AT4G20170

cell wall biogenesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0011102
Physical Location & Seq
Forward (+)
10896579 .. 10899141
2563 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G20170.1

Sequence Viewer

Length: 1515 bp
ATGGCCATGGTCAAAGAGAAAGAACAAAACACTAAAGACAAAAAACTCCTCGTCGGCGTCATTTGGAACTTCTCCGCCGAGCTCAAGCTCACTTTCATGGCGTTACTTGTTCTCTGCACTTTAGCTACTCTCTTACCTTTCATACCTTCTTCATTCTCTCTCTCCACTTCCGATTTCCGCTTCTGCATCTCACGCTTCTCCTCCGCCGTCCCTCTCAACACCACCACCACCGTAGAAGAATCATCATCCTCACCGTCACCGGAGAAGAACCTAGATCGAGTTTTGGATAACGGAGTTATTAAACGGACGTTTACTGGCTACGGCTCAGCAGCTTATAACTTCGTCTCAATGAGTGCTTACAGAGGCGGCGTTAACTCATTCGCCGTTATCGGATTATCATCAAAACCATTACACGTGTACGGTCATCCTTCGTATAGATGCGAATGGGTCTCATTAGACCCGACTCAAGATCCGATTTCAACAACCGGGTTTAAAATCTTAACCGATTGGGGTTACGGACGGATCTACACAACAGTCGTCGTTAACTGTACTTTCTCATCAATCTCCGCCGTGAATCCACAAAACTCCGGTGGAACTCTCATCCTCCACGCCACCACCGGAGATCCAACTCTCAATCTCACCGATTCAATCTCAGTCCTAACCGAACCTCCCAAATCCGTCGATTTCGATCTCTATAACTCCACGAAGAAGACGAAGAAGTACGATTATCTCTATTGCGGATCGTCCTTATACGGTAACCTAAGTCCGCAACGAGTTAGAGAATGGATCGCTTACCACGTTAGATTCTTCGGTGAACGGTCACATTTCGTGCTACACGACGCCGGAGGGATTCATGAGGAAGTGTTCGAGGTTTTAAAGCCATGGATTGAGCTAGGGAGAGTGACGTTACATGATATTAGAGATCAAGAACGATTCGATGGATATTATCATAATCAGTTCATGATAGTGAATGATTGTTTGCATAGGTATAGATTCATGACGAAGTGGATGTTCTTCTTTGATGTTGATGAGTTTTTACATGTTCCAGTGAAAGAGACGATTTCGTCTGTGATGGAATCTTTGGAGGAATATTCTCAGTTTACTATTGAACAGATGCCTATGAGTAGTCGGATTTGTTATTCCGGTGATGGTCCGGCGAGAACTTACAGGAAATGGGGAATTGAGAAACTGGCATATAGAGACGTCAAGAAGGTTCCAAGACGGGATCGAAAATACGCTGTCCAGCCGGAGAATGTATTCGCGACAGGCGTACACATGTCTCAGAATCTACAAGGGAAAACATACCACAAGGCTGAAAGCAAAATCCGTTACTTCCACTACCATGGTTCGATCTCTCAGCGCCGCGAGCCTTGTCGTCAACTTTTTAACGATTCTCGAGTCGTGTTCGAGAACACTCCTTATGTGCTAGACACTACAATATGTGATGTTGGCCTTGCTGTGAGAACGTTCGAGTTGAGAACGATCGGTGATCGGCTGCTACGGACAAGACAATGA

Protein Analysis

504

Amino Acids

57.78

Weight (kDa)

8.66

Isoelectric Point (pI)

39.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_92 PF01697 242 - 458 2.8e-31 Glycosyltransferase family 92
Glyco_tranf_2_4 PF13704 258 - 357 2.8e-08 Glycosyl transferase family 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011102)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20170 AT5G44670
fragaria_vesca FvH4_3g13330 FvH4_3g13330
malus_domestica MD05G1242800.v1.1 MD10G1224800.v1.1
prunus_persica Prupe.4G120600_v2.0.a1
pyrus_communis pycom05g21940 pycom10g18850
rosa_chinensis RchiOBHm_Chr5g0021801
rosa_laevigata RLG00000032629
rosa_multiflora Rmu_co8317171.1_g000001 Rmu_ssc0000023.1_g000003
rosa_roxburghii Rroxscaffold_1G00056920
rosa_rugosa Rorug05G0069600
rosa_samantha Rh5AG158100 Rh5BG157700 Rh5CG171800 Rh5DG157900
rosa_wichuraiana Rw5G014080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 336
AasI GACNNNNNNGTC 1 cut(s) 1063
AatII GACGTC 1 cut(s) 1206
AccII CGCG 2 cut(s) 1262, 1365
AciI CCGC 8 cut(s) 75, 178, 204, 366, 567, 738, 767, 1363
AclI AACGTT 1 cut(s) 1466
AclWI GGATC 6 cut(s) 464, 530, 617, 748, 794, 1233
AcoI YGGCCR 1 cut(s) 3
AcvI CACGTG 1 cut(s) 415
AcyI GRCGYC 3 cut(s) 57, 840, 1203
AfaI GTAC 4 cut(s) 419, 550, 722, 1272
AflIII ACRYGT 4 cut(s) 412, 414, 1039, 1275
AgsI TTSAA 3 cut(s) 480, 648, 1109
AluBI AGCT 5 cut(s) 82, 88, 125, 332, 892
AluI AGCT 5 cut(s) 82, 88, 125, 332, 892
Alw21I GWGCWC 1 cut(s) 84
Alw26I GTCTC 5 cut(s) 349, 454, 1049, 1194, 1284
AlwI GGATC 6 cut(s) 464, 530, 617, 748, 794, 1233
Ama87I CYCGRG 1 cut(s) 1395
AoxI GGCC 2 cut(s) 3, 1450
ApeKI GCWGC 2 cut(s) 329, 1495
Asp700I GAANNNNTTC 1 cut(s) 1256
AspLEI GCGC 1 cut(s) 1362
AspS9I GGNCC 1 cut(s) 1151
AsuC2I CCSGG 1 cut(s) 487
AsuHPI GGTGA 6 cut(s) 243, 249, 631, 824, 1157, 1499
AvaI CYCGRG 1 cut(s) 1395
AvaII GGWCC 1 cut(s) 1151
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 84
BbrPI CACGTG 1 cut(s) 415
BbsI GAAGAC 1 cut(s) 716
Bbv12I GWGCWC 1 cut(s) 84
BbvI GCAGC 2 cut(s) 341, 1482
BccI CCATC 3 cut(s) 932, 1066, 1142
BceAI ACGGC 4 cut(s) 191, 337, 368, 554
BcnI CCSGG 1 cut(s) 487
BcoDI GTCTC 5 cut(s) 349, 454, 1049, 1194, 1284
BfaI CTAG 3 cut(s) 272, 893, 1427
BfoI RGCGCY 1 cut(s) 1363
BisI GCNGC 4 cut(s) 330, 367, 1363, 1496
BlpI GCTNAGC 1 cut(s) 325
BlsI GCNGC 4 cut(s) 331, 368, 1364, 1497
Bme1390I CCNGG 1 cut(s) 487
Bme18I GGWCC 1 cut(s) 1151
BmeT110I CYCGRG 1 cut(s) 1395
BmgT120I GGNCC 1 cut(s) 1151
BmiI GGNNCC 1 cut(s) 1215
BmrFI CCNGG 1 cut(s) 487
BmsI GCATC 3 cut(s) 195, 428, 1104
BpiI GAAGAC 1 cut(s) 716
Bpu1102I GCTNAGC 1 cut(s) 325
BpuEI CTTGAG 2 cut(s) 68, 450
BpuMI CCSGG 1 cut(s) 487
BsaAI YACGTR 1 cut(s) 415
BsaBI GATNNNNATC 2 cut(s) 397, 687
BsaHI GRCGYC 3 cut(s) 57, 840, 1203
BsaI GGTCTC 1 cut(s) 454
BsaJI CCNNGG 3 cut(s) 6, 881, 1342
BsaWI WCCGGW 4 cut(s) 259, 587, 617, 1142
BsaXI ACNNNNNCTCC 2 cut(s) 652, 682
Bse1I ACTGG 3 cut(s) 319, 1046, 1194
Bse8I GATNNNNATC 2 cut(s) 397, 687
BseDI CCNNGG 3 cut(s) 6, 881, 1342
BseGI GGATG 4 cut(s) 245, 424, 600, 1014
BseJI GATNNNNATC 2 cut(s) 397, 687
BseMII CTCAG 5 cut(s) 339, 666, 1109, 1295, 1370
BseNI ACTGG 3 cut(s) 319, 1046, 1194
BseRI GAGGAG 2 cut(s) 38, 190
BseXI GCAGC 2 cut(s) 341, 1482
BsgI GTGCAG 1 cut(s) 100
Bsh1236I CGCG 2 cut(s) 1262, 1365
Bsh1285I CGRYCG 1 cut(s) 1485
BshFI GGCC 2 cut(s) 5, 1452
BsiEI CGRYCG 1 cut(s) 1485
BsiHKAI GWGCWC 1 cut(s) 84
BsiHKCI CYCGRG 1 cut(s) 1395
BsiSI CCGG 8 cut(s) 260, 486, 588, 618, 843, 1143, 1154, 1247
BslFI GGGAC 1 cut(s) 194
BsmAI GTCTC 5 cut(s) 349, 454, 1049, 1194, 1284
BsmBI CGTCTC 3 cut(s) 349, 1049, 1194
BsmFI GGGAC 1 cut(s) 194
BsnI GGCC 2 cut(s) 5, 1452
Bso31I GGTCTC 1 cut(s) 454
BsoBI CYCGRG 1 cut(s) 1395
Bsp1286I GDGCHC 1 cut(s) 84
Bsp1720I GCTNAGC 1 cut(s) 325
Bsp19I CCATGG 3 cut(s) 6, 881, 1342
Bsp68I TCGCGA 1 cut(s) 1262
BspACI CCGC 8 cut(s) 75, 178, 204, 366, 567, 738, 767, 1363
BspANI GGCC 2 cut(s) 5, 1452
BspCNI CTCAG 5 cut(s) 338, 665, 1108, 1294, 1369
BspFNI CGCG 2 cut(s) 1262, 1365
BspHI TCATGA 3 cut(s) 853, 960, 996
BspLI GGNNCC 1 cut(s) 1215
BspPI GGATC 6 cut(s) 464, 530, 617, 748, 794, 1233
BspTNI GGTCTC 1 cut(s) 454
BsrI ACTGG 3 cut(s) 319, 1046, 1194
BssECI CCNNGG 3 cut(s) 6, 881, 1342
BssNI GRCGYC 3 cut(s) 57, 840, 1203
BssT1I CCWWGG 3 cut(s) 6, 881, 1342
Bst4CI ACNGT 7 cut(s) 232, 255, 422, 535, 548, 755, 819
BstACI GRCGYC 3 cut(s) 57, 840, 1203
BstBAI YACGTR 1 cut(s) 415
BstC8I GCNNGC 1 cut(s) 1367
BstDEI CTNAG 6 cut(s) 325, 652, 761, 1095, 1281, 1356
BstDSI CCRYGG 3 cut(s) 6, 881, 1342
BstEII GGTNACC 1 cut(s) 755
BstF5I GGATG 4 cut(s) 245, 424, 600, 1014
BstFNI CGCG 2 cut(s) 1262, 1365
BstH2I RGCGCY 1 cut(s) 1363
BstHHI GCGC 1 cut(s) 1362
BstMAI GTCTC 5 cut(s) 349, 454, 1049, 1194, 1284
BstMCI CGRYCG 1 cut(s) 1485
BstMWI GCNNNNNNNGC 2 cut(s) 192, 1366
BstNSI RCATGY 2 cut(s) 1043, 1279
BstPI GGTNACC 1 cut(s) 755
BstSCI CCNGG 1 cut(s) 485
BstUI CGCG 2 cut(s) 1262, 1365
BstV1I GCAGC 2 cut(s) 341, 1482
BstV2I GAAGAC 1 cut(s) 716
BstX2I RGATCY 3 cut(s) 469, 522, 622
BstXI CCANNNNNNTGG 1 cut(s) 1343
BstYI RGATCY 3 cut(s) 469, 522, 622
BsuRI GGCC 2 cut(s) 5, 1452
BtgI CCRYGG 3 cut(s) 6, 881, 1342
BtsCI GGATG 4 cut(s) 245, 424, 600, 1014
BtsIMutI CAGTG 1 cut(s) 1053
BtuMI TCGCGA 1 cut(s) 1262
Cac8I GCNNGC 1 cut(s) 1367
CciI TCATGA 3 cut(s) 853, 960, 996
CfoI GCGC 1 cut(s) 1362
Cfr13I GGNCC 1 cut(s) 1151
CseI GACGC 2 cut(s) 46, 848
Csp6I GTAC 4 cut(s) 418, 549, 721, 1271
CviQI GTAC 4 cut(s) 418, 549, 721, 1271
DdeI CTNAG 6 cut(s) 325, 652, 761, 1095, 1281, 1356
DraI TTTAAA 2 cut(s) 493, 876
DrdI GACNNNNNNGTC 1 cut(s) 1063
DseDI GACNNNNNNGTC 1 cut(s) 1063
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 3 cut(s) 64, 193, 556
Ecl136II GAGCTC 1 cut(s) 82
Eco130I CCWWGG 3 cut(s) 6, 881, 1342
Eco24I GRGCYC 1 cut(s) 84
Eco31I GGTCTC 1 cut(s) 454
Eco47I GGWCC 1 cut(s) 1151
Eco53kI GAGCTC 1 cut(s) 82
Eco72I CACGTG 1 cut(s) 415
Eco88I CYCGRG 1 cut(s) 1395
Eco91I GGTNACC 1 cut(s) 755
EcoICRI GAGCTC 1 cut(s) 82
EcoO65I GGTNACC 1 cut(s) 755
EcoT14I CCWWGG 3 cut(s) 6, 881, 1342
EcoT38I GRGCYC 1 cut(s) 84
ErhI CCWWGG 3 cut(s) 6, 881, 1342
Esp3I CGTCTC 3 cut(s) 349, 1049, 1194
FaqI GGGAC 1 cut(s) 194
Fnu4HI GCNGC 4 cut(s) 330, 367, 1363, 1496
FokI GGATG 4 cut(s) 232, 411, 587, 1021
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 4 cut(s) 330, 367, 1363, 1496
FspBI CTAG 3 cut(s) 272, 893, 1427
GlaI GCGC 1 cut(s) 1361
GluI GCNGC 4 cut(s) 330, 367, 1363, 1496
HaeII RGCGCY 1 cut(s) 1363
HaeIII GGCC 2 cut(s) 5, 1452
HapII CCGG 8 cut(s) 260, 486, 588, 618, 843, 1143, 1154, 1247
HgaI GACGC 2 cut(s) 46, 848
HhaI GCGC 1 cut(s) 1362
Hin1I GRCGYC 3 cut(s) 57, 840, 1203
Hin6I GCGC 1 cut(s) 1360
HinP1I GCGC 1 cut(s) 1360
HincII GTYRAC 3 cut(s) 373, 544, 1379
HindII GTYRAC 3 cut(s) 373, 544, 1379
HpaI GTTAAC 2 cut(s) 373, 544
HpaII CCGG 8 cut(s) 260, 486, 588, 618, 843, 1143, 1154, 1247
HphI GGTGA 6 cut(s) 243, 249, 631, 824, 1157, 1499
Hpy166II GTNNAC 8 cut(s) 312, 373, 418, 544, 815, 1101, 1273, 1379
Hpy188I TCNGA 5 cut(s) 172, 392, 474, 1131, 1284
Hpy188III TCNNGA 9 cut(s) 467, 854, 926, 961, 997, 1207, 1261, 1395, 1408
Hpy8I GTNNAC 8 cut(s) 312, 373, 418, 544, 815, 1101, 1273, 1379
Hpy99I CGWCG 4 cut(s) 56, 542, 683, 842
HpyAV CCTTC 3 cut(s) 156, 438, 1204
HpyCH4III ACNGT 7 cut(s) 232, 255, 422, 535, 548, 755, 819
HpyCH4IV ACGT 6 cut(s) 308, 414, 798, 905, 1203, 1466
HpyCH4V TGCA 3 cut(s) 117, 186, 982
HpyF10VI GCNNNNNNNGC 2 cut(s) 192, 1366
HpyF3I CTNAG 6 cut(s) 325, 652, 761, 1095, 1281, 1356
HpySE526I ACGT 6 cut(s) 308, 414, 798, 905, 1203, 1466
Hsp92I GRCGYC 3 cut(s) 57, 840, 1203
HspAI GCGC 1 cut(s) 1360
KspAI GTTAAC 2 cut(s) 373, 544
Lsp1109I GCAGC 2 cut(s) 341, 1482
LweI GCATC 3 cut(s) 195, 428, 1104
MaeI CTAG 3 cut(s) 272, 893, 1427
MaeII ACGT 6 cut(s) 308, 414, 798, 905, 1203, 1466
MaeIII GTNAC 8 cut(s) 102, 255, 512, 755, 819, 901, 906, 1328
MboII GAAGA 8 cut(s) 141, 248, 277, 718, 721, 727, 799, 1006
MflI RGATCY 3 cut(s) 469, 522, 622
MhlI GDGCHC 1 cut(s) 84
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 1179
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 457, 1407
MmeI TCCRAC 2 cut(s) 650, 1109
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 1256
MscI TGGCCA 1 cut(s) 5
MseI TTAA 7 cut(s) 300, 372, 492, 500, 543, 875, 1386
MslI CAYNNNNRTG 3 cut(s) 95, 965, 1341
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 8 cut(s) 260, 486, 588, 618, 843, 1143, 1154, 1247
MspR9I CCNGG 1 cut(s) 487
MvnI CGCG 2 cut(s) 1262, 1365
MwoI GCNNNNNNNGC 2 cut(s) 192, 1366
NciI CCSGG 1 cut(s) 487
NcoI CCATGG 3 cut(s) 6, 881, 1342
NlaIV GGNNCC 1 cut(s) 1215
NmeAIII GCCGAG 1 cut(s) 103
NmuCI GTSAC 3 cut(s) 255, 819, 901
NruI TCGCGA 1 cut(s) 1262
NspI RCATGY 2 cut(s) 1043, 1279
PaeR7I CTCGAG 1 cut(s) 1395
PagI TCATGA 3 cut(s) 853, 960, 996
PciI ACATGT 2 cut(s) 1039, 1275
PcsI WCGNNNNNNNCGW 4 cut(s) 710, 795, 834, 1266
PdmI GAANNNNTTC 1 cut(s) 1256
PkrI GCNGC 4 cut(s) 331, 368, 1364, 1497
Ple19I CGATCG 1 cut(s) 1485
PleI GAGTC 2 cut(s) 457, 1406
PmaCI CACGTG 1 cut(s) 415
PmlI CACGTG 1 cut(s) 415
PpsI GAGTC 2 cut(s) 457, 1406
Ppu21I YACGTR 1 cut(s) 415
PscI ACATGT 2 cut(s) 1039, 1275
PsiI TTATAA 1 cut(s) 336
Psp124BI GAGCTC 1 cut(s) 84
Psp1406I AACGTT 1 cut(s) 1466
PspCI CACGTG 1 cut(s) 415
PspEI GGTNACC 1 cut(s) 755
PspN4I GGNNCC 1 cut(s) 1215
PspPI GGNCC 1 cut(s) 1151
PsuI RGATCY 3 cut(s) 469, 522, 622
PvuI CGATCG 1 cut(s) 1485
RruI TCGCGA 1 cut(s) 1262
RsaI GTAC 4 cut(s) 419, 550, 722, 1272
RsaNI GTAC 4 cut(s) 418, 549, 721, 1271
RseI CAYNNNNRTG 3 cut(s) 95, 965, 1341
SacI GAGCTC 1 cut(s) 84
SaqAI TTAA 7 cut(s) 300, 372, 492, 500, 543, 875, 1386
SatI GCNGC 4 cut(s) 330, 367, 1363, 1496
Sau96I GGNCC 1 cut(s) 1151
SchI GAGTC 2 cut(s) 457, 1407
ScrFI CCNGG 1 cut(s) 487
SduI GDGCHC 1 cut(s) 84
SfaNI GCATC 3 cut(s) 195, 428, 1104
Sfr274I CTCGAG 1 cut(s) 1395
SinI GGWCC 1 cut(s) 1151
SlaI CTCGAG 1 cut(s) 1395
SmiMI CAYNNNNRTG 3 cut(s) 95, 965, 1341
SmlI CTYRAG 3 cut(s) 83, 465, 1395
SmoI CTYRAG 3 cut(s) 83, 465, 1395
Sse9I AATT 1 cut(s) 1179
SsiI CCGC 8 cut(s) 75, 178, 204, 366, 567, 738, 767, 1363
SspI AATATT 1 cut(s) 1091
SspMI CTAG 3 cut(s) 272, 893, 1427
SstI GAGCTC 1 cut(s) 84
StyD4I CCNGG 1 cut(s) 485
StyI CCWWGG 3 cut(s) 6, 881, 1342
TaaI ACNGT 7 cut(s) 232, 255, 422, 535, 548, 755, 819
TaiI ACGT 6 cut(s) 311, 417, 801, 908, 1206, 1469
TasI AATT 1 cut(s) 1179
TatI WGTACW 1 cut(s) 548
TauI GCSGC 2 cut(s) 369, 1365
Tru1I TTAA 7 cut(s) 300, 372, 492, 500, 543, 875, 1386
Tru9I TTAA 7 cut(s) 300, 372, 492, 500, 543, 875, 1386
TscAI CASTG 1 cut(s) 1053
TseFI GTSAC 3 cut(s) 255, 819, 901
TseI GCWGC 2 cut(s) 329, 1495
Tsp45I GTSAC 3 cut(s) 255, 819, 901
TspDTI ATGAA 6 cut(s) 85, 130, 141, 842, 949, 985
TspGWI ACGGA 6 cut(s) 306, 319, 531, 535, 667, 1316
TspRI CASTG 1 cut(s) 1053
VpaK11BI GGWCC 1 cut(s) 1151
XceI RCATGY 2 cut(s) 1043, 1279
XhoI CTCGAG 1 cut(s) 1395
XmnI GAANNNNTTC 1 cut(s) 1256
XspI CTAG 3 cut(s) 272, 893, 1427
ZraI GACGTC 1 cut(s) 1204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.