AT5G02570

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0021857
Physical Location & Seq
Reverse (-)
576655 .. 577176
522 bp
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UTR
Exon/CDS
Intron
AT5G02570.1

Sequence Viewer

Length: 399 bp
ATGGCGCCGAAGGCAGAGAAGAAACCGGCGGAGAAAGCACCAGCACCAAAGGCGGAGAAGAAGATCGCGAAAGAAGGAGGAACGAGCGAGATTGTCAAGAAGAAGAAGAAGACGAAGAAGAGCACAGAGACGTACAAGATCTACATTTTCAAGGTATTGAAACAGGTTCATCCAGATATTGGAATCTCTGGTAAAGCGATGGGAATCATGAACAGTTTCATCAACGACATTTTCGAGAAACTCGCTCAGGAATCGTCTCGTCTCGCTCGTTACAATAAGAAACCGACGATCACGTCTCGTGAAATCCAAACCGCCGTGAGACTTGTTCTGCCTGGTGAATTGGCTAAGCATGCCGTCTCTGAAGGAACCAAAGCTGTCACCAAATTCACAAGCTCTTAA

Protein Analysis

132

Amino Acids

14.54

Weight (kDa)

10.06

Isoelectric Point (pI)

27.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 33 - 108 9.1e-20 Core histone H2A/H2B/H3/H4 domain
Histone_ABTB PF26281 62 - 131 5.2e-08 Histone domain in ABTB family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021857)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02570
prunus_persica Prupe.1G169000_v2.0.a1
rosa_samantha Rh5BG501400
rosa_wichuraiana Rw5G044660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 292
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 1 cut(s) 179
AccII CGCG 1 cut(s) 68
AciI CCGC 3 cut(s) 29, 53, 312
AcsI RAATTY 1 cut(s) 383
AcuI CTGAAG 1 cut(s) 381
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 134
AfiI CCNNNNNNNGG 1 cut(s) 179
AgsI TTSAA 2 cut(s) 151, 160
AjiI CACGTC 1 cut(s) 294
AjnI CCWGG 1 cut(s) 331
AluBI AGCT 2 cut(s) 374, 393
AluI AGCT 2 cut(s) 374, 393
Alw21I GWGCWC 1 cut(s) 125
Alw26I GTCTC 6 cut(s) 122, 261, 266, 300, 313, 361
ApoI RAATTY 1 cut(s) 383
Asp700I GAANNNNTTC 1 cut(s) 215
AspLEI GCGC 1 cut(s) 7
AsuHPI GGTGA 2 cut(s) 347, 370
BanI GGYRCC 1 cut(s) 4
BauI CACGAG 1 cut(s) 297
BbsI GAAGAC 1 cut(s) 116
Bbv12I GWGCWC 1 cut(s) 125
BccI CCATC 1 cut(s) 193
BceAI ACGGC 2 cut(s) 299, 338
BciT130I CCWGG 1 cut(s) 333
BcoDI GTCTC 6 cut(s) 122, 261, 266, 300, 313, 361
BfoI RGCGCY 1 cut(s) 8
BglII AGATCT 1 cut(s) 138
BlpI GCTNAGC 1 cut(s) 345
Bme1390I CCNGG 1 cut(s) 333
BmgBI CACGTC 1 cut(s) 294
BmiI GGNNCC 2 cut(s) 6, 367
BmrFI CCNGG 1 cut(s) 333
BpiI GAAGAC 1 cut(s) 116
Bpu10I CCTNAGC 1 cut(s) 246
Bpu1102I GCTNAGC 1 cut(s) 345
BsaBI GATNNNNATC 1 cut(s) 203
BsaHI GRCGYC 1 cut(s) 5
Bsc4I CCNNNNNNNGG 1 cut(s) 179
Bse118I RCCGGY 1 cut(s) 25
Bse8I GATNNNNATC 1 cut(s) 203
BseBI CCWGG 1 cut(s) 333
BseGI GGATG 1 cut(s) 169
BseJI GATNNNNATC 1 cut(s) 203
BseLI CCNNNNNNNGG 1 cut(s) 179
BseMII CTCAG 1 cut(s) 260
Bsh1236I CGCG 1 cut(s) 68
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 125
BsiSI CCGG 1 cut(s) 26
BslI CCNNNNNNNGG 1 cut(s) 179
BsmAI GTCTC 6 cut(s) 122, 261, 266, 300, 313, 361
BsmBI CGTCTC 5 cut(s) 122, 261, 266, 300, 361
Bsp1286I GDGCHC 1 cut(s) 125
Bsp143I GATC 3 cut(s) 63, 138, 288
Bsp1720I GCTNAGC 1 cut(s) 345
Bsp68I TCGCGA 1 cut(s) 68
BspACI CCGC 3 cut(s) 29, 53, 312
BspCNI CTCAG 1 cut(s) 259
BspFNI CGCG 1 cut(s) 68
BspHI TCATGA 1 cut(s) 207
BspLI GGNNCC 2 cut(s) 6, 367
BspQI GCTCTTC 1 cut(s) 113
BspT107I GGYRCC 1 cut(s) 4
BsrFI RCCGGY 1 cut(s) 25
BssAI RCCGGY 1 cut(s) 25
BssMI GATC 3 cut(s) 63, 138, 288
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 297
Bst2BI CACGAG 1 cut(s) 297
Bst2UI CCWGG 1 cut(s) 333
Bst4CI ACNGT 1 cut(s) 215
Bst6I CTCTTC 1 cut(s) 113
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 1 cut(s) 351
BstDEI CTNAG 2 cut(s) 246, 345
BstF5I GGATG 1 cut(s) 169
BstFNI CGCG 1 cut(s) 68
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 3 cut(s) 66, 141, 291
BstMAI GTCTC 6 cut(s) 122, 261, 266, 300, 313, 361
BstMBI GATC 3 cut(s) 63, 138, 288
BstMWI GCNNNNNNNGC 4 cut(s) 11, 35, 50, 350
BstNI CCWGG 1 cut(s) 333
BstNSI RCATGY 1 cut(s) 353
BstSCI CCNGG 1 cut(s) 331
BstUI CGCG 1 cut(s) 68
BstV2I GAAGAC 1 cut(s) 116
BstX2I RGATCY 1 cut(s) 138
BstYI RGATCY 1 cut(s) 138
BtgZI GCGATG 1 cut(s) 212
BtrI CACGTC 1 cut(s) 294
BtsCI GGATG 1 cut(s) 169
BtuMI TCGCGA 1 cut(s) 68
Cac8I GCNNGC 1 cut(s) 351
CciI TCATGA 1 cut(s) 207
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 25
Csp6I GTAC 1 cut(s) 133
CviAII CATG 2 cut(s) 208, 350
CviJI RGCY 3 cut(s) 344, 374, 393
CviKI_1 RGCY 3 cut(s) 344, 374, 393
CviQI GTAC 1 cut(s) 133
DdeI CTNAG 2 cut(s) 246, 345
DinI GGCGCC 1 cut(s) 6
DpnI GATC 3 cut(s) 65, 140, 290
DpnII GATC 3 cut(s) 63, 138, 288
DrdI GACNNNNNNGTC 1 cut(s) 292
DseDI GACNNNNNNGTC 1 cut(s) 292
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
EciI GGCGGA 2 cut(s) 44, 68
Eco57I CTGAAG 1 cut(s) 381
EcoRII CCWGG 1 cut(s) 331
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
Esp3I CGTCTC 5 cut(s) 122, 261, 266, 300, 361
FaeI CATG 2 cut(s) 211, 353
FaiI YATR 2 cut(s) 209, 351
FatI CATG 2 cut(s) 207, 349
FokI GGATG 1 cut(s) 156
GlaI GCGC 1 cut(s) 6
HaeII RGCGCY 1 cut(s) 8
HapII CCGG 1 cut(s) 26
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 211, 353
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 3 cut(s) 183, 204, 251
HpaII CCGG 1 cut(s) 26
HphI GGTGA 2 cut(s) 347, 370
Hpy188I TCNGA 1 cut(s) 361
Hpy188III TCNNGA 7 cut(s) 67, 97, 173, 208, 235, 248, 299
Hpy99I CGWCG 1 cut(s) 289
HpyAV CCTTC 3 cut(s) 4, 68, 356
HpyCH4III ACNGT 1 cut(s) 215
HpyCH4IV ACGT 2 cut(s) 131, 293
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 35, 50, 350
HpyF3I CTNAG 2 cut(s) 246, 345
HpySE526I ACGT 2 cut(s) 131, 293
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 211, 353
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 3 cut(s) 63, 138, 288
LguI GCTCTTC 1 cut(s) 113
LpnPI CCDG 8 cut(s) 39, 54, 149, 174, 186, 233, 318, 345
MaeII ACGT 2 cut(s) 131, 293
MaeIII GTNAC 2 cut(s) 269, 376
MalI GATC 3 cut(s) 65, 140, 290
MboI GATC 3 cut(s) 63, 138, 288
MboII GAAGA 9 cut(s) 31, 70, 73, 112, 115, 118, 121, 127, 130
MflI RGATCY 1 cut(s) 138
MhlI GDGCHC 1 cut(s) 125
MluCI AATT 2 cut(s) 338, 383
Mly113I GGCGCC 1 cut(s) 5
MnlI CCTC 1 cut(s) 71
MroXI GAANNNNTTC 1 cut(s) 215
MseI TTAA 1 cut(s) 397
MspI CCGG 1 cut(s) 26
MspR9I CCNGG 1 cut(s) 333
MvaI CCWGG 1 cut(s) 333
MvnI CGCG 1 cut(s) 68
MwoI GCNNNNNNNGC 4 cut(s) 11, 35, 50, 350
NarI GGCGCC 1 cut(s) 5
NdeII GATC 3 cut(s) 63, 138, 288
NlaIII CATG 2 cut(s) 211, 353
NlaIV GGNNCC 2 cut(s) 6, 367
NmuCI GTSAC 1 cut(s) 376
NruI TCGCGA 1 cut(s) 68
NspI RCATGY 1 cut(s) 353
PaeI GCATGC 1 cut(s) 353
PagI TCATGA 1 cut(s) 207
PciSI GCTCTTC 1 cut(s) 113
PcsI WCGNNNNNNNCGW 2 cut(s) 231, 265
PdmI GAANNNNTTC 1 cut(s) 215
PfeI GAWTC 3 cut(s) 183, 204, 251
PflMI CCANNNNNTGG 1 cut(s) 179
PluTI GGCGCC 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 331
PspGI CCWGG 1 cut(s) 331
PspN4I GGNNCC 2 cut(s) 6, 367
PsuI RGATCY 1 cut(s) 138
RruI TCGCGA 1 cut(s) 68
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
SapI GCTCTTC 1 cut(s) 113
SaqAI TTAA 1 cut(s) 397
Sau3AI GATC 3 cut(s) 63, 138, 288
ScrFI CCNGG 1 cut(s) 333
SduI GDGCHC 1 cut(s) 125
SetI ASST 6 cut(s) 134, 156, 168, 296, 376, 395
SfoI GGCGCC 1 cut(s) 6
SphI GCATGC 1 cut(s) 353
Sse9I AATT 2 cut(s) 338, 383
SsiI CCGC 3 cut(s) 29, 53, 312
SspDI GGCGCC 1 cut(s) 4
StyD4I CCNGG 1 cut(s) 331
TaaI ACNGT 1 cut(s) 215
TaiI ACGT 2 cut(s) 134, 296
TaqI TCGA 1 cut(s) 234
TasI AATT 2 cut(s) 338, 383
TfiI GAWTC 3 cut(s) 183, 204, 251
Tru1I TTAA 1 cut(s) 397
Tru9I TTAA 1 cut(s) 397
TseFI GTSAC 1 cut(s) 376
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 3 cut(s) 158, 208, 224
Van91I CCANNNNNTGG 1 cut(s) 179
XapI RAATTY 1 cut(s) 383
XceI RCATGY 1 cut(s) 353
XmnI GAANNNNTTC 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.