Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0020147

Physical Location & Seq

Genomic Coordinates Forward (+)

187523 .. 193014

Gene Structure 5492 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1003100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1500 bp

ATGGCTCTTAATCCTAATTTGTTGAACATAATTATGATATGGTGTCTAGTACTACTCTTTCCTGTGATCAATGCCGTGAAACATGAAGAATTTGAAGTGCAGAGGCTACTGGGAGAAGGTGACAGGGTCACCAACTTGCCCGGACAGCCGCCGGTGTCATTCCAGCATTACGCCGGCTACGTCCGACTTCCCAATTCGGGGAAGGCCTTGTTCTACTGGTTTTTTCAAGCCCAAGAGAATGCATCTCGAAAACCCCTCGTCCTTTGGCTTAATGGAGGACCTGGGTGCTCATCTGTAGCTTATGGAGCTGCGCAAGAACTGGGACCCTTTCTTGTTCGAAGCAATGGAACCCTAATTCTTAACGATTTCTCCTGGAATAAAGTTGCCAATGTGCTGTTCCTGGAGTCACCAGTTGGAGTAGGGTTTTCTTACACAAACAACTCCAAGGATCTGCGTACTCTGGGCGATCGAATCACAGCAGCTGATTCGTTTTCTTTCCTGGCGGAATGGTTTAAGAGGTTCCCAACCTTCAAATCCCACGATTTCTACATTTCTGGGGAGAGCTACGCTGGCCATTACGTCCCCCAGCTTGCCGACCTCATCTACGACCGAAACAATCGAACCGCCAGATCATCATCCCCATCAGACTCCTCGTCTTTCATAAATCTCAAAGGTTTCATGATTGGGAATGCTGTGATCAATGGACCGACAGACAACAGTGGGATGTTTGATTACGCATGGAGTCATGCCATAATCTCGGACACGCTATACGACAATTTAGTCAAGAAATGTGATGTCGTACACGATGACAACCAAACAGAAGAGTGCAATGATCACATTCGAGCCTTCTTACAAAATTATTCTCAAATCGACATCTACGGTATTTATGCACCCCTCTGCCTCACTGATCCATCTCCATCTACTACTACTTCTCCCAAACCAACAAGGATTAATAATTTCATGAAAAATGTTGCACCTCGGCTGCTTACTCAACATGAGCTTTGGCAGAGGCTACCATCAGGGTATGATCCCTGCACAGAGGATTATGTTGAGCAATATTTCAACAGAAAGGATGTTCAGAAGGCCCTACATGCCAATGCTACTAAACTTTCCTATCCTTACTCCCCGTGCAGTGGCGTCATAAAGGAATGGAAGGACTCGCCTGACACTGTACTGCCAATCATTCAGAAGCTCTTGAAAGCTGGCTTGCGTATTTGGATTTATAGCGGTGACACGGATGGGAGGGTGCCGGTAACATCAACGAGGTACAGCATAAAGAAGATGGGTTTAAGAGTAAAGCAACCATGGAAAGCATGGTTTGATAAGGGTCAAGTGGCCGGGTGGGCGGAGACGTACGAGGGCGGCCTGACATTTGCGACGGTGAGGGGCGCAGGGCACCAAGTCCCGGTGTTTGCACCTCGTCAGTCACTTTCTCTATTTACCCATTTCCTCTATCCCAATCCCAACACTACCCACGGCCTCCCTTCGTCTCGCATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004180 GO:0004185 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0009056 GO:0009057 GO:0009987 GO:0016787 GO:0017171 GO:0019538 GO:0030163 GO:0043170 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0070008 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_S10

Protein Analysis

500

Amino Acids

56.12

Weight (kDa)

8.3

Isoelectric Point (pI)

44.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S10	PF00450	47 - 484	3.2e-140	Serine carboxypeptidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0020147)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08260
malus_domestica	MD13G1004500.v1.1 MD16G1003100.v1.1
prunus_persica	Prupe.1G348100_v2.0.a1
pyrus_communis	pycom13g00390 pycom16g00300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	779
Acc16I	TGCGCA	1 cut(s)	312
AccB1I	GGYRCC	2 cut(s)	1246, 1395
AciI	CCGC	6 cut(s)	149, 503, 624, 1227, 1346, 1362
AclWI	GGATC	3 cut(s)	456, 902, 1022
AcoI	YGGCCR	2 cut(s)	571, 1335
AcsI	RAATTY	1 cut(s)	89
AcyI	GRCGYC	1 cut(s)	1137
AfaI	GTAC	6 cut(s)	51, 457, 801, 1173, 1268, 1355
AfiI	CCNNNNNNNGG	4 cut(s)	197, 198, 1133, 1405
AgsI	TTSAA	6 cut(s)	25, 95, 227, 532, 1063, 1198
AhdI	GACNNNNNGTC	1 cut(s)	652
AjnI	CCWGG	4 cut(s)	280, 371, 399, 498
AjuI	GAANNNNNNNTTGG	2 cut(s)	380, 412
AluBI	AGCT	8 cut(s)	299, 308, 482, 564, 589, 1000, 1192, 1202
AluI	AGCT	8 cut(s)	299, 308, 482, 564, 589, 1000, 1192, 1202
Alw21I	GWGCWC	1 cut(s)	290
Alw26I	GTCTC	2 cut(s)	1343, 1494
AlwI	GGATC	3 cut(s)	456, 902, 1022
AlwNI	CAGNNNCTG	1 cut(s)	482
AoxI	GGCC	6 cut(s)	204, 571, 1083, 1335, 1363, 1477
ApeKI	GCWGC	3 cut(s)	308, 479, 982
ApoI	RAATTY	1 cut(s)	89
AseI	ATTAAT	1 cut(s)	951
AspLEI	GCGC	2 cut(s)	313, 1391
AspS9I	GGNCC	4 cut(s)	278, 323, 704, 1084
AsuC2I	CCSGG	3 cut(s)	141, 1339, 1406
AsuHPI	GGTGA	5 cut(s)	121, 131, 399, 1241, 1393
AsuII	TTCGAA	1 cut(s)	337
AvaII	GGWCC	3 cut(s)	278, 323, 704
BaeGI	GKGCMC	1 cut(s)	1398
BalI	TGGCCA	1 cut(s)	573
BanI	GGYRCC	2 cut(s)	1246, 1395
Bbv12I	GWGCWC	1 cut(s)	290
BbvI	GCAGC	3 cut(s)	295, 491, 969
BccI	CCATC	6 cut(s)	649, 919, 925, 1024, 1232, 1276
BceAI	ACGGC	2 cut(s)	59, 1492
BcgI	CGANNNNNNTGC	2 cut(s)	468, 502
BciT130I	CCWGG	4 cut(s)	282, 373, 401, 500
BclI	TGATCA	3 cut(s)	66, 696, 832
BcnI	CCSGG	3 cut(s)	141, 1339, 1406
BcoDI	GTCTC	2 cut(s)	1343, 1494
BfaI	CTAG	1 cut(s)	47
BfmI	CTRYAG	1 cut(s)	294
BglI	GCCNNNNNGGC	1 cut(s)	1343
BisI	GCNGC	5 cut(s)	149, 309, 480, 983, 1363
BlsI	GCNGC	5 cut(s)	150, 310, 481, 984, 1364
BmcAI	AGTACT	1 cut(s)	51
Bme1390I	CCNGG	7 cut(s)	141, 282, 373, 401, 500, 1339, 1406
Bme18I	GGWCC	3 cut(s)	278, 323, 704
BmeRI	GACNNNNNGTC	1 cut(s)	652
BmgT120I	GGNCC	4 cut(s)	278, 323, 704, 1084
BmiI	GGNNCC	6 cut(s)	324, 325, 349, 521, 1248, 1397
BmrFI	CCNGG	7 cut(s)	141, 282, 373, 401, 500, 1339, 1406
BmrI	ACTGGG	2 cut(s)	119, 329
BmsI	GCATC	1 cut(s)	251
BmuI	ACTGGG	2 cut(s)	119, 329
BpmI	CTGGAG	1 cut(s)	422
Bpu14I	TTCGAA	1 cut(s)	337
BpuMI	CCSGG	3 cut(s)	141, 1339, 1406
BsaBI	GATNNNNATC	1 cut(s)	634
BsaHI	GRCGYC	1 cut(s)	1137
BsaJI	CCNNGG	5 cut(s)	281, 444, 977, 1304, 1474
BsaXI	ACNNNNNCTCC	4 cut(s)	353, 383, 916, 946
Bsc4I	CCNNNNNNNGG	4 cut(s)	197, 198, 1133, 1405
Bse118I	RCCGGY	3 cut(s)	151, 173, 1249
Bse1I	ACTGG	4 cut(s)	114, 221, 324, 410
Bse3DI	GCAATG	2 cut(s)	349, 835
Bse8I	GATNNNNATC	1 cut(s)	634
BseBI	CCWGG	4 cut(s)	282, 373, 401, 500
BseDI	CCNNGG	5 cut(s)	281, 444, 977, 1304, 1474
BseGI	GGATG	4 cut(s)	635, 729, 1078, 1243
BseJI	GATNNNNATC	1 cut(s)	634
BseLI	CCNNNNNNNGG	4 cut(s)	197, 198, 1133, 1405
BseMI	GCAATG	2 cut(s)	349, 835
BseNI	ACTGG	4 cut(s)	114, 221, 324, 410
BseRI	GAGGAG	1 cut(s)	640
BseSI	GKGCMC	1 cut(s)	1398
BseXI	GCAGC	3 cut(s)	295, 491, 969
BseYI	CCCAGC	1 cut(s)	585
BsgI	GTGCAG	3 cut(s)	119, 1018, 1150
Bsh1285I	CGRYCG	2 cut(s)	469, 610
BshFI	GGCC	6 cut(s)	206, 573, 1085, 1337, 1365, 1479
BshNI	GGYRCC	2 cut(s)	1246, 1395
BsiEI	CGRYCG	2 cut(s)	469, 610
BsiHKAI	GWGCWC	1 cut(s)	290
BsiSI	CCGG	6 cut(s)	141, 152, 174, 1250, 1338, 1406
BsiWI	CGTACG	1 cut(s)	1353
BslFI	GGGAC	3 cut(s)	336, 566, 1388
BslI	CCNNNNNNNGG	4 cut(s)	197, 198, 1133, 1405
BsmAI	GTCTC	2 cut(s)	1343, 1494
BsmBI	CGTCTC	2 cut(s)	1343, 1494
BsmFI	GGGAC	3 cut(s)	336, 566, 1388
BsmI	GAATGC	2 cut(s)	244, 694
BsnI	GGCC	6 cut(s)	206, 573, 1085, 1337, 1365, 1479
Bsp119I	TTCGAA	1 cut(s)	337
Bsp1286I	GDGCHC	2 cut(s)	290, 1398
Bsp143I	GATC	8 cut(s)	66, 448, 466, 629, 696, 832, 907, 1027
Bsp19I	CCATGG	1 cut(s)	1304
BspACI	CCGC	6 cut(s)	149, 503, 624, 1227, 1346, 1362
BspANI	GGCC	6 cut(s)	206, 573, 1085, 1337, 1365, 1479
BspHI	TCATGA	2 cut(s)	678, 960
BspLI	GGNNCC	6 cut(s)	324, 325, 349, 521, 1248, 1397
BspPI	GGATC	3 cut(s)	456, 902, 1022
BspT104I	TTCGAA	1 cut(s)	337
BspT107I	GGYRCC	2 cut(s)	1246, 1395
BsrDI	GCAATG	2 cut(s)	349, 835
BsrFI	RCCGGY	3 cut(s)	151, 173, 1249
BsrI	ACTGG	4 cut(s)	114, 221, 324, 410
BssAI	RCCGGY	3 cut(s)	151, 173, 1249
BssECI	CCNNGG	5 cut(s)	281, 444, 977, 1304, 1474
BssMI	GATC	8 cut(s)	66, 448, 466, 629, 696, 832, 907, 1027
BssNI	GRCGYC	1 cut(s)	1137
BssT1I	CCWWGG	2 cut(s)	444, 1304
Bst2UI	CCWGG	4 cut(s)	282, 373, 401, 500
Bst4CI	ACNGT	4 cut(s)	719, 881, 1171, 1381
Bst6I	CTCTTC	1 cut(s)	816
BstACI	GRCGYC	1 cut(s)	1137
BstBI	TTCGAA	1 cut(s)	337
BstC8I	GCNNGC	5 cut(s)	175, 571, 591, 1204, 1208
BstDSI	CCRYGG	2 cut(s)	1304, 1474
BstEII	GGTNACC	1 cut(s)	127
BstF5I	GGATG	4 cut(s)	635, 729, 1078, 1243
BstHHI	GCGC	2 cut(s)	313, 1391
BstKTI	GATC	8 cut(s)	69, 451, 469, 632, 699, 835, 910, 1030
BstMAI	GTCTC	2 cut(s)	1343, 1494
BstMBI	GATC	8 cut(s)	66, 448, 466, 629, 696, 832, 907, 1027
BstMCI	CGRYCG	2 cut(s)	469, 610
BstMWI	GCNNNNNNNGC	5 cut(s)	145, 305, 570, 1091, 1343
BstNI	CCWGG	4 cut(s)	282, 373, 401, 500
BstNSI	RCATGY	1 cut(s)	1094
BstPI	GGTNACC	1 cut(s)	127
BstSCI	CCNGG	7 cut(s)	139, 280, 371, 399, 498, 1337, 1404
BstSFI	CTRYAG	1 cut(s)	294
BstSLI	GKGCMC	1 cut(s)	1398
BstV1I	GCAGC	3 cut(s)	295, 491, 969
BstX2I	RGATCY	1 cut(s)	448
BstYI	RGATCY	1 cut(s)	448
BsuRI	GGCC	6 cut(s)	206, 573, 1085, 1337, 1365, 1479
BtgI	CCRYGG	2 cut(s)	1304, 1474
BtsCI	GGATG	4 cut(s)	635, 729, 1078, 1243
BtsI	GCAGTG	1 cut(s)	1138
BtsIMutI	CAGTG	4 cut(s)	724, 903, 1138, 1167
Cac8I	GCNNGC	5 cut(s)	175, 571, 591, 1204, 1208
CaiI	CAGNNNCTG	1 cut(s)	482
CciI	TCATGA	2 cut(s)	678, 960
CfoI	GCGC	2 cut(s)	313, 1391
Cfr10I	RCCGGY	3 cut(s)	151, 173, 1249
Cfr13I	GGNCC	4 cut(s)	278, 323, 704, 1084
CseI	GACGC	1 cut(s)	1126
Csp6I	GTAC	6 cut(s)	50, 456, 800, 1172, 1267, 1354
CviAII	CATG	9 cut(s)	83, 679, 738, 746, 961, 995, 1091, 1305, 1314
CviQI	GTAC	6 cut(s)	50, 456, 800, 1172, 1267, 1354
DpnI	GATC	8 cut(s)	68, 450, 468, 631, 698, 834, 909, 1029
DpnII	GATC	8 cut(s)	66, 448, 466, 629, 696, 832, 907, 1027
DrdI	GACNNNNNNGTC	1 cut(s)	779
DriI	GACNNNNNGTC	1 cut(s)	652
DseDI	GACNNNNNNGTC	1 cut(s)	779
EaeI	YGGCCR	2 cut(s)	571, 1335
Eam1104I	CTCTTC	1 cut(s)	816
Eam1105I	GACNNNNNGTC	1 cut(s)	652
EarI	CTCTTC	1 cut(s)	816
EciI	GGCGGA	2 cut(s)	518, 1361
Eco130I	CCWWGG	2 cut(s)	444, 1304
Eco147I	AGGCCT	1 cut(s)	206
Eco47I	GGWCC	3 cut(s)	278, 323, 704
Eco91I	GGTNACC	1 cut(s)	127
EcoO109I	RGGNCCY	3 cut(s)	278, 323, 1084
EcoO65I	GGTNACC	1 cut(s)	127
EcoRII	CCWGG	4 cut(s)	280, 371, 399, 498
EcoT14I	CCWWGG	2 cut(s)	444, 1304
EcoT22I	ATGCAT	1 cut(s)	244
ErhI	CCWWGG	2 cut(s)	444, 1304
Esp3I	CGTCTC	2 cut(s)	1343, 1494
FaeI	CATG	9 cut(s)	86, 682, 741, 749, 964, 998, 1094, 1308, 1317
FaqI	GGGAC	3 cut(s)	336, 566, 1388
FatI	CATG	9 cut(s)	82, 678, 737, 745, 960, 994, 1090, 1304, 1313
FbaI	TGATCA	3 cut(s)	66, 696, 832
Fnu4HI	GCNGC	5 cut(s)	149, 309, 480, 983, 1363
FokI	GGATG	4 cut(s)	622, 736, 1085, 1250
Fsp4HI	GCNGC	5 cut(s)	149, 309, 480, 983, 1363
FspBI	CTAG	1 cut(s)	47
FspI	TGCGCA	1 cut(s)	312
GlaI	GCGC	2 cut(s)	312, 1390
GluI	GCNGC	5 cut(s)	149, 309, 480, 983, 1363
GsaI	CCCAGC	1 cut(s)	589
GsuI	CTGGAG	1 cut(s)	422
HaeIII	GGCC	6 cut(s)	206, 573, 1085, 1337, 1365, 1479
HapII	CCGG	6 cut(s)	141, 152, 174, 1250, 1338, 1406
HgaI	GACGC	1 cut(s)	1126
HhaI	GCGC	2 cut(s)	313, 1391
Hin1I	GRCGYC	1 cut(s)	1137
Hin1II	CATG	9 cut(s)	86, 682, 741, 749, 964, 998, 1094, 1308, 1317
Hin6I	GCGC	2 cut(s)	311, 1389
HinP1I	GCGC	2 cut(s)	311, 1389
HinfI	GANTC	6 cut(s)	404, 471, 485, 647, 742, 1157
HpaII	CCGG	6 cut(s)	141, 152, 174, 1250, 1338, 1406
HphI	GGTGA	5 cut(s)	121, 131, 399, 1241, 1393
Hpy166II	GTNNAC	1 cut(s)	802
Hpy188I	TCNGA	5 cut(s)	185, 646, 760, 1080, 1188
Hpy188III	TCNNGA	5 cut(s)	246, 679, 784, 961, 1195
Hpy8I	GTNNAC	1 cut(s)	802
Hpy99I	CGWCG	1 cut(s)	1381
HpyAV	CCTTC	7 cut(s)	110, 196, 538, 856, 1075, 1147, 1494
HpyCH4III	ACNGT	4 cut(s)	719, 881, 1171, 1381
HpyCH4IV	ACGT	3 cut(s)	180, 579, 1352
HpyCH4V	TGCA	8 cut(s)	100, 242, 828, 890, 974, 1035, 1131, 1415
HpyF10VI	GCNNNNNNNGC	5 cut(s)	145, 305, 570, 1091, 1343
HpySE526I	ACGT	3 cut(s)	180, 579, 1352
Hsp92I	GRCGYC	1 cut(s)	1137
Hsp92II	CATG	9 cut(s)	86, 682, 741, 749, 964, 998, 1094, 1308, 1317
HspAI	GCGC	2 cut(s)	311, 1389
KflI	GGGWCCC	1 cut(s)	323
KroI	GCCGGC	1 cut(s)	173
KroNI	GCCGGC	1 cut(s)	175
Ksp22I	TGATCA	3 cut(s)	66, 696, 832
Kzo9I	GATC	8 cut(s)	66, 448, 466, 629, 696, 832, 907, 1027
LmnI	GCTCC	1 cut(s)	305
Lsp1109I	GCAGC	3 cut(s)	295, 491, 969
LweI	GCATC	1 cut(s)	251
MaeI	CTAG	1 cut(s)	47
MaeII	ACGT	3 cut(s)	180, 579, 1352
MaeIII	GTNAC	6 cut(s)	119, 127, 405, 1229, 1252, 1425
MalI	GATC	8 cut(s)	68, 450, 468, 631, 698, 834, 909, 1029
MboI	GATC	8 cut(s)	66, 448, 466, 629, 696, 832, 907, 1027
MboII	GAAGA	3 cut(s)	98, 833, 1291
MflI	RGATCY	1 cut(s)	448
MhlI	GDGCHC	2 cut(s)	290, 1398
MlsI	TGGCCA	1 cut(s)	573
MluCI	AATT	8 cut(s)	16, 30, 89, 193, 354, 775, 856, 955
MluNI	TGGCCA	1 cut(s)	573
MlyI	GAGTC	4 cut(s)	413, 641, 751, 1151
MmeI	TCCRAC	2 cut(s)	208, 394
Mox20I	TGGCCA	1 cut(s)	573
Mph1103I	ATGCAT	1 cut(s)	244
MroNI	GCCGGC	1 cut(s)	173
MscI	TGGCCA	1 cut(s)	573
MseI	TTAA	6 cut(s)	9, 270, 360, 513, 951, 1289
MslI	CAYNNNNRTG	2 cut(s)	32, 1095
Msp20I	TGGCCA	1 cut(s)	573
MspA1I	CMGCKG	1 cut(s)	482
MspI	CCGG	6 cut(s)	141, 152, 174, 1250, 1338, 1406
MspR9I	CCNGG	7 cut(s)	141, 282, 373, 401, 500, 1339, 1406
Mva1269I	GAATGC	2 cut(s)	244, 694
MvaI	CCWGG	4 cut(s)	282, 373, 401, 500
MwoI	GCNNNNNNNGC	5 cut(s)	145, 305, 570, 1091, 1343
NaeI	GCCGGC	1 cut(s)	175
NciI	CCSGG	3 cut(s)	141, 1339, 1406
NcoI	CCATGG	1 cut(s)	1304
NdeII	GATC	8 cut(s)	66, 448, 466, 629, 696, 832, 907, 1027
NgoMIV	GCCGGC	1 cut(s)	173
NlaIII	CATG	9 cut(s)	86, 682, 741, 749, 964, 998, 1094, 1308, 1317
NlaIV	GGNNCC	6 cut(s)	324, 325, 349, 521, 1248, 1397
NmeAIII	GCCGAG	1 cut(s)	958
NmuCI	GTSAC	5 cut(s)	119, 127, 405, 1229, 1425
NsbI	TGCGCA	1 cut(s)	312
NsiI	ATGCAT	1 cut(s)	244
NspI	RCATGY	1 cut(s)	1094
NspV	TTCGAA	1 cut(s)	337
PagI	TCATGA	2 cut(s)	678, 960
PceI	AGGCCT	1 cut(s)	206
PcsI	WCGNNNNNNNCGW	1 cut(s)	177
PctI	GAATGC	2 cut(s)	244, 694
PdiI	GCCGGC	1 cut(s)	175
PfeI	GAWTC	2 cut(s)	471, 485
Pfl23II	CGTACG	1 cut(s)	1353
PflFI	GACNNNGTC	1 cut(s)	125
PfoI	TCCNGGA	2 cut(s)	371, 399
PkrI	GCNGC	5 cut(s)	150, 310, 481, 984, 1364
Ple19I	CGATCG	1 cut(s)	469
PleI	GAGTC	4 cut(s)	412, 641, 750, 1151
PpsI	GAGTC	4 cut(s)	412, 641, 750, 1151
PpuMI	RGGWCCY	2 cut(s)	278, 323
PshBI	ATTAAT	1 cut(s)	951
Psp5II	RGGWCCY	2 cut(s)	278, 323
Psp6I	CCWGG	4 cut(s)	280, 371, 399, 498
PspEI	GGTNACC	1 cut(s)	127
PspFI	CCCAGC	1 cut(s)	585
PspGI	CCWGG	4 cut(s)	280, 371, 399, 498
PspLI	CGTACG	1 cut(s)	1353
PspN4I	GGNNCC	6 cut(s)	324, 325, 349, 521, 1248, 1397
PspPI	GGNCC	4 cut(s)	278, 323, 704, 1084
PspPPI	RGGWCCY	2 cut(s)	278, 323
PstNI	CAGNNNCTG	1 cut(s)	482
PsuI	RGATCY	1 cut(s)	448
PsyI	GACNNNGTC	1 cut(s)	125
PvuI	CGATCG	1 cut(s)	469
PvuII	CAGCTG	1 cut(s)	482
RsaI	GTAC	6 cut(s)	51, 457, 801, 1173, 1268, 1355
RsaNI	GTAC	6 cut(s)	50, 456, 800, 1172, 1267, 1354
RseI	CAYNNNNRTG	2 cut(s)	32, 1095
SaqAI	TTAA	6 cut(s)	9, 270, 360, 513, 951, 1289
SatI	GCNGC	5 cut(s)	149, 309, 480, 983, 1363
Sau3AI	GATC	8 cut(s)	66, 448, 466, 629, 696, 832, 907, 1027
Sau96I	GGNCC	4 cut(s)	278, 323, 704, 1084
ScaI	AGTACT	1 cut(s)	51
SchI	GAGTC	4 cut(s)	413, 641, 751, 1151
ScrFI	CCNGG	7 cut(s)	141, 282, 373, 401, 500, 1339, 1406
SduI	GDGCHC	2 cut(s)	290, 1398
SfaNI	GCATC	1 cut(s)	251
SfcI	CTRYAG	1 cut(s)	294
SfuI	TTCGAA	1 cut(s)	337
SgrAI	CRCCGGYG	1 cut(s)	151
SinI	GGWCC	3 cut(s)	278, 323, 704
SmiMI	CAYNNNNRTG	2 cut(s)	32, 1095
Sse9I	AATT	8 cut(s)	16, 30, 89, 193, 354, 775, 856, 955
SseBI	AGGCCT	1 cut(s)	206
SsiI	CCGC	6 cut(s)	149, 503, 624, 1227, 1346, 1362
SspI	AATATT	1 cut(s)	1058
SspMI	CTAG	1 cut(s)	47
StuI	AGGCCT	1 cut(s)	206
StyD4I	CCNGG	7 cut(s)	139, 280, 371, 399, 498, 1337, 1404
StyI	CCWWGG	2 cut(s)	444, 1304
TaaI	ACNGT	4 cut(s)	719, 881, 1171, 1381
TaiI	ACGT	3 cut(s)	183, 582, 1355
TaqI	TCGA	6 cut(s)	247, 337, 469, 619, 841, 870
TaqII	GACCGA	2 cut(s)	624, 721
TasI	AATT	8 cut(s)	16, 30, 89, 193, 354, 775, 856, 955
TatI	WGTACW	2 cut(s)	49, 1171
TauI	GCSGC	2 cut(s)	151, 1365
TfiI	GAWTC	2 cut(s)	471, 485
Tru1I	TTAA	6 cut(s)	9, 270, 360, 513, 951, 1289
Tru9I	TTAA	6 cut(s)	9, 270, 360, 513, 951, 1289
TscAI	CASTG	4 cut(s)	724, 910, 1138, 1174
TseFI	GTSAC	5 cut(s)	119, 127, 405, 1229, 1425
TseI	GCWGC	3 cut(s)	308, 479, 982
Tsp45I	GTSAC	5 cut(s)	119, 127, 405, 1229, 1425
TspDTI	ATGAA	5 cut(s)	99, 649, 667, 949, 977
TspGWI	ACGGA	1 cut(s)	1250
TspRI	CASTG	4 cut(s)	724, 910, 1138, 1174
Tth111I	GACNNNGTC	1 cut(s)	125
VpaK11BI	GGWCC	3 cut(s)	278, 323, 704
VspI	ATTAAT	1 cut(s)	951
XapI	RAATTY	1 cut(s)	89
XceI	RCATGY	1 cut(s)	1094
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1311
XspI	CTAG	1 cut(s)	47
ZrmI	AGTACT	1 cut(s)	51
Zsp2I	ATGCAT	1 cut(s)	244

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.