MD12G1131500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014506
Physical Location & Seq
Forward (+)
20665007 .. 20667076
2070 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1131500.v1.1.491

Sequence Viewer

Length: 687 bp
ATGTCGATTCGGGAGCTGGGGAGCTCTAGGGTTTCGGTCAATTTGTTCACCTTCTGCTTTTTCCTCAGCTGCTTTCCAGGGCTTATATTATCGGCGATCGTGACGCTGGATTCGATCGTGATATACAACACGCACGAATTGATAAAGGCGGTGAAACCAAGGGTGTATTTCGAATGCAGAGGGGAGAACAAGACGGAGTTTCCAGACGTGAAGGAGAAGAACGTCACGTATAAATTCAACGGCGAAGAGTCTTGGCAGCCACTGACAGAGTTTTCGGGTAAGAAGTGCAAGCGATGTGGATTGTACGAGGCGGATACCTTTAAACCAGATGACAAATTTGATGAGTGGGAGTTGTGTCCTTCTGACTTCAATGCTTCTGATGGAAAATACGTCCGGTTCAATAACAAGGAATTCAACCTTACCTTTTGGTGCCCAAAATGTGTACCTGGTTCACCTGCTTCCAATTCCCCTACTGCGCCGCATAAAGAAGAAAAGGAGAAGGGAATGCATATTGTTCTAATACTTTTAATCGTTGTAGCCGTTTTAGCATTTTGTATTGTCGTTGGCTTTGTTGCATACAAGTACTGGCAAAAGAAGAAGAGAGAGCACGATCAGGCTCGGTTCCTAAAGCTGTTTGAAGACGGGGATGATATTGAAGATGAAATGGGGCTTGACCATATAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

229

Amino Acids

26.28

Weight (kDa)

5.81

Isoelectric Point (pI)

40.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7953 PF25829 31 - 145 1.2e-54 Domain of unknown function (DUF7953)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014506)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53490
fragaria_vesca FvH4_6g14800
malus_domestica MD04G1110000.v1.1 MD12G1131500.v1.1
prunus_persica Prupe.6G245800_v2.0.a1
pyrus_communis pycom04g10600 pycom12g12720
rosa_chinensis RchiOBHm_Chr3g0468021
rosa_laevigata RLG00000024458
rosa_multiflora Rmu_sc0006706.1_g000009
rosa_roxburghii Rroxscaffold_6G00413170
rosa_rugosa Rorug03G0091500
rosa_samantha Rh3AG140200 Rh3BG161400 Rh3CG161500 Rh3DG162000
rosa_wichuraiana Rw3G013240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 463
Acc36I ACCTGC 1 cut(s) 463
AccB1I GGYRCC 1 cut(s) 429
AciI CCGC 3 cut(s) 149, 311, 479
AcsI RAATTY 3 cut(s) 233, 335, 410
AfaI GTAC 3 cut(s) 305, 444, 584
AgsI TTSAA 6 cut(s) 238, 370, 400, 415, 638, 656
AjiI CACGTC 1 cut(s) 208
AjnI CCWGG 2 cut(s) 76, 445
AluBI AGCT 4 cut(s) 16, 24, 69, 631
AluI AGCT 4 cut(s) 16, 24, 69, 631
Alw21I GWGCWC 2 cut(s) 26, 609
AlwNI CAGNNNCTG 1 cut(s) 262
ApeKI GCWGC 2 cut(s) 69, 256
ApoI RAATTY 3 cut(s) 233, 335, 410
ArsI GACNNNNNNTTYG 4 cut(s) 94, 126, 256, 288
AspLEI GCGC 1 cut(s) 478
AsuHPI GGTGA 3 cut(s) 40, 163, 444
AsuII TTCGAA 1 cut(s) 171
BaeGI GKGCMC 1 cut(s) 434
BanI GGYRCC 1 cut(s) 429
BanII GRGCYC 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 645
Bbv12I GWGCWC 2 cut(s) 26, 609
BbvCI CCTCAGC 1 cut(s) 65
BbvI GCAGC 2 cut(s) 56, 268
BccI CCATC 1 cut(s) 374
BceAI ACGGC 2 cut(s) 256, 524
BciT130I CCWGG 2 cut(s) 78, 447
BciVI GTATCC 1 cut(s) 307
BfaI CTAG 1 cut(s) 27
BfuAI ACCTGC 1 cut(s) 463
BfuI GTATCC 1 cut(s) 307
BisI GCNGC 3 cut(s) 70, 257, 479
BlsI GCNGC 3 cut(s) 71, 258, 480
BmcAI AGTACT 1 cut(s) 584
Bme1390I CCNGG 2 cut(s) 78, 447
BmgBI CACGTC 1 cut(s) 208
BmiI GGNNCC 2 cut(s) 431, 623
BmrFI CCNGG 2 cut(s) 78, 447
BpiI GAAGAC 1 cut(s) 645
Bpu10I CCTNAGC 1 cut(s) 65
Bpu14I TTCGAA 1 cut(s) 171
BsaAI YACGTR 1 cut(s) 228
BsaJI CCNNGG 2 cut(s) 77, 158
BsaWI WCCGGW 1 cut(s) 393
Bse1I ACTGG 1 cut(s) 590
BseBI CCWGG 2 cut(s) 78, 447
BseDI CCNNGG 2 cut(s) 77, 158
BseGI GGATG 1 cut(s) 652
BseMII CTCAG 1 cut(s) 79
BseNI ACTGG 1 cut(s) 590
BseSI GKGCMC 1 cut(s) 434
BseXI GCAGC 2 cut(s) 56, 268
BseYI CCCAGC 1 cut(s) 16
Bsh1285I CGRYCG 2 cut(s) 99, 117
BshNI GGYRCC 1 cut(s) 429
BsiEI CGRYCG 2 cut(s) 99, 117
BsiHKAI GWGCWC 2 cut(s) 26, 609
BsiSI CCGG 1 cut(s) 394
BsmI GAATGC 2 cut(s) 179, 510
Bsp119I TTCGAA 1 cut(s) 171
Bsp1286I GDGCHC 3 cut(s) 26, 434, 609
Bsp143I GATC 3 cut(s) 96, 114, 610
BspACI CCGC 3 cut(s) 149, 311, 479
BspCNI CTCAG 1 cut(s) 78
BspLI GGNNCC 2 cut(s) 431, 623
BspMI ACCTGC 1 cut(s) 463
BspT104I TTCGAA 1 cut(s) 171
BspT107I GGYRCC 1 cut(s) 429
BsrI ACTGG 1 cut(s) 590
BssECI CCNNGG 2 cut(s) 77, 158
BssMI GATC 3 cut(s) 96, 114, 610
BssT1I CCWWGG 1 cut(s) 158
Bst2UI CCWGG 2 cut(s) 78, 447
Bst6I CTCTTC 2 cut(s) 240, 593
BstBAI YACGTR 1 cut(s) 228
BstBI TTCGAA 1 cut(s) 171
BstC8I GCNNGC 1 cut(s) 290
BstDEI CTNAG 1 cut(s) 65
BstF5I GGATG 1 cut(s) 652
BstHHI GCGC 1 cut(s) 478
BstKTI GATC 3 cut(s) 99, 117, 613
BstMBI GATC 3 cut(s) 96, 114, 610
BstMCI CGRYCG 2 cut(s) 99, 117
BstMWI GCNNNNNNNGC 1 cut(s) 545
BstNI CCWGG 2 cut(s) 78, 447
BstSCI CCNGG 2 cut(s) 76, 445
BstSLI GKGCMC 1 cut(s) 434
BstV1I GCAGC 2 cut(s) 56, 268
BstV2I GAAGAC 1 cut(s) 645
BsuI GTATCC 1 cut(s) 307
BtgZI GCGATG 1 cut(s) 307
BtrI CACGTC 1 cut(s) 208
BtsCI GGATG 1 cut(s) 652
BtsIMutI CAGTG 1 cut(s) 260
BveI ACCTGC 1 cut(s) 463
Cac8I GCNNGC 1 cut(s) 290
CaiI CAGNNNCTG 1 cut(s) 262
CfoI GCGC 1 cut(s) 478
CseI GACGC 1 cut(s) 112
CsiI ACCWGGT 1 cut(s) 445
Csp6I GTAC 3 cut(s) 304, 443, 583
CspCI CAANNNNNGTGG 2 cut(s) 277, 312
CviQI GTAC 3 cut(s) 304, 443, 583
DdeI CTNAG 1 cut(s) 65
DpnI GATC 3 cut(s) 98, 116, 612
DpnII GATC 3 cut(s) 96, 114, 610
DraI TTTAAA 1 cut(s) 322
Eam1104I CTCTTC 2 cut(s) 240, 593
EarI CTCTTC 2 cut(s) 240, 593
EciI GGCGGA 1 cut(s) 326
Ecl136II GAGCTC 1 cut(s) 24
Eco130I CCWWGG 1 cut(s) 158
Eco24I GRGCYC 1 cut(s) 26
Eco53kI GAGCTC 1 cut(s) 24
EcoICRI GAGCTC 1 cut(s) 24
EcoRI GAATTC 1 cut(s) 410
EcoRII CCWGG 2 cut(s) 76, 445
EcoT14I CCWWGG 1 cut(s) 158
EcoT22I ATGCAT 1 cut(s) 510
EcoT38I GRGCYC 1 cut(s) 26
ErhI CCWWGG 1 cut(s) 158
FaiI YATR 8 cut(s) 86, 124, 231, 483, 510, 577, 678, 680
Fnu4HI GCNGC 3 cut(s) 70, 257, 479
FokI GGATG 1 cut(s) 659
FriOI GRGCYC 1 cut(s) 26
Fsp4HI GCNGC 3 cut(s) 70, 257, 479
FspBI CTAG 1 cut(s) 27
GlaI GCGC 1 cut(s) 477
GluI GCNGC 3 cut(s) 70, 257, 479
GsaI CCCAGC 1 cut(s) 20
HapII CCGG 1 cut(s) 394
HgaI GACGC 1 cut(s) 112
HhaI GCGC 1 cut(s) 478
Hin6I GCGC 1 cut(s) 476
HinP1I GCGC 1 cut(s) 476
HinfI GANTC 3 cut(s) 7, 110, 248
HpaII CCGG 1 cut(s) 394
HphI GGTGA 3 cut(s) 40, 163, 444
Hpy166II GTNNAC 3 cut(s) 48, 443, 452
Hpy188I TCNGA 3 cut(s) 364, 379, 686
Hpy188III TCNNGA 4 cut(s) 11, 100, 118, 203
Hpy8I GTNNAC 3 cut(s) 48, 443, 452
HpyAV CCTTC 4 cut(s) 61, 205, 369, 493
HpyCH4IV ACGT 4 cut(s) 207, 222, 227, 390
HpyCH4V TGCA 4 cut(s) 177, 288, 508, 575
HpyF10VI GCNNNNNNNGC 1 cut(s) 545
HpyF3I CTNAG 1 cut(s) 65
HpySE526I ACGT 4 cut(s) 207, 222, 227, 390
HspAI GCGC 1 cut(s) 476
Kzo9I GATC 3 cut(s) 96, 114, 610
LmnI GCTCC 2 cut(s) 13, 21
Lsp1109I GCAGC 2 cut(s) 56, 268
MabI ACCWGGT 1 cut(s) 445
MaeI CTAG 1 cut(s) 27
MaeII ACGT 4 cut(s) 207, 222, 227, 390
MaeIII GTNAC 2 cut(s) 100, 223
MalI GATC 3 cut(s) 98, 116, 612
MboI GATC 3 cut(s) 96, 114, 610
MboII GAAGA 7 cut(s) 229, 257, 500, 607, 610, 650, 668
MhlI GDGCHC 3 cut(s) 26, 434, 609
MluCI AATT 6 cut(s) 40, 137, 233, 335, 410, 463
MlyI GAGTC 1 cut(s) 257
MnlI CCTC 3 cut(s) 74, 173, 301
Mph1103I ATGCAT 1 cut(s) 510
MseI TTAA 2 cut(s) 321, 527
MspA1I CMGCKG 1 cut(s) 69
MspI CCGG 1 cut(s) 394
MspR9I CCNGG 2 cut(s) 78, 447
Mva1269I GAATGC 2 cut(s) 179, 510
MvaI CCWGG 2 cut(s) 78, 447
MwoI GCNNNNNNNGC 1 cut(s) 545
NdeII GATC 3 cut(s) 96, 114, 610
NlaIV GGNNCC 2 cut(s) 431, 623
NmuCI GTSAC 2 cut(s) 100, 223
NsiI ATGCAT 1 cut(s) 510
NspV TTCGAA 1 cut(s) 171
PaqCI CACCTGC 1 cut(s) 463
PcsI WCGNNNNNNNCGW 2 cut(s) 110, 537
PctI GAATGC 2 cut(s) 179, 510
PfeI GAWTC 2 cut(s) 7, 110
PkrI GCNGC 3 cut(s) 71, 258, 480
Ple19I CGATCG 2 cut(s) 99, 117
PleI GAGTC 1 cut(s) 256
PpsI GAGTC 1 cut(s) 256
Ppu21I YACGTR 1 cut(s) 228
Psp124BI GAGCTC 1 cut(s) 26
Psp6I CCWGG 2 cut(s) 76, 445
PspFI CCCAGC 1 cut(s) 16
PspGI CCWGG 2 cut(s) 76, 445
PspN4I GGNNCC 2 cut(s) 431, 623
PstNI CAGNNNCTG 1 cut(s) 262
PvuI CGATCG 2 cut(s) 99, 117
PvuII CAGCTG 1 cut(s) 69
RsaI GTAC 3 cut(s) 305, 444, 584
RsaNI GTAC 3 cut(s) 304, 443, 583
SacI GAGCTC 1 cut(s) 26
SaqAI TTAA 2 cut(s) 321, 527
SatI GCNGC 3 cut(s) 70, 257, 479
Sau3AI GATC 3 cut(s) 96, 114, 610
ScaI AGTACT 1 cut(s) 584
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 2 cut(s) 78, 447
SduI GDGCHC 3 cut(s) 26, 434, 609
SexAI ACCWGGT 1 cut(s) 445
SfuI TTCGAA 1 cut(s) 171
Sse9I AATT 6 cut(s) 40, 137, 233, 335, 410, 463
SsiI CCGC 3 cut(s) 149, 311, 479
SspMI CTAG 1 cut(s) 27
SstI GAGCTC 1 cut(s) 26
StyD4I CCNGG 2 cut(s) 76, 445
StyI CCWWGG 1 cut(s) 158
TaiI ACGT 4 cut(s) 210, 225, 230, 393
TaqI TCGA 3 cut(s) 5, 113, 171
TaqII GACCGA 1 cut(s) 25
TasI AATT 6 cut(s) 40, 137, 233, 335, 410, 463
TatI WGTACW 1 cut(s) 582
TauI GCSGC 1 cut(s) 481
TfiI GAWTC 2 cut(s) 7, 110
Tru1I TTAA 2 cut(s) 321, 527
Tru9I TTAA 2 cut(s) 321, 527
TscAI CASTG 1 cut(s) 267
TseFI GTSAC 2 cut(s) 100, 223
TseI GCWGC 2 cut(s) 69, 256
Tsp45I GTSAC 2 cut(s) 100, 223
TspDTI ATGAA 1 cut(s) 675
TspGWI ACGGA 1 cut(s) 209
TspRI CASTG 1 cut(s) 267
XapI RAATTY 3 cut(s) 233, 335, 410
XspI CTAG 1 cut(s) 27
ZrmI AGTACT 1 cut(s) 584
Zsp2I ATGCAT 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.