AT5G08260

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0020147
Physical Location & Seq
Forward (+)
2657054 .. 2661437
4384 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G08260.1

Sequence Viewer

Length: 1443 bp
ATGAAGAAGAACGCACTTTGGCTACTGTGCATTCTGGTGTTACCGGCGATAGCCTGTGGTAGGAAACCGGAGAAGAAGGTAACAATTTCATCCTCTGGGAGGAAGGAAGATGACCTTGTAACCGGTTTGCCCGGCCAACCACCGGTTAACTTCAAACACTACGCCGGTTACGTGAATCTGGGGCCTGAACAGAAGCAAAAGGCACTTTTCTATTGGTTCTTCGAAGCTCAACAAAACTCTTCTCGTCGTCCTCTCGTCCTTTGGCTCAATGGAGGACCGGGTTGCTCATCAATAGCCTATGGAGCTGCACAAGAACTAGGCCCTTTTCTTGTTCACGACAACGGTGGCAAACTTACCTACAATCATTTCTCTTGGAACAAAGAGGCCAATATGTTGTTCTTGGAAGCGCCGGTTGGGGTTGGGTTCTCGTATACCAATAACTCAATGGATCTTCAAAAGCTTGGAGATGAAGTTACTGCATCGGACTCTCTTGCTTTCCTGATCAACTGGTTCATGAAATTCCCCGAGTTCCGATCCAGCGAGTTCTACATCTCTGGCGAGAGTTACGCCGGTCACTATGTTCCTCAGCTCGCGGAGGTAATTTACGACAGGAACAAGAAGGTTACGAAAGACTCTAGCATTAATCTCAAGGGATTCATGATAGGGAACGCGGTGATCAATGAGGCAACAGACATGGCAGGACTAGTGGACTACGCATGGAGCCACGCTATAATATCGGACGAGGTCCACACCAGCATCCATGGCTCGTGCAGTTTTGAGGAAGATACCACCAACAAGACCGAACAATGCTACAACAATTTCAAAGGGTTCATGGATGCTTACAATGATATCGATATATACAGTATCTACACTCCCGTTTGCCTCTCCTCTTTGTTGTCATCTTCTCCAAGAAAACCTAAAATCGTCGTGTCTCCTCGTCTCCTTACTTTTGACGATTTGTGGGACAAGTTTCCGGCCGGGTACGATCCATGCACTGAAAGTTATGCCGAGAATTACTTTAACCGGAAAGATGTGCAGGTTGCACTCCATGCGAACGTCACCAACCTTCCTTATCCGTACTCTCCTTGCAGCGGAGTAATAAAGAGATGGAGTGATGCTCCATCCACCATGATTCCCATCATACAAAAGCTTTTGACGGGTGGCCTCCGTATCTGGATCTATAGTGGAGATACGGATGGGAGAGTTCCGGTAACATCTACACGTTATAGCATAAAAAAGATGGGACTTAAGGTCGAGTCACCGTGGAGGTCATGGTTCCACAAGAGTCAAGTAGCTGGATGGGTTGAGACTTATGCCGGTGGACTAAATTTTGTCACGGTGAGAGGAGCCGGTCACCAGGTTCCGGCCTTGGCTCCGGCGCAATCTCTCACATTATTCTCTCACTTCATTTCTTCTGTCCCCTTGCCTTCAAAACGGTTCTGA

Protein Analysis

480

Amino Acids

53.61

Weight (kDa)

7.98

Isoelectric Point (pI)

48.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 44 - 471 2.9e-146 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020147)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08260
malus_domestica MD13G1004500.v1.1 MD16G1003100.v1.1
prunus_persica Prupe.1G348100_v2.0.a1
pyrus_communis pycom13g00390 pycom16g00300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1027
AccI GTMKAC 1 cut(s) 431
AccII CGCG 2 cut(s) 593, 671
AciI CCGC 3 cut(s) 593, 671, 1092
AclWI GGATC 4 cut(s) 456, 528, 980, 1184
AcoI YGGCCR 2 cut(s) 133, 975
AcsI RAATTY 2 cut(s) 518, 1327
AfaI GTAC 2 cut(s) 983, 1079
AfiI CCNNNNNNNGG 5 cut(s) 60, 99, 142, 1091, 1363
AflII CTTAAG 1 cut(s) 1247
AflIII ACRYGT 1 cut(s) 1220
AgeI ACCGGT 2 cut(s) 122, 142
AgsI TTSAA 4 cut(s) 154, 455, 823, 1431
AhdI GACNNNNNGTC 1 cut(s) 1250
AhlI ACTAGT 1 cut(s) 703
AjnI CCWGG 1 cut(s) 1356
AjuI GAANNNNNNNTTGG 4 cut(s) 380, 396, 412, 428
AluBI AGCT 6 cut(s) 227, 305, 460, 589, 1150, 1295
AluI AGCT 6 cut(s) 227, 305, 460, 589, 1150, 1295
Alw26I GTCTC 3 cut(s) 936, 944, 1301
AlwI GGATC 4 cut(s) 456, 528, 980, 1184
Ama87I CYCGRG 1 cut(s) 524
AoxI GGCC 7 cut(s) 133, 182, 319, 384, 975, 1162, 1365
ApeKI GCWGC 2 cut(s) 305, 1089
ApoI RAATTY 2 cut(s) 518, 1327
AseI ATTAAT 1 cut(s) 642
AsiGI ACCGGT 2 cut(s) 122, 142
AspLEI GCGC 2 cut(s) 409, 1381
AspS9I GGNCC 4 cut(s) 182, 275, 320, 745
AsuC2I CCSGG 3 cut(s) 132, 279, 979
AsuHPI GGTGA 5 cut(s) 685, 1051, 1251, 1346, 1351
AsuII TTCGAA 1 cut(s) 222
AvaI CYCGRG 1 cut(s) 524
AvaII GGWCC 2 cut(s) 275, 745
BauI CACGAG 1 cut(s) 766
BbvCI CCTCAGC 1 cut(s) 585
BbvI GCAGC 2 cut(s) 292, 1101
BccI CCATC 6 cut(s) 1101, 1129, 1145, 1190, 1234, 1293
BciT130I CCWGG 1 cut(s) 1358
BclI TGATCA 2 cut(s) 501, 675
BcnI CCSGG 3 cut(s) 132, 279, 979
BcoDI GTCTC 3 cut(s) 936, 944, 1301
BcuI ACTAGT 1 cut(s) 703
BfaI CTAG 3 cut(s) 317, 636, 704
BfmI CTRYAG 1 cut(s) 1180
BfoI RGCGCY 1 cut(s) 410
BfrI CTTAAG 1 cut(s) 1247
BfuAI ACCTGC 1 cut(s) 1027
BisI GCNGC 2 cut(s) 306, 1090
BlsI GCNGC 2 cut(s) 307, 1091
Bme1390I CCNGG 4 cut(s) 132, 279, 979, 1358
Bme18I GGWCC 2 cut(s) 275, 745
BmeRI GACNNNNNGTC 1 cut(s) 1250
BmeT110I CYCGRG 1 cut(s) 524
BmgT120I GGNCC 4 cut(s) 182, 275, 320, 745
BmiI GGNNCC 6 cut(s) 183, 722, 1277, 1348, 1362, 1374
BmrFI CCNGG 4 cut(s) 132, 279, 979, 1358
BmsI GCATC 4 cut(s) 488, 765, 826, 1105
BplI GAGNNNNNCTC 2 cut(s) 1102, 1134
Bpu10I CCTNAGC 1 cut(s) 585
Bpu14I TTCGAA 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 632
BpuMI CCSGG 3 cut(s) 132, 279, 979
Bsa29I ATCGAT 1 cut(s) 852
BsaAI YACGTR 1 cut(s) 172
BsaBI GATNNNNATC 1 cut(s) 1136
BsaJI CCNNGG 3 cut(s) 760, 1262, 1368
BsaWI WCCGGW 5 cut(s) 67, 122, 142, 1023, 1207
BsaXI ACNNNNNCTCC 2 cut(s) 456, 486
Bsc4I CCNNNNNNNGG 5 cut(s) 60, 99, 142, 1091, 1363
Bse118I RCCGGY 8 cut(s) 43, 122, 142, 164, 409, 569, 1316, 1349
Bse1I ACTGG 1 cut(s) 512
Bse8I GATNNNNATC 1 cut(s) 1136
BseBI CCWGG 1 cut(s) 1358
BseCI ATCGAT 1 cut(s) 852
BseDI CCNNGG 3 cut(s) 760, 1262, 1368
BseGI GGATG 6 cut(s) 89, 756, 841, 1121, 1201, 1304
BseJI GATNNNNATC 1 cut(s) 1136
BseLI CCNNNNNNNGG 5 cut(s) 60, 99, 142, 1091, 1363
BseMII CTCAG 1 cut(s) 599
BseNI ACTGG 1 cut(s) 512
BseRI GAGGAG 3 cut(s) 877, 924, 1359
BseX3I CGGCCG 1 cut(s) 975
BseXI GCAGC 2 cut(s) 292, 1101
BsgI GTGCAG 3 cut(s) 291, 790, 1055
Bsh1236I CGCG 2 cut(s) 593, 671
Bsh1285I CGRYCG 1 cut(s) 978
BshFI GGCC 7 cut(s) 135, 184, 321, 386, 977, 1164, 1367
BshTI ACCGGT 2 cut(s) 122, 142
BshVI ATCGAT 1 cut(s) 852
BsiEI CGRYCG 1 cut(s) 978
BsiHKCI CYCGRG 1 cut(s) 524
BslFI GGGAC 3 cut(s) 977, 1257, 1403
BslI CCNNNNNNNGG 5 cut(s) 60, 99, 142, 1091, 1363
BsmAI GTCTC 3 cut(s) 936, 944, 1301
BsmBI CGTCTC 1 cut(s) 944
BsmFI GGGAC 3 cut(s) 977, 1257, 1403
BsmI GAATGC 1 cut(s) 30
BsnI GGCC 7 cut(s) 135, 184, 321, 386, 977, 1164, 1367
BsoBI CYCGRG 1 cut(s) 524
Bsp119I TTCGAA 1 cut(s) 222
Bsp143I GATC 6 cut(s) 448, 501, 533, 675, 985, 1176
Bsp19I CCATGG 1 cut(s) 760
BspACI CCGC 3 cut(s) 593, 671, 1092
BspANI GGCC 7 cut(s) 135, 184, 321, 386, 977, 1164, 1367
BspCNI CTCAG 1 cut(s) 598
BspDI ATCGAT 1 cut(s) 852
BspFNI CGCG 2 cut(s) 593, 671
BspHI TCATGA 2 cut(s) 513, 657
BspLI GGNNCC 6 cut(s) 183, 722, 1277, 1348, 1362, 1374
BspMI ACCTGC 1 cut(s) 1027
BspPI GGATC 4 cut(s) 456, 528, 980, 1184
BspT104I TTCGAA 1 cut(s) 222
BspTI CTTAAG 1 cut(s) 1247
BsrFI RCCGGY 8 cut(s) 43, 122, 142, 164, 409, 569, 1316, 1349
BsrI ACTGG 1 cut(s) 512
BssAI RCCGGY 8 cut(s) 43, 122, 142, 164, 409, 569, 1316, 1349
BssECI CCNNGG 3 cut(s) 760, 1262, 1368
BssMI GATC 6 cut(s) 448, 501, 533, 675, 985, 1176
BssNAI GTATAC 1 cut(s) 432
BssSI CACGAG 1 cut(s) 766
BssT1I CCWWGG 2 cut(s) 760, 1368
Bst1107I GTATAC 1 cut(s) 432
Bst2BI CACGAG 1 cut(s) 766
Bst2UI CCWGG 1 cut(s) 1358
Bst4CI ACNGT 6 cut(s) 27, 344, 863, 1263, 1339, 1437
Bst6I CTCTTC 1 cut(s) 244
BstAFI CTTAAG 1 cut(s) 1247
BstAPI GCANNNNNTGC 1 cut(s) 1049
BstBAI YACGTR 1 cut(s) 172
BstBI TTCGAA 1 cut(s) 222
BstC8I GCNNGC 1 cut(s) 591
BstDEI CTNAG 1 cut(s) 585
BstDSI CCRYGG 2 cut(s) 760, 1262
BstEII GGTNACC 1 cut(s) 1352
BstENI CCTNNNNNAGG 2 cut(s) 58, 97
BstF5I GGATG 6 cut(s) 89, 756, 841, 1121, 1201, 1304
BstFNI CGCG 2 cut(s) 593, 671
BstH2I RGCGCY 1 cut(s) 410
BstHHI GCGC 2 cut(s) 409, 1381
BstKTI GATC 6 cut(s) 451, 504, 536, 678, 988, 1179
BstMAI GTCTC 3 cut(s) 936, 944, 1301
BstMBI GATC 6 cut(s) 448, 501, 533, 675, 985, 1176
BstMCI CGRYCG 1 cut(s) 978
BstMWI GCNNNNNNNGC 3 cut(s) 302, 762, 1049
BstNI CCWGG 1 cut(s) 1358
BstPI GGTNACC 1 cut(s) 1352
BstSCI CCNGG 4 cut(s) 130, 277, 977, 1356
BstSFI CTRYAG 1 cut(s) 1180
BstUI CGCG 2 cut(s) 593, 671
BstV1I GCAGC 2 cut(s) 292, 1101
BstX2I RGATCY 2 cut(s) 448, 1176
BstYI RGATCY 2 cut(s) 448, 1176
BstZ17I GTATAC 1 cut(s) 432
BstZI CGGCCG 1 cut(s) 975
Bsu15I ATCGAT 1 cut(s) 852
BsuRI GGCC 7 cut(s) 135, 184, 321, 386, 977, 1164, 1367
BsuTUI ATCGAT 1 cut(s) 852
BtgI CCRYGG 2 cut(s) 760, 1262
BtsCI GGATG 6 cut(s) 89, 756, 841, 1121, 1201, 1304
BtsIMutI CAGTG 1 cut(s) 993
BveI ACCTGC 1 cut(s) 1027
Cac8I GCNNGC 1 cut(s) 591
CciI TCATGA 2 cut(s) 513, 657
CfoI GCGC 2 cut(s) 409, 1381
Cfr10I RCCGGY 8 cut(s) 43, 122, 142, 164, 409, 569, 1316, 1349
Cfr13I GGNCC 4 cut(s) 182, 275, 320, 745
ClaI ATCGAT 1 cut(s) 852
CsiI ACCWGGT 1 cut(s) 1356
Csp6I GTAC 2 cut(s) 982, 1078
CspAI ACCGGT 2 cut(s) 122, 142
CviQI GTAC 2 cut(s) 982, 1078
DdeI CTNAG 1 cut(s) 585
DpnI GATC 6 cut(s) 450, 503, 535, 677, 987, 1178
DpnII GATC 6 cut(s) 448, 501, 533, 675, 985, 1176
DriI GACNNNNNGTC 1 cut(s) 1250
EaeI YGGCCR 2 cut(s) 133, 975
EagI CGGCCG 1 cut(s) 975
Eam1104I CTCTTC 1 cut(s) 244
Eam1105I GACNNNNNGTC 1 cut(s) 1250
EarI CTCTTC 1 cut(s) 244
EclXI CGGCCG 1 cut(s) 975
Eco130I CCWWGG 2 cut(s) 760, 1368
Eco32I GATATC 1 cut(s) 850
Eco47I GGWCC 2 cut(s) 275, 745
Eco52I CGGCCG 1 cut(s) 975
Eco88I CYCGRG 1 cut(s) 524
Eco91I GGTNACC 1 cut(s) 1352
EcoNI CCTNNNNNAGG 2 cut(s) 58, 97
EcoO109I RGGNCCY 2 cut(s) 182, 320
EcoO65I GGTNACC 1 cut(s) 1352
EcoRII CCWGG 1 cut(s) 1356
EcoRV GATATC 1 cut(s) 850
EcoT14I CCWWGG 2 cut(s) 760, 1368
ErhI CCWWGG 2 cut(s) 760, 1368
Esp3I CGTCTC 1 cut(s) 944
FalI AAGNNNNNCTT 2 cut(s) 99, 131
FaqI GGGAC 3 cut(s) 977, 1257, 1403
FbaI TGATCA 2 cut(s) 501, 675
FblI GTMKAC 1 cut(s) 431
Fnu4HI GCNGC 2 cut(s) 306, 1090
FokI GGATG 6 cut(s) 76, 743, 848, 1108, 1208, 1311
Fsp4HI GCNGC 2 cut(s) 306, 1090
FspBI CTAG 3 cut(s) 317, 636, 704
GlaI GCGC 2 cut(s) 408, 1380
GluI GCNGC 2 cut(s) 306, 1090
HaeII RGCGCY 1 cut(s) 410
HaeIII GGCC 7 cut(s) 135, 184, 321, 386, 977, 1164, 1367
HhaI GCGC 2 cut(s) 409, 1381
Hin6I GCGC 2 cut(s) 407, 1379
HinP1I GCGC 2 cut(s) 407, 1379
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HindIII AAGCTT 2 cut(s) 458, 1148
HinfI GANTC 7 cut(s) 175, 485, 632, 654, 1132, 1256, 1285
HpaI GTTAAC 1 cut(s) 148
HphI GGTGA 5 cut(s) 685, 1051, 1251, 1346, 1351
Hpy166II GTNNAC 6 cut(s) 148, 334, 432, 709, 748, 1322
Hpy188I TCNGA 4 cut(s) 484, 533, 739, 1442
Hpy188III TCNNGA 5 cut(s) 335, 499, 514, 658, 1174
Hpy8I GTNNAC 6 cut(s) 148, 334, 432, 709, 748, 1322
Hpy99I CGWCG 2 cut(s) 249, 929
HpyAV CCTTC 5 cut(s) 70, 97, 613, 1076, 1437
HpyCH4III ACNGT 6 cut(s) 27, 344, 863, 1263, 1339, 1437
HpyCH4IV ACGT 3 cut(s) 171, 1056, 1222
HpyCH4V TGCA 8 cut(s) 30, 308, 479, 771, 993, 1036, 1043, 1089
HpyF10VI GCNNNNNNNGC 3 cut(s) 302, 762, 1049
HpyF3I CTNAG 1 cut(s) 585
HpySE526I ACGT 3 cut(s) 171, 1056, 1222
HspAI GCGC 2 cut(s) 407, 1379
Ksp22I TGATCA 2 cut(s) 501, 675
KspAI GTTAAC 1 cut(s) 148
Kzo9I GATC 6 cut(s) 448, 501, 533, 675, 985, 1176
LmnI GCTCC 5 cut(s) 302, 720, 1123, 1346, 1378
Lsp1109I GCAGC 2 cut(s) 292, 1101
LweI GCATC 4 cut(s) 488, 765, 826, 1105
MabI ACCWGGT 1 cut(s) 1356
MaeI CTAG 3 cut(s) 317, 636, 704
MaeII ACGT 3 cut(s) 171, 1056, 1222
MalI GATC 6 cut(s) 450, 503, 535, 677, 987, 1178
MboI GATC 6 cut(s) 448, 501, 533, 675, 985, 1176
MflI RGATCY 2 cut(s) 448, 1176
MluCI AATT 6 cut(s) 84, 518, 600, 817, 1012, 1327
MlyI GAGTC 4 cut(s) 479, 626, 1265, 1294
MseI TTAA 4 cut(s) 147, 642, 1020, 1248
MslI CAYNNNNRTG 1 cut(s) 35
MspA1I CMGCKG 1 cut(s) 1092
MspCI CTTAAG 1 cut(s) 1247
MspR9I CCNGG 4 cut(s) 132, 279, 979, 1358
Mva1269I GAATGC 1 cut(s) 30
MvaI CCWGG 1 cut(s) 1358
MvnI CGCG 2 cut(s) 593, 671
MwoI GCNNNNNNNGC 3 cut(s) 302, 762, 1049
NciI CCSGG 3 cut(s) 132, 279, 979
NcoI CCATGG 1 cut(s) 760
NdeII GATC 6 cut(s) 448, 501, 533, 675, 985, 1176
NlaIV GGNNCC 6 cut(s) 183, 722, 1277, 1348, 1362, 1374
NmeAIII GCCGAG 1 cut(s) 1033
NmuCI GTSAC 5 cut(s) 572, 1057, 1257, 1333, 1352
NspV TTCGAA 1 cut(s) 222
PagI TCATGA 2 cut(s) 513, 657
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PctI GAATGC 1 cut(s) 30
PfeI GAWTC 3 cut(s) 175, 654, 1132
PflFI GACNNNGTC 1 cut(s) 743
PinAI ACCGGT 2 cut(s) 122, 142
PkrI GCNGC 2 cut(s) 307, 1091
PleI GAGTC 4 cut(s) 479, 626, 1264, 1293
PpsI GAGTC 4 cut(s) 479, 626, 1264, 1293
Ppu21I YACGTR 1 cut(s) 172
PshBI ATTAAT 1 cut(s) 642
Psp6I CCWGG 1 cut(s) 1356
PspEI GGTNACC 1 cut(s) 1352
PspGI CCWGG 1 cut(s) 1356
PspN4I GGNNCC 6 cut(s) 183, 722, 1277, 1348, 1362, 1374
PspPI GGNCC 4 cut(s) 182, 275, 320, 745
PsuI RGATCY 2 cut(s) 448, 1176
PsyI GACNNNGTC 1 cut(s) 743
RsaI GTAC 2 cut(s) 983, 1079
RsaNI GTAC 2 cut(s) 982, 1078
RseI CAYNNNNRTG 1 cut(s) 35
SaqAI TTAA 4 cut(s) 147, 642, 1020, 1248
SatI GCNGC 2 cut(s) 306, 1090
Sau3AI GATC 6 cut(s) 448, 501, 533, 675, 985, 1176
Sau96I GGNCC 4 cut(s) 182, 275, 320, 745
SchI GAGTC 4 cut(s) 479, 626, 1265, 1294
ScrFI CCNGG 4 cut(s) 132, 279, 979, 1358
SexAI ACCWGGT 1 cut(s) 1356
SfaNI GCATC 4 cut(s) 488, 765, 826, 1105
SfcI CTRYAG 1 cut(s) 1180
SfuI TTCGAA 1 cut(s) 222
SinI GGWCC 2 cut(s) 275, 745
SmiMI CAYNNNNRTG 1 cut(s) 35
SmlI CTYRAG 2 cut(s) 647, 1247
SmoI CTYRAG 2 cut(s) 647, 1247
SpeI ACTAGT 1 cut(s) 703
Sse9I AATT 6 cut(s) 84, 518, 600, 817, 1012, 1327
SsiI CCGC 3 cut(s) 593, 671, 1092
SspMI CTAG 3 cut(s) 317, 636, 704
StyD4I CCNGG 4 cut(s) 130, 277, 977, 1356
StyI CCWWGG 2 cut(s) 760, 1368
TaaI ACNGT 6 cut(s) 27, 344, 863, 1263, 1339, 1437
TaiI ACGT 3 cut(s) 174, 1059, 1225
TaqI TCGA 3 cut(s) 222, 852, 1254
TaqII GACCGA 1 cut(s) 815
TasI AATT 6 cut(s) 84, 518, 600, 817, 1012, 1327
TfiI GAWTC 3 cut(s) 175, 654, 1132
Tru1I TTAA 4 cut(s) 147, 642, 1020, 1248
Tru9I TTAA 4 cut(s) 147, 642, 1020, 1248
TscAI CASTG 1 cut(s) 1000
TseFI GTSAC 5 cut(s) 572, 1057, 1257, 1333, 1352
TseI GCWGC 2 cut(s) 305, 1089
Tsp45I GTSAC 5 cut(s) 572, 1057, 1257, 1333, 1352
TspDTI ATGAA 8 cut(s) 17, 78, 483, 502, 530, 646, 820, 1396
TspGWI ACGGA 3 cut(s) 1065, 1157, 1208
TspRI CASTG 1 cut(s) 1000
Tth111I GACNNNGTC 1 cut(s) 743
Vha464I CTTAAG 1 cut(s) 1247
VpaK11BI GGWCC 2 cut(s) 275, 745
VspI ATTAAT 1 cut(s) 642
XagI CCTNNNNNAGG 2 cut(s) 58, 97
XapI RAATTY 2 cut(s) 518, 1327
XcmI CCANNNNNNNNNTGG 1 cut(s) 442
XmiI GTMKAC 1 cut(s) 431
XspI CTAG 3 cut(s) 317, 636, 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.