AT3G53490

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014506
Physical Location & Seq
Forward (+)
19832448 .. 19834304
1857 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G53490.1

Sequence Viewer

Length: 678 bp
ATGTCAATCCAAATTCCGTCAGCTTCTTGGGATTTTCGCGGTTTCCTACTCTGTTTCATCTTCTTCAGCTCGTTACCAGGAACAATACTAACACAAGAAGTTACATTAGATTCGGTTCAGATTTTCACGACTCACGATTGGTTCTCGACTAAGCCTACAGTCTTTTTCCAATGCAAAGGAGAGAACAAGACGGTGTTGCCTGATGTGAAGAGAACCAATGTTTCTTACTCTTTCAATGGCGAAGAATCTTGGCAGCCACTAACCGAACTTCAAGGAACAAAATGCAAGAGATGTGGAATCTATGAGGACGACCCTCTTAAATATGATACATTTGACGAATGGGAGCTTTGCCCTTCTGATTTCACAGCAGAGGGTAGTTACAAGCGCTTCAAAGAGAAAGAATTTAATGCTACTTTTCTCTGCCATGGATGCTCACAAGTTGGAGCTGGTTCGAATAAAGAATCAGGCACCGAGAAGGAAGAACAAAAGGGCGGAATGCATCCTGGAATTGTGGTACTGATTGTAGTACTCTTGTTAGGTGTAGTTGCGGTGGGACTCTTGGTAGGTTACAAGTATTGGAGAAAGAAGAAACGGCAACAAGAGCAGGCCCGGTTTCTCAAGCTGTTCGAAGATGGTGACGATATTGAGGACGAACTTGGCCTTGAAAATACCCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

225

Amino Acids

25.6

Weight (kDa)

5.04

Isoelectric Point (pI)

37.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7953 PF25829 32 - 142 5.8e-54 Domain of unknown function (DUF7953)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014506)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53490
fragaria_vesca FvH4_6g14800
malus_domestica MD04G1110000.v1.1 MD12G1131500.v1.1
prunus_persica Prupe.6G245800_v2.0.a1
pyrus_communis pycom04g10600 pycom12g12720
rosa_chinensis RchiOBHm_Chr3g0468021
rosa_laevigata RLG00000024458
rosa_multiflora Rmu_sc0006706.1_g000009
rosa_roxburghii Rroxscaffold_6G00413170
rosa_rugosa Rorug03G0091500
rosa_samantha Rh3AG140200 Rh3BG161400 Rh3CG161500 Rh3DG162000
rosa_wichuraiana Rw3G013240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 467
AccII CGCG 1 cut(s) 39
AciI CCGC 3 cut(s) 39, 492, 548
AcsI RAATTY 2 cut(s) 12, 401
AcuI CTGAAG 1 cut(s) 49
AfaI GTAC 2 cut(s) 516, 528
AfeI AGCGCT 1 cut(s) 386
AgsI TTSAA 4 cut(s) 235, 272, 391, 665
AjnI CCWGG 2 cut(s) 76, 502
AluBI AGCT 5 cut(s) 23, 69, 346, 446, 622
AluI AGCT 5 cut(s) 23, 69, 346, 446, 622
Aor51HI AGCGCT 1 cut(s) 386
AoxI GGCC 2 cut(s) 606, 658
ApeKI GCWGC 1 cut(s) 253
ApoI RAATTY 2 cut(s) 12, 401
AspLEI GCGC 1 cut(s) 387
AspS9I GGNCC 1 cut(s) 607
AsuC2I CCSGG 1 cut(s) 610
AsuHPI GGTGA 1 cut(s) 647
AsuII TTCGAA 2 cut(s) 452, 627
BanI GGYRCC 1 cut(s) 467
BbvI GCAGC 1 cut(s) 265
BccI CCATC 1 cut(s) 626
BceAI ACGGC 1 cut(s) 608
BciT130I CCWGG 2 cut(s) 78, 504
BcnI CCSGG 1 cut(s) 610
BfmI CTRYAG 1 cut(s) 156
BfoI RGCGCY 1 cut(s) 388
BisI GCNGC 1 cut(s) 254
BlsI GCNGC 1 cut(s) 255
BmcAI AGTACT 1 cut(s) 528
Bme1390I CCNGG 3 cut(s) 78, 504, 610
BmgT120I GGNCC 1 cut(s) 607
BmiI GGNNCC 1 cut(s) 469
BmrFI CCNGG 3 cut(s) 78, 504, 610
BmsI GCATC 2 cut(s) 419, 508
Bpu14I TTCGAA 2 cut(s) 452, 627
BpuEI CTTGAG 1 cut(s) 602
BpuMI CCSGG 1 cut(s) 610
BsaJI CCNNGG 1 cut(s) 424
BseBI CCWGG 2 cut(s) 78, 504
BseDI CCNNGG 1 cut(s) 424
BseGI GGATG 2 cut(s) 434, 499
BseXI GCAGC 1 cut(s) 265
Bsh1236I CGCG 1 cut(s) 39
BshFI GGCC 2 cut(s) 608, 660
BshNI GGYRCC 1 cut(s) 467
BsiSI CCGG 1 cut(s) 610
BslFI GGGAC 1 cut(s) 567
BsmFI GGGAC 1 cut(s) 567
BsmI GAATGC 1 cut(s) 501
BsnI GGCC 2 cut(s) 608, 660
Bsp119I TTCGAA 2 cut(s) 452, 627
Bsp19I CCATGG 1 cut(s) 424
BspACI CCGC 3 cut(s) 39, 492, 548
BspANI GGCC 2 cut(s) 608, 660
BspFNI CGCG 1 cut(s) 39
BspLI GGNNCC 1 cut(s) 469
BspT104I TTCGAA 2 cut(s) 452, 627
BspT107I GGYRCC 1 cut(s) 467
BssECI CCNNGG 1 cut(s) 424
BssT1I CCWWGG 1 cut(s) 424
Bst2UI CCWGG 2 cut(s) 78, 504
Bst4CI ACNGT 2 cut(s) 160, 193
Bst6I CTCTTC 1 cut(s) 203
BstBI TTCGAA 2 cut(s) 452, 627
BstC8I GCNNGC 1 cut(s) 606
BstDEI CTNAG 1 cut(s) 150
BstDSI CCRYGG 1 cut(s) 424
BstF5I GGATG 2 cut(s) 434, 499
BstFNI CGCG 1 cut(s) 39
BstH2I RGCGCY 1 cut(s) 388
BstHHI GCGC 1 cut(s) 387
BstMWI GCNNNNNNNGC 2 cut(s) 429, 601
BstNI CCWGG 2 cut(s) 78, 504
BstSCI CCNGG 3 cut(s) 76, 502, 608
BstSFI CTRYAG 1 cut(s) 156
BstUI CGCG 1 cut(s) 39
BstV1I GCAGC 1 cut(s) 265
BsuRI GGCC 2 cut(s) 608, 660
BtgI CCRYGG 1 cut(s) 424
BtsCI GGATG 2 cut(s) 434, 499
Cac8I GCNNGC 1 cut(s) 606
CfoI GCGC 1 cut(s) 387
Cfr13I GGNCC 1 cut(s) 607
Csp6I GTAC 2 cut(s) 515, 527
CspCI CAANNNNNGTGG 2 cut(s) 274, 309
CviAII CATG 1 cut(s) 425
CviJI RGCY 9 cut(s) 23, 69, 154, 256, 346, 446, 608, 622, 660
CviKI_1 RGCY 9 cut(s) 23, 69, 154, 256, 346, 446, 608, 622, 660
CviQI GTAC 2 cut(s) 515, 527
DdeI CTNAG 1 cut(s) 150
Eam1104I CTCTTC 1 cut(s) 203
EarI CTCTTC 1 cut(s) 203
EciI GGCGGA 1 cut(s) 507
Eco130I CCWWGG 1 cut(s) 424
Eco47III AGCGCT 1 cut(s) 386
Eco57I CTGAAG 1 cut(s) 49
EcoRII CCWGG 2 cut(s) 76, 502
EcoT14I CCWWGG 1 cut(s) 424
EcoT22I ATGCAT 1 cut(s) 501
ErhI CCWWGG 1 cut(s) 424
FaeI CATG 1 cut(s) 428
FaiI YATR 4 cut(s) 303, 324, 426, 676
FaqI GGGAC 1 cut(s) 567
FatI CATG 1 cut(s) 424
Fnu4HI GCNGC 1 cut(s) 254
FokI GGATG 2 cut(s) 441, 486
Fsp4HI GCNGC 1 cut(s) 254
GlaI GCGC 1 cut(s) 386
GluI GCNGC 1 cut(s) 254
HaeII RGCGCY 1 cut(s) 388
HaeIII GGCC 2 cut(s) 608, 660
HapII CCGG 1 cut(s) 610
HhaI GCGC 1 cut(s) 387
Hin1II CATG 1 cut(s) 428
Hin6I GCGC 1 cut(s) 385
HinP1I GCGC 1 cut(s) 385
HinfI GANTC 6 cut(s) 110, 130, 245, 297, 461, 555
HpaII CCGG 1 cut(s) 610
HphI GGTGA 1 cut(s) 647
Hpy188I TCNGA 2 cut(s) 120, 358
Hpy188III TCNNGA 3 cut(s) 127, 134, 145
HpyAV CCTTC 2 cut(s) 363, 469
HpyCH4III ACNGT 2 cut(s) 160, 193
HpyCH4V TGCA 3 cut(s) 174, 285, 499
HpyF10VI GCNNNNNNNGC 2 cut(s) 429, 601
HpyF3I CTNAG 1 cut(s) 150
Hsp92II CATG 1 cut(s) 428
HspAI GCGC 1 cut(s) 385
LmnI GCTCC 2 cut(s) 343, 443
LpnPI CCDG 9 cut(s) 63, 90, 213, 432, 450, 489, 516, 590, 623
Lsp1109I GCAGC 1 cut(s) 265
LweI GCATC 2 cut(s) 419, 508
MaeIII GTNAC 5 cut(s) 72, 100, 377, 566, 635
MboII GAAGA 7 cut(s) 52, 55, 220, 254, 491, 598, 641
MluCI AATT 3 cut(s) 12, 401, 507
MlyI GAGTC 2 cut(s) 124, 549
MmeI TCCRAC 1 cut(s) 421
MnlI CCTC 4 cut(s) 298, 324, 364, 640
Mph1103I ATGCAT 1 cut(s) 501
MseI TTAA 2 cut(s) 318, 405
MspI CCGG 1 cut(s) 610
MspR9I CCNGG 3 cut(s) 78, 504, 610
Mva1269I GAATGC 1 cut(s) 501
MvaI CCWGG 2 cut(s) 78, 504
MvnI CGCG 1 cut(s) 39
MwoI GCNNNNNNNGC 2 cut(s) 429, 601
NciI CCSGG 1 cut(s) 610
NcoI CCATGG 1 cut(s) 424
NlaIII CATG 1 cut(s) 428
NlaIV GGNNCC 1 cut(s) 469
NmuCI GTSAC 1 cut(s) 635
NsiI ATGCAT 1 cut(s) 501
NspV TTCGAA 2 cut(s) 452, 627
PctI GAATGC 1 cut(s) 501
PfeI GAWTC 4 cut(s) 110, 245, 297, 461
PfoI TCCNGGA 1 cut(s) 502
PkrI GCNGC 1 cut(s) 255
PleI GAGTC 2 cut(s) 124, 549
PpsI GAGTC 2 cut(s) 124, 549
Psp6I CCWGG 2 cut(s) 76, 502
PspGI CCWGG 2 cut(s) 76, 502
PspN4I GGNNCC 1 cut(s) 469
PspPI GGNCC 1 cut(s) 607
RsaI GTAC 2 cut(s) 516, 528
RsaNI GTAC 2 cut(s) 515, 527
SaqAI TTAA 2 cut(s) 318, 405
SatI GCNGC 1 cut(s) 254
Sau96I GGNCC 1 cut(s) 607
ScaI AGTACT 1 cut(s) 528
SchI GAGTC 2 cut(s) 124, 549
ScrFI CCNGG 3 cut(s) 78, 504, 610
SetI ASST 7 cut(s) 25, 71, 348, 448, 541, 568, 624
SfaNI GCATC 2 cut(s) 419, 508
SfcI CTRYAG 1 cut(s) 156
SfuI TTCGAA 2 cut(s) 452, 627
SmlI CTYRAG 1 cut(s) 617
SmoI CTYRAG 1 cut(s) 617
Sse9I AATT 3 cut(s) 12, 401, 507
SsiI CCGC 3 cut(s) 39, 492, 548
StyD4I CCNGG 3 cut(s) 76, 502, 608
StyI CCWWGG 1 cut(s) 424
TaaI ACNGT 2 cut(s) 160, 193
TaqI TCGA 3 cut(s) 146, 452, 627
TasI AATT 3 cut(s) 12, 401, 507
TatI WGTACW 1 cut(s) 526
TfiI GAWTC 4 cut(s) 110, 245, 297, 461
Tru1I TTAA 2 cut(s) 318, 405
Tru9I TTAA 2 cut(s) 318, 405
TseFI GTSAC 1 cut(s) 635
TseI GCWGC 1 cut(s) 253
Tsp45I GTSAC 1 cut(s) 635
TspDTI ATGAA 1 cut(s) 46
TspGWI ACGGA 1 cut(s) 6
XapI RAATTY 2 cut(s) 12, 401
ZrmI AGTACT 1 cut(s) 528
Zsp2I ATGCAT 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.