Rh5BG062800

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0011839
Physical Location & Seq
Forward (+)
5142649 .. 5157666
15018 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG062800.1

Sequence Viewer

Length: 2127 bp
ATGAAAACCCTTCTCATCTTTCTTCTCCTCCTCTCCCTCCGACCCCTCTTCGCCGTCGATTTCATCTTCAACGGCTTTAACTCCTCCTCCAACCTCCTCCTCTTCGGCAACGCCACCGTCTCATCTCAAATCCTCACTATCACAAACGCCACCAGCTACTCCATCGGCCGAGCTCTCTACAAGTCCAAAATCCCAACCAAAGCTCCGAACTCTTCATACATCTACCCTTTCTCGACCTCCTTCGTCTTCTCCATCGCTCCTGACAAGAACACTCTCCCCGGCCACGGCATGGTCTTCATCTTCACGCCCGCCGAGGGCATAAACGGAACCAGCGCCAGCGAGCATTTAGGGTTGCTCAACTTCAAAAACAGAGGCCAGTCGACGAACCATGTTTTTGGGGTCGAGTTCGATGTGTTTAAGAACGAGGAGTTTAAGGACATTAGTAACAACCACGTCGGAATTGATGTGAACTCATTGGAGTCGGTTAATGCATCCGAAGCCGGGTATTGGAGTGAGGATAAGGTTTTCAAGAAGTTGAAGCTCAACAATGGTGAGAATTACCAAGCTTGGATTGATTATAAGGATTCGGTGATTAATGTGACTATGGTGAAAGCTGGGATGGCGAAGCCGAAGACTCCGTTGTTGAGTTATGTTTATAATCTTTCTGATGTTTTGGAGGATGAAATGTTTGTCGGCTTCACAGGCTCAACGGGGCAATTAGTCGAAAGTCACATGATTTTGGCTTGGAGTTTCAGCAATACTAATTTTTCTTTGGGTGAGAGTTTGATCACTTCTGGCTTGCCTAATTTTGTGCTTCCTGAGGCTTCTATTTTTAAGTCTAAAGGGTTTATTGCTGGAGTTACAGTTGGGGGTTTGTTTGTAGTTTGTGTGATTGCTCTGTTTACTCTGTTTTTGGTTAAGAGAAAAAGGAGGATGAAAAGGGAGAGAGATGAAATGGAGGATTGGGAATTGGAGTACTGGCCTCACCGGATTACTTATTCGGAAATTGAGGCGGCGACGAAGGGGTTTCGGGAAGAAAATGTGATTGGAATTGGAGGGAATGGGAAGGTGTACAAGGGTGTTTTGGCAGGAGGGGCAGAGGTTGCGGTGAAGAGAATTTCACATGAGAATAATGGAATGAGAGAGTTCTTGGCTGAGATTTCAAGCATTGGGAGATTGAAGCATAGAAATTTGGTGGGATTGAGAGGTTGGTGTAAGAAAGAGAAGGGGAATTTCATGTTGGTCTATGATTATATGGAAAATGGGAGTTTGGATAAGTTGATTTTTGATTGTGATGAGAGTAAGATGTTGGGGTTTGAAGACAGGATTAGGGTTTTGAGAGATGTGGCTTCTGGGGTTTTGTACTTACATGAAGGATGGGAATCTAAAGTGTTGCATAGGGACATTAAGGCAAGCAATGTGTTACTTGATAGGGAAATGAATGGAAGGTTAGGGGATTTCGGGTTGGCGAGGATGCACGGGCATGGTGATGTGCCCGGCACTACTCGGGTGGTAGGAACCGTGGGGTACTTGGCCCCGGAAATCATTCACGTAGGGCGAGCGTCCGCCCAGACTGACGTTTTCGGGTTCGGGATCTTGATTTTAGAAGTGATGTGTGGGAGGAGGCCTATAGAGGAAGGGAAGCCACCCTTGGTGGAATGGGTGTGGCAACTCATGGTGAAAGGGCAATTGCTAAATGCGTTTGATGAGAGGTTGAGGGGCAGAGGTTTGGGGTTAAGTGAAGAGGAGGTGGAGAGGGTTTTACATTTGGGTTTGTTGTGTGCTTACCCAGAGCCAAATGCAAGACCATCAATGAGGCAAGTGGTGAAAGTTTTGGAGGGGAAGAATGACAATGAGGGCTGCGATGAGTCTGAAACTGAGGAAATGGATGCTTATTTGCTTCAGAAATTGCAATCCAAGGACATGTGGTCTGAGTTTTCACATAATTTTGGTTATGGATTATCATCACACCCAACTATTCAAGATATCAAGCAGTCAATATCCAACTCAATGTCTCTCTCTTGGTCCAGTACTAATATAGTGGAGGGTACATCGGGCTTGCAATGCATACCTAGCTACGTGGCTGGATGCCTCAAGCTAGATGATCAGAGTTGTCTCTCTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lectin_legB Pkinase Pkinase_Tyr

Protein Analysis

708

Amino Acids

78.84

Weight (kDa)

5.89

Isoelectric Point (pI)

30.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 19 - 255 6.6e-69 Legume lectin domain
Lectin_L-type_dom PF18483 30 - 244 7.8e-11 Legume lectin beta-barrel domain
Pkinase PF00069 344 - 609 5.8e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 346 - 612 4.9e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011839)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G04960 AT4G04960
fragaria_vesca FvH4_3g09090
malus_domestica MD05G1338700.v1.1 MD10G1293700.v1.1
prunus_persica Prupe.4G048100_v2.0.a1
pyrus_communis pycom05g29000 pycom10g24560
rosa_chinensis RchiOBHm_Chr5g0008551
rosa_laevigata RLG00000031579 RLG00000031582
rosa_multiflora Rmu_sc0001665.1_g000016
rosa_roxburghii Rroxscaffold_1G00068000
rosa_rugosa Rorug04G0436600
rosa_samantha Rh5AG065400 Rh5BG062800 Rh5CG073000 Rh5DG063300
rosa_wichuraiana Rw5G005550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 579, 657
AccB7I CCANNNNNTGG 1 cut(s) 289
AccI GTMKAC 1 cut(s) 380
AciI CCGC 4 cut(s) 309, 1013, 1106, 1566
AclWI GGATC 1 cut(s) 1601
AcoI YGGCCR 2 cut(s) 166, 280
AcsI RAATTY 3 cut(s) 1116, 1189, 1231
AcuI CTGAAG 1 cut(s) 1886
AfaI GTAC 6 cut(s) 977, 1073, 1364, 1529, 2032, 2050
AfiI CCNNNNNNNGG 5 cut(s) 284, 289, 314, 501, 507
AflIII ACRYGT 1 cut(s) 1923
AgsI TTSAA 8 cut(s) 70, 364, 529, 538, 1164, 1180, 1319, 1982
AhdI GACNNNNNGTC 1 cut(s) 1927
AjiI CACGTC 1 cut(s) 454
AjuI GAANNNNNNNTTGG 6 cut(s) 1029, 1061, 1223, 1255, 1634, 1666
AluBI AGCT 8 cut(s) 156, 173, 203, 541, 566, 614, 2076, 2098
AluI AGCT 8 cut(s) 156, 173, 203, 541, 566, 614, 2076, 2098
Alw21I GWGCWC 1 cut(s) 175
Alw26I GTCTC 3 cut(s) 124, 2019, 2120
AlwI GGATC 1 cut(s) 1601
Ama87I CYCGRG 1 cut(s) 1506
AoxI GGCC 6 cut(s) 166, 280, 373, 980, 1533, 1625
ApeKI GCWGC 1 cut(s) 1860
ApoI RAATTY 3 cut(s) 1116, 1189, 1231
AseI ATTAAT 1 cut(s) 594
Asp700I GAANNNNTTC 1 cut(s) 1545
AspLEI GCGC 1 cut(s) 335
AspS9I GGNCC 2 cut(s) 1534, 2025
AsuC2I CCSGG 4 cut(s) 279, 502, 1497, 1538
AsuHPI GGTGA 9 cut(s) 563, 601, 619, 788, 977, 1120, 1499, 1690, 1837
AvaI CYCGRG 1 cut(s) 1506
AvaII GGWCC 1 cut(s) 2025
AxyI CCTNAGG 1 cut(s) 819
BaeGI GKGCMC 1 cut(s) 1497
BanII GRGCYC 1 cut(s) 175
BbsI GAAGAC 4 cut(s) 238, 286, 638, 1326
Bbv12I GWGCWC 1 cut(s) 175
BbvI GCAGC 1 cut(s) 1847
BccI CCATC 5 cut(s) 170, 260, 613, 1371, 1816
BceAI ACGGC 3 cut(s) 38, 88, 301
BclI TGATCA 2 cut(s) 786, 2104
BcnI CCSGG 4 cut(s) 279, 502, 1497, 1538
BcoDI GTCTC 3 cut(s) 124, 2019, 2120
BfaI CTAG 2 cut(s) 2073, 2099
BfmI CTRYAG 1 cut(s) 1629
BfoI RGCGCY 1 cut(s) 336
BisI GCNGC 2 cut(s) 1014, 1861
BlsI GCNGC 2 cut(s) 1015, 1862
BmcAI AGTACT 2 cut(s) 977, 2032
Bme1390I CCNGG 4 cut(s) 279, 502, 1497, 1538
Bme18I GGWCC 1 cut(s) 2025
BmeRI GACNNNNNGTC 1 cut(s) 1927
BmeT110I CYCGRG 1 cut(s) 1506
BmgBI CACGTC 1 cut(s) 454
BmgT120I GGNCC 2 cut(s) 1534, 2025
BmiI GGNNCC 3 cut(s) 328, 1519, 1536
BmrFI CCNGG 4 cut(s) 279, 502, 1497, 1538
BmsI GCATC 4 cut(s) 500, 1464, 1879, 2078
BpiI GAAGAC 4 cut(s) 238, 286, 638, 1326
BpmI CTGGAG 1 cut(s) 876
BpuEI CTTGAG 1 cut(s) 2078
BpuMI CCSGG 4 cut(s) 279, 502, 1497, 1538
BsaAI YACGTR 2 cut(s) 1552, 2080
BsaBI GATNNNNATC 2 cut(s) 1381, 1963
BsaJI CCNNGG 7 cut(s) 277, 283, 312, 1521, 1536, 1650, 1917
BsaWI WCCGGW 1 cut(s) 987
BsaXI ACNNNNNCTCC 6 cut(s) 71, 101, 187, 217, 849, 879
Bsc4I CCNNNNNNNGG 5 cut(s) 284, 289, 314, 501, 507
Bse1I ACTGG 3 cut(s) 376, 983, 2028
Bse21I CCTNAGG 1 cut(s) 819
Bse3DI GCAATG 2 cut(s) 1423, 2069
Bse8I GATNNNNATC 2 cut(s) 1381, 1963
BseDI CCNNGG 7 cut(s) 277, 283, 312, 1521, 1536, 1650, 1917
BseGI GGATG 8 cut(s) 491, 624, 685, 939, 1382, 1479, 1894, 2093
BseJI GATNNNNATC 2 cut(s) 1381, 1963
BseLI CCNNNNNNNGG 5 cut(s) 284, 289, 314, 501, 507
BseMI GCAATG 2 cut(s) 1423, 2069
BseMII CTCAG 4 cut(s) 810, 1146, 1869, 1923
BseNI ACTGG 3 cut(s) 376, 983, 2028
BseRI GAGGAG 9 cut(s) 17, 20, 73, 76, 86, 89, 440, 1636, 1760
BseSI GKGCMC 1 cut(s) 1497
BseX3I CGGCCG 1 cut(s) 166
BseXI GCAGC 1 cut(s) 1847
BseYI CCCAGC 1 cut(s) 614
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 6 cut(s) 168, 282, 375, 982, 1535, 1627
BsiEI CGRYCG 1 cut(s) 169
BsiHKAI GWGCWC 1 cut(s) 175
BsiHKCI CYCGRG 1 cut(s) 1506
BsiSI CCGG 5 cut(s) 279, 501, 988, 1497, 1538
BslFI GGGAC 1 cut(s) 1415
BslI CCNNNNNNNGG 5 cut(s) 284, 289, 314, 501, 507
BsmAI GTCTC 3 cut(s) 124, 2019, 2120
BsmBI CGTCTC 1 cut(s) 124
BsmFI GGGAC 1 cut(s) 1415
BsnI GGCC 6 cut(s) 168, 282, 375, 982, 1535, 1627
BsoBI CYCGRG 1 cut(s) 1506
Bsp1286I GDGCHC 2 cut(s) 175, 1497
Bsp1407I TGTACA 1 cut(s) 1071
Bsp143I GATC 3 cut(s) 786, 1593, 2104
BspACI CCGC 4 cut(s) 309, 1013, 1106, 1566
BspANI GGCC 6 cut(s) 168, 282, 375, 982, 1535, 1627
BspCNI CTCAG 4 cut(s) 811, 1147, 1870, 1924
BspLI GGNNCC 3 cut(s) 328, 1519, 1536
BspPI GGATC 1 cut(s) 1601
BsrDI GCAATG 2 cut(s) 1423, 2069
BsrGI TGTACA 1 cut(s) 1071
BsrI ACTGG 3 cut(s) 376, 983, 2028
BssECI CCNNGG 7 cut(s) 277, 283, 312, 1521, 1536, 1650, 1917
BssMI GATC 3 cut(s) 786, 1593, 2104
BssT1I CCWWGG 2 cut(s) 1650, 1917
Bst4CI ACNGT 3 cut(s) 118, 865, 1522
Bst6I CTCTTC 5 cut(s) 53, 107, 217, 1106, 1737
BstAPI GCANNNNNTGC 1 cut(s) 1103
BstAUI TGTACA 1 cut(s) 1071
BstBAI YACGTR 2 cut(s) 1552, 2080
BstC8I GCNNGC 7 cut(s) 309, 337, 341, 800, 1414, 1560, 2060
BstDEI CTNAG 4 cut(s) 819, 1155, 1878, 1932
BstDSI CCRYGG 2 cut(s) 283, 1521
BstF5I GGATG 8 cut(s) 491, 624, 685, 939, 1382, 1479, 1894, 2093
BstH2I RGCGCY 1 cut(s) 336
BstHHI GCGC 1 cut(s) 335
BstKTI GATC 3 cut(s) 789, 1596, 2107
BstMAI GTCTC 3 cut(s) 124, 2019, 2120
BstMBI GATC 3 cut(s) 786, 1593, 2104
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 7 cut(s) 497, 620, 702, 1094, 1103, 2064, 2073
BstNSI RCATGY 1 cut(s) 1927
BstSCI CCNGG 4 cut(s) 277, 500, 1495, 1536
BstSFI CTRYAG 1 cut(s) 1629
BstSLI GKGCMC 1 cut(s) 1497
BstV1I GCAGC 1 cut(s) 1847
BstV2I GAAGAC 4 cut(s) 238, 286, 638, 1326
BstX2I RGATCY 1 cut(s) 1593
BstXI CCANNNNNNTGG 1 cut(s) 395
BstYI RGATCY 1 cut(s) 1593
BstZI CGGCCG 1 cut(s) 166
Bsu36I CCTNAGG 1 cut(s) 819
BsuRI GGCC 6 cut(s) 168, 282, 375, 982, 1535, 1627
BtgI CCRYGG 2 cut(s) 283, 1521
BtgZI GCGATG 2 cut(s) 238, 1878
BtrI CACGTC 1 cut(s) 454
BtsCI GGATG 8 cut(s) 491, 624, 685, 939, 1382, 1479, 1894, 2093
Cac8I GCNNGC 7 cut(s) 309, 337, 341, 800, 1414, 1560, 2060
CfoI GCGC 1 cut(s) 335
Cfr13I GGNCC 2 cut(s) 1534, 2025
CseI GACGC 1 cut(s) 1551
Csp6I GTAC 6 cut(s) 976, 1072, 1363, 1528, 2031, 2049
CviAII CATG 9 cut(s) 289, 389, 733, 1124, 1237, 1370, 1484, 1675, 1924
CviQI GTAC 6 cut(s) 976, 1072, 1363, 1528, 2031, 2049
DdeI CTNAG 4 cut(s) 819, 1155, 1878, 1932
DpnI GATC 3 cut(s) 788, 1595, 2106
DpnII GATC 3 cut(s) 786, 1593, 2104
DriI GACNNNNNGTC 1 cut(s) 1927
EaeI YGGCCR 2 cut(s) 166, 280
EagI CGGCCG 1 cut(s) 166
Eam1104I CTCTTC 5 cut(s) 53, 107, 217, 1106, 1737
Eam1105I GACNNNNNGTC 1 cut(s) 1927
EarI CTCTTC 5 cut(s) 53, 107, 217, 1106, 1737
EciI GGCGGA 1 cut(s) 1555
Ecl136II GAGCTC 1 cut(s) 173
EclXI CGGCCG 1 cut(s) 166
Eco130I CCWWGG 2 cut(s) 1650, 1917
Eco147I AGGCCT 1 cut(s) 1627
Eco24I GRGCYC 1 cut(s) 175
Eco32I GATATC 1 cut(s) 1987
Eco47I GGWCC 1 cut(s) 2025
Eco52I CGGCCG 1 cut(s) 166
Eco53kI GAGCTC 1 cut(s) 173
Eco57I CTGAAG 1 cut(s) 1886
Eco81I CCTNAGG 1 cut(s) 819
Eco88I CYCGRG 1 cut(s) 1506
EcoICRI GAGCTC 1 cut(s) 173
EcoRV GATATC 1 cut(s) 1987
EcoT14I CCWWGG 2 cut(s) 1650, 1917
EcoT22I ATGCAT 2 cut(s) 493, 2069
EcoT38I GRGCYC 1 cut(s) 175
ErhI CCWWGG 2 cut(s) 1650, 1917
Esp3I CGTCTC 1 cut(s) 124
FaeI CATG 9 cut(s) 292, 392, 736, 1127, 1240, 1373, 1487, 1678, 1927
FalI AAGNNNNNCTT 2 cut(s) 1634, 1666
FaqI GGGAC 1 cut(s) 1415
FatI CATG 9 cut(s) 288, 388, 732, 1123, 1236, 1369, 1483, 1674, 1923
FauI CCCGC 1 cut(s) 316
FbaI TGATCA 2 cut(s) 786, 2104
FblI GTMKAC 1 cut(s) 380
Fnu4HI GCNGC 2 cut(s) 1014, 1861
FokI GGATG 8 cut(s) 478, 631, 692, 946, 1389, 1486, 1901, 2100
FriOI GRGCYC 1 cut(s) 175
Fsp4HI GCNGC 2 cut(s) 1014, 1861
FspBI CTAG 2 cut(s) 2073, 2099
GlaI GCGC 1 cut(s) 334
GluI GCNGC 2 cut(s) 1014, 1861
GsaI CCCAGC 1 cut(s) 618
GsuI CTGGAG 1 cut(s) 876
HaeII RGCGCY 1 cut(s) 336
HaeIII GGCC 6 cut(s) 168, 282, 375, 982, 1535, 1627
HapII CCGG 5 cut(s) 279, 501, 988, 1497, 1538
HgaI GACGC 1 cut(s) 1551
HhaI GCGC 1 cut(s) 335
Hin1II CATG 9 cut(s) 292, 392, 736, 1127, 1240, 1373, 1487, 1678, 1927
Hin6I GCGC 1 cut(s) 333
HinP1I GCGC 1 cut(s) 333
HincII GTYRAC 1 cut(s) 381
HindII GTYRAC 1 cut(s) 381
HindIII AAGCTT 1 cut(s) 564
HinfI GANTC 5 cut(s) 479, 584, 634, 1382, 1868
HpaII CCGG 5 cut(s) 279, 501, 988, 1497, 1538
HphI GGTGA 9 cut(s) 563, 601, 619, 788, 977, 1120, 1499, 1690, 1837
Hpy166II GTNNAC 4 cut(s) 381, 469, 903, 1072
Hpy188III TCNNGA 8 cut(s) 232, 260, 529, 818, 1031, 1591, 1597, 1982
Hpy8I GTNNAC 4 cut(s) 381, 469, 903, 1072
Hpy99I CGWCG 4 cut(s) 59, 385, 458, 1021
HpyAV CCTTC 8 cut(s) 20, 250, 1015, 1060, 1219, 1367, 1440, 1631
HpyCH4III ACNGT 3 cut(s) 118, 865, 1522
HpyCH4IV ACGT 4 cut(s) 453, 1551, 1578, 2079
HpyCH4V TGCA 7 cut(s) 491, 1396, 1477, 1802, 1912, 2062, 2067
HpyF10VI GCNNNNNNNGC 7 cut(s) 497, 620, 702, 1094, 1103, 2064, 2073
HpyF3I CTNAG 4 cut(s) 819, 1155, 1878, 1932
HpySE526I ACGT 4 cut(s) 453, 1551, 1578, 2079
Hsp92II CATG 9 cut(s) 292, 392, 736, 1127, 1240, 1373, 1487, 1678, 1927
HspAI GCGC 1 cut(s) 333
Ksp22I TGATCA 2 cut(s) 786, 2104
Kzo9I GATC 3 cut(s) 786, 1593, 2104
LmnI GCTCC 2 cut(s) 208, 262
Lsp1109I GCAGC 1 cut(s) 1847
LweI GCATC 4 cut(s) 500, 1464, 1879, 2078
MaeI CTAG 2 cut(s) 2073, 2099
MaeII ACGT 4 cut(s) 453, 1551, 1578, 2079
MaeIII GTNAC 5 cut(s) 443, 598, 728, 859, 1422
MalI GATC 3 cut(s) 788, 1595, 2106
MboI GATC 3 cut(s) 786, 1593, 2104
MfeI CAATTG 1 cut(s) 1688
MflI RGATCY 1 cut(s) 1593
MhlI GDGCHC 2 cut(s) 175, 1497
MlyI GAGTC 3 cut(s) 488, 628, 1877
MmeI TCCRAC 4 cut(s) 64, 114, 436, 2028
Mph1103I ATGCAT 2 cut(s) 493, 2069
MroXI GAANNNNTTC 1 cut(s) 1545
MseI TTAA 9 cut(s) 78, 417, 432, 486, 594, 834, 918, 1407, 1736
MslI CAYNNNNRTG 2 cut(s) 1482, 1488
MspI CCGG 5 cut(s) 279, 501, 988, 1497, 1538
MspR9I CCNGG 4 cut(s) 279, 502, 1497, 1538
MunI CAATTG 1 cut(s) 1688
MwoI GCNNNNNNNGC 7 cut(s) 497, 620, 702, 1094, 1103, 2064, 2073
NciI CCSGG 4 cut(s) 279, 502, 1497, 1538
NdeII GATC 3 cut(s) 786, 1593, 2104
NlaIII CATG 9 cut(s) 292, 392, 736, 1127, 1240, 1373, 1487, 1678, 1927
NlaIV GGNNCC 3 cut(s) 328, 1519, 1536
NmeAIII GCCGAG 2 cut(s) 194, 337
NmuCI GTSAC 2 cut(s) 598, 728
NsiI ATGCAT 2 cut(s) 493, 2069
NspI RCATGY 1 cut(s) 1927
PceI AGGCCT 1 cut(s) 1627
PciI ACATGT 1 cut(s) 1923
PdmI GAANNNNTTC 1 cut(s) 1545
PfeI GAWTC 2 cut(s) 584, 1382
PflMI CCANNNNNTGG 1 cut(s) 289
PkrI GCNGC 2 cut(s) 1015, 1862
PleI GAGTC 3 cut(s) 487, 628, 1876
PpsI GAGTC 3 cut(s) 487, 628, 1876
Ppu21I YACGTR 2 cut(s) 1552, 2080
PscI ACATGT 1 cut(s) 1923
PshBI ATTAAT 1 cut(s) 594
PsiI TTATAA 2 cut(s) 579, 657
Psp124BI GAGCTC 1 cut(s) 175
PspFI CCCAGC 1 cut(s) 614
PspN4I GGNNCC 3 cut(s) 328, 1519, 1536
PspPI GGNCC 2 cut(s) 1534, 2025
PsrI GAACNNNNNNTAC 2 cut(s) 200, 232
PsuI RGATCY 1 cut(s) 1593
RsaI GTAC 6 cut(s) 977, 1073, 1364, 1529, 2032, 2050
RsaNI GTAC 6 cut(s) 976, 1072, 1363, 1528, 2031, 2049
RseI CAYNNNNRTG 2 cut(s) 1482, 1488
SacI GAGCTC 1 cut(s) 175
SalI GTCGAC 1 cut(s) 379
SaqAI TTAA 9 cut(s) 78, 417, 432, 486, 594, 834, 918, 1407, 1736
SatI GCNGC 2 cut(s) 1014, 1861
Sau3AI GATC 3 cut(s) 786, 1593, 2104
Sau96I GGNCC 2 cut(s) 1534, 2025
ScaI AGTACT 2 cut(s) 977, 2032
SchI GAGTC 3 cut(s) 488, 628, 1877
ScrFI CCNGG 4 cut(s) 279, 502, 1497, 1538
SduI GDGCHC 2 cut(s) 175, 1497
SfaNI GCATC 4 cut(s) 500, 1464, 1879, 2078
SfcI CTRYAG 1 cut(s) 1629
SinI GGWCC 1 cut(s) 2025
SmiMI CAYNNNNRTG 2 cut(s) 1482, 1488
SmlI CTYRAG 1 cut(s) 2093
SmoI CTYRAG 1 cut(s) 2093
SseBI AGGCCT 1 cut(s) 1627
SsiI CCGC 4 cut(s) 309, 1013, 1106, 1566
SspMI CTAG 2 cut(s) 2073, 2099
SstI GAGCTC 1 cut(s) 175
StuI AGGCCT 1 cut(s) 1627
StyD4I CCNGG 4 cut(s) 277, 500, 1495, 1536
StyI CCWWGG 2 cut(s) 1650, 1917
TaaI ACNGT 3 cut(s) 118, 865, 1522
TaiI ACGT 4 cut(s) 456, 1554, 1581, 2082
TaqI TCGA 6 cut(s) 57, 233, 380, 402, 408, 723
TatI WGTACW 4 cut(s) 975, 1071, 1362, 2030
TauI GCSGC 1 cut(s) 1016
TfiI GAWTC 2 cut(s) 584, 1382
Tru1I TTAA 9 cut(s) 78, 417, 432, 486, 594, 834, 918, 1407, 1736
Tru9I TTAA 9 cut(s) 78, 417, 432, 486, 594, 834, 918, 1407, 1736
TseFI GTSAC 2 cut(s) 598, 728
TseI GCWGC 1 cut(s) 1860
Tsp45I GTSAC 2 cut(s) 598, 728
TspGWI ACGGA 2 cut(s) 339, 627
Van91I CCANNNNNTGG 1 cut(s) 289
VpaK11BI GGWCC 1 cut(s) 2025
VspI ATTAAT 1 cut(s) 594
XapI RAATTY 3 cut(s) 1116, 1189, 1231
XceI RCATGY 1 cut(s) 1927
XmiI GTMKAC 1 cut(s) 380
XmnI GAANNNNTTC 1 cut(s) 1545
XspI CTAG 2 cut(s) 2073, 2099
ZrmI AGTACT 2 cut(s) 977, 2032
Zsp2I ATGCAT 2 cut(s) 493, 2069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.