Rh5AG367800

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0015853
Physical Location & Seq
Reverse (-)
62111749 .. 62115640
3892 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG367800.1

Sequence Viewer

Length: 1389 bp
ATGTACACCGAAGCAGCAATGGATGAGATTAAGATTCTCAATCAGATTGCCGAGGGAGACCCCGGTGACAACAAGTTTGTTGTGAAGCTTTTGGATCATTTTAAGCATTTAGGTCCGAATGGGCAGCATGTCTGTATGGTTTTTGAGTACCTTGGGGATAATCTTTTGACTGTTATTAAGTATAGTGATTACCGAGGGACGCCGTTGCACATGGTTAAAGAGATTGCATTTCATATATTGGTGGGTTTGGATTACTTGCATCGTCAGCTATCGATCATACACACGGATTTGAAGCCAGAGAATGTGTTGCTTTTGTCGATGATTGATCCATCTAAAGATCCGCGGAAGTCAGGTGTTCCTCTTGTCCTTCCAACGGGCAAAGATAAGACTGCGACGGTTTCTGGGGCTTCAAAAGATATTAAGAAGTCAAATGGGGATCTCACCAAGAATCAGAAAAAGAAGATTCGGAAAAAGGCTAAGAAGGCAACTCAGGGTTGTGGAGGGACTGAAACCTCTGAGGATGCTGAGGCAGACTCCACTATAGTCAGTCCAGATGATTCTATTAATGAAGTGAAATCAAATGGGAAAGCTGTGGAAGTACAAGTGAATAATTCTGTGGTTAAAGATGAATCAACTACTGGTGAAGAAGTAAAAGACAATCACCAAGAAAATAAGGATCAGAAAAGACCAGACCCTTCAACAAGGAAGAAGCTGATGGCAGCTGCTGACCTCAAATGCAAATTGGTTGATTTTGGAAGTGCTTGTTGGACATATAAGCAATTCACAAGTGATATTCAGACAAGGCAGTACAGATGCCCAGAAGTTATTCTTGGAGATAAATACTCAACTCCAGCAGATATGTGGTCATTTGCTTGTGTTTGCTTTGAGCTAGCCACTGGTGATGTTCTTTTTGATCCACACAGCGGTGACAAATATGACAAAGATGAGGACCACCTGGCATTGATGATGGAACTTCTTGGAAAAATTCCAAAAAAGATTGCATTAGGTGGTCGCCATTCCCGAGACTTCTTTAACCGTTCTGGGGATTTGAAACATATCCGGCAATTGCGTTTCTGGCCTCTTAATAAGGTGCTAATGGAGAAGTACGGTTTCAGCGAGCAAGATGCAAATGACATGACTAACTTTCTAGTGCCCATACTTGATTATGTCCCTGAAAAGAGGCCAACAGCTGCCCAGTGCCTCAGCCATCCATGGATCAATGCAGGTCCTCAGCTTCTTGAGCCTTCCATGGCTGGCTGGAAATGGGAGTATTTCGGAGAAAAGAGGGAGAAAGATGAGGTGGAAGCATTGGTGGTTGGAATGGAGGATATAGCCATTGATGACTCTTCAAAACCTGTTAAAGAATCCCAACGTGTACCAAATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

462

Amino Acids

51.91

Weight (kDa)

6.05

Isoelectric Point (pI)

30.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 105 1e-12 Protein kinase domain
Pkinase PF00069 242 - 406 5.9e-21 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015853)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44850 AT5G22840
fragaria_vesca FvH4_3g30170
malus_domestica MD03G1130900.v1.1 MD11G1153400.v1.1
prunus_persica Prupe.6G116000_v2.0.a1
pyrus_communis pycom11g12520
rosa_chinensis RchiOBHm_Chr5g0055961
rosa_laevigata RLG00000035037
rosa_multiflora Rmu_sc0000604.1_g000003 Rmu_sc0014108.1_g000001
rosa_roxburghii Rroxscaffold_1G00024340
rosa_rugosa Rorug05G0296400
rosa_samantha Rh5AG367800
rosa_wichuraiana Rw5G034500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1214
AccII CGCG 1 cut(s) 343
AciI CCGC 3 cut(s) 341, 343, 924
AclWI GGATC 7 cut(s) 102, 320, 332, 444, 684, 908, 1223
AcsI RAATTY 2 cut(s) 984, 1381
AcyI GRCGYC 1 cut(s) 200
AfaI GTAC 6 cut(s) 5, 149, 600, 809, 1106, 1377
AfiI CCNNNNNNNGG 3 cut(s) 373, 923, 1042
AflIII ACRYGT 1 cut(s) 1372
AgsI TTSAA 5 cut(s) 292, 411, 699, 1051, 1350
AjnI CCWGG 1 cut(s) 954
AjuI GAANNNNNNNTTGG 4 cut(s) 748, 780, 813, 845
AleI CACNNNNGTG 1 cut(s) 924
AluBI AGCT 8 cut(s) 88, 268, 590, 712, 722, 889, 1190, 1234
AluI AGCT 8 cut(s) 88, 268, 590, 712, 722, 889, 1190, 1234
Alw26I GTCTC 2 cut(s) 51, 1017
AlwI GGATC 7 cut(s) 102, 320, 332, 444, 684, 908, 1223
AlwNI CAGNNNCTG 1 cut(s) 725
Ama87I CYCGRG 1 cut(s) 1020
AoxI GGCC 2 cut(s) 1076, 1181
ApeKI GCWGC 5 cut(s) 14, 124, 719, 722, 1190
ApoI RAATTY 2 cut(s) 984, 1381
ArsI GACNNNNNNTTYG 2 cut(s) 59, 91
AseI ATTAAT 1 cut(s) 564
Asp700I GAANNNNTTC 1 cut(s) 825
AspS9I GGNCC 3 cut(s) 113, 949, 1226
AsuC2I CCSGG 1 cut(s) 63
AsuHPI GGTGA 6 cut(s) 77, 433, 653, 653, 911, 938
AsuNHI GCTAGC 1 cut(s) 889
AvaI CYCGRG 1 cut(s) 1020
AvaII GGWCC 3 cut(s) 113, 949, 1226
BaeGI GKGCMC 1 cut(s) 1155
BbvCI CCTCAGC 3 cut(s) 525, 1202, 1230
BbvI GCAGC 5 cut(s) 26, 136, 709, 731, 1177
BccI CCATC 4 cut(s) 337, 709, 961, 1215
BceAI ACGGC 1 cut(s) 187
BcgI CGANNNNNNTGC 2 cut(s) 1106, 1140
BciT130I CCWGG 1 cut(s) 956
BcnI CCSGG 1 cut(s) 63
BcoDI GTCTC 2 cut(s) 51, 1017
BfaI CTAG 2 cut(s) 890, 1148
BfmI CTRYAG 1 cut(s) 540
BfuAI ACCTGC 1 cut(s) 1214
BisI GCNGC 5 cut(s) 15, 125, 720, 723, 1191
BlsI GCNGC 5 cut(s) 16, 126, 721, 724, 1192
Bme1390I CCNGG 2 cut(s) 63, 956
Bme18I GGWCC 3 cut(s) 113, 949, 1226
BmeT110I CYCGRG 1 cut(s) 1020
BmgT120I GGNCC 3 cut(s) 113, 949, 1226
BmrFI CCNGG 2 cut(s) 63, 956
BmrI ACTGGG 1 cut(s) 1189
BmsI GCATC 4 cut(s) 268, 511, 803, 1114
BmtI GCTAGC 1 cut(s) 893
BmuI ACTGGG 1 cut(s) 1189
BplI GAGNNNNNCTC 2 cut(s) 518, 550
BpmI CTGGAG 1 cut(s) 834
Bpu10I CCTNAGC 3 cut(s) 525, 1202, 1230
BpuEI CTTGAG 1 cut(s) 1259
BpuMI CCSGG 1 cut(s) 63
Bsa29I ATCGAT 1 cut(s) 272
BsaHI GRCGYC 1 cut(s) 200
BsaI GGTCTC 1 cut(s) 51
BsaJI CCNNGG 7 cut(s) 51, 61, 151, 193, 341, 1211, 1248
Bsc4I CCNNNNNNNGG 3 cut(s) 373, 923, 1042
Bse1I ACTGG 3 cut(s) 643, 901, 1195
Bse3DI GCAATG 1 cut(s) 24
BseBI CCWGG 1 cut(s) 956
BseCI ATCGAT 1 cut(s) 272
BseDI CCNNGG 7 cut(s) 51, 61, 151, 193, 341, 1211, 1248
BseGI GGATG 3 cut(s) 28, 526, 1207
BseLI CCNNNNNNNGG 3 cut(s) 373, 923, 1042
BseMI GCAATG 1 cut(s) 24
BseMII CTCAG 5 cut(s) 503, 507, 516, 1216, 1244
BseNI ACTGG 3 cut(s) 643, 901, 1195
BseSI GKGCMC 1 cut(s) 1155
BseXI GCAGC 5 cut(s) 26, 136, 709, 731, 1177
Bsh1236I CGCG 1 cut(s) 343
BshFI GGCC 2 cut(s) 1078, 1183
BshVI ATCGAT 1 cut(s) 272
BsiHKCI CYCGRG 1 cut(s) 1020
BsiSI CCGG 2 cut(s) 63, 1060
BslFI GGGAC 3 cut(s) 211, 517, 1154
BslI CCNNNNNNNGG 3 cut(s) 373, 923, 1042
BsmAI GTCTC 2 cut(s) 51, 1017
BsmFI GGGAC 3 cut(s) 211, 517, 1154
BsnI GGCC 2 cut(s) 1078, 1183
Bso31I GGTCTC 1 cut(s) 51
BsoBI CYCGRG 1 cut(s) 1020
Bsp1286I GDGCHC 1 cut(s) 1155
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 8 cut(s) 94, 273, 325, 337, 436, 676, 913, 1215
Bsp19I CCATGG 2 cut(s) 1211, 1248
BspACI CCGC 3 cut(s) 341, 343, 924
BspANI GGCC 2 cut(s) 1078, 1183
BspCNI CTCAG 5 cut(s) 502, 508, 517, 1215, 1243
BspDI ATCGAT 1 cut(s) 272
BspFNI CGCG 1 cut(s) 343
BspMI ACCTGC 1 cut(s) 1214
BspOI GCTAGC 1 cut(s) 893
BspPI GGATC 7 cut(s) 102, 320, 332, 444, 684, 908, 1223
BspTNI GGTCTC 1 cut(s) 51
BsrDI GCAATG 1 cut(s) 24
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 3 cut(s) 643, 901, 1195
BssECI CCNNGG 7 cut(s) 51, 61, 151, 193, 341, 1211, 1248
BssMI GATC 8 cut(s) 94, 273, 325, 337, 436, 676, 913, 1215
BssNI GRCGYC 1 cut(s) 200
BssT1I CCWWGG 3 cut(s) 151, 1211, 1248
Bst2UI CCWGG 1 cut(s) 956
Bst4CI ACNGT 4 cut(s) 172, 397, 1037, 1109
Bst6I CTCTTC 1 cut(s) 1351
BstACI GRCGYC 1 cut(s) 200
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 3 cut(s) 891, 1118, 1255
BstDEI CTNAG 6 cut(s) 477, 489, 516, 525, 1202, 1230
BstDSI CCRYGG 3 cut(s) 341, 1211, 1248
BstF5I GGATG 3 cut(s) 28, 526, 1207
BstFNI CGCG 1 cut(s) 343
BstKTI GATC 8 cut(s) 97, 276, 328, 340, 439, 679, 916, 1218
BstMAI GTCTC 2 cut(s) 51, 1017
BstMBI GATC 8 cut(s) 94, 273, 325, 337, 436, 676, 913, 1215
BstMWI GCNNNNNNNGC 4 cut(s) 265, 482, 1075, 1240
BstNI CCWGG 1 cut(s) 956
BstNSI RCATGY 1 cut(s) 131
BstSCI CCNGG 2 cut(s) 61, 954
BstSFI CTRYAG 1 cut(s) 540
BstSLI GKGCMC 1 cut(s) 1155
BstUI CGCG 1 cut(s) 343
BstV1I GCAGC 5 cut(s) 26, 136, 709, 731, 1177
BstX2I RGATCY 2 cut(s) 337, 436
BstYI RGATCY 2 cut(s) 337, 436
Bsu15I ATCGAT 1 cut(s) 272
BsuRI GGCC 2 cut(s) 1078, 1183
BsuTUI ATCGAT 1 cut(s) 272
BtgI CCRYGG 3 cut(s) 341, 1211, 1248
BtsCI GGATG 3 cut(s) 28, 526, 1207
BtsIMutI CAGTG 2 cut(s) 894, 1202
BveI ACCTGC 1 cut(s) 1214
Cac8I GCNNGC 3 cut(s) 891, 1118, 1255
CaiI CAGNNNCTG 1 cut(s) 725
Cfr13I GGNCC 3 cut(s) 113, 949, 1226
Cfr42I CCGCGG 1 cut(s) 344
ClaI ATCGAT 1 cut(s) 272
CseI GACGC 1 cut(s) 208
Csp6I GTAC 6 cut(s) 4, 148, 599, 808, 1105, 1376
CviAII CATG 5 cut(s) 128, 211, 1135, 1212, 1249
CviQI GTAC 6 cut(s) 4, 148, 599, 808, 1105, 1376
DdeI CTNAG 6 cut(s) 477, 489, 516, 525, 1202, 1230
DpnI GATC 8 cut(s) 96, 275, 327, 339, 438, 678, 915, 1217
DpnII GATC 8 cut(s) 94, 273, 325, 337, 436, 676, 913, 1215
Eam1104I CTCTTC 1 cut(s) 1351
EarI CTCTTC 1 cut(s) 1351
Eco130I CCWWGG 3 cut(s) 151, 1211, 1248
Eco31I GGTCTC 1 cut(s) 51
Eco47I GGWCC 3 cut(s) 113, 949, 1226
Eco88I CYCGRG 1 cut(s) 1020
EcoO109I RGGNCCY 1 cut(s) 1226
EcoRII CCWGG 1 cut(s) 954
EcoT14I CCWWGG 3 cut(s) 151, 1211, 1248
ErhI CCWWGG 3 cut(s) 151, 1211, 1248
FaeI CATG 5 cut(s) 131, 214, 1138, 1215, 1252
FalI AAGNNNNNCTT 2 cut(s) 813, 845
FaqI GGGAC 3 cut(s) 211, 517, 1154
FatI CATG 5 cut(s) 127, 210, 1134, 1211, 1248
Fnu4HI GCNGC 5 cut(s) 15, 125, 720, 723, 1191
FokI GGATG 3 cut(s) 35, 533, 1194
Fsp4HI GCNGC 5 cut(s) 15, 125, 720, 723, 1191
FspBI CTAG 2 cut(s) 890, 1148
GluI GCNGC 5 cut(s) 15, 125, 720, 723, 1191
GsuI CTGGAG 1 cut(s) 834
HaeIII GGCC 2 cut(s) 1078, 1183
HapII CCGG 2 cut(s) 63, 1060
HgaI GACGC 1 cut(s) 208
Hin1I GRCGYC 1 cut(s) 200
Hin1II CATG 5 cut(s) 131, 214, 1138, 1215, 1252
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 8 cut(s) 34, 448, 463, 533, 557, 629, 1343, 1364
HpaII CCGG 2 cut(s) 63, 1060
HphI GGTGA 6 cut(s) 77, 433, 653, 653, 911, 938
Hpy166II GTNNAC 2 cut(s) 6, 1376
Hpy188I TCNGA 8 cut(s) 45, 117, 453, 468, 517, 681, 798, 1277
Hpy188III TCNNGA 4 cut(s) 551, 1020, 1238, 1386
Hpy8I GTNNAC 2 cut(s) 6, 1376
Hpy99I CGWCG 1 cut(s) 397
HpyAV CCTTC 4 cut(s) 377, 475, 705, 1254
HpyCH4III ACNGT 4 cut(s) 172, 397, 1037, 1109
HpyCH4IV ACGT 1 cut(s) 1372
HpyCH4V TGCA 7 cut(s) 208, 227, 259, 738, 1001, 1127, 1223
HpyF10VI GCNNNNNNNGC 4 cut(s) 265, 482, 1075, 1240
HpyF3I CTNAG 6 cut(s) 477, 489, 516, 525, 1202, 1230
HpySE526I ACGT 1 cut(s) 1372
Hsp92I GRCGYC 1 cut(s) 200
Hsp92II CATG 5 cut(s) 131, 214, 1138, 1215, 1252
KspI CCGCGG 1 cut(s) 344
Kzo9I GATC 8 cut(s) 94, 273, 325, 337, 436, 676, 913, 1215
Lsp1109I GCAGC 5 cut(s) 26, 136, 709, 731, 1177
LweI GCATC 4 cut(s) 268, 511, 803, 1114
MaeI CTAG 2 cut(s) 890, 1148
MaeII ACGT 1 cut(s) 1372
MaeIII GTNAC 2 cut(s) 65, 926
MalI GATC 8 cut(s) 96, 275, 327, 339, 438, 678, 915, 1217
MboI GATC 8 cut(s) 94, 273, 325, 337, 436, 676, 913, 1215
MboII GAAGA 4 cut(s) 472, 656, 718, 1338
MfeI CAATTG 1 cut(s) 1064
MflI RGATCY 2 cut(s) 337, 436
MhlI GDGCHC 1 cut(s) 1155
MluCI AATT 6 cut(s) 610, 740, 779, 984, 1064, 1381
MlyI GAGTC 2 cut(s) 527, 1337
MmeI TCCRAC 3 cut(s) 395, 746, 1297
MroXI GAANNNNTTC 1 cut(s) 825
MslI CAYNNNNRTG 1 cut(s) 924
MspA1I CMGCKG 4 cut(s) 343, 722, 924, 1190
MspI CCGG 2 cut(s) 63, 1060
MspR9I CCNGG 2 cut(s) 63, 956
MunI CAATTG 1 cut(s) 1064
MvaI CCWGG 1 cut(s) 956
MvnI CGCG 1 cut(s) 343
MwoI GCNNNNNNNGC 4 cut(s) 265, 482, 1075, 1240
NciI CCSGG 1 cut(s) 63
NcoI CCATGG 2 cut(s) 1211, 1248
NdeII GATC 8 cut(s) 94, 273, 325, 337, 436, 676, 913, 1215
NheI GCTAGC 1 cut(s) 889
NlaIII CATG 5 cut(s) 131, 214, 1138, 1215, 1252
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 2 cut(s) 65, 926
NspI RCATGY 1 cut(s) 131
OliI CACNNNNGTG 1 cut(s) 924
PcsI WCGNNNNNNNCGW 2 cut(s) 1018, 1113
PdmI GAANNNNTTC 1 cut(s) 825
PfeI GAWTC 6 cut(s) 34, 448, 463, 557, 629, 1364
PkrI GCNGC 5 cut(s) 16, 126, 721, 724, 1192
PleI GAGTC 2 cut(s) 527, 1337
PpsI GAGTC 2 cut(s) 527, 1337
PpuMI RGGWCCY 1 cut(s) 1226
PshBI ATTAAT 1 cut(s) 564
Psp5II RGGWCCY 1 cut(s) 1226
Psp6I CCWGG 1 cut(s) 954
PspGI CCWGG 1 cut(s) 954
PspPI GGNCC 3 cut(s) 113, 949, 1226
PspPPI RGGWCCY 1 cut(s) 1226
PstNI CAGNNNCTG 1 cut(s) 725
PsuI RGATCY 2 cut(s) 337, 436
PvuII CAGCTG 2 cut(s) 722, 1190
RsaI GTAC 6 cut(s) 5, 149, 600, 809, 1106, 1377
RsaNI GTAC 6 cut(s) 4, 148, 599, 808, 1105, 1376
RseI CAYNNNNRTG 1 cut(s) 924
SacII CCGCGG 1 cut(s) 344
SatI GCNGC 5 cut(s) 15, 125, 720, 723, 1191
Sau3AI GATC 8 cut(s) 94, 273, 325, 337, 436, 676, 913, 1215
Sau96I GGNCC 3 cut(s) 113, 949, 1226
SchI GAGTC 2 cut(s) 527, 1337
ScrFI CCNGG 2 cut(s) 63, 956
SduI GDGCHC 1 cut(s) 1155
SfaNI GCATC 4 cut(s) 268, 511, 803, 1114
SfcI CTRYAG 1 cut(s) 540
Sfr303I CCGCGG 1 cut(s) 344
SgrBI CCGCGG 1 cut(s) 344
SinI GGWCC 3 cut(s) 113, 949, 1226
SmiMI CAYNNNNRTG 1 cut(s) 924
SmlI CTYRAG 1 cut(s) 1238
SmoI CTYRAG 1 cut(s) 1238
Sse9I AATT 6 cut(s) 610, 740, 779, 984, 1064, 1381
SsiI CCGC 3 cut(s) 341, 343, 924
SspMI CTAG 2 cut(s) 890, 1148
StyD4I CCNGG 2 cut(s) 61, 954
StyI CCWWGG 3 cut(s) 151, 1211, 1248
TaaI ACNGT 4 cut(s) 172, 397, 1037, 1109
TaiI ACGT 1 cut(s) 1375
TaqI TCGA 2 cut(s) 272, 317
TasI AATT 6 cut(s) 610, 740, 779, 984, 1064, 1381
TatI WGTACW 3 cut(s) 3, 598, 807
TfiI GAWTC 6 cut(s) 34, 448, 463, 557, 629, 1364
TscAI CASTG 2 cut(s) 901, 1202
TseFI GTSAC 2 cut(s) 65, 926
TseI GCWGC 5 cut(s) 14, 124, 719, 722, 1190
Tsp45I GTSAC 2 cut(s) 65, 926
TspDTI ATGAA 3 cut(s) 221, 582, 642
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 2 cut(s) 901, 1202
VpaK11BI GGWCC 3 cut(s) 113, 949, 1226
VspI ATTAAT 1 cut(s) 564
XapI RAATTY 2 cut(s) 984, 1381
XceI RCATGY 1 cut(s) 131
XcmI CCANNNNNNNNNTGG 1 cut(s) 858
XmnI GAANNNNTTC 1 cut(s) 825
XspI CTAG 2 cut(s) 890, 1148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.