AT4G14305

Peroxisomal membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0017089
Physical Location & Seq
Reverse (-)
8235093 .. 8236744
1652 bp
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UTR
Exon/CDS
Intron
AT4G14305.2

Sequence Viewer

Length: 558 bp
ATGTCGGATCTTGCTAAAGACGCATGGCGAAAGTATCTGATTCAGCTTCAAGCTCATCCTTTACGAACCAAGGCGATCACTGCAGGGGTTTTAGCTGGATGTAGTGATGCTATAGCTCAGAAGATCTCTGGAGTTAAACGGATTCAGTTCCGGAGATTGCTTCTCCTTATGTTGTATGGCTTTGCTTATGGAGGACCATTTGGTCACTTTTTCCACAAATTGATGGATACCATTTTCAAAGGGAAGAAGGGTAATTCAACTGTTGCCAAAAAGGTTTTGTTGGAGCAACTTACATCATCTCCTTGGAACAATTTTCTTTTCATGTCTTACTATGGTTTAGTAGTTGAAGGAAGGCCATGGAAGCTAGTGAAGCACAAACTTGGAAAAGATTACCCTACTATCCAGTTAACCGCATGGAAGTTCTGGCCTATCGTGGGATGGGTTAATTATCAGTATGTGCCTCTCCAATTCCGTGTTTTGTTCAGCAGCTTCGTCGCCTCTTGCTGGAGCATATTTCTGAACTTGAAAGCGCGATCTCCGGTGATCAAAAACGCGTAG

Protein Analysis

185

Amino Acids

21.21

Weight (kDa)

10.2

Isoelectric Point (pI)

33.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpv17_PMP22 PF04117 102 - 162 1.9e-20 Mpv17 / PMP22 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017089)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14305
fragaria_vesca FvH4_4g04080
malus_domestica MD13G1217400.v1.1
prunus_persica Prupe.1G044000_v2.0.a1
pyrus_communis pycom13g19080
rosa_chinensis RchiOBHm_Chr4g0394471
rosa_laevigata RLG00000009696
rosa_multiflora Rmu_sc0001582.1_g000001
rosa_roxburghii Rroxscaffold_5G00339490
rosa_rugosa Rorug03G0365900.1
rosa_samantha Rh4AG052200
rosa_wichuraiana Rw0G003390 Rw4G004210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 201
AccII CGCG 2 cut(s) 532, 554
AccIII TCCGGA 1 cut(s) 150
AciI CCGC 1 cut(s) 411
AclWI GGATC 1 cut(s) 15
AfiI CCNNNNNNNGG 2 cut(s) 434, 504
AflIII ACRYGT 1 cut(s) 552
AgsI TTSAA 5 cut(s) 50, 238, 258, 347, 526
AluBI AGCT 6 cut(s) 46, 53, 95, 116, 364, 489
AluI AGCT 6 cut(s) 46, 53, 95, 116, 364, 489
AlwI GGATC 1 cut(s) 15
Aor13HI TCCGGA 1 cut(s) 150
AoxI GGCC 2 cut(s) 353, 425
ApeKI GCWGC 1 cut(s) 486
AspLEI GCGC 1 cut(s) 532
AspS9I GGNCC 1 cut(s) 194
AsuHPI GGTGA 1 cut(s) 553
AvaII GGWCC 1 cut(s) 194
BbvI GCAGC 1 cut(s) 498
BccI CCATC 2 cut(s) 217, 432
BcgI CGANNNNNNTGC 2 cut(s) 475, 509
BciVI GTATCC 1 cut(s) 220
BclI TGATCA 1 cut(s) 543
BfaI CTAG 1 cut(s) 365
BfmI CTRYAG 2 cut(s) 81, 111
BfuI GTATCC 1 cut(s) 220
BglII AGATCT 1 cut(s) 123
BisI GCNGC 1 cut(s) 487
BlsI GCNGC 1 cut(s) 488
Bme18I GGWCC 1 cut(s) 194
BmgT120I GGNCC 1 cut(s) 194
BmsI GCATC 1 cut(s) 97
BpmI CTGGAG 2 cut(s) 150, 526
BsaJI CCNNGG 3 cut(s) 69, 302, 356
BsaWI WCCGGW 2 cut(s) 150, 538
BsaXI ACNNNNNCTCC 2 cut(s) 283, 313
Bsc4I CCNNNNNNNGG 2 cut(s) 434, 504
Bse1I ACTGG 1 cut(s) 403
BseAI TCCGGA 1 cut(s) 150
BseDI CCNNGG 3 cut(s) 69, 302, 356
BseGI GGATG 3 cut(s) 55, 104, 443
BseLI CCNNNNNNNGG 2 cut(s) 434, 504
BseMII CTCAG 1 cut(s) 131
BseNI ACTGG 1 cut(s) 403
BseXI GCAGC 1 cut(s) 498
Bsh1236I CGCG 2 cut(s) 532, 554
BshFI GGCC 2 cut(s) 355, 427
BsiSI CCGG 2 cut(s) 151, 539
BslI CCNNNNNNNGG 2 cut(s) 434, 504
BsnI GGCC 2 cut(s) 355, 427
Bsp13I TCCGGA 1 cut(s) 150
Bsp143I GATC 5 cut(s) 7, 75, 123, 533, 543
Bsp19I CCATGG 1 cut(s) 356
BspACI CCGC 1 cut(s) 411
BspANI GGCC 2 cut(s) 355, 427
BspCNI CTCAG 1 cut(s) 130
BspEI TCCGGA 1 cut(s) 150
BspFNI CGCG 2 cut(s) 532, 554
BspMAI CTGCAG 1 cut(s) 85
BspPI GGATC 1 cut(s) 15
BsrI ACTGG 1 cut(s) 403
BssECI CCNNGG 3 cut(s) 69, 302, 356
BssMI GATC 5 cut(s) 7, 75, 123, 533, 543
BssT1I CCWWGG 3 cut(s) 69, 302, 356
Bst4CI ACNGT 1 cut(s) 262
BstDEI CTNAG 1 cut(s) 117
BstDSI CCRYGG 1 cut(s) 356
BstF5I GGATG 3 cut(s) 55, 104, 443
BstFNI CGCG 2 cut(s) 532, 554
BstHHI GCGC 1 cut(s) 532
BstKTI GATC 5 cut(s) 10, 78, 126, 536, 546
BstMBI GATC 5 cut(s) 7, 75, 123, 533, 543
BstMWI GCNNNNNNNGC 4 cut(s) 20, 80, 361, 370
BstSFI CTRYAG 2 cut(s) 81, 111
BstUI CGCG 2 cut(s) 532, 554
BstV1I GCAGC 1 cut(s) 498
BstX2I RGATCY 2 cut(s) 7, 123
BstYI RGATCY 2 cut(s) 7, 123
BsuI GTATCC 1 cut(s) 220
BsuRI GGCC 2 cut(s) 355, 427
BtgI CCRYGG 1 cut(s) 356
BtsCI GGATG 3 cut(s) 55, 104, 443
BtsI GCAGTG 1 cut(s) 78
BtsIMutI CAGTG 1 cut(s) 78
CfoI GCGC 1 cut(s) 532
Cfr13I GGNCC 1 cut(s) 194
CseI GACGC 1 cut(s) 29
CviAII CATG 4 cut(s) 24, 322, 357, 414
CviJI RGCY 9 cut(s) 46, 53, 95, 116, 180, 355, 364, 427, 489
CviKI_1 RGCY 9 cut(s) 46, 53, 95, 116, 180, 355, 364, 427, 489
DdeI CTNAG 1 cut(s) 117
DpnI GATC 5 cut(s) 9, 77, 125, 535, 545
DpnII GATC 5 cut(s) 7, 75, 123, 533, 543
DrdI GACNNNNNNGTC 1 cut(s) 201
DseDI GACNNNNNNGTC 1 cut(s) 201
Eco130I CCWWGG 3 cut(s) 69, 302, 356
Eco47I GGWCC 1 cut(s) 194
EcoT14I CCWWGG 3 cut(s) 69, 302, 356
ErhI CCWWGG 3 cut(s) 69, 302, 356
FaeI CATG 4 cut(s) 27, 325, 360, 417
FatI CATG 4 cut(s) 23, 321, 356, 413
FbaI TGATCA 1 cut(s) 543
Fnu4HI GCNGC 1 cut(s) 487
FokI GGATG 3 cut(s) 42, 111, 450
Fsp4HI GCNGC 1 cut(s) 487
FspBI CTAG 1 cut(s) 365
GlaI GCGC 1 cut(s) 531
GluI GCNGC 1 cut(s) 487
GsuI CTGGAG 2 cut(s) 150, 526
HaeIII GGCC 2 cut(s) 355, 427
HapII CCGG 2 cut(s) 151, 539
HgaI GACGC 1 cut(s) 29
HhaI GCGC 1 cut(s) 532
Hin1II CATG 4 cut(s) 27, 325, 360, 417
Hin6I GCGC 1 cut(s) 530
HinP1I GCGC 1 cut(s) 530
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HinfI GANTC 2 cut(s) 40, 142
HpaI GTTAAC 1 cut(s) 408
HpaII CCGG 2 cut(s) 151, 539
HphI GGTGA 1 cut(s) 553
Hpy166II GTNNAC 1 cut(s) 408
Hpy188I TCNGA 4 cut(s) 7, 39, 120, 519
Hpy188III TCNNGA 2 cut(s) 129, 151
Hpy8I GTNNAC 1 cut(s) 408
Hpy99I CGWCG 1 cut(s) 497
HpyAV CCTTC 3 cut(s) 241, 341, 345
HpyCH4III ACNGT 1 cut(s) 262
HpyCH4V TGCA 1 cut(s) 83
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 80, 361, 370
HpyF3I CTNAG 1 cut(s) 117
Hsp92II CATG 4 cut(s) 27, 325, 360, 417
HspAI GCGC 1 cut(s) 530
Kpn2I TCCGGA 1 cut(s) 150
Ksp22I TGATCA 1 cut(s) 543
KspAI GTTAAC 1 cut(s) 408
Kzo9I GATC 5 cut(s) 7, 75, 123, 533, 543
LmnI GCTCC 2 cut(s) 283, 507
LpnPI CCDG 8 cut(s) 69, 81, 114, 164, 409, 416, 490, 552
Lsp1109I GCAGC 1 cut(s) 498
LweI GCATC 1 cut(s) 97
MaeI CTAG 1 cut(s) 365
MaeIII GTNAC 1 cut(s) 203
MalI GATC 5 cut(s) 9, 77, 125, 535, 545
MboI GATC 5 cut(s) 7, 75, 123, 533, 543
MboII GAAGA 2 cut(s) 133, 256
MflI RGATCY 2 cut(s) 7, 123
MluCI AATT 5 cut(s) 218, 253, 310, 445, 467
MluI ACGCGT 1 cut(s) 552
MmeI TCCRAC 1 cut(s) 261
MnlI CCTC 3 cut(s) 185, 471, 508
MroI TCCGGA 1 cut(s) 150
MseI TTAA 3 cut(s) 135, 407, 444
MspI CCGG 2 cut(s) 151, 539
MvnI CGCG 2 cut(s) 532, 554
MwoI GCNNNNNNNGC 4 cut(s) 20, 80, 361, 370
NcoI CCATGG 1 cut(s) 356
NdeII GATC 5 cut(s) 7, 75, 123, 533, 543
NlaIII CATG 4 cut(s) 27, 325, 360, 417
NmuCI GTSAC 1 cut(s) 203
PfeI GAWTC 2 cut(s) 40, 142
PkrI GCNGC 1 cut(s) 488
PspPI GGNCC 1 cut(s) 194
PstI CTGCAG 1 cut(s) 85
PsuI RGATCY 2 cut(s) 7, 123
SaqAI TTAA 3 cut(s) 135, 407, 444
SatI GCNGC 1 cut(s) 487
Sau3AI GATC 5 cut(s) 7, 75, 123, 533, 543
Sau96I GGNCC 1 cut(s) 194
SetI ASST 7 cut(s) 48, 55, 97, 118, 276, 366, 491
SfaNI GCATC 1 cut(s) 97
SfcI CTRYAG 2 cut(s) 81, 111
SinI GGWCC 1 cut(s) 194
Sse9I AATT 5 cut(s) 218, 253, 310, 445, 467
SsiI CCGC 1 cut(s) 411
SspMI CTAG 1 cut(s) 365
StyI CCWWGG 3 cut(s) 69, 302, 356
TaaI ACNGT 1 cut(s) 262
TasI AATT 5 cut(s) 218, 253, 310, 445, 467
TfiI GAWTC 2 cut(s) 40, 142
Tru1I TTAA 3 cut(s) 135, 407, 444
Tru9I TTAA 3 cut(s) 135, 407, 444
TscAI CASTG 1 cut(s) 85
TseFI GTSAC 1 cut(s) 203
TseI GCWGC 1 cut(s) 486
Tsp45I GTSAC 1 cut(s) 203
TspDTI ATGAA 1 cut(s) 310
TspGWI ACGGA 2 cut(s) 154, 461
TspRI CASTG 1 cut(s) 85
VpaK11BI GGWCC 1 cut(s) 194
XspI CTAG 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.