AT4G30680

eukaryotic translation initiation factor isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0020266
Physical Location & Seq
Reverse (-)
14958687 .. 14960321
1635 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G30680.1

Sequence Viewer

Length: 792 bp
ATGCAGCAAGGTGACTCAATGCTGAGCTTGAGGCCTGGAGGTGGTCGTGGAGGAAGCAGACGCTTTGCCCCACGCTTTACCTTATCTTCTTCCTCTGATCTTACCAATGGCGGAGACGCTCCTTCTTTTGCCGTTAAGGGCAGTGGAGGCCTTCTGAACGATAGACCAAGTGCCCTTGTGCAAGGTAATGGTTCCCAACAGCCTAAACCTGTTCCTTCCCCGACCAGACAAACTGTGGAGAAGCCAAAACCACAACCACAGCCACAGGAGGTGGCTCCTCCTACGACTACTAGCTTGAACACAGTAGAGCTCAGTAGAAAGACCAACTCGCTTTTGGAGGAATATTTCAATGTCCGTCTATTGGATGAGGCATTACAATGCATAGAAGAACTGAAAACCCCGTCTTACCATCCTGAGCTTGTCAAGGAAGCTATCTCCCTCGGCTTGGAGAAGAACCCTCCTTGTGTTGAACCAGTGGCGAAGCTCTTGGAACATCTGGTCTCTAAGAATGTTCTAACTCCTAAAGACTTAAGGAATGGTTGTCTGCTCTATGGGTCTATGCTCGATGACATTGGGATCGATCTGCCAAAAGCGCCAAACAACTTTGGGGAGATCCTTGGGAGTTTGGTTATGGCCAAAGCATCGGATTCTGAGTTGGTGAAAGAGATTCTAATGAAAATGGGAGACGAATGGTTCAAGAAAGCAGTCTTGGAAGCTGTGATGAGGAGTGTTAGTGAGTCTCTACTCACTACGGAAGCAGTAGAGGTTGAGGCTTGCCGTGGTTTGGTGTAG

Protein Analysis

263

Amino Acids

28.39

Weight (kDa)

5.44

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MA3 PF02847 105 - 213 8.6e-29 MA3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020266)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30680
pyrus_communis pycom15g13490
rosa_chinensis RchiOBHm_Chr5g0028791
rosa_samantha Rh1BG001500 Rh1CG009100 Rh2DG419500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 111
AclWI GGATC 2 cut(s) 584, 607
AcoI YGGCCR 1 cut(s) 633
AfiI CCNNNNNNNGG 4 cut(s) 41, 361, 445, 784
AflII CTTAAG 1 cut(s) 529
AgsI TTSAA 4 cut(s) 298, 349, 470, 697
AjnI CCWGG 1 cut(s) 34
AjuI GAANNNNNNNTTGG 2 cut(s) 692, 724
AluBI AGCT 7 cut(s) 27, 294, 310, 418, 431, 484, 716
AluI AGCT 7 cut(s) 27, 294, 310, 418, 431, 484, 716
Alw21I GWGCWC 1 cut(s) 312
Alw26I GTCTC 4 cut(s) 108, 505, 678, 744
AlwI GGATC 2 cut(s) 584, 607
AoxI GGCC 3 cut(s) 32, 148, 633
ApeKI GCWGC 1 cut(s) 4
AspLEI GCGC 1 cut(s) 595
AsuHPI GGTGA 2 cut(s) 23, 670
BaeGI GKGCMC 1 cut(s) 175
BalI TGGCCA 1 cut(s) 635
BanII GRGCYC 1 cut(s) 312
Bbv12I GWGCWC 1 cut(s) 312
BbvI GCAGC 1 cut(s) 16
BccI CCATC 1 cut(s) 417
BceAI ACGGC 2 cut(s) 116, 762
BciT130I CCWGG 1 cut(s) 36
BcoDI GTCTC 4 cut(s) 108, 505, 678, 744
BfaI CTAG 1 cut(s) 291
BfoI RGCGCY 1 cut(s) 596
BfrI CTTAAG 1 cut(s) 529
BisI GCNGC 1 cut(s) 5
BlpI GCTNAGC 1 cut(s) 23
BlsI GCNGC 1 cut(s) 6
Bme1390I CCNGG 1 cut(s) 36
BmiI GGNNCC 2 cut(s) 193, 276
BmrFI CCNGG 1 cut(s) 36
BmsI GCATC 1 cut(s) 650
BpmI CTGGAG 1 cut(s) 57
Bpu10I CCTNAGC 1 cut(s) 414
Bpu1102I GCTNAGC 1 cut(s) 23
BpuEI CTTGAG 1 cut(s) 49
Bsa29I ATCGAT 1 cut(s) 579
BsaI GGTCTC 1 cut(s) 505
BsaJI CCNNGG 3 cut(s) 439, 616, 778
BsaXI ACNNNNNCTCC 2 cut(s) 718, 748
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 361, 445, 784
Bse1I ACTGG 1 cut(s) 473
BseBI CCWGG 1 cut(s) 36
BseCI ATCGAT 1 cut(s) 579
BseDI CCNNGG 3 cut(s) 439, 616, 778
BseGI GGATG 2 cut(s) 370, 409
BseLI CCNNNNNNNGG 4 cut(s) 41, 361, 445, 784
BseMII CTCAG 4 cut(s) 14, 325, 405, 642
BseNI ACTGG 1 cut(s) 473
BseRI GAGGAG 2 cut(s) 267, 739
BseSI GKGCMC 1 cut(s) 175
BseXI GCAGC 1 cut(s) 16
BshFI GGCC 3 cut(s) 34, 150, 635
BshVI ATCGAT 1 cut(s) 579
BsiHKAI GWGCWC 1 cut(s) 312
BslI CCNNNNNNNGG 4 cut(s) 41, 361, 445, 784
BsmAI GTCTC 4 cut(s) 108, 505, 678, 744
BsmBI CGTCTC 2 cut(s) 108, 678
BsnI GGCC 3 cut(s) 34, 150, 635
Bso31I GGTCTC 1 cut(s) 505
Bsp1286I GDGCHC 2 cut(s) 175, 312
Bsp143I GATC 4 cut(s) 97, 576, 580, 612
Bsp1720I GCTNAGC 1 cut(s) 23
BspACI CCGC 1 cut(s) 111
BspANI GGCC 3 cut(s) 34, 150, 635
BspCNI CTCAG 4 cut(s) 15, 324, 406, 643
BspDI ATCGAT 1 cut(s) 579
BspLI GGNNCC 2 cut(s) 193, 276
BspPI GGATC 2 cut(s) 584, 607
BspTI CTTAAG 1 cut(s) 529
BspTNI GGTCTC 1 cut(s) 505
BsrI ACTGG 1 cut(s) 473
BssECI CCNNGG 3 cut(s) 439, 616, 778
BssMI GATC 4 cut(s) 97, 576, 580, 612
BssT1I CCWWGG 1 cut(s) 616
Bst2UI CCWGG 1 cut(s) 36
Bst4CI ACNGT 2 cut(s) 235, 304
BstAFI CTTAAG 1 cut(s) 529
BstC8I GCNNGC 1 cut(s) 775
BstDEI CTNAG 5 cut(s) 23, 311, 414, 504, 651
BstDSI CCRYGG 1 cut(s) 778
BstF5I GGATG 2 cut(s) 370, 409
BstH2I RGCGCY 1 cut(s) 596
BstHHI GCGC 1 cut(s) 595
BstKTI GATC 4 cut(s) 100, 579, 583, 615
BstMAI GTCTC 4 cut(s) 108, 505, 678, 744
BstMBI GATC 4 cut(s) 97, 576, 580, 612
BstMWI GCNNNNNNNGC 2 cut(s) 147, 592
BstNI CCWGG 1 cut(s) 36
BstSCI CCNGG 1 cut(s) 34
BstSLI GKGCMC 1 cut(s) 175
BstV1I GCAGC 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 612
BstYI RGATCY 1 cut(s) 612
Bsu15I ATCGAT 1 cut(s) 579
BsuRI GGCC 3 cut(s) 34, 150, 635
BsuTUI ATCGAT 1 cut(s) 579
BtgI CCRYGG 1 cut(s) 778
BtsCI GGATG 2 cut(s) 370, 409
BtsI GCAGTG 1 cut(s) 148
BtsIMutI CAGTG 2 cut(s) 148, 480
Cac8I GCNNGC 1 cut(s) 775
CfoI GCGC 1 cut(s) 595
ClaI ATCGAT 1 cut(s) 579
CseI GACGC 2 cut(s) 69, 125
DdeI CTNAG 5 cut(s) 23, 311, 414, 504, 651
DpnI GATC 4 cut(s) 99, 578, 582, 614
DpnII GATC 4 cut(s) 97, 576, 580, 612
EaeI YGGCCR 1 cut(s) 633
EciI GGCGGA 1 cut(s) 126
Ecl136II GAGCTC 1 cut(s) 310
Eco130I CCWWGG 1 cut(s) 616
Eco147I AGGCCT 2 cut(s) 34, 150
Eco24I GRGCYC 1 cut(s) 312
Eco31I GGTCTC 1 cut(s) 505
Eco53kI GAGCTC 1 cut(s) 310
EcoICRI GAGCTC 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 34
EcoT14I CCWWGG 1 cut(s) 616
EcoT22I ATGCAT 1 cut(s) 383
EcoT38I GRGCYC 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 616
Esp3I CGTCTC 2 cut(s) 108, 678
FaiI YATR 4 cut(s) 383, 552, 560, 632
Fnu4HI GCNGC 1 cut(s) 5
FokI GGATG 2 cut(s) 377, 396
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 1 cut(s) 5
FspBI CTAG 1 cut(s) 291
GlaI GCGC 1 cut(s) 594
GluI GCNGC 1 cut(s) 5
GsuI CTGGAG 1 cut(s) 57
HaeII RGCGCY 1 cut(s) 596
HaeIII GGCC 3 cut(s) 34, 150, 635
HgaI GACGC 2 cut(s) 69, 125
HhaI GCGC 1 cut(s) 595
Hin6I GCGC 1 cut(s) 593
HinP1I GCGC 1 cut(s) 593
HinfI GANTC 4 cut(s) 14, 647, 667, 737
HphI GGTGA 2 cut(s) 23, 670
Hpy188I TCNGA 4 cut(s) 97, 156, 646, 652
Hpy188III TCNNGA 2 cut(s) 413, 697
HpyAV CCTTC 3 cut(s) 132, 161, 225
HpyCH4III ACNGT 2 cut(s) 235, 304
HpyCH4V TGCA 3 cut(s) 4, 181, 381
HpyF10VI GCNNNNNNNGC 2 cut(s) 147, 592
HpyF3I CTNAG 5 cut(s) 23, 311, 414, 504, 651
HspAI GCGC 1 cut(s) 593
Kzo9I GATC 4 cut(s) 97, 576, 580, 612
LmnI GCTCC 2 cut(s) 124, 280
LpnPI CCDG 8 cut(s) 21, 48, 222, 238, 251, 426, 482, 486
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 1 cut(s) 650
MaeI CTAG 1 cut(s) 291
MaeIII GTNAC 1 cut(s) 11
MalI GATC 4 cut(s) 99, 578, 582, 614
MboI GATC 4 cut(s) 97, 576, 580, 612
MboII GAAGA 4 cut(s) 78, 81, 398, 463
MflI RGATCY 1 cut(s) 612
MhlI GDGCHC 2 cut(s) 175, 312
MlsI TGGCCA 1 cut(s) 635
MluNI TGGCCA 1 cut(s) 635
MlyI GAGTC 2 cut(s) 8, 746
Mox20I TGGCCA 1 cut(s) 635
Mph1103I ATGCAT 1 cut(s) 383
MscI TGGCCA 1 cut(s) 635
MseI TTAA 2 cut(s) 135, 530
MslI CAYNNNNRTG 1 cut(s) 376
Msp20I TGGCCA 1 cut(s) 635
MspCI CTTAAG 1 cut(s) 529
MspR9I CCNGG 1 cut(s) 36
MvaI CCWGG 1 cut(s) 36
MwoI GCNNNNNNNGC 2 cut(s) 147, 592
NdeII GATC 4 cut(s) 97, 576, 580, 612
NlaIV GGNNCC 2 cut(s) 193, 276
NmeAIII GCCGAG 1 cut(s) 420
NmuCI GTSAC 1 cut(s) 11
NsiI ATGCAT 1 cut(s) 383
PceI AGGCCT 2 cut(s) 34, 150
PfeI GAWTC 2 cut(s) 647, 667
PkrI GCNGC 1 cut(s) 6
PleI GAGTC 2 cut(s) 8, 745
PpsI GAGTC 2 cut(s) 8, 745
Psp124BI GAGCTC 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 34
PspGI CCWGG 1 cut(s) 34
PspN4I GGNNCC 2 cut(s) 193, 276
PsuI RGATCY 1 cut(s) 612
RseI CAYNNNNRTG 1 cut(s) 376
SacI GAGCTC 1 cut(s) 312
SaqAI TTAA 2 cut(s) 135, 530
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 4 cut(s) 97, 576, 580, 612
SchI GAGTC 2 cut(s) 8, 746
ScrFI CCNGG 1 cut(s) 36
SduI GDGCHC 2 cut(s) 175, 312
SfaNI GCATC 1 cut(s) 650
SmiMI CAYNNNNRTG 1 cut(s) 376
SmlI CTYRAG 2 cut(s) 28, 529
SmoI CTYRAG 2 cut(s) 28, 529
SseBI AGGCCT 2 cut(s) 34, 150
SsiI CCGC 1 cut(s) 111
SspI AATATT 1 cut(s) 344
SspMI CTAG 1 cut(s) 291
SstI GAGCTC 1 cut(s) 312
StuI AGGCCT 2 cut(s) 34, 150
StyD4I CCNGG 1 cut(s) 34
StyI CCWWGG 1 cut(s) 616
TaaI ACNGT 2 cut(s) 235, 304
TaqI TCGA 2 cut(s) 564, 579
TfiI GAWTC 2 cut(s) 647, 667
Tru1I TTAA 2 cut(s) 135, 530
Tru9I TTAA 2 cut(s) 135, 530
TscAI CASTG 2 cut(s) 148, 480
TseFI GTSAC 1 cut(s) 11
TseI GCWGC 1 cut(s) 4
Tsp45I GTSAC 1 cut(s) 11
TspDTI ATGAA 1 cut(s) 689
TspGWI ACGGA 2 cut(s) 344, 767
TspRI CASTG 2 cut(s) 148, 480
Vha464I CTTAAG 1 cut(s) 529
XcmI CCANNNNNNNNNTGG 2 cut(s) 232, 331
XspI CTAG 1 cut(s) 291
Zsp2I ATGCAT 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.