Rh2DG419500

eukaryotic translation initiation factor isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0020266
Physical Location & Seq
Reverse (-)
60824032 .. 60824478
447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG419500.1

Sequence Viewer

Length: 447 bp
ATGTTAGATGAAGCATTACTGTGTGTGGAAGAGCTAAGATCTCCAGCTTACCACGAGATTGTCAAAGAAGCTATCTCCCTTGCGCTGGAGAAAAGTACATCCTGTGTTGAACCACTTTTTAAGCTTCTCAAGCATTTGTTCTCTAAGAAGGTCATTACAGCGAGTGACATAAGGAGTGGATGTTTGCTCTATGCGTCTTTGCTGAGTGATATTGGCATTGATTATCCTAAAGCACCGAGTTATTTTGGTGAAGTTATGGGAAAATTAATTTTAGTTGGAGCTTTGGATTTTAAGGTGGTGGAAGACGTTTTGAAGGAGGTGGATGACAACTACTATCAGAAAGCCATATTCAATGCAGGACTGCAGGTTATTAGTTCTAGCCCTTCCGGGCAAGCTGTGCTAGATTCACAAGCATCTGATATTGAGGCCTGCCGAAGTCTATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05416 map03013 map05416
Pfam Domains
Protein Families
MA3 MIF4G

Protein Analysis

148

Amino Acids

16.21

Weight (kDa)

4.78

Isoelectric Point (pI)

47.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MA3 PF02847 2 - 96 1.8e-19 MA3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020266)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30680
pyrus_communis pycom15g13490
rosa_chinensis RchiOBHm_Chr5g0028791
rosa_samantha Rh1BG001500 Rh1CG009100 Rh2DG419500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 355
AfaI GTAC 1 cut(s) 97
AfiI CCNNNNNNNGG 1 cut(s) 85
AgsI TTSAA 3 cut(s) 110, 313, 352
AluBI AGCT 6 cut(s) 34, 47, 71, 124, 281, 395
AluI AGCT 6 cut(s) 34, 47, 71, 124, 281, 395
AoxI GGCC 1 cut(s) 426
AseI ATTAAT 1 cut(s) 266
AspLEI GCGC 1 cut(s) 85
AsuC2I CCSGG 1 cut(s) 388
AsuHPI GGTGA 1 cut(s) 260
BauI CACGAG 1 cut(s) 53
BbsI GAAGAC 1 cut(s) 309
BcnI CCSGG 1 cut(s) 388
BfaI CTAG 3 cut(s) 378, 401, 445
BfmI CTRYAG 1 cut(s) 362
BfuAI ACCTGC 1 cut(s) 355
BglII AGATCT 1 cut(s) 38
Bme1390I CCNGG 1 cut(s) 388
BmrFI CCNGG 1 cut(s) 388
BmsI GCATC 1 cut(s) 422
BpiI GAAGAC 1 cut(s) 309
BpmI CTGGAG 2 cut(s) 27, 107
BpuEI CTTGAG 1 cut(s) 113
BpuMI CCSGG 1 cut(s) 388
BsaXI ACNNNNNCTCC 2 cut(s) 166, 196
Bsc4I CCNNNNNNNGG 1 cut(s) 85
BseGI GGATG 3 cut(s) 98, 185, 328
BseLI CCNNNNNNNGG 1 cut(s) 85
BseMII CTCAG 1 cut(s) 194
BshFI GGCC 1 cut(s) 428
BsiSI CCGG 1 cut(s) 387
BslI CCNNNNNNNGG 1 cut(s) 85
BsnI GGCC 1 cut(s) 428
Bsp143I GATC 1 cut(s) 38
BspANI GGCC 1 cut(s) 428
BspCNI CTCAG 1 cut(s) 195
BspMAI CTGCAG 1 cut(s) 366
BspMI ACCTGC 1 cut(s) 355
BspQI GCTCTTC 1 cut(s) 24
BssMI GATC 1 cut(s) 38
BssSI CACGAG 1 cut(s) 53
Bst2BI CACGAG 1 cut(s) 53
Bst4CI ACNGT 1 cut(s) 21
Bst6I CTCTTC 1 cut(s) 24
BstAPI GCANNNNNTGC 1 cut(s) 397
BstC8I GCNNGC 2 cut(s) 393, 430
BstDEI CTNAG 3 cut(s) 35, 144, 203
BstF5I GGATG 3 cut(s) 98, 185, 328
BstHHI GCGC 1 cut(s) 85
BstKTI GATC 1 cut(s) 41
BstMBI GATC 1 cut(s) 38
BstMWI GCNNNNNNNGC 2 cut(s) 130, 397
BstSCI CCNGG 1 cut(s) 386
BstSFI CTRYAG 1 cut(s) 362
BstV2I GAAGAC 1 cut(s) 309
BstX2I RGATCY 1 cut(s) 38
BstYI RGATCY 1 cut(s) 38
BsuRI GGCC 1 cut(s) 428
BtsCI GGATG 3 cut(s) 98, 185, 328
BveI ACCTGC 1 cut(s) 355
Cac8I GCNNGC 2 cut(s) 393, 430
CfoI GCGC 1 cut(s) 85
CseI GACGC 1 cut(s) 183
Csp6I GTAC 1 cut(s) 96
CviJI RGCY 9 cut(s) 34, 47, 71, 124, 281, 344, 381, 395, 428
CviKI_1 RGCY 9 cut(s) 34, 47, 71, 124, 281, 344, 381, 395, 428
CviQI GTAC 1 cut(s) 96
DdeI CTNAG 3 cut(s) 35, 144, 203
DpnI GATC 1 cut(s) 40
DpnII GATC 1 cut(s) 38
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
Eco147I AGGCCT 1 cut(s) 428
FaiI YATR 4 cut(s) 170, 192, 257, 347
FokI GGATG 3 cut(s) 85, 192, 335
FspBI CTAG 3 cut(s) 378, 401, 445
GlaI GCGC 1 cut(s) 84
GsuI CTGGAG 2 cut(s) 27, 107
HaeIII GGCC 1 cut(s) 428
HapII CCGG 1 cut(s) 387
HgaI GACGC 1 cut(s) 183
HhaI GCGC 1 cut(s) 85
Hin6I GCGC 1 cut(s) 83
HinP1I GCGC 1 cut(s) 83
HindIII AAGCTT 1 cut(s) 122
HinfI GANTC 1 cut(s) 404
HpaII CCGG 1 cut(s) 387
HphI GGTGA 1 cut(s) 260
Hpy188I TCNGA 2 cut(s) 339, 418
HpyAV CCTTC 3 cut(s) 142, 307, 393
HpyCH4III ACNGT 1 cut(s) 21
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 2 cut(s) 356, 364
HpyF10VI GCNNNNNNNGC 2 cut(s) 130, 397
HpyF3I CTNAG 3 cut(s) 35, 144, 203
HpySE526I ACGT 1 cut(s) 306
HspAI GCGC 1 cut(s) 83
Kzo9I GATC 1 cut(s) 38
LguI GCTCTTC 1 cut(s) 24
LmnI GCTCC 1 cut(s) 278
LpnPI CCDG 7 cut(s) 57, 71, 115, 342, 350, 400, 442
LweI GCATC 1 cut(s) 422
MaeI CTAG 3 cut(s) 378, 401, 445
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 1 cut(s) 164
MalI GATC 1 cut(s) 40
MboI GATC 1 cut(s) 38
MboII GAAGA 2 cut(s) 41, 314
MflI RGATCY 1 cut(s) 38
MluCI AATT 2 cut(s) 263, 267
MmeI TCCRAC 1 cut(s) 256
MnlI CCTC 2 cut(s) 310, 418
MseI TTAA 3 cut(s) 120, 266, 291
MslI CAYNNNNRTG 1 cut(s) 19
MspI CCGG 1 cut(s) 387
MspR9I CCNGG 1 cut(s) 388
MwoI GCNNNNNNNGC 2 cut(s) 130, 397
NciI CCSGG 1 cut(s) 388
NdeII GATC 1 cut(s) 38
NmuCI GTSAC 1 cut(s) 164
PceI AGGCCT 1 cut(s) 428
PciSI GCTCTTC 1 cut(s) 24
PfeI GAWTC 1 cut(s) 404
PshBI ATTAAT 1 cut(s) 266
PstI CTGCAG 1 cut(s) 366
PsuI RGATCY 1 cut(s) 38
RsaI GTAC 1 cut(s) 97
RsaNI GTAC 1 cut(s) 96
RseI CAYNNNNRTG 1 cut(s) 19
SapI GCTCTTC 1 cut(s) 24
SaqAI TTAA 3 cut(s) 120, 266, 291
Sau3AI GATC 1 cut(s) 38
ScrFI CCNGG 1 cut(s) 388
SfaNI GCATC 1 cut(s) 422
SfcI CTRYAG 1 cut(s) 362
SmiMI CAYNNNNRTG 1 cut(s) 19
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 2 cut(s) 263, 267
SseBI AGGCCT 1 cut(s) 428
SspMI CTAG 3 cut(s) 378, 401, 445
StuI AGGCCT 1 cut(s) 428
StyD4I CCNGG 1 cut(s) 386
TaaI ACNGT 1 cut(s) 21
TaiI ACGT 1 cut(s) 309
TasI AATT 2 cut(s) 263, 267
TatI WGTACW 1 cut(s) 95
TfiI GAWTC 1 cut(s) 404
Tru1I TTAA 3 cut(s) 120, 266, 291
Tru9I TTAA 3 cut(s) 120, 266, 291
TseFI GTSAC 1 cut(s) 164
Tsp45I GTSAC 1 cut(s) 164
TspDTI ATGAA 1 cut(s) 24
VspI ATTAAT 1 cut(s) 266
XspI CTAG 3 cut(s) 378, 401, 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.