AT4G20040

Pectin lyase fold virulence factor (InterPro IPR011050), Parallel beta-helix repeat (InterPro IPR006626)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0016157
Physical Location & Seq
Reverse (-)
10847299 .. 10849115
1817 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G20040.1

Sequence Viewer

Length: 1452 bp
ATGAGTCTCTATAGCTTCATTAGCTTGTTGTTACTGTTTCTCATCACGATTTCAATGGAGTTTAAAGAGACATCATCCTTAAGAAGAGACATGACCAAGCTTTTAAAGCTCCAAGAGAAGATTCAAGAAAGACTCGCAGTCACTCCCACTCTCTCACCAGTGTCTTCTCCTTCTTCTCACTCTCCCAAAATGGTTGGGAAGGTGATATACCCGATTGGTTACGGAGCTGACCCTACAGGTGGACAAGACAGTAGTGACGCAATACTTGAAGCTTTAACTGATGCTTTTCAGTTACAAACTGGACTTGAGATGCTGCCACGTGTCGCTGATTTAGGTGGTCTAGTTATTGATCTCCAAGGTGGCAGTTACATGATCGGAAAACCTCTCAGGTTCCCTTCCTCCGGTGGCGGAAATCTTGTGGTGAAAGGTGGGACTTTCCGGGCATCAGAACTGTTTCCTGGTGATAGGCATTTAGTTGAGCTTGTAGCATCAAACGCTAAGAAACCAATGAAAATGTCGCCAGAGGAGTCTTTCTCCGATCAGAAAGACCAGAGCTCTGGGATCTTCTATGAGGATGTGACCTTCCAAGATGTTCTCTTCGACTCAAGATTCCGAGGAGGTGGTATTTTGGTGATTGACTCAGCTCGTATCCGTATAACCAATTGTTATTTCCTCCATTTCACAACGCAAGGGATTAAAGTCCAAGGAGGTCATGAGACATACATTTCAAACTCCTTCTTGGGGCAACATTCAACGGTAGGAGGAGACAGAGAAGAAAGAGGATTTACTGGGACAGGAATCGATATCTCGAGTAACGACAATGCCATTACGGATGTAGTGATTTTCTCGGCTGGTATCGGTATCTCTTTGAATGGTGGTGCAAATATGGTTACGGGTGTGCATTGCTACAATAAAGCTACATGGTTTGGTGGCATTGGCATTCTAGTGAAATCACATTTAACGAGGATAGATAATTGTTACCTTGATTACACAGGTATAGTCATAGAAGACCCTGTCCATGTTCATGTCACCAATGCTTTGTTCTTAGGAGATGCAAACATCGTCTTGAGATCGGTACACGGGAAGATTTCCGGTGTAAACATCGTCAACAACATGTTTAGCGGTACCGCCAAAAACAACTTCCCGATTGTCAAGCTAGAAGGAGAGTTTCATGATATTAATCAAGTTGTGATTGATCAAAACAATGCAGAAGGGATGATGCTGAAATCAACAACCGGAAAGGCCATGGTTTCCGCAAATGGAACACGATGGATCGCTGATTTTTCCCCTGTTTTGGTATTCCCTAACCGGATAAACCATTACCAGCATTCGTTTTTCGCTCAATCCGGACAGATCCCTGCAAATGCAGTGACCAATGTCTCCAACAATATGGTGGTTGTAGAAACCGATAGAGCTGTAACTGGAACCGTGTCAATAATTGCTTATCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

483

Amino Acids

52.35

Weight (kDa)

6.01

Isoelectric Point (pI)

35.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta_helix PF13229 193 - 326 8.1e-07 Right handed beta helix region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20040
fragaria_vesca FvH4_3g12830
malus_domestica MD05G1247600.v1.1
prunus_persica Prupe.4G116400_v2.0.a1
pyrus_communis pycom05g22370
rosa_chinensis RchiOBHm_Chr5g0020771
rosa_laevigata RLG00000032561
rosa_multiflora Rmu_sc0002527.1_g000005
rosa_roxburghii Rroxscaffold_1G00057530
rosa_rugosa Rorug05G0062800
rosa_samantha Rh5AG152700 Rh5BG152400 Rh5CG164000 Rh5DG151900
rosa_wichuraiana Rw5G013540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1124
AccB1I GGYRCC 1 cut(s) 1124
AccIII TCCGGA 1 cut(s) 1346
AciI CCGC 4 cut(s) 408, 1122, 1128, 1254
AclWI GGATC 3 cut(s) 569, 1280, 1348
AcvI CACGTG 1 cut(s) 320
AfaI GTAC 2 cut(s) 1077, 1126
AfiI CCNNNNNNNGG 4 cut(s) 239, 401, 741, 1294
AflII CTTAAG 1 cut(s) 79
AflIII ACRYGT 2 cut(s) 319, 1113
AgsI TTSAA 6 cut(s) 54, 125, 269, 729, 753, 871
AhdI GACNNNNNGTC 1 cut(s) 137
AjnI CCWGG 1 cut(s) 457
AjuI GAANNNNNNNTTGG 8 cut(s) 105, 137, 653, 685, 1025, 1057, 1124, 1156
Alw21I GWGCWC 1 cut(s) 557
Alw26I GTCTC 6 cut(s) 11, 62, 81, 710, 759, 1384
AlwI GGATC 3 cut(s) 569, 1280, 1348
Ama87I CYCGRG 1 cut(s) 808
Aor13HI TCCGGA 1 cut(s) 1346
AoxI GGCC 1 cut(s) 1242
ApeKI GCWGC 1 cut(s) 313
AseI ATTAAT 1 cut(s) 1179
Asp700I GAANNNNTTC 1 cut(s) 453
Asp718I GGTACC 1 cut(s) 1124
AsuC2I CCSGG 1 cut(s) 440
AsuHPI GGTGA 6 cut(s) 147, 214, 433, 473, 643, 1021
AvaI CYCGRG 1 cut(s) 808
BanI GGYRCC 1 cut(s) 1124
BanII GRGCYC 1 cut(s) 557
BarI GAAGNNNNNNTAC 2 cut(s) 191, 223
BbrPI CACGTG 1 cut(s) 320
BbsI GAAGAC 2 cut(s) 156, 1014
Bbv12I GWGCWC 1 cut(s) 557
BbvI GCAGC 1 cut(s) 300
BccI CCATC 1 cut(s) 1263
BciT130I CCWGG 1 cut(s) 459
BciVI GTATCC 1 cut(s) 659
BclI TGATCA 1 cut(s) 1195
BcnI CCSGG 1 cut(s) 440
BcoDI GTCTC 6 cut(s) 11, 62, 81, 710, 759, 1384
BfaI CTAG 3 cut(s) 341, 944, 1157
BfmI CTRYAG 2 cut(s) 10, 234
BfrI CTTAAG 1 cut(s) 79
BfuI GTATCC 1 cut(s) 659
BisI GCNGC 1 cut(s) 314
BlsI GCNGC 1 cut(s) 315
Bme1390I CCNGG 2 cut(s) 440, 459
BmeRI GACNNNNNGTC 1 cut(s) 137
BmeT110I CYCGRG 1 cut(s) 808
BmiI GGNNCC 3 cut(s) 392, 1126, 1426
BmrFI CCNGG 2 cut(s) 440, 459
BmrI ACTGGG 1 cut(s) 798
BmsI GCATC 6 cut(s) 271, 300, 452, 497, 1042, 1209
BmuI ACTGGG 1 cut(s) 798
BoxI GACNNNNGTC 1 cut(s) 1376
BpiI GAAGAC 2 cut(s) 156, 1014
BplI GAGNNNNNCTC 2 cut(s) 518, 550
BpuEI CTTGAG 3 cut(s) 326, 589, 1087
BpuMI CCSGG 1 cut(s) 440
Bsa29I ATCGAT 1 cut(s) 801
BsaAI YACGTR 1 cut(s) 320
BsaBI GATNNNNATC 1 cut(s) 1179
BsaJI CCNNGG 4 cut(s) 355, 613, 703, 1245
BsaWI WCCGGW 5 cut(s) 401, 1091, 1235, 1308, 1346
Bsc4I CCNNNNNNNGG 4 cut(s) 239, 401, 741, 1294
Bse1I ACTGG 4 cut(s) 158, 304, 793, 1426
Bse3DI GCAATG 1 cut(s) 901
Bse8I GATNNNNATC 1 cut(s) 1179
BseAI TCCGGA 1 cut(s) 1346
BseBI CCWGG 1 cut(s) 459
BseCI ATCGAT 1 cut(s) 801
BseDI CCNNGG 4 cut(s) 355, 613, 703, 1245
BseGI GGATG 4 cut(s) 74, 580, 838, 1221
BseJI GATNNNNATC 1 cut(s) 1179
BseLI CCNNNNNNNGG 4 cut(s) 239, 401, 741, 1294
BseMI GCAATG 1 cut(s) 901
BseMII CTCAG 2 cut(s) 400, 654
BseNI ACTGG 4 cut(s) 158, 304, 793, 1426
BseRI GAGGAG 3 cut(s) 539, 630, 777
BseXI GCAGC 1 cut(s) 300
BshFI GGCC 1 cut(s) 1244
BshNI GGYRCC 1 cut(s) 1124
BshVI ATCGAT 1 cut(s) 801
BsiHKAI GWGCWC 1 cut(s) 557
BsiHKCI CYCGRG 1 cut(s) 808
BsiSI CCGG 6 cut(s) 402, 439, 1092, 1236, 1309, 1347
BslFI GGGAC 2 cut(s) 445, 805
BslI CCNNNNNNNGG 4 cut(s) 239, 401, 741, 1294
BsmAI GTCTC 6 cut(s) 11, 62, 81, 710, 759, 1384
BsmFI GGGAC 2 cut(s) 445, 805
BsmI GAATGC 2 cut(s) 939, 1327
BsnI GGCC 1 cut(s) 1244
BsoBI CYCGRG 1 cut(s) 808
Bsp1286I GDGCHC 1 cut(s) 557
Bsp13I TCCGGA 1 cut(s) 1346
Bsp143I GATC 8 cut(s) 349, 372, 538, 561, 1070, 1195, 1272, 1353
Bsp19I CCATGG 1 cut(s) 1245
BspACI CCGC 4 cut(s) 408, 1122, 1128, 1254
BspANI GGCC 1 cut(s) 1244
BspCNI CTCAG 2 cut(s) 399, 653
BspDI ATCGAT 1 cut(s) 801
BspEI TCCGGA 1 cut(s) 1346
BspHI TCATGA 2 cut(s) 712, 1171
BspLI GGNNCC 3 cut(s) 392, 1126, 1426
BspPI GGATC 3 cut(s) 569, 1280, 1348
BspT107I GGYRCC 1 cut(s) 1124
BspTI CTTAAG 1 cut(s) 79
BsrDI GCAATG 1 cut(s) 901
BsrI ACTGG 4 cut(s) 158, 304, 793, 1426
BssECI CCNNGG 4 cut(s) 355, 613, 703, 1245
BssMI GATC 8 cut(s) 349, 372, 538, 561, 1070, 1195, 1272, 1353
BssT1I CCWWGG 3 cut(s) 355, 703, 1245
Bst2UI CCWGG 1 cut(s) 459
Bst4CI ACNGT 5 cut(s) 36, 251, 453, 757, 1429
Bst6I CTCTTC 2 cut(s) 79, 602
BstAFI CTTAAG 1 cut(s) 79
BstBAI YACGTR 1 cut(s) 320
BstDEI CTNAG 4 cut(s) 386, 498, 640, 1045
BstDSI CCRYGG 1 cut(s) 1245
BstF5I GGATG 4 cut(s) 74, 580, 838, 1221
BstKTI GATC 8 cut(s) 352, 375, 541, 564, 1073, 1198, 1275, 1356
BstMAI GTCTC 6 cut(s) 11, 62, 81, 710, 759, 1384
BstMBI GATC 8 cut(s) 349, 372, 538, 561, 1070, 1195, 1272, 1353
BstMWI GCNNNNNNNGC 3 cut(s) 21, 106, 494
BstNI CCWGG 1 cut(s) 459
BstNSI RCATGY 1 cut(s) 1117
BstPAI GACNNNNGTC 1 cut(s) 1376
BstSCI CCNGG 2 cut(s) 438, 457
BstSFI CTRYAG 2 cut(s) 10, 234
BstV1I GCAGC 1 cut(s) 300
BstV2I GAAGAC 2 cut(s) 156, 1014
BstX2I RGATCY 2 cut(s) 561, 1353
BstXI CCANNNNNNTGG 2 cut(s) 557, 1390
BstYI RGATCY 2 cut(s) 561, 1353
Bsu15I ATCGAT 1 cut(s) 801
BsuI GTATCC 1 cut(s) 659
BsuRI GGCC 1 cut(s) 1244
BsuTUI ATCGAT 1 cut(s) 801
BtgI CCRYGG 1 cut(s) 1245
BtsCI GGATG 4 cut(s) 74, 580, 838, 1221
BtsI GCAGTG 1 cut(s) 1374
BtsIMutI CAGTG 2 cut(s) 165, 1374
CciI TCATGA 2 cut(s) 712, 1171
ClaI ATCGAT 1 cut(s) 801
CseI GACGC 1 cut(s) 266
Csp6I GTAC 2 cut(s) 1076, 1125
CviAII CATG 9 cut(s) 91, 370, 713, 921, 1019, 1025, 1114, 1172, 1246
CviQI GTAC 2 cut(s) 1076, 1125
DdeI CTNAG 4 cut(s) 386, 498, 640, 1045
DpnI GATC 8 cut(s) 351, 374, 540, 563, 1072, 1197, 1274, 1355
DpnII GATC 8 cut(s) 349, 372, 538, 561, 1070, 1195, 1272, 1353
DraI TTTAAA 2 cut(s) 64, 105
DriI GACNNNNNGTC 1 cut(s) 137
Eam1104I CTCTTC 2 cut(s) 79, 602
Eam1105I GACNNNNNGTC 1 cut(s) 137
EarI CTCTTC 2 cut(s) 79, 602
EciI GGCGGA 1 cut(s) 423
Ecl136II GAGCTC 1 cut(s) 555
Eco130I CCWWGG 3 cut(s) 355, 703, 1245
Eco24I GRGCYC 1 cut(s) 557
Eco32I GATATC 1 cut(s) 805
Eco53kI GAGCTC 1 cut(s) 555
Eco72I CACGTG 1 cut(s) 320
Eco88I CYCGRG 1 cut(s) 808
EcoICRI GAGCTC 1 cut(s) 555
EcoRII CCWGG 1 cut(s) 457
EcoRV GATATC 1 cut(s) 805
EcoT14I CCWWGG 3 cut(s) 355, 703, 1245
EcoT38I GRGCYC 1 cut(s) 557
ErhI CCWWGG 3 cut(s) 355, 703, 1245
FaeI CATG 9 cut(s) 94, 373, 716, 924, 1022, 1028, 1117, 1175, 1249
FaqI GGGAC 2 cut(s) 445, 805
FatI CATG 9 cut(s) 90, 369, 712, 920, 1018, 1024, 1113, 1171, 1245
FbaI TGATCA 1 cut(s) 1195
Fnu4HI GCNGC 1 cut(s) 314
FokI GGATG 4 cut(s) 61, 587, 845, 1228
FriOI GRGCYC 1 cut(s) 557
Fsp4HI GCNGC 1 cut(s) 314
FspBI CTAG 3 cut(s) 341, 944, 1157
GluI GCNGC 1 cut(s) 314
HaeIII GGCC 1 cut(s) 1244
HapII CCGG 6 cut(s) 402, 439, 1092, 1236, 1309, 1347
HgaI GACGC 1 cut(s) 266
Hin1II CATG 9 cut(s) 94, 373, 716, 924, 1022, 1028, 1117, 1175, 1249
HincII GTYRAC 1 cut(s) 1108
HindII GTYRAC 1 cut(s) 1108
HindIII AAGCTT 2 cut(s) 98, 270
HinfI GANTC 8 cut(s) 4, 121, 132, 527, 602, 609, 638, 798
HpaII CCGG 6 cut(s) 402, 439, 1092, 1236, 1309, 1347
HphI GGTGA 6 cut(s) 147, 214, 433, 473, 643, 1021
Hpy166II GTNNAC 4 cut(s) 242, 1078, 1099, 1108
Hpy188I TCNGA 5 cut(s) 377, 448, 538, 543, 614
Hpy188III TCNNGA 9 cut(s) 46, 125, 606, 713, 808, 1066, 1144, 1172, 1347
Hpy8I GTNNAC 4 cut(s) 242, 1078, 1099, 1108
HpyAV CCTTC 7 cut(s) 180, 193, 405, 592, 745, 1154, 1205
HpyCH4III ACNGT 5 cut(s) 36, 251, 453, 757, 1429
HpyCH4IV ACGT 1 cut(s) 319
HpyCH4V TGCA 6 cut(s) 881, 901, 1055, 1208, 1361, 1367
HpyF10VI GCNNNNNNNGC 3 cut(s) 21, 106, 494
HpyF3I CTNAG 4 cut(s) 386, 498, 640, 1045
HpySE526I ACGT 1 cut(s) 319
Hsp92II CATG 9 cut(s) 94, 373, 716, 924, 1022, 1028, 1117, 1175, 1249
Kpn2I TCCGGA 1 cut(s) 1346
KpnI GGTACC 1 cut(s) 1128
Ksp22I TGATCA 1 cut(s) 1195
Kzo9I GATC 8 cut(s) 349, 372, 538, 561, 1070, 1195, 1272, 1353
LmnI GCTCC 2 cut(s) 114, 224
Lsp1109I GCAGC 1 cut(s) 300
LweI GCATC 6 cut(s) 271, 300, 452, 497, 1042, 1209
MaeI CTAG 3 cut(s) 341, 944, 1157
MaeII ACGT 1 cut(s) 319
MalI GATC 8 cut(s) 351, 374, 540, 563, 1072, 1197, 1274, 1355
MboI GATC 8 cut(s) 349, 372, 538, 561, 1070, 1195, 1272, 1353
MboII GAAGA 9 cut(s) 96, 130, 156, 165, 556, 589, 785, 1019, 1096
MfeI CAATTG 1 cut(s) 661
MflI RGATCY 2 cut(s) 561, 1353
MhlI GDGCHC 1 cut(s) 557
MluCI AATT 3 cut(s) 661, 973, 1437
MlyI GAGTC 5 cut(s) 13, 126, 536, 596, 632
MmeI TCCRAC 1 cut(s) 1407
MroI TCCGGA 1 cut(s) 1346
MroXI GAANNNNTTC 1 cut(s) 453
MseI TTAA 7 cut(s) 63, 80, 104, 275, 696, 959, 1179
MslI CAYNNNNRTG 2 cut(s) 944, 1023
MspCI CTTAAG 1 cut(s) 79
MspI CCGG 6 cut(s) 402, 439, 1092, 1236, 1309, 1347
MspR9I CCNGG 2 cut(s) 440, 459
MunI CAATTG 1 cut(s) 661
Mva1269I GAATGC 2 cut(s) 939, 1327
MvaI CCWGG 1 cut(s) 459
MwoI GCNNNNNNNGC 3 cut(s) 21, 106, 494
NciI CCSGG 1 cut(s) 440
NcoI CCATGG 1 cut(s) 1245
NdeII GATC 8 cut(s) 349, 372, 538, 561, 1070, 1195, 1272, 1353
NlaIII CATG 9 cut(s) 94, 373, 716, 924, 1022, 1028, 1117, 1175, 1249
NlaIV GGNNCC 3 cut(s) 392, 1126, 1426
NmeAIII GCCGAG 1 cut(s) 827
NmuCI GTSAC 5 cut(s) 139, 254, 577, 1027, 1369
NspI RCATGY 1 cut(s) 1117
PaeR7I CTCGAG 1 cut(s) 808
PagI TCATGA 2 cut(s) 712, 1171
PciI ACATGT 1 cut(s) 1113
PctI GAATGC 2 cut(s) 939, 1327
PdmI GAANNNNTTC 1 cut(s) 453
PfeI GAWTC 3 cut(s) 121, 609, 798
PflFI GACNNNGTC 1 cut(s) 1013
PkrI GCNGC 1 cut(s) 315
PleI GAGTC 5 cut(s) 12, 126, 535, 596, 632
PmaCI CACGTG 1 cut(s) 320
PmlI CACGTG 1 cut(s) 320
PpsI GAGTC 5 cut(s) 12, 126, 535, 596, 632
Ppu21I YACGTR 1 cut(s) 320
PscI ACATGT 1 cut(s) 1113
PshAI GACNNNNGTC 1 cut(s) 1376
PshBI ATTAAT 1 cut(s) 1179
Psp124BI GAGCTC 1 cut(s) 557
Psp6I CCWGG 1 cut(s) 457
PspCI CACGTG 1 cut(s) 320
PspGI CCWGG 1 cut(s) 457
PspN4I GGNNCC 3 cut(s) 392, 1126, 1426
PsuI RGATCY 2 cut(s) 561, 1353
PsyI GACNNNGTC 1 cut(s) 1013
RsaI GTAC 2 cut(s) 1077, 1126
RsaNI GTAC 2 cut(s) 1076, 1125
RseI CAYNNNNRTG 2 cut(s) 944, 1023
SacI GAGCTC 1 cut(s) 557
SaqAI TTAA 7 cut(s) 63, 80, 104, 275, 696, 959, 1179
SatI GCNGC 1 cut(s) 314
Sau3AI GATC 8 cut(s) 349, 372, 538, 561, 1070, 1195, 1272, 1353
SchI GAGTC 5 cut(s) 13, 126, 536, 596, 632
ScrFI CCNGG 2 cut(s) 440, 459
SduI GDGCHC 1 cut(s) 557
SfaNI GCATC 6 cut(s) 271, 300, 452, 497, 1042, 1209
SfcI CTRYAG 2 cut(s) 10, 234
Sfr274I CTCGAG 1 cut(s) 808
SlaI CTCGAG 1 cut(s) 808
SmiMI CAYNNNNRTG 2 cut(s) 944, 1023
SmlI CTYRAG 5 cut(s) 79, 305, 604, 808, 1066
SmoI CTYRAG 5 cut(s) 79, 305, 604, 808, 1066
Sse9I AATT 3 cut(s) 661, 973, 1437
SsiI CCGC 4 cut(s) 408, 1122, 1128, 1254
SspMI CTAG 3 cut(s) 341, 944, 1157
SstI GAGCTC 1 cut(s) 557
StyD4I CCNGG 2 cut(s) 438, 457
StyI CCWWGG 3 cut(s) 355, 703, 1245
TaaI ACNGT 5 cut(s) 36, 251, 453, 757, 1429
TaiI ACGT 1 cut(s) 322
TaqI TCGA 3 cut(s) 600, 801, 809
TasI AATT 3 cut(s) 661, 973, 1437
TfiI GAWTC 3 cut(s) 121, 609, 798
Tru1I TTAA 7 cut(s) 63, 80, 104, 275, 696, 959, 1179
Tru9I TTAA 7 cut(s) 63, 80, 104, 275, 696, 959, 1179
TscAI CASTG 2 cut(s) 165, 1374
TseFI GTSAC 5 cut(s) 139, 254, 577, 1027, 1369
TseI GCWGC 1 cut(s) 313
Tsp45I GTSAC 5 cut(s) 139, 254, 577, 1027, 1369
TspDTI ATGAA 4 cut(s) 7, 524, 1013, 1160
TspGWI ACGGA 3 cut(s) 237, 641, 845
TspRI CASTG 2 cut(s) 165, 1374
Tth111I GACNNNGTC 1 cut(s) 1013
Vha464I CTTAAG 1 cut(s) 79
VspI ATTAAT 1 cut(s) 1179
XceI RCATGY 1 cut(s) 1117
XcmI CCANNNNNNNNNTGG 1 cut(s) 1390
XhoI CTCGAG 1 cut(s) 808
XmnI GAANNNNTTC 1 cut(s) 453
XspI CTAG 3 cut(s) 341, 944, 1157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.