AT4G04960

Legume lectin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0011839
Physical Location & Seq
Forward (+)
2532906 .. 2535386
2481 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G04960.1

Sequence Viewer

Length: 2061 bp
ATGAAAGCTCTTCTCTTTCTTCTTACCCTTTTCCTCATCCTCCCAAACCCAATCTCAGCCATTGATTTCATCTTCAACGGCTTCAATGACTCATCATCTAATGTCTCCCTCTTCGGGATCGCAACAATCGAATCAAAAATCCTAACTTTAACAAACCAAACATCGTTTGCAACTGGTCGTGCTCTATACAACAGAACCATCCGTACAAAAGATCCAATCACATCCTCTGTTTTACCTTTCTCTACTTCTTTCATCTTCACCATGGCTCCTTACAAGAACACACTTCCTGGTCACGGAATCGTCTTCCTCTTTGCACCATCCACTGGAATCAACGGTTCTAGCTCTGCTCAACACTTAGGTCTCTTCAATTTGACTAACAATGGTAACCCTAGTAATCACATCTTTGGAGTTGAGTTTGATGTCTTTGCTAATCAAGAATTTAGTGACATCGATGCTAATCATGTCGGAATTGATGTTAATTCACTTCATTCAGTATATTCTAACACTTCTGGTTATTGGTCTGATGATGGAGTGGTGTTTAAGCCATTGAAGCTTAACGATGGAAGAAACTATCAAGTTTGGATTGATTATAGAGATTTTGTAGTTAATGTTACTATGCAAGTCGCTGGTAAGATTCGACCTAAGATTCCTTTGCTTAGTACGTCTTTGAATCTCTCTGATGTTGTGGAAGATGAGATGTTTGTTGGATTCACTGCTGCTACAGGGAGATTGGTTCAGAGTCATAAGATCTTAGCTTGGAGTTTTAGTAATTCGAATTTCTCGTTGAGTAATAGTTTGATCACTACTGGTTTACCTTCGTTTGTTTTACCTAAGGACTCGATTGTTAAAGCGAAATGGTTTGTTTTTGTGTTGGTGTTGATCTGTTTCTTGGTTGTTGCTTTGGTTGGTTTGGTGTTGTTTGCTGTGGTGAGAAAGAGATTAGAGAGGGCAAGGAAGAGAGCGTTGATGGAGGATTGGGAGATGGAGTATTGGCCTCATCGGATTCCGTATGAGGAGATTGAATCCGGCACTAAGGGGTTTGATGAGAAGAATGTGATTGGGATTGGCGGGAATGGGAAAGTTTATAAAGGTTTGTTACAAGGAGGGGTTGTGGAAGTTGCGGTTAAGCGGATTTCGCAGGAGAGTAGCGACGGGATGAGAGAGTTTGTGGCTGAGATATCGAGTCTTGGGCGGTTAAAGCACCGGAATTTAGTTTCTTTAAGAGGATGGTGCAAGAAGGAGGTTGGTAGTTTCATGTTGGTTTATGATTACATGGAGAATGGAAGTTTAGATCGATGGATATTCGAAAACGATGAGAAGATTACTACCCTTAGTTGCGAAGAGAGAATTCGTATTTTGAAAGGTGTAGCTTCAGGAATATTGTATTTGCATGAAGGATGGGAGTCTAAGGTTTTACATAGAGATATTAAAGCTAGTAATGTTTTACTTGACCGAGATATGATACCGAGGCTTAGTGATTTTGGGTTAGCTCGGGTTCATGGACACGAGCAACCGGTTAGGACCACGAGGGTGGTTGGTACAGCTGGCTATTTGGCTCCTGAAGTGGTTAAGACGGGGCGTGCATCGACACAGACCGATGTTTTTGCTTACGGGATATTGGTTCTTGAAGTAATGTGTGGGAGAAGACCTATTGAAGAAGGGAAGAAACCGTTGATGGATTGGGTTTGGGGATTAATGGAAAGAGGTGAGATTCTAAATGGGCTTGATCCACAGATGATGATGACACAAGGCGTAACAGAAGTCATTGATGAAGCTGAAAGAGTTTTGCAGCTTGGATTGCTTTGTGCACATCCTGATCCGGCCAAAAGACCGTCGATGAGACAAGTGGTTCAAGTGTTTGAAGGTGATAAGGCTGAGATCTTTGAAGCAGAAAGTAGTGAAGATGTCGAGTCATGGATGTTGATGAAGATGGGATCTCGTGGAAGCTCGCGAGAGTTTTGGTATGGAAGCTCATCACATCCGACGATAGAGCAGATTAGACTTCAGTCTTTGTCTGTGTCTCTTTCGTCTTGGAACAGCTCAATTTTAGAAGGGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lectin_legB Pkinase Pkinase_Tyr

Protein Analysis

686

Amino Acids

76.69

Weight (kDa)

6.21

Isoelectric Point (pI)

31.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 21 - 260 5.7e-65 Legume lectin domain
Lectin_L-type_dom PF18483 32 - 250 2.8e-08 Legume lectin beta-barrel domain
Pkinase PF00069 348 - 618 6.6e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 350 - 619 1.6e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011839)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G04960 AT4G04960
fragaria_vesca FvH4_3g09090
malus_domestica MD05G1338700.v1.1 MD10G1293700.v1.1
prunus_persica Prupe.4G048100_v2.0.a1
pyrus_communis pycom05g29000 pycom10g24560
rosa_chinensis RchiOBHm_Chr5g0008551
rosa_laevigata RLG00000031579 RLG00000031582
rosa_multiflora Rmu_sc0001665.1_g000016
rosa_roxburghii Rroxscaffold_1G00068000
rosa_rugosa Rorug04G0436600
rosa_samantha Rh5AG065400 Rh5BG062800 Rh5CG073000 Rh5DG063300
rosa_wichuraiana Rw5G005550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1086
AccB7I CCANNNNNTGG 1 cut(s) 323
AccII CGCG 1 cut(s) 1951
AciI CCGC 4 cut(s) 1068, 1121, 1129, 1192
AclWI GGATC 5 cut(s) 125, 206, 1721, 1811, 1942
AcoI YGGCCR 1 cut(s) 1821
AcsI RAATTY 4 cut(s) 437, 775, 1207, 1347
AcuI CTGAAG 3 cut(s) 1356, 1581, 1988
AfaI GTAC 3 cut(s) 205, 661, 1540
AfiI CCNNNNNNNGG 3 cut(s) 114, 293, 323
AgeI ACCGGT 1 cut(s) 1513
AjnI CCWGG 1 cut(s) 286
AleI CACNNNNGTG 1 cut(s) 1529
Alw21I GWGCWC 2 cut(s) 184, 1810
Alw26I GTCTC 4 cut(s) 109, 365, 1834, 2025
Alw44I GTGCAC 1 cut(s) 1806
AlwI GGATC 5 cut(s) 125, 206, 1721, 1811, 1942
Ama87I CYCGRG 1 cut(s) 1491
AoxI GGCC 2 cut(s) 992, 1821
ApaLI GTGCAC 1 cut(s) 1806
ApeKI GCWGC 2 cut(s) 716, 1789
ApoI RAATTY 4 cut(s) 437, 775, 1207, 1347
AseI ATTAAT 1 cut(s) 1694
AsiGI ACCGGT 1 cut(s) 1513
AspS9I GGNCC 1 cut(s) 1521
AsuHPI GGTGA 4 cut(s) 250, 940, 1718, 1877
AsuII TTCGAA 2 cut(s) 773, 1305
AvaI CYCGRG 1 cut(s) 1491
AvaII GGWCC 1 cut(s) 1521
AxyI CCTNAGG 1 cut(s) 831
BaeGI GKGCMC 1 cut(s) 1810
BauI CACGAG 3 cut(s) 1505, 1525, 1938
BbsI GAAGAC 2 cut(s) 295, 1651
Bbv12I GWGCWC 2 cut(s) 184, 1810
BbvI GCAGC 2 cut(s) 703, 1801
BceAI ACGGC 1 cut(s) 94
BcgI CGANNNNNNTGC 2 cut(s) 1586, 1620
BciT130I CCWGG 1 cut(s) 288
BclI TGATCA 1 cut(s) 798
BcoDI GTCTC 4 cut(s) 109, 365, 1834, 2025
BfaI CTAG 3 cut(s) 339, 390, 1434
BfmI CTRYAG 1 cut(s) 720
BglII AGATCT 2 cut(s) 747, 1878
BisI GCNGC 2 cut(s) 717, 1790
BlsI GCNGC 2 cut(s) 718, 1791
Bme1390I CCNGG 1 cut(s) 288
Bme18I GGWCC 1 cut(s) 1521
BmeT110I CYCGRG 1 cut(s) 1491
BmgT120I GGNCC 1 cut(s) 1521
BmiI GGNNCC 2 cut(s) 267, 1557
BmrFI CCNGG 1 cut(s) 288
BmsI GCATC 2 cut(s) 442, 1592
BoxI GACNNNNGTC 1 cut(s) 2005
BpiI GAAGAC 2 cut(s) 295, 1651
Bpu14I TTCGAA 2 cut(s) 773, 1305
Bsa29I ATCGAT 2 cut(s) 450, 1294
BsaBI GATNNNNATC 1 cut(s) 456
BsaI GGTCTC 1 cut(s) 365
BsaJI CCNNGG 2 cut(s) 261, 1466
BsaWI WCCGGW 2 cut(s) 1203, 1513
BsaXI ACNNNNNCTCC 8 cut(s) 250, 280, 751, 781, 971, 1001, 1095, 1125
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 293, 323
Bse118I RCCGGY 1 cut(s) 1513
Bse1I ACTGG 3 cut(s) 178, 328, 811
Bse21I CCTNAGG 1 cut(s) 831
Bse8I GATNNNNATC 1 cut(s) 456
BseBI CCWGG 1 cut(s) 288
BseCI ATCGAT 2 cut(s) 450, 1294
BseDI CCNNGG 2 cut(s) 261, 1466
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 3 cut(s) 114, 293, 323
BseMII CTCAG 3 cut(s) 69, 1164, 1866
BseNI ACTGG 3 cut(s) 178, 328, 811
BseRI GAGGAG 1 cut(s) 1028
BseSI GKGCMC 1 cut(s) 1810
BseXI GCAGC 2 cut(s) 703, 1801
Bsh1236I CGCG 1 cut(s) 1951
BshFI GGCC 2 cut(s) 994, 1823
BshTI ACCGGT 1 cut(s) 1513
BshVI ATCGAT 2 cut(s) 450, 1294
BsiHKAI GWGCWC 2 cut(s) 184, 1810
BsiHKCI CYCGRG 1 cut(s) 1491
BsiSI CCGG 4 cut(s) 1026, 1204, 1514, 1820
BslI CCNNNNNNNGG 3 cut(s) 114, 293, 323
BsmAI GTCTC 4 cut(s) 109, 365, 1834, 2025
BsnI GGCC 2 cut(s) 994, 1823
Bso31I GGTCTC 1 cut(s) 365
BsoBI CYCGRG 1 cut(s) 1491
Bsp119I TTCGAA 2 cut(s) 773, 1305
Bsp1286I GDGCHC 2 cut(s) 184, 1810
Bsp19I CCATGG 1 cut(s) 261
Bsp68I TCGCGA 1 cut(s) 1951
BspACI CCGC 4 cut(s) 1068, 1121, 1129, 1192
BspANI GGCC 2 cut(s) 994, 1823
BspCNI CTCAG 3 cut(s) 68, 1165, 1867
BspDI ATCGAT 2 cut(s) 450, 1294
BspFNI CGCG 1 cut(s) 1951
BspLI GGNNCC 2 cut(s) 267, 1557
BspPI GGATC 5 cut(s) 125, 206, 1721, 1811, 1942
BspQI GCTCTTC 1 cut(s) 15
BspT104I TTCGAA 2 cut(s) 773, 1305
BspTNI GGTCTC 1 cut(s) 365
BsrFI RCCGGY 1 cut(s) 1513
BsrI ACTGG 3 cut(s) 178, 328, 811
BssAI RCCGGY 1 cut(s) 1513
BssECI CCNNGG 2 cut(s) 261, 1466
BssSI CACGAG 3 cut(s) 1505, 1525, 1938
BssT1I CCWWGG 1 cut(s) 261
Bst2BI CACGAG 3 cut(s) 1505, 1525, 1938
Bst2UI CCWGG 1 cut(s) 288
Bst4CI ACNGT 3 cut(s) 335, 1671, 1833
Bst6I CTCTTC 5 cut(s) 15, 116, 368, 950, 1335
BstBI TTCGAA 2 cut(s) 773, 1305
BstC8I GCNNGC 3 cut(s) 1546, 1581, 1949
BstDSI CCRYGG 1 cut(s) 261
BstEII GGTNACC 1 cut(s) 383
BstFNI CGCG 1 cut(s) 1951
BstMAI GTCTC 4 cut(s) 109, 365, 1834, 2025
BstMWI GCNNNNNNNGC 4 cut(s) 550, 1135, 1198, 1798
BstNI CCWGG 1 cut(s) 288
BstPAI GACNNNNGTC 1 cut(s) 2005
BstPI GGTNACC 1 cut(s) 383
BstSCI CCNGG 1 cut(s) 286
BstSFI CTRYAG 1 cut(s) 720
BstSLI GKGCMC 1 cut(s) 1810
BstUI CGCG 1 cut(s) 1951
BstV1I GCAGC 2 cut(s) 703, 1801
BstV2I GAAGAC 2 cut(s) 295, 1651
BstX2I RGATCY 4 cut(s) 211, 747, 1878, 1934
BstXI CCANNNNNNTGG 1 cut(s) 1531
BstYI RGATCY 4 cut(s) 211, 747, 1878, 1934
Bsu15I ATCGAT 2 cut(s) 450, 1294
Bsu36I CCTNAGG 1 cut(s) 831
BsuRI GGCC 2 cut(s) 994, 1823
BsuTUI ATCGAT 2 cut(s) 450, 1294
BtgI CCRYGG 1 cut(s) 261
BtsI GCAGTG 1 cut(s) 711
BtsIMutI CAGTG 2 cut(s) 321, 711
BtuMI TCGCGA 1 cut(s) 1951
Cac8I GCNNGC 3 cut(s) 1546, 1581, 1949
Cfr10I RCCGGY 1 cut(s) 1513
Cfr13I GGNCC 1 cut(s) 1521
ClaI ATCGAT 2 cut(s) 450, 1294
Csp6I GTAC 3 cut(s) 204, 660, 1539
CspAI ACCGGT 1 cut(s) 1513
CviAII CATG 7 cut(s) 262, 461, 1255, 1273, 1391, 1499, 1914
CviQI GTAC 3 cut(s) 204, 660, 1539
EaeI YGGCCR 1 cut(s) 1821
Eam1104I CTCTTC 5 cut(s) 15, 116, 368, 950, 1335
EarI CTCTTC 5 cut(s) 15, 116, 368, 950, 1335
Eco130I CCWWGG 1 cut(s) 261
Eco31I GGTCTC 1 cut(s) 365
Eco32I GATATC 1 cut(s) 1179
Eco47I GGWCC 1 cut(s) 1521
Eco57I CTGAAG 3 cut(s) 1356, 1581, 1988
Eco81I CCTNAGG 1 cut(s) 831
Eco88I CYCGRG 1 cut(s) 1491
Eco91I GGTNACC 1 cut(s) 383
EcoO65I GGTNACC 1 cut(s) 383
EcoRI GAATTC 1 cut(s) 1347
EcoRII CCWGG 1 cut(s) 286
EcoRV GATATC 1 cut(s) 1179
EcoT14I CCWWGG 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 261
FaeI CATG 7 cut(s) 265, 464, 1258, 1276, 1394, 1502, 1917
FatI CATG 7 cut(s) 261, 460, 1254, 1272, 1390, 1498, 1913
FauI CCCGC 1 cut(s) 1061
FbaI TGATCA 1 cut(s) 798
Fnu4HI GCNGC 2 cut(s) 717, 1790
Fsp4HI GCNGC 2 cut(s) 717, 1790
FspBI CTAG 3 cut(s) 339, 390, 1434
GluI GCNGC 2 cut(s) 717, 1790
HaeIII GGCC 2 cut(s) 994, 1823
HapII CCGG 4 cut(s) 1026, 1204, 1514, 1820
Hin1II CATG 7 cut(s) 265, 464, 1258, 1276, 1394, 1502, 1917
HindIII AAGCTT 1 cut(s) 551
HpaII CCGG 4 cut(s) 1026, 1204, 1514, 1820
HphI GGTGA 4 cut(s) 250, 940, 1718, 1877
Hpy166II GTNNAC 2 cut(s) 812, 1808
Hpy188I TCNGA 6 cut(s) 467, 523, 679, 738, 1002, 1983
Hpy188III TCNNGA 7 cut(s) 115, 434, 1374, 1559, 1625, 1814, 1950
Hpy8I GTNNAC 2 cut(s) 812, 1808
Hpy99I CGWCG 3 cut(s) 1154, 1837, 1987
HpyAV CCTTC 6 cut(s) 825, 1231, 1388, 1652, 1856, 2045
HpyCH4III ACNGT 3 cut(s) 335, 1671, 1833
HpyCH4IV ACGT 1 cut(s) 662
HpyCH4V TGCA 8 cut(s) 170, 314, 619, 1233, 1390, 1583, 1789, 1808
HpyF10VI GCNNNNNNNGC 4 cut(s) 550, 1135, 1198, 1798
HpySE526I ACGT 1 cut(s) 662
Hsp92II CATG 7 cut(s) 265, 464, 1258, 1276, 1394, 1502, 1917
Ksp22I TGATCA 1 cut(s) 798
LguI GCTCTTC 1 cut(s) 15
LmnI GCTCC 2 cut(s) 271, 1561
Lsp1109I GCAGC 2 cut(s) 703, 1801
LweI GCATC 2 cut(s) 442, 1592
MaeI CTAG 3 cut(s) 339, 390, 1434
MaeII ACGT 1 cut(s) 662
MaeIII GTNAC 6 cut(s) 290, 383, 443, 610, 1095, 1753
MflI RGATCY 4 cut(s) 211, 747, 1878, 1934
MhlI GDGCHC 2 cut(s) 184, 1810
MluCI AATT 9 cut(s) 367, 437, 468, 478, 769, 775, 1207, 1347, 2043
MlyI GAGTC 6 cut(s) 83, 748, 830, 1192, 1412, 1919
MmeI TCCRAC 3 cut(s) 445, 685, 2006
MslI CAYNNNNRTG 1 cut(s) 1529
MspA1I CMGCKG 1 cut(s) 1544
MspI CCGG 4 cut(s) 1026, 1204, 1514, 1820
MspR9I CCNGG 1 cut(s) 288
MvaI CCWGG 1 cut(s) 288
MvnI CGCG 1 cut(s) 1951
MwoI GCNNNNNNNGC 4 cut(s) 550, 1135, 1198, 1798
NcoI CCATGG 1 cut(s) 261
NlaIII CATG 7 cut(s) 265, 464, 1258, 1276, 1394, 1502, 1917
NlaIV GGNNCC 2 cut(s) 267, 1557
NmuCI GTSAC 2 cut(s) 290, 443
NruI TCGCGA 1 cut(s) 1951
NspV TTCGAA 2 cut(s) 773, 1305
OliI CACNNNNGTG 1 cut(s) 1529
PciSI GCTCTTC 1 cut(s) 15
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 779
PflMI CCANNNNNTGG 1 cut(s) 323
PinAI ACCGGT 1 cut(s) 1513
PkrI GCNGC 2 cut(s) 718, 1791
PleI GAGTC 6 cut(s) 83, 747, 830, 1191, 1411, 1918
PpsI GAGTC 6 cut(s) 83, 747, 830, 1191, 1411, 1918
PshAI GACNNNNGTC 1 cut(s) 2005
PshBI ATTAAT 1 cut(s) 1694
PsiI TTATAA 1 cut(s) 1086
Psp6I CCWGG 1 cut(s) 286
PspEI GGTNACC 1 cut(s) 383
PspGI CCWGG 1 cut(s) 286
PspN4I GGNNCC 2 cut(s) 267, 1557
PspPI GGNCC 1 cut(s) 1521
PsrI GAACNNNNNNTAC 2 cut(s) 187, 219
PsuI RGATCY 4 cut(s) 211, 747, 1878, 1934
PvuII CAGCTG 1 cut(s) 1544
RruI TCGCGA 1 cut(s) 1951
RsaI GTAC 3 cut(s) 205, 661, 1540
RsaNI GTAC 3 cut(s) 204, 660, 1539
RseI CAYNNNNRTG 1 cut(s) 1529
SapI GCTCTTC 1 cut(s) 15
SatI GCNGC 2 cut(s) 717, 1790
Sau96I GGNCC 1 cut(s) 1521
SchI GAGTC 6 cut(s) 83, 748, 830, 1192, 1412, 1919
ScrFI CCNGG 1 cut(s) 288
SduI GDGCHC 2 cut(s) 184, 1810
SfaNI GCATC 2 cut(s) 442, 1592
SfcI CTRYAG 1 cut(s) 720
SfuI TTCGAA 2 cut(s) 773, 1305
SinI GGWCC 1 cut(s) 1521
SmiMI CAYNNNNRTG 1 cut(s) 1529
Sse9I AATT 9 cut(s) 367, 437, 468, 478, 769, 775, 1207, 1347, 2043
SsiI CCGC 4 cut(s) 1068, 1121, 1129, 1192
SspI AATATT 1 cut(s) 1380
SspMI CTAG 3 cut(s) 339, 390, 1434
StyD4I CCNGG 1 cut(s) 286
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 3 cut(s) 335, 1671, 1833
TaiI ACGT 1 cut(s) 665
TaqII GACCGA 2 cut(s) 1467, 1610
TasI AATT 9 cut(s) 367, 437, 468, 478, 769, 775, 1207, 1347, 2043
TscAI CASTG 2 cut(s) 328, 718
TseFI GTSAC 2 cut(s) 290, 443
TseI GCWGC 2 cut(s) 716, 1789
Tsp45I GTSAC 2 cut(s) 290, 443
TspDTI ATGAA 9 cut(s) 17, 58, 241, 476, 1243, 1407, 1487, 1785, 1940
TspGWI ACGGA 3 cut(s) 191, 309, 996
TspRI CASTG 2 cut(s) 328, 718
Van91I CCANNNNNTGG 1 cut(s) 323
VneI GTGCAC 1 cut(s) 1806
VpaK11BI GGWCC 1 cut(s) 1521
VspI ATTAAT 1 cut(s) 1694
XapI RAATTY 4 cut(s) 437, 775, 1207, 1347
XspI CTAG 3 cut(s) 339, 390, 1434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.