Rh1BG001500

eukaryotic translation initiation factor isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0020266
Physical Location & Seq
Reverse (-)
213619 .. 233401
19783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG001500.1

Sequence Viewer

Length: 534 bp
ATGAATGGAGGGACGTGGTGGGCAGAGAGCAATCAACTTAGGAAAAAAACAGTCTCTCTTTTGGAGGAATATTTCAGTGTGCGAATTTTAGATGAAGCATTACTGTGTGTGGAGGAGCTAAGATCTCCAGCTTACCACCCTGAGGTTGTCAAAGAAGCTATCTCCCTTGCGCTGGAGAAAAGTACATCCTGTGTTGAACCACTTTTTAAGCTTCTCGAGCATTTGTTCTCTAAGAAGGTCATTACAGCGAGTGACATAAGGAGTGGATGTTTGCTCTATGCGTCTTTGCTGAGTGATATCGGCATTGATTATCCTAAAGCACCGAGTTATTTTGGTGAAGTTATGGGAAAATTAATTTTAGTTGGAGCTTTGGATTTTAAGGTGGTGGAAGACGTTTTGAAGGAGGTGGATGACGACTACTATCAGAAAGCCATATTCAATGCAGGACTGCAGGTTATTAGTTCTAGCCCTTCCGGGCAAGCTGTGCTGGATTCACAAGCATCTGATATTGAGGCCTGCCGGAGTCTATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05416 map03013 map05416
Pfam Domains
Protein Families
MA3 MIF4G

Protein Analysis

177

Amino Acids

19.59

Weight (kDa)

4.73

Isoelectric Point (pI)

44.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MA3 PF02847 13 - 125 6.9e-28 MA3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020266)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30680
pyrus_communis pycom15g13490
rosa_chinensis RchiOBHm_Chr5g0028791
rosa_samantha Rh1BG001500 Rh1CG009100 Rh2DG419500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 442
AcsI RAATTY 1 cut(s) 84
AfaI GTAC 1 cut(s) 184
AfiI CCNNNNNNNGG 2 cut(s) 142, 172
AgsI TTSAA 3 cut(s) 197, 400, 439
AjiI CACGTC 1 cut(s) 15
AluBI AGCT 6 cut(s) 118, 131, 158, 211, 368, 482
AluI AGCT 6 cut(s) 118, 131, 158, 211, 368, 482
Alw26I GTCTC 1 cut(s) 58
Ama87I CYCGRG 1 cut(s) 215
AoxI GGCC 1 cut(s) 513
ApoI RAATTY 1 cut(s) 84
AseI ATTAAT 1 cut(s) 353
AspLEI GCGC 1 cut(s) 172
AsuC2I CCSGG 1 cut(s) 475
AsuHPI GGTGA 1 cut(s) 347
AvaI CYCGRG 1 cut(s) 215
AxyI CCTNAGG 1 cut(s) 141
BbsI GAAGAC 1 cut(s) 396
BcnI CCSGG 1 cut(s) 475
BcoDI GTCTC 1 cut(s) 58
BfaI CTAG 2 cut(s) 465, 532
BfmI CTRYAG 1 cut(s) 449
BfuAI ACCTGC 1 cut(s) 442
BglII AGATCT 1 cut(s) 122
Bme1390I CCNGG 1 cut(s) 475
BmeT110I CYCGRG 1 cut(s) 215
BmgBI CACGTC 1 cut(s) 15
BmrFI CCNGG 1 cut(s) 475
BmsI GCATC 1 cut(s) 509
BpiI GAAGAC 1 cut(s) 396
BpmI CTGGAG 2 cut(s) 111, 194
BpuMI CCSGG 1 cut(s) 475
BsaXI ACNNNNNCTCC 2 cut(s) 253, 283
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 172
Bse21I CCTNAGG 1 cut(s) 141
BseGI GGATG 3 cut(s) 185, 272, 415
BseLI CCNNNNNNNGG 2 cut(s) 142, 172
BseMII CTCAG 2 cut(s) 132, 281
BseRI GAGGAG 1 cut(s) 128
BshFI GGCC 1 cut(s) 515
BsiHKCI CYCGRG 1 cut(s) 215
BsiSI CCGG 2 cut(s) 474, 520
BslFI GGGAC 1 cut(s) 25
BslI CCNNNNNNNGG 2 cut(s) 142, 172
BsmAI GTCTC 1 cut(s) 58
BsmFI GGGAC 1 cut(s) 25
BsnI GGCC 1 cut(s) 515
BsoBI CYCGRG 1 cut(s) 215
Bsp143I GATC 1 cut(s) 122
BspANI GGCC 1 cut(s) 515
BspCNI CTCAG 2 cut(s) 133, 282
BspMAI CTGCAG 1 cut(s) 453
BspMI ACCTGC 1 cut(s) 442
BssMI GATC 1 cut(s) 122
Bst4CI ACNGT 2 cut(s) 52, 105
BstAPI GCANNNNNTGC 1 cut(s) 484
BstC8I GCNNGC 2 cut(s) 480, 517
BstDEI CTNAG 5 cut(s) 38, 119, 141, 231, 290
BstF5I GGATG 3 cut(s) 185, 272, 415
BstHHI GCGC 1 cut(s) 172
BstKTI GATC 1 cut(s) 125
BstMAI GTCTC 1 cut(s) 58
BstMBI GATC 1 cut(s) 122
BstMWI GCNNNNNNNGC 2 cut(s) 217, 484
BstSCI CCNGG 1 cut(s) 473
BstSFI CTRYAG 1 cut(s) 449
BstV2I GAAGAC 1 cut(s) 396
BstX2I RGATCY 1 cut(s) 122
BstYI RGATCY 1 cut(s) 122
Bsu36I CCTNAGG 1 cut(s) 141
BsuRI GGCC 1 cut(s) 515
BtrI CACGTC 1 cut(s) 15
BtsCI GGATG 3 cut(s) 185, 272, 415
BtsIMutI CAGTG 1 cut(s) 82
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 2 cut(s) 480, 517
CfoI GCGC 1 cut(s) 172
CseI GACGC 1 cut(s) 270
Csp6I GTAC 1 cut(s) 183
CviJI RGCY 9 cut(s) 118, 131, 158, 211, 368, 431, 468, 482, 515
CviKI_1 RGCY 9 cut(s) 118, 131, 158, 211, 368, 431, 468, 482, 515
CviQI GTAC 1 cut(s) 183
DdeI CTNAG 5 cut(s) 38, 119, 141, 231, 290
DpnI GATC 1 cut(s) 124
DpnII GATC 1 cut(s) 122
Eco147I AGGCCT 1 cut(s) 515
Eco32I GATATC 1 cut(s) 298
Eco81I CCTNAGG 1 cut(s) 141
Eco88I CYCGRG 1 cut(s) 215
EcoRV GATATC 1 cut(s) 298
FaiI YATR 4 cut(s) 257, 279, 344, 434
FaqI GGGAC 1 cut(s) 25
FokI GGATG 3 cut(s) 172, 279, 422
FspBI CTAG 2 cut(s) 465, 532
GlaI GCGC 1 cut(s) 171
GsuI CTGGAG 2 cut(s) 111, 194
HaeIII GGCC 1 cut(s) 515
HapII CCGG 2 cut(s) 474, 520
HgaI GACGC 1 cut(s) 270
HhaI GCGC 1 cut(s) 172
Hin6I GCGC 1 cut(s) 170
HinP1I GCGC 1 cut(s) 170
HindIII AAGCTT 1 cut(s) 209
HinfI GANTC 2 cut(s) 491, 523
HpaII CCGG 2 cut(s) 474, 520
HphI GGTGA 1 cut(s) 347
Hpy188I TCNGA 2 cut(s) 426, 505
Hpy188III TCNNGA 1 cut(s) 215
HpyAV CCTTC 3 cut(s) 229, 394, 480
HpyCH4III ACNGT 2 cut(s) 52, 105
HpyCH4IV ACGT 2 cut(s) 14, 393
HpyCH4V TGCA 2 cut(s) 443, 451
HpyF10VI GCNNNNNNNGC 2 cut(s) 217, 484
HpyF3I CTNAG 5 cut(s) 38, 119, 141, 231, 290
HpySE526I ACGT 2 cut(s) 14, 393
HspAI GCGC 1 cut(s) 170
Kzo9I GATC 1 cut(s) 122
LmnI GCTCC 2 cut(s) 115, 365
LpnPI CCDG 9 cut(s) 141, 153, 158, 202, 429, 437, 473, 487, 529
LweI GCATC 1 cut(s) 509
MaeI CTAG 2 cut(s) 465, 532
MaeII ACGT 2 cut(s) 14, 393
MaeIII GTNAC 1 cut(s) 251
MalI GATC 1 cut(s) 124
MboI GATC 1 cut(s) 122
MboII GAAGA 1 cut(s) 401
MflI RGATCY 1 cut(s) 122
MluCI AATT 3 cut(s) 84, 350, 354
MlyI GAGTC 1 cut(s) 532
MmeI TCCRAC 1 cut(s) 343
MnlI CCTC 5 cut(s) 58, 106, 136, 397, 505
MseI TTAA 3 cut(s) 207, 353, 378
MslI CAYNNNNRTG 1 cut(s) 103
MspI CCGG 2 cut(s) 474, 520
MspR9I CCNGG 1 cut(s) 475
MwoI GCNNNNNNNGC 2 cut(s) 217, 484
NciI CCSGG 1 cut(s) 475
NdeII GATC 1 cut(s) 122
NmuCI GTSAC 1 cut(s) 251
PaeR7I CTCGAG 1 cut(s) 215
PceI AGGCCT 1 cut(s) 515
PfeI GAWTC 1 cut(s) 491
PleI GAGTC 1 cut(s) 531
PpsI GAGTC 1 cut(s) 531
PshBI ATTAAT 1 cut(s) 353
PstI CTGCAG 1 cut(s) 453
PsuI RGATCY 1 cut(s) 122
RsaI GTAC 1 cut(s) 184
RsaNI GTAC 1 cut(s) 183
RseI CAYNNNNRTG 1 cut(s) 103
SaqAI TTAA 3 cut(s) 207, 353, 378
Sau3AI GATC 1 cut(s) 122
SchI GAGTC 1 cut(s) 532
ScrFI CCNGG 1 cut(s) 475
SfaNI GCATC 1 cut(s) 509
SfcI CTRYAG 1 cut(s) 449
Sfr274I CTCGAG 1 cut(s) 215
SlaI CTCGAG 1 cut(s) 215
SmiMI CAYNNNNRTG 1 cut(s) 103
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 3 cut(s) 84, 350, 354
SseBI AGGCCT 1 cut(s) 515
SspI AATATT 1 cut(s) 71
SspMI CTAG 2 cut(s) 465, 532
StuI AGGCCT 1 cut(s) 515
StyD4I CCNGG 1 cut(s) 473
TaaI ACNGT 2 cut(s) 52, 105
TaiI ACGT 2 cut(s) 17, 396
TaqI TCGA 1 cut(s) 216
TasI AATT 3 cut(s) 84, 350, 354
TatI WGTACW 1 cut(s) 182
TfiI GAWTC 1 cut(s) 491
Tru1I TTAA 3 cut(s) 207, 353, 378
Tru9I TTAA 3 cut(s) 207, 353, 378
TscAI CASTG 1 cut(s) 82
TseFI GTSAC 1 cut(s) 251
Tsp45I GTSAC 1 cut(s) 251
TspDTI ATGAA 2 cut(s) 17, 108
TspRI CASTG 1 cut(s) 82
VspI ATTAAT 1 cut(s) 353
XapI RAATTY 1 cut(s) 84
XhoI CTCGAG 1 cut(s) 215
XspI CTAG 2 cut(s) 465, 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.