MD04G1110000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0014506
Physical Location & Seq
Reverse (-)
19461742 .. 19464288
2547 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1110000.v1.1.491

Sequence Viewer

Length: 687 bp
ATGTCGATAAGGGAGCTGCGGAGCTCTAGTGTTTCCGTCAATTTGTTCACCTTCTGCGTTTTCCTCAGCTGCTTTCCAGGATTTATATTATCGGCGATCGTGACGCTGGATTCGATCGTGATATACAACACGCATGAATTGATAAAGGCGGTGAAACCGAAGGTGTATTTCGAATGCAAGGAGGAGAACAAGACGAATCTTCCAGATGTGAAGGAGAAGAACGTCAGGTATAATTTCAACGGCCAAGAGTCTTGGCAGCCTCTGACAGAGTTTTCGGGTAAGAAGTGCAAGCGATGTGGATTGTATGAGGCGGATACCCTTAAATCGGATGACAAATTCGATGAGTGGGAATTGTGTCCTTCTGACTTCAATGCTTCTGATGGGAAATATGTGCGGTTCAAGAACAAGGAATTCAACGTTACGTTTTGGTGCCCAAAGTGTGTACCTGATACACCTGCTTCCATTTCCACCACTGCGCTGGGCAAAGAAGAAAAAGAGAAGGGAATGCATGTTGTTCTAATACTTTTAATCATTGTAGCCGTTTTAGCAGTGTGTATTGTCGTTGGCTTTGCTGCATACAAGTACTGGCAAAAGAAGAAGAGAGAGCAAGATCAGGCTCGGTTCCTAAAACTGTTTGAAGACGGGGATGGTATTGAAGATGAAATGGGGCTTGACCATATAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

229

Amino Acids

26.13

Weight (kDa)

6.01

Isoelectric Point (pI)

34.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7953 PF25829 31 - 145 5.2e-56 Domain of unknown function (DUF7953)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014506)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53490
fragaria_vesca FvH4_6g14800
malus_domestica MD04G1110000.v1.1 MD12G1131500.v1.1
prunus_persica Prupe.6G245800_v2.0.a1
pyrus_communis pycom04g10600 pycom12g12720
rosa_chinensis RchiOBHm_Chr3g0468021
rosa_laevigata RLG00000024458
rosa_multiflora Rmu_sc0006706.1_g000009
rosa_roxburghii Rroxscaffold_6G00413170
rosa_rugosa Rorug03G0091500
rosa_samantha Rh3AG140200 Rh3BG161400 Rh3CG161500 Rh3DG162000
rosa_wichuraiana Rw3G013240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 463
Acc36I ACCTGC 1 cut(s) 463
AccB1I GGYRCC 1 cut(s) 429
AciI CCGC 4 cut(s) 19, 149, 311, 394
AclI AACGTT 1 cut(s) 417
AcoI YGGCCR 1 cut(s) 241
AcsI RAATTY 2 cut(s) 335, 410
AfaI GTAC 2 cut(s) 444, 584
AfiI CCNNNNNNNGG 1 cut(s) 325
AgsI TTSAA 6 cut(s) 238, 370, 400, 415, 638, 656
AjnI CCWGG 1 cut(s) 76
AluBI AGCT 3 cut(s) 16, 24, 69
AluI AGCT 3 cut(s) 16, 24, 69
Alw21I GWGCWC 1 cut(s) 26
AlwNI CAGNNNCTG 1 cut(s) 262
AoxI GGCC 1 cut(s) 241
ApeKI GCWGC 4 cut(s) 16, 69, 256, 572
ApoI RAATTY 2 cut(s) 335, 410
ArsI GACNNNNNNTTYG 4 cut(s) 94, 126, 256, 288
AspLEI GCGC 1 cut(s) 478
AsuHPI GGTGA 2 cut(s) 40, 163
AsuII TTCGAA 1 cut(s) 171
BaeGI GKGCMC 1 cut(s) 434
BanI GGYRCC 1 cut(s) 429
BanII GRGCYC 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 645
Bbv12I GWGCWC 1 cut(s) 26
BbvCI CCTCAGC 1 cut(s) 65
BbvI GCAGC 4 cut(s) 3, 56, 268, 559
BccI CCATC 2 cut(s) 374, 641
BceAI ACGGC 2 cut(s) 256, 524
BciT130I CCWGG 1 cut(s) 78
BciVI GTATCC 1 cut(s) 307
BfaI CTAG 1 cut(s) 27
BfuAI ACCTGC 1 cut(s) 463
BfuI GTATCC 1 cut(s) 307
BisI GCNGC 4 cut(s) 17, 70, 257, 573
BlsI GCNGC 4 cut(s) 18, 71, 258, 574
BmcAI AGTACT 1 cut(s) 584
Bme1390I CCNGG 1 cut(s) 78
BmiI GGNNCC 2 cut(s) 431, 623
BmrFI CCNGG 1 cut(s) 78
BpiI GAAGAC 1 cut(s) 645
Bpu10I CCTNAGC 1 cut(s) 65
Bpu14I TTCGAA 1 cut(s) 171
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 1 cut(s) 325
Bse1I ACTGG 1 cut(s) 590
BseBI CCWGG 1 cut(s) 78
BseGI GGATG 2 cut(s) 334, 652
BseLI CCNNNNNNNGG 1 cut(s) 325
BseMII CTCAG 1 cut(s) 79
BseNI ACTGG 1 cut(s) 590
BseRI GAGGAG 1 cut(s) 197
BseSI GKGCMC 1 cut(s) 434
BseXI GCAGC 4 cut(s) 3, 56, 268, 559
BseYI CCCAGC 1 cut(s) 478
Bsh1285I CGRYCG 2 cut(s) 99, 117
BshFI GGCC 1 cut(s) 243
BshNI GGYRCC 1 cut(s) 429
BsiEI CGRYCG 2 cut(s) 99, 117
BsiHKAI GWGCWC 1 cut(s) 26
BslI CCNNNNNNNGG 1 cut(s) 325
BsmI GAATGC 2 cut(s) 179, 510
BsnI GGCC 1 cut(s) 243
Bsp119I TTCGAA 1 cut(s) 171
Bsp1286I GDGCHC 2 cut(s) 26, 434
Bsp143I GATC 3 cut(s) 96, 114, 610
BspACI CCGC 4 cut(s) 19, 149, 311, 394
BspANI GGCC 1 cut(s) 243
BspCNI CTCAG 1 cut(s) 78
BspLI GGNNCC 2 cut(s) 431, 623
BspMI ACCTGC 1 cut(s) 463
BspT104I TTCGAA 1 cut(s) 171
BspT107I GGYRCC 1 cut(s) 429
BsrI ACTGG 1 cut(s) 590
BssMI GATC 3 cut(s) 96, 114, 610
Bst2UI CCWGG 1 cut(s) 78
Bst4CI ACNGT 1 cut(s) 633
Bst6I CTCTTC 1 cut(s) 593
BstBI TTCGAA 1 cut(s) 171
BstC8I GCNNGC 1 cut(s) 290
BstDEI CTNAG 1 cut(s) 65
BstF5I GGATG 2 cut(s) 334, 652
BstHHI GCGC 1 cut(s) 478
BstKTI GATC 3 cut(s) 99, 117, 613
BstMBI GATC 3 cut(s) 96, 114, 610
BstMCI CGRYCG 2 cut(s) 99, 117
BstMWI GCNNNNNNNGC 1 cut(s) 545
BstNI CCWGG 1 cut(s) 78
BstNSI RCATGY 1 cut(s) 512
BstSCI CCNGG 1 cut(s) 76
BstSLI GKGCMC 1 cut(s) 434
BstV1I GCAGC 4 cut(s) 3, 56, 268, 559
BstV2I GAAGAC 1 cut(s) 645
BstXI CCANNNNNNTGG 1 cut(s) 478
BsuI GTATCC 1 cut(s) 307
BsuRI GGCC 1 cut(s) 243
BtgZI GCGATG 1 cut(s) 307
BtsCI GGATG 2 cut(s) 334, 652
BtsI GCAGTG 2 cut(s) 471, 555
BtsIMutI CAGTG 2 cut(s) 471, 555
BveI ACCTGC 1 cut(s) 463
Cac8I GCNNGC 1 cut(s) 290
CaiI CAGNNNCTG 1 cut(s) 262
CfoI GCGC 1 cut(s) 478
CseI GACGC 1 cut(s) 112
Csp6I GTAC 2 cut(s) 443, 583
CspCI CAANNNNNGTGG 2 cut(s) 277, 312
CviAII CATG 2 cut(s) 134, 509
CviJI RGCY 9 cut(s) 16, 24, 69, 243, 259, 539, 567, 617, 670
CviKI_1 RGCY 9 cut(s) 16, 24, 69, 243, 259, 539, 567, 617, 670
CviQI GTAC 2 cut(s) 443, 583
DdeI CTNAG 1 cut(s) 65
DpnI GATC 3 cut(s) 98, 116, 612
DpnII GATC 3 cut(s) 96, 114, 610
EaeI YGGCCR 1 cut(s) 241
Eam1104I CTCTTC 1 cut(s) 593
EarI CTCTTC 1 cut(s) 593
EciI GGCGGA 1 cut(s) 326
Ecl136II GAGCTC 1 cut(s) 24
Eco24I GRGCYC 1 cut(s) 26
Eco53kI GAGCTC 1 cut(s) 24
EcoICRI GAGCTC 1 cut(s) 24
EcoRI GAATTC 1 cut(s) 410
EcoRII CCWGG 1 cut(s) 76
EcoT22I ATGCAT 1 cut(s) 510
EcoT38I GRGCYC 1 cut(s) 26
FaeI CATG 2 cut(s) 137, 512
FatI CATG 2 cut(s) 133, 508
Fnu4HI GCNGC 4 cut(s) 17, 70, 257, 573
FokI GGATG 2 cut(s) 341, 659
FriOI GRGCYC 1 cut(s) 26
Fsp4HI GCNGC 4 cut(s) 17, 70, 257, 573
FspBI CTAG 1 cut(s) 27
GlaI GCGC 1 cut(s) 477
GluI GCNGC 4 cut(s) 17, 70, 257, 573
GsaI CCCAGC 1 cut(s) 482
HaeIII GGCC 1 cut(s) 243
HgaI GACGC 1 cut(s) 112
HhaI GCGC 1 cut(s) 478
Hin1II CATG 2 cut(s) 137, 512
Hin6I GCGC 1 cut(s) 476
HinP1I GCGC 1 cut(s) 476
HinfI GANTC 3 cut(s) 110, 196, 248
HphI GGTGA 2 cut(s) 40, 163
Hpy166II GTNNAC 2 cut(s) 48, 443
Hpy188I TCNGA 5 cut(s) 264, 328, 364, 379, 686
Hpy188III TCNNGA 4 cut(s) 100, 118, 203, 400
Hpy8I GTNNAC 2 cut(s) 48, 443
HpyAV CCTTC 5 cut(s) 61, 154, 205, 369, 493
HpyCH4III ACNGT 1 cut(s) 633
HpyCH4IV ACGT 3 cut(s) 222, 417, 422
HpyCH4V TGCA 4 cut(s) 177, 288, 508, 575
HpyF10VI GCNNNNNNNGC 1 cut(s) 545
HpyF3I CTNAG 1 cut(s) 65
HpySE526I ACGT 3 cut(s) 222, 417, 422
Hsp92II CATG 2 cut(s) 137, 512
HspAI GCGC 1 cut(s) 476
Kzo9I GATC 3 cut(s) 96, 114, 610
LmnI GCTCC 2 cut(s) 13, 21
Lsp1109I GCAGC 4 cut(s) 3, 56, 268, 559
MaeI CTAG 1 cut(s) 27
MaeII ACGT 3 cut(s) 222, 417, 422
MaeIII GTNAC 2 cut(s) 100, 418
MalI GATC 3 cut(s) 98, 116, 612
MboI GATC 3 cut(s) 96, 114, 610
MboII GAAGA 7 cut(s) 191, 229, 500, 607, 610, 650, 668
MhlI GDGCHC 2 cut(s) 26, 434
MluCI AATT 6 cut(s) 40, 137, 232, 335, 350, 410
MlyI GAGTC 1 cut(s) 257
MnlI CCTC 4 cut(s) 74, 175, 270, 301
Mph1103I ATGCAT 1 cut(s) 510
MseI TTAA 2 cut(s) 321, 527
MspA1I CMGCKG 1 cut(s) 69
MspR9I CCNGG 1 cut(s) 78
Mva1269I GAATGC 2 cut(s) 179, 510
MvaI CCWGG 1 cut(s) 78
MwoI GCNNNNNNNGC 1 cut(s) 545
NdeII GATC 3 cut(s) 96, 114, 610
NlaIII CATG 2 cut(s) 137, 512
NlaIV GGNNCC 2 cut(s) 431, 623
NmuCI GTSAC 1 cut(s) 100
NsiI ATGCAT 1 cut(s) 510
NspI RCATGY 1 cut(s) 512
NspV TTCGAA 1 cut(s) 171
PaqCI CACCTGC 1 cut(s) 463
PcsI WCGNNNNNNNCGW 1 cut(s) 110
PctI GAATGC 2 cut(s) 179, 510
PfeI GAWTC 2 cut(s) 110, 196
PfoI TCCNGGA 1 cut(s) 76
PkrI GCNGC 4 cut(s) 18, 71, 258, 574
Ple19I CGATCG 2 cut(s) 99, 117
PleI GAGTC 1 cut(s) 256
PpsI GAGTC 1 cut(s) 256
Psp124BI GAGCTC 1 cut(s) 26
Psp1406I AACGTT 1 cut(s) 417
Psp6I CCWGG 1 cut(s) 76
PspFI CCCAGC 1 cut(s) 478
PspGI CCWGG 1 cut(s) 76
PspN4I GGNNCC 2 cut(s) 431, 623
PstNI CAGNNNCTG 1 cut(s) 262
PvuI CGATCG 2 cut(s) 99, 117
PvuII CAGCTG 1 cut(s) 69
RsaI GTAC 2 cut(s) 444, 584
RsaNI GTAC 2 cut(s) 443, 583
SacI GAGCTC 1 cut(s) 26
SaqAI TTAA 2 cut(s) 321, 527
SatI GCNGC 4 cut(s) 17, 70, 257, 573
Sau3AI GATC 3 cut(s) 96, 114, 610
ScaI AGTACT 1 cut(s) 584
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 1 cut(s) 78
SduI GDGCHC 2 cut(s) 26, 434
SfuI TTCGAA 1 cut(s) 171
Sse9I AATT 6 cut(s) 40, 137, 232, 335, 350, 410
SsiI CCGC 4 cut(s) 19, 149, 311, 394
SspMI CTAG 1 cut(s) 27
SstI GAGCTC 1 cut(s) 26
StyD4I CCNGG 1 cut(s) 76
TaaI ACNGT 1 cut(s) 633
TaiI ACGT 3 cut(s) 225, 420, 425
TaqI TCGA 4 cut(s) 5, 113, 171, 339
TasI AATT 6 cut(s) 40, 137, 232, 335, 350, 410
TatI WGTACW 1 cut(s) 582
TfiI GAWTC 2 cut(s) 110, 196
Tru1I TTAA 2 cut(s) 321, 527
Tru9I TTAA 2 cut(s) 321, 527
TscAI CASTG 2 cut(s) 478, 555
TseFI GTSAC 1 cut(s) 100
TseI GCWGC 4 cut(s) 16, 69, 256, 572
Tsp45I GTSAC 1 cut(s) 100
TspDTI ATGAA 2 cut(s) 150, 675
TspGWI ACGGA 1 cut(s) 25
TspRI CASTG 2 cut(s) 478, 555
XapI RAATTY 2 cut(s) 335, 410
XceI RCATGY 1 cut(s) 512
XcmI CCANNNNNNNNNTGG 1 cut(s) 475
XspI CTAG 1 cut(s) 27
ZrmI AGTACT 1 cut(s) 584
Zsp2I ATGCAT 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.