MD03G1130900.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015853
Physical Location & Seq
Forward (+)
12904833 .. 12908071
3239 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1130900.v1.1.491

Sequence Viewer

Length: 1623 bp
ATGGCGGATGACCGGGACGAGCTCAGCGATTACACATCGGAAGACGAAGGCACCGAGGATTACAAGAAGGGAGGCTACCACGCCGTCTCTATTGGGGACTCCTTCAAGAATGGCTGCTATGTGGTTCAGAGCAAGCTCGGGTGGGGCCACTTCTCCACCGTCTGGCTTGCGTGGGACACAAAGAGCTCGCGTTATGTAGCTTTGAAGATTCAAAAGAGTTCTCGTTATTACACTGAAGCTGCGATGGACGAAATAAAGATTCTCAAACAGATTGCCGAGGGAGACCCTGGCAATAAGAAGTGTGTTGTGAAGCTTTTGGATCACTTCAAGCATTCAGGCCCAAATGGACAGCACGTCTGTTTGGTTTTTGAGTACCTGGGGGATAACCTCCTGACCCTTATTAAGTATAATGACTACCGAGGGGCTCCGCTGCACATGGTCAAAGAAATTTGTTTTCATATATTAGTGGGTTTGGATTATTTGCATAGTCAACTTTCAATAATACACACTGATCTAAAGCCAGAGAATGTTTTGCTTTCGTCAATGATTGATCCATCTAAAGATCCTAGGAAATCAGGTGCTCCTCTCATCCTTCCAACCAGCAAAGATAAAACTGTGCCCGTCTCTGGGGCTTCTAAAGACATTAAGAAGTTAAACGGAGATCTCACCAAAAACCAGAAAAAGAAGATCCGTAAAAAGGCTAAGAAGGCAACTCAAGGTTGTGAAGGAAGGGAAATTTCGAAGGATATGGAGGCAGATTCCACTACAGTTGATGCGGAAGATTCTATTAATGAAGTACAATCAAATGGGGTTTCTGTTCAAGAACAAGTGAAGGATTCTGGGGTTAGTAATGAATCAGTAAAGGGTGAAGAAAAGGAAGAAACTCAAGAAGCACAAGCAAGCCGTTCTGCAAGGCAGAAGTTATTCACAGAAGCTGACCTTAAATGCAAATTGGTTGATTTTGGAAATGCTTGTTGGACGTATAAGCAGTTTACTAGTGATATTCAGACAAGGCAGTACAGATGTCCAGAAGTTATTCTTGGAGCGAAATACTCAACTCCAGCAGATTTATGGTCCTTTGCTTGTGTTTGTTTTGAGCTAGCCACTGGTGACGTTCTTTTTGATCCACACAGCGGTGACAATTATGATCGGGATGAGGATCACCTGGCACTGATGATGGAACTTCTTGGAACAATTCCCCGTAAGACTGCATTAGGTGGCAAGTATTCCCGGGACTTCTTTAACCGGTATGGGGATTTGAAGCATATTCGACGTTTGCGTTTCTGGCCTCTTAATAAGGTGCTAACGGAGAAGTACGCATTCAGCGAGCAAGATGCAAATGACATGGCAGAGTTTCTGGTTCCCATACTTGATTTTGTGCCTGAAAAGAGGCCAACAGCTGCACAATGCCTCAGTCATTCTTGGTTCAGTGCAGGTCCTCGACTCCTTGAGCCTTCCGTGGCTGGTGTGCAACCTCAGGCGTCCAACGGGAGTATTTCTGAGAAGGTGGAGAAGGATGGGAGGGAAGCAATGGAGGTGGGAATGGGGAATATAGCCATTGATGAATCTTCAAAACCTGTTAAAGAATCCCAAAGTGCACTAAATTCATCACAACAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

541

Amino Acids

60.29

Weight (kDa)

5.89

Isoelectric Point (pI)

35.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 40 - 180 1.3e-20 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 42 - 181 7.8e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 312 - 476 5.3e-19 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015853)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44850 AT5G22840
fragaria_vesca FvH4_3g30170
malus_domestica MD03G1130900.v1.1 MD11G1153400.v1.1
prunus_persica Prupe.6G116000_v2.0.a1
pyrus_communis pycom11g12520
rosa_chinensis RchiOBHm_Chr5g0055961
rosa_laevigata RLG00000035037
rosa_multiflora Rmu_sc0000604.1_g000003 Rmu_sc0014108.1_g000001
rosa_roxburghii Rroxscaffold_1G00024340
rosa_rugosa Rorug05G0296400
rosa_samantha Rh5AG367800
rosa_wichuraiana Rw5G034500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1424
AccB1I GGYRCC 1 cut(s) 50
AccB7I CCANNNNNTGG 1 cut(s) 162
AccII CGCG 1 cut(s) 190
AciI CCGC 4 cut(s) 5, 428, 776, 1134
AclWI GGATC 6 cut(s) 327, 545, 557, 682, 1118, 1167
AcsI RAATTY 3 cut(s) 447, 735, 1603
AcuI CTGAAG 1 cut(s) 255
AcyI GRCGYC 1 cut(s) 1481
AfaI GTAC 4 cut(s) 374, 798, 1019, 1316
AfiI CCNNNNNNNGG 6 cut(s) 162, 626, 627, 697, 1133, 1252
AgeI ACCGGT 1 cut(s) 1245
AgsI TTSAA 8 cut(s) 106, 205, 212, 328, 498, 821, 1261, 1572
AhdI GACNNNNNGTC 1 cut(s) 353
AhlI ACTAGT 1 cut(s) 995
AjiI CACGTC 1 cut(s) 355
AjnI CCWGG 3 cut(s) 286, 375, 1164
AjuI GAANNNNNNNTTGG 4 cut(s) 958, 990, 1023, 1055
AleI CACNNNNGTG 1 cut(s) 1134
AluBI AGCT 9 cut(s) 22, 136, 186, 200, 239, 313, 935, 1099, 1400
AluI AGCT 9 cut(s) 22, 136, 186, 200, 239, 313, 935, 1099, 1400
Alw21I GWGCWC 4 cut(s) 24, 188, 583, 1600
Alw26I GTCTC 3 cut(s) 91, 276, 628
Alw44I GTGCAC 1 cut(s) 1596
AlwI GGATC 6 cut(s) 327, 545, 557, 682, 1118, 1167
AlwNI CAGNNNCTG 1 cut(s) 935
Ama87I CYCGRG 2 cut(s) 137, 1230
AoxI GGCC 4 cut(s) 145, 337, 1286, 1391
ApaLI GTGCAC 1 cut(s) 1596
ApeKI GCWGC 4 cut(s) 114, 239, 430, 1400
ApoI RAATTY 3 cut(s) 447, 735, 1603
ArsI GACNNNNNNTTYG 2 cut(s) 1103, 1135
AseI ATTAAT 1 cut(s) 789
AsiGI ACCGGT 1 cut(s) 1245
Asp700I GAANNNNTTC 2 cut(s) 923, 1035
AspA2I CCTAGG 1 cut(s) 566
AspS9I GGNCC 4 cut(s) 145, 338, 1074, 1436
AsuC2I CCSGG 3 cut(s) 14, 1231, 1232
AsuHPI GGTGA 5 cut(s) 658, 878, 1121, 1148, 1154
AsuII TTCGAA 1 cut(s) 740
AsuNHI GCTAGC 1 cut(s) 1099
AvaI CYCGRG 2 cut(s) 137, 1230
AvaII GGWCC 2 cut(s) 1074, 1436
AvrII CCTAGG 1 cut(s) 566
AxyI CCTNAGG 1 cut(s) 1476
BaeGI GKGCMC 2 cut(s) 621, 1600
BanI GGYRCC 1 cut(s) 50
BanII GRGCYC 3 cut(s) 24, 188, 427
BarI GAAGNNNNNNTAC 2 cut(s) 59, 91
BbsI GAAGAC 1 cut(s) 48
Bbv12I GWGCWC 4 cut(s) 24, 188, 583, 1600
BbvI GCAGC 4 cut(s) 101, 226, 417, 1387
BccI CCATC 4 cut(s) 238, 562, 1171, 1511
BceAI ACGGC 2 cut(s) 68, 888
BcgI CGANNNNNNTGC 2 cut(s) 1316, 1350
BciT130I CCWGG 3 cut(s) 288, 377, 1166
BcnI CCSGG 3 cut(s) 14, 1231, 1232
BcoDI GTCTC 3 cut(s) 91, 276, 628
BcuI ACTAGT 1 cut(s) 995
BfaI CTAG 3 cut(s) 567, 996, 1100
BfmI CTRYAG 1 cut(s) 765
BfuAI ACCTGC 1 cut(s) 1424
BglII AGATCT 1 cut(s) 661
BisI GCNGC 4 cut(s) 115, 240, 431, 1401
BlnI CCTAGG 1 cut(s) 566
BlpI GCTNAGC 1 cut(s) 23
BlsI GCNGC 4 cut(s) 116, 241, 432, 1402
Bme1390I CCNGG 6 cut(s) 14, 288, 377, 1166, 1231, 1232
Bme18I GGWCC 2 cut(s) 1074, 1436
BmeRI GACNNNNNGTC 1 cut(s) 353
BmeT110I CYCGRG 2 cut(s) 137, 1230
BmgBI CACGTC 1 cut(s) 355
BmgT120I GGNCC 4 cut(s) 145, 338, 1074, 1436
BmiI GGNNCC 4 cut(s) 52, 146, 426, 1362
BmrFI CCNGG 6 cut(s) 14, 288, 377, 1166, 1231, 1232
BmsI GCATC 2 cut(s) 763, 1324
BmtI GCTAGC 1 cut(s) 1103
BpiI GAAGAC 1 cut(s) 48
BpmI CTGGAG 1 cut(s) 1044
Bpu1102I GCTNAGC 1 cut(s) 23
Bpu14I TTCGAA 1 cut(s) 740
BpuEI CTTGAG 3 cut(s) 699, 870, 1469
BpuMI CCSGG 3 cut(s) 14, 1231, 1232
BsaBI GATNNNNATC 1 cut(s) 1158
BsaHI GRCGYC 1 cut(s) 1481
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 8 cut(s) 54, 276, 286, 376, 418, 566, 1230, 1458
BsaWI WCCGGW 1 cut(s) 1245
Bsc4I CCNNNNNNNGG 6 cut(s) 162, 626, 627, 697, 1133, 1252
Bse118I RCCGGY 1 cut(s) 1245
Bse1I ACTGG 1 cut(s) 1111
Bse21I CCTNAGG 1 cut(s) 1476
Bse3DI GCAATG 1 cut(s) 1536
Bse8I GATNNNNATC 1 cut(s) 1158
BseBI CCWGG 3 cut(s) 288, 377, 1166
BseDI CCNNGG 8 cut(s) 54, 276, 286, 376, 418, 566, 1230, 1458
BseGI GGATG 4 cut(s) 13, 588, 1159, 1522
BseJI GATNNNNATC 1 cut(s) 1158
BseLI CCNNNNNNNGG 6 cut(s) 162, 626, 627, 697, 1133, 1252
BseMI GCAATG 1 cut(s) 1536
BseMII CTCAG 4 cut(s) 37, 1426, 1490, 1491
BseNI ACTGG 1 cut(s) 1111
BseRI GAGGAG 1 cut(s) 573
BseSI GKGCMC 2 cut(s) 621, 1600
BseXI GCAGC 4 cut(s) 101, 226, 417, 1387
BsgI GTGCAG 3 cut(s) 416, 1386, 1452
Bsh1236I CGCG 1 cut(s) 190
BshFI GGCC 4 cut(s) 147, 339, 1288, 1393
BshNI GGYRCC 1 cut(s) 50
BshTI ACCGGT 1 cut(s) 1245
BsiHKAI GWGCWC 4 cut(s) 24, 188, 583, 1600
BsiHKCI CYCGRG 2 cut(s) 137, 1230
BsiSI CCGG 3 cut(s) 13, 1231, 1246
BslFI GGGAC 4 cut(s) 29, 110, 188, 1247
BslI CCNNNNNNNGG 6 cut(s) 162, 626, 627, 697, 1133, 1252
BsmAI GTCTC 3 cut(s) 91, 276, 628
BsmBI CGTCTC 2 cut(s) 91, 628
BsmFI GGGAC 4 cut(s) 29, 110, 188, 1247
BsmI GAATGC 2 cut(s) 331, 1319
BsnI GGCC 4 cut(s) 147, 339, 1288, 1393
Bso31I GGTCTC 1 cut(s) 276
BsoBI CYCGRG 2 cut(s) 137, 1230
Bsp119I TTCGAA 1 cut(s) 740
Bsp1286I GDGCHC 6 cut(s) 24, 188, 427, 583, 621, 1600
Bsp143I GATC 9 cut(s) 319, 511, 550, 562, 661, 687, 1123, 1147, 1159
Bsp1720I GCTNAGC 1 cut(s) 23
BspACI CCGC 4 cut(s) 5, 428, 776, 1134
BspANI GGCC 4 cut(s) 147, 339, 1288, 1393
BspCNI CTCAG 4 cut(s) 36, 1425, 1489, 1492
BspFNI CGCG 1 cut(s) 190
BspLI GGNNCC 4 cut(s) 52, 146, 426, 1362
BspMI ACCTGC 1 cut(s) 1424
BspOI GCTAGC 1 cut(s) 1103
BspPI GGATC 6 cut(s) 327, 545, 557, 682, 1118, 1167
BspT104I TTCGAA 1 cut(s) 740
BspT107I GGYRCC 1 cut(s) 50
BspTNI GGTCTC 1 cut(s) 276
BsrDI GCAATG 1 cut(s) 1536
BsrFI RCCGGY 1 cut(s) 1245
BsrI ACTGG 1 cut(s) 1111
BssAI RCCGGY 1 cut(s) 1245
BssECI CCNNGG 8 cut(s) 54, 276, 286, 376, 418, 566, 1230, 1458
BssMI GATC 9 cut(s) 319, 511, 550, 562, 661, 687, 1123, 1147, 1159
BssNI GRCGYC 1 cut(s) 1481
BssT1I CCWWGG 1 cut(s) 566
Bst2UI CCWGG 3 cut(s) 288, 377, 1166
Bst4CI ACNGT 3 cut(s) 160, 616, 769
BstACI GRCGYC 1 cut(s) 1481
BstBI TTCGAA 1 cut(s) 740
BstC8I GCNNGC 6 cut(s) 134, 168, 188, 901, 1101, 1328
BstDEI CTNAG 5 cut(s) 23, 702, 1412, 1476, 1500
BstDSI CCRYGG 1 cut(s) 1458
BstF5I GGATG 4 cut(s) 13, 588, 1159, 1522
BstFNI CGCG 1 cut(s) 190
BstKTI GATC 9 cut(s) 322, 514, 553, 565, 664, 690, 1126, 1150, 1162
BstMAI GTCTC 3 cut(s) 91, 276, 628
BstMBI GATC 9 cut(s) 319, 511, 550, 562, 661, 687, 1123, 1147, 1159
BstMWI GCNNNNNNNGC 2 cut(s) 707, 1285
BstNI CCWGG 3 cut(s) 288, 377, 1166
BstSCI CCNGG 6 cut(s) 12, 286, 375, 1164, 1229, 1230
BstSFI CTRYAG 1 cut(s) 765
BstSLI GKGCMC 2 cut(s) 621, 1600
BstUI CGCG 1 cut(s) 190
BstV1I GCAGC 4 cut(s) 101, 226, 417, 1387
BstV2I GAAGAC 1 cut(s) 48
BstX2I RGATCY 3 cut(s) 562, 661, 687
BstYI RGATCY 3 cut(s) 562, 661, 687
Bsu36I CCTNAGG 1 cut(s) 1476
BsuRI GGCC 4 cut(s) 147, 339, 1288, 1393
BtgI CCRYGG 1 cut(s) 1458
BtgZI GCGATG 1 cut(s) 257
BtrI CACGTC 1 cut(s) 355
BtsCI GGATG 4 cut(s) 13, 588, 1159, 1522
BtsIMutI CAGTG 5 cut(s) 231, 507, 1104, 1169, 1435
BveI ACCTGC 1 cut(s) 1424
Cac8I GCNNGC 6 cut(s) 134, 168, 188, 901, 1101, 1328
CaiI CAGNNNCTG 1 cut(s) 935
Cfr10I RCCGGY 1 cut(s) 1245
Cfr13I GGNCC 4 cut(s) 145, 338, 1074, 1436
Cfr9I CCCGGG 1 cut(s) 1230
CseI GACGC 1 cut(s) 1470
Csp6I GTAC 4 cut(s) 373, 797, 1018, 1315
CspAI ACCGGT 1 cut(s) 1245
CspCI CAANNNNNGTGG 4 cut(s) 751, 786, 1518, 1553
CviAII CATG 3 cut(s) 436, 1345, 1620
CviQI GTAC 4 cut(s) 373, 797, 1018, 1315
DdeI CTNAG 5 cut(s) 23, 702, 1412, 1476, 1500
DpnI GATC 9 cut(s) 321, 513, 552, 564, 663, 689, 1125, 1149, 1161
DpnII GATC 9 cut(s) 319, 511, 550, 562, 661, 687, 1123, 1147, 1159
DriI GACNNNNNGTC 1 cut(s) 353
Eam1105I GACNNNNNGTC 1 cut(s) 353
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 2 cut(s) 22, 186
Eco130I CCWWGG 1 cut(s) 566
Eco24I GRGCYC 3 cut(s) 24, 188, 427
Eco31I GGTCTC 1 cut(s) 276
Eco47I GGWCC 2 cut(s) 1074, 1436
Eco53kI GAGCTC 2 cut(s) 22, 186
Eco57I CTGAAG 1 cut(s) 255
Eco81I CCTNAGG 1 cut(s) 1476
Eco88I CYCGRG 2 cut(s) 137, 1230
EcoICRI GAGCTC 2 cut(s) 22, 186
EcoO109I RGGNCCY 1 cut(s) 1436
EcoRII CCWGG 3 cut(s) 286, 375, 1164
EcoT14I CCWWGG 1 cut(s) 566
EcoT38I GRGCYC 3 cut(s) 24, 188, 427
ErhI CCWWGG 1 cut(s) 566
Esp3I CGTCTC 2 cut(s) 91, 628
FaeI CATG 3 cut(s) 439, 1348, 1623
FalI AAGNNNNNCTT 4 cut(s) 924, 956, 1023, 1055
FaqI GGGAC 4 cut(s) 29, 110, 188, 1247
FatI CATG 3 cut(s) 435, 1344, 1619
Fnu4HI GCNGC 4 cut(s) 115, 240, 431, 1401
FokI GGATG 4 cut(s) 20, 575, 1166, 1529
FriOI GRGCYC 3 cut(s) 24, 188, 427
Fsp4HI GCNGC 4 cut(s) 115, 240, 431, 1401
FspBI CTAG 3 cut(s) 567, 996, 1100
GluI GCNGC 4 cut(s) 115, 240, 431, 1401
GsuI CTGGAG 1 cut(s) 1044
HaeIII GGCC 4 cut(s) 147, 339, 1288, 1393
HapII CCGG 3 cut(s) 13, 1231, 1246
HgaI GACGC 1 cut(s) 1470
Hin1I GRCGYC 1 cut(s) 1481
Hin1II CATG 3 cut(s) 439, 1348, 1623
HincII GTYRAC 1 cut(s) 491
HindII GTYRAC 1 cut(s) 491
HindIII AAGCTT 1 cut(s) 311
HpaII CCGG 3 cut(s) 13, 1231, 1246
HphI GGTGA 5 cut(s) 658, 878, 1121, 1148, 1154
Hpy166II GTNNAC 3 cut(s) 491, 993, 1598
Hpy188I TCNGA 4 cut(s) 40, 129, 1008, 1501
Hpy188III TCNNGA 6 cut(s) 106, 391, 821, 887, 1028, 1151
Hpy8I GTNNAC 3 cut(s) 491, 993, 1598
Hpy99I CGWCG 1 cut(s) 1275
HpyCH4III ACNGT 3 cut(s) 160, 616, 769
HpyCH4IV ACGT 4 cut(s) 354, 980, 1113, 1273
HpyF10VI GCNNNNNNNGC 2 cut(s) 707, 1285
HpyF3I CTNAG 5 cut(s) 23, 702, 1412, 1476, 1500
HpySE526I ACGT 4 cut(s) 354, 980, 1113, 1273
Hsp92I GRCGYC 1 cut(s) 1481
Hsp92II CATG 3 cut(s) 439, 1348, 1623
Kzo9I GATC 9 cut(s) 319, 511, 550, 562, 661, 687, 1123, 1147, 1159
LmnI GCTCC 3 cut(s) 430, 586, 1043
Lsp1109I GCAGC 4 cut(s) 101, 226, 417, 1387
LweI GCATC 2 cut(s) 763, 1324
MaeI CTAG 3 cut(s) 567, 996, 1100
MaeII ACGT 4 cut(s) 354, 980, 1113, 1273
MaeIII GTNAC 2 cut(s) 1109, 1136
MalI GATC 9 cut(s) 321, 513, 552, 564, 663, 689, 1125, 1149, 1161
MboI GATC 9 cut(s) 319, 511, 550, 562, 661, 687, 1123, 1147, 1159
MboII GAAGA 7 cut(s) 53, 217, 697, 791, 881, 890, 1560
MflI RGATCY 3 cut(s) 562, 661, 687
MhlI GDGCHC 6 cut(s) 24, 188, 427, 583, 621, 1600
MluCI AATT 6 cut(s) 447, 735, 950, 1141, 1194, 1603
MlyI GAGTC 2 cut(s) 92, 1437
MmeI TCCRAC 3 cut(s) 620, 956, 1509
MroXI GAANNNNTTC 2 cut(s) 923, 1035
MseI TTAA 8 cut(s) 402, 645, 653, 789, 942, 1242, 1293, 1581
MslI CAYNNNNRTG 1 cut(s) 1134
MspA1I CMGCKG 3 cut(s) 430, 1134, 1400
MspI CCGG 3 cut(s) 13, 1231, 1246
MspR9I CCNGG 6 cut(s) 14, 288, 377, 1166, 1231, 1232
Mva1269I GAATGC 2 cut(s) 331, 1319
MvaI CCWGG 3 cut(s) 288, 377, 1166
MvnI CGCG 1 cut(s) 190
MwoI GCNNNNNNNGC 2 cut(s) 707, 1285
NciI CCSGG 3 cut(s) 14, 1231, 1232
NdeII GATC 9 cut(s) 319, 511, 550, 562, 661, 687, 1123, 1147, 1159
NheI GCTAGC 1 cut(s) 1099
NlaIII CATG 3 cut(s) 439, 1348, 1623
NlaIV GGNNCC 4 cut(s) 52, 146, 426, 1362
NmeAIII GCCGAG 1 cut(s) 301
NmuCI GTSAC 2 cut(s) 1109, 1136
NspV TTCGAA 1 cut(s) 740
OliI CACNNNNGTG 1 cut(s) 1134
PcsI WCGNNNNNNNCGW 4 cut(s) 24, 51, 1276, 1323
PctI GAATGC 2 cut(s) 331, 1319
PdmI GAANNNNTTC 2 cut(s) 923, 1035
PfeI GAWTC 8 cut(s) 208, 259, 758, 782, 836, 854, 1565, 1586
PflMI CCANNNNNTGG 1 cut(s) 162
PinAI ACCGGT 1 cut(s) 1245
PkrI GCNGC 4 cut(s) 116, 241, 432, 1402
PleI GAGTC 2 cut(s) 92, 1437
PpsI GAGTC 2 cut(s) 92, 1437
PpuMI RGGWCCY 1 cut(s) 1436
PshBI ATTAAT 1 cut(s) 789
Psp124BI GAGCTC 2 cut(s) 24, 188
Psp5II RGGWCCY 1 cut(s) 1436
Psp6I CCWGG 3 cut(s) 286, 375, 1164
PspGI CCWGG 3 cut(s) 286, 375, 1164
PspN4I GGNNCC 4 cut(s) 52, 146, 426, 1362
PspPI GGNCC 4 cut(s) 145, 338, 1074, 1436
PspPPI RGGWCCY 1 cut(s) 1436
PstNI CAGNNNCTG 1 cut(s) 935
PsuI RGATCY 3 cut(s) 562, 661, 687
PvuII CAGCTG 1 cut(s) 1400
RsaI GTAC 4 cut(s) 374, 798, 1019, 1316
RsaNI GTAC 4 cut(s) 373, 797, 1018, 1315
RseI CAYNNNNRTG 1 cut(s) 1134
SacI GAGCTC 2 cut(s) 24, 188
SaqAI TTAA 8 cut(s) 402, 645, 653, 789, 942, 1242, 1293, 1581
SatI GCNGC 4 cut(s) 115, 240, 431, 1401
Sau3AI GATC 9 cut(s) 319, 511, 550, 562, 661, 687, 1123, 1147, 1159
Sau96I GGNCC 4 cut(s) 145, 338, 1074, 1436
SchI GAGTC 2 cut(s) 92, 1437
ScrFI CCNGG 6 cut(s) 14, 288, 377, 1166, 1231, 1232
SduI GDGCHC 6 cut(s) 24, 188, 427, 583, 621, 1600
SfaNI GCATC 2 cut(s) 763, 1324
SfcI CTRYAG 1 cut(s) 765
SfuI TTCGAA 1 cut(s) 740
SinI GGWCC 2 cut(s) 1074, 1436
SmaI CCCGGG 1 cut(s) 1232
SmiMI CAYNNNNRTG 1 cut(s) 1134
SmlI CTYRAG 3 cut(s) 714, 885, 1448
SmoI CTYRAG 3 cut(s) 714, 885, 1448
SpeI ACTAGT 1 cut(s) 995
Sse9I AATT 6 cut(s) 447, 735, 950, 1141, 1194, 1603
SsiI CCGC 4 cut(s) 5, 428, 776, 1134
SspMI CTAG 3 cut(s) 567, 996, 1100
SstI GAGCTC 2 cut(s) 24, 188
StyD4I CCNGG 6 cut(s) 12, 286, 375, 1164, 1229, 1230
StyI CCWWGG 1 cut(s) 566
TaaI ACNGT 3 cut(s) 160, 616, 769
TaiI ACGT 4 cut(s) 357, 983, 1116, 1276
TaqI TCGA 3 cut(s) 740, 1270, 1441
TasI AATT 6 cut(s) 447, 735, 950, 1141, 1194, 1603
TatI WGTACW 2 cut(s) 796, 1017
TfiI GAWTC 8 cut(s) 208, 259, 758, 782, 836, 854, 1565, 1586
Tru1I TTAA 8 cut(s) 402, 645, 653, 789, 942, 1242, 1293, 1581
Tru9I TTAA 8 cut(s) 402, 645, 653, 789, 942, 1242, 1293, 1581
TscAI CASTG 5 cut(s) 238, 514, 1111, 1176, 1435
TseFI GTSAC 2 cut(s) 1109, 1136
TseI GCWGC 4 cut(s) 114, 239, 430, 1400
Tsp45I GTSAC 2 cut(s) 1109, 1136
TspDTI ATGAA 5 cut(s) 446, 807, 867, 1578, 1596
TspGWI ACGGA 4 cut(s) 672, 680, 1322, 1447
TspMI CCCGGG 1 cut(s) 1230
TspRI CASTG 5 cut(s) 238, 514, 1111, 1176, 1435
Van91I CCANNNNNTGG 1 cut(s) 162
VneI GTGCAC 1 cut(s) 1596
VpaK11BI GGWCC 2 cut(s) 1074, 1436
VspI ATTAAT 1 cut(s) 789
XapI RAATTY 3 cut(s) 447, 735, 1603
XcmI CCANNNNNNNNNTGG 1 cut(s) 1068
XmaI CCCGGG 1 cut(s) 1230
XmaJI CCTAGG 1 cut(s) 566
XmnI GAANNNNTTC 2 cut(s) 923, 1035
XspI CTAG 3 cut(s) 567, 996, 1100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.