s) 1118
MspI CCGG 4 cut(s) 536, 1122, 1165, 1285
MspR9I CCNGG 3 cut(s) 1166, 1181, 1449
Mva1269I GAATGC 1 cut(s) 988
MvaI CCWGG 2 cut(s) 1181, 1449
MvnI CGCG 1 cut(s) 205
MwoI GCNNNNNNNGC 4 cut(s) 202, 1285, 1300, 1559
NciI CCSGG 1 cut(s) 1166
NcoI CCATGG 1 cut(s) 1436
NdeII GATC 7 cut(s) 334, 565, 587, 827, 920, 1138, 1174
NlaIII CATG 4 cut(s) 371, 454, 1363, 1440
NlaIV GGNNCC 2 cut(s) 354, 441
NmuCI GTSAC 3 cut(s) 296, 1375, 1470
NspI RCATGY 2 cut(s) 371, 1363
OliI CACNNNNGTG 1 cut(s) 1149
PciI ACATGT 1 cut(s) 1359
PcsI WCGNNNNNNNCGW 2 cut(s) 566, 1243
PctI GAATGC 1 cut(s) 988
PfeI GAWTC 5 cut(s) 139, 200, 694, 709, 782
PflMI CCANNNNNTGG 1 cut(s) 177
PkrI GCNGC 3 cut(s) 256, 1417, 1513
PleI GAGTC 3 cut(s) 113, 293, 646
PpsI GAGTC 3 cut(s) 113, 293, 646
Ppu21I YACGTR 1 cut(s) 672
PpuMI RGGWCCY 1 cut(s) 353
PscI ACATGT 1 cut(s) 1359
PshBI ATTAAT 1 cut(s) 851
PsiI TTATAA 1 cut(s) 78
Psp5II RGGWCCY 1 cut(s) 353
Psp6I CCWGG 2 cut(s) 1179, 1447
PspGI CCWGG 2 cut(s) 1179, 1447
PspN4I GGNNCC 2 cut(s) 354, 441
PspPI GGNCC 4 cut(s) 353, 1089, 1302, 1451
PspPPI RGGWCCY 1 cut(s) 353
PstI CTGCAG 1 cut(s) 1081
PsuI RGATCY 1 cut(s) 827
RsaI GTAC 3 cut(s) 47, 422, 674
RsaNI GTAC 3 cut(s) 46, 421, 673
RseI CAYNNNNRTG 1 cut(s) 1149
SaqAI TTAA 6 cut(s) 120, 417, 456, 530, 648, 851
SatI GCNGC 3 cut(s) 255, 1416, 1512
Sau3AI GATC 7 cut(s) 334, 565, 587, 827, 920, 1138, 1174
Sau96I GGNCC 4 cut(s) 353, 1089, 1302, 1451
ScaI AGTACT 1 cut(s) 47
SchI GAGTC 3 cut(s) 114, 293, 647
ScrFI CCNGG 3 cut(s) 1166, 1181, 1449
SduI GDGCHC 1 cut(s) 238
SfaNI GCATC 1 cut(s) 1200
SfcI CTRYAG 1 cut(s) 1077
SinI GGWCC 2 cut(s) 353, 1089
SmiMI CAYNNNNRTG 1 cut(s) 1149
SmlI CTYRAG 1 cut(s) 738
SmoI CTYRAG 1 cut(s) 738
SnaBI TACGTA 1 cut(s) 672
Sse9I AATT 6 cut(s) 811, 935, 965, 1156, 1380, 1388
SsiI CCGC 4 cut(s) 5, 1288, 1294, 1416
SspMI CTAG 1 cut(s) 1202
StyD4I CCNGG 3 cut(s) 1164, 1179, 1447
StyI CCWWGG 2 cut(s) 618, 1436
TaaI ACNGT 8 cut(s) 100, 175, 425, 820, 1005, 1032, 1148, 1470
TaiI ACGT 2 cut(s) 28, 674
TaqI TCGA 6 cut(s) 384, 572, 1036, 1110, 1137, 1237
TasI AATT 6 cut(s) 811, 935, 965, 1156, 1380, 1388
TatI WGTACW 2 cut(s) 45, 420
TauI GCSGC 1 cut(s) 1418
TfiI GAWTC 5 cut(s) 139, 200, 694, 709, 782
Tru1I TTAA 6 cut(s) 120, 417, 456, 530, 648, 851
Tru9I TTAA 6 cut(s) 120, 417, 456, 530, 648, 851
TscAI CASTG 4 cut(s) 178, 430, 1153, 1479
TseFI GTSAC 3 cut(s) 296, 1375, 1470
TseI GCWGC 2 cut(s) 254, 1511
Tsp45I GTSAC 3 cut(s) 296, 1375, 1470
TspDTI ATGAA 3 cut(s) 461, 488, 509
TspGWI ACGGA 2 cut(s) 36, 558
TspRI CASTG 4 cut(s) 178, 430, 1153, 1479
Van91I CCANNNNNTGG 1 cut(s) 177
VpaK11BI GGWCC 2 cut(s) 353, 1089
VspI ATTAAT 1 cut(s) 851
XapI RAATTY 1 cut(s) 1388
XceI RCATGY 2 cut(s) 371, 1363
XcmI CCANNNNNNNNNTGG 1 cut(s) 850
XmiI GTMKAC 1 cut(s) 557
XspI CTAG 1 cut(s) 1202
ZrmI AGTACT 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.