Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0020147

Physical Location & Seq

Genomic Coordinates Reverse (-)

273991 .. 280268

Gene Structure 6278 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1004500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1503 bp

ATGGCCCTTAATCCTAATTTGTTGAACATGGCTATGATATGGTGTCTAGTACTACTTTTTCCTGTGACCAACGCCGTGAAACATGAAGAAGTAGAAACGCAGGGACAACTGGGAGAAGGTGACAGGGTCACCAACTTGCCCGGACAGCCGCCGGTGTCATTCCCGCACTACGCCGGCTACGTTCAACTTCCCAAATCCGGCAAGGCCTTGTTCTACTGGTTTTTCCAAGCTCAAGAGAATGCATCTCGGAAACCCCTTGTTCTTTGGCTTAATGGAGGACCTGGGTGCTCATCCGTAGCTTATGGAGCTGCACAAGAACTTGGCCCCTTTCTTGTTCGAAGCAATGGAACCCTAATTCTTAACAATTACTCCTGGAACAAAGTTGCCAATGTGCTGTTCCTGGAGTCACCTGCTGGAGTAGGGTTTTCCTACACAAACAACTCCAAGGATCTGCGTACGCTGGGCGATCGAATCACAGCTGCTGATTCGCATGCTTTCCTGGTGGAATGGTTTAAGAAGTTCCCAACCTTAAAATCCCATGATTTCTACATTGCTGGGGAGAGCTATGCTGGCCATTACGTCCCCCAGCTTGCCAACCTCATATACGACCGAAACAAAGGAGCCACCAGATCATCATCCCCATCAGAGTATTCCTCCTCTTTCATAAATCTGAAAGGTTTCATGATTGGGAATGCTGTGATCAATGAACCGACGGACAACAGTGGGATATTTGATTACGCATGGAGCCATGCCATAATCTCGGACACGCTCCACAACAATTTAGTAAATAAATGTGATGTCATACATGATGACAACCAAACAGAAGAGTGCAATGAACACATTCGAGCCTTCTTACAAGATTATTCTCAAATCGACATCTACGGCATTTATGCACCCATCTGCCTCACTGATCCATCTCCATCTACTACTGCTTCTCCCAAACCAACAAGGATTAACAATTTCATGAAAAATGTTGCACCTCGTCTGCTCAATCAACATGAGCTTTGGCAGAGGCTACCATCAGGGTATGATCCCTGCACAGAGGATTATGTTGAGCAATATTTCAACCGAGAGGATGTTCAGAGGGCCCTGCATGCCAATGTTACTAAACTTTCCTATCCTTACTCCCCATGCAGCGGCATCATAAAGGAATGGAAGGACTCGCCTGATACCGTACTGCCAATCATTCAGAAGCTCTTGAAAGCTGGCTTGCGTATTTGGATCTATAGTGGTGACACGGATGGGAGGGTGCCGGTAACATCAACGAGGTACAGCATAAAGAAGATGGGTTTAAGAGTAATTCAAGGATGGAAGGCATGGTTTGATAAGGGTCAAGTGGCCGGGTGGGCGGAGACGTACGAGGGCGGGCTCACGTTTGCGACGGTGAGGGGCGCAGGGCACCAAGTCCCGGTGTTTGCACCTCGGCAGTCTCTTTCTCTGTTTACCCATTTCCTCCATCCCAATCCCAACACCACCCACGGCCTCCCTTCTTCTCGTTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004180 GO:0004185 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0009056 GO:0009057 GO:0009987 GO:0016787 GO:0017171 GO:0019538 GO:0030163 GO:0043170 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0070008 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_S10

Protein Analysis

501

Amino Acids

55.96

Weight (kDa)

6.94

Isoelectric Point (pI)

43.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S10	PF00450	47 - 486	2.9e-139	Serine carboxypeptidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0020147)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08260
malus_domestica	MD13G1004500.v1.1 MD16G1003100.v1.1
prunus_persica	Prupe.1G348100_v2.0.a1
pyrus_communis	pycom13g00390 pycom16g00300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	418
Acc36I	ACCTGC	1 cut(s)	418
AccB1I	GGYRCC	2 cut(s)	1249, 1398
AciI	CCGC	5 cut(s)	149, 164, 1137, 1349, 1365
AclWI	GGATC	4 cut(s)	456, 905, 1025, 1229
AcoI	YGGCCR	2 cut(s)	571, 1338
AfaI	GTAC	5 cut(s)	51, 457, 1176, 1271, 1358
AfiI	CCNNNNNNNGG	3 cut(s)	197, 1136, 1408
AgsI	TTSAA	5 cut(s)	25, 185, 1066, 1201, 1304
AjnI	CCWGG	4 cut(s)	280, 371, 399, 498
AjuI	GAANNNNNNNTTGG	2 cut(s)	380, 412
AluBI	AGCT	9 cut(s)	230, 299, 308, 479, 564, 589, 1003, 1195, 1205
AluI	AGCT	9 cut(s)	230, 299, 308, 479, 564, 589, 1003, 1195, 1205
Alw21I	GWGCWC	1 cut(s)	290
Alw26I	GTCTC	2 cut(s)	1346, 1434
AlwI	GGATC	4 cut(s)	456, 905, 1025, 1229
AlwNI	CAGNNNCTG	1 cut(s)	482
AoxI	GGCC	7 cut(s)	3, 204, 322, 571, 1086, 1338, 1480
ApaI	GGGCCC	1 cut(s)	1090
ApeKI	GCWGC	3 cut(s)	308, 479, 1134
Asp700I	GAANNNNTTC	2 cut(s)	677, 840
AspLEI	GCGC	1 cut(s)	1394
AspS9I	GGNCC	5 cut(s)	4, 278, 323, 1086, 1087
AsuC2I	CCSGG	3 cut(s)	141, 1342, 1409
AsuHPI	GGTGA	5 cut(s)	121, 131, 399, 1244, 1396
AsuII	TTCGAA	1 cut(s)	337
AvaII	GGWCC	1 cut(s)	278
BaeGI	GKGCMC	2 cut(s)	1090, 1401
BalI	TGGCCA	1 cut(s)	573
BanI	GGYRCC	2 cut(s)	1249, 1398
BanII	GRGCYC	2 cut(s)	1090, 1371
Bbv12I	GWGCWC	1 cut(s)	290
BbvI	GCAGC	3 cut(s)	295, 466, 1146
BccI	CCATC	9 cut(s)	649, 905, 922, 928, 1027, 1235, 1279, 1302, 1464
BceAI	ACGGC	3 cut(s)	59, 898, 1495
BciT130I	CCWGG	4 cut(s)	282, 373, 401, 500
BclI	TGATCA	1 cut(s)	699
BcnI	CCSGG	3 cut(s)	141, 1342, 1409
BcoDI	GTCTC	2 cut(s)	1346, 1434
BfaI	CTAG	1 cut(s)	47
BfmI	CTRYAG	1 cut(s)	1225
BfuAI	ACCTGC	1 cut(s)	418
BglI	GCCNNNNNGGC	1 cut(s)	1346
BisI	GCNGC	5 cut(s)	149, 309, 480, 1135, 1138
BlsI	GCNGC	5 cut(s)	150, 310, 481, 1136, 1139
BmcAI	AGTACT	1 cut(s)	51
Bme1390I	CCNGG	7 cut(s)	141, 282, 373, 401, 500, 1342, 1409
Bme18I	GGWCC	1 cut(s)	278
BmgT120I	GGNCC	5 cut(s)	4, 278, 323, 1086, 1087
BmiI	GGNNCC	7 cut(s)	325, 349, 622, 746, 1088, 1251, 1400
BmrFI	CCNGG	7 cut(s)	141, 282, 373, 401, 500, 1342, 1409
BmrI	ACTGGG	1 cut(s)	119
BmsI	GCATC	2 cut(s)	251, 1149
BmuI	ACTGGG	1 cut(s)	119
BplI	GAGNNNNNCTC	2 cut(s)	638, 670
BpmI	CTGGAG	2 cut(s)	422, 435
Bpu14I	TTCGAA	1 cut(s)	337
BpuEI	CTTGAG	1 cut(s)	216
BpuMI	CCSGG	3 cut(s)	141, 1342, 1409
BsaBI	GATNNNNATC	1 cut(s)	634
BsaJI	CCNNGG	4 cut(s)	281, 444, 1421, 1477
BsaXI	ACNNNNNCTCC	4 cut(s)	353, 383, 919, 949
Bsc4I	CCNNNNNNNGG	3 cut(s)	197, 1136, 1408
Bse118I	RCCGGY	3 cut(s)	151, 173, 1252
Bse1I	ACTGG	2 cut(s)	114, 221
Bse3DI	GCAATG	3 cut(s)	349, 549, 838
Bse8I	GATNNNNATC	1 cut(s)	634
BseBI	CCWGG	4 cut(s)	282, 373, 401, 500
BseDI	CCNNGG	4 cut(s)	281, 444, 1421, 1477
BseGI	GGATG	6 cut(s)	290, 635, 1081, 1246, 1313, 1456
BseJI	GATNNNNATC	1 cut(s)	634
BseLI	CCNNNNNNNGG	3 cut(s)	197, 1136, 1408
BseMI	GCAATG	3 cut(s)	349, 549, 838
BseNI	ACTGG	2 cut(s)	114, 221
BseRI	GAGGAG	1 cut(s)	646
BseSI	GKGCMC	2 cut(s)	1090, 1401
BseXI	GCAGC	3 cut(s)	295, 466, 1146
BseYI	CCCAGC	3 cut(s)	460, 554, 585
BsgI	GTGCAG	2 cut(s)	294, 1021
Bsh1285I	CGRYCG	2 cut(s)	469, 610
BshFI	GGCC	7 cut(s)	5, 206, 324, 573, 1088, 1340, 1482
BshNI	GGYRCC	2 cut(s)	1249, 1398
BsiEI	CGRYCG	2 cut(s)	469, 610
BsiHKAI	GWGCWC	1 cut(s)	290
BsiSI	CCGG	7 cut(s)	141, 152, 174, 198, 1253, 1341, 1409
BsiWI	CGTACG	2 cut(s)	455, 1356
BslFI	GGGAC	3 cut(s)	117, 566, 1391
BslI	CCNNNNNNNGG	3 cut(s)	197, 1136, 1408
BsmAI	GTCTC	2 cut(s)	1346, 1434
BsmBI	CGTCTC	1 cut(s)	1346
BsmFI	GGGAC	3 cut(s)	117, 566, 1391
BsmI	GAATGC	2 cut(s)	244, 697
BsnI	GGCC	7 cut(s)	5, 206, 324, 573, 1088, 1340, 1482
Bsp119I	TTCGAA	1 cut(s)	337
Bsp120I	GGGCCC	1 cut(s)	1086
Bsp1286I	GDGCHC	4 cut(s)	290, 1090, 1371, 1401
Bsp143I	GATC	7 cut(s)	448, 466, 629, 699, 910, 1030, 1221
BspACI	CCGC	5 cut(s)	149, 164, 1137, 1349, 1365
BspANI	GGCC	7 cut(s)	5, 206, 324, 573, 1088, 1340, 1482
BspHI	TCATGA	2 cut(s)	681, 963
BspLI	GGNNCC	7 cut(s)	325, 349, 622, 746, 1088, 1251, 1400
BspMI	ACCTGC	1 cut(s)	418
BspPI	GGATC	4 cut(s)	456, 905, 1025, 1229
BspT104I	TTCGAA	1 cut(s)	337
BspT107I	GGYRCC	2 cut(s)	1249, 1398
BsrDI	GCAATG	3 cut(s)	349, 549, 838
BsrFI	RCCGGY	3 cut(s)	151, 173, 1252
BsrI	ACTGG	2 cut(s)	114, 221
BssAI	RCCGGY	3 cut(s)	151, 173, 1252
BssECI	CCNNGG	4 cut(s)	281, 444, 1421, 1477
BssMI	GATC	7 cut(s)	448, 466, 629, 699, 910, 1030, 1221
BssT1I	CCWWGG	1 cut(s)	444
Bst2UI	CCWGG	4 cut(s)	282, 373, 401, 500
Bst4CI	ACNGT	3 cut(s)	722, 1174, 1384
Bst6I	CTCTTC	1 cut(s)	819
BstBI	TTCGAA	1 cut(s)	337
BstC8I	GCNNGC	8 cut(s)	175, 492, 571, 591, 1095, 1207, 1211, 1367
BstDSI	CCRYGG	1 cut(s)	1477
BstEII	GGTNACC	1 cut(s)	127
BstF5I	GGATG	6 cut(s)	290, 635, 1081, 1246, 1313, 1456
BstHHI	GCGC	1 cut(s)	1394
BstKTI	GATC	7 cut(s)	451, 469, 632, 702, 913, 1033, 1224
BstMAI	GTCTC	2 cut(s)	1346, 1434
BstMBI	GATC	7 cut(s)	448, 466, 629, 699, 910, 1030, 1221
BstMCI	CGRYCG	2 cut(s)	469, 610
BstMWI	GCNNNNNNNGC	5 cut(s)	145, 305, 570, 1094, 1346
BstNI	CCWGG	4 cut(s)	282, 373, 401, 500
BstNSI	RCATGY	2 cut(s)	494, 1097
BstPI	GGTNACC	1 cut(s)	127
BstSCI	CCNGG	7 cut(s)	139, 280, 371, 399, 498, 1340, 1407
BstSFI	CTRYAG	1 cut(s)	1225
BstSLI	GKGCMC	2 cut(s)	1090, 1401
BstV1I	GCAGC	3 cut(s)	295, 466, 1146
BstX2I	RGATCY	2 cut(s)	448, 1221
BstYI	RGATCY	2 cut(s)	448, 1221
BsuRI	GGCC	7 cut(s)	5, 206, 324, 573, 1088, 1340, 1482
BtgI	CCRYGG	1 cut(s)	1477
BtsCI	GGATG	6 cut(s)	290, 635, 1081, 1246, 1313, 1456
BtsIMutI	CAGTG	2 cut(s)	727, 906
BveI	ACCTGC	1 cut(s)	418
Cac8I	GCNNGC	8 cut(s)	175, 492, 571, 591, 1095, 1207, 1211, 1367
CaiI	CAGNNNCTG	1 cut(s)	482
CciI	TCATGA	2 cut(s)	681, 963
CfoI	GCGC	1 cut(s)	1394
Cfr10I	RCCGGY	3 cut(s)	151, 173, 1252
Cfr13I	GGNCC	5 cut(s)	4, 278, 323, 1086, 1087
Csp6I	GTAC	5 cut(s)	50, 456, 1175, 1270, 1357
CviQI	GTAC	5 cut(s)	50, 456, 1175, 1270, 1357
DpnI	GATC	7 cut(s)	450, 468, 631, 701, 912, 1032, 1223
DpnII	GATC	7 cut(s)	448, 466, 629, 699, 910, 1030, 1221
EaeI	YGGCCR	2 cut(s)	571, 1338
Eam1104I	CTCTTC	1 cut(s)	819
EarI	CTCTTC	1 cut(s)	819
EciI	GGCGGA	1 cut(s)	1364
Eco130I	CCWWGG	1 cut(s)	444
Eco147I	AGGCCT	1 cut(s)	206
Eco24I	GRGCYC	2 cut(s)	1090, 1371
Eco47I	GGWCC	1 cut(s)	278
Eco91I	GGTNACC	1 cut(s)	127
EcoO109I	RGGNCCY	3 cut(s)	278, 1086, 1087
EcoO65I	GGTNACC	1 cut(s)	127
EcoRII	CCWGG	4 cut(s)	280, 371, 399, 498
EcoT14I	CCWWGG	1 cut(s)	444
EcoT22I	ATGCAT	1 cut(s)	244
EcoT38I	GRGCYC	2 cut(s)	1090, 1371
ErhI	CCWWGG	1 cut(s)	444
Esp3I	CGTCTC	1 cut(s)	1346
FaqI	GGGAC	3 cut(s)	117, 566, 1391
FauI	CCCGC	2 cut(s)	171, 1358
FbaI	TGATCA	1 cut(s)	699
Fnu4HI	GCNGC	5 cut(s)	149, 309, 480, 1135, 1138
FokI	GGATG	6 cut(s)	277, 622, 1088, 1253, 1320, 1443
FriOI	GRGCYC	2 cut(s)	1090, 1371
Fsp4HI	GCNGC	5 cut(s)	149, 309, 480, 1135, 1138
FspBI	CTAG	1 cut(s)	47
GlaI	GCGC	1 cut(s)	1393
GluI	GCNGC	5 cut(s)	149, 309, 480, 1135, 1138
GsaI	CCCAGC	3 cut(s)	464, 558, 589
GsuI	CTGGAG	2 cut(s)	422, 435
HaeIII	GGCC	7 cut(s)	5, 206, 324, 573, 1088, 1340, 1482
HapII	CCGG	7 cut(s)	141, 152, 174, 198, 1253, 1341, 1409
HhaI	GCGC	1 cut(s)	1394
Hin6I	GCGC	1 cut(s)	1392
HinP1I	GCGC	1 cut(s)	1392
HinfI	GANTC	4 cut(s)	404, 471, 485, 1160
HpaII	CCGG	7 cut(s)	141, 152, 174, 198, 1253, 1341, 1409
HphI	GGTGA	5 cut(s)	121, 131, 399, 1244, 1396
Hpy166II	GTNNAC	1 cut(s)	1443
Hpy188I	TCNGA	6 cut(s)	249, 646, 672, 763, 1083, 1191
Hpy188III	TCNNGA	4 cut(s)	233, 682, 964, 1198
Hpy8I	GTNNAC	1 cut(s)	1443
Hpy99I	CGWCG	2 cut(s)	715, 1384
HpyAV	CCTTC	5 cut(s)	110, 859, 1150, 1306, 1497
HpyCH4III	ACNGT	3 cut(s)	722, 1174, 1384
HpyCH4IV	ACGT	4 cut(s)	180, 579, 1355, 1373
HpyCH4V	TGCA	9 cut(s)	242, 311, 831, 893, 977, 1038, 1093, 1134, 1418
HpyF10VI	GCNNNNNNNGC	5 cut(s)	145, 305, 570, 1094, 1346
HpySE526I	ACGT	4 cut(s)	180, 579, 1355, 1373
HspAI	GCGC	1 cut(s)	1392
KroI	GCCGGC	1 cut(s)	173
KroNI	GCCGGC	1 cut(s)	175
Ksp22I	TGATCA	1 cut(s)	699
Kzo9I	GATC	7 cut(s)	448, 466, 629, 699, 910, 1030, 1221
LmnI	GCTCC	4 cut(s)	305, 620, 744, 774
Lsp1109I	GCAGC	3 cut(s)	295, 466, 1146
LweI	GCATC	2 cut(s)	251, 1149
MaeI	CTAG	1 cut(s)	47
MaeII	ACGT	4 cut(s)	180, 579, 1355, 1373
MaeIII	GTNAC	7 cut(s)	64, 119, 127, 405, 1102, 1232, 1255
MalI	GATC	7 cut(s)	450, 468, 631, 701, 912, 1032, 1223
MboI	GATC	7 cut(s)	448, 466, 629, 699, 910, 1030, 1221
MboII	GAAGA	4 cut(s)	98, 836, 1294, 1482
MflI	RGATCY	2 cut(s)	448, 1221
MhlI	GDGCHC	4 cut(s)	290, 1090, 1371, 1401
MlsI	TGGCCA	1 cut(s)	573
MluCI	AATT	6 cut(s)	16, 354, 364, 778, 958, 1299
MluNI	TGGCCA	1 cut(s)	573
MlyI	GAGTC	2 cut(s)	413, 1154
Mox20I	TGGCCA	1 cut(s)	573
Mph1103I	ATGCAT	1 cut(s)	244
MroNI	GCCGGC	1 cut(s)	173
MroXI	GAANNNNTTC	2 cut(s)	677, 840
MscI	TGGCCA	1 cut(s)	573
MseI	TTAA	7 cut(s)	9, 270, 360, 513, 530, 954, 1292
MslI	CAYNNNNRTG	2 cut(s)	32, 1098
Msp20I	TGGCCA	1 cut(s)	573
MspA1I	CMGCKG	2 cut(s)	479, 1137
MspI	CCGG	7 cut(s)	141, 152, 174, 198, 1253, 1341, 1409
MspR9I	CCNGG	7 cut(s)	141, 282, 373, 401, 500, 1342, 1409
Mva1269I	GAATGC	2 cut(s)	244, 697
MvaI	CCWGG	4 cut(s)	282, 373, 401, 500
MwoI	GCNNNNNNNGC	5 cut(s)	145, 305, 570, 1094, 1346
NaeI	GCCGGC	1 cut(s)	175
NciI	CCSGG	3 cut(s)	141, 1342, 1409
NdeII	GATC	7 cut(s)	448, 466, 629, 699, 910, 1030, 1221
NgoMIV	GCCGGC	1 cut(s)	173
NlaIV	GGNNCC	7 cut(s)	325, 349, 622, 746, 1088, 1251, 1400
NmeAIII	GCCGAG	1 cut(s)	1402
NmuCI	GTSAC	5 cut(s)	64, 119, 127, 405, 1232
NsiI	ATGCAT	1 cut(s)	244
NspI	RCATGY	2 cut(s)	494, 1097
NspV	TTCGAA	1 cut(s)	337
PaeI	GCATGC	2 cut(s)	494, 1097
PagI	TCATGA	2 cut(s)	681, 963
PaqCI	CACCTGC	1 cut(s)	418
PceI	AGGCCT	1 cut(s)	206
PcsI	WCGNNNNNNNCGW	1 cut(s)	177
PctI	GAATGC	2 cut(s)	244, 697
PdiI	GCCGGC	1 cut(s)	175
PdmI	GAANNNNTTC	2 cut(s)	677, 840
PfeI	GAWTC	2 cut(s)	471, 485
Pfl23II	CGTACG	2 cut(s)	455, 1356
PflFI	GACNNNGTC	1 cut(s)	125
PfoI	TCCNGGA	2 cut(s)	371, 399
PkrI	GCNGC	5 cut(s)	150, 310, 481, 1136, 1139
Ple19I	CGATCG	1 cut(s)	469
PleI	GAGTC	2 cut(s)	412, 1154
PpsI	GAGTC	2 cut(s)	412, 1154
PpuMI	RGGWCCY	1 cut(s)	278
Psp5II	RGGWCCY	1 cut(s)	278
Psp6I	CCWGG	4 cut(s)	280, 371, 399, 498
PspEI	GGTNACC	1 cut(s)	127
PspFI	CCCAGC	3 cut(s)	460, 554, 585
PspGI	CCWGG	4 cut(s)	280, 371, 399, 498
PspLI	CGTACG	2 cut(s)	455, 1356
PspN4I	GGNNCC	7 cut(s)	325, 349, 622, 746, 1088, 1251, 1400
PspOMI	GGGCCC	1 cut(s)	1086
PspPI	GGNCC	5 cut(s)	4, 278, 323, 1086, 1087
PspPPI	RGGWCCY	1 cut(s)	278
PstNI	CAGNNNCTG	1 cut(s)	482
PsuI	RGATCY	2 cut(s)	448, 1221
PsyI	GACNNNGTC	1 cut(s)	125
PvuI	CGATCG	1 cut(s)	469
PvuII	CAGCTG	1 cut(s)	479
RsaI	GTAC	5 cut(s)	51, 457, 1176, 1271, 1358
RsaNI	GTAC	5 cut(s)	50, 456, 1175, 1270, 1357
RseI	CAYNNNNRTG	2 cut(s)	32, 1098
SaqAI	TTAA	7 cut(s)	9, 270, 360, 513, 530, 954, 1292
SatI	GCNGC	5 cut(s)	149, 309, 480, 1135, 1138
Sau3AI	GATC	7 cut(s)	448, 466, 629, 699, 910, 1030, 1221
Sau96I	GGNCC	5 cut(s)	4, 278, 323, 1086, 1087
ScaI	AGTACT	1 cut(s)	51
SchI	GAGTC	2 cut(s)	413, 1154
ScrFI	CCNGG	7 cut(s)	141, 282, 373, 401, 500, 1342, 1409
SduI	GDGCHC	4 cut(s)	290, 1090, 1371, 1401
SfaNI	GCATC	2 cut(s)	251, 1149
SfcI	CTRYAG	1 cut(s)	1225
SfuI	TTCGAA	1 cut(s)	337
SgrAI	CRCCGGYG	1 cut(s)	151
SinI	GGWCC	1 cut(s)	278
SmiMI	CAYNNNNRTG	2 cut(s)	32, 1098
SmlI	CTYRAG	1 cut(s)	231
SmoI	CTYRAG	1 cut(s)	231
SphI	GCATGC	2 cut(s)	494, 1097
Sse9I	AATT	6 cut(s)	16, 354, 364, 778, 958, 1299
SseBI	AGGCCT	1 cut(s)	206
SsiI	CCGC	5 cut(s)	149, 164, 1137, 1349, 1365
SspI	AATATT	1 cut(s)	1061
SspMI	CTAG	1 cut(s)	47
StuI	AGGCCT	1 cut(s)	206
StyD4I	CCNGG	7 cut(s)	139, 280, 371, 399, 498, 1340, 1407
StyI	CCWWGG	1 cut(s)	444
TaaI	ACNGT	3 cut(s)	722, 1174, 1384
TaiI	ACGT	4 cut(s)	183, 582, 1358, 1376
TaqI	TCGA	4 cut(s)	337, 469, 844, 873
TaqII	GACCGA	1 cut(s)	624
TasI	AATT	6 cut(s)	16, 354, 364, 778, 958, 1299
TatI	WGTACW	1 cut(s)	49
TauI	GCSGC	2 cut(s)	151, 1140
TfiI	GAWTC	2 cut(s)	471, 485
Tru1I	TTAA	7 cut(s)	9, 270, 360, 513, 530, 954, 1292
Tru9I	TTAA	7 cut(s)	9, 270, 360, 513, 530, 954, 1292
TscAI	CASTG	2 cut(s)	727, 913
TseFI	GTSAC	5 cut(s)	64, 119, 127, 405, 1232
TseI	GCWGC	3 cut(s)	308, 479, 1134
Tsp45I	GTSAC	5 cut(s)	64, 119, 127, 405, 1232
TspDTI	ATGAA	7 cut(s)	99, 652, 670, 720, 849, 952, 980
TspGWI	ACGGA	3 cut(s)	283, 728, 1253
TspRI	CASTG	2 cut(s)	727, 913
Tth111I	GACNNNGTC	1 cut(s)	125
VpaK11BI	GGWCC	1 cut(s)	278
XceI	RCATGY	2 cut(s)	494, 1097
XmnI	GAANNNNTTC	2 cut(s)	677, 840
XspI	CTAG	1 cut(s)	47
ZrmI	AGTACT	1 cut(s)	51
Zsp2I	ATGCAT	1 cut(s)	244

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.