Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0008984

Physical Location & Seq

Genomic Coordinates Reverse (-)

10755189 .. 10757621

Gene Structure 2433 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1123700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1995 bp

ATGGCGATATCGAATCCGGGAGTGCCCTCAGTCAAATGGGCTCTGCTTGTTTTGGTTGTATTTGTGCACACCTGTAATGGGTTTTATCTCCCCGGAAGCTACATGCATAGATATTCGACAGGGCAAGAAATCATTACCGAAGTTAACTCCTTGACTTCTATAGGCACTGGGCTGCCCTTCAGCTACTACAGTCTCCCTTATTGCAAACCCCCTGGAGGTATCAAGAAAAGTGCTGAGAATCTCGGGGAACTCCTTATGGGAGATGAGATCGAAACCTCTGCGTACCTCTTCAAAATGAATATGAATGAGACTCTTTACGTGTGCACCACACCCCCCTTGACTAAGCACGACGTAAAGCTGCTGAAACAGAGGACCCGTGATCTGTATCAGGTCAATATGATCCTTGATAATTTGCCTGCGATGAGGTATGCTTATCAAAATGGGGTTAAGATTCAGTGGACTGGGTTTCCTGTTGGGTACACACAACCCAACGGTAAAGAAGATTACATCATCAATCACCTCAAGTTCACTGTTTTGATTCATGAGTATGAAGGGAGTGGTGTGCAGATAATCGGGACTGGGGAAGAAGGCATGGGTGTCATTTCAGAAGCTGATAAGAAGAAGGCATCTGGGTTTGAGATTGTTGGCTTTGAGGTTAAACCTTACAGCATTAAATATGACCCAGAGGCCATGAAGAAAAAAGCCATGTATGAAAAAATCCAACCTGTCAATTTTCCCACGGTGGTCGAAAAGTCTCAAATAATAAGGGAGCAAGAGAAAGTATCATTCACTTATGAGGTTGAGTTTGTGAAAAGCGATATAAGATGGCCATCAAGGTGGGATGCTTATTTGAAGATGGAGGGTGCTCGTGTGCACTGGTTCTCTATCCTGAATTCATTAATGGTGATCCTTTTCCTCGCTGGTATAGTCTTTGTCATATTCCTAAGGACTGCGAGGAGGGATTTAACAAGATACGAACAACTGGACGAAGAACCTCAAGCTCAGATGAATGAAGAGCTCTATGGGTGGAAGCTTGTTGTGGGAGATGTGTTCAGAGAACCAGATTCTCCCAAGCTTCTTTGCGTGATGGTTGGAGATGGGGTTCAGATTACAGGGATGGCAGTTGTCACAATCATCTTTGCAGCATTTGGTTTCATGTCACCAGCTTCACGAGGAATGCTACTGACAGGAATGATCATTCTATATCTTTTCCTTGGGATTATTGCTGGTTATGTTGCTGTCCGTATGTGGATAACCATAAAGGGAACTTCCGAAGGATGGAAATCGGTTTCCTGGTCTGTGGCGTGCTTCTTTCCTGGAATCGTCATTGTTATTCGTACAATACTGAATTTCATTCTATGGGGCAGCAAGAGTACTGGTGCTCTTCCCATTTCTTTGTATTTTGTACTCATCTCCCTCTGGTTCTGCATTTCCGTGCCTCTCACCCTTCTCGGGGGATTCTTAGGCACACGAGCTGAGCCAATTTCATATCCAGTAAGAACCAACCAGATTCCAAGGGAGATTCCTGCGCGCAAATATCCATCATGGCTTCTTGTTCTTGGTGCTGGGACACTTCCGTTTGGAACCCTTTTCATTGAACTCTTCTTCATCCTTTCTAGCATCTGGCTTGGAAGATTTTATTATGTCTTCGGTTTCCTTTTTATAGTGCTTTTGTTGTTGGTTATCGTTTGTGCTGAAGTTTCAGTAGTTATCACATACATGCATCTTTGTGTGGGGGATTGGAGGTGGTGGTGGAAGGCTTTCTTTGCCTCAGGTTCAGCTTCCTTTTACGTGTTCCTGTACTCCATCAATTACCTGGTATTTGACCTGCAGGGTTTGGGTGGGCTTGCATCGGTTATCCTTTACCTCGGTTATTCATTGATCATGGCAACTGCAATCATGCTGTCAACTGGCACCATTGGCTTTCTCATGTCTTTCTACTTTGTTCGTTACCTCTGCTCATCCGTAAGGATCAACTATAAAAGTGTTGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0005911 GO:0008150 GO:0009506 GO:0012505 GO:0016020 GO:0016021 GO:0030054 GO:0031090 GO:0031224 GO:0031410 GO:0031982 GO:0031984 GO:0032386 GO:0032388 GO:0032879 GO:0032880 GO:0033157 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048518 GO:0050789 GO:0051049 GO:0051050 GO:0051222 GO:0051223 GO:0055044 GO:0060341 GO:0065007 GO:0070201 GO:0070861 GO:0070863 GO:0090087 GO:0090316 GO:0097708 GO:0098588 GO:0098791 GO:0098805 GO:1903827 GO:1903829 GO:1904951 GO:2000008 GO:2000010

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EMP70

Protein Analysis

665

Amino Acids

75.0

Weight (kDa)

8.58

Isoelectric Point (pI)

36.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EMP70	PF02990	59 - 611	4.7e-178	Endomembrane protein 70

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008984)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G12650
fragaria_vesca	FvH4_6g40160 FvH4_6g40160 FvH4_6g40160 FvH4_6g40160
malus_domestica	MD09G1134700.v1.1 MD17G1123600.v1.1 MD17G1123700.v1.1
prunus_persica	Prupe.3G192000_v2.0.a1
pyrus_communis	pycom09g05580 pycom17g11510
rosa_chinensis	RchiOBHm_Chr2g0155171
rosa_laevigata	RLG00000020823
rosa_multiflora	Rmu_sc0002048.1_g000049
rosa_roxburghii	Rroxscaffold_2G00093900
rosa_rugosa	Rorug02G0445700 Rorug02G0445700
rosa_samantha	Rh2AG511900 Rh2BG522700 Rh2CG496900 Rh2DG532400
rosa_wichuraiana	Rw2G042060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1836
AccB1I	GGYRCC	1 cut(s)	1913
AccII	CGCG	1 cut(s)	1531
AclWI	GGATC	3 cut(s)	394, 901, 1979
AcoI	YGGCCR	1 cut(s)	827
AcsI	RAATTY	2 cut(s)	892, 1348
AcuI	CTGAAG	2 cut(s)	163, 1716
AfaI	GTAC	6 cut(s)	284, 479, 1339, 1375, 1407, 1802
AfiI	CCNNNNNNNGG	5 cut(s)	78, 215, 1278, 1452, 1453
AflIII	ACRYGT	2 cut(s)	318, 1791
AgsI	TTSAA	3 cut(s)	292, 853, 1598
AjnI	CCWGG	4 cut(s)	211, 1292, 1315, 1815
AloI	GAACNNNNNNTCC	2 cut(s)	1086, 1118
Alw21I	GWGCWC	6 cut(s)	69, 326, 868, 876, 1020, 1384
Alw26I	GTCTC	3 cut(s)	197, 302, 759
Alw44I	GTGCAC	3 cut(s)	65, 322, 872
AlwI	GGATC	3 cut(s)	394, 901, 1979
AlwNI	CAGNNNCTG	1 cut(s)	611
Ama87I	CYCGRG	2 cut(s)	242, 1451
AoxI	GGCC	2 cut(s)	687, 827
ApaLI	GTGCAC	3 cut(s)	65, 322, 872
ApeKI	GCWGC	4 cut(s)	172, 358, 1142, 1365
ApoI	RAATTY	2 cut(s)	892, 1348
AseI	ATTAAT	1 cut(s)	899
Asp700I	GAANNNNTTC	1 cut(s)	1760
AspLEI	GCGC	2 cut(s)	1531, 1533
AspS9I	GGNCC	1 cut(s)	372
AsuC2I	CCSGG	2 cut(s)	18, 93
AsuHPI	GGTGA	4 cut(s)	509, 916, 1152, 1435
AvaI	CYCGRG	2 cut(s)	242, 1451
AvaII	GGWCC	1 cut(s)	372
AxyI	CCTNAGG	2 cut(s)	944, 1771
BaeGI	GKGCMC	4 cut(s)	27, 69, 326, 876
BalI	TGGCCA	1 cut(s)	829
BanI	GGYRCC	1 cut(s)	1913
BanII	GRGCYC	2 cut(s)	43, 1020
BauI	CACGAG	3 cut(s)	867, 1170, 1470
BbsI	GAAGAC	1 cut(s)	1639
Bbv12I	GWGCWC	6 cut(s)	69, 326, 868, 876, 1020, 1384
BbvI	GCAGC	4 cut(s)	159, 345, 1154, 1377
BccI	CCATC	9 cut(s)	819, 838, 850, 1081, 1091, 1111, 1272, 1549, 1814
BcgI	CGANNNNNNTGC	2 cut(s)	260, 294
BciT130I	CCWGG	4 cut(s)	213, 1294, 1317, 1817
BclI	TGATCA	2 cut(s)	1194, 1881
BcnI	CCSGG	2 cut(s)	18, 93
BcoDI	GTCTC	3 cut(s)	197, 302, 759
BfaI	CTAG	1 cut(s)	1617
BfmI	CTRYAG	3 cut(s)	159, 187, 1829
BfuAI	ACCTGC	1 cut(s)	1836
BisI	GCNGC	4 cut(s)	173, 359, 1143, 1366
BlpI	GCTNAGC	1 cut(s)	1476
BlsI	GCNGC	4 cut(s)	174, 360, 1144, 1367
BmcAI	AGTACT	1 cut(s)	1375
Bme1390I	CCNGG	6 cut(s)	18, 93, 213, 1294, 1317, 1817
Bme18I	GGWCC	1 cut(s)	372
BmeT110I	CYCGRG	2 cut(s)	242, 1451
BmgT120I	GGNCC	1 cut(s)	372
BmiI	GGNNCC	3 cut(s)	374, 1585, 1915
BmrFI	CCNGG	6 cut(s)	18, 93, 213, 1294, 1317, 1817
BmrI	ACTGGG	3 cut(s)	177, 471, 588
BmsI	GCATC	5 cut(s)	635, 832, 1629, 1732, 1859
BmuI	ACTGGG	3 cut(s)	177, 471, 588
BpiI	GAAGAC	1 cut(s)	1639
BpmI	CTGGAG	1 cut(s)	234
Bpu1102I	GCTNAGC	1 cut(s)	1476
BpuEI	CTTGAG	2 cut(s)	506, 981
BpuMI	CCSGG	2 cut(s)	18, 93
BsaAI	YACGTR	2 cut(s)	319, 1792
BsaBI	GATNNNNATC	3 cut(s)	384, 829, 1282
BsaJI	CCNNGG	6 cut(s)	91, 211, 738, 1213, 1514, 1867
BsaXI	ACNNNNNCTCC	2 cut(s)	1086, 1116
Bsc4I	CCNNNNNNNGG	5 cut(s)	78, 215, 1278, 1452, 1453
Bse1I	ACTGG	8 cut(s)	172, 466, 583, 881, 987, 1381, 1493, 1915
Bse21I	CCTNAGG	2 cut(s)	944, 1771
Bse8I	GATNNNNATC	3 cut(s)	384, 829, 1282
BseBI	CCWGG	4 cut(s)	213, 1294, 1317, 1817
BseDI	CCNNGG	6 cut(s)	91, 211, 738, 1213, 1514, 1867
BseGI	GGATG	5 cut(s)	847, 1122, 1283, 1608, 1961
BseJI	GATNNNNATC	3 cut(s)	384, 829, 1282
BseLI	CCNNNNNNNGG	5 cut(s)	78, 215, 1278, 1452, 1453
BseMII	CTCAG	5 cut(s)	42, 225, 1016, 1467, 1785
BseNI	ACTGG	8 cut(s)	172, 466, 583, 881, 987, 1381, 1493, 1915
BsePI	GCGCGC	1 cut(s)	1529
BseRI	GAGGAG	1 cut(s)	970
BseSI	GKGCMC	4 cut(s)	27, 69, 326, 876
BseXI	GCAGC	4 cut(s)	159, 345, 1154, 1377
BseYI	CCCAGC	1 cut(s)	1565
BsgI	GTGCAG	1 cut(s)	584
Bsh1236I	CGCG	1 cut(s)	1531
BshFI	GGCC	2 cut(s)	689, 829
BshNI	GGYRCC	1 cut(s)	1913
BsiHKAI	GWGCWC	6 cut(s)	69, 326, 868, 876, 1020, 1384
BsiHKCI	CYCGRG	2 cut(s)	242, 1451
BsiSI	CCGG	2 cut(s)	17, 93
BslFI	GGGAC	2 cut(s)	589, 1582
BslI	CCNNNNNNNGG	5 cut(s)	78, 215, 1278, 1452, 1453
BsmAI	GTCTC	3 cut(s)	197, 302, 759
BsmFI	GGGAC	2 cut(s)	589, 1582
BsmI	GAATGC	1 cut(s)	1182
BsnI	GGCC	2 cut(s)	689, 829
BsoBI	CYCGRG	2 cut(s)	242, 1451
Bsp1286I	GDGCHC	8 cut(s)	27, 43, 69, 326, 868, 876, 1020, 1384
Bsp143I	GATC	7 cut(s)	267, 379, 399, 906, 1194, 1881, 1971
Bsp1720I	GCTNAGC	1 cut(s)	1476
BspANI	GGCC	2 cut(s)	689, 829
BspCNI	CTCAG	5 cut(s)	41, 226, 1015, 1468, 1784
BspFNI	CGCG	1 cut(s)	1531
BspHI	TCATGA	1 cut(s)	541
BspLI	GGNNCC	3 cut(s)	374, 1585, 1915
BspMAI	CTGCAG	1 cut(s)	1833
BspMI	ACCTGC	1 cut(s)	1836
BspPI	GGATC	3 cut(s)	394, 901, 1979
BspQI	GCTCTTC	2 cut(s)	1008, 1389
BspT107I	GGYRCC	1 cut(s)	1913
BsrI	ACTGG	8 cut(s)	172, 466, 583, 881, 987, 1381, 1493, 1915
BssECI	CCNNGG	6 cut(s)	91, 211, 738, 1213, 1514, 1867
BssHII	GCGCGC	1 cut(s)	1529
BssMI	GATC	7 cut(s)	267, 379, 399, 906, 1194, 1881, 1971
BssSI	CACGAG	3 cut(s)	867, 1170, 1470
BssT1I	CCWWGG	2 cut(s)	1213, 1514
Bst2BI	CACGAG	3 cut(s)	867, 1170, 1470
Bst2UI	CCWGG	4 cut(s)	213, 1294, 1317, 1817
Bst4CI	ACNGT	4 cut(s)	191, 494, 532, 742
Bst6I	CTCTTC	4 cut(s)	293, 1008, 1389, 1607
BstBAI	YACGTR	2 cut(s)	319, 1792
BstC8I	GCNNGC	4 cut(s)	417, 1306, 1531, 1848
BstDEI	CTNAG	8 cut(s)	28, 234, 342, 944, 1002, 1462, 1476, 1771
BstDSI	CCRYGG	1 cut(s)	738
BstF5I	GGATG	5 cut(s)	847, 1122, 1283, 1608, 1961
BstFNI	CGCG	1 cut(s)	1531
BstHHI	GCGC	2 cut(s)	1531, 1533
BstKTI	GATC	7 cut(s)	270, 382, 402, 909, 1197, 1884, 1974
BstMAI	GTCTC	3 cut(s)	197, 302, 759
BstMBI	GATC	7 cut(s)	267, 379, 399, 906, 1194, 1881, 1971
BstMWI	GCNNNNNNNGC	2 cut(s)	1766, 1920
BstNI	CCWGG	4 cut(s)	213, 1294, 1317, 1817
BstNSI	RCATGY	2 cut(s)	106, 1723
BstSCI	CCNGG	6 cut(s)	16, 91, 211, 1292, 1315, 1815
BstSFI	CTRYAG	3 cut(s)	159, 187, 1829
BstSLI	GKGCMC	4 cut(s)	27, 69, 326, 876
BstUI	CGCG	1 cut(s)	1531
BstV1I	GCAGC	4 cut(s)	159, 345, 1154, 1377
BstV2I	GAAGAC	1 cut(s)	1639
BstXI	CCANNNNNNTGG	1 cut(s)	837
Bsu36I	CCTNAGG	2 cut(s)	944, 1771
BsuRI	GGCC	2 cut(s)	689, 829
BtgI	CCRYGG	1 cut(s)	738
BtgZI	GCGATG	1 cut(s)	434
BtsCI	GGATG	5 cut(s)	847, 1122, 1283, 1608, 1961
BtsIMutI	CAGTG	4 cut(s)	165, 461, 528, 874
BveI	ACCTGC	1 cut(s)	1836
Cac8I	GCNNGC	4 cut(s)	417, 1306, 1531, 1848
CaiI	CAGNNNCTG	1 cut(s)	611
CciI	TCATGA	1 cut(s)	541
CfoI	GCGC	2 cut(s)	1531, 1533
Cfr13I	GGNCC	1 cut(s)	372
CsiI	ACCWGGT	1 cut(s)	1815
Csp6I	GTAC	6 cut(s)	283, 478, 1338, 1374, 1406, 1801
CviQI	GTAC	6 cut(s)	283, 478, 1338, 1374, 1406, 1801
DdeI	CTNAG	8 cut(s)	28, 234, 342, 944, 1002, 1462, 1476, 1771
DpnI	GATC	7 cut(s)	269, 381, 401, 908, 1196, 1883, 1973
DpnII	GATC	7 cut(s)	267, 379, 399, 906, 1194, 1881, 1971
EaeI	YGGCCR	1 cut(s)	827
Eam1104I	CTCTTC	4 cut(s)	293, 1008, 1389, 1607
EarI	CTCTTC	4 cut(s)	293, 1008, 1389, 1607
Ecl136II	GAGCTC	1 cut(s)	1018
Eco130I	CCWWGG	2 cut(s)	1213, 1514
Eco24I	GRGCYC	2 cut(s)	43, 1020
Eco32I	GATATC	1 cut(s)	9
Eco47I	GGWCC	1 cut(s)	372
Eco53kI	GAGCTC	1 cut(s)	1018
Eco57I	CTGAAG	2 cut(s)	163, 1716
Eco81I	CCTNAGG	2 cut(s)	944, 1771
Eco88I	CYCGRG	2 cut(s)	242, 1451
EcoICRI	GAGCTC	1 cut(s)	1018
EcoO109I	RGGNCCY	1 cut(s)	372
EcoRI	GAATTC	1 cut(s)	892
EcoRII	CCWGG	4 cut(s)	211, 1292, 1315, 1815
EcoRV	GATATC	1 cut(s)	9
EcoT14I	CCWWGG	2 cut(s)	1213, 1514
EcoT22I	ATGCAT	2 cut(s)	108, 1725
EcoT38I	GRGCYC	2 cut(s)	43, 1020
ErhI	CCWWGG	2 cut(s)	1213, 1514
FalI	AAGNNNNNCTT	2 cut(s)	1748, 1780
FaqI	GGGAC	2 cut(s)	589, 1582
FbaI	TGATCA	2 cut(s)	1194, 1881
Fnu4HI	GCNGC	4 cut(s)	173, 359, 1143, 1366
FokI	GGATG	5 cut(s)	854, 1129, 1290, 1595, 1948
FriOI	GRGCYC	2 cut(s)	43, 1020
Fsp4HI	GCNGC	4 cut(s)	173, 359, 1143, 1366
FspBI	CTAG	1 cut(s)	1617
GlaI	GCGC	2 cut(s)	1530, 1532
GluI	GCNGC	4 cut(s)	173, 359, 1143, 1366
GsaI	CCCAGC	1 cut(s)	1569
GsuI	CTGGAG	1 cut(s)	234
HaeIII	GGCC	2 cut(s)	689, 829
HapII	CCGG	2 cut(s)	17, 93
HhaI	GCGC	2 cut(s)	1531, 1533
Hin6I	GCGC	2 cut(s)	1529, 1531
HinP1I	GCGC	2 cut(s)	1529, 1531
HincII	GTYRAC	2 cut(s)	145, 1908
HindII	GTYRAC	2 cut(s)	145, 1908
HindIII	AAGCTT	2 cut(s)	1031, 1073
HpaI	GTTAAC	1 cut(s)	145
HpaII	CCGG	2 cut(s)	17, 93
HphI	GGTGA	4 cut(s)	509, 916, 1152, 1435
Hpy166II	GTNNAC	8 cut(s)	67, 145, 324, 459, 480, 528, 874, 1908
Hpy188I	TCNGA	5 cut(s)	607, 1005, 1055, 1107, 1273
Hpy188III	TCNNGA	5 cut(s)	223, 542, 574, 889, 1170
Hpy8I	GTNNAC	8 cut(s)	67, 145, 324, 459, 480, 528, 874, 1908
Hpy99I	CGWCG	1 cut(s)	353
HpyAV	CCTTC	7 cut(s)	187, 545, 581, 616, 1268, 1457, 1750
HpyCH4III	ACNGT	4 cut(s)	191, 494, 532, 742
HpyCH4IV	ACGT	3 cut(s)	318, 351, 1791
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1766, 1920
HpyF3I	CTNAG	8 cut(s)	28, 234, 342, 944, 1002, 1462, 1476, 1771
HpySE526I	ACGT	3 cut(s)	318, 351, 1791
HspAI	GCGC	2 cut(s)	1529, 1531
Ksp22I	TGATCA	2 cut(s)	1194, 1881
KspAI	GTTAAC	1 cut(s)	145
Kzo9I	GATC	7 cut(s)	267, 379, 399, 906, 1194, 1881, 1971
LguI	GCTCTTC	2 cut(s)	1008, 1389
LmnI	GCTCC	1 cut(s)	769
Lsp1109I	GCAGC	4 cut(s)	159, 345, 1154, 1377
LweI	GCATC	5 cut(s)	635, 832, 1629, 1732, 1859
MabI	ACCWGGT	1 cut(s)	1815
MaeI	CTAG	1 cut(s)	1617
MaeII	ACGT	3 cut(s)	318, 351, 1791
MaeIII	GTNAC	3 cut(s)	1126, 1158, 1949
MalI	GATC	7 cut(s)	269, 381, 401, 908, 1196, 1883, 1973
MboI	GATC	7 cut(s)	267, 379, 399, 906, 1194, 1881, 1971
MhlI	GDGCHC	8 cut(s)	27, 43, 69, 326, 868, 876, 1020, 1384
MlsI	TGGCCA	1 cut(s)	829
MluCI	AATT	6 cut(s)	409, 730, 892, 1348, 1482, 1810
MluNI	TGGCCA	1 cut(s)	829
MlyI	GAGTC	1 cut(s)	304
MmeI	TCCRAC	2 cut(s)	745, 1072
Mox20I	TGGCCA	1 cut(s)	829
Mph1103I	ATGCAT	2 cut(s)	108, 1725
MroXI	GAANNNNTTC	1 cut(s)	1760
MscI	TGGCCA	1 cut(s)	829
MseI	TTAA	6 cut(s)	144, 447, 657, 672, 899, 965
MslI	CAYNNNNRTG	5 cut(s)	546, 835, 1433, 1718, 1728
Msp20I	TGGCCA	1 cut(s)	829
MspI	CCGG	2 cut(s)	17, 93
MspR9I	CCNGG	6 cut(s)	18, 93, 213, 1294, 1317, 1817
Mva1269I	GAATGC	1 cut(s)	1182
MvaI	CCWGG	4 cut(s)	213, 1294, 1317, 1817
MvnI	CGCG	1 cut(s)	1531
MwoI	GCNNNNNNNGC	2 cut(s)	1766, 1920
NciI	CCSGG	2 cut(s)	18, 93
NdeII	GATC	7 cut(s)	267, 379, 399, 906, 1194, 1881, 1971
NlaIV	GGNNCC	3 cut(s)	374, 1585, 1915
NmuCI	GTSAC	2 cut(s)	1126, 1158
NsiI	ATGCAT	2 cut(s)	108, 1725
NspI	RCATGY	2 cut(s)	106, 1723
PagI	TCATGA	1 cut(s)	541
PauI	GCGCGC	1 cut(s)	1529
PciSI	GCTCTTC	2 cut(s)	1008, 1389
PctI	GAATGC	1 cut(s)	1182
PdmI	GAANNNNTTC	1 cut(s)	1760
PfeI	GAWTC	9 cut(s)	13, 238, 451, 538, 1064, 1320, 1458, 1510, 1522
PfoI	TCCNGGA	2 cut(s)	16, 1315
PkrI	GCNGC	4 cut(s)	174, 360, 1144, 1367
PleI	GAGTC	1 cut(s)	304
PpsI	GAGTC	1 cut(s)	304
Ppu21I	YACGTR	2 cut(s)	319, 1792
PpuMI	RGGWCCY	1 cut(s)	372
PshBI	ATTAAT	1 cut(s)	899
Psp124BI	GAGCTC	1 cut(s)	1020
Psp5II	RGGWCCY	1 cut(s)	372
Psp6I	CCWGG	4 cut(s)	211, 1292, 1315, 1815
PspFI	CCCAGC	1 cut(s)	1565
PspGI	CCWGG	4 cut(s)	211, 1292, 1315, 1815
PspN4I	GGNNCC	3 cut(s)	374, 1585, 1915
PspPI	GGNCC	1 cut(s)	372
PspPPI	RGGWCCY	1 cut(s)	372
PstI	CTGCAG	1 cut(s)	1833
PstNI	CAGNNNCTG	1 cut(s)	611
PteI	GCGCGC	1 cut(s)	1529
RsaI	GTAC	6 cut(s)	284, 479, 1339, 1375, 1407, 1802
RsaNI	GTAC	6 cut(s)	283, 478, 1338, 1374, 1406, 1801
RseI	CAYNNNNRTG	5 cut(s)	546, 835, 1433, 1718, 1728
SacI	GAGCTC	1 cut(s)	1020
SapI	GCTCTTC	2 cut(s)	1008, 1389
SaqAI	TTAA	6 cut(s)	144, 447, 657, 672, 899, 965
SatI	GCNGC	4 cut(s)	173, 359, 1143, 1366
Sau3AI	GATC	7 cut(s)	267, 379, 399, 906, 1194, 1881, 1971
Sau96I	GGNCC	1 cut(s)	372
SbfI	CCTGCAGG	1 cut(s)	1833
ScaI	AGTACT	1 cut(s)	1375
SchI	GAGTC	1 cut(s)	304
ScrFI	CCNGG	6 cut(s)	18, 93, 213, 1294, 1317, 1817
SdaI	CCTGCAGG	1 cut(s)	1833
SduI	GDGCHC	8 cut(s)	27, 43, 69, 326, 868, 876, 1020, 1384
SexAI	ACCWGGT	1 cut(s)	1815
SfaNI	GCATC	5 cut(s)	635, 832, 1629, 1732, 1859
SfcI	CTRYAG	3 cut(s)	159, 187, 1829
SinI	GGWCC	1 cut(s)	372
SmiMI	CAYNNNNRTG	5 cut(s)	546, 835, 1433, 1718, 1728
SmlI	CTYRAG	2 cut(s)	521, 996
SmoI	CTYRAG	2 cut(s)	521, 996
Sse8387I	CCTGCAGG	1 cut(s)	1833
Sse9I	AATT	6 cut(s)	409, 730, 892, 1348, 1482, 1810
SspMI	CTAG	1 cut(s)	1617
SstI	GAGCTC	1 cut(s)	1020
StyD4I	CCNGG	6 cut(s)	16, 91, 211, 1292, 1315, 1815
StyI	CCWWGG	2 cut(s)	1213, 1514
TaaI	ACNGT	4 cut(s)	191, 494, 532, 742
TaiI	ACGT	3 cut(s)	321, 354, 1794
TaqI	TCGA	4 cut(s)	11, 116, 270, 747
TasI	AATT	6 cut(s)	409, 730, 892, 1348, 1482, 1810
TatI	WGTACW	3 cut(s)	1373, 1405, 1800
TfiI	GAWTC	9 cut(s)	13, 238, 451, 538, 1064, 1320, 1458, 1510, 1522
Tru1I	TTAA	6 cut(s)	144, 447, 657, 672, 899, 965
Tru9I	TTAA	6 cut(s)	144, 447, 657, 672, 899, 965
TscAI	CASTG	4 cut(s)	172, 461, 535, 881
TseFI	GTSAC	2 cut(s)	1126, 1158
TseI	GCWGC	4 cut(s)	172, 358, 1142, 1365
Tsp45I	GTSAC	2 cut(s)	1126, 1158
TspGWI	ACGGA	4 cut(s)	1232, 1423, 1566, 1954
TspRI	CASTG	4 cut(s)	172, 461, 535, 881
VneI	GTGCAC	3 cut(s)	65, 322, 872
VpaK11BI	GGWCC	1 cut(s)	372
VspI	ATTAAT	1 cut(s)	899
XapI	RAATTY	2 cut(s)	892, 1348
XceI	RCATGY	2 cut(s)	106, 1723
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1813
XmnI	GAANNNNTTC	1 cut(s)	1760
XspI	CTAG	1 cut(s)	1617
ZrmI	AGTACT	1 cut(s)	1375
Zsp2I	ATGCAT	2 cut(s)	108, 1725

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.