Prupe.6G116000_v2.0.a1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0015853
Physical Location & Seq
Forward (+)
8453041 .. 8456415
3375 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G116000.1

Sequence Viewer

Length: 1653 bp
ATGGCGGATGCCCGGGATCGGGACGAGCTCAGCGATTACTCATCGGAGGACGAGGGCACTGAGGACTACAAGAAAGGAGGGTACCATGCGGTCTCTGTTGGGGACTCGTTCAAGAATGGCTGCTATGTGGTGCAGAGTAAGCTGGGCTGGGGTCACTTCTCCACTGTCTGGCTTGCTTGGGACACTCGGAACTCGCGTTATGTAGCTTTGAAGATTCAAAAGAGTGCTCGTTATTACACTGAAGCTGCAATGGACGAAATAAAGATTCTCAAACAAATTGCCGAGGGAGACCCTGGTGACAAGAAGTGTGTTGTGAAGCTTTTGGATCACTTCAAGCATTCGGGCCCAAATGGGCAGCATGTCTGTATGGTTTTTGAGTACCTTGGGGATAACCTTTTGACCCTTATTAAGTATAATGATTACCGAGGAGCTCCAGTGCACATGGTTAAAGAAATTTGTTTCCATATGTTAGTGGGTTTGGATTACTTGCACCGTCAACTTTCAATGATACACACTGATTTGAAGCCAGAGAATGTCTTGCTTTTGTCGATGATCGATCCATCTAAAGATCCCAGGAAATCAGGTGCTCCTCTCATCCTTCCAACCGGTAAGGATAAAACTGCGTCTGTTTCTGGCGCTGCGAAAGACATTAAGAAGTTAAACGGAGATCTCACAAAGAACCAGAAAAAGAAGATCCGGAAAAAGGCTAAGAAGGCAGCTCATGGTTGTGCAGGGATGGAAACTTTGGAGGACAATGAGGCAGATTCCACGATAGTTGGCTCAGAAGATTCCATTAATGAAGTGAAATCAAATGGCGATTCTCTTGAAGAACGAGTGAAGGATTCTGTAGTTGGAAACGAATCAATGAAGGGTGAAGAAACAGAAGAAACTCAGCAAGGAAATACGGAACAAGAAAAAGCCAGCCGTGCTGCAAGGCAGAAGCTGTTCGCAGCTGTTGACCTTAAATGCAAATTGGTTGATTTTGGAAATGCATGTTGGACATATAAGCAGTTTACAAGTGATATTCAAACAAGGCAGTACAGATGCCCAGAAGTTATACTTGGAGCTAAATACTCCACTCCAGCAGATTTATGGTCCTTTGCTTGTGTTTGCTTTGAGCTAGCCACGGGGGACGTTCTTTTCGATCCACACAGTGGTGACAATTATGATAGGGATGAGGATCACCTGGCACTGATGATGGAACTTCTTGGAACAATTCCCCGCAAGACTGCATTAGGTGGTCGCTATTCGCGGGACTTCTTTAACCGATATGGGGATTTGAAGCATATCCGTCGATTGCGTTTTTGGCCTCTAAATAAAGTGCTAATGGAGAAGTACTCATTCAGCGAGCAAGATGCAAATGACATGACTGAGTTCCTTGTTCCCATACTTGATTTTGTCCCTGAAAAGAGGCCAACAGCTGCGCAATGCCTTACTCATCCATGGATCAGTGCAGGCCCTCGACTCCTTGAGCCTTCCGTGGCTGGTATGGAACCTCACGCCTCCAATGGGAGTATTTCTGAGAAAAGGGAGAAGGATGAGAGGGAAGCAATTGAGGTCGGAATGGGTAATATGGCCATTAATGAATCTTCCAAACCTGTTATAGAATCCAAACGTGCACTAAATTCATCACAGGATTCACAACAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

551

Amino Acids

61.68

Weight (kDa)

5.99

Isoelectric Point (pI)

32.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015853)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44850 AT5G22840
fragaria_vesca FvH4_3g30170
malus_domestica MD03G1130900.v1.1 MD11G1153400.v1.1
prunus_persica Prupe.6G116000_v2.0.a1
pyrus_communis pycom11g12520
rosa_chinensis RchiOBHm_Chr5g0055961
rosa_laevigata RLG00000035037
rosa_multiflora Rmu_sc0000604.1_g000003 Rmu_sc0014108.1_g000001
rosa_roxburghii Rroxscaffold_1G00024340
rosa_rugosa Rorug05G0296400
rosa_samantha Rh5AG367800
rosa_wichuraiana Rw5G034500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1425
Acc65I GGTACC 1 cut(s) 81
AccB1I GGYRCC 1 cut(s) 81
AccB7I CCANNNNNTGG 2 cut(s) 168, 1154
AccII CGCG 2 cut(s) 196, 1252
AccIII TCCGGA 1 cut(s) 696
AciI CCGC 4 cut(s) 5, 89, 1222, 1252
AclWI GGATC 8 cut(s) 24, 333, 551, 563, 688, 1139, 1188, 1454
AcoI YGGCCR 1 cut(s) 1575
AcsI RAATTY 2 cut(s) 453, 1624
AcuI CTGAAG 1 cut(s) 261
AdeI CACNNNGTG 1 cut(s) 1154
AfaI GTAC 4 cut(s) 83, 380, 1040, 1337
AfiI CCNNNNNNNGG 7 cut(s) 18, 19, 168, 703, 1154, 1273, 1509
AgeI ACCGGT 1 cut(s) 605
AgsI TTSAA 9 cut(s) 112, 211, 218, 334, 504, 523, 827, 1028, 1282
AjnI CCWGG 3 cut(s) 292, 572, 1185
AjuI GAANNNNNNNTTGG 4 cut(s) 979, 1011, 1044, 1076
AleI CACNNNNGTG 1 cut(s) 1155
Alw21I GWGCWC 6 cut(s) 30, 229, 433, 441, 589, 1621
Alw26I GTCTC 2 cut(s) 97, 282
Alw44I GTGCAC 2 cut(s) 437, 1617
AlwI GGATC 8 cut(s) 24, 333, 551, 563, 688, 1139, 1188, 1454
AlwNI CAGNNNCTG 1 cut(s) 943
Ama87I CYCGRG 1 cut(s) 12
Aor13HI TCCGGA 1 cut(s) 696
AoxI GGCC 5 cut(s) 343, 1307, 1412, 1456, 1575
ApaI GGGCCC 1 cut(s) 347
ApaLI GTGCAC 2 cut(s) 437, 1617
ApeKI GCWGC 8 cut(s) 120, 245, 355, 638, 716, 929, 950, 1421
ApoI RAATTY 2 cut(s) 453, 1624
ArsI GACNNNNNNTTYG 2 cut(s) 1124, 1156
AseI ATTAAT 2 cut(s) 795, 1581
AsiGI ACCGGT 1 cut(s) 605
Asp700I GAANNNNTTC 1 cut(s) 944
Asp718I GGTACC 1 cut(s) 81
AspLEI GCGC 2 cut(s) 638, 1426
AspS9I GGNCC 4 cut(s) 343, 344, 1095, 1457
AsuC2I CCSGG 2 cut(s) 13, 14
AsuHPI GGTGA 4 cut(s) 308, 884, 1169, 1175
AsuNHI GCTAGC 1 cut(s) 1120
AvaI CYCGRG 1 cut(s) 12
AvaII GGWCC 1 cut(s) 1095
BaeGI GKGCMC 4 cut(s) 59, 347, 441, 1621
BalI TGGCCA 1 cut(s) 1577
BanI GGYRCC 1 cut(s) 81
BanII GRGCYC 3 cut(s) 30, 347, 433
Bbv12I GWGCWC 6 cut(s) 30, 229, 433, 441, 589, 1621
BbvI GCAGC 8 cut(s) 107, 232, 367, 625, 728, 916, 962, 1408
BccI CCATC 3 cut(s) 568, 730, 1192
BceAI ACGGC 1 cut(s) 909
BcgI CGANNNNNNTGC 4 cut(s) 1274, 1308, 1337, 1371
BciT130I CCWGG 3 cut(s) 294, 574, 1187
BcnI CCSGG 2 cut(s) 13, 14
BcoDI GTCTC 2 cut(s) 97, 282
BfaI CTAG 1 cut(s) 1121
BfmI CTRYAG 1 cut(s) 846
BfoI RGCGCY 1 cut(s) 639
BglII AGATCT 1 cut(s) 667
BisI GCNGC 8 cut(s) 121, 246, 356, 639, 717, 930, 951, 1422
BlpI GCTNAGC 1 cut(s) 29
BlsI GCNGC 8 cut(s) 122, 247, 357, 640, 718, 931, 952, 1423
BmcAI AGTACT 1 cut(s) 1337
Bme1390I CCNGG 5 cut(s) 13, 14, 294, 574, 1187
Bme18I GGWCC 1 cut(s) 1095
BmeT110I CYCGRG 1 cut(s) 12
BmgT120I GGNCC 4 cut(s) 343, 344, 1095, 1457
BmiI GGNNCC 3 cut(s) 83, 345, 1494
BmrFI CCNGG 5 cut(s) 13, 14, 294, 574, 1187
BmsI GCATC 2 cut(s) 1034, 1345
BmtI GCTAGC 1 cut(s) 1124
BplI GAGNNNNNCTC 2 cut(s) 1322, 1354
BpmI CTGGAG 2 cut(s) 417, 1065
Bpu1102I GCTNAGC 1 cut(s) 29
BpuEI CTTGAG 1 cut(s) 1490
BpuMI CCSGG 2 cut(s) 13, 14
Bsa29I ATCGAT 1 cut(s) 555
BsaBI GATNNNNATC 1 cut(s) 1179
BsaI GGTCTC 2 cut(s) 97, 282
BsaJI CCNNGG 9 cut(s) 12, 282, 292, 382, 424, 572, 1125, 1442, 1479
BsaWI WCCGGW 2 cut(s) 605, 696
BsaXI ACNNNNNCTCC 2 cut(s) 420, 450
Bsc4I CCNNNNNNNGG 7 cut(s) 18, 19, 168, 703, 1154, 1273, 1509
Bse118I RCCGGY 1 cut(s) 605
Bse1I ACTGG 1 cut(s) 434
Bse3DI GCAATG 2 cut(s) 255, 1433
Bse8I GATNNNNATC 1 cut(s) 1179
BseAI TCCGGA 1 cut(s) 696
BseBI CCWGG 3 cut(s) 294, 574, 1187
BseCI ATCGAT 1 cut(s) 555
BseDI CCNNGG 9 cut(s) 12, 282, 292, 382, 424, 572, 1125, 1442, 1479
BseGI GGATG 6 cut(s) 13, 594, 741, 1180, 1438, 1543
BseJI GATNNNNATC 1 cut(s) 1179
BseLI CCNNNNNNNGG 7 cut(s) 18, 19, 168, 703, 1154, 1273, 1509
BseMI GCAATG 2 cut(s) 255, 1433
BseMII CTCAG 6 cut(s) 43, 51, 795, 905, 1362, 1512
BseNI ACTGG 1 cut(s) 434
BseRI GAGGAG 2 cut(s) 441, 579
BseSI GKGCMC 4 cut(s) 59, 347, 441, 1621
BseXI GCAGC 8 cut(s) 107, 232, 367, 625, 728, 916, 962, 1408
BseYI CCCAGC 2 cut(s) 142, 147
BsgI GTGCAG 3 cut(s) 152, 750, 1473
Bsh1236I CGCG 2 cut(s) 196, 1252
BshFI GGCC 5 cut(s) 345, 1309, 1414, 1458, 1577
BshNI GGYRCC 1 cut(s) 81
BshTI ACCGGT 1 cut(s) 605
BshVI ATCGAT 1 cut(s) 555
BsiHKAI GWGCWC 6 cut(s) 30, 229, 433, 441, 589, 1621
BsiHKCI CYCGRG 1 cut(s) 12
BsiSI CCGG 3 cut(s) 13, 606, 697
BslFI GGGAC 6 cut(s) 35, 116, 194, 1145, 1268, 1385
BslI CCNNNNNNNGG 7 cut(s) 18, 19, 168, 703, 1154, 1273, 1509
BsmAI GTCTC 2 cut(s) 97, 282
BsmFI GGGAC 6 cut(s) 35, 116, 194, 1145, 1268, 1385
BsmI GAATGC 1 cut(s) 337
BsnI GGCC 5 cut(s) 345, 1309, 1414, 1458, 1577
Bso31I GGTCTC 2 cut(s) 97, 282
BsoBI CYCGRG 1 cut(s) 12
Bsp120I GGGCCC 1 cut(s) 343
Bsp1286I GDGCHC 8 cut(s) 30, 59, 229, 347, 433, 441, 589, 1621
Bsp13I TCCGGA 1 cut(s) 696
Bsp1720I GCTNAGC 1 cut(s) 29
Bsp19I CCATGG 1 cut(s) 1442
BspACI CCGC 4 cut(s) 5, 89, 1222, 1252
BspANI GGCC 5 cut(s) 345, 1309, 1414, 1458, 1577
BspCNI CTCAG 6 cut(s) 42, 52, 794, 904, 1363, 1513
BspDI ATCGAT 1 cut(s) 555
BspEI TCCGGA 1 cut(s) 696
BspFNI CGCG 2 cut(s) 196, 1252
BspLI GGNNCC 3 cut(s) 83, 345, 1494
BspOI GCTAGC 1 cut(s) 1124
BspPI GGATC 8 cut(s) 24, 333, 551, 563, 688, 1139, 1188, 1454
BspT107I GGYRCC 1 cut(s) 81
BspTNI GGTCTC 2 cut(s) 97, 282
BsrDI GCAATG 2 cut(s) 255, 1433
BsrFI RCCGGY 1 cut(s) 605
BsrI ACTGG 1 cut(s) 434
BssAI RCCGGY 1 cut(s) 605
BssECI CCNNGG 9 cut(s) 12, 282, 292, 382, 424, 572, 1125, 1442, 1479
BssT1I CCWWGG 2 cut(s) 382, 1442
Bst2UI CCWGG 3 cut(s) 294, 574, 1187
Bst4CI ACNGT 3 cut(s) 166, 494, 1154
BstC8I GCNNGC 5 cut(s) 174, 922, 1122, 1349, 1456
BstDEI CTNAG 7 cut(s) 29, 60, 708, 781, 891, 1371, 1521
BstDSI CCRYGG 3 cut(s) 1125, 1442, 1479
BstF5I GGATG 6 cut(s) 13, 594, 741, 1180, 1438, 1543
BstFNI CGCG 2 cut(s) 196, 1252
BstH2I RGCGCY 1 cut(s) 639
BstHHI GCGC 2 cut(s) 638, 1426
BstMAI GTCTC 2 cut(s) 97, 282
BstMWI GCNNNNNNNGC 4 cut(s) 139, 713, 926, 1306
BstNI CCWGG 3 cut(s) 294, 574, 1187
BstNSI RCATGY 2 cut(s) 362, 996
BstSCI CCNGG 5 cut(s) 11, 12, 292, 572, 1185
BstSFI CTRYAG 1 cut(s) 846
BstSLI GKGCMC 4 cut(s) 59, 347, 441, 1621
BstUI CGCG 2 cut(s) 196, 1252
BstV1I GCAGC 8 cut(s) 107, 232, 367, 625, 728, 916, 962, 1408
BstX2I RGATCY 3 cut(s) 568, 667, 693
BstYI RGATCY 3 cut(s) 568, 667, 693
Bsu15I ATCGAT 1 cut(s) 555
BsuRI GGCC 5 cut(s) 345, 1309, 1414, 1458, 1577
BsuTUI ATCGAT 1 cut(s) 555
BtgI CCRYGG 3 cut(s) 1125, 1442, 1479
BtsCI GGATG 6 cut(s) 13, 594, 741, 1180, 1438, 1543
BtsIMutI CAGTG 8 cut(s) 57, 162, 237, 441, 513, 1159, 1190, 1456
Cac8I GCNNGC 5 cut(s) 174, 922, 1122, 1349, 1456
CaiI CAGNNNCTG 1 cut(s) 943
CfoI GCGC 2 cut(s) 638, 1426
Cfr10I RCCGGY 1 cut(s) 605
Cfr13I GGNCC 4 cut(s) 343, 344, 1095, 1457
Cfr9I CCCGGG 1 cut(s) 12
ClaI ATCGAT 1 cut(s) 555
CseI GACGC 1 cut(s) 612
Csp6I GTAC 4 cut(s) 82, 379, 1039, 1336
CspAI ACCGGT 1 cut(s) 605
CspCI CAANNNNNGTGG 2 cut(s) 757, 792
CviAII CATG 8 cut(s) 86, 359, 442, 722, 993, 1366, 1443, 1650
CviQI GTAC 4 cut(s) 82, 379, 1039, 1336
DdeI CTNAG 7 cut(s) 29, 60, 708, 781, 891, 1371, 1521
DraIII CACNNNGTG 1 cut(s) 1154
EaeI YGGCCR 1 cut(s) 1575
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 2 cut(s) 28, 431
Eco130I CCWWGG 2 cut(s) 382, 1442
Eco24I GRGCYC 3 cut(s) 30, 347, 433
Eco31I GGTCTC 2 cut(s) 97, 282
Eco47I GGWCC 1 cut(s) 1095
Eco53kI GAGCTC 2 cut(s) 28, 431
Eco57I CTGAAG 1 cut(s) 261
Eco88I CYCGRG 1 cut(s) 12
EcoICRI GAGCTC 2 cut(s) 28, 431
EcoO109I RGGNCCY 1 cut(s) 1457
EcoRII CCWGG 3 cut(s) 292, 572, 1185
EcoT14I CCWWGG 2 cut(s) 382, 1442
EcoT22I ATGCAT 1 cut(s) 994
EcoT38I GRGCYC 3 cut(s) 30, 347, 433
ErhI CCWWGG 2 cut(s) 382, 1442
FaeI CATG 8 cut(s) 89, 362, 445, 725, 996, 1369, 1446, 1653
FalI AAGNNNNNCTT 2 cut(s) 1044, 1076
FaqI GGGAC 6 cut(s) 35, 116, 194, 1145, 1268, 1385
FatI CATG 8 cut(s) 85, 358, 441, 721, 992, 1365, 1442, 1649
FauI CCCGC 2 cut(s) 1229, 1245
FauNDI CATATG 1 cut(s) 465
Fnu4HI GCNGC 8 cut(s) 121, 246, 356, 639, 717, 930, 951, 1422
FokI GGATG 6 cut(s) 20, 581, 748, 1187, 1425, 1550
FriOI GRGCYC 3 cut(s) 30, 347, 433
Fsp4HI GCNGC 8 cut(s) 121, 246, 356, 639, 717, 930, 951, 1422
FspBI CTAG 1 cut(s) 1121
FspI TGCGCA 1 cut(s) 1425
GlaI GCGC 2 cut(s) 637, 1425
GluI GCNGC 8 cut(s) 121, 246, 356, 639, 717, 930, 951, 1422
GsaI CCCAGC 2 cut(s) 146, 151
GsuI CTGGAG 2 cut(s) 417, 1065
HaeII RGCGCY 1 cut(s) 639
HaeIII GGCC 5 cut(s) 345, 1309, 1414, 1458, 1577
HapII CCGG 3 cut(s) 13, 606, 697
HgaI GACGC 1 cut(s) 612
HhaI GCGC 2 cut(s) 638, 1426
Hin1II CATG 8 cut(s) 89, 362, 445, 725, 996, 1369, 1446, 1653
Hin6I GCGC 2 cut(s) 636, 1424
HinP1I GCGC 2 cut(s) 636, 1424
HincII GTYRAC 2 cut(s) 497, 958
HindII GTYRAC 2 cut(s) 497, 958
HindIII AAGCTT 1 cut(s) 317
HpaII CCGG 3 cut(s) 13, 606, 697
HphI GGTGA 4 cut(s) 308, 884, 1169, 1175
Hpy166II GTNNAC 5 cut(s) 439, 497, 958, 1014, 1619
Hpy188I TCNGA 5 cut(s) 46, 189, 784, 1522, 1562
Hpy188III TCNNGA 4 cut(s) 20, 112, 697, 824
Hpy8I GTNNAC 5 cut(s) 439, 497, 958, 1014, 1619
Hpy99I CGWCG 1 cut(s) 1296
HpyAV CCTTC 6 cut(s) 608, 706, 832, 862, 1485, 1528
HpyCH4III ACNGT 3 cut(s) 166, 494, 1154
HpyCH4IV ACGT 2 cut(s) 1134, 1615
HpyF10VI GCNNNNNNNGC 4 cut(s) 139, 713, 926, 1306
HpyF3I CTNAG 7 cut(s) 29, 60, 708, 781, 891, 1371, 1521
HpySE526I ACGT 2 cut(s) 1134, 1615
Hsp92II CATG 8 cut(s) 89, 362, 445, 725, 996, 1369, 1446, 1653
HspAI GCGC 2 cut(s) 636, 1424
Kpn2I TCCGGA 1 cut(s) 696
KpnI GGTACC 1 cut(s) 85
LmnI GCTCC 4 cut(s) 428, 436, 592, 1064
Lsp1109I GCAGC 8 cut(s) 107, 232, 367, 625, 728, 916, 962, 1408
LweI GCATC 2 cut(s) 1034, 1345
MaeI CTAG 1 cut(s) 1121
MaeII ACGT 2 cut(s) 1134, 1615
MaeIII GTNAC 3 cut(s) 152, 296, 1157
MboII GAAGA 7 cut(s) 223, 703, 797, 839, 887, 896, 1581
MfeI CAATTG 1 cut(s) 1551
MflI RGATCY 3 cut(s) 568, 667, 693
MhlI GDGCHC 8 cut(s) 30, 59, 229, 347, 433, 441, 589, 1621
MlsI TGGCCA 1 cut(s) 1577
MluCI AATT 7 cut(s) 276, 453, 971, 1162, 1215, 1551, 1624
MluNI TGGCCA 1 cut(s) 1577
MlyI GAGTC 2 cut(s) 98, 1458
MmeI TCCRAC 4 cut(s) 626, 832, 977, 1540
Mox20I TGGCCA 1 cut(s) 1577
Mph1103I ATGCAT 1 cut(s) 994
MroI TCCGGA 1 cut(s) 696
MroXI GAANNNNTTC 1 cut(s) 944
MscI TGGCCA 1 cut(s) 1577
MseI TTAA 8 cut(s) 408, 447, 651, 659, 795, 963, 1263, 1581
MslI CAYNNNNRTG 2 cut(s) 726, 1155
Msp20I TGGCCA 1 cut(s) 1577
MspA1I CMGCKG 2 cut(s) 953, 1421
MspI CCGG 3 cut(s) 13, 606, 697
MspR9I CCNGG 5 cut(s) 13, 14, 294, 574, 1187
MunI CAATTG 1 cut(s) 1551
Mva1269I GAATGC 1 cut(s) 337
MvaI CCWGG 3 cut(s) 294, 574, 1187
MvnI CGCG 2 cut(s) 196, 1252
MwoI GCNNNNNNNGC 4 cut(s) 139, 713, 926, 1306
NciI CCSGG 2 cut(s) 13, 14
NcoI CCATGG 1 cut(s) 1442
NdeI CATATG 1 cut(s) 465
NheI GCTAGC 1 cut(s) 1120
NlaIII CATG 8 cut(s) 89, 362, 445, 725, 996, 1369, 1446, 1653
NlaIV GGNNCC 3 cut(s) 83, 345, 1494
NmeAIII GCCGAG 1 cut(s) 307
NmuCI GTSAC 3 cut(s) 152, 296, 1157
NsbI TGCGCA 1 cut(s) 1425
NsiI ATGCAT 1 cut(s) 994
NspI RCATGY 2 cut(s) 362, 996
OliI CACNNNNGTG 1 cut(s) 1155
PcsI WCGNNNNNNNCGW 3 cut(s) 30, 193, 1140
PctI GAATGC 1 cut(s) 337
PdmI GAANNNNTTC 1 cut(s) 944
PflMI CCANNNNNTGG 2 cut(s) 168, 1154
PinAI ACCGGT 1 cut(s) 605
PkrI GCNGC 8 cut(s) 122, 247, 357, 640, 718, 931, 952, 1423
PleI GAGTC 2 cut(s) 98, 1458
PpsI GAGTC 2 cut(s) 98, 1458
PshBI ATTAAT 2 cut(s) 795, 1581
Psp124BI GAGCTC 2 cut(s) 30, 433
Psp6I CCWGG 3 cut(s) 292, 572, 1185
PspFI CCCAGC 2 cut(s) 142, 147
PspGI CCWGG 3 cut(s) 292, 572, 1185
PspN4I GGNNCC 3 cut(s) 83, 345, 1494
PspOMI GGGCCC 1 cut(s) 343
PspPI GGNCC 4 cut(s) 343, 344, 1095, 1457
PstNI CAGNNNCTG 1 cut(s) 943
PsuI RGATCY 3 cut(s) 568, 667, 693
PvuII CAGCTG 2 cut(s) 953, 1421
RsaI GTAC 4 cut(s) 83, 380, 1040, 1337
RsaNI GTAC 4 cut(s) 82, 379, 1039, 1336
RseI CAYNNNNRTG 2 cut(s) 726, 1155
SacI GAGCTC 2 cut(s) 30, 433
SaqAI TTAA 8 cut(s) 408, 447, 651, 659, 795, 963, 1263, 1581
SatI GCNGC 8 cut(s) 121, 246, 356, 639, 717, 930, 951, 1422
Sau96I GGNCC 4 cut(s) 343, 344, 1095, 1457
ScaI AGTACT 1 cut(s) 1337
SchI GAGTC 2 cut(s) 98, 1458
ScrFI CCNGG 5 cut(s) 13, 14, 294, 574, 1187
SduI GDGCHC 8 cut(s) 30, 59, 229, 347, 433, 441, 589, 1621
SfaNI GCATC 2 cut(s) 1034, 1345
SfcI CTRYAG 1 cut(s) 846
SinI GGWCC 1 cut(s) 1095
SmaI CCCGGG 1 cut(s) 14
SmiMI CAYNNNNRTG 2 cut(s) 726, 1155
SmlI CTYRAG 1 cut(s) 1469
SmoI CTYRAG 1 cut(s) 1469
Sse9I AATT 7 cut(s) 276, 453, 971, 1162, 1215, 1551, 1624
SsiI CCGC 4 cut(s) 5, 89, 1222, 1252
SspMI CTAG 1 cut(s) 1121
SstI GAGCTC 2 cut(s) 30, 433
StyD4I CCNGG 5 cut(s) 11, 12, 292, 572, 1185
StyI CCWWGG 2 cut(s) 382, 1442
TaaI ACNGT 3 cut(s) 166, 494, 1154
TaiI ACGT 2 cut(s) 1137, 1618
TaqI TCGA 5 cut(s) 548, 555, 1143, 1294, 1462
TasI AATT 7 cut(s) 276, 453, 971, 1162, 1215, 1551, 1624
TatI WGTACW 2 cut(s) 1038, 1335
Tru1I TTAA 8 cut(s) 408, 447, 651, 659, 795, 963, 1263, 1581
Tru9I TTAA 8 cut(s) 408, 447, 651, 659, 795, 963, 1263, 1581
TscAI CASTG 8 cut(s) 64, 169, 244, 441, 520, 1159, 1197, 1456
TseFI GTSAC 3 cut(s) 152, 296, 1157
TseI GCWGC 8 cut(s) 120, 245, 355, 638, 716, 929, 950, 1421
Tsp45I GTSAC 3 cut(s) 152, 296, 1157
TspDTI ATGAA 4 cut(s) 813, 881, 1599, 1617
TspGWI ACGGA 4 cut(s) 678, 920, 1280, 1468
TspMI CCCGGG 1 cut(s) 12
TspRI CASTG 8 cut(s) 64, 169, 244, 441, 520, 1159, 1197, 1456
Van91I CCANNNNNTGG 2 cut(s) 168, 1154
VneI GTGCAC 2 cut(s) 437, 1617
VpaK11BI GGWCC 1 cut(s) 1095
VspI ATTAAT 2 cut(s) 795, 1581
XapI RAATTY 2 cut(s) 453, 1624
XceI RCATGY 2 cut(s) 362, 996
XcmI CCANNNNNNNNNTGG 1 cut(s) 1089
XmaI CCCGGG 1 cut(s) 12
XmnI GAANNNNTTC 1 cut(s) 944
XspI CTAG 1 cut(s) 1121
ZrmI AGTACT 1 cut(s) 1337
Zsp2I ATGCAT 1 cut(s) 994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.