Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0013987

Physical Location & Seq

Genomic Coordinates Reverse (-)

19837868 .. 19840943

Gene Structure 3076 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1136200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1503 bp

ATGTCGTCGCAGAAGCAACGGCGGAGCTTCCCGTCGATCGCGGCCTGCAACGTGGGCGAAACGGCGTCGTACGAGTCGATCGCTGCCGACCTGGACGGGACGCTGCTCATCTCCCGGAGCTCCTTCCCTTACTTCATGCTGGTGGCTATCGAAGCCGGAAGCCTCCTCCGCGGCCTTGTCCTCCTCCTTTCCCTCCCGCTCGTCATCGTCTCCTACTTCTTCTTCTCCGAAGCCGTCGGCATCCAGATCCTCATCTTCATCTCCTTCTCCGGCCTCAAAATCCGCGACATCGAGCTCGCCTCCCGAGCCGTCCTACCCCGGTTTTACGCGGCGGATGTGAGGGCGGAGAGCTTCGAGGTGTTCGACAAATGCAAGAGAAAGGTGGTGGTGACGGCGAATCCGACGGTGATGGTGGAGCCGTTCGTGAAGGACTTTTTGGGTGGGGACAAGGTGCTGGGGACGGAGATCGAAGTGAATCCGAAGACGAAGAAGGCGACCGGGTTCGTGAAGAAGCCCGGGGTTTTGGTGGGAAAATTGAAGCGGTTGGCGGTTATGAAGGAGTTCGGCGACGAAGTGCCGGATCTCGGGATCGGCGATCGGAGCTCCGATCATGACTACATGTCACTTTGCAAGGAGGGTTACATGGTGCCGCCCAACAAATCAGCCCGTCCAATTTCAAAGAACCGTCTCAAAAACCAAATAATTTTCCACGACGGACGCCTAGTGCAGCGCCCGGACCCGCTCAACGCCATCTTAACCTATCTCTGGCTGCCATTTGGCTTCATCCTATGCATTATGCGTGTGTACATCAACCTCCCTCTCCCCGAACGCATCGTCCCCTACACGTACGCAATGCTCGGGATCAAGCTCAAAATCCGCGGCAAGCGTCCCCCGCCGCCCTCCCCCGGCACCCCAGGAAACCTCTACGTCTGCAACCACCGCTCGGCGCTTGACCCGATCATCATCGCCATCGCGCTCGGCCGCAAAGTCTCCTGCGTGTCGTACAGCGTGTCCCGCCTCTCCCGGTTCCTATCTCCAATCCCGACCGTCGCTTTGACTCGCGATCGCGAGGCCGACGCAGCACGACTGTCGTCGCTCCTCCAAAAGGGGGACCTCGTGGTGTGTCCTGAGGGCACCACGTGTCGCGAGCCGTTCCTGCTGCGATTCAGTGCTTTGTTCGCAGAGATGAGCGATAGGATCGTCCCCGTGGCGGTCGACTGCAAGCAGAGCATGTTCTACGGGACCACCGTCCGCGGGGTCAAGTTCTGGGACCCGTACTTCTTTTTCATGAACCCGAGGCCGACGTACGAGGCGACATTTCTGGACCGGTTGCCGGACGACATGTCGTGCAAGGCCGGGAAGTCGTCGATTGAGGTGGCGAATTACGTGCAGAAGGTGTTGGGGGATGTGCTGGGGTTCGAGCGCACCGGATTGACAAGGAAGGATAAATATTTGCTGTTGGGAGGAAATGACGGGAAGGTGGAGTCCATGTACAAGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009987 GO:0010143 GO:0012505 GO:0016020 GO:0016311 GO:0016740 GO:0016746 GO:0016747 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:0090447 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110

Pfam Domains

Protein Families

Acyltransferase HAD

Protein Analysis

501

Amino Acids

55.68

Weight (kDa)

9.41

Isoelectric Point (pI)

44.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAD_RAM2_N	PF23270	27 - 216	1.4e-62	Glycerol-3-phosphate acyltransferase RAM2-like, HAD-like domain
HAD	PF12710	28 - 207	3.5e-21	haloacid dehalogenase-like hydrolase
Acyltransferase	PF01553	304 - 405	6.3e-08	Acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013987)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01610 AT4G00400
fragaria_vesca	FvH4_7g14440
malus_domestica	MD07G1136200.v1.1
prunus_persica	Prupe.2G171400_v2.0.a1 Prupe.2G171400_v2.0.a1
pyrus_communis	pycom07g13350
rosa_chinensis	RchiOBHm_Chr1g0355841
rosa_laevigata	RLG00000028126
rosa_multiflora	Rmu_sc0002284.1_g000011 Rmu_sc0004705.1_g000024
rosa_roxburghii	Rroxscaffold_4G00299980
rosa_rugosa	Rorug01G0248100
rosa_samantha	Rh1BG230400 Rh1CG244100 Rh1DG258500
rosa_wichuraiana	Rw1G023030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	646, 908, 1133
AccBSI	CCGCTC	3 cut(s)	199, 742, 942
AccI	GTMKAC	1 cut(s)	1215
AclWI	GGATC	5 cut(s)	241, 588, 596, 869, 1205
AcoI	YGGCCR	1 cut(s)	979
AcvI	CACGTG	1 cut(s)	1140
AcyI	GRCGYC	2 cut(s)	65, 718
AdeI	CACNNNGTG	1 cut(s)	1140
AfaI	GTAC	7 cut(s)	71, 806, 848, 1004, 1277, 1307, 1493
AfiI	CCNNNNNNNGG	9 cut(s)	584, 765, 905, 943, 1105, 1108, 1210, 1254, 1333
AflIII	ACRYGT	4 cut(s)	618, 843, 1139, 1341
AgeI	ACCGGT	1 cut(s)	1326
AgsI	TTSAA	2 cut(s)	538, 678
AhdI	GACNNNNNGTC	2 cut(s)	619, 1342
AjnI	CCWGG	2 cut(s)	90, 913
AjuI	GAANNNNNNNTTGG	2 cut(s)	419, 451
AloI	GAACNNNNNNTCC	2 cut(s)	819, 851
AluBI	AGCT	6 cut(s)	27, 120, 295, 351, 603, 868
AluI	AGCT	6 cut(s)	27, 120, 295, 351, 603, 868
Alw21I	GWGCWC	3 cut(s)	122, 297, 605
Alw26I	GTCTC	3 cut(s)	214, 692, 994
AlwI	GGATC	5 cut(s)	241, 588, 596, 869, 1205
Ama87I	CYCGRG	5 cut(s)	303, 515, 584, 857, 1294
AoxI	GGCC	7 cut(s)	42, 172, 271, 979, 1071, 1298, 1353
ApeKI	GCWGC	6 cut(s)	83, 103, 727, 769, 1079, 1159
ArsI	GACNNNNNNTTYG	4 cut(s)	819, 851, 1036, 1068
AsiGI	ACCGGT	1 cut(s)	1326
AsiSI	GCGATCGC	1 cut(s)	1066
Asp700I	GAANNNNTTC	1 cut(s)	560
AspLEI	GCGC	4 cut(s)	732, 949, 976, 1425
AspS9I	GGNCC	5 cut(s)	736, 1111, 1242, 1270, 1324
AsuC2I	CCSGG	9 cut(s)	115, 319, 499, 516, 517, 734, 906, 1024, 1357
AsuHPI	GGTGA	2 cut(s)	400, 418
AvaI	CYCGRG	5 cut(s)	303, 515, 584, 857, 1294
AvaII	GGWCC	5 cut(s)	736, 1111, 1242, 1270, 1324
AxyI	CCTNAGG	1 cut(s)	1128
BaeGI	GKGCMC	1 cut(s)	1136
BanI	GGYRCC	3 cut(s)	646, 908, 1133
BanII	GRGCYC	3 cut(s)	122, 297, 605
BauI	CACGAG	1 cut(s)	1115
BbrPI	CACGTG	1 cut(s)	1140
BbsI	GAAGAC	1 cut(s)	488
Bbv12I	GWGCWC	3 cut(s)	122, 297, 605
BbvI	GCAGC	6 cut(s)	70, 90, 739, 756, 1091, 1146
BccI	CCATC	3 cut(s)	403, 758, 977
BceAI	ACGGC	7 cut(s)	35, 78, 218, 293, 403, 408, 1135
BcgI	CGANNNNNNTGC	4 cut(s)	1071, 1105, 1369, 1403
BciT130I	CCWGG	2 cut(s)	92, 915
BcnI	CCSGG	9 cut(s)	115, 319, 499, 516, 517, 734, 906, 1024, 1357
BcoDI	GTCTC	3 cut(s)	214, 692, 994
BfaI	CTAG	1 cut(s)	722
BfoI	RGCGCY	2 cut(s)	733, 950
Bme18I	GGWCC	5 cut(s)	736, 1111, 1242, 1270, 1324
BmeRI	GACNNNNNGTC	2 cut(s)	619, 1342
BmeT110I	CYCGRG	5 cut(s)	303, 515, 584, 857, 1294
BmgT120I	GGNCC	5 cut(s)	736, 1111, 1242, 1270, 1324
BmsI	GCATC	2 cut(s)	249, 840
BoxI	GACNNNNGTC	2 cut(s)	1090, 1247
BpiI	GAAGAC	1 cut(s)	488
BplI	GAGNNNNNCTC	2 cut(s)	284, 316
BpuMI	CCSGG	9 cut(s)	115, 319, 499, 516, 517, 734, 906, 1024, 1357
BsaAI	YACGTR	3 cut(s)	846, 1140, 1387
BsaBI	GATNNNNATC	2 cut(s)	251, 962
BsaHI	GRCGYC	2 cut(s)	65, 718
BsaWI	WCCGGW	2 cut(s)	1326, 1427
BsaXI	ACNNNNNCTCC	1 cut(s)	1475
Bsc4I	CCNNNNNNNGG	9 cut(s)	584, 765, 905, 943, 1105, 1108, 1210, 1254, 1333
Bse118I	RCCGGY	1 cut(s)	1326
Bse21I	CCTNAGG	1 cut(s)	1128
Bse3DI	GCAATG	1 cut(s)	858
Bse8I	GATNNNNATC	2 cut(s)	251, 962
BseBI	CCWGG	2 cut(s)	92, 915
BseGI	GGATG	4 cut(s)	240, 340, 783, 1411
BseJI	GATNNNNATC	2 cut(s)	251, 962
BseLI	CCNNNNNNNGG	9 cut(s)	584, 765, 905, 943, 1105, 1108, 1210, 1254, 1333
BseMI	GCAATG	1 cut(s)	858
BseMII	CTCAG	1 cut(s)	1119
BseRI	GAGGAG	3 cut(s)	155, 173, 1088
BseSI	GKGCMC	1 cut(s)	1136
BseX3I	CGGCCG	1 cut(s)	979
BseXI	GCAGC	6 cut(s)	70, 90, 739, 756, 1091, 1146
BseYI	CCCAGC	2 cut(s)	454, 1411
BsgI	GTGCAG	2 cut(s)	746, 1409
Bsh1285I	CGRYCG	8 cut(s)	39, 81, 498, 598, 982, 1047, 1066, 1215
BshFI	GGCC	7 cut(s)	44, 174, 273, 981, 1073, 1300, 1355
BshNI	GGYRCC	3 cut(s)	646, 908, 1133
BshTI	ACCGGT	1 cut(s)	1326
BsiEI	CGRYCG	8 cut(s)	39, 81, 498, 598, 982, 1047, 1066, 1215
BsiHKAI	GWGCWC	3 cut(s)	122, 297, 605
BsiHKCI	CYCGRG	5 cut(s)	303, 515, 584, 857, 1294
BsiWI	CGTACG	3 cut(s)	69, 846, 1305
BslI	CCNNNNNNNGG	9 cut(s)	584, 765, 905, 943, 1105, 1108, 1210, 1254, 1333
BsmAI	GTCTC	3 cut(s)	214, 692, 994
BsmBI	CGTCTC	2 cut(s)	214, 692
BsnI	GGCC	7 cut(s)	44, 174, 273, 981, 1073, 1300, 1355
BsoBI	CYCGRG	5 cut(s)	303, 515, 584, 857, 1294
Bsp1286I	GDGCHC	4 cut(s)	122, 297, 605, 1136
Bsp1407I	TGTACA	2 cut(s)	804, 1491
Bsp68I	TCGCGA	3 cut(s)	1062, 1068, 1146
BspANI	GGCC	7 cut(s)	44, 174, 273, 981, 1073, 1300, 1355
BspCNI	CTCAG	1 cut(s)	1120
BspHI	TCATGA	2 cut(s)	610, 1287
BspPI	GGATC	5 cut(s)	241, 588, 596, 869, 1205
BspT107I	GGYRCC	3 cut(s)	646, 908, 1133
BsrBI	CCGCTC	3 cut(s)	199, 742, 942
BsrDI	GCAATG	1 cut(s)	858
BsrFI	RCCGGY	1 cut(s)	1326
BsrGI	TGTACA	2 cut(s)	804, 1491
BssAI	RCCGGY	1 cut(s)	1326
BssNI	GRCGYC	2 cut(s)	65, 718
BssSI	CACGAG	1 cut(s)	1115
Bst2BI	CACGAG	1 cut(s)	1115
Bst2UI	CCWGG	2 cut(s)	92, 915
Bst4CI	ACNGT	5 cut(s)	406, 686, 1048, 1089, 1249
BstACI	GRCGYC	2 cut(s)	65, 718
BstAUI	TGTACA	2 cut(s)	804, 1491
BstBAI	YACGTR	3 cut(s)	846, 1140, 1387
BstC8I	GCNNGC	5 cut(s)	46, 297, 884, 1148, 1223
BstDEI	CTNAG	1 cut(s)	1128
BstDSI	CCRYGG	4 cut(s)	169, 877, 1206, 1252
BstENI	CCTNNNNNAGG	1 cut(s)	1103
BstF5I	GGATG	4 cut(s)	240, 340, 783, 1411
BstH2I	RGCGCY	2 cut(s)	733, 950
BstHHI	GCGC	4 cut(s)	732, 949, 976, 1425
BstMAI	GTCTC	3 cut(s)	214, 692, 994
BstMCI	CGRYCG	8 cut(s)	39, 81, 498, 598, 982, 1047, 1066, 1215
BstNI	CCWGG	2 cut(s)	92, 915
BstNSI	RCATGY	3 cut(s)	622, 1234, 1345
BstPAI	GACNNNNGTC	2 cut(s)	1090, 1247
BstSLI	GKGCMC	1 cut(s)	1136
BstV1I	GCAGC	6 cut(s)	70, 90, 739, 756, 1091, 1146
BstV2I	GAAGAC	1 cut(s)	488
BstX2I	RGATCY	2 cut(s)	246, 580
BstYI	RGATCY	2 cut(s)	246, 580
BstZI	CGGCCG	1 cut(s)	979
Bsu36I	CCTNAGG	1 cut(s)	1128
BsuRI	GGCC	7 cut(s)	44, 174, 273, 981, 1073, 1300, 1355
BtgI	CCRYGG	4 cut(s)	169, 877, 1206, 1252
BtgZI	GCGATG	2 cut(s)	949, 955
BtsCI	GGATG	4 cut(s)	240, 340, 783, 1411
BtsIMutI	CAGTG	1 cut(s)	1174
BtuMI	TCGCGA	3 cut(s)	1062, 1068, 1146
Cac8I	GCNNGC	5 cut(s)	46, 297, 884, 1148, 1223
CciI	TCATGA	2 cut(s)	610, 1287
CfoI	GCGC	4 cut(s)	732, 949, 976, 1425
Cfr10I	RCCGGY	1 cut(s)	1326
Cfr13I	GGNCC	5 cut(s)	736, 1111, 1242, 1270, 1324
Cfr42I	CCGCGG	3 cut(s)	172, 880, 1255
Cfr9I	CCCGGG	1 cut(s)	515
CseI	GACGC	5 cut(s)	54, 109, 726, 875, 1085
Csp6I	GTAC	7 cut(s)	70, 805, 847, 1003, 1276, 1306, 1492
CspAI	ACCGGT	1 cut(s)	1326
CviAII	CATG	8 cut(s)	136, 611, 619, 643, 1231, 1288, 1342, 1489
CviQI	GTAC	7 cut(s)	70, 805, 847, 1003, 1276, 1306, 1492
DdeI	CTNAG	1 cut(s)	1128
DraIII	CACNNNGTG	1 cut(s)	1140
DriI	GACNNNNNGTC	2 cut(s)	619, 1342
EaeI	YGGCCR	1 cut(s)	979
EagI	CGGCCG	1 cut(s)	979
Eam1105I	GACNNNNNGTC	2 cut(s)	619, 1342
EciI	GGCGGA	3 cut(s)	37, 347, 359
Ecl136II	GAGCTC	3 cut(s)	120, 295, 603
EclXI	CGGCCG	1 cut(s)	979
Eco24I	GRGCYC	3 cut(s)	122, 297, 605
Eco47I	GGWCC	5 cut(s)	736, 1111, 1242, 1270, 1324
Eco52I	CGGCCG	1 cut(s)	979
Eco53kI	GAGCTC	3 cut(s)	120, 295, 603
Eco72I	CACGTG	1 cut(s)	1140
Eco81I	CCTNAGG	1 cut(s)	1128
Eco88I	CYCGRG	5 cut(s)	303, 515, 584, 857, 1294
EcoICRI	GAGCTC	3 cut(s)	120, 295, 603
EcoNI	CCTNNNNNAGG	1 cut(s)	1103
EcoO109I	RGGNCCY	2 cut(s)	1111, 1270
EcoRII	CCWGG	2 cut(s)	90, 913
EcoT22I	ATGCAT	1 cut(s)	794
EcoT38I	GRGCYC	3 cut(s)	122, 297, 605
Esp3I	CGTCTC	2 cut(s)	214, 692
FaeI	CATG	8 cut(s)	139, 614, 622, 646, 1234, 1291, 1345, 1492
FatI	CATG	8 cut(s)	135, 610, 618, 642, 1230, 1287, 1341, 1488
FauI	CCCGC	5 cut(s)	204, 747, 900, 1022, 1247
FblI	GTMKAC	1 cut(s)	1215
FokI	GGATG	4 cut(s)	227, 347, 770, 1418
FriOI	GRGCYC	3 cut(s)	122, 297, 605
FspBI	CTAG	1 cut(s)	722
GlaI	GCGC	4 cut(s)	731, 948, 975, 1424
GsaI	CCCAGC	2 cut(s)	458, 1415
HaeII	RGCGCY	2 cut(s)	733, 950
HaeIII	GGCC	7 cut(s)	44, 174, 273, 981, 1073, 1300, 1355
HgaI	GACGC	5 cut(s)	54, 109, 726, 875, 1085
HhaI	GCGC	4 cut(s)	732, 949, 976, 1425
Hin1I	GRCGYC	2 cut(s)	65, 718
Hin1II	CATG	8 cut(s)	139, 614, 622, 646, 1234, 1291, 1345, 1492
Hin6I	GCGC	4 cut(s)	730, 947, 974, 1423
HinP1I	GCGC	4 cut(s)	730, 947, 974, 1423
HincII	GTYRAC	1 cut(s)	1216
HindII	GTYRAC	1 cut(s)	1216
HinfI	GANTC	6 cut(s)	74, 397, 475, 1057, 1164, 1484
HphI	GGTGA	2 cut(s)	400, 418
Hpy166II	GTNNAC	2 cut(s)	805, 1216
Hpy188I	TCNGA	5 cut(s)	229, 402, 480, 600, 607
Hpy8I	GTNNAC	2 cut(s)	805, 1216
HpyAV	CCTTC	8 cut(s)	133, 274, 421, 484, 550, 1387, 1435, 1471
HpyCH4III	ACNGT	5 cut(s)	406, 686, 1048, 1089, 1249
HpyCH4IV	ACGT	6 cut(s)	51, 845, 927, 1139, 1304, 1386
HpyCH4V	TGCA	9 cut(s)	48, 372, 630, 727, 792, 933, 1221, 1350, 1390
HpyF3I	CTNAG	1 cut(s)	1128
HpySE526I	ACGT	6 cut(s)	51, 845, 927, 1139, 1304, 1386
Hsp92I	GRCGYC	2 cut(s)	65, 718
Hsp92II	CATG	8 cut(s)	139, 614, 622, 646, 1234, 1291, 1345, 1492
HspAI	GCGC	4 cut(s)	730, 947, 974, 1423
KflI	GGGWCCC	1 cut(s)	1270
KspI	CCGCGG	3 cut(s)	172, 880, 1255
LmnI	GCTCC	7 cut(s)	24, 117, 125, 415, 600, 608, 1101
Lsp1109I	GCAGC	6 cut(s)	70, 90, 739, 756, 1091, 1146
LweI	GCATC	2 cut(s)	249, 840
MaeI	CTAG	1 cut(s)	722
MaeII	ACGT	6 cut(s)	51, 845, 927, 1139, 1304, 1386
MaeIII	GTNAC	3 cut(s)	388, 621, 638
MbiI	CCGCTC	3 cut(s)	199, 742, 942
MboII	GAAGA	6 cut(s)	211, 214, 247, 493, 499, 520
MflI	RGATCY	2 cut(s)	246, 580
MhlI	GDGCHC	4 cut(s)	122, 297, 605, 1136
MluCI	AATT	4 cut(s)	533, 672, 702, 1381
MlyI	GAGTC	3 cut(s)	83, 1051, 1493
MmeI	TCCRAC	1 cut(s)	425
Mph1103I	ATGCAT	1 cut(s)	794
MroXI	GAANNNNTTC	1 cut(s)	560
MseI	TTAA	1 cut(s)	755
MslI	CAYNNNNRTG	1 cut(s)	140
MspA1I	CMGCKG	3 cut(s)	171, 879, 1254
MvaI	CCWGG	2 cut(s)	92, 915
NciI	CCSGG	9 cut(s)	115, 319, 499, 516, 517, 734, 906, 1024, 1357
NlaIII	CATG	8 cut(s)	139, 614, 622, 646, 1234, 1291, 1345, 1492
NmeAIII	GCCGAG	2 cut(s)	923, 957
NmuCI	GTSAC	2 cut(s)	388, 621
NruI	TCGCGA	3 cut(s)	1062, 1068, 1146
NsiI	ATGCAT	1 cut(s)	794
NspI	RCATGY	3 cut(s)	622, 1234, 1345
PagI	TCATGA	2 cut(s)	610, 1287
PciI	ACATGT	2 cut(s)	618, 1341
PcsI	WCGNNNNNNNCGW	7 cut(s)	74, 360, 398, 491, 591, 1245, 1310
PdmI	GAANNNNTTC	1 cut(s)	560
PfeI	GAWTC	3 cut(s)	397, 475, 1164
Pfl23II	CGTACG	3 cut(s)	69, 846, 1305
PfoI	TCCNGGA	1 cut(s)	113
PinAI	ACCGGT	1 cut(s)	1326
Ple19I	CGATCG	4 cut(s)	39, 81, 598, 1066
PleI	GAGTC	3 cut(s)	82, 1051, 1492
PmaCI	CACGTG	1 cut(s)	1140
PmlI	CACGTG	1 cut(s)	1140
PpsI	GAGTC	3 cut(s)	82, 1051, 1492
Ppu21I	YACGTR	3 cut(s)	846, 1140, 1387
PpuMI	RGGWCCY	2 cut(s)	1111, 1270
PscI	ACATGT	2 cut(s)	618, 1341
PshAI	GACNNNNGTC	2 cut(s)	1090, 1247
Psp124BI	GAGCTC	3 cut(s)	122, 297, 605
Psp5II	RGGWCCY	2 cut(s)	1111, 1270
Psp6I	CCWGG	2 cut(s)	90, 913
PspCI	CACGTG	1 cut(s)	1140
PspFI	CCCAGC	2 cut(s)	454, 1411
PspGI	CCWGG	2 cut(s)	90, 913
PspLI	CGTACG	3 cut(s)	69, 846, 1305
PspPI	GGNCC	5 cut(s)	736, 1111, 1242, 1270, 1324
PspPPI	RGGWCCY	2 cut(s)	1111, 1270
PsuI	RGATCY	2 cut(s)	246, 580
PvuI	CGATCG	4 cut(s)	39, 81, 598, 1066
RgaI	GCGATCGC	1 cut(s)	1066
RruI	TCGCGA	3 cut(s)	1062, 1068, 1146
RsaI	GTAC	7 cut(s)	71, 806, 848, 1004, 1277, 1307, 1493
RsaNI	GTAC	7 cut(s)	70, 805, 847, 1003, 1276, 1306, 1492
RseI	CAYNNNNRTG	1 cut(s)	140
SacI	GAGCTC	3 cut(s)	122, 297, 605
SacII	CCGCGG	3 cut(s)	172, 880, 1255
SalI	GTCGAC	1 cut(s)	1214
SaqAI	TTAA	1 cut(s)	755
Sau96I	GGNCC	5 cut(s)	736, 1111, 1242, 1270, 1324
SchI	GAGTC	3 cut(s)	83, 1051, 1493
SduI	GDGCHC	4 cut(s)	122, 297, 605, 1136
SfaAI	GCGATCGC	1 cut(s)	1066
SfaNI	GCATC	2 cut(s)	249, 840
Sfr303I	CCGCGG	3 cut(s)	172, 880, 1255
SgfI	GCGATCGC	1 cut(s)	1066
SgrBI	CCGCGG	3 cut(s)	172, 880, 1255
SinI	GGWCC	5 cut(s)	736, 1111, 1242, 1270, 1324
SmaI	CCCGGG	1 cut(s)	517
SmiMI	CAYNNNNRTG	1 cut(s)	140
Sse9I	AATT	4 cut(s)	533, 672, 702, 1381
SspI	AATATT	1 cut(s)	1451
SspMI	CTAG	1 cut(s)	722
SstI	GAGCTC	3 cut(s)	122, 297, 605
TaaI	ACNGT	5 cut(s)	406, 686, 1048, 1089, 1249
TaiI	ACGT	6 cut(s)	54, 848, 930, 1142, 1307, 1389
TasI	AATT	4 cut(s)	533, 672, 702, 1381
TatI	WGTACW	2 cut(s)	804, 1491
TauI	GCSGC	7 cut(s)	44, 174, 332, 652, 882, 898, 984
TfiI	GAWTC	3 cut(s)	397, 475, 1164
Tru1I	TTAA	1 cut(s)	755
Tru9I	TTAA	1 cut(s)	755
TscAI	CASTG	1 cut(s)	1174
TseFI	GTSAC	2 cut(s)	388, 621
TseI	GCWGC	6 cut(s)	83, 103, 727, 769, 1079, 1159
Tsp45I	GTSAC	2 cut(s)	388, 621
TspDTI	ATGAA	6 cut(s)	124, 247, 569, 772, 1276, 1304
TspGWI	ACGGA	2 cut(s)	476, 729
TspMI	CCCGGG	1 cut(s)	515
TspRI	CASTG	1 cut(s)	1174
VpaK11BI	GGWCC	5 cut(s)	736, 1111, 1242, 1270, 1324
XagI	CCTNNNNNAGG	1 cut(s)	1103
XceI	RCATGY	3 cut(s)	622, 1234, 1345
XmaI	CCCGGG	1 cut(s)	515
XmiI	GTMKAC	1 cut(s)	1215
XmnI	GAANNNNTTC	1 cut(s)	560
XspI	CTAG	1 cut(s)	722
Zsp2I	ATGCAT	1 cut(s)	794

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.