AT4G00400

glycerol-3-phosphate acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013987
Physical Location & Seq
Reverse (-)
173953 .. 176870
2918 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G00400.1

Sequence Viewer

Length: 1503 bp
ATGTCTCCGGAGAAGAAGAGTCAAAACTTCCCTCCAATAACGGAATGCAGAGACGGAGAGTACGATTCGATAGCCGCCGATCTTGACGGGACTCTGCTTCTCTCAAGAAGCTCCTTCCCTTACTTCATGCTCGTCGCTGTTGAAGCTGGAAGCCTTTTACGTGGACTAATCCTCCTTCTCTCGTTACCATTCGTCATTATCTCTTACCTATTCGTATCCGAATCTCTTGGTATCCAGATCCTCATCTTCATCTCATTCGCTGGTCTCAAAATCCGCGATATCGAACTTGTCTCTCGCGCAGTTCTTCCACGGTTTTACGCGGCGGATGTGAGGAAAGACAGTTTTGAGGTGTTTGATAAGTGTAAGAGAAAAGTGGTAGTGACGGCGAATCCGATTGTGATGGTGGAGGCGTTTGTGAAGGATTATCTTGGAGGTGATAAAGTTTTGGGAACAGAGATTGAAGTTAACCCTAAAACCAATAGAGCCACTGGATTTGTGAAGAAGCCTGGTGTTCTTGTTGGTGATCTTAAGAGGTTAGCCATTTTAAAAGAGTTTGGTAACGAATCACCTGATCTCGGCCTCGGTGATCGAACCTCTGATCATGATTTCATGTCTCTCTGCAAGAAAGGTTACATGGTTCATGCGACCAAGTCAGCCACAACGATTCCAAAAGAACGCTTAAAGAACCGCATAGTCTTCCATGATGGGCGTTTAGCGCAACGTCCAACTCCGTTAAACGCCATTATCACATACCTATGGCTTCCTTTTGGTTTCATCCTCTCCATCATTCGCGTCTACTTCAACCTCCCTTTACCTGAAAGATTTGTCCGTTACACTTACGAGATGCTCGGGATCCACTTAACCATTCGTGGTCATCGTCCTCCACCTCCTTCCCCTGGAACTCTTGGCAACCTCTATGTCCTTAACCACCGTACCGCGCTTGATCCCATCATCGTTGCTATTGCTCTTGGACGTAAGATCTGTTGCGTCACTTACAGTGTCTCTCGTCTCTCCCTTATGCTTTCTCCTATTCCTGCTGTTGCCCTCACCCGTGACCGTGCCACCGATGCTGCCAACATGAGAAAACTTCTCGAGAAAGGCGACTTGGTGATATGTCCGGAAGGCACGACGTGTAGAGAAGAGTATCTACTGAGATTTAGCGCTCTATTCGCAGAGCTAAGCGACCGGATTGTGCCAGTAGCGATGAACTGTAAACAAGGAATGTTCAACGGGACCACAGTTAGGGGTGTGAAGTTTTGGGACCCTTACTTCTTCTTCATGAACCCAAGACCAAGCTATGAAGCCACTTTCTTGGATCGTTTGCCTGAAGAAATGACTGTCAACGGTGGTGGCAAGACTCCTATAGAGGTGGCTAATTACGTCCAGAAAGTTATCGGCGCGGTTTTGGGCTTCGAATGCACCGAACTTACTCGCAAGGATAAATATCTTTTGCTTGGAGGTAATGACGGCAAGGTGGAGTCTATCAACAACACCAAGAAGTGA

Protein Analysis

500

Amino Acids

55.87

Weight (kDa)

9.14

Isoelectric Point (pI)

35.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAD_RAM2_N PF23270 24 - 213 3.1e-63 Glycerol-3-phosphate acyltransferase RAM2-like, HAD-like domain
HAD PF12710 25 - 204 6.5e-20 haloacid dehalogenase-like hydrolase
Acyltransferase PF01553 304 - 402 3.3e-07 Acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013987)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01610 AT4G00400
fragaria_vesca FvH4_7g14440
malus_domestica MD07G1136200.v1.1
prunus_persica Prupe.2G171400_v2.0.a1 Prupe.2G171400_v2.0.a1
pyrus_communis pycom07g13350
rosa_chinensis RchiOBHm_Chr1g0355841
rosa_laevigata RLG00000028126
rosa_multiflora Rmu_sc0002284.1_g000011 Rmu_sc0004705.1_g000024
rosa_roxburghii Rroxscaffold_4G00299980
rosa_rugosa Rorug01G0248100
rosa_samantha Rh1BG230400 Rh1CG244100 Rh1DG258500
rosa_wichuraiana Rw1G023030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 795
AccII CGCG 6 cut(s) 276, 297, 320, 792, 938, 1400
AccIII TCCGGA 2 cut(s) 7, 1117
AciI CCGC 7 cut(s) 75, 274, 320, 323, 688, 936, 1400
AclWI GGATC 5 cut(s) 232, 847, 860, 938, 1323
AcuI CTGAAG 1 cut(s) 1347
AdeI CACNNNGTG 1 cut(s) 1131
AfaI GTAC 2 cut(s) 62, 934
AfeI AGCGCT 1 cut(s) 1162
AfiI CCNNNNNNNGG 3 cut(s) 575, 896, 1242
AflII CTTAAG 1 cut(s) 527
AflIII ACRYGT 1 cut(s) 1130
AgsI TTSAA 4 cut(s) 143, 461, 802, 1228
AjiI CACGTC 1 cut(s) 1131
AjnI CCWGG 2 cut(s) 505, 895
AjuI GAANNNNNNNTTGG 2 cut(s) 1088, 1120
AluBI AGCT 4 cut(s) 111, 146, 1177, 1296
AluI AGCT 4 cut(s) 111, 146, 1177, 1296
Alw26I GTCTC 7 cut(s) 9, 45, 269, 295, 618, 1006, 1013
AlwI GGATC 5 cut(s) 232, 847, 860, 938, 1323
Ama87I CYCGRG 2 cut(s) 848, 1091
Aor13HI TCCGGA 2 cut(s) 7, 1117
Aor51HI AGCGCT 1 cut(s) 1162
AoxI GGCC 1 cut(s) 577
ApeKI GCWGC 1 cut(s) 1070
AspLEI GCGC 5 cut(s) 299, 718, 940, 1163, 1400
AspS9I GGNCC 2 cut(s) 1233, 1261
AsuHPI GGTGA 6 cut(s) 446, 533, 558, 596, 1039, 1120
AsuII TTCGAA 1 cut(s) 1413
AvaI CYCGRG 2 cut(s) 848, 1091
AvaII GGWCC 2 cut(s) 1233, 1261
BaeI ACNNNNGTAYC 2 cut(s) 916, 949
BamHI GGATCC 1 cut(s) 852
BarI GAAGNNNNNNTAC 2 cut(s) 1131, 1163
BbsI GAAGAC 1 cut(s) 688
BbvI GCAGC 1 cut(s) 1057
BccI CCATC 4 cut(s) 394, 698, 791, 956
BceAI ACGGC 2 cut(s) 399, 1483
BciT130I CCWGG 2 cut(s) 507, 897
BciVI GTATCC 2 cut(s) 226, 242
BclI TGATCA 1 cut(s) 598
BcoDI GTCTC 7 cut(s) 9, 45, 269, 295, 618, 1006, 1013
BfmI CTRYAG 1 cut(s) 1362
BfoI RGCGCY 1 cut(s) 1164
BfrI CTTAAG 1 cut(s) 527
BfuI GTATCC 2 cut(s) 226, 242
BglII AGATCT 1 cut(s) 978
BisI GCNGC 3 cut(s) 75, 321, 1071
BlpI GCTNAGC 1 cut(s) 1178
BlsI GCNGC 3 cut(s) 76, 322, 1072
Bme1390I CCNGG 2 cut(s) 507, 897
Bme18I GGWCC 2 cut(s) 1233, 1261
BmeT110I CYCGRG 2 cut(s) 848, 1091
BmgBI CACGTC 1 cut(s) 1131
BmgT120I GGNCC 2 cut(s) 1233, 1261
BmiI GGNNCC 4 cut(s) 854, 1234, 1262, 1263
BmrFI CCNGG 2 cut(s) 507, 897
BmsI GCATC 2 cut(s) 834, 1057
BpiI GAAGAC 1 cut(s) 688
Bpu1102I GCTNAGC 1 cut(s) 1178
Bpu14I TTCGAA 1 cut(s) 1413
BpuEI CTTGAG 1 cut(s) 88
BsaAI YACGTR 1 cut(s) 161
BsaBI GATNNNNATC 2 cut(s) 242, 1443
BsaI GGTCTC 1 cut(s) 269
BsaJI CCNNGG 3 cut(s) 308, 580, 895
BsaWI WCCGGW 3 cut(s) 7, 1117, 1185
BsaXI ACNNNNNCTCC 2 cut(s) 156, 186
Bsc4I CCNNNNNNNGG 3 cut(s) 575, 896, 1242
Bse1I ACTGG 2 cut(s) 493, 1196
Bse8I GATNNNNATC 2 cut(s) 242, 1443
BseAI TCCGGA 2 cut(s) 7, 1117
BseBI CCWGG 2 cut(s) 507, 897
BseDI CCNNGG 3 cut(s) 308, 580, 895
BseGI GGATG 2 cut(s) 331, 774
BseJI GATNNNNATC 2 cut(s) 242, 1443
BseLI CCNNNNNNNGG 3 cut(s) 575, 896, 1242
BseMII CTCAG 1 cut(s) 1142
BseNI ACTGG 2 cut(s) 493, 1196
BseXI GCAGC 1 cut(s) 1057
Bsh1236I CGCG 6 cut(s) 276, 297, 320, 792, 938, 1400
Bsh1285I CGRYCG 1 cut(s) 1186
BshFI GGCC 1 cut(s) 579
BsiEI CGRYCG 1 cut(s) 1186
BsiHKCI CYCGRG 2 cut(s) 848, 1091
BsiSI CCGG 3 cut(s) 8, 1118, 1186
BslFI GGGAC 3 cut(s) 103, 1246, 1274
BslI CCNNNNNNNGG 3 cut(s) 575, 896, 1242
BsmAI GTCTC 7 cut(s) 9, 45, 269, 295, 618, 1006, 1013
BsmBI CGTCTC 2 cut(s) 45, 1013
BsmFI GGGAC 3 cut(s) 103, 1246, 1274
BsmI GAATGC 2 cut(s) 50, 1421
BsnI GGCC 1 cut(s) 579
Bso31I GGTCTC 1 cut(s) 269
BsoBI CYCGRG 2 cut(s) 848, 1091
Bsp119I TTCGAA 1 cut(s) 1413
Bsp13I TCCGGA 2 cut(s) 7, 1117
Bsp1720I GCTNAGC 1 cut(s) 1178
BspACI CCGC 7 cut(s) 75, 274, 320, 323, 688, 936, 1400
BspANI GGCC 1 cut(s) 579
BspCNI CTCAG 1 cut(s) 1143
BspEI TCCGGA 2 cut(s) 7, 1117
BspFNI CGCG 6 cut(s) 276, 297, 320, 792, 938, 1400
BspHI TCATGA 2 cut(s) 601, 1278
BspLI GGNNCC 4 cut(s) 854, 1234, 1262, 1263
BspPI GGATC 5 cut(s) 232, 847, 860, 938, 1323
BspT104I TTCGAA 1 cut(s) 1413
BspTI CTTAAG 1 cut(s) 527
BspTNI GGTCTC 1 cut(s) 269
BsrI ACTGG 2 cut(s) 493, 1196
BssECI CCNNGG 3 cut(s) 308, 580, 895
Bst2UI CCWGG 2 cut(s) 507, 897
Bst4CI ACNGT 9 cut(s) 312, 341, 932, 998, 1058, 1211, 1240, 1339, 1346
Bst6I CTCTTC 2 cut(s) 11, 1134
BstAFI CTTAAG 1 cut(s) 527
BstBAI YACGTR 1 cut(s) 161
BstBI TTCGAA 1 cut(s) 1413
BstDEI CTNAG 2 cut(s) 1151, 1178
BstDSI CCRYGG 1 cut(s) 308
BstF5I GGATG 2 cut(s) 331, 774
BstFNI CGCG 6 cut(s) 276, 297, 320, 792, 938, 1400
BstH2I RGCGCY 1 cut(s) 1164
BstHHI GCGC 5 cut(s) 299, 718, 940, 1163, 1400
BstMAI GTCTC 7 cut(s) 9, 45, 269, 295, 618, 1006, 1013
BstMCI CGRYCG 1 cut(s) 1186
BstMWI GCNNNNNNNGC 5 cut(s) 143, 715, 1067, 1169, 1416
BstNI CCWGG 2 cut(s) 507, 897
BstSCI CCNGG 2 cut(s) 505, 895
BstSFI CTRYAG 1 cut(s) 1362
BstUI CGCG 6 cut(s) 276, 297, 320, 792, 938, 1400
BstV1I GCAGC 1 cut(s) 1057
BstV2I GAAGAC 1 cut(s) 688
BstX2I RGATCY 3 cut(s) 237, 852, 978
BstXI CCANNNNNNTGG 1 cut(s) 1312
BstYI RGATCY 3 cut(s) 237, 852, 978
BsuI GTATCC 2 cut(s) 226, 242
BsuRI GGCC 1 cut(s) 579
BtgI CCRYGG 1 cut(s) 308
BtgZI GCGATG 1 cut(s) 1217
BtrI CACGTC 1 cut(s) 1131
BtsCI GGATG 2 cut(s) 331, 774
BtsIMutI CAGTG 2 cut(s) 486, 1003
CciI TCATGA 2 cut(s) 601, 1278
CfoI GCGC 5 cut(s) 299, 718, 940, 1163, 1400
Cfr13I GGNCC 2 cut(s) 1233, 1261
CseI GACGC 2 cut(s) 781, 976
Csp6I GTAC 2 cut(s) 61, 933
CspCI CAANNNNNGTGG 4 cut(s) 475, 510, 1330, 1365
CviAII CATG 8 cut(s) 127, 602, 610, 634, 641, 701, 1078, 1279
CviQI GTAC 2 cut(s) 61, 933
DdeI CTNAG 2 cut(s) 1151, 1178
DraI TTTAAA 1 cut(s) 546
DraIII CACNNNGTG 1 cut(s) 1131
Eam1104I CTCTTC 2 cut(s) 11, 1134
EarI CTCTTC 2 cut(s) 11, 1134
EciI GGCGGA 1 cut(s) 338
Eco31I GGTCTC 1 cut(s) 269
Eco32I GATATC 1 cut(s) 280
Eco47I GGWCC 2 cut(s) 1233, 1261
Eco47III AGCGCT 1 cut(s) 1162
Eco57I CTGAAG 1 cut(s) 1347
Eco88I CYCGRG 2 cut(s) 848, 1091
EcoO109I RGGNCCY 1 cut(s) 1261
EcoRII CCWGG 2 cut(s) 505, 895
EcoRV GATATC 1 cut(s) 280
Esp3I CGTCTC 2 cut(s) 45, 1013
FaeI CATG 8 cut(s) 130, 605, 613, 637, 644, 704, 1081, 1282
FaqI GGGAC 3 cut(s) 103, 1246, 1274
FatI CATG 8 cut(s) 126, 601, 609, 633, 640, 700, 1077, 1278
FbaI TGATCA 1 cut(s) 598
FblI GTMKAC 1 cut(s) 795
Fnu4HI GCNGC 3 cut(s) 75, 321, 1071
FokI GGATG 2 cut(s) 338, 761
Fsp4HI GCNGC 3 cut(s) 75, 321, 1071
GlaI GCGC 5 cut(s) 298, 717, 939, 1162, 1399
GluI GCNGC 3 cut(s) 75, 321, 1071
HaeII RGCGCY 1 cut(s) 1164
HaeIII GGCC 1 cut(s) 579
HapII CCGG 3 cut(s) 8, 1118, 1186
HgaI GACGC 2 cut(s) 781, 976
HhaI GCGC 5 cut(s) 299, 718, 940, 1163, 1400
Hin1II CATG 8 cut(s) 130, 605, 613, 637, 644, 704, 1081, 1282
Hin6I GCGC 5 cut(s) 297, 716, 938, 1161, 1398
HinP1I GCGC 5 cut(s) 297, 716, 938, 1161, 1398
HincII GTYRAC 2 cut(s) 466, 1342
HindII GTYRAC 2 cut(s) 466, 1342
HinfI GANTC 9 cut(s) 19, 65, 91, 221, 388, 563, 664, 1357, 1478
HpaI GTTAAC 1 cut(s) 466
HpaII CCGG 3 cut(s) 8, 1118, 1186
HphI GGTGA 6 cut(s) 446, 533, 558, 596, 1039, 1120
Hpy166II GTNNAC 5 cut(s) 164, 466, 796, 1214, 1342
Hpy188I TCNGA 3 cut(s) 220, 393, 598
Hpy8I GTNNAC 5 cut(s) 164, 466, 796, 1214, 1342
Hpy99I CGWCG 2 cut(s) 137, 1132
HpyAV CCTTC 5 cut(s) 124, 185, 412, 900, 1115
HpyCH4III ACNGT 9 cut(s) 312, 341, 932, 998, 1058, 1211, 1240, 1339, 1346
HpyCH4IV ACGT 5 cut(s) 160, 721, 973, 1130, 1380
HpyCH4V TGCA 3 cut(s) 48, 621, 1419
HpyF10VI GCNNNNNNNGC 5 cut(s) 143, 715, 1067, 1169, 1416
HpyF3I CTNAG 2 cut(s) 1151, 1178
HpySE526I ACGT 5 cut(s) 160, 721, 973, 1130, 1380
Hsp92II CATG 8 cut(s) 130, 605, 613, 637, 644, 704, 1081, 1282
HspAI GCGC 5 cut(s) 297, 716, 938, 1161, 1398
KflI GGGWCCC 1 cut(s) 1261
Kpn2I TCCGGA 2 cut(s) 7, 1117
Ksp22I TGATCA 1 cut(s) 598
KspAI GTTAAC 1 cut(s) 466
LmnI GCTCC 1 cut(s) 116
Lsp1109I GCAGC 1 cut(s) 1057
LweI GCATC 2 cut(s) 834, 1057
MaeII ACGT 5 cut(s) 160, 721, 973, 1130, 1380
MaeIII GTNAC 7 cut(s) 183, 379, 557, 629, 830, 988, 1052
MflI RGATCY 3 cut(s) 237, 852, 978
MluCI AATT 1 cut(s) 1375
MlyI GAGTC 4 cut(s) 28, 85, 1351, 1487
MmeI TCCRAC 1 cut(s) 749
MroI TCCGGA 2 cut(s) 7, 1117
MseI TTAA 7 cut(s) 465, 528, 545, 680, 734, 860, 924
MslI CAYNNNNRTG 1 cut(s) 754
MspCI CTTAAG 1 cut(s) 527
MspI CCGG 3 cut(s) 8, 1118, 1186
MspR9I CCNGG 2 cut(s) 507, 897
Mva1269I GAATGC 2 cut(s) 50, 1421
MvaI CCWGG 2 cut(s) 507, 897
MvnI CGCG 6 cut(s) 276, 297, 320, 792, 938, 1400
MwoI GCNNNNNNNGC 5 cut(s) 143, 715, 1067, 1169, 1416
NlaIII CATG 8 cut(s) 130, 605, 613, 637, 644, 704, 1081, 1282
NlaIV GGNNCC 4 cut(s) 854, 1234, 1262, 1263
NmeAIII GCCGAG 1 cut(s) 555
NmuCI GTSAC 3 cut(s) 379, 988, 1052
NspV TTCGAA 1 cut(s) 1413
PaeR7I CTCGAG 1 cut(s) 1091
PagI TCATGA 2 cut(s) 601, 1278
PcsI WCGNNNNNNNCGW 5 cut(s) 60, 389, 874, 1098, 1419
PctI GAATGC 2 cut(s) 50, 1421
PfeI GAWTC 5 cut(s) 65, 221, 388, 563, 664
PflFI GACNNNGTC 1 cut(s) 649
PkrI GCNGC 3 cut(s) 76, 322, 1072
PleI GAGTC 4 cut(s) 27, 85, 1351, 1486
PpsI GAGTC 4 cut(s) 27, 85, 1351, 1486
Ppu21I YACGTR 1 cut(s) 161
PpuMI RGGWCCY 1 cut(s) 1261
Psp5II RGGWCCY 1 cut(s) 1261
Psp6I CCWGG 2 cut(s) 505, 895
PspGI CCWGG 2 cut(s) 505, 895
PspN4I GGNNCC 4 cut(s) 854, 1234, 1262, 1263
PspPI GGNCC 2 cut(s) 1233, 1261
PspPPI RGGWCCY 1 cut(s) 1261
PsuI RGATCY 3 cut(s) 237, 852, 978
PsyI GACNNNGTC 1 cut(s) 649
RsaI GTAC 2 cut(s) 62, 934
RsaNI GTAC 2 cut(s) 61, 933
RseI CAYNNNNRTG 1 cut(s) 754
SaqAI TTAA 7 cut(s) 465, 528, 545, 680, 734, 860, 924
SatI GCNGC 3 cut(s) 75, 321, 1071
Sau96I GGNCC 2 cut(s) 1233, 1261
SchI GAGTC 4 cut(s) 28, 85, 1351, 1487
ScrFI CCNGG 2 cut(s) 507, 897
SfaNI GCATC 2 cut(s) 834, 1057
SfcI CTRYAG 1 cut(s) 1362
Sfr274I CTCGAG 1 cut(s) 1091
SfuI TTCGAA 1 cut(s) 1413
SinI GGWCC 2 cut(s) 1233, 1261
SlaI CTCGAG 1 cut(s) 1091
SmiMI CAYNNNNRTG 1 cut(s) 754
SmlI CTYRAG 3 cut(s) 103, 527, 1091
SmoI CTYRAG 3 cut(s) 103, 527, 1091
Sse9I AATT 1 cut(s) 1375
SsiI CCGC 7 cut(s) 75, 274, 320, 323, 688, 936, 1400
StyD4I CCNGG 2 cut(s) 505, 895
TaaI ACNGT 9 cut(s) 312, 341, 932, 998, 1058, 1211, 1240, 1339, 1346
TaiI ACGT 5 cut(s) 163, 724, 976, 1133, 1383
TaqI TCGA 5 cut(s) 68, 282, 589, 1092, 1413
TasI AATT 1 cut(s) 1375
TauI GCSGC 2 cut(s) 77, 323
TfiI GAWTC 5 cut(s) 65, 221, 388, 563, 664
Tru1I TTAA 7 cut(s) 465, 528, 545, 680, 734, 860, 924
Tru9I TTAA 7 cut(s) 465, 528, 545, 680, 734, 860, 924
TscAI CASTG 2 cut(s) 493, 1003
TseFI GTSAC 3 cut(s) 379, 988, 1052
TseI GCWGC 1 cut(s) 1070
Tsp45I GTSAC 3 cut(s) 379, 988, 1052
TspDTI ATGAA 9 cut(s) 115, 238, 598, 629, 763, 1220, 1267, 1295, 1314
TspGWI ACGGA 4 cut(s) 56, 69, 720, 818
TspRI CASTG 2 cut(s) 493, 1003
Tth111I GACNNNGTC 1 cut(s) 649
Vha464I CTTAAG 1 cut(s) 527
VpaK11BI GGWCC 2 cut(s) 1233, 1261
XhoI CTCGAG 1 cut(s) 1091
XmiI GTMKAC 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.