Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008984

Physical Location & Seq

Genomic Coordinates Reverse (-)

7467845 .. 7470737

Gene Structure 2893 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G12650.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1959 bp

ATGTTTGGTGTGTATCGAGTATTCGTGTTACTGGTGTTTGTTAGTCAGCTTTGCAATGGGTTTTACTTACCAGGGAGTTATATGCACACATATTCAGATGGAGACTCTATCTTCGCCAAAGTCAATTCCTTGACTTCTATTGAAACTGAGCTTCCTTTCAGTTACTATAGTCTTCCTTACTGCCAACCACTTGAAGGTATCAAGAAAAGTGCTGAGAATCTTGGTGAGCTTCTCATGGGTGATCAGATTGATAACTCTGCTTATAGGTTTCGGATGAGAACCAATGAGTCTCTTTACTTATGCACTACGAGTCCTTTGAATGAGCATGAGGTCAAGCTCTTGAAACAGAGAACTCGTGAGTTGTATCAAGTGAATATGATTCTTGATAACTTGCCTGCTTTGAGATTCGCTAAGCAGAATGGGGTTACTATCCAGTGGACTGGATATCCTGTTGGGTATTCGCCACCTAACAGTAATGATGATTACATCATTAATCACCTCAAGTTTAAGGTTTTGGTTCATGAGTATGAAGGCAATGTCATGGAGGTGATTGGTACTGGTGAAGAAGGTATGGGTGTGATCTCTGAAGCTGATAAGAAGAAGGCATTGGGGTATGAGATTGTTGGATTTGAGGTTGTTCCCTGCAGTGTTAAGTATGATGCTGAAAAAATGACGAAGCTTCACATGTATGACCCTGTTCCTTCAGTTAACTGTCCATTGGAGCTTGACAAAGCTCAGATCATTAAAGAACATGAAAGAATAACCTTCACTTATGAAGTGGAATTTGTTAAGAGTGAGACAAGATGGCCATCCAGATGGGATGCCTATTTGAAAATGGAAGGTGCTCGTGTTCATTGGTTCTCTATTTTGAACTCGCTTATGGTGATCTTCTTTCTGGCCGGTATAGTTTTCGTCATATTCCTGAGGACAGTGAGAAGGGATTTGACAAAGTATGAGGAACTGGACAAGGAAGCACAGGCACAGATGAATGAGGAGCTCTCTGGATGGAAACTTGTTGTCGGAGATGTGTTCAGAGAACCAGAAATGTCAAAGCTTCTATGCATTATGGTGGGAGATGGGGTTCGAATTACAGGAATGGCTGTTGTCACTATTGTTTTCGCTGCACTTGGATTCATGTCGCCTGCCTCAAGAGGAATGCTACTAACCGGGATGATTATTCTCTATCTTTTCTTGGGTATTGTTGCTGGGTACGCTGGTGTTCGGCTCTGGAGAACTGTCAAGGGGACTTCAGAAGGATGGAGATCACTTTCCTGGTCCATTGCATGCTTCTTCCCGGGTATTGCTTTTGTTATACTCACAGTGTTGAACTTCCTTCTCTGGAGCAGCAACAGCACTGGAGCCATCCCAATCTCTTTGTATTTTGAGCTCTTGGCTCTCTGGTTCTGCATCTCGGTTCCTCTCACCCTATTTGGAGGATTCCTAGGAACACGAGCAGAAGCAATCCAGTTCCCAGTGAGAACCAACCAGATTCCAAGGGAAATCCCAGAACGGAAATACCCGTCATGGCTTCTAGTCCTTGGAGCAGGAACCCTTCCTTTTGGAACGCTCTTCATAGAACTGTTCTTCATCTTCTCCAGCATATGGTTAGGGAGATTCTACTATGTATTTGGGTTCTTACTCATAGTTCTGCTTCTTTTGGTAGTCGTGTGTGCAGAAGTATCAGTGGTCTTGACCTACATGCACCTCTGCGTTGAAGACTGGAGGTGGTGGTGGAAAGCCTTCTATGCATCTGGTTCAGTCGCATTGTACGTTTTCGCTTACTCCATCAACTATTTGGTCTTTGATCTACAAAGTCTGAGCGGACCCGTCTCAGCTATGCTTTACATTGGCTACTCTCTGCTTATGGCAATTGCAATCATGCTCGCCACTGGAACCATTGGCTTCCTCACCTCCTTTTACTTCGTCCACTATCTCTTTTCCTCTGTCAAAATCGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0005911 GO:0008150 GO:0009506 GO:0012505 GO:0016020 GO:0016021 GO:0030054 GO:0031090 GO:0031224 GO:0031410 GO:0031982 GO:0031984 GO:0032386 GO:0032388 GO:0032879 GO:0032880 GO:0033157 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048518 GO:0050789 GO:0051049 GO:0051050 GO:0051222 GO:0051223 GO:0055044 GO:0060341 GO:0065007 GO:0070201 GO:0070861 GO:0070863 GO:0090087 GO:0090316 GO:0097708 GO:0098588 GO:0098791 GO:0098805 GO:1903827 GO:1903829 GO:1904951 GO:2000008 GO:2000010

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EMP70

Protein Analysis

652

Amino Acids

74.14

Weight (kDa)

5.85

Isoelectric Point (pI)

38.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EMP70	PF02990	52 - 605	1.4e-178	Endomembrane protein 70

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008984)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G12650
fragaria_vesca	FvH4_6g40160 FvH4_6g40160 FvH4_6g40160 FvH4_6g40160
malus_domestica	MD09G1134700.v1.1 MD17G1123600.v1.1 MD17G1123700.v1.1
prunus_persica	Prupe.3G192000_v2.0.a1
pyrus_communis	pycom09g05580 pycom17g11510
rosa_chinensis	RchiOBHm_Chr2g0155171
rosa_laevigata	RLG00000020823
rosa_multiflora	Rmu_sc0002048.1_g000049
rosa_roxburghii	Rroxscaffold_2G00093900
rosa_rugosa	Rorug02G0445700 Rorug02G0445700
rosa_samantha	Rh2AG511900 Rh2BG522700 Rh2CG496900 Rh2DG532400
rosa_wichuraiana	Rw2G042060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1602
AccBSI	CCGCTC	1 cut(s)	1821
AciI	CCGC	1 cut(s)	1821
AcoI	YGGCCR	2 cut(s)	806, 897
AcsI	RAATTY	1 cut(s)	782
AcuI	CTGAAG	3 cut(s)	606, 687, 1233
AfaI	GTAC	3 cut(s)	556, 1211, 1769
AfiI	CCNNNNNNNGG	2 cut(s)	194, 1602
AflIII	ACRYGT	1 cut(s)	684
AgsI	TTSAA	8 cut(s)	143, 194, 319, 343, 832, 871, 1327, 1715
AjnI	CCWGG	2 cut(s)	70, 1271
AjuI	GAANNNNNNNTTGG	2 cut(s)	590, 622
AloI	GAACNNNNNNTCC	2 cut(s)	1065, 1097
Alw21I	GWGCWC	3 cut(s)	847, 999, 1389
Alw26I	GTCTC	4 cut(s)	96, 294, 791, 1834
Ama87I	CYCGRG	1 cut(s)	1294
AoxI	GGCC	2 cut(s)	806, 897
ApeKI	GCWGC	2 cut(s)	1121, 1344
ApoI	RAATTY	1 cut(s)	782
ArsI	GACNNNNNNTTYG	2 cut(s)	95, 127
AseI	ATTAAT	1 cut(s)	492
Asp700I	GAANNNNTTC	1 cut(s)	1739
AspA2I	CCTAGG	1 cut(s)	1441
AspS9I	GGNCC	2 cut(s)	1275, 1823
AsuC2I	CCSGG	3 cut(s)	1168, 1295, 1296
AsuHPI	GGTGA	8 cut(s)	236, 251, 488, 559, 572, 895, 1414, 1900
AsuII	TTCGAA	1 cut(s)	1084
AvaI	CYCGRG	1 cut(s)	1294
AvaII	GGWCC	2 cut(s)	1275, 1823
AvrII	CCTAGG	1 cut(s)	1441
AxyI	CCTNAGG	1 cut(s)	923
BaeI	ACNNNNGTAYC	2 cut(s)	1201, 1234
BalI	TGGCCA	1 cut(s)	808
BanII	GRGCYC	2 cut(s)	999, 1389
BauI	CACGAG	3 cut(s)	354, 846, 1449
BbsI	GAAGAC	2 cut(s)	164, 1722
Bbv12I	GWGCWC	3 cut(s)	847, 999, 1389
BbvI	GCAGC	2 cut(s)	1108, 1356
BccI	CCATC	9 cut(s)	92, 798, 810, 817, 999, 1070, 1251, 1370, 1793
BciT130I	CCWGG	2 cut(s)	72, 1273
BclI	TGATCA	1 cut(s)	241
BcnI	CCSGG	3 cut(s)	1168, 1295, 1296
BcoDI	GTCTC	4 cut(s)	96, 294, 791, 1834
BfaI	CTAG	2 cut(s)	1442, 1532
BfmI	CTRYAG	2 cut(s)	166, 643
BisI	GCNGC	2 cut(s)	1122, 1345
BlnI	CCTAGG	1 cut(s)	1441
BlpI	GCTNAGC	1 cut(s)	411
BlsI	GCNGC	2 cut(s)	1123, 1346
Bme1390I	CCNGG	5 cut(s)	72, 1168, 1273, 1295, 1296
Bme18I	GGWCC	2 cut(s)	1275, 1823
BmeT110I	CYCGRG	1 cut(s)	1294
BmgT120I	GGNCC	2 cut(s)	1275, 1823
BmiI	GGNNCC	5 cut(s)	1360, 1416, 1549, 1825, 1894
BmrFI	CCNGG	5 cut(s)	72, 1168, 1273, 1295, 1296
BmrI	ACTGGG	1 cut(s)	1466
BmsI	GCATC	4 cut(s)	649, 811, 1416, 1757
BmuI	ACTGGG	1 cut(s)	1466
BpiI	GAAGAC	2 cut(s)	164, 1722
BplI	GAGNNNNNCTC	2 cut(s)	983, 1015
BpmI	CTGGAG	5 cut(s)	1249, 1360, 1377, 1579, 1741
Bpu1102I	GCTNAGC	1 cut(s)	411
Bpu14I	TTCGAA	1 cut(s)	1084
BpuEI	CTTGAG	2 cut(s)	485, 1132
BpuMI	CCSGG	3 cut(s)	1168, 1295, 1296
Bsa29I	ATCGAT	1 cut(s)	1953
BsaBI	GATNNNNATC	2 cut(s)	808, 1261
BsaJI	CCNNGG	5 cut(s)	71, 1294, 1441, 1493, 1537
BsaXI	ACNNNNNCTCC	2 cut(s)	1065, 1095
Bsc4I	CCNNNNNNNGG	2 cut(s)	194, 1602
Bse118I	RCCGGY	1 cut(s)	899
Bse21I	CCTNAGG	1 cut(s)	923
Bse3DI	GCAATG	3 cut(s)	61, 541, 1278
Bse8I	GATNNNNATC	2 cut(s)	808, 1261
BseBI	CCWGG	2 cut(s)	72, 1273
BseCI	ATCGAT	1 cut(s)	1953
BseDI	CCNNGG	5 cut(s)	71, 1294, 1441, 1493, 1537
BseGI	GGATG	7 cut(s)	279, 809, 826, 1010, 1176, 1262, 1362
BseJI	GATNNNNATC	2 cut(s)	808, 1261
BseLI	CCNNNNNNNGG	2 cut(s)	194, 1602
BseMI	GCAATG	3 cut(s)	61, 541, 1278
BseMII	CTCAG	6 cut(s)	138, 204, 749, 914, 1808, 1845
BseRI	GAGGAG	1 cut(s)	1007
BseXI	GCAGC	2 cut(s)	1108, 1356
BseYI	CCCAGC	1 cut(s)	1205
BsgI	GTGCAG	2 cut(s)	1107, 1692
BshFI	GGCC	2 cut(s)	808, 899
BshVI	ATCGAT	1 cut(s)	1953
BsiHKAI	GWGCWC	3 cut(s)	847, 999, 1389
BsiHKCI	CYCGRG	1 cut(s)	1294
BsiSI	CCGG	3 cut(s)	900, 1167, 1295
BslFI	GGGAC	1 cut(s)	1258
BslI	CCNNNNNNNGG	2 cut(s)	194, 1602
BsmAI	GTCTC	4 cut(s)	96, 294, 791, 1834
BsmBI	CGTCTC	1 cut(s)	1834
BsmFI	GGGAC	1 cut(s)	1258
BsmI	GAATGC	1 cut(s)	1161
BsnI	GGCC	2 cut(s)	808, 899
BsoBI	CYCGRG	1 cut(s)	1294
Bsp119I	TTCGAA	1 cut(s)	1084
Bsp1286I	GDGCHC	3 cut(s)	847, 999, 1389
Bsp143I	GATC	6 cut(s)	241, 579, 738, 885, 1262, 1804
Bsp1720I	GCTNAGC	1 cut(s)	411
BspACI	CCGC	1 cut(s)	1821
BspANI	GGCC	2 cut(s)	808, 899
BspCNI	CTCAG	6 cut(s)	139, 205, 748, 915, 1809, 1844
BspDI	ATCGAT	1 cut(s)	1953
BspHI	TCATGA	1 cut(s)	520
BspLI	GGNNCC	5 cut(s)	1360, 1416, 1549, 1825, 1894
BspMAI	CTGCAG	1 cut(s)	647
BspQI	GCTCTTC	1 cut(s)	1574
BspT104I	TTCGAA	1 cut(s)	1084
BsrBI	CCGCTC	1 cut(s)	1821
BsrDI	GCAATG	3 cut(s)	61, 541, 1278
BsrFI	RCCGGY	1 cut(s)	899
BssAI	RCCGGY	1 cut(s)	899
BssECI	CCNNGG	5 cut(s)	71, 1294, 1441, 1493, 1537
BssMI	GATC	6 cut(s)	241, 579, 738, 885, 1262, 1804
BssSI	CACGAG	3 cut(s)	354, 846, 1449
BssT1I	CCWWGG	3 cut(s)	1441, 1493, 1537
Bst2BI	CACGAG	3 cut(s)	354, 846, 1449
Bst2UI	CCWGG	2 cut(s)	72, 1273
Bst4CI	ACNGT	6 cut(s)	473, 713, 931, 1237, 1321, 1581
Bst6I	CTCTTC	1 cut(s)	1574
BstBI	TTCGAA	1 cut(s)	1084
BstC8I	GCNNGC	4 cut(s)	396, 1143, 1285, 1884
BstDEI	CTNAG	7 cut(s)	147, 213, 411, 735, 923, 1817, 1831
BstF5I	GGATG	7 cut(s)	279, 809, 826, 1010, 1176, 1262, 1362
BstKTI	GATC	6 cut(s)	244, 582, 741, 888, 1265, 1807
BstMAI	GTCTC	4 cut(s)	96, 294, 791, 1834
BstMBI	GATC	6 cut(s)	241, 579, 738, 885, 1262, 1804
BstMWI	GCNNNNNNNGC	3 cut(s)	1211, 1350, 1745
BstNI	CCWGG	2 cut(s)	72, 1273
BstNSI	RCATGY	3 cut(s)	688, 1287, 1702
BstSCI	CCNGG	5 cut(s)	70, 1166, 1271, 1293, 1294
BstSFI	CTRYAG	2 cut(s)	166, 643
BstV1I	GCAGC	2 cut(s)	1108, 1356
BstV2I	GAAGAC	2 cut(s)	164, 1722
BstXI	CCANNNNNNTGG	2 cut(s)	440, 816
Bsu15I	ATCGAT	1 cut(s)	1953
Bsu36I	CCTNAGG	1 cut(s)	923
BsuRI	GGCC	2 cut(s)	808, 899
BsuTUI	ATCGAT	1 cut(s)	1953
BtsCI	GGATG	7 cut(s)	279, 809, 826, 1010, 1176, 1262, 1362
BtsI	GCAGTG	1 cut(s)	652
BtsIMutI	CAGTG	8 cut(s)	440, 652, 936, 1326, 1353, 1479, 1689, 1887
Cac8I	GCNNGC	4 cut(s)	396, 1143, 1285, 1884
CciI	TCATGA	1 cut(s)	520
Cfr10I	RCCGGY	1 cut(s)	899
Cfr13I	GGNCC	2 cut(s)	1275, 1823
Cfr9I	CCCGGG	1 cut(s)	1294
ClaI	ATCGAT	1 cut(s)	1953
Csp6I	GTAC	3 cut(s)	555, 1210, 1768
CviQI	GTAC	3 cut(s)	555, 1210, 1768
DdeI	CTNAG	7 cut(s)	147, 213, 411, 735, 923, 1817, 1831
DpnI	GATC	6 cut(s)	243, 581, 740, 887, 1264, 1806
DpnII	GATC	6 cut(s)	241, 579, 738, 885, 1262, 1804
EaeI	YGGCCR	2 cut(s)	806, 897
Eam1104I	CTCTTC	1 cut(s)	1574
EarI	CTCTTC	1 cut(s)	1574
Ecl136II	GAGCTC	2 cut(s)	997, 1387
Eco130I	CCWWGG	3 cut(s)	1441, 1493, 1537
Eco24I	GRGCYC	2 cut(s)	999, 1389
Eco32I	GATATC	1 cut(s)	446
Eco47I	GGWCC	2 cut(s)	1275, 1823
Eco53kI	GAGCTC	2 cut(s)	997, 1387
Eco57I	CTGAAG	3 cut(s)	606, 687, 1233
Eco81I	CCTNAGG	1 cut(s)	923
Eco88I	CYCGRG	1 cut(s)	1294
EcoICRI	GAGCTC	2 cut(s)	997, 1387
EcoRII	CCWGG	2 cut(s)	70, 1271
EcoRV	GATATC	1 cut(s)	446
EcoT14I	CCWWGG	3 cut(s)	1441, 1493, 1537
EcoT22I	ATGCAT	2 cut(s)	1064, 1750
EcoT38I	GRGCYC	2 cut(s)	999, 1389
ErhI	CCWWGG	3 cut(s)	1441, 1493, 1537
Esp3I	CGTCTC	1 cut(s)	1834
FaqI	GGGAC	1 cut(s)	1258
FauNDI	CATATG	1 cut(s)	1601
FbaI	TGATCA	1 cut(s)	241
Fnu4HI	GCNGC	2 cut(s)	1122, 1345
FokI	GGATG	7 cut(s)	286, 796, 833, 1017, 1183, 1269, 1349
FriOI	GRGCYC	2 cut(s)	999, 1389
Fsp4HI	GCNGC	2 cut(s)	1122, 1345
FspBI	CTAG	2 cut(s)	1442, 1532
GluI	GCNGC	2 cut(s)	1122, 1345
GsaI	CCCAGC	1 cut(s)	1209
GsuI	CTGGAG	5 cut(s)	1249, 1360, 1377, 1579, 1741
HaeIII	GGCC	2 cut(s)	808, 899
HapII	CCGG	3 cut(s)	900, 1167, 1295
HincII	GTYRAC	1 cut(s)	709
HindII	GTYRAC	1 cut(s)	709
HindIII	AAGCTT	2 cut(s)	677, 1052
HpaI	GTTAAC	1 cut(s)	709
HpaII	CCGG	3 cut(s)	900, 1167, 1295
HphI	GGTGA	8 cut(s)	236, 251, 488, 559, 572, 895, 1414, 1900
Hpy166II	GTNNAC	3 cut(s)	438, 709, 1927
Hpy188I	TCNGA	9 cut(s)	97, 246, 273, 586, 738, 1022, 1034, 1252, 1818
Hpy8I	GTNNAC	3 cut(s)	438, 709, 1927
HpyCH4III	ACNGT	6 cut(s)	473, 713, 931, 1237, 1321, 1581
HpyCH4IV	ACGT	1 cut(s)	1770
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1211, 1350, 1745
HpyF3I	CTNAG	7 cut(s)	147, 213, 411, 735, 923, 1817, 1831
HpySE526I	ACGT	1 cut(s)	1770
Ksp22I	TGATCA	1 cut(s)	241
KspAI	GTTAAC	1 cut(s)	709
Kzo9I	GATC	6 cut(s)	241, 579, 738, 885, 1262, 1804
LguI	GCTCTTC	1 cut(s)	1574
LmnI	GCTCC	5 cut(s)	721, 994, 1341, 1358, 1541
Lsp1109I	GCAGC	2 cut(s)	1108, 1356
LweI	GCATC	4 cut(s)	649, 811, 1416, 1757
MaeI	CTAG	2 cut(s)	1442, 1532
MaeII	ACGT	1 cut(s)	1770
MaeIII	GTNAC	4 cut(s)	27, 161, 424, 1105
MalI	GATC	6 cut(s)	243, 581, 740, 887, 1264, 1806
MbiI	CCGCTC	1 cut(s)	1821
MboI	GATC	6 cut(s)	241, 579, 738, 885, 1262, 1804
MfeI	CAATTG	1 cut(s)	1869
MhlI	GDGCHC	3 cut(s)	847, 999, 1389
MlsI	TGGCCA	1 cut(s)	808
MluCI	AATT	4 cut(s)	124, 782, 1086, 1869
MluNI	TGGCCA	1 cut(s)	808
MlyI	GAGTC	3 cut(s)	98, 296, 319
MmeI	TCCRAC	2 cut(s)	604, 1000
Mox20I	TGGCCA	1 cut(s)	808
Mph1103I	ATGCAT	2 cut(s)	1064, 1750
MroXI	GAANNNNTTC	1 cut(s)	1739
MscI	TGGCCA	1 cut(s)	808
MseI	TTAA	6 cut(s)	492, 507, 651, 708, 744, 789
MslI	CAYNNNNRTG	5 cut(s)	525, 545, 687, 814, 1067
Msp20I	TGGCCA	1 cut(s)	808
MspI	CCGG	3 cut(s)	900, 1167, 1295
MspR9I	CCNGG	5 cut(s)	72, 1168, 1273, 1295, 1296
MunI	CAATTG	1 cut(s)	1869
Mva1269I	GAATGC	1 cut(s)	1161
MvaI	CCWGG	2 cut(s)	72, 1273
MwoI	GCNNNNNNNGC	3 cut(s)	1211, 1350, 1745
NciI	CCSGG	3 cut(s)	1168, 1295, 1296
NdeI	CATATG	1 cut(s)	1601
NdeII	GATC	6 cut(s)	241, 579, 738, 885, 1262, 1804
NlaIV	GGNNCC	5 cut(s)	1360, 1416, 1549, 1825, 1894
NmuCI	GTSAC	1 cut(s)	1105
NsiI	ATGCAT	2 cut(s)	1064, 1750
NspI	RCATGY	3 cut(s)	688, 1287, 1702
NspV	TTCGAA	1 cut(s)	1084
PaeI	GCATGC	1 cut(s)	1287
PagI	TCATGA	1 cut(s)	520
PciI	ACATGT	1 cut(s)	684
PciSI	GCTCTTC	1 cut(s)	1574
PcsI	WCGNNNNNNNCGW	1 cut(s)	1767
PctI	GAATGC	1 cut(s)	1161
PdmI	GAANNNNTTC	1 cut(s)	1739
PfeI	GAWTC	7 cut(s)	217, 379, 405, 1131, 1437, 1489, 1614
PflMI	CCANNNNNTGG	1 cut(s)	1602
PkrI	GCNGC	2 cut(s)	1123, 1346
PleI	GAGTC	3 cut(s)	98, 295, 318
PpsI	GAGTC	3 cut(s)	98, 295, 318
PscI	ACATGT	1 cut(s)	684
PshBI	ATTAAT	1 cut(s)	492
Psp124BI	GAGCTC	2 cut(s)	999, 1389
Psp6I	CCWGG	2 cut(s)	70, 1271
PspFI	CCCAGC	1 cut(s)	1205
PspGI	CCWGG	2 cut(s)	70, 1271
PspN4I	GGNNCC	5 cut(s)	1360, 1416, 1549, 1825, 1894
PspPI	GGNCC	2 cut(s)	1275, 1823
PstI	CTGCAG	1 cut(s)	647
RsaI	GTAC	3 cut(s)	556, 1211, 1769
RsaNI	GTAC	3 cut(s)	555, 1210, 1768
RseI	CAYNNNNRTG	5 cut(s)	525, 545, 687, 814, 1067
SacI	GAGCTC	2 cut(s)	999, 1389
SapI	GCTCTTC	1 cut(s)	1574
SaqAI	TTAA	6 cut(s)	492, 507, 651, 708, 744, 789
SatI	GCNGC	2 cut(s)	1122, 1345
Sau3AI	GATC	6 cut(s)	241, 579, 738, 885, 1262, 1804
Sau96I	GGNCC	2 cut(s)	1275, 1823
SchI	GAGTC	3 cut(s)	98, 296, 319
ScrFI	CCNGG	5 cut(s)	72, 1168, 1273, 1295, 1296
SduI	GDGCHC	3 cut(s)	847, 999, 1389
SfaNI	GCATC	4 cut(s)	649, 811, 1416, 1757
SfcI	CTRYAG	2 cut(s)	166, 643
SfuI	TTCGAA	1 cut(s)	1084
SinI	GGWCC	2 cut(s)	1275, 1823
SmaI	CCCGGG	1 cut(s)	1296
SmiMI	CAYNNNNRTG	5 cut(s)	525, 545, 687, 814, 1067
SmlI	CTYRAG	2 cut(s)	500, 1147
SmoI	CTYRAG	2 cut(s)	500, 1147
SphI	GCATGC	1 cut(s)	1287
Sse9I	AATT	4 cut(s)	124, 782, 1086, 1869
SsiI	CCGC	1 cut(s)	1821
SspMI	CTAG	2 cut(s)	1442, 1532
SstI	GAGCTC	2 cut(s)	999, 1389
StyD4I	CCNGG	5 cut(s)	70, 1166, 1271, 1293, 1294
StyI	CCWWGG	3 cut(s)	1441, 1493, 1537
TaaI	ACNGT	6 cut(s)	473, 713, 931, 1237, 1321, 1581
TaiI	ACGT	1 cut(s)	1773
TaqI	TCGA	3 cut(s)	16, 1084, 1953
TasI	AATT	4 cut(s)	124, 782, 1086, 1869
TfiI	GAWTC	7 cut(s)	217, 379, 405, 1131, 1437, 1489, 1614
Tru1I	TTAA	6 cut(s)	492, 507, 651, 708, 744, 789
Tru9I	TTAA	6 cut(s)	492, 507, 651, 708, 744, 789
TscAI	CASTG	8 cut(s)	440, 652, 936, 1326, 1360, 1479, 1689, 1894
TseFI	GTSAC	1 cut(s)	1105
TseI	GCWGC	2 cut(s)	1121, 1344
Tsp45I	GTSAC	1 cut(s)	1105
TspDTI	ATGAA	9 cut(s)	509, 543, 768, 789, 842, 1001, 1123, 1561, 1576
TspGWI	ACGGA	1 cut(s)	1525
TspMI	CCCGGG	1 cut(s)	1294
TspRI	CASTG	8 cut(s)	440, 652, 936, 1326, 1360, 1479, 1689, 1894
Van91I	CCANNNNNTGG	1 cut(s)	1602
VpaK11BI	GGWCC	2 cut(s)	1275, 1823
VspI	ATTAAT	1 cut(s)	492
XapI	RAATTY	1 cut(s)	782
XceI	RCATGY	3 cut(s)	688, 1287, 1702
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1792
XmaI	CCCGGG	1 cut(s)	1294
XmaJI	CCTAGG	1 cut(s)	1441
XmnI	GAANNNNTTC	1 cut(s)	1739
XspI	CTAG	2 cut(s)	1442, 1532
Zsp2I	ATGCAT	2 cut(s)	1064, 1750

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.